(完整版)常用限制性内切酶酶切位点汇总,推荐文档

Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点与大家共享（非原创）。

限制性内切酶酶切位点汇AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsgI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsaI识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TliI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

限制性内切酶酶切位点汇总限制性内切酶（Restriction Endonuclease）是一类存在于细菌体内的酶，它能够识别特定的酶切位点，并在该位点上切割DNA链。

限制性内切酶起源于细菌，原本作为细菌对抗噬菌体感染的防御机制，但现在被广泛应用于分子生物学和基因工程领域。

限制性内切酶的分类和命名依据它们发现的第一个类型的噬菌体。

如EcoRI是从大肠杆菌中分离出的内切酶，与T4噬菌体相关；HindIII是与T4噬菌体有关的内切酶等。

酶名中的缩写首字母通常是酶切位点的首字母，比如EcoRI是指E.coli的RY粘性末端的切点。

现在限制性内切酶已被发现有超过3000多个类型。

下面是一些常见的限制性内切酶的切割位点汇总：1. EcoRI：切割位点为G↓AATTC（↓表示切割位点），产生的切割后的两个DNA片段是G-AATTC和CTTAA-G。

2. HindIII：切割位点为A↓AGCTT，产生的切割后的两个DNA片段是A-AGCTT和TTCGA-A。

3. SmaI：切割位点为CCC↓GGG，产生的切割后的两个DNA片段是CCC-GGG和GGG-CCC。

4. BamHI：切割位点为G↓GATCC，产生的切割后的两个DNA片段是G-GATCC和CCTAG-G。

5. XhoI：切割位点为C↓TCGAG，产生的切割后的两个DNA片段是C-TCGAG和GAGCT-C。

6. NotI：切割位点为GC×GGCCGC，产生的切割后的两个DNA片段是GC-GGCCGC和CGC-GC。

7. EcoRV：切割位点为GAT↓ATC，产生的切割后的两个DNA片段是GAT-ATC和TAC-TAG。

8. KpnI：切割位点为GGTAC↓C，产生的切割后的两个DNA片段是GGTAC-C和CCATG-G。

9. SalI：切割位点为G↓TCGAC，产生的切割后的两个DNA片段是G-TCGAC和CAGCT-G。

10. PstI：切割位点为CTGCA↓G，产生的切割后的两个DNA片段是CTGC-AG和GACG-T。

常用限制性内切酶酶切位点总结————————————————————————————————作者：————————————————————————————————日期：Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点doc版BsgI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点doc版BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点doc版EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

限制性内切酶酶切位点汇AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsgI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsaI识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TliI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点与大家共享（非原创）。

ApaI识别位点Acc65I识别位点ApaLI识别位点AccI识别位点ApeKI识别位点AciI识别位点ApoI识别位点AclI识别位点AscI识别位点AcuI识别位点AseI识别位点AfeI识别位点AsiSI识别位点AflII识别位点AvaI识别位点AflIII识别位点AvaII识别位点AgeI识别位点AvrII识别位点AhdI识别位点BaeI识别位点AleI识别位点BamHI识别位点AluI识别位点BanI识别位点AlwI识别位点BanII识别位点AlwNI识别位点BmrI识别位点BbvCI识别位点BmtI识别位点BbvI识别位点BpmI识别位点BccI识别位点Bpu10I识别位点BceAI识别位点BpuEI识别位点BcgI识别位点BsaAI识别位点BciVI识别位点BsaBI识别位点BclI识别位点BsaHI识别位点BfaI识别位点BsaI识别位点BfuAI识别位点BsaJI识别位点BglI识别位点BsaWI识别位点BglII识别位点BsaXI识别位点BlpI识别位点BseRI识别位点Bme1580I识别位点BseYI识别位点BmgBI识别位点BspMI识别位点BsiEI识别位点BspQI识别位点BsiHKAI识别位点BsrBI识别位点BsiWI识别位点BsrDI识别位点BslI识别位点BsrFI识别位点BsmAI识别位点BsrGI识别位点BsmBI识别位点BsrI识别位点BsmFI识别位点BssHII识别位点BsmI识别位点BssKI识别位点BsoBI识别位点BssSI识别位点Bsp1286I识别位点BstAPI识别位点BspCNI识别位点BstBI识别位点BspDI识别位点BstEII识别位点BspEI识别位点BstNI识别位点BspHI识别位点DdeI识别位点BstXI识别位点DpnI识别位点BstYI识别位点BstZ17I识别位点DpnII识别位点Bsu36I识别位点DraI识别位点BtgI识别位点DraIII识别位点BtgZI识别位点DrdI识别位点BtsCI识别位点EaeI识别位点BtsI识别位点EaeI识别位点Cac8I识别位点EagI识别位点ClaI识别位点EarI识别位点CspCI识别位点EciI识别位点CviAII识别位点EcoNI识别位点CviKI-1识别位点EcoO109I识别位点CviQI识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

