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广西富硒区土壤耐硒菌株的分离及鉴定廖青;梁潘霞;邢颖;黄太庆;刘永贤;江泽普【摘要】[目的]从广西富硒区土壤中分离出多株耐硒菌株,为土壤硒资源开发利用提供参考.[方法]在广西主要富硒区永福、巴马、玉林寒山、桂平、藤县等地采集田间土样,利用稀释平板法并通过加硒培养对耐硒微生物进行分离、筛选.[结果]筛选得到8株耐硒能力较强的菌株,其在固体培养基中对硒的耐受浓度均在10000μg/mL以上.8株耐硒菌株中,YLB1-33耐硒能力最强,其在含硒量为29000μg/mL的固体培养基中仍能微弱生长.经16S rDNA序列分析和系统发育分析,鉴定结果表明,YLB1-6为蜡质芽孢杆菌、BMB2-1和TXB 1-8为短小芽孢杆菌、GPB2-5为苏云金芽孢杆菌、YLB 1-26和YLB1-33为地衣芽孢杆菌、YLB1-2和YFB1-8为粘质沙雷氏菌.[结论]耐硒菌株的发现对广西土壤硒资源利用、富硒农产品开发具有潜在的应用价值.%[Objective] To provide reference for Se resource exploitation of soil,many strains of Se-tolerance were isolated from Se-rich soil in Guangxi.[Method]The dilution spread plate method and Se-added culture method were used to screen the Se-tolerance strains from the soils which were sampled from the main Se-rich areas such as Yongfu,Bama,Yulin Hanshan,Guiping and Tengxi.[Result]8 strains with high Se-tolerance capacity were obtained.These strains could tolerate the Se concentration above 10000μ g/mL in solid medium.Among the 8 strains,YLB1-33 showed the highest Se-tolerance.It could still grow weakly in the solid medium with Se concentration 29000 μg/mL.Based on the sequencing of 16S rDNA and phylogenetic analysis,results showed that YLB1-6 was identified as Bacillus cereus,BMB2-1 and TXB1-8 wereidentified as Bacillus pumilus,GPB2-5 was identified as Bacillus thuringiensis,YLB1-26 and YLB1-33 were identified as Bacillus licheniformis,and YLB1-2 and YFB1-8 were identified as Serratia marcescens.[Conclusion]The finding of Se-tolerance strains had potential application value on promoting the utilization of Se soil resources and the development of Se-rich agricultural products in Guangxi.【期刊名称】《西南农业学报》【年(卷),期】2017(030)010【总页数】5页(P2303-2307)【关键词】富硒土壤;耐硒;菌株;鉴定;广西【作者】廖青;梁潘霞;邢颖;黄太庆;刘永贤;江泽普【作者单位】广西农业科学院农业资源与环境研究所,广西南宁530007;广西农业科学院农业资源与环境研究所,广西南宁530007;广西农业科学院农业资源与环境研究所,广西南宁530007;广西农业科学院农业资源与环境研究所,广西南宁530007;广西农业科学院农业资源与环境研究所,广西南宁530007;广西农业科学院农业资源与环境研究所,广西南宁530007【正文语种】中文【中图分类】S153.6【研究意义】硒(Se)是人体必需的15种微量元素之一，适度开发利用土壤硒资源，生产天然富硒农产品，对于满足人们日常补硒的需求尤为重要。
热带作物学报2021, 42(2): 519-526Chinese Journal of Tropical Crops甘蔗组培苗2种污染细菌的分离与鉴定刘红坚，李松，何为中，刘俊仙，刘丽敏，卢曼曼，游建华，黄诚华，林善海*广西农业科学院甘蔗研究所/中国农业科学院甘蔗研究中心/农业农村部广西甘蔗生物技术与遗传改良重点实验室，广西南宁 530007摘要：细菌污染是甘蔗组培苗生产上的一个主要问题。
为明确甘蔗组培中常出现的污染细菌的种类，本研究通过梯度稀释的方法分离污染细菌，结合生化特性、16S rRNA和phaC基因序列比对及系统发育分析鉴定其分类地位。
结果表明，共分离获得2种不同类型污染细菌，除了甘露醇，其余9个生化指标完全一致。
基因序列比对发现，2种类型分离物的种内2个基因序列完全一致，而种间16S rRNA序列只有2个碱基差异，种间phaC序列有23个碱基差异。
2个基因序列构建的系统发育树显示，2种细菌遗传距离较近，并均能形成独立的2个分支。
结合生化特征、序列比对及系统发育树，将2种污染细菌分别鉴定为巨大芽孢杆菌（Bacillus megaterium）和阿氏芽孢杆菌（B. aryabhattai），且首次报道阿氏芽孢杆菌为甘蔗组织培养中的污染细菌。
本研究揭示了巨大芽孢杆菌可能来源于甘蔗的内生菌，而阿氏芽孢杆菌来源于环境。
