BLOTHP幽门螺杆菌抗体分型

Advances in Clinical Medicine 临床医学进展, 2016, 6(4), 191-196Published Online December 2016 in Hans. /journal/acm /10.12677/acm.2016.64036文章引用: 柳晖, 白建文. 免疫印迹法测定健康体检人群幽门螺旋杆菌抗体及毒性分型结果分析[J]. 临床医学进展,Using Western Blod Assay to Analyze H. pylori Antibody and Toxicity Typing of Health Check-Up GroupHui Liu, Jianwen BaiDepartment of Physical Examination, Shanghai East Hospital, Tongji University School of Medicine, ShanghaiReceived: Nov. 15th , 2016; accepted: Dec. 16th , 2016; published: Dec. 19th, 2016Copyright © 2016 by authors and Hans Publishers Inc.This work is licensed under the Creative Commons Attribution International License (CC BY)./licenses/by/4.0/AbstractObjective: To analyze the infection types of Helicobacter pylori in different age groups from December 2014 to December 2012, i.e. antibodies and characteristics of toxicity distribution after infection. Me-thods: We use Western blotting method to detect and classify the immunophenotype of serum of physical examination population. Age is used as the statistical unit, results are tested and analyzed by χ2, and P < 0.05 means that the difference has statistical sense. Results: The positive rate of type II Hp infection of A urease or/and urease B for the check-up was 33.27% (5674/17,052), and age distribu-tion ranging from 30 to 69 enjoys the highest positive rate; the positive rate of type I Hp infection of cytotoxin A or/and vacuolate cytotoxin A was 10.58% (1804/17,052), and age ranging from 30 - 49 enjoys the highest positive rate. In different age groups of I and II, there exist differences (P < 0.05): low age group (18 - 30 years old) and old age group (>70 years) have lower possibilities to infect Hp. Conclusion: Among physical examination population, the rate of Hp infection and the immune typing results after infection, compared with clinical cases, both have some differences. Hp infection, as well as immune typing, exists special distribution characteristics among physical examination population.KeywordsHelicobacter pylori , Health Check-Up Group, Immune Phenotype, Immune Typing免疫印迹法测定健康体检人群幽门螺旋杆菌 抗体及毒性分型结果分析柳 晖，白建文柳晖，白建文同济大学医学院附属上海市东方医院体检科，上海收稿日期：2016年11月15日；录用日期：2016年12月16日；发布日期：2016年12月19日摘要目的：本文回顾性分析2012年12月至2014年12月健康体检人群不同年龄段幽门螺旋杆菌感染类型的情况，即感染后产生的抗体及毒性的分布特征。

系统医学 2023 年 2 月第 8 卷第 3期苏州地区幽门螺杆菌抗体分型在体检人群中的分布吉丽娟，钱王燕，耿春松上海交通大学医学院苏州九龙医院检验科，江苏苏州 215000[摘要] 目的 回顾性分析苏州地区幽门螺杆菌抗体分型在体检人群中的流行病学特征。

方法 选取2018年1—12月苏州市上海交通大学医学院苏州九龙医院的6 588名健康体检人群的临床资料，采用免疫印迹法定性检测人血清中多种幽门螺杆菌（helicobacter pylori, HP ）IgG 抗体，包括细胞毒素相关蛋白A （CagA ）、空泡毒素相关蛋白A （VacA ）、尿素酶亚单位A （UreA ）和尿素酶亚单位B （UreB ），观察体检人群幽门螺杆菌抗体分布情况。

结果 不同性别体检人群幽门螺杆菌感染率比较，差异无统计学意义（P >0.05）。

Ⅰ型Hp 抗体阳性率（29.6%）高于Ⅱ型Hp 抗体阳性率（26.7%）。

女性Ⅰ型Hp 抗体阳性率显著高于男性，差异有统计学意义（χ2=4.857，P =0.028）。

不同性别Ⅱ型Hp 抗体阳性率比较，差异无统计学意义（P >0.05）。

31~40岁之间男性体检人群幽门螺杆菌抗体分型分布阳性率（Ⅰ型26.12%，Ⅱ型26.95%）与同年龄女性体检人群（Ⅰ型30.23%，Ⅱ型24.23%）比较，差异有统计学意义（χ2=4.207，P =0.040）；41~50岁之间男性体检人群幽门螺杆菌抗体分型分布阳性率（Ⅰ型28.89%，Ⅱ型28.41%）与同年龄女性体检人群（Ⅰ型35.10%，Ⅱ型24.20%）比较，差异有统计学意义（χ2=7.531，P =0.006）。