专业资料分享A系列Aatll识别位点…G ACGT匕…一丁3：.. CJGC AG,, .5^Acc65l识别位点5…GFMK AC …3，3一. CA KMJG. . 5JAccl识别位点5…Gl\lK AC …丁3J. . CA KMJG. . 5JAcil识别位点5 …dtGC …3’ 宙…GG空…莎Acll识别位点5 …A A\；GTT .33\ .. T TG* A (X)Acul识别位点5；. CTG A AG(N). \ . . 3J 3d.. ,GACTTC(N)U A... 5J Afel识别位点5\.. AGC^CT..,3,3 …TCGJCGA..,5JAflll 识别位点5 …(fTT A AG…3，3：…G A A T T& …5"Afllll 识别位点5；.. ^CRYGT , ,3 3：…TGYRqA. . ,5Agel识别位点5P. . /fCCGGT , . .3J3；. . TGGCC^A. , .5^ Ahdl识别位点5P. G ACNN F/N NGTC... 3P 3- CTGNI^NNNCAG^.S^ Alel识别位点5；. .CACNt/NNGTG 3' 3. ..GTGNI^NNCAC, .. h Alul识别位点5 . .. A(jfcT….3P 3…TQSA-SAlwl识别位点5 r,GGATC(N)7「3"3. .. CCTAGIN)^..^AlwNl识别位点5：. CAGNNN T CTG..3F 3；. GTC^NNNG AC.. .5*Apal识别位点5 .., GGGCtft. ..3P3\., C^CCGGG .ApaLl识别位点5\.. (^TGCAC.. ,3J3 .. CACGTjGApeKl识别位点5P. . . t^CWGC., .3r3\. CGWCjp., .5^Apol识别位点5；,. FLATTY,, ,3J3 , _ Y TTA AJl. T.5"Ascl识别位点5：., GG^bGCGCC, .33 CCGCGCJ J G, .5JAsel识别位点5 . B 4A T't A A T t. , 3J3 . .. T A A TJ A .. 5JAsiSl识别位点5...GCGAl1SGC r. 3r3...CGCJAGCG . .5Aval识别位点可…C TV CGRG…3」3：..GRGCYJ>..hAvall识别位点5：…dfewCC,.,3r3；., CCWG^.. 5JAvrll识别位点5... C T CT AGG .3’…GGATCf…5」Bael识别位点5；^(NJACIN^GTAYCCNjJ 33.^^NJTGjN^CATRGtN)^ .5^识别位点吕,"・G E AT CC…3’BamHl专业资料分享3’…C C T A Gp -.. 5"Banl识别位点吕…G^YRCC^ 33 …CCRYQp^ .5JBanII识别位点5：.. GRGC Y^t …3",C A Y C G R G . ,,Bbsl识别位点5. .,GAAGAC (N)/ . .3r3J. .. 0 T T C T G 减丄…S' BbvCI识别位点5 …C(T T CAGC.”.3*3 …GGAGT A CG.,S JBbvl识别位点5 …GCAGCHN}；…3* 計…CQTCG(N)iar .5^Bccl识别位点5P. . CCATC(N)/,. 33\ , .GGTAG(N). -5rkBceAl识别位点5... ACGGCtNjJ. ,3^3：.. TGCCG(N)F「5*Bcgl识别位点5 . T4N)CGA(NK TGC(N)J . .3J 3；.^(N)GCT(N)；ACG{N)^.b5^BciVl识别位点5. r,GT ATCC (N)J .3F3. ..C AT AGG (N}t^ ..5PBcll识别位点5... T'feATC A,- 3J3：…ACT AGJ., 5JBfal识别位点5：.. (?T AG . .3r苗…GA.C…hBfuAl识别位点5；. . ACCTGC(N)J 3’3. ..TGG ACG(N)^, .5^Bgll识别位点5；. GCCNNNN^JGGC., 3P3 . . CGGr^hlNNNCCG. , 5*Bglll识别位点莎…A T G T…3’3：”. TCTAt^A.. 5rBlpl识别位点5•…G(7TN AGO., -3J3；., CGAN T；CG , .5^Bme1580l识别位点5 . . GKGCWit …才3 . . Q^MCGKG ,5JBmgBl识别位点h…C AC令TC…孑3 . . .GTGJ： AG. . 5*Bmrl识别位点5... ACTGGG(N) T r.3r3... TGACCC(N)4r..5-Bmtl识别位点h…GCTA(?b …3’3\, C/3 ATCG …5」Bpml识别位点5 .… CTGGAG(N)1P T 3r3... GACCTC(N)14j...5rBpulOI识别位点5’…CCT T N AGC …*33\., GGAN^CG .. 5BpuEl识别位点5；.. CT TG AG(N)r T,.3'3\.. GAACTC(N)…A...S'BsaAl识别位点5；.. YAC'GTR . .3^BsaBl识别位点5： ..GATNN^NATC , .3^3：, ,CTANN/1NTAG,. . 5^BsaHl识别位点5P. . .GFitG YC . . ,3^3...C YGC^RG.. .5JBsal识别位点5... GGTCTC (N)/. .3^3... C C A G A G (N)^. . . 5' BsaJl识别位点5 c'b NNGG.. 3J3； GGNNC/3,. 5BsaWl识别位点5 . ., w'bCGGW, 3J3•…WGGC即…hBsaXl识别位点5 . ^NjACfN^CTCCtN),；. 3-3r ..(N)TG(N)r GAGG(N). .，& 丄p ABseRl识别位点5…GAG GAG的「…3"3... CTCCTC{N)-A….5P BseYl识别位点5\, CV： CAGC.,. 3J CT…GGGTQ3…可Bsgl识别位点5；.. GTGCAG^N^T. 3J3 ….CACGTC{Nj i4i.. . 5JBsiEI识别位点5, . , CGRY'fcG,, 3r3 . .. GC A YRGC . ,5"BsiHKAI识别位点5；…GWGCW七■…J3：..C^WCGWG.. .5BsiWI识别位点5.C'feT ACG, . 3r 3：…GC A TGJS. 5’Bsll识别位点5' . , CCNNNN I^INGG. , .3r 3；., GGN\NNNNNCC.. ,5J BsmAI识别位点5r ^GTCTC(N)7 .3ri3 . .. C AG AG(N).…h▲BsmBI识别位点5. r,CGTCTC (N)J .3F3. ..GC AGAG (N)s^ ..5BsmFI识别位点覚…GGGAC(N)(D T . 3* 占…CCCTG(N),-A. . .5JBsmI识别位点5 . . . G A A T G C N* . . 3J3. . CT T AC^SN ..5JBsoBI识别位点5P. ..CVCG AG. .,3P3 . ^GRGCY^C . .5^Bsp1286l识别位点5 ….GDGC“b_・F 3〔…qHCGDG …5’BspCNI识别位点5\. CTCAG(N)/ . .3 3：…G AGTC (叽…BspDI识别位点5\ . A T T C G A T , , . 3"3：…T A G qj A「SBspEI识别位点5 …T T CCGGA. . ,3J3 . r AGGCCJ- . 5JBspHI识别位点5 ... T T C ATGA3’…A G T A CJ…翼BspMI识别位点5. ACC TGC (N)/. r. 33. .. TGG ACGBspQI识别位点5 . GCTCTTClNjJ .33LXGAGAAGtN)*^ r MiBsrBI识别位点5 . . . CCdtTC, . .3r3 GGC^pAG. . .5^BsrDI识别位点5；. . GC AATGNN*, .3r3 ：…C G T T A QN N …乳BsrFI识别位点5；, .R^ CGG Y . .. 