关键词：甘蔗；组培苗；污染细菌；芽孢杆菌；系统发育树中图分类号：S566.1 文献标识码：AIsolation and Identification of Two Bacteria Contaminated Sugarcane Tissue Culture SeedlingsLIU Hongjian, LI Song, HE Weizhong, LIU Junxian, LIU Limin, LU Manman, YOU Jianhua,HUANG Chenghua, LIN Shanhai*Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Sugarcane Research Center, Chinese Academy of Agri-cultural Sciences / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi 530007, ChinaAbstract: Contamination is a main issue in sugarcane tissue culture. The purpose of this study was to determine the contaminative bacteria frequently occurred during the production of sugarcane tissue culture. The gradient dilution method was used to isolate bacteria, and the toxonomic position was identified combining the biochemical, sequence alignment of 16S rDNA and phaC genes, phylogeomic analysis. The results showed that two type contaminative bacteria were obtained from the sugarcane tissue culture nutrient solution, and nine tested biochemical characters were the same between the two bacteria except mannitol utilization. Sequence alignment showed that intraspecific sequences of 16S rDNA and phaC genes were completely uniformity respectively in the two bacteria, but the difference of the two nu-cleotide bases in 16S rDNA and 23 in phaC sequences existed between the two bacteria. The phylogenic tree constructed based on 16S rDNA and phaC genes sequences respectively also indicated that the two bacteria were closest each other and clustered into two absolutely independent branches. Combining the biochemical characters, sequence alignment and phylogenetic analysis, the two contaminative bacteria were identified as Bacillus megaterium and B. aryabhattai, re-收稿日期 2020-02-18；修回日期 2020-04-07基金项目 广西创新驱动发展专项资金项目（桂科AA17202042-3）；广西农业科学院稳定资助团队项目（桂农科2018YT04）；广西农业科学院基本科研业务专项（桂农科2017YM05）。
广西野生杨梅种质资源初步调查与开发利用何新华;李峰;潘鸿;潘介春;黄桂香【摘要】通过对广西13个县野生杨梅资源的调查与研究,发现广西杨梅有3个种1个变型:杨梅、毛杨梅和青杨梅,其中青杨梅有1个变型--大叶青杨梅.分析和研究75份野生杨梅种的果实特性和经济价值,从中选出可在生产上应用的6个优良单株,并提出了开发利用广西杨梅资源的对策措施.【期刊名称】《种子》【年(卷),期】2007(026)002【总页数】3页(P64-66)【关键词】杨梅;种质资源;调查;开发利用【作者】何新华;李峰;潘鸿;潘介春;黄桂香【作者单位】广西大学农学院,南宁,530004;广西大学农学院,南宁,530004;广西大学农学院,南宁,530004;广西大学农学院,南宁,530004;广西大学农学院,南宁,530004【正文语种】中文【中图分类】S667.6;S602.4杨梅原产于我国南方，是我国著名的特产水果，在我国分布于东经97°～122°和北纬18°～33°，但经济栽培集中在东经103°以东和北纬31°以南地区，主要集中在东南沿海的浙江、江苏、福建、广东、江西、安徽、湖南、贵州等省，其中浙江省的栽培面积和产量最多［1］。
虽然广西杨梅经济栽培不多，但多样的生态环境造就了杨梅资源丰富，广西杨梅有3个种1变型［2］，即杨梅、毛杨梅、青杨梅(包括1个变型:大叶青杨梅)。
钱开胜等［3］对浔江上游野生杨梅资源进行了调查，发现野生杨梅资源十分丰富，主要为杨梅和青杨梅，并选育出了6个优良单株。
但针对广西全区野生杨梅资源情况的研究未见详细报道，为了进一步了解和掌握广西区内杨梅资源情况，开发利用本地优良杨梅资源，发展壮大广西杨梅产业，我们进行了本项研究，现将研究结果总结如下。
调查了广西北海市的合浦县、钦州市的灵山、玉林市的容县、南宁的良庆区、邕宁区、武鸣县和马山县、防城港市的上思县、桂林市的临桂县、灌阳县和龙胜县，河池的环江以及百色市的凌云县等地的野生杨梅资源，发现广西野生杨梅有杨梅科(Myricaceae)，杨梅属(Myrica)中的3个种1个变型，即杨梅(M．rubra Sieb et Zucc)、毛杨梅(M．esculenta Buch Ham)和青杨梅(M．adenophora Hance)，其中青杨梅有 1 个变型——大叶青杨梅，这些结果与已有的报道相同［2］。