结论 苏州地区体检人群幽门螺杆菌抗体阳性率较高，达56.30%，但不同性别之间无差异性；31~40岁与41~50岁两个年龄段之间女性Ⅰ型Hp 抗体阳性率高于男性，Ⅱ型Hp 抗体阳性率低于男性。

[关键词] 幽门螺杆菌；毒素相关蛋白A ；尿素酶；流行病学特征[中图分类号] R4 [文献标识码] A [文章编号] 2096-1782（2023）02(a)-0078-05Distribution of Helicobacter Pylori Antibody Typing in Physical Examina⁃tion Population in Suzhou AreaJI Lijuan, QIAN Wangyan, GENG ChunsongDepartment of Clinical Laboratory, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, Su⁃zhou, Jiangsu Province, 215000 China[Abstract] Objective To retrospectively analyze the epidemiological characteristics of Helicobacter pylori antibody typing in physical examination population in Suzhou area. Methods Clinical data of 6 588 healthy people from Suzhou Jiulong Hospital of Shanghai Jiao Tong University School of Medicine in Suzhou City from January 2018 to December 2018 were selected. Western blotting was used to qualitatively detect multiple helicobacter pylori (HP) IgG antibodies in human serum. Including cytotoxin-associated protein A (CagA), vacuolar toxin-associated protein A (VacA), urease subunit A (UreA) and urease subunit B (UreB), the distribution of Helicobacter pylori antibody in the physical exami⁃nation population was observed. Results There was no statistically significant difference in Helicobacter pylori infec⁃tion rate among different gender groups (P >0.05). The positive rate of type Ⅰ Hp antibody (29.6%) was higher than that of type Ⅱ Hp antibody (26.7%). The positive rate of Hp Ⅰ antibody in female was significantly higher than that in male, and the difference was statistically significant (χ2=4.857, P =0.028). There was no statistically significant dif⁃ference in the positive rate of Hp antibody type Ⅱ between different genders (P >0.05). The positive rate of Hp anti⁃body typing in males aged 31 to 40 (26.12% type Ⅰ, 26.95% type Ⅱ) was significantly higher than that in females DOI ：10.19368/ki.2096-1782.2023.03.078[作者简介] 吉丽娟（1989-），女，本科，主管技师，主要从事病原微生物致病机制与耐药性研究工作。

HP抗体免疫印迹法分型对胃肠道疾病的诊断价值摘要】目的：用免疫印迹法对幽门螺杆菌抗体（Helicobacter Pylori，Hp）分型（Ⅰ型和Ⅱ型）的结果进行比较分析。

方法：搜集我院100例被诊断为胃肠道不适(包括慢性胃炎，胃溃疡，胃癌等)患者和100例无症状的健康体检者血清，采用Hp分型检测试剂盒对幽门螺杆菌抗体Ⅰ型和Ⅱ型进行统计结果：被诊断为胃肠道不适患者HpⅠ型抗体阳性率明显高于健康体检者(P<0.01)，胃肠道疾病患者HPⅡ型抗体阳性率低于健康体检者阳性率（P<0.05) 结论：免疫印迹法可以作为检测幽门螺杆菌的初筛方法，并且HpⅠ型（CagA\VacA）比HpⅡ(UreB\UreA)型对胃肠道疾病的诊断和治疗具有更高的临床价值。

【关键词】免疫印迹法；幽门螺杆菌；胃肠道疾病【中图分类号】R573 【文献标识码】A 【文章编号】2095-1752（2016）01-0188-02The value of immunoblotting in typing Helicobacter Pylori antibody for theDiagnosis of Gastrointestinal DiseasesYuan Xuefei,HangJuan, Shao Yingchun, Yang Hu,Cheng Yunfei, Meng Yan, Ren Chuanlu*Depart. of Lab. Of No.100 hospital,Suzhou,215007,China【Abstract】Objective To analyze and compare the type(typeⅠand type Ⅱ) of Helicobacter pylori antibody(Hp) by immunoblotting method.Methods 100 patients diagnosed as gastrointestinal discomfort(chronic gastritis, gastric ulcer, gastric cancer and so on)and 100 asymptomatic healthy people were selceted,using the detection kit for typing Hp antibody of type Ⅰ and type Ⅱ.Results Diagnosed with gastrointestinal discomfort in patients with type Ⅰantibody positive rate is more higher than healthy subjects (P<0.01). The positive rate of type Ⅱantibody in patientswith gastrointestinal diseases is lower than in healthy persons(p<0.05) Conclusion Immunoblotting method can be used as a screening method in typing detection of Hp infection. Hp typeⅠ(CagA\VacA）has higher clinical value in the diagnosis and treatment of gastrointestinal diseases than type Ⅱ(UreB\UreA).【Key words】Immunoblotting; Helicobacter pylori; Gastrointestinal diseases 幽门螺杆菌是慢性活动性胃炎，消化性溃疡以及胃癌等胃肠道疾病的重要致病因子[1 -2]。