3"3 ., YGGCC^R …5“BsrGI识别位点5 . lIST ACA .3…AC ATGJ…创BsrI识别位点5：.. ACTGGN T, r孑3：. . TG ACJDN .,. 5^BssHII识别位点5P.., G*CGCGC .. ,3P3.,.CGCGqp.,5^BssKI识别位点5 . . .'tCNGG . .3：.. GGNCq…hBssSI识别位点5 .(T A CGAG ,, .3"3F GTGCTJC .. .5^BstAPI识别位点5..GCANNNIS T NTGCCGT\NNNNACG8G5 阴BI识别位点5 . k. T T'b G A A t, 3J3. . AAGCJ T T5JBstEII识别位点5：.. G'fe T N A C C .. . 3^3 ... CCAhl TGJS.. .5^BstNI识别位点5：..cd\VGG. . . 3P3 . ..GGWCC . . 55：..CTCAG(N)tt^-专业资料分享BstUI识别位点5：.. CG'faG,. .3^3 . .. GCJ3C.. .5^BstXI识别位点5 ANNNNI S T N TGG.,3J3 . f.GGTN A NNNNNACC (5)BstYI识别位点5…R先ATdS3\, YCTAG^R.. .5JBstZ17l识别位点5…GT fT AC…卽3\. CA T^ATG.. .5-Bsu36l识别位点5.…CC?TN AGG. . . 3r3-…G G A N T& C. 5"Btgl识别位点5；,. C?bHYGG,. .33；, GG YRC^C , ,5JBtgZI识别位点5…GCGATG㈣「…3」3；..CGCTAC(N),4J...5,BtsCI识别位点5；. .GCAGTG N N T33：.. C G T C A (^N N ... 5'BtsI识别位点5.,,GC AGTG N N T.3P3；.. C G T C A <^N N ...Cac8l识别位点GCN T NGC . 3J3：…CGN/ICG…密ClaI识别位点ATfcGAT.. . 3J3 …T AGCJ A T. 5CspCI识别位点5 :的CAA (N>( GTGG (N)^.,33：・+严JN) GTT (M), CACC(N}^1V，,5J CviAII识别位点5…C T G…:T3 r.. G T A^C . . S PCviKI-1 识别位点5...RG T C Y (3)才…¥C#GR…5’CviQI识别位点S'-.J J TAC ,.3J3：5枣…5’Ddel识别位点5；-. C^TNAG.. .3J3；, G AN\C.Dpnl识别位点Ch,5 G A T C 3 3；. CT AG 5’DpnII识别位点5；r T GATC..33：r, CT AG^ . 5JDral识别位点5；.. T T T^AA …3’3\Rt A A A/TT . , 5JDralll 识别位点5；h CACNNN'bTG. . ,3“3…GT砂Nhl SC…5」Drdl识别位点5 . , GACNNNN T NWGTC, ,3 3：. . CTGNN^NNNNCAG., .5^Eael识别位点5“…gGCCR …;T3 . ..RCCGG/ .. 5JEael识别位点5, ^YfeGCCR, . 3J..RCCGG/ .Eagl识别位点5... C^SGCCG .专业资料分享3 .. GCCGGf. .Earl识别位点5 …CTCTTC (N)t\ .r3... GAGA AG (N)^. ..5PEcil识别位点5. . .GGCGGA(N)J . .3^GCCT (N)S A.., 5JEcoNI识别位点5： . . CCTNt/NNNAGG 33 . . GG ANNN^NNTCC . 5E CO O109I识别位点5P. . R(jfeNCC Y …:TS^.YCCNG/SR . . .5专业资料分享EcoP15l识别位点5 r C AGCAG(N)/ . P3r3 …GTCGTC(Wy 5EcoRI识别位点5 . ., A T T C ... 3 r 3；. . CTT AEcoRV识别位点5.GAT^TC -,3*3：.. CT AJ AG.. .5JFatl识别位点5：・先ATG…3」3\t GTAC^ .. 5’Faul识别位点5J CCCGC (N}J.. .3' 3；.. GGGCG (N)#A. . ,5J Fnu4HI识别位点昭…GC’NGC…朝3... CGN&G -SFokl识别位点5\L.GGATG(N)；1T3J3... CCTACtN),^ .5Fsel识别位点5 …GGCCGG'tc, .33 . CC/3GCCGG . . 5J Fspl识别位点5；,. TGC^bC A .. .3P3 ； ,. A C GJC G T (5)Haell识别位点5 RGCGC T Y. . .33 …LCGCGR …5，HaelllHgal识别位点5 . . .GACGC^N}/. 3... CTGCG<N}1(W. . 5" Hhal识别位点5 …GCdt …3" 宙…中CG…5，Hindi识别位点5：f GT Y T R AC.. .3r3\ .. CAR A YTG fHin dlll 识别位点5；. , A'A G C T T .…3" 3... TTCGA^A., .5JHi nfl识别位点5’ …GklMTC …3 3\., CT NAJG., .5* HinPII识别位点亦…dtGC…:r3 ... CGCp . . 5* Hpal识别位点5. .. GTT> AC.. .T3.., C A AJTG. _5J Hpall识别位点5“...cffeGG (3)3. GGC A C. 5JHphl识别位点51 …GGTGA (N)/.,.3, 3；.. CC AC T (N)7i,., 5" Hpy188l识别位点5 …T G “G A「3A0J CT …£Hpy188lll识别位点5.,TtfNNGA. ..3^3.,AGNN^CT. , ,5JHpy99l识别位点5；.. CGWCG T3：F i GCWGC.. ,5^HpyA V识别位点5.h CCTTC(N)J,3-3：. GGAAG(Nk ...5*%HpyCH4III识别位点h…A C N’G T …3"3 .,. T G^N C A … .5 HpyCH4IVHpyCH4Vm别位点5：. . TC?t A ,. .33■…A QG T…5」Kasl识别位点5.., G^J CGCC.,3P3r.^CCGCG^,..5J Kpnl识别位点5.,, GGT AC't... 3^3■…QCATGG …5，Mbol识专业资料分享别位点5P. .^3ATC .3^3P.. CT AG^. .5^Mboll识别位点专业资料分享5；., GA AGA (N「…3* 3；,, C T T C T (N)J A，. .5* Mfel识别位点5；.. C'X ATTG. ..3^3；T . GTT A Ajp . . 5JMlul识别位点5... /T CGCGT.,，3r3 ：.. T G C G C^A ... 5JMlyl识别位点5 …GAGTC(N, 一33\. .CTCA G (N) . 5-Mmel识别位点5； . .TCC RAC(N)M T.3' 3： . ,AG G YTG(NJ ia>. ..5*MnII识别位点5 ….CC「C (N}/ . 3 3…GGAG㈣立,5-Mscl识别位点5 , F TG(?fc：C A t,3P 3"…ACC^J GT.+J5JMsel识别位点5... f*T A A.. 3P3；T. A A TJ .. . 