Identification and characterization of cDNA sequences encoding the HIS3 and LEU2 genes of the f
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医学微生物学英语Microbiology is a fascinating field that delves into the intricate world of microscopic organisms, playing a pivotal role in the realm of medicine. From the study of bacteria, viruses, and other microbes, to the understanding of their impact on human health, microbiology has been at the forefront of scientific advancements.One of the primary focuses of medical microbiology is the identification and characterization of pathogenic microorganisms. These are the microbes that can cause various diseases and infections in the human body. By understanding the unique features and behaviors of these microbes, medical professionals can develop effective strategies for diagnosis, treatment, and prevention of infectious diseases.The field of medical microbiology encompasses a wide range of specialized areas. Bacteriology, for instance, involves the study of bacteria, their structure, metabolism, and the ways in which they can either benefit or harm human health. Virology, on the other hand, focuses on the study of viruses, their genetic composition, and their ability to infect and replicate within host cells.Another important aspect of medical microbiology is the study of the human microbiome. The human body is home to a vast and diverse community of microorganisms, collectively known as the microbiome. These microbes play a crucial role in maintaining a healthy immune system, aiding in the digestion of food, and even influencing the development of the brain and nervous system.Understanding the delicate balance of the microbiome and how it can be disrupted by factors such as diet, antibiotic use, and environmental exposures is a critical area of research in medical microbiology. Imbalances in the microbiome have been linked to a variety of health conditions, including inflammatory bowel diseases, obesity, and even certain mental health disorders.In the realm of diagnosis and treatment, medical microbiologists play a vital role. They develop and refine techniques for the rapid and accurate identification of infectious agents, allowing healthcare providers to make informed decisions about the most appropriate course of action. This can include the use of advanced laboratory techniques, such as polymerase chain reaction (PCR) and next-generation sequencing, to detect the presence of specific microbes.Moreover, medical microbiologists contribute to the development of new antimicrobial agents, such as antibiotics and antiviral drugs, to combat the growing threat of drug-resistant microbes. As pathogensevolve and become more resilient, the need for innovative and effective therapies becomes increasingly urgent.Beyond the clinical setting, medical microbiologists also play a crucial role in public health and epidemiology. They investigate outbreaks of infectious diseases, tracing the sources and transmission patterns of microbes, and implementing strategies to control and prevent the spread of these diseases within communities.The field of medical microbiology is constantly evolving, with new discoveries and advancements being made every day. From the development of cutting-edge diagnostic tools to the exploration of the human microbiome, the contributions of medical microbiologists have a profound impact on the health and well-being of individuals and populations worldwide.As we continue to navigate the complex and ever-changing landscape of infectious diseases, the expertise and dedication of medical microbiologists will remain essential in our efforts to maintain and improve global health.。
国际种子检验规程 2023版1.国际种子检验规程的目的是促进国际贸易,并保障种子的质量和安全。
The purpose of the International Seed Testing Regulations is to facilitate international trade and ensure the quality and safety of seeds.2.种子检验应遵循规程列出的标准和程序。
Seed testing should follow the standards and procedures outlined in the regulations.3.每个国家应该建立或指定一个种子检验机构,并确保其符合规程的要求。
Each country should establish or designate a seed testing organization and ensure that it complies with the requirements of the regulations.4.种子检验应在指定的实验室条件下进行,以确保结果的准确性和可靠性。
Seed testing should be conducted under specifiedlaboratory conditions to ensure the accuracy and reliabilityof the results.5.种子样品的采集、保存和运输应符合规程的要求,以防止污染和损坏。
The collection, preservation, and transportation of seed samples should comply with the requirements of theregulations to prevent contamination and damage.6.种子检验应包括对种子外观、纯度、发芽率和种子病害的检测。
CHAPTER 8Identification and Characterization of Streptococcus pneumoniae肺炎链球菌是一种可以在细胞内或细胞外寄生革兰氏阳性球菌,以单个或短链的形式存在。
该菌的生长条件较为严格,最适宜生长条件为35-37℃、5%CO2。
通常可以在血平板(BAP)或巧克力平板(CAP)上生长。
在血平板上菌落形态较小、灰色、湿润有时候有粘液,有α溶血现象(图1)。
这种溶血特性可以将该菌与很多细菌区分开,但是无法与草绿色链球菌区别。
但是该菌在培养24-28小时后,菌落形态表现为平坦、中心凹陷,而草绿色链球球菌不会出现以上变化(图2)。
利用显微镜对菌落形态的观察有利于鉴别两种菌。
在鉴定之前一定要分纯培养。
