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高压氧对激素性股骨头坏死兔血清TXA2、PGI2及血液流变学的影响目的通过观察高压氧（HBO）治疗前后激素性股骨头坏死（SANFH）兔血清血栓素A2（TXA2）、前列腺素I2（PGI2）及血液流变学指标的变化，探讨HBO治疗SANFH的作用机制。
方法健康新西兰白兔正常对照组10只、模型组20只[非干预组（N-HPO）和HPO组各10只]，正常对照组和N-HPO组不予任何处理，高压氧组予以高压氧疗。
检测并比较各组血清血栓素B2（TXB2，TXA2的稳定代谢产物）、6-酮-前列腺素F1α（6-keto-PGF1α，PGI2的稳定代谢产物）含量及血液流变学的变化。
结果建模前模型组和对照组的血清TXB2、6-keto-PGF1α和血流变指标无明显差异（均P>0.05），建模成功后模型组的TXB2和血液流变学指标均显著高于对照组，而6-keto-PGF1α显著低于对照组，差异有统计学意义（均P＜0.01）；治疗前HPO组和N-HPO组的血清TXB2、6-keto-PGF1α和血液流变学指标无明显差异（均P>0.05），HPO治疗后2周、4周和6周模型组的TXB2和血液流变学指标均显著低于非HPO组，而6-keto-PGF1α显著高于非HPO组，差异有统计学意义（均P＜0.01）。
结论HBO 可纠正SANFH兔血中TXA2/PGI2失衡，降低血黏度，减轻股骨头损伤。
[Abstract] Objective To investigate the mechanism of action of hyperbaric oxygen（HBO）in treatment for SANFH in rabbit model with SANFH through assaying the serum concentration of thromboxane B2（TXA2）and prostaglandin I2（PGI2）and femoralhead. Methods Thirty-two New Zealand white rabbits were randomly divided into two groups according to table of random digit：normal control group（10 cases）and model group（22 cases）.The model group was subdivided into non-intervention group（10 cases）and HPO group（10 cases）after creating SANFH model through injection of endotoxin and methyl-prednisolone. Assayed and compared the difference of serum concentration of thromboxane B2（TXB2，stable metabolites of TXA2）and 6-keto prostaglandin F1α（6-keto-PGF1α，stable metabolites of PGI2）in three groups. Results There was no apparent difference of serum concentration of TXB2，6-keto-PGF1α and hemorheology index between control group and model group before creating model（all P>0.05）. The concentration of TXB2 and hemorheology index in model group was significantly higher than that in control group after creating model，whereas the concentration of 6-keto-PGF1α was significantly lower，there was significant difference between two groups（all P＜0.01）. There was no apparent difference of serum concentration of TXB2，6-keto-PGF1α and hemorheology index between non-HPO group and HPO group before treatment of HPO（all P>0.05）.The concentration of TXB2 and hemorheology index in HPO group was significantly lower than that in non-HPO group in second week，fouth week and sixth week after treatment of HPO，whereas the concentration of 6-keto-PGF1α was significantly higher，there was significant difference between two groups（all P＜0.01）. Results HPO can correct TXA2/PGI2 inballance in blood of rabbits with SANFH，lower blood viscosity and reduce the femoral head injury.[Keywords] Avascularnecrosis of the femoral head；Hormone；Hyperbaric oxygen；TXA2；PGI2；Hemorheology；Blood激素性股骨头坏死（steroid-induced avascular necrosis of the femoral head，SANFH）是临床骨科常见的难治疾病之一，其发病机制较为复杂[1]。