5^Msll识别位点5 …CAYNNV IN RYG. .,3J3 ...GTRNN^NNYAC (5)MspAII识别位点5P. . . CMCSfcKG, . .3 3•…GKYMC…扩Mspl识别位点5；F. dfcGG …3’3；. . GGqp …5’Mwol识别位点5 . . G C N N N N NVj N G C.. . 3\_ CGNN^NNN NNCG., Nael识别位点5 ... GCC\3GC,. .3r 3：…GGG&GG-.hNarl识别位点5 …GG^t GCC.. .3 3…CCGQGG …5“Ncil识别位点5 .C(7SGG,. ,3r 肚…GGS&C…・h Ncol识别位点5. ,,C T CATGG,. .3*3\ ,. G G T A CJ3 ... 5JNdel识别位点5； ., C /T T A T G ,. 3^ 3... GT A\AC…hNgoMlV识别位点5 . _ (itCGGC,. 3^ 3•…CGGCC^G. . 5Nhel识别位点5 . • T AGO . .,3"3 …GGATqp …hNlalll 识别位点亍…CATCf…3’AG…hNlalV识别位点5\,. GGt/NCC-.3 .. CCN^NGG.. .5JNmeAlll识别位点5：.. GCCGAG{N—G 3 ...CGGCTC(N)ia.^.^5rNotl识别位点5〔GdtGCCGC …;r3 ...CGCCGtyCG (5)Nrul识别位点5 . BT TCG^GA …3J3 …MCFCT …hNsil识别位点5 .ATGC^T T .. .3^ 3... I^ACGTA .. ,5JNspl识别位点5 . RCATG T Y. . 3J 3；.，X h GTACR.,.5rPacl识别位点5...TTAAf r TAA...3-3：+ , A ATJ A ATT T J+5JPaeR7l识别位点专业资料分享5；.. CG AG . ,3P. +G AGCTJC , . 5JPcil识别位点5：…A%島TGT….3*3* . . TGT A CA.. .S,APfIFl识别位点5；, .GAChiVlNGTC .…扩3 . . . C T G N N A N C A G ... 5* PflMI识别位点5； .. C C A N N N wTl T G G.. . 3^ 3；^GGTN A NNNNACC., .5^ Phol识别位点5：...GGfcC (3)3：. CCjjG.. 5JPlel识别位点5\.. GAGTC .3* 3：. CTCAG (N),,. hMiPmel识别位点5：. GTTT^AAC. . .3J3；.T C AAAJ T TG.T 5JPmll识别位点5；,, C AC^TG.. .33P.. GTGJ：AC- .5HPpuMI识别位点5 , h RdTj W c C Y ・”，3“3；_ YCC WGJ3R ., 5J PshAI识别位点5；ACNl7NNGTC . +.3J3 , . .CTGNNJNNC AG . .5'Psil识别位点5；., TT^T A A…X .. A A T A T T ... S HJLPspGl识别位点5 …1tC WGG …3“3；r,GG WCC^ ,.5PspOMI识别位点5；,. (fGGCCC.,. 33：…CCCGGJ3…5’PspXl识别位点5 V(7T CGAGB . b3J3. 8GAGC\CV.. .5*Pstl识别位点5 …CTGC 屆.33；. . G^ACGTC-. S-Pvul识别位点5 …CG ATEG …3" 3；.. GCJ AGC. . . 5JPvull识别位点5；…CAtSfcTG…富. . G T CJ3 A C ... 5^Rsal识别位点5...GT^C (3)3... CAJG.. .5*Rsrll识别位点5；, ,C(?fewCCG.. .3' 3：…GGC W枣C…hSacl识别位点5 GAGCT^ ,3J3；CJCGAG, . 5JSacll识别位点热…CCGC七G 23 .. GGjpGCC . 5JSall识别位点5 G T TCGAC,. 3J 3…CAGCT^S …hSapl识别位点5； .-GCTCTTCtN)^ ,3J 3 . r,CGAGAAG(N)(j.TJ5JSau3AI识别位点5 .. 7G A T c .,. 3^ 3：.. CT AG^..5JSau96l识别位点5... G^SNCC . . 33 …CCNGa ・h专业资料分享Sbfl识别位点TGCA T GG.,y3.., GG/CGTCC , 5’Seal识别位点5：.. A G T鼻C T ...甞3\,. TC AJGA . _5JScrFI识别位点5 ... CCT N GG.. 33\. GGNJSC , . 5JSexAl识别位点5 , .rfbCWGGT., ,3r3 …TGGWCqA. - SSfaNI识别位点5.., GC ATC (N)/ ,.. 3*3J.,. CGT AG (N)9A，.. 5*Sfcl识别位点5：…(?TR¥AG …;T3：. .GAYRT^C. . ,5Sfil识别位点专业资料分享5…GGCCNNNhTNGGCC .,3 3. ,CCGGN/tNNNCCGG .5'Sfol识别位点5；., GGC^CC ... 3J 3：…CCGfGG …5，SgrAI识别位点5 …C R’C CG G ¥ G …3"3. ,.G YGGCC jt AC,,,5HSmal识别位点5；., CCC^GG. . 3r3 …GEGfCC 一5"Smll识别位点S^.C T T Y R AG (3)3"…GARY .C …/SnaBI识别位点5 …T Ad A …3’3P r. A T G^： A T .. S HSpel识别位点5；. . zTCJ AGT . ,3r 3：…TGATC^A …hSphl识别位点5.GCATG'fe.. 3J3... CpT ACG . 5Sspl识别位点5 . A AT^TT 3P3. . . T T AJ AA . .5*Stul识别位点5. R1AGdtCT . ,.3f 3：…TCCJSGA. .5HStyD4l识别位点5 .. T CCNGG.,.『3’…GGNCq…5“Styl识别位点5仁C T CWWG G.. ,3r3；, GGWWC A C..Swal识别位点5 . , , A T T TV A A T ・・33 . h. T A A A J T T A ... 5^TaqaI识别位点5：.. T^G A . . ,33：…AGCJ ., 5JTlil识别位点5... <T T CGAG,,. 3J 3…GAGC"…5"Tsel识别位点5 …gfcwGC …3，3 …CGWQG …Tsp45l识别位点5；, ^STS AC.. 33\. .C ASTG^ . 5JTsp509l识别位点5….V A T T… 3-于…TT A. 5“TspMI识别位点C T CCGGG…3’3’…GGGCCf •…5’TspRI识别位点5 .NNCASTGNN T. ,3J3 .. ^NNGTSACNN., .5*Tth111l识别位点5P. . .GACN T NNGTC..3P3'…GTGN 叫NGAG-hXbal识别位点ftT AGA .3’…A G A T GJ…亍Xcml识别位点ANNNN N T J NNNTGG. 33. . .GGTNN N N^NNNNNACC. . 6Xhol识别位点占…C T TCGAG- 33 …GAGC T& …5"Xmal识别位点专业资料分享5：…CtCGGG…計3；.. GGGCqC . . .5* Xmnl识别位点5..,GA ANNVlN T TC . . 3r 3\ .. C T T N NJM N A A G .,. Zral识别位点5：. ,G AC T GTC■…孑3；, CTG A CAG...5-Tfil识别位点5 , . .G^WTC., .3 3 , . XT W .5。