免疫印迹法检测幽门螺杆菌抗体及其分型的结果分析杨燕;单万水;韩红星;李小勇;李志明【期刊名称】《检验医学与临床》【年(卷),期】2013(10)A01【摘要】目的本文通过检测患者血清中幽门螺旋杆（Hp）抗体及分型，分析Hp 抗体分型在临床上的感染情况，为临床诊断与治疗提供依据。

方法随机选取该院门诊及住院患者476例，取空腹血用免疫印迹法检测Hp抗体谱，分析相应的Hp 细胞毒素相关蛋白（CagA）、空泡毒素（VacA）、尿素酶（UreaseA、B）在不同人群中的表达情况。

结果各年龄组对比年龄越大Hp感染率越高，疾病组对比胃炎，胃溃疡组Hp感染率最高，其他疾病，乙肝及体检患者感染率在40％～50％之间，差异无统计学意义。

结论应用免疫印迹法可一次性检测Hp抗体谱，进行HP抗体分型有利于临床对上消化道疾病病情的判断和治疗；免疫印迹法检测幽门螺杆菌抗体具有较高的敏感性和特异性。

方法简单，时间短，对临床治疗有重要指导意义。

【总页数】3页(P28-30)【作者】杨燕;单万水;韩红星;李小勇;李志明【作者单位】深圳市第三人民医院,广东深圳518000【正文语种】中文【中图分类】R725.7【相关文献】1.间接免疫荧光法检测抗核抗体与免疫印迹法检测抗核抗体谱结果分析 [J], 田巧2.免疫印迹法测定健康体检人群幽门螺旋杆菌抗体及毒性分型结果分析 [J], 柳晖;白建文;;3.间接免疫荧光法检测抗核抗体与免疫印迹法检测抗核抗体谱结果分析 [J], 田巧;4.886例体检者幽门螺杆菌抗体分型检测结果分析 [J], XU Jin-xia;LU Yun;LIU Lai;XU Zhong5.免疫印迹法检测幽门螺杆菌抗体结果分析 [J], 郑海玲;莫成锦因版权原因，仅展示原文概要，查看原文内容请购买。