高压氧预防大鼠早期激素性股骨头坏死实验研究赵振广;潘树义;李航【摘要】Objective To investigate the effects of hyperbaric oxygenation ( HBO ) on preventing the steroid-induced avascular necrosis of the femoral head ( SANFH ) of mammals. Methods Distributing the 35 male Wistar rats into 3 groups: 5 rats for blank group, 15 rats for model group, and 15 rats for control group. The rats in the model group and the control group were injected the dexametha-sone sodium phosphate injection ( 10 mL/kg, 2 times/week ) in the abdominal cavity to built the model of SANFH. While establishing the rats model, the rats in the control group were given the HBO ( pressure:1. 8ATA, 1 time/day, 1 hour/time ) at the same time. And the blank group were injected normal saline. After 8 weeks, anteroposterior bilateral hip X-Ray examinations were performed in all of the rats. Then, executed them, took the femoral head out and the general observation and histological study were performed. Results There was no obvious abnormality in the results of the general observation and the X-Ray examinations. But there were visible differ-ences between model group and control group in the pathological sections. After stained with HE, the sections were observed under the light microscope: there were no focal osteonecrosis in the sections of the blank group and obvious focal osteonecrosis in the model group. And comparing with the model group, the osteocytes in the control group had a lighter damage. Conclusion HBO can prevent the damage of osteocytes during earlier period of SANFH.%目的：观察高压氧对动物激素性股骨头坏死的预防作用。
高氧液预处理对兔脊髓缺血-再灌注损伤的保护作用桑韩飞;徐礼鲜;张英民;王强;侯理朝;吴明春【期刊名称】《临床麻醉学杂志》【年(卷),期】2002(018)006【摘要】目的探讨高氧液预处理对兔脊髓缺血-再灌注损伤的作用.方法 20只成年雄性新西兰大白兔随机分成对照组(n＝10)及预处理组(n＝10).预处理组每天静脉给予10ml/kg高氧液,20分钟匀速泵完,连续5天;对照组用同样方法给予等容量生理盐水.最后一次预处理结束后24小时,夹闭腹主动脉肾下段20分钟,制作兔脊髓缺血模型;再灌注后4、8、12、24和48小时分别对动物神经功能评分;再灌注48小时后,处死动物取脊髓(L5～7),制作标本行组织病理学观察.结果预处理组神经功能评分在各时间点均明显高于对照组(P＜0.05);与对照组相比,预处理组脊髓前角正常神经细胞数明显增多(P＜0.05),而且神经功能评分与其对应脊髓前角正常神经细胞计数之间有显著相关性(r＝0.894,P＜0.01).结论高氧液预处理对兔脊髓缺血-再灌注损伤有显著的保护作用.【总页数】3页(P315-317)【作者】桑韩飞;徐礼鲜;张英民;王强;侯理朝;吴明春【作者单位】710032,西安市第四军医大学西京医院麻醉科;西安市第四军医大学口腔医学院麻醉科;710032,西安市第四军医大学西京医院麻醉科;710032,西安市第四军医大学西京医院麻醉科;710032,西安市第四军医大学西京医院麻醉科;710032,西安市第四军医大学西京医院麻醉科【正文语种】中文【中图分类】R65【相关文献】1.高氧液预处理对兔心肌缺血再灌注损伤的保护作用 [J], 景桂霞;温健;王伟2.高氧液对兔脊髓缺血再灌注损伤的保护作用 [J], 巩固;桑韩飞;张英民;陈敏;曾毅;熊利泽;劳宁3.高氧液预处理对兔脊髓缺血损伤的保护作用及机制 [J], 桑韩飞;张英民;徐礼鲜;程虹;巩固;梅其炳;熊利泽4.高氧液对兔脊髓缺血再灌注损伤的保护作用及对HSP90表达的影响 [J], 巩固;龚辉;张英民;陈敏;熊利泽;雷毅;郑恒兴;曾毅5.远程缺血预处理对兔脊髓缺血再灌注损伤的保护作用及其机制 [J], 余奇劲;田文华;杨云朝因版权原因，仅展示原文概要，查看原文内容请购买。
高压氧治疗在肌肉骨骼系统疾病康复中的促进作用高压氧治疗是一种通过让患者呼吸纯氧在高压力环境下进行的康复治疗方法。
它已被广泛应用于肌肉骨骼系统疾病的康复治疗中，并取得了显著的促进作用。
本文将探讨高压氧治疗的原理及其在肌肉骨骼系统疾病康复中的作用。
一、高压氧治疗的原理高压氧治疗即是在高压力环境下让患者呼吸纯氧。
在正常大气压下，氧的溶解度较低，进入组织和血液中的氧分子数量有限。
而在高压力环境下，氧的溶解度大大增加。
通过让患者在高压氧舱中呼吸纯氧，可以使血液中的氧含量迅速提高，进而促进组织的氧供应。
二、高压氧治疗在骨骼系统疾病康复中的作用1. 促进骨骼修复高压氧治疗在骨折康复中具有重要作用。