A系列AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI 识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I 识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

常用NOT1和Sbf1识别位点(正向引物前加NOT1;反向引物前加sbf1.)AatII 识别位点GACGTCCTGCAGAcc65I 识别位点GTMKACCAKMTGAccI 识别位点GTMKACCAKMTGAciI 识别位点CCGCGGCGAclI 识别位点AACGTTTTGCAAAcuI 识别位点CTGAAGGACTTCAfeI 识别位点AGCGCTTCGCGAAflII 识别位点CTTAAGGAATTCAflIII 识别位点ACRYGTTGYRCAAgeI 识别位点ACCGGTTGGCCAAhdI 识别位点GACNNNNNGTCCTGNNNNNCAGAleI 识别位点CACNNNNGTGGTGNNNNCACAluI 识别位点AGCTTCGAAlwI 识别位点GGATCCCTAGAlwNI 识别位点CAGNNNCTGGTCNNNGACApaI 识别位点GGGCCCCCCGGGGTGCACCACGTGApeKI 识别位点GCWGCCGWCGApoI 识别位点RAATTYYTTAARAscI 识别位点GGCGCGCCCCGCGCGGAseI 识别位点ATTAATTAATTAAsiSI 识别位点GCGATCGCCGCTAGCGAvaI 识别位点CYCGRGGRGCYCAvaII 识别位点GGWCCCCWGGCCTAGGGGATCCBaeI 识别位点NACNGTAYCNBamHI 识别位点GGATCCCCTAGGBanI 识别位点GGYRCCCCRYGGBanII 识别位点GRGCYCCYCGRGBbsI 识别位点GAAGACCTTCTGBbvCI 识别位点CCTCAGCGGAGTCGBbvI 识别位点GCAGCCGTCGCCATCGGTAGBceAI 识别位点ACGGC TGCCGBcgI 识别位点CGANTGNGCTNACGBciVI 识别位点GTATCCCATAGGBclI 识别位点TGATCAACTACTBfaI 识别位点CTAGGATCBfuAI 识别位点ACCTGCTGGACGBglI 识别位点GCCNNNNNGGCCGGNNNNNCCGAGATCTTCTAGABlpI 识别位点GCTNAGCCGANTCGBme1580I 识别位点GKGCMCCMCGKGBmgBI 识别位点CACGTCGTGCAGBmrI 识别位点ACTGGGNTGACCCNBmtI 识别位点GCTAGCCGATCGBpmI 识别位点CTGGAGNGACCTCNBpu10I 识别位点CCTNAGCGGANTCGCTTGAGNGAACTCNBsaAI 识别位点YACGTRRTGCAYBsaBI 识别位点GATNNNNATCCTANNNNTAGBsaHI 识别位点GRCGYCCYGCRGBsaI 识别位点GGTCTCNCCAGAGNBsaJI 识别位点CCNNGGGGNNCCBsaWI 识别位点WCCGGWWGGCCWBsaXI 识别位点NACNCTCCNNTGNGAGGNGAGGAGNCTCCTCNBseYI 识别位点CCCAGCGGGTCGBsgI 识别位点GTGCAGCACGTCBsiEI 识别位点CGRYCGGCYRGCBsiHKAI 识别位点GWGCWCCWCGWGBsiWI 识别位点CGTACGGCATGCBslI 识别位点CCNNNNNNNGGGGNNNNNNNCCBsmAI 识别位点GTCTCNCAGAGNCGTCTCNGCAGAGNBsmFI 识别位点GGGACCCCTGBsmI 识别位点GAATGCNCTTACGNBsoBI 识别位点CYCGRGGRGCYCBsp1286I 识别位点GDGCHCCHCGDGBspCNI 识别位点CTCAGN GAGTCNBspDI 识别位点ATCGATTAGCTABspEI 识别位点TCCGGAAGGCCTTCATGAAGTACTBspMI 识别位点ACCTGCNTGGACGNBspQI 识别位点GCTCTTCNCGAGAAGNBsrBI 识别位点CCGCTCGGCGAGBsrDI 识别位点GCAATGNNCGTTACNNBsrFI 识别位点RCCGGYYGGCCRBsrGI 识别位点TGTACAACATGTBsrI 识别位点ACTGGNTGACCNGCGCGCCGCGCGBssKI 识别位点CCNGGGGNCCBssSI 识别位点CACGAGGTGCTCBstAPI 识别位点GCANNNNNTGCCGTNNNNNACGBstBI 识别位点TTCGAAAAGCTTBstEII 识别位点GGTNACCCCANTGGBstNI 识别位点CCWGGGGWCCBstUI 识别位点CGCGGCGCCCANNNNNNTGGGGTNNNNNNACCBstYI 识别位点RGATCYYCTAGRBstZ17I 识别位点GTATACCATATGBsu36I 识别位点CCTNAGGGGANTCCBtgI 识别位点CCRYGGGGYRCCBtgZI 识别位点GCGATGCGCTACBtsCI 识别位点GCAGTGNNCGTCACNNBtsI 识别位点GCAGTGNNCGTCACNNGCNNGCCGNNCGClaI 识别位点ATCGATTAGCTACspCI 识别位点NCAANGTGGNNGTTNCACCNCviAII 识别位点CATGGTACCviKI-1识别位点RGCYYCGRCviQI 识别位点GTACCATGDdeI 识别位点CTNAGGANTCDpnI 识别位点GATCCTAGGATCCTAGDraI 识别位点TTTAAAAAATTTDraIII 识别位点CACNNNGTGGTGNNNCACDrdI 识别位点GACNNNNNNGTCCTGNNNNNNCAGEaeI 