MEGA6.06说明书
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HHAQ-1066-IN-1 Psychrometer With Enthalpy e-mail:**************For latest product manuals: Shop online at SM User’s Guide2The information contained in this document is believed to be correct, but OMEGA accepts no liability for any errors it contains, and reserves the right to alter specifications without notice.Servicing North America:U.S.A.: Omega Engineering, Inc., One Omega Drive, P.O. Box 4047 S tamford, CT 06907-0047 USA Toll-Free: 1-800-826-6342 (USA & Canada only) Customer Service: 1-800-622-2378 (USA & Canada only) Engineering Service: 1-800-872-9436 (USA & Canada only) Tel: (203) 359-1660 Fax: (203) 359-7700 e-mail:**************For Other Locations Visit /worldwide ***********************TABLE OF CONTENTS Introduction . . . . . . . . . . . . . . . . . . . . . 3 – 5 Key Features . . . . . . . . . . . . . . . . . . . . . . . 5 What’s in the Package . . . . . . . . . . . . . . . 6 Product Overview . . . . . . . . . . . . . . . . 6 – 8 Setup Instructions . . . . . . . . . . . . . . . . . . . 9 Install Batteries . . . . . . . . . . . . . . . . . . . 9 Operating Instructions . . . . . . . . . . . . 9 – 11 Specifications . . . . . . . . . . . . . . . . . . . . . 12 Operating & Maintenance Tips . . . . . . . . 13 INTRODUCTIONThank you for purchasing OMEGA’s HHAQ-106 6-in-1 Psychrometer with Enthalpy. Please read this user’s manual carefully and thoroughly before using the instrument.The HHAQ-106 can measure any environment’s ambient temperature, relative humidity (RH), dew point and wet bulb temperatures, absolute humidity (mixing ratio) and enthalpy of vaporization. These measurements are typically made by three groups of users:• Water damage restoration contractors • HVAC/R system installers and technicians3• P rofessionals charged with monitoring and maintaining the environment of facilities such as office buildings, greenhouses, food and equipment storage facilities, wineries, freezers, shipping containers, computer rooms, labs, libraries, museums and saunas.The dew point is the temperature below which the water vapor in a volume of air at a given constant barometric pressure will condense into liquid water at the same rate at which it evaporates. Condensed water is called dew when it forms on a solid surface. Another way to think of the dew point is as an air saturation temperature associated with relative humidity (RH). A high RH value indicates that the dew point is close to the current ambient air temperature. At 100% RH, the dew point temperature is equal to the ambient temperature because the air is completely saturated with water.The wet bulb temperature is the temperature that a volume of air would have if it were cooled to saturation (100% RH) by the evaporation of water into it, with the latent heat coming from the volume of air. It is the lowest temperature that can be reached under current ambient conditions by the evaporation of water only. The wet bulb temperature is the temperature you feel when your skin is wet and exposedto moving air as opposed to the actual air temperature—the dry bulb temperature.4An environment’s absolute humidity level (or mixing ratio) can be measured and expressed in units of grains per pound (GPP) or g/kg. GPP is a more useful moisture metric than RH to water damage remediators. Using RH alone, a remediator might unknowingly introduce moist air—with a low RH but a high GPP—during a job’s drying phase.The sixth environmental parameter thatthe HHAQ-106 can measure is enthalpy. Enthalpy is a measure of the amount of energy needed to change the state of a substance from a solid to a liquid or from a liquid to a gas. The most common application for the HHAQ-106’s enthalpy-calculating algorithm is measuring the enthalpy of vaporization of air in an HVAC/R system duct. The HHAQ-106 is powered by (3) “AAA” batteries (included).KEY FEATURES• I ntegral 6 in. long sensor-tipped probe for measuring conditions inside ductwork. Probe swivels, increasing placement options.• C hoice of Imperial or metric units for all measured parameters except RH• 4-digit LCD• 2-minute Auto Power Off (APO) function • L ow battery indicator5WHAT’S IN THE PACKAGE The HHAQ-106 comes fully assembled in a blister pack along with a rubber bushing for inserting the probe into ductwork, (3) “AAA” batteries and this user’s manual. PRODUCT OVERVIEW Figure 1 shows all of the controls, indicators and physical structures on the front and back of the HHAQ-106. Figure 2 showsall text and icons that could appear onthe unit’s LCD. Familiarize yourself with the positions and functions of all buttons, structures and icons before moving onto the Setup Instructions and Operating Instructions.67B FA D C EGFig. 1. The HHAQ-106’s controls, indicators and physical structuresA. H umidity and temperature sensors under protective capB. LCDC. MODE buttonD. UNIT buttonE. Pocket clipF. Battery compartmentG. R ubber bushing for inserting probe in ductworkFig. 