在高压氧环境下，血液中的氧浓度升高，有助于加快骨细胞的代谢和分裂。
同时，高压氧还能促进血管新生和增加毛细血管的通透性，这为骨骼修复提供了更好的条件。
研究表明，高压氧治疗能够明显加快骨折的愈合速度，并减轻疼痛感。
2. 缓解关节疾病症状关节疾病如骨关节炎常导致关节疼痛、肿胀和功能障碍。
高压氧治疗可以减轻关节炎患者的症状。
通过提高血液中的氧含量，高压氧能够缓解关节周围组织的炎症反应，并促进受损组织的修复。
研究表明，高压氧治疗可以显著减少关节炎患者的疼痛和肿胀，并提高关节的功能。
3. 促进软组织康复高压氧治疗在软组织损伤的康复中也具有积极的作用。
对于肌肉拉伤、韧带损伤等软组织损伤，高压氧可以加速组织的修复和重塑。
高压氧能够促进血液中氧供应的增加，从而改善组织的供血情况，加速受损组织的修复过程。
此外，高压氧还能抑制炎症反应，减轻肌肉和韧带的疼痛。
三、高压氧治疗的注意事项在使用高压氧治疗时，也需注意一些事项。
首先，高压氧治疗一般需要在专业的医疗机构进行，由专业医务人员负责操作。
其次，患者需要接受专业的评估，确定是否适合进行高压氧治疗。
对于一些存在心脏疾病、中耳炎等患者，高压氧治疗可能不适合。
此外，在高压氧治疗过程中，患者需要遵守医生的指导，并主动报告不适情况。
克骨汤对兔激素性股骨头坏死血管内皮生长因子表达影响的实验研究赵万军;杨武民;杨贺杰;夏志敏;周辉【摘要】目的观察克骨汤对兔激素性股骨头坏死血管内皮生长因子（VEGF）表达的影响，探讨其作用机制。
方法36只新西兰白兔随机分为三组：模型+克骨汤组（简称克骨汤组）12只、模型组12只、对照组12只。
在第4、8、12周分批处死实验动物，获取支配股骨头血运的主要血管（旋股内侧动脉、股静脉）及股骨头。
将其制成标本，采用原位杂交法测定VEGF含量，并行常规病理组织学检查。
结果第4周时，各组动脉内膜层、静脉内膜层中都有VEGF mRNA阳性染色，但各组间的差异无统计学意义（F分别=1.36、2.46，P均＞0.05）；第8周和第12周时，各组VEGF mRNA阳性染色的差异有统计学意义（F分别=14.58、11.35，P均＜0.05）；第8周时，与克骨汤组、对照组比较，模型组动脉内膜层、静脉内膜层的VEGF mRNA阳性染色减弱，差异均有统计学意义（t分别=12.13、15.23；19.36、18.25，P均＜0.05）；第12周时，克骨汤组与模型组、模型组与对照组比较，动脉内膜层、静脉内膜层VEGF mRNA阳性染色差异均有统计学意义（t分别=12.09、19.35；20.37、16.74，P均＜0.05），但克骨汤组与对照组比较，静脉内膜层VEGF mRNA阳性染色差异无统计学意义（t=2.59，P＞0.05）。
结论克骨汤可上调VEGF mRNA在血管内膜中的表达，使兔激素性股骨头坏死血管壁中主要促血管生长因子生理活性增强。
%Objective To investigate the effectof Kegu recipe on angiogenesis stimulating factors (VEGF mRNA)in the steroid-induced avascular ecrosis of the rabbit femoral head. Methods A total of 36 adult New Zealand rabbits were randomly divided into three groups equally: the control group, the model group, and the Kegu recipegroup. The experimental animals were partial executed at fourth, eighth, twelfth weeks so that to access the main blood vessels of the femoral head including the medial femoral circumfles artery, femoral medial femoral circumflex artery and femoral vein. The specimen used the in situ hybridization method to measure VEGF levels and underwent routine histologic examination. Results At fourth week, there was no statistical significance between three groups though all groups showed positive staining in VEGF mRNA of endarterium and veinintima(F=1.36,2.46,P>0.05). At eighth and twelfth week, the differences of VEGF mRNA positive staining in endarterium and vein intima were statistical significance (F=14.58,11.35,P<0.05). At eighth week, the VEGF mRNA positive staining in endarterium and vein intima of model group was significantly lower than Kegu recipe group and controlgroup(t=12.13,15.23,19.36,18.25,P<0.05). At twelfth week, the differences of the VEGF mRNA positive staining in endarterium and vein intima were statistical significance between model group and control group as well as between Kegu recipe group and modelgroup(t=12.09,19.35,20.37,16.74,P<0.05). The differences of the VEGF mRNA positive staining in vein intima between Kegu recipe group and control group had no statistical significances (t=2.59,P>0.05). Conclusions Kegu recipe could improve the presence of VEGF mRNA, which may boost the biological activity of the major factors for angiogenesis in the vascular wall of the steroid-induced necrosis of the femoral head.