识别位点YGGCCRRCCGGYEagI 识别位点CGGCCGGCCGGCEarI 识别位点CTCTTCGAGAAGEciI 识别位点GGCGGACCGCCTCCTNNNNNAGGGGANNNNNTCCEcoO109I 识别位点RGGNCCYYCCNGGREcoP15I 识别位点CAGCAGGTCGTCEcoRI 识别位点GAATTCCTTAAGEcoRV 识别位点GATATCCTATAGFatI 识别位点CATGGTACFauI 识别位点CCCGCGGGCGFnu4HI 识别位点GCNGCCGBCGGGATGNCCTACNFseI 识别位点GGCCGGCCCCGGCCGGFspI 识别位点TGCGCAACGCGTHaeII 识别位点RGCGCYYCGCGRHaeIIIHgaI 识别位点GACGCCTGCGHhaI 识别位点GCGCCGCGHincII 识别位点GTYRACCARYTGHindIII 识别位点AAGCTTTTCGAAGANTCCTNAGHinP1I 识别位点GCGCCGCGHpaI 识别位点GTTAACCAATTGHpaII 识别位点CCGGGGCCHphI 识别位点GGTGACCACTHpy188I 识别位点TCNGAAGNCTHpy188III 识别位点TCNNGAAGNNCTHpy99I 识别位点CGWCGGCWGCCCTTCGGAAGHpyCH4III 识别位点ACNGTTGCCAHpyCH4IVHpyCH4V 识别位点TGCAACGTKasI 识别位点GGCGCCCCGCGGKpnI 识别位点GGTACCCCATGGMboI 识别位点GATCCTAGMboII 识别位点GAAGACTTCTMfeI 识别位点CAATTGGTTAACACGCGTTGCGCAMlyI 识别位点GAGTCCTCAGMmeI 识别位点TCCRACAGGYTGMnlI 识别位点CCTCGGAGMscI 识别位点TGGCCAACCGGTMseI 识别位点TTAAAATTMslI 识别位点CAYNNNNRTGGTRNNNNYACMspA1I 识别位点CMGCKGGKCGMCCCGGGGCCMwoI 识别位点GCNNNNNNNGCCGNNNNNNNCGNaeI 识别位点GCCGCCGGCCGNarI 识别位点GGCGCCCCGCGGNciI 识别位点CCSGGGGSCCNcoI 识别位点CCATGGGGTACCNdeI 识别位点CATATG GTATACNgoMIV识别位点GCCGGC CGGCCGGCTAGCCGATCGNlaIII 识别位点CATGGTACNlaIV 识别位点GGNNCCCCNNGGNmeAIII 识别位点GCCGAGCGGCTCNotI 识别位点GCGGCCGC CGCCGGCGNruI 识别位点TCGCGAAGCGCTNsiI 识别位点ATGCAT TACGTANspI识别位点RCATGY YGTACRTTAATTAA AATTAATTPaeR7I识别位点CTCGAG GAGCTCPciI 识别位点ACATGT TGTACAPflFI识别位点GACNNNGTC CTGNNNCAGPflMI 识别位点CCANNNNNTGGGGTNNNNNACCPhoI 识别位点GGCCPleI识别位点GAGTCN CTCAGNPmeI 识别位点GTTTAAAC CAAATTTGPmlI识别位点CACGTG GTGCACRGGWCCYYCCWGGRPshAI 识别位点GACNNNNGTCCTGNNNNCAGPsiI 识别位点TTATAAAATATTPspGI 识别位点CCWGGGGWCCPspOMI 识别位点GGGCCCCCCGGGPspXI 识别位点VCTCGAGBBGAGCTCVPstI 识别位点CTGCAGGACGTCPvuI 识别位点CGATCGGCTAGCCAGCTGGTCGACRsaI 识别位点GTACCATGRsrII 识别位点CGGWCCGGCCWGGCSacI 识别位点GAGCTCCTCGAGSacII 识别位点CCGCGGGGCGCCSalI 识别位点GTCGACCAGCTGSapI 识别位点GCTCTTCNCGAGAAGNSau3AI 识别位点GATCCTAGGGNCCCCNGGSbfI 识别位点CCTGCAGGGGACGTCCScaI 识别位点AGTACTTCATGAScrFI 识别位点CCNGGGGNCCSexAI 识别位点ACCWGGTTGGWCCASfaNI 识别位点GCATCNCGTAGNSfcI 识别位点CTRYAGGAYRTCSfiI 识别位点GGCCNNNNNGGCCCCGGNNNNNCCGGGGCGCCCCGCGGSgrAI 识别位点CRCCGGYGGYGGCCRCSmaI 识别位点CCCGGGGGGCCCSmlI 识别位点CTYRAGGARYTCSnaBI 识别位点TACGTAATGCATSpeI 识别位点ACTAGTTGATCASphI 识别位点GCATGCCGTACGSspI 识别位点AATATTTTATAAStuI 识别位点AGGCCTTCCGGAStyD4I 识别位点CCNGGGGNCCStyI 识别位点GCWWGGGGWWCCSwaI 识别位点ATTTAAATTAAATTTATaqαI 识别位点TCGAAGCTTfiI 识别位点GAWTCCTWAGTliI 识别位点CTCGAGGAGCTCTseI 识别位点GCWGCCGWCGTsp45I 识别位点GTSACCASTGTsp509I 识别位点AATTTTAATspMI 识别位点CCCGGGGGGCCCTspRI 识别位点NNCASTGNNNNGTSACNNTth111I 识别位点GACNNNGTCCTGNNNCAGXbaI 识别位点TCTAGAAGATCTXcmI 识别位点CCANNNNNNNNNTGGGGTNNNNNNNNNACCXhoI 识别位点CTCGAGGAGCTCXmaI识别位点CCCGGG GGGCCCXmnI识别位点GAANNNNTTC CTTNNNNAAGZraI识别位点GACGTC CTGCAG与大家共享（非原创）。

