人(Human)胆红素(Bilirubin)ELISA试剂盒说明书

人B型脑钠肽（BNP）ELISA试剂盒使用说明

本试剂盒仅供研究使用。

检测范围： 96T

2μg/L -80μg/L

使用目的：

本试剂盒用于测定人血清、血浆及相关液体样本中B型脑钠肽（BNP）含量。

实验原理

本试剂盒应用双抗体夹心法测定标本中人B型脑钠肽（BNP）水平。用纯化的人B型脑钠肽（BNP）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入B型脑钠肽（BNP），再与HRP标记的B型脑钠肽（BNP）抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的B型脑钠肽（BNP）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中人B型脑钠肽（BNP）浓度。

试剂盒组成

1 30倍浓缩洗涤液 20ml×1瓶 7 终止液 6ml×1瓶

2 酶标试剂 6ml×1瓶 8 标准品（160μg/L） 0.5ml×1瓶

3 酶标包被板 12孔×8条 9 标准品稀释液 1.5ml×1瓶

4 样品稀释液 6ml×1瓶 10 说明书 1份

5 显色剂A液 6ml×1瓶 11 封板膜 2张

6 显色剂B液 6ml×1/瓶 12 密封袋 1个

标本要求

1．标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融

2．不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。

操作步骤

1. 标准品的稀释：本试剂盒提供原倍标准品一支，用户可按照下列图表在小试管中进行稀释。

80μg/L 5号标准品 150μl的原倍标准品加入150μl标准品稀释液

40μg/L 4号标准品 150μl的5号标准品加入150μl标准品稀释液

400-6721-600

1CatalogNumber

EK1349-48

EK1349-96

定量检测血清、血浆和细胞培养上清中的人Furin浓度。

本产品仅用于科学研究，非诊断试剂，不能用于临床诊断。

一、产品介绍

1.背景介绍

弗林蛋白酶(Furin)因其基因(fur)位于原癌基因fes/feps的上游而得名，是一种由794个氨基酸残基组成的Ⅰ型跨膜

蛋白，其主要结构包括信号肽、前体肽、枯草杆菌蛋白酶样催化结构域、P-结构域、富含半胱氨酸的结构域、疏水性的C-端跨膜结构域以及由58个残基组成的C端细胞质结构域。作为一种外泌途径中主要的蛋白质转化酶，Furin定位于高

尔基体外侧的网络中。弗林蛋白酶在胚胎发生和体内平衡中具有重要作用，并与癌症、神经退行性疾病和炭疽等各种病

理有关。此外，Furin不仅参与各种前体蛋白加工以及许多生长因子的成熟过程中，还涉及许多疾病，如由新冠病毒引

起的感染、骨相关疾病和肿瘤等，具有重要的生物学功能。

2.检测原理

本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Furin抗体预包被在高亲和力的酶标板上。酶标板孔

中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的Furin与固相抗体和检测抗体结合。洗涤去

除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。

颜色反应的深浅与样本中Furin的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570-630nm)测定吸光

度值。

3.试剂盒检测的局限

1)请在本试剂盒标示的有效期内使用。

2)试剂盒的试剂不能与其他批号的试剂或其他来源的试剂混合使用。

3)任何标准品稀释、操作人员、移液技术、洗涤技术、孵育温度、试剂盒保存时间的改变，都将影响结合反应。

4)