【期刊名称】《全科医学临床与教育》【年(卷),期】2013(000)004【总页数】4页(P373-376)【关键词】激素性股骨头坏死;克骨汤;血管内皮生长因子【作者】赵万军;杨武民;杨贺杰;夏志敏;周辉【作者单位】310007 浙江杭州，杭州市中医院骨伤科;310007 浙江杭州，杭州市中医院骨伤科;310007 浙江杭州，杭州市中医院骨伤科;310007 浙江杭州，杭州市中医院骨伤科;310007 浙江杭州，杭州市中医院骨伤科【正文语种】中文激素诱导的股骨头坏死模型动物处于高脂血症水平，高粘滞状态，血小板数量增多，血小板功能亢进，血液处于高凝状态，血管内皮功能受损。
丹酚酸B和高氧液对兔肢体缺血再灌注氧化应激损伤的影响涂泽松陈志维李逸群广东省佛山市中医院骨科(528000)[摘要】目的探讨高氧液和丹酚酸B对肢体缺血再灌注损伤的影响。
方法选用健康新西兰家兔24只，随机分为4组，在缺血前从耳缘静脉推注等量的生理盐水(A组)、高氧液(B组)、丹酚酸B(C 组)或高氧液加丹酚酸B(D组)，夹队股动、静脉，建立肢体缺血再灌注损伤模型，在缺血前和再灌注4h抽血检浏MDA, SOD，取排肠肌作病理检浏。
结果血清丙二醛浓度较前明显升高，高氧液和丹酚酸B可以抑制其升高(P < 0.01)，两者有协同作用(尸< 0.01);血清超氧化物歧化酶活性较前明显降低，高氧液和丹酚酸B可以抑制其降低(P<0.01)，两者有协同作用(尸<0.01)。
骨骼肌HE染色见:相对于对照组其他组骨骼肌损伤程度较轻，以联合用药组最轻。
结论高氧液和丹酚酸B能抑制肢体缺血再灌注氧化应激损伤，而且两者有协同作用。
【关键词】高氧液;丹酚酸B;缺血再灌注损伤Effects of salvianoli acid B and hyperoxic solution on experimental ischemia reperfusion坷ury TU Ze一song,CHEN Zhi一wei, !l Yi一，un . Department of Orthopedics，Foshan Traditional Chinese Medicine Hospital，Foshan 528000, China【Abstract) Objective Aim。
investigate the elf'eds of salvianoli acid B and hyperoxw solution on mpeamerual ischemia reperfusionir'uury of extremity. Method Tweet，一four healthy New Zealand rabbits~divided into four groups randomly. Equivalent 0. 9%ONaCI(group A )，hyperoxic solution (group B), salvianoli acid B (group C )，Salvianoli acid B and hyperoxic solution~injected through，吨e vein before ischemia. The femoral artery and vein~blocked and set tip the model of ischemia reperfusion injury of extremity. Beforeischernia and after 4 hours’mperfusion, the blood samples~taken to measure the levels of blood malondialdehyde ( MDA)supermidedismutuse(SOD).The gastrocnemius muscles~obseruetl by pathology test. Results Serum eruymatic activity of malondzaMehyde(MDA)~~obviously. Salvianoli acid B and hyperoxic solution can precwu the~了试P<0.01). They present~(P<0. 0l ). Serum Enzymatic activity of superoxide dismutase ( SOD) reduce obviously. Salvianoli acid B and hyperoxic solution can pre-vent the reduction of it ( P<0. 01) . They present interaction ( P<0.01).The test of HE staining show that other groups，lighter than thecontrol group at degree of skeletal muscle uyury. The groupD~the lightest of them. Conclusions Salvwrwli acid B and hyperoxw solu-tion~prevent the lipid peroxidation in ischernia reperfusion uyruy. They present interaction.【Key words I Hyperoxw solution; Salviartoli acid凡Ischemia reperfusion帅y.再灌注损伤是指组织器官在缺血一段时间后恢复血液灌实验当日进行充氧。
