AgeI限制性内切酶使用说明书

H om e ›P roducts ›AgeIAgeIProduct Information FAQs Protocols Other Tools & Resources Related ProductsDescriptionProperties and UsageQuality Control This enzyme has transitioned to an improved new buffer system. Visit for further details.The new and current Double Digest Finder and current Activity/Performance Chart for the CutSmart buffer system are available. The previous version of the Double Digest Finder , as well as the previous Version of Activity/Performance Chart that use the former buffer system, are still available for your convenience.AgeI has High Fidelity (HF) and RE-Mix Master Mix versions available.IsoschizomersCatalog #Size Concentration Price Qty R0552S300 units 5,000 units/ml $67.001R0552L 1,500 units 5,000 units/ml $268.001Categories: Restriction Endonucleases: AApplications: Restriction Enzyme DigestionDescriptionReaction Conditions1X NEBuffer 1.1Incubate at 37°C1X NEBuffer 1.1:10 mM Bis-Tris-Propane-HCl10 mM MgCl2100 μg/ml BSApH 7 @ 25°CActivity in NEBuffersNEBuffer 1.1: 100%NEBuffer 2.1: 75%NEBuffer 3.1: 25%CutSmart™ Buffer: 75%Diluent CompatibilityDiluent CStorage Temperature-20°CStorage Conditions10 mM Tris-HCl250 mM NaCl1 mM DTT0.1 mM EDTA200 μg/ml BSA50% Glycerol0.15% Triton® X-100pH 7.4 @ 25°CHeat Inactivation65°C for 20 minMethylation Sensitivitydam methylation: Not Sensitiv edcm methylation: Not Sensitiv eCpG Methylation: BlockedActivity at Temperature@37°C: 100%Quality ControlQuality Control AssaysThe following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Indiv idual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Blue-White Screening (Terminal Integrity):A sample of DNA v ector linearized with a 10-fold excess of a restriction endonuclease, religated and transformed into an E. coli strain expressing the LacZ beta fragment gene results in less than 1% white colonies.Exonuclease Activity (Radioactivity Release):The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activ ity is determined by the % release of radioactiv e nucleotides.Ligation and Recutting (Terminal Integrity):After an ov er-digestion of DNA with a restriction endonuclease the percentage of the DNA fragments ligated with T4 DNA ligase andthe percentage that can be recut are determined by agarose gel electrophoresis.Non-Specific DNase Activity (16 hour):The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.Notes1. Star activ ity may result from extended digestion.Legal and DisclaimersPatentsNew England Biolabs, Inc.U.S. Patent No. 7,517,671Supporting DocumentsCertificate of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an indiv idual lot. The following file naming structure is used to name these document files: [Product Name]COA.R0552SLCoA-Lot0431302Material Safety DatasheetsThe following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name]MSDS. For international v ersions please contact us at info@ .AgeI MSDSNEBuffer 1.1 MSDSDatacardsThe Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@ or fill out the Technical Support Form for appropriate document.R0552Datasheet-Lot0431205R0552Datasheet-Lot04312091. What factors inhibit AgeI?2. Are extended digests of AgeI recommended?3. What is the reaction temperature of AgeI?4. My enzyme is no longer Time-Sav er™ qualified. What happened?5. What effect does BSA hav e on the performance of NEB’s restriction enzymes when included in the new buffers?6. How can I access the old NEBuffer Activ ity Chart?7. How can I access the old Double Digest Finder?1. Optimizing Restriction Endonuclease ReactionsSelection Tools Usage Guidelines & TipsTroubleshooting Guides Interactiv e ToolsSelection ToolsAlphabetized List of Recognition SpecificitiesCompatible Cohesiv e EndsCross Index of Recognition SequencesDam-Dcm and CpG MethylationFrequencies of Restriction SitesIsoschizomersRecleav able Filled-in 5' Ov erhangsTime-Sav er™ Qualified EnzymesWhy Choose Recombinant Enzymes?Usage Guidelines & TipsActiv ity at 37°C for Restriction Enzymes with Alternate Incubation TemperaturesActiv ity of Restriction Enzymes in a Taq or Phusion® PCR MixActiv ity of Restriction Enzymes in a Vent R® PCR MixCleav age Close to the End of DNA FragmentsCleav age of Supercoiled DNADigestion of Agarose-Embedded DNA: Info for Specific EnzymesDouble DigestsEffects of CpG Methylation on Restriction Enzyme Cleav ageHeat Inactiv ationMegabase MappingNEBuffer Activ ity/Performance Chart with Restriction Enzymes Optimizing Restriction Endonuclease Reactions Restriction Endonucleases - Surv iv al in a Reaction Restriction Enzyme Diluent Buffer CompatibilitySingle Letter CodesStar Activ ityTraditional Cloning Quick GuideTroubleshooting GuidesRestriction Enzyme Troubleshooting GuideInteractive ToolsDNA Sequences and Maps ToolDouble Digest FinderEnzyme FinderCompanion ProductsAgeI-HF™AgeI-HF® RE-Mix®Materials Sold SeparatelyNEBuffer 1.1。