本试剂盒在设计上去除或降低了生物学样本中的一些内源性干扰因素，并非所有可能的影响因素都已经去除。HumanFurinELISAKit

（FOR RESEARCH USE ONLY. DO NOT USE IT IN CLINICAL DIAGNOSTICS !） Catalog No: E-EL-H6080 Size: 96T/48T/24T Human HBsAg(Hepatitis B surface antigen) ELISA Kit This manual must be read attentively and completely before using this product. If you have any problems, please contact our Technical Service Center for help (info in the header of each page). Phone: 240-252-7368(USA) 240-252-7376(USA) Email: *************************** Website: Please refer to specific expiry date from label outside of box. Please kindly provide us with the lot number (on the outside of the box) of the kit for more efficient service. Intended use This ELISA kit applies to the in vitro quantitative determination of Human HBsAg concentrations in serum and plasma, please inquir if your samples are other biological fluids. . Specification ●Sensitivity: 0.19ng/mL. ●Detection Range: 0.31-20ng/mL. ●Specificity: This kit recognizes Human HBsAg in samples. No significant cross-reactivity or interference between Human HBsAg and analogues was observed. ●Repeatability: Coefficient of variation is < 10%. Test principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HBsAg. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HBsAg and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HBsAg, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human HBsAg. You can calculate the concentration of Human HBsAg in the samples by comparing the OD of the samples to the standard curve. Kit components & Storage An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the following conditions once the kit is received. Item Specifications Storage Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips -20℃, 6 months Reference Standard 96T: 2 vials 48T: 1 vial 24T: 1 vial Concentrated Biotinylated Detection Ab(100×) 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL 24T: 1 vial, 60 μL Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL 24T: 1 vial, 60 μL -20℃(Protect from light), 6 months Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃, 6 months Biotinylated Detection Ab Diluent 1 vial, 14 mL HRP Conjugate Diluent 1 vial, 14 mL Concentrated Wash Buffer(25×) 1 vial, 30 mL Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light) Stop Solution 1 vial, 10 mL 2-8℃ Plate Sealer 5 pieces Product Description 1 copy Certificate of Analysis 1 copy Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s). Other supplies required Microplate reader with 450nm wavelength filter High-precision transfer pipette, EP tubes and disposable pipette tips Incubator capable of maintaining 37℃ Deionized or distilled water Absorbent paper Loading slotNote 1. Please wear lab coats, eye protection and latex gloves for protection. Please perform the experiment following the national security protocols of biological laboratories, especially when detecting blood samples or other bodily fluids. 2. A freshly opened ELISA plate may appear a water-like substance, which is normal and will not have any impact on the experimental results. Return the unused wells to the foil pouch provided in the kit, store it according to the conditions suggested in the above table. 3. Do not reuse the reconstituted standard, biotinylated detection Ab working solution, concentrated HRP conjugate working solution. The unspent undiluted concentrated biotinylated detection Ab (100×) and other stock solutions should be stored according to the storage conditions in the above table. 4. The microplate reader should be able to be installed with a filter that can detect the wave length at 450±10 nm. The optical density should be within 0-3.5. Follow the Instructions of the Microplate Reader for set-up and preheat it for 15 min before OD measurement. 5. Do not mix or use components with those from other lots. 6. Change pipette tips in between adding of each standard level, between sample adding and between reagent adding. Also, use separate reservoirs for each reagent. Sample collection (More detailed information please view our website: /List- detail-253.html) Serum: Allow samples to clot for 2 hours at room temperature or overnight at 2-8℃before centrifugation for 20 min at 1000×g at 2-8℃. Collect the supernatant to carry out the assay. Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 min at 1000×g at 2-8℃ within 30 min of collection. Collect the supernatant to carry out the assay. Tissue homogenates: It is recommended to get detailed references from the literature before analyzing different tissue types. For general information, hemolyzed blood may affect the results, so the tissues should be minced into small pieces and rinsed in ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then homogenized in PBS (tissue weight (g): PBS (mL) volume=1:9) with a glass homogenizer on ice. To further break down the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then centrifuged for 5-10 min at 5000×g at 2-8℃ to get the supernatant.

人抵抗素（resistin）酶联免疫分析试剂盒使用说明书

本试剂盒仅供研究使用

检测范围：0.32ng/ml–20ng/ml

最低检测限：0.08ng/ml

特异性：本试剂盒可同时检测天然或重组的resistin，且与其他相关蛋白无交叉反应。

有效期：6个月

预期应用：ELISA法定量测定人血清、血浆、细胞培养上清或其它相关生物液体中resistin

含量。

说明

1．试剂盒保存：-20℃（较长时间不用时）；2-8℃（频繁使用时）。

2．浓洗涤液低温保存会有盐析出，稀释时可在水浴中加温助溶。

3．中、英文说明书可能会有不一致之处，请以英文说明书为准。

4．刚开启的酶联板孔中可能会含有少许水样物质，此为正常现象，不会对实验结果造成任

何影响。

实验原理

用纯化的抗体包被微孔板，制成固相载体，往包被抗resistin抗体的微孔中依次加入标本或

标准品、生物素化的抗resistin抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显

色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的

深浅和样品中的resistin呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算

样品浓度。

试剂盒组成及试剂配制

1.酶联板（Assayplate）：一块（96孔）。

2.标准品（Standard）：2瓶（冻干品）。

3.样品稀释液（SampleDiluent）：1×20ml/瓶。4.生物素标记抗体稀释液（Biotin-antibodyDiluent）：1×10ml/瓶。

5.辣根过氧化物酶标记亲和素稀释液（HRP-avidinDiluent）：1×10ml/瓶。

6.生物素标记抗体（Biotin-antibody）：1×120μl/瓶（1：100）

7.辣根过氧化物酶标记亲和素（HRP-avidin）：1×120μl/瓶（1：100）

8.底物溶液（TMBSubstrate）：1×10ml/瓶。

9.浓洗涤液（WashBuffer）：1×20ml/瓶，使用时每瓶用蒸馏水稀释25倍。