在下列的鉴定程序中,应着重鉴别有肺炎链球菌的α溶血特性,35-37℃、5%CO2条件下培养时间<24小时经典肺炎链球菌菌落均会出现这种溶血特性。
下面的试验用于在BAP平板上鉴定肺炎双球菌相及相似菌落(图3)。
肺炎链球菌可以通过同时进行革兰氏染色,触酶试验,奥普托欣敏感性试验进行鉴定,用胆盐溶解试验进行确证试验。
如果这些试验鉴定是肺炎链球菌时,则进行血清分型。
辅助用于鉴定肺炎链球菌分子分型方法将在第10章:PCR技术和第12章:分子技术中分别进行介绍。
其他的方法见网址/ncidod/biotech/strep/strep-doc/index.htm.由于此种细菌对于相关操作人员及环境存在潜在的危险,生物安全2级试验室是进行肺炎链球菌相关操作的必要条件。
根据第4章的具体要求要求进行防护和操作。
图1.在血琼脂平板上,肺炎链球菌周围出现草绿色的α溶血环(黑色箭头所指)图2.在血琼脂平板上,肺炎链球菌生长24-28小时后,菌落扁平、中央凹陷;而草绿色链球菌菌落中央仍然保持凸起翻译人:limachao@Figure 3.肺炎链球菌鉴别流程图I.触酶试验触酶能够将H2O2分解为H2O和O2。
专利名称:Identification and characterization of a novel alpha-amylase from maize endosperm发明人:Martha G. James,Alan M. Myers,Christophe Colleoni,Kevin D. Stokes申请号:US10952551申请日:20040927公开号:US07270988B2公开日:20070918专利内容由知识产权出版社提供摘要:SHE, a Starch Hydrolytic Enzyme active in maize endosperm (), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, , apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.申请人:Martha G. James,Alan M. Myers,Christophe Colleoni,Kevin D. Stokes地址:Des Moines IA US,Ames IA US,Arques FR,Ames IA US国籍:US,US,FR,US代理机构:Weingarten, Schurgin, Gagnebin & Lebovici LLP 更多信息请下载全文后查看。
超声英文文献分享以下是一篇关于超声的英文文献分享:Title: The Use of Ultrasound in the Management of Thyroid NodulesUltrasound (US) is a widely used imaging modality that provides valuable information in the evaluation and management of thyroid nodules. US allows for the identification and characterization of thyroid nodules, evaluation of nodule vascularity, and guidance for fine-needle aspiration (FNA). In this article, we discuss the role of US in the diagnosis and management of thyroid nodules, including its advantages, limitations, and future directions.US is a noninvasive, radiation-free, and cost-effective imaging modality that provides real-time information about thyroid nodules. It can identify small nodules that are often missed on palpation and assess the morphology, size, echogenicity, calcifications, and vascularity of thyroid nodules. US-guided FNA is a minimally invasive technique that allows for the histopathological diagnosis of thyroidnodules. It has replaced surgical biopsy as the reference standard for the diagnosis of thyroid nodules.US can be used to differentiate benign from malignant thyroid nodules with variable accuracy. Malignant thyroid nodules are often hypoechoic, have irregular borders, microcalcifications, and increased vascularity on Doppler US. However, there is significant overlap between benign and malignant thyroid nodules on US features, leading to false positives and false negatives. Therefore, US cannot be used alone to diagnose thyroid malignancy.US-guided FNA is indicated for the evaluation of thyroid nodules with suspicious US features or a diameter greater than 1 cm. The Bethesda System for Reporting Thyroid Cytopathology is a widely used classification system for reporting FNA results. It categorizes thyroid nodules as benign, atypia of undetermined significance or follicular lesion of undetermined significance (AUS/FLUS), follicular neoplasm or suspicious for follicular neoplasm (FN/SFN), suspicious for malignancy (SM), or malignant. The risk of malignancy varies with the FNA category and informs clinical management decisions.Management options for thyroid nodules include observation, US-guided FNA, or surgical excision. Management decisions should be individualized based on the risk of malignancy, patient preferences, and local resources. Observation is recommended for benign thyroid nodules with low risk of malignancy. US-guided FNA is indicated for thyroid nodules with suspicious US features or a diameter greater than 1 cm. Surgical excision is indicated for cytologically malignant thyroid nodules or those with high suspicion of malignancy based on US features or growth on serial US surveillance.In conclusion, US plays a crucial role in the evaluation and management of thyroid nodules. It provides valuable information about the morphology and vascularity of thyroid nodules and can guide FNA for histopathological diagnosis. However, US features overlap between benign and malignant thyroid nodules, limiting its diagnostic accuracy. Therefore, US should be used in conjunction with other clinical factors and cytological findings to optimize the diagnosis and management of thyroid nodules. Future research should focus on improving US technology and developing more accurate algorithms for thyroid nodule diagnosis.。
红外光谱特征峰对照表英文Infrared Spectroscopy Characteristic Peak Reference Table.Infrared spectroscopy is a valuable tool for the identification and characterization of compounds, as it provides information about the vibrations of the chemical bonds within a molecule. The infrared spectrum is typically divided into several regions based on the frequency of the vibrations, and each region corresponds to different typesof chemical bonds and vibrations. In this article, we will discuss the characteristic peaks commonly observed in infrared spectroscopy and provide an infrared spectroscopy characteristic peak reference table.1. Aromatic C-H Stretching Vibrations: 3100-3020 cm^-1。