Aatn识别位点Aval识别位点Acc65I识别位点Avail识别位点AccI识别位点Avril iR别位点Ac订识别位点Bael识别位点BamHI识别位点Acll识别位点BanI识别位点Acul识别位点Banll识别位点Afel识别位点BbsI识别位点AfllliR别位点BbvCI识别位点Afllll识别位点Bbvl识别位点Agel识别位点Bed识别位点AhdI识别位点BceAI识别位点Alel识别位点Bcgl识别位点Alul识别位点BciVI识别位点Alwl识别位点Bell识别位点AlwNI识别位点Bfal识别位点Apal识别位点BfuAI谋别位点ApaLI识别位点Bgll识别位点ApeKI駅别位点Bglll识别位点Apol识别位点BlpI识别位点Asci识别位点Bmel580I识别位点Asel识别位点BmgBI识别位点AsiSI识别位点Bmrl识别位点Bmtl识别位点BsoBI识别位点Bpml识别位点BSP1286I识别位点BpulOI识别位点BspCNI识别位点BpuEI识别位点5'…CTC AG(N),・・・3'3：・.GAGTC(Nh「.5' BspDI课别位点BsaAI駅别位点BspEI识别位点BsaBI识别位点BspHI识别位点BsaHI识别位点BspMI谋别位点Bsal识别位点BspQI识别位点BsaJI识别位点BsrBI识别位点BsaffI駅别位点BsrDI识别位点BsaXI识别位点BsrFI识别位点BseRI识别位点BsrGI识别位点BseYI识别位点BsrI识别位点Bsgl识则位点BssHII识别位点BsiEI駅别位点BssKI识别位点BsiHKAI识别位点BssSI识别位点BsiWI识别位点BstAPI识别位点BsH识别位点BstBI识别位点BsmAI识别位点BstEII识别位点BsmBI识别位点BstNI识别位点BsmFI识别位点BstUI识别位点Bsml识别位点5-..CTCAGCN)/-.3^ 釦° 3〔・・GAGTC(NL .…5・BstXI识别位点EagI识别位点BstYI识别位点Earl识别位点BstZ17I识别位点Ecil识别位点BSU36I识别位点EcoNI帜别位点ECO0109I识别位点Btgl识别位点EcoPlSI识别位点BtgZI识别位点EcoRI识别位点BtsCI识别位点EcoRV识别位点BtsI识别位点Fatl识别位点Cac8I识别位点Faul识别位点Clal识别位点Fnu4HI识别位点CspCI识别位点FokI识别位点CviAII识别位点Fsel识别位点CviKI-1识别位点FspI识别位点CviQI识别位点Haell识别位点Ddel识别位点Dpnl识别位点Haelll Hgal识别位点Dpnll识别位点Hhal识别位点Dral识别位点Hindi识别位点Drain识别位点Hindlll识别位点DrdI识别位点Hinfl识别位点Eael识别位点HinPH识别位点Eael识别位点Hpal识别位点Hpall识别位点Mwol识别位点HphI识别位点Nael识别位点Hpyl88I识别位点Narl识别位点Hpyl88III识别位点Nc订识别位点Hpy99I识别位点Ncol识别位点HpyAV识别位点Ndel识别位点HpyCH4nI识则位点NgoMIV识别位点HpyCH4IVHpyCH4V识别位点Nhel识别位点KasI识别位点Nlalll识别位点Kpnl识别位点NlalV识别位点Mbol识别位点NmeAIII识别位点MboII识别位点Notl识别位点Mfel识别位点Nrul识别位点M1U1识则位点Nsil识别位点Mlyl识别位点NspI识别位点Mmel识别位点Pad识别位点Mnll识别位点PaeR7I识别位点MscI识别位点Pc订识别位点Msel识别位点Pf 1FI识别位点MslIiR别位点PflMI识别位点MspAlI识别位点Phol识别位点MspI识别位点Piel识别位点Pmel识别位点SexAI识别位点Pmll识别位点SfaNI课别位点PpuMI识别位点Sfcl识别位点PshAI识别位点Sf订识别位点Ps订识别位点Sfol识别位点PspGI识别位点SgrAI说别位点PspOMI识别位点Smal识别位点PspXI识别位点Smll识别位点PstI识别位点SnaBI识别位点Pvul识别位点Spel识别位点PvuII识别位点SphI说别位点Rsal识别位点SspI识别位点RsrII识别位点Stul识别位点Sad识则位点StyD4I识别位点SacII駅别位点Styl识别位点Sall识别位点Swal识别位点SapI识别位点TaqaI识别位点Sau3AI识别位点Tf订识别位点Sau96I识别位点T1订识别位点Sbfl识别位点Tsel识别位点Seal识别位点Tsp45I识别位点ScrFI识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点TthlllI识别位点Xbal识别位点Xcml识别位点Xhol识别位点Xmal识别位点XmnI识别位点Zral识别位点与大家共專（非原创）。