收稿日期：2022-07-22基金项目：国家自然科学基金项目（31960307）；广西自然科学基金项目（2018GXNSFAA28110）；广西自筹经费林业科技项目（桂林科研［2022ZC ］第77号）作者简介：田湘（1974-），男，湖南汉寿人，高级工程师，主要从事林业资源经营与管理研究，（电话）150****1668（电子信箱）1412250937@qq.田湘，韩小美，黄平升，等.一株望天树根际高效解磷细菌解磷能力的研究［J ］.湖北农业科学，2023，62（9）：51-56．一株望天树根际高效解磷细菌解磷能力的研究田湘1，韩小美2，黄平升1，李万年2，杨钦潮1，杨梅2（1.广西南宁良凤江国家森林公园，南宁530004，2.广西大学林学院，南宁530004）摘要：以1株来源于广西南宁树木园望天树（Parashorea chinensis Wang Hsie.）根际土壤的高效解无机磷细菌P4［洋葱伯克霍尔德菌（Burkholderia cepacia ）］为材料，研究了解磷细菌P4在培养168h 内解磷能力和菌液pH 的动态变化情况，比较了菌株在不同温度、pH 、碳源、氮源、碳氮比（C/N ）和NaCl 浓度条件下的解磷能力，以期为解磷微生物在微生物菌肥生产上的开发和应用提供理论依据。
结果表明，在培养168h 内，菌株P4培养液中有效磷含量呈先升高后降低的趋势，pH 则呈先降低后升高的趋势，在培养120h 时菌液中有效磷含量最高，为552.87mg/L ，此时菌液pH 最低；相关性分析表明，菌株P4溶磷量与培养液pH 间存在极显著的负相关；菌株P4在培养温度为30℃、pH 为6.5、碳源为乳糖、氮源为草酸铵、C/N 为20∶1、NaCl 浓度为0.5%时解无机磷效果最好且生长状况良好，在后续微生物菌肥研制中具有较大潜力。
关键词：解磷细菌；解磷能力；根际；望天树（Parashorea chinensis Wang Hsie.）中图分类号：S154.39文献标识码：A文章编号：0439-8114（2023）09-0051-06DOI:10.14088/ki.issn0439-8114.2023.09.009开放科学（资源服务）标识码（OSID ）：Study on phosphate-solubilizing ability of a high efficiency phosphate-solubilizing bacteriumin rhizosphere of Parashorea chinensis Wang Hsie.TIAN Xiang 1，HAN Xiao-mei 2，HUANG Ping-sheng 1，LI Wan-nian 2，YANG Qin-chao 1，YANG Mei 2（1.Nanning Liangfengjiang National Forest Park ，Nanning 530004，China ；2.College of Forestry ，Guangxi University ，Nanning 530004，China ）Abstract ：A highly efficient inorganic phosphorus degrading bacterium P4（Burkholderia cepacia ）from the rhizosphere soil of the Parashorea chinensis Wang Hsie.in Nanning Arboretum in Guangxi was used as the research material ，the dynamic changes of phos⁃phate-solubilizing ability and pH of phosphate-solubilizing bacteria P4within 168h were studied ，and the phosphate-solubilizing ability of phosphorus bacteria P4under different temperature ，pH ，carbon source ，nitrogen source ，C/N and NaCl concentration was compared ，in order to provide a theoretical basis for the development and application of phosphate-solubilizing microorganisms in mi⁃crobial fertilizer production.The results showed that ，within 168h of culture ，the content of available phosphorus in the culture medi⁃um of strain P4increased first and then decreased ，while the pH of the culture medium decreased first and then increased.At 120h of culture ，the content of available phosphorus in the culture medium was the highest ，which was 552.87mg/L ，and the pH of the culture medium was the lowest.Correlation analysis showed that there was a highly significant negative correlation between the amount of phos⁃phorus dissolved by strain P4and the pH of the culture medium.The strain P4had the best effect of hydrolyzing inorganic phosphorus and grew well when the temperature was 30℃，pH was 6.5，carbon source was lactose ，nitrogen source was ammonium oxalate ，C/N was 20∶1and NaCl concentration was 0.5%.It had great potential in the follow-up development of microbial fertilizer.Key words ：phosphate-solubilizing bacteria ；phosphate-solubilizing ability ；rhizosphere ；Parashorea chinensis Wang Hsie.磷元素对植物生长具有重要影响，其在植物的光合作用、呼吸作用等方面均发挥着重要的作用，同时还能够促进植物根系的发育，对苗木初期的生长影响较大［1］。
