苯酚生物降解菌的筛选

苯酚降解菌的筛选及降解性能研究王启明;彭仁;陈文静;杜芸芸;杨贵娟;简敏菲【期刊名称】《江西师范大学学报（自然科学版）》【年(卷),期】2012(036)003【摘要】从腐烂的树木枯枝和污染的淤泥中分离出苯酚的高效降解菌.通过对它们的形态和16S rDNA分析,筛选到的新菌株分别命名为Acinetobactersp.SD1,Pseudomonas sp.SD2和Rhodococcussp.SD3.在筛选到的3株菌中,Rhodococcus sp.SD3的苯酚降解性能最好,该菌株在72 h内几乎能将浓度为1.0 g/L的苯酚完全降解.%Phenol-degrading microbes were isolated from rotting wood and polluted sludge. These strains were identi-fied as Acinetobacter sp. SD1, Pseudomonas sp. SD2 and Rhodococcus sp. SD3 according to their morphological feature and 16S rDNA sequence, respectively.The phenol-degrading performance of Rhodococcus sp. SD3 strain was best among three strains, which almost completely degraded 1.0 g/L phenol in 72 hours.【总页数】4页(P317-320)【作者】王启明;彭仁;陈文静;杜芸芸;杨贵娟;简敏菲【作者单位】江西师范大学生命科学学院,江西南昌330022;江西师范大学生命科学学院,江西南昌330022;江西师范大学生命科学学院,江西南昌330022;江西师范大学生命科学学院,江西南昌330022;江西师范大学生命科学学院,江西南昌330022;江西师范大学生命科学学院,江西南昌330022【正文语种】中文【中图分类】X172【相关文献】1.高效苯酚降解菌的分离及降解性能的研究 [J], 赵宇虹;王月;兰莹;张跃华;赵坤宇2.苯酚降解菌的筛选及其降解性能研究 [J], 张雯;苏静静;曹丽琴3.沙雷氏苯酚降解菌固定化条件优化及降解性能研究 [J], 王春荣;高振凤;王建兵;程芳琳;常莎4.一株苯酚降解菌的筛选及其降解性能研究 [J], 付玲玲5.一株高效苯酚降解菌的筛选及降解性能考查 [J], 石瑞文;哈妮;郭楠;张晓琴;关海滨因版权原因，仅展示原文概要，查看原文内容请购买。

高效苯酚降解菌的分离及降解性能的研究引言石油、化工、煤气、焦化及酚类等生产厂排放的废水当中含有大量的苯酚[1]。

未经净化的含酚废水可导致水源被污染，致使鱼类死亡，危害农作物，最终威胁人类的健康。

许多国家将苯酚列为重要的污染物之一。

目前，国内外处理含酚废水的方法主要有物理法、化学法、微生物法及各种结合法[2]。

其中微生物法主要利用微生物的代谢活动去除废水中的有毒物，处理方法无2次污染且安全、经济。

目前，已鉴定具有降解苯酚能力的微生物主要有假单胞菌（Pseudonomonas.sp）[3]、芽孢杆菌（Bacillus.sp）[4]、酵母菌（Yeast trichosporon）[5]、根瘤菌（Rhizobia）[6]、醋酸钙不动杆菌（A. calcoaceticus）[7]等，降酚菌株多存在于酚类污染物企业排放的废水、污泥和被废水污染的土壤中[8]。

本课题拟从被苯酚废水污染的污泥中进行菌株筛选，得到耐酚菌后在以苯酚为唯一碳源的无机盐培养上筛选降酚菌株，进一步测定苯酚降解的影响因素。

对特定菌株降解含酚废水的应用价值进行研究。

1 实验材料和方法1.1 菌株来源采集原黑龙江省佳木斯东郊黑龙农药化工集团废弃排污口处污泥进行菌株筛选。

1.2 培养基基础培养基：NaCl 5.0g/L，蛋白胨10g/L，琼脂15～20g/L，酵母浸膏5.0g/L，调节pH为7.0。

以苯酚为唯一碳源的无机盐培养基：CaCl2 0.1 g/L ，FeSO4.7H2O 0.01 g/L，K2HPO4 0.5g/L，MnSO4.7H2O 0.05 g/L，NaCl 0.2 g/L，KH2PO4 0.5g/L，MgSO4.H2O 0.01 g/L，NH4NO3 1.0 g/L苯酚按实验需要量添加，调节pH为7.0 [8]。