This region corresponds to the stretching vibrations of aromatic C-H bonds. The presence of peaks in this region indicates the presence of aromatic rings in the molecule.2. Alkyl C-C Stretching Vibrations: 1450-1375 cm^-1。
傅里叶红外光谱英文缩写The abbreviation for Fourier Transform Infrared Spectroscopy (FTIR).Fourier Transform Infrared Spectroscopy (FTIR) is a widely used analytical technique for the identification and characterization of organic and inorganic compounds. FTIR measures the absorption of infrared radiation by a sample, providing information about its chemical structure and composition. The technique is based on the principle that different chemical bonds absorb infrared radiation at specific frequencies, allowing for the identification of functional groups and molecular structure.FTIR has many applications in various fields, including pharmaceuticals, polymers, food and beverage, environmental analysis, and forensic science. In pharmaceuticals, FTIR is used to identify and quantify active ingredients and excipients in drug formulations, as well as to monitor the stability of pharmaceutical products. In the polymer industry, FTIR is used to analyze the composition and structure of polymer materials, such as plastics and rubbers, to ensure product quality and performance.In environmental analysis, FTIR is used to detect and identify pollutants in air, water, and soil, as well as to monitor the degradation of environmental contaminants. In forensic science, FTIR is used to analyze trace evidence, such as fibers, paints, and drugs, for criminal investigations.FTIR works by passing infrared radiation through a sample and measuring the absorbance of the radiation at different wavelengths. The resulting spectrum, called an infrared spectrum, provides a unique "fingerprint" of the sample's chemical composition. FTIR instruments can analyze samples in various physical forms, including liquids, solids, and gases, making it a versatile tool for different analytical needs.FTIR instruments can be equipped with additional accessories, such as attenuated total reflectance (ATR) or diffuse reflectance, to analyze samples without the needfor sample preparation. In addition, FTIR can be coupled with other analytical techniques, such as gas chromatography (GC-FTIR) or mass spectrometry (MS-FTIR), to provide complementary information about complex samples.FTIR has several advantages, including rapid analysis, high sensitivity, and non-destructive nature, making it an essential tool for quality control, research, and development in various industries. FTIR is also relatively easy to use, with modern instruments featuring user-friendly software for data acquisition and analysis.In conclusion, Fourier Transform Infrared Spectroscopy (FTIR) is a powerful analytical technique with diverse applications in pharmaceuticals, polymers, environmental analysis, and forensic science. Its ability to provide detailed information about chemical structure and composition makes it an indispensable tool for research, development, and quality control in various industries.傅里叶红外光谱英文缩写为FTIR。
兽医微生物学英语Veterinary Microbiology is a branch of microbiology that specifically focuses on the study of microorganisms that cause diseases in animals. This field is crucial for understanding and controlling infectious diseases in animals, which can also have implications for human health.One of the key areas of study in veterinary microbiology is the identification and characterization of pathogenic microorganisms. This involves isolating and culturing microorganisms from infected animals, and then using various techniques such as microscopy, biochemical tests, and molecular methods to identify and characterize the pathogens.Understanding the mechanisms of pathogenesis is another important aspect of veterinary microbiology. This involves studying how microorganisms cause disease in animals, including the role of virulence factors, host-pathogen interactions, and immune responses. By understanding these mechanisms, veterinarians can develop more effective strategies for preventing and treating infectious diseases in animals.Epidemiology is also a critical component of veterinary microbiology. This involves studying the distribution and spread of infectious diseases in animal populations, aswell as identifying risk factors and potential sources of infection. Epidemiological studies are essential for implementing control measures and preventing outbreaks of infectious diseases in animal populations.In addition to studying infectious diseases, veterinary microbiologists also play a key role in monitoring and controlling zoonotic diseases, which are diseases that can be transmitted from animals to humans. By understanding the epidemiology and transmission dynamics of zoonotic diseases, veterinarians can help prevent the spread of these diseases to humans and protect public health.In summary, veterinary microbiology is a diverse and essential field that encompasses the study of pathogenic microorganisms, mechanisms of pathogenesis, epidemiology, and zoonotic diseases. By advancing our understanding of infectious diseases in animals, veterinary microbiologists contribute to the health and well-being of both animals and humans.兽医微生物学是微生物学的一个分支,专门研究导致动物疾病的微生物。