2. A ll possible display indications and their meaningsRH = Relative humidity mode indicator TEMP = Temperature mode indicator DEW = Dew point mode indicatorWET = Wet bulb mode indicatorMIX = Mixing ratio mode indicator ENTH = Enthalpy mode indicatorGPP = Mixing ratio Imperial unitg/kg = Mixing ratio metric unitkJ/kg = Enthalpy metric unitBTU/lb = Enthalpy Imperial unit°F = Fahrenheit indicator°C = Celsius indicator= Low battery indicator8SETUP INSTRUCTIONS INSTALL BATTERIES1. T he meter’s battery compartment (Fig. 1, Callout F) is accessible from the bottom of the unit. Slide the battery compartment cover down and away from the HHAQ-106 and set it aside.2. I nsert the three supplied “AAA” batteries in the compartment, orienting them as shown on the diagram stenciled on the battery compartment cover3. R eplace the battery compartment cover and slide it up until it latches shut. OPERATING INSTRUCTIONSBefore making any measurements, rotate the sensor protection cap (Fig. 1, Callout A) with your thumb and index finger so both of its vents are open to the ambient environment. Press the MODE buttonto power on the meter. The LCD will immediately begin reading out the RH value of the environment. The reading will be accompanied by the term RH in the upper left corner of the LCD.To display the ambient temperature, press the MODE button once. TEMP will replace RH on the top line of the LCD.9To display the dew point temperature, press the MODE button again. DEW will replace TEMP on the top line of the LCD.To display the wet bulb temperature, press the MODE button again. DEW will disappear from the top line of the LCD and be replaced by WET on the bottom line.To display the mixing ratio (absolute humidity) of the environment, press the MODE button again. MIX will replace WET on the bottom line of the LCD.To display the enthalpy of the environment, press the MODE button again. ENTH will replace MIX on the bottom line of the LCD.How pressing the MODE button changesthe measured parameterFor all parameters except RH, the HHAQ-106 will automatically display readingsin Imperial units. To switch to metric units for ambient, dew point or wet bulb temperature, mixing ratio or enthalpy, press 10the UNIT button. To toggle back to Imperial units, press the UNIT button again.The placement of the HHAQ-106’s humidity and temperature sensors at the end of a probe makes it possible to take readings inside ductwork. And the fact that the probe swivels increases positioning options if the probe is inserted through a vent or grille.If you wish to measure duct conditions where there is no grille or duct nearby, consider drilling a temporary hole in the ductwork and inserting the probe through it. The black rubber bushing is provided for this purpose. It is sized for a 1 in. diameter hole. Inserting the probe through it will extend the sensor 4 in. into the duct.To extend the life of the batteries powering the HHAQ-106, the unit’s Auto Power Off (APO) function will automatically shut down the instrument if no front-panel buttons are pressed within any 2-minute period.To power off the HHAQ-106, press and hold the MODE button for at least 3 seconds.11SPECIFICATIONS Ambient Temperature Measurement Range: -4° to 158°F (-20° to 70°C) Ambient Temperature Measurement Accuracy: 1.8°F (±1°C) Temperature Measurement Resolution: 0.1°RH Measurement Range: 0 to 99.9% RH Measurement Accuracy:±2% from 10% to 90%RH;±3% elsewhereRH Measurement Resolution: 0.1% Probe Length: 6 in. (150mm)Display Size: 1.5 in. (38mm) diagonal Current Consumption: <10mA Battery Life: 1000 hours (typical) Dimensions: 8.23 x 1.85 x 1.85 in. (209 x 47 x 47mm)Weight (without battery): 2.2 oz. (63g) Power Source: (3) “AAA” batteries (included)12OPERATING & MAINTENANCE TIPSWhen the icon appears on the top line of the display, it’s time to replace the batteries that power the instrument (although measurements will remain valid for several hours after the icon first appears). To replace the batteries, follow the instructions on page 9.To prevent dust and moisture from degrading the performance of the humidity and temperature sensors, after each measurement session rotate the sensor protection cap to close its three vents.Do not operate the HHAQ-106 in the presence of a flammable or explosive gas or near an arc welder or induction heater. After subjecting the unit to a large change in ambient temperature, wait at least30 minutes before making measurements to guarantee the accuracy of readings. Remove the batteries when storing the unit or when you do not expect to use it for an extended period of time (months rather than weeks).Do not drop or disassemble the HHAQ-106 or immerse it in water.13NOTES________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ ________________________________ 1415WARRANTY/DISCLAIMER OMEGA ENGINEERING, INC. warrants this unit to be free of defects in materials and workmanship for a period of 13 months from date of purchase. OMEGA’s WARRANT Y adds an additional one (1) month grace period to the normal one (1) year product warranty to cover handling and shipping time. This ensures that OMEGA’s customers receive maximum coverage on each product. If the unit malfunctions, it must be returned to the factory for evaluation. OMEGA’s Customer Service Department will issue an Authorized Return (AR) number immediately upon phone or written request. Upon examination by