AatII识别位点GACGTCCTGCAGAcc65I识别位点GTMKAC CAKMTGAccI识别位点GTMKAC CAKMTGAciI识别位点CCGCGGCGAclI识别位点AACGTT TTGCAAAcuI识别位点CTGAAG GACTTCAfeI识别位点AGCGCT TCGCGAAflII识别位点CTTAAG GAATTCAflIII识别位点ACRYGT TGYRCA AgeI识别位点ACCGGT TGGCCAAhdI识别位点GACNNNNNGTC CTGNNNNNCAGAleI识别位点CACNNNNGTG GTGNNNNCACAluI识别位点AGCTTCGAAlwI识别位点GGATC CCTAGAlwNI识别位点CAGNNNCTG GTCNNNGACApaI识别位点GGGCCC CCCGGGApaLI识别位点GTGCAC CACGTGApeKI识别位点GCWGCCGWCGApoI识别位点RAATTY YTTAARAscI识别位点GGCGCGCC CCGCGCGGAseI识别位点ATTAAT TAATTAAsiSI识别位点GCGATCGC CGCTAGCGAvaI识别位点CYCGRG GRGCYCAvaII识别位点GGWCC CCWGGAvrII识别位点CCTAGG GGATCCBaeI识别位点NACNGTAYCN BamHI识别位点GGATCC CCTAGGBanI识别位点GGYRCC CCRYGGBanII识别位点GRGCYC CYCGRGBbsI识别位点GAAGAC CTTCTGBbvCI识别位点CCTCAGC GGAGTCGBbvI识别位点GCAGC CGTCGBccI识别位点CCATC GGTAGBceAI识别位点ACGGC TGCCGBcgI识别位点CGANTGN GCTNACGBciVI识别位点GTATCCCATAGGBclI识别位点TGATCAACTACTBfaI识别位点CTAGGATCBfuAI识别位点ACCTGCTGGACGBglI识别位点GCCNNNNNGGC CGGNNNNNCCGBglII识别位点AGATCTTCTAGABlpI识别位点GCTNAGC CGANTCGBme1580I识别位点GKGCMCCMCGKG CACGTC GTGCAGBmrI识别位点ACTGGGN TGACCCNBmtI识别位点GCTAGC CGATCGBpmI识别位点CTGGAGN GACCTCNBpu10I识别位点CCTNAGC GGANTCGBpuEI识别位点CTTGAGN GAACTCNBsaAI识别位点YACGTR RTGCAYBsaBI识别位点GATNNNNATC CTANNNNTAGBsaHI识别位点GRCGYC CYGCRGBsaI识别位点GGTCTCN CCAGAGNBsaJI识别位点CCNNGG GGNNCCBsaWI识别位点WCCGGW WGGCCWBsaXI识别位点NACNCTCCN NTGNGAGGNBseRI识别位点GAGGAGN CTCCTCNBseYI识别位点CCCAGC GGGTCGBsgI识别位点GTGCAG CACGTCBsiEI识别位点CGRYCG GCYRGC GWGCWC CWCGWGBsiWI识别位点CGTACGGCATGCBslI识别位点CCNNNNNNNGG GGNNNNNNNCCBsmAI识别位点GTCTCNCAGAGNBsmBI识别位点CGTCTCN GCAGAGNBsmFI识别位点GGGACCCCTGBsmI识别位点GAATGCN CTTACGNBsoBI识别位点CYCGRGGRGCYCBsp1286I识别位点GDGCHC CHCGDGBspCNI识别位点CTCAGN GAGTCNBspDI识别位点ATCGAT TAGCTABspEI识别位点TCCGGA AGGCCTBspHI识别位点TCATGA AGTACTBspMI识别位点ACCTGCN TGGACGNBspQI识别位点GCTCTTCN CGAGAAGNBsrBI识别位点CCGCTC GGCGAGBsrDI识别位点GCAATGNN CGTTACNN RCCGGY YGGCCRBsrGI识别位点TGTACA ACATGTBsrI识别位点ACTGGN TGACCNBssHII识别位点GCGCGC CGCGCGBssKI识别位点CCNGG GGNCCBssSI识别位点CACGAG GTGCTCBstAPI识别位点GCANNNNNTGC CGTNNNNNACGBstBI识别位点TTCGAA AAGCTTBstEII识别位点GGTNACC CCANTGGBstNI识别位点CCWGG GGWCCBstUI识别位点CGCGGCGCBstXI识别位点CCANNNNNNTGG GGTNNNNNNACCBstYI识别位点RGATCY YCTAGRBstZ17I识别位点GTATAC CATATGBsu36I识别位点CCTNAGG GGANTCCBtgI识别位点CCRYGG GGYRCCBtgZI识别位点GCGATG CGCTAC GCAGTGNN CGTCACNNBtsI识别位点GCAGTGNN CGTCACNNCac8I识别位点GCNNGC CGNNCGClaI识别位点ATCGAT TAGCTACspCI识别位点NCAANGTGGN NGTTNCACCNCviAII识别位点CATGGTACCviKI-1识别位点RGCYYCGRCviQI识别位点GTACCATGDdeI识别位点CTNAGGANTCDpnI识别位点GATCCTAGDpnII识别位点GATCCTAGDraI识别位点TTTAAA AAATTTDraIII识别位点CACNNNGTG GTGNNNCACDrdI识别位点GACNNNNNNGTC CTGNNNNNNCAGEaeI识别位点YGGCCR RCCGGYEagI识别位点CGGCCG GCCGGCEarI识别位点CTCTTC GAGAAG EciI识别位点GGCGGACCGCCTEcoNI识别位点CCTNNNNNAGG GGANNNNNTCCEcoO109I识别位点RGGNCCY YCCNGGREcoP15I识别位点CAGCAGGTCGTCEcoRI识别位点GAATTCCTTAAGEcoRV识别位点GATATCCTATAGFatI识别位点CATGGTACFauI识别位点CCCGCGGGCGFnu4HI识别位点GCNGCCGBCGFokI识别位点GGATGN CCTACNFseI识别位点GGCCGGCC CCGGCCGGFspI识别位点TGCGCA ACGCGTHaeII识别位点RGCGCY YCGCGRHaeIIIHgaI识别位点GACGCCTGCGHhaI识别位点GCGCCGCGHincII识别位点GTYRAC CARYTG HindIII识别位点AAGCTTTTCGAAHinfI识别位点GANTCCTNAGHinP1I识别位点GCGCCGCGHpaI识别位点GTTAACCAATTGHpaII识别位点CCGGGGCCHphI识别位点GGTGACCACTHpy188I识别位点TCNGAAGNCTHpy188III识别位点TCNNGAAGNNCTHpy99I识别位点CGWCGGCWGCHpyAV识别位点CCTTCGGAAGHpyCH4III识别位点ACNGTTGCCAHpyCH4IV HpyCH4V识别位点TGCAACGTKasI识别位点GGCGCCCCGCGGKpnI识别位点GGTACCCCATGGMboI识别位点GATCCTAGMboII识别位点GAAGACTTCTMfeI识别位点CAATTGGTTAAC ACGCGT TGCGCAMlyI识别位点GAGTCCTCAGMmeI识别位点TCCRAC AGGYTGMnlI识别位点CCTCGGAGMscI识别位点TGGCCA ACCGGTMseI识别位点TTAAAATTMslI识别位点CAYNNNNRTG GTRNNNNYACMspA1I识别位点CMGCKG GKCGMCMspI识别位点CCGGGGCCMwoI识别位点GCNNNNNNNGC CGNNNNNNNCGNaeI识别位点GCCGC CGGCCGNarI识别位点GGCGCC CCGCGGNciI识别位点CCSGGGGSCCNcoI识别位点CCATGG GGTACCNdeI识别位点CATATG GTATACNgoMIV识别位点GCCGGC CGGCCGNheI识别位点GCTAGC CGATCG CATGGTACNlaIV识别位点GGNNCC CCNNGGNmeAIII识别位点GCCGAG CGGCTCNotI识别位点GCGGCCGC CGCCGGCGNruI识别位点TCGCGA AGCGCTNsiI识别位点ATGCATTACGTANspI识别位点RCATGYYGTACRPacI识别位点TTAATTAA AATTAATTPaeR7I识别位点CTCGAG GAGCTCPciI识别位点ACATGT TGTACAPflFI识别位点GACNNNGTC CTGNNNCAGPflMI识别位点CCANNNNNTGG GGTNNNNNACCPhoI识别位点GGCCPleI识别位点GAGTCN CTCAGNPmeI识别位点GTTTAAAC CAAATTTGPmlI识别位点CACGTG GTGCACPpuMI识别位点RGGWCCY YCCWGGR GACNNNNGTC CTGNNNNCAGPsiI识别位点TTATAAAATATTPspGI识别位点CCWGG GGWCCPspOMI识别位点GGGCCC CCCGGGPspXI识别位点VCTCGAGB BGAGCTCVPstI识别位点CTGCAG GACGTCPvuI识别位点CGATCG GCTAGCPvuII识别位点CAGCTG GTCGACRsaI识别位点GTACCATGRsrII识别位点CGGWCCG GCCWGGCSacI识别位点GAGCTC CTCGAGSacII识别位点CCGCGG GGCGCCSalI识别位点GTCGAC CAGCTGSapI识别位点GCTCTTCN CGAGAAGNSau3AI识别位点GATCCTAGSau96I识别位点GGNCC CCNGGSbfI识别位点CCTGCAGG GGACGTCC AGTACTTCATGAScrFI识别位点CCNGGGGNCCSexAI识别位点ACCWGGT TGGWCCASfaNI识别位点GCATCN CGTAGNSfcI识别位点CTRYAG GAYRTCSfiI识别位点GGCCNNNNNGGCC CCGGNNNNNCCGGSfoI识别位点GGCGCC CCGCGGSgrAI识别位点CRCCGGYG GYGGCCRCSmaI识别位点CCCGGG GGGCCCSmlI识别位点CTYRAG GARYTCSnaBI识别位点TACGTA ATGCATSpeI识别位点ACTAGT TGATCASphI识别位点GCATGC CGTACGSspI识别位点AATATT TTATAAStuI识别位点AGGCCT TCCGGAStyD4I识别位点CCNGG GGNCCStyI识别位点GCWWGG GGWWCCSwaI识别位点ATTTAAAT TAAATTTATaqαI识别位点TCGAAGCTTfiI识别位点GAWTCCTWAGTliI识别位点CTCGAG GAGCTCTseI识别位点GCWGCCGWCGTsp45I识别位点GTSACCASTGTsp509I识别位点AATTTTAATspMI识别位点CCCGGG GGGCCCTspRI识别位点NNCASTGNN NNGTSACNNTth111I识别位点GACNNNGTC CTGNNNCAGXbaI识别位点TCTAGAAGATCTXcmI识别位点CCANNNNNNNNNTGG GGTNNNNNNNNNACCXhoI识别位点CTCGAGGAGCTCXmaI识别位点CCCGGGGGGCCCXmnI识别位点GAANNNNTTC CTTNNNNAAGZraI识别位点GACGTCCTGCAG与大家共享〔非原创〕。