甲状腺球蛋白ELISA试剂盒说明书甲状腺球蛋白ELISA试剂盒说明书检测范围96T40ng/L -1200ng/L甲状腺球蛋白ELISA试剂盒说明书使用目的本试剂盒用于测定人血清、血浆及相关液体样本中降钙素原（PCT）含量。
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甲状腺球蛋白ELISA试剂盒说明书Specimen requirements1 specimen collection as soon as possible to carry out the extraction, extraction according to the relevant literature, the extraction should be carried out as soon as possible after the experiment. If you can not immediately carry out the test, the specimen can be stored at -20 degrees C, but should avoid repeated freezing and thawing2 could not detect the NaN3 containing samples, because NaN3 inhibited the activity of horseradish peroxidase (HRP).Thyroglobulin protein ELISA reagent box manual operation step 2. Add: are respectively arranged in the blank hole (blank control wells without sample and ELISA, the rest of the each step of the operation is same), standard orifice, test sample hole. The enzyme labeled packet is in standard accurate sample of 50 UL, the tested sample hole Zhongxian sample dilution 40 g l, and then to be measured is added 10 mu l of sample (sample final dilution degrees for 5 times). The sample is added to the bottom of the enzyme labeled plate hole, as far as possible without touching the wall of the hole.3 temperature Education: with the sealing plate membrane sealing plate 37 degrees Celsius for 30 minutes.4 solution: 30 times the concentration of the washing liquid with distilled water 30 times diluted standby5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.6 enzymes: each hole is added to the enzyme labeled reagent 50 mu L, except the blank hole.7 Wen Yu: operation with 3.8 washing: operation with 5.9 Color: each hole to add the color agent A50 L, and then add the color agent B50 L, gently shake mix, 37 degrees to avoid light color 15 minutes.10 termination: 50 mu l per hole plus end solution, termination reaction (at this time blue, yellow).11: the determination of blank air zero absorbance at 450nm in order to measure the hole (OD). The determination should be carried out within 15 minutes after the addition of the liquid.Shanghai Joe feather Co., Ltd, ELISA kit, antibody, culture medium.AQP-3 ELISA kit calculationTo standard concentration as the abscissa, OD value as the ordinate, draw the standard curve on coordinate paper, according to the OD value of samples from the standard curve found corresponding concentration; multiplied by the dilution multiple; or with the standard concentration and the OD value calculated standard curve of the linear regression equation, the sample OD value in the equation, calculate the sample concentration, multiplied by the dilution factor is the actual concentration of the sample.AQP-3 ELISA kit notes1 kit from the cold storage environment should be removed at room temperature for 15-30 minutes after the use of the enzyme labeled package is not used up after the plate, the plate should be stored in a sealed bag.2 washing buffer will crystallization, heated the water solubilization dilution, washing does not affect the results.3 each step sample should be used, and often to check its accuracy, in order to avoid the test error. A sample within 5 mins, ifthenumberofsampleismuch, recommend to use volley like.4 each measurement and standard curve, it is best to do multiple holes. Such as samples to be measured matter content is too high (the sample od is bigger than the first standard hole hole OD), please first sample dilution multiples (n times) were measured and calculated please then multiplied by the total dilution ratio (n * * 5).5 closureplatemembrane only the disposable use, to avoid cross contamination.6 substrate please avoid light preservation.7 in strict accordance with the instructions of the operation, the results of the test must be determined in accordance with the enzyme standard instrument readings.8 all samples, washing liquid and all kinds of waste should be treated as infectious agents.9 different batches of this reagent can not be mixed.10 if there is a difference between the specification and the English specification.AQP-3 ELISA kit storageandvalidity1 Kit preservation: 2-8.2 validity: 6 months甲状腺球蛋白ELISA试剂盒说明书试剂盒组成1 30 倍浓缩洗涤液20ml×1 瓶；2 酶标试剂6ml×1 瓶3 酶标包被板12 孔×8 条；4 样品稀释液6ml×1 瓶5 显色剂A 液6ml×1 瓶；6 显色剂B 液6ml×1/瓶7 终止液6ml×1 瓶；8 标准品（48ng/ml）0.5ml×1 瓶9 标准品稀释液1.5ml×1 瓶；10 说明书1 份11 封板膜2 张；12 密封袋1甲状腺球蛋白ELISA试剂盒说明书本公司的产品只用于科研实验。