精细化工规范标准规范文件英文回答:Fine chemical specification standards and regulations are essential for ensuring the quality and safety of fine chemical products. These standards provide guidelines and requirements for the manufacturing, testing, and use offine chemicals in various industries.One important aspect of fine chemical specification standards is the identification and characterization of the chemical substances. This includes providing detailed information about the chemical composition, purity, and physical properties of the fine chemicals. For example, the standard may specify the minimum purity level required for a particular chemical, or the acceptable range of impurities.Another crucial aspect is the safety and handling guidelines for fine chemicals. This includes information onthe proper storage, transportation, and disposal of the chemicals, as well as guidelines for handling and personal protective equipment. For instance, the standard may specify the appropriate storage conditions, such as temperature and humidity, to ensure the stability and integrity of the fine chemicals.Quality control and testing requirements are also an integral part of fine chemical specification standards. These standards outline the procedures and methods for testing the quality and performance of the fine chemicals. For example, the standard may require specific analytical techniques, such as gas chromatography or HPLC, to determine the purity and impurity profile of the chemicals.Furthermore, fine chemical specification standards also cover labeling and packaging requirements. This includes information on how the fine chemicals should be labeled, including the product name, chemical composition, hazard symbols, and safety warnings. The standards may also specify the type of packaging materials that should be used to ensure the stability and integrity of the chemicalsduring storage and transportation.Overall, fine chemical specification standards play a critical role in ensuring the quality, safety, and consistency of fine chemical products. These standards provide clear guidelines and requirements for manufacturers, users, and regulators, helping to promote the proper useand handling of fine chemicals across various industries.中文回答:精细化工规范标准规范文件对于确保精细化工产品的质量和安全至关重要。
clinical microbiology 检验英文原版书Title: Clinical Microbiology: An Essential Guide for Laboratory TestingIntroduction:Clinical microbiology plays a crucial role in the diagnosis and management of infectious diseases. It involves the laboratory testing of various specimens to identify and characterize microorganisms. In this article, we will explore the fundamental aspects of clinical microbiology as covered in the English original textbook.I. Overview of Clinical Microbiology:1.1 Importance of Clinical Microbiology:- Clinical microbiology aids in the identification of infectious agents, guiding treatment decisions.- It helps in the surveillance and monitoring of antimicrobial resistance.- It plays a vital role in outbreak investigations and infection control measures.1.2 Specimen Collection and Handling:- Proper collection and transportation of specimens ensure accurate and reliable results.- Different types of specimens, such as blood, urine, respiratory secretions, and wound swabs, require specific collection techniques.- Special considerations, such as timing and transport media, are essential to maintain the viability of microorganisms.1.3 Laboratory Techniques:- Microscopy techniques, including gram staining and acid-fast staining, allow for the visualization of microorganisms.- Culture-based methods involve the isolation and identification of microorganisms on various selective and differential media.- Molecular techniques, such as polymerase chain reaction (PCR), enable the detection and characterization of microorganisms at the genetic level.II. Bacterial Infections:2.1 Identification and Characterization:- Bacterial identification relies on phenotypic characteristics, including colony morphology, biochemical tests, and serological methods.- Advanced techniques, such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), provide rapid and accurate identification.