富集培养基：葡萄糖10.0g/L，营养琼脂33.0g/L，酵母浸粉10.0g/L，调节pH为7.5。

1.3 研究内容与方法1.3.1 菌株和的驯化和分离在超净工作台中，将10mL含0.1g/L苯酚的基础培养基倒入培养皿，取10 g污泥加90mL蒸馏水搅拌15min，静置5min后取上层清液为菌原液[8]。

高效苯酚降解菌 PDB1的筛选及降解特性研究王图锦;潘瑾;刘雪莲【期刊名称】《科学技术与工程》【年(卷),期】2017(017)002【摘要】A bacterial strain which could degrade phenol with high efficiency was isolated from the polluted soils in a coke plant.The strain was named PDB1 and was identified as the genus Acinetobacter sp with 16S rRNA gene sequence .Biodegradation was investigated in details with phenol as the sole carbon and energy source .The results indicated that PDB1 could utilize phenol as a sole source of carbon and could degrade less than 1 200 mg/L phenol completely in 60 h, but higher phenol concentrations had an inhibitory effect .The process of phenol degradation was investigated using Haldane kinetic model .Phenol degradation kinetic studies indicated that the degradation fol-lowed Haldane ’ s model .The optimal concentration of initial phenol was 144.56 mg/L.The rate of phenol degra-dation increased with increasing initial phenol concentrations up to 144.56 mg/L.Further increase in initial phenol concentrations caused decrease in the rate of phenol degradation because of substrate inhibition .The optimal condi-tions for biodegradation of phenol were at 25~40 ℃,pH 7.0~8.0 and 0~5%NaCl.%从一个焦化厂受污染土壤中分离获得一株具有高效降解苯酚能力的不动杆菌属细菌PDB1，以苯酚为唯一碳源和能源对该菌株降酚特性进行研究。

酚类降解菌的筛选及作用效果的研究发布时间：2021-07-01T15:36:18.710Z 来源：《科学与技术》2021年7期作者：王其辉郝景雯曹琳琳[导读] 针对含酚工业废水，采用原位分离技术从其生物系统中分离得到好氧菌7株，兼氧菌4株，并利用16SrDNA测序方法进行了鉴定王其辉郝景雯曹琳琳天津市工业微生物企业重点实验室针对含酚工业废水，采用原位分离技术从其生物系统中分离得到好氧菌7株，兼氧菌4株，并利用16SrDNA测序方法进行了鉴定，经进一步筛选确定由蜡样芽胞杆菌、腐败希瓦氏菌等4株菌复配成好氧菌剂，贝莱斯芽孢杆菌、短小芽孢杆菌等3株菌组成兼氧菌剂。

以预处理后的含酚废水为目标，采用48小时酸化水解，72小时好氧曝气处理后，经菌剂降解性能的验证实验，结果表明：所研制的复配菌剂与原位样直接增值的培养液，无论是废水COD降解率，还是酚类物质降解率水平相当，说明研制的菌剂可以强化活性污泥。

关键字：原位分离含酚废水强化污泥高效降解Seven strains of aerobic bacteria and four strains of facultative bacteria were isolated from the biological system of phenol containing industrial wastewater by in-situ separation technology, and were identified by 16S rDNA sequencing method. After further screening, it was determined that four strains of Bacillus cereus, Shigella putrefaciens and three strains of Bacillus belies and Bacillus pumilus were combined to form an aerobic agent. After 48 hours of acidification and hydrolysis, 72 hours of aerobic aeration treatment, the degradation performance of the microbial agent was verified. The results showed that the degradation rate of COD and phenols in the wastewater of the compound microbial agent was equivalent to that of the in-situ sample, indicating that the developed microbial agent could strengthen the activated sludge.keyword: In situ separation，Phenolic wastewater，Enhanced sludge，Efficient degradation高效降解酚类物质微生物菌种的筛选是生物降解含酚工业废水的技术核心，原位分离技术是一种快速分离和筛选目标微生物的有效途径，由于长期自然驯化的原因，原位分离得到的微生物具有环境适应能力强、共生关系稳定和底物降解针对性好等特点，是一种值得推广的有效方法。