886例体检者幽门螺杆菌抗体分型检测结果分析XU Jin-xia;LU Yun;LIU Lai;XU Zhong【期刊名称】《中国血液流变学杂志》【年(卷),期】2018(028)004【摘要】目的探讨幽门螺杆菌(HP)感染在健康体检者中的分布及流行状况.方法采用免疫印迹法同时检测血清HP四种抗体(CacA抗体、VacA抗体、UreA抗体、UreB 抗体)水平,计算其阳性率,用统计学方法比较各组差异.结果 886 例健康体检者HP总感染率为63.9%(566/886),其中,Ⅰ型322 例,占总感染率的56.9%(322/566),Ⅱ型244 例,占总感染率的43.1%(244/566).按照性别分组,男性共470 例,HP阳性291 例,感染率61.9%;女性共416 例,HP阳性275 例,感染率66.1%.不同性别组间感染率和分型进行χ2检验,HP感染率、Ⅰ型感染率和Ⅱ型感染率差异均无统计学意义(P＞0.05),按照年龄分布分为三组,＜40 岁组共144例,HP阳性76 例,感染率52.8%;40～59 岁组共525 例,HP阳性361例,感染率68.8%;≥60 岁组共217 例,HP阳性129 例,感染率59.4%.结果表明,随着年龄的增大,HP感染率先增后减,其中各个年龄段的Ⅰ型感染率均比Ⅱ型感染率高.对不同年龄组进行χ2检验,＜40 岁组与≥60 岁组的HP阳性率及Ⅰ型感染率与40～59 岁组间差异均有统计学意义(P＜0.05),而Ⅱ型感染率差异无统计学意义(P＞0.05).结论体检人群HP的Ⅰ型感染率在40～59 岁组达最高,且免疫分型在体检人群中存在特殊的分布特征.%Objective To investigate the distribution and prevalence of Helicobacter pylori (HP) infection in healthy people by physical examination. Methods The levels of four antibodies (CacA antibody, VacA antibody, UreA antibody, UreB antibody) of HP were detected by Westernblotting and the positive rate was calculated. Results The total infectionrate of HP was 63.9% (566/886) in 886 healthy persons, of which 322 cases were typeⅠ, accounting for 56.9% (322/566) of the total infection rate, and 244 cases of type Ⅱ, accounting for 43.1% (244/566) of the total infect ion rate. According to the sex group, there were 470 males and 291 HP positive cases, the infection rate was 61.9% (291/470), the positive rate of HP was 66.1% (275/416) in women and 416 cases in females. χ2 test showed that there was no significant difference in HP infection rate, typeⅠ infection rate and type Ⅱ infection rate among different sex groups (P＞0.05). According to the age distribution, 886 cases were divided into three groups. 144 cases were under 40 years old, the positive rate of HP was 52.8% (76/144). There were 525 cases in 40-59 age group and 361 cases in HP positive group, the infection rate was 68.8% (361/525). 217 cases wereover 60 years old, 129 cases were HP positive, the infection rate was 59.4% (129/217). The results showed that HP infection increased first and then decreased with age, and the infection rate of type Ⅰ was higher than thatof type Ⅱ in all age groups. χ2 test showed that there were significant differences in HP positive rate and type Ⅰ infection rate between thegroup of under 40 and over 60 years old and the group of 40-59 years old (P＜0.05), but there was no significant difference in type Ⅱ infection rate (P ＞0.05). Conclusion The infection rate of HP type Ⅰ was the highest in the 40-59 age group, and the immunophenotype had special distribution characteristics in the physical population.【总页数】3页(P467-469)【作者】XU Jin-xia;LU Yun;LIU Lai;XU Zhong【作者单位】Department of Clinical Laboratory, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu, 215001, China;Department of Clinical Laboratory, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu, 215001, China;Department of Clinical Laboratory, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu, 215001, China;Department of Clinical Laboratory, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu, 215001, China【正文语种】中文【中图分类】R446.5【相关文献】1.4929例健康体检人群幽门螺杆菌4种抗体检测结果分析 [J], 张玲;王雯娟;翁海斌;韦丽丽;余敏2.102例血清幽门螺杆菌-IgG抗体检测结果分析 [J], 刘家凤;骆敏;翁航爱;陈清容3.496名金融从业人员幽门螺杆菌抗体检测结果分析 [J], 李航;赵文敏;马春燕4.150例不同年龄人群血清幽门螺杆菌抗体检测结果分析 [J], 王朝晖[1];窦雁宁[2]5.2010-2013年丽水市中心医院幽门螺杆菌IgG抗体检测结果分析 [J], 徐燕;胡兰娟;郑颖因版权原因，仅展示原文概要，查看原文内容请购买。

２０２１年８月 第１５期影像学及诊断检验抗体分型检测在幽门螺杆菌感染诊断中的应用价值李春鸿，林养，吴春芳，杨康土湛江市第四人民医院，广东 湛江 524008【摘要】目的：探讨抗体分型检测在幽门螺杆菌感染诊断中的应用价值。

方法：选取健康体检者200名，使用免疫印迹法进行幽门螺杆菌抗体分型检测，根据抗体分型分为I型(98例)和II型(102例)，记录两组患者的幽门螺杆菌感染情况。

结果：①200名体检者中共发现幽门螺杆菌感染140例，幽门螺杆菌感染率为70.0%。

其中I型组感染84例，感染率为42.0%；感染抗体分型主要是细胞毒素、空泡毒素、尿素酶A、尿素酶B，均为阳性20例，占23.8%；细胞毒素、尿素酶B阳性10例，占11.9%；II型组感染56例，感染率为28.0%，尿素酶B为阳性，细胞毒素、空泡毒素、尿素酶A为阴性最为常见，10例，占17.9%，其次为尿素酶A为阳性、细胞毒素、空泡毒素、尿素酶B为阴性，3例，占5.4%，尿素酶A与尿素酶B阳性、细胞毒素、空泡毒素为阴性，3例，占5.4%。

②幽门螺杆菌抗体分型I型患者呼气实验阳性率52.4%，显著高于抗体分型II型患者呼气实验阳性率10.7%，P<0.05。

结论：抗体分型检测有助于诊断幽门螺杆菌感染，提高诊断水平，值得临床推广。

【关键词】抗体分型检测；幽门螺杆菌；感染[中图分类号]R57 [文献标识码]A [文章编号]2096-5249(2021)09-0196-02幽门螺杆菌是寄生在人体胃黏膜表层的病原菌，其不仅是慢性活动性胃炎、消化性溃疡等疾病的重要诱因，而且与胃癌的发生、发展也有直接关系[1]。

本文对抗体分型检测在幽门螺杆菌感染诊断中的应用价值进行了探讨，现报道如下：1 资料与方法1.1一般资料　选取2019年1月～2019年12月的健康体检者200名，其中，男性122例，女性78例，年龄在18～82岁之间，平均45.9±5.8岁。