- Antimicrobial susceptibility testing determines the susceptibility or resistance of bacterial isolates to specific antibiotics.2.2 Virulence Factors and Pathogenesis:- Understanding the virulence factors of bacteria helps in assessing their pathogenic potential.- Bacterial toxins, adhesins, and capsules contribute to the ability of bacteria to cause disease.- Molecular techniques, such as gene sequencing, aid in the identification and characterization of virulence factors.2.3 Antibiotic Resistance:- The emergence of antibiotic-resistant bacteria poses a significant challenge in clinical practice.- Mechanisms of antibiotic resistance include enzymatic inactivation, altered target sites, and efflux pumps.- Laboratory testing methods, such as disk diffusion and minimum inhibitory concentration (MIC) determination, help in monitoring and detecting antibiotic resistance.III. Viral Infections:3.1 Diagnostic Techniques:- Serological assays, such as enzyme-linked immunosorbent assay (ELISA), detect antibodies produced in response to viral infections.- Molecular methods, such as PCR and nucleic acid amplification tests (NAATs), directly detect viral genetic material.- Viral culture allows for the isolation and identification of viruses in specialized cell lines.3.2 Antiviral Therapy:- Laboratory testing helps in determining the susceptibility of viruses to antiviral drugs.- Drug resistance testing identifies mutations in viral genes associated with reduced drug effectiveness.- Viral load testing monitors the effectiveness of antiviral therapy by quantifying viral RNA or DNA.3.3 Emerging Viral Infections:- Clinical microbiology plays a crucial role in the detection and surveillance of emerging viral infections.- Rapid diagnostic tests, such as point-of-care assays, aid in the early identification of viral outbreaks.- Molecular techniques, such as next-generation sequencing (NGS), provide insights into the genetic diversity and evolution of emerging viruses.IV. Fungal and Parasitic Infections:4.1 Laboratory Diagnosis:- Direct microscopic examination of clinical specimens allows for the detection of fungal elements and parasites.- Culture-based methods help in the isolation and identification of fungal and parasitic organisms.- Molecular techniques, such as PCR and DNA sequencing, enhance the accuracy and speed of diagnosis.4.2 Antifungal and Antiparasitic Susceptibility Testing:- Laboratory testing determines the susceptibility or resistance of fungal and parasitic isolates to antifungal and antiparasitic drugs.- Methods, such as broth microdilution and agar dilution, provide quantitative susceptibility results.- Interpretive criteria assist in guiding treatment decisions based on the susceptibility profile of the organism.4.3 Epidemiology and Control Measures:- Clinical microbiology contributes to the surveillance and monitoring of fungal and parasitic infections.- Molecular typing techniques, such as multilocus sequence typing (MLST), aid in the investigation of outbreaks.- Infection control measures, including appropriate specimen handling and disinfection protocols, help prevent the spread of fungal and parasitic infections.V. Quality Assurance and Laboratory Management:5.1 Quality Control in Clinical Microbiology:- Quality control measures ensure the accuracy and reliability of laboratory results.- Internal quality control involves the use of known reference materials and regular monitoring of test performance.- External quality assessment programs provide proficiency testing to evaluate laboratory performance.5.2 Laboratory Safety:- Adherence to biosafety guidelines is essential to protect laboratory personnel and prevent laboratory-acquired infections.- Proper use of personal protective equipment, safe handling of infectious materials, and appropriate waste disposal are crucial aspects of laboratory safety.- Risk assessment and implementation of safety protocols minimize the potential for accidents and exposure to hazardous agents.5.3 Emerging Technologies and Future Perspectives:- Advances in technology, such as automation and artificial intelligence, are shaping the future of clinical microbiology.- Rapid diagnostic tests and point-of-care devices enable timely and accurate diagnosis at the patient's bedside.- Integration of big data analytics and genomics holds promise for personalized medicine and precision infectious disease management.Conclusion:Clinical microbiology is a multidisciplinary field that plays a vital role in the diagnosis, management, and prevention of infectious diseases. The English original textbook on clinical microbiology provides comprehensive coverage of the fundamental concepts, laboratory techniques, and practical applications in this field. By understanding the various aspects discussed in the book, healthcare professionals can enhance theirknowledge and skills in clinical microbiology, ultimately improving patient care and public health outcomes.。