OMEGA, if the unit is found to be defective, it will be repaired or replaced at no charge. OMEGA’s WARRANT Y does not apply to defects resulting from any action of the purchaser, including but not limited to mishandling, improper interfacing, operation outside of design limits, improper repair, or unauthorized modification. T his WARRANT Y is VOID if the unit shows evidence of having been tampered with or shows evidence of having been damaged as a result of excessive corrosion; or current, heat, moisture or vibration; improper specification; misapplication; misuse or other operating conditions outside of OMEGA’s control. Components in which wear is not warranted, include but are not limited to contact points, fuses, and triacs.OMEGA is pleased to offer suggestions on the use of its various products. However, OMEGA neither assumes responsibility for any omissions or errors nor assumes liability for any damages that result from the use of its products in accordance with information provided by OMEGA, either verbal or written. OMEGA warrants only that the parts manufactured by the company will be as specified and free of defects. OMEGA MAKES NO OTHER W ARRANTIES OR REPRESENTATIONS OF ANY KIND W HATSOEVER, EXPRESSED OR IMPLIED, EXCEPT THAT OF TITLE, AND ALL IMPLIED WARRANTIES INCLUDING ANY W ARRANTY OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE HEREBY DISCLAIMED. LIMITATION OF LIABILITY: The remedies of purchaser set forth herein are exclusive, and the total liability of OMEGA with respect to this order, whether based on contract, warranty, negligence, indemnification, strict liability or otherwise, shall not exceed the purchase price of the component upon which liability is based. In no event shall OMEGA be liable for consequential, incidental or special damages.CONDITIONS: Equipment sold by OMEGA is not intended to be used, nor shall it be used: (1) as a “Basic Component” under 10 CFR 21 (NRC), used in or with any nuclear installation or activity; or (2) in medical applications or used on humans. Should any Product(s) be used in or with any nuclear installation or activity, medical application, used on humans, or misused in any way, OMEGA assumes no responsibility as set forth in our basic WARRANT Y / DISCLAIMER language, and, additionally, purchaser will indemnify OMEGA and hold OMEGA harmless from any liability or damage whatsoever arising out of the use of the Product(s) in such a manner.OMEGA’s policy is to make running changes, not model changes, whenever an improvement is possible. This affords our customers the latest in technology and engineering.OMEGA is a registered trademark of OMEGA ENGINEERING, INC.© Copyright 2015 OMEGA ENGINEERING, INC. All rights reserved. This document may not be copied, photocopied, reproduced, translated, or reduced to any electronic medium or machine-readable form, in whole or in part, without the prior written consent of OMEGA ENGINEERING, INC.FOR WARRANTY RETURNS, please have the following information available BEFORE contacting OMEGA:1. P urchase Order number under which the product was PURCHASED,2. M odel and serial number of the product under warranty, and 3. R epair instructions and/or specific problems relative to the product.FOR NON-WARRANTY REPAIRS, consult OMEGA for current repair charges. Have the following information available BEFORE contacting OMEGA:1. P urchase Order number to cover the COST of the repair,2. M odel and serial number of the product, and 3. R epair instructions and/or specific problems relative to the product.RETURN REQUESTS / INQUIRIES Direct all warranty and repair requests/inquiries to the OMEGA Customer Service Department. BEFORE RET URNING ANY PRODUCT (S) T O OMEGA, PURCHASER MUST OBT AIN AN AUT HORIZED RET URN (AR) NUMBER FROM OMEGA’S CUST OMER SERVICE DEPART MENT (IN ORDER T O AVOID PROCESSING DELAYS). T he assigned AR number should then be marked on the outside of the return package and on any correspondence.T he purchaser is responsible for shipping charges, freight, insurance and proper packaging to prevent breakage in transit.Where Do I Find Everything I Need for Process Measurement and Control?OMEGA…Of Course!Shop online at SMTEMPERATUREM U T hermocouple, RTD & Thermistor Probes,Connectors, Panels & AssembliesM U Wire: Thermocouple, RTD & ThermistorM U Calibrators & Ice Point ReferencesM U Recorders, Controllers & Process MonitorsM U Infrared PyrometersPRESSURE, STRAIN AND FORCEM U Transducers & Strain GagesM U Load Cells & Pressure GagesM U Displacement TransducersM U Instrumentation & AccessoriesFLOW/LEVELM U Rotameters, Gas Mass Flowmeters & Flow ComputersM U Air Velocity IndicatorsM U Turbine/Paddlewheel SystemsM U Totalizers & Batch ControllerspH/CONDUCTIVITYM U pH Electrodes, Testers & AccessoriesM U Benchtop/Laboratory MetersM U Controllers, Calibrators, Simulators & PumpsM U Industrial pH & Conductivity EquipmentDATA ACQUISITIONM U Data Acquisition & Engineering SoftwareM U Communications-Based Acquisition SystemsM U Plug-in Cards for Apple, IBM & CompatiblesM U Data Logging SystemsM U Recorders, Printers & PlottersHEATERSM U Heating CableM U Cartridge & Strip HeatersM U Immersion & Band HeatersM U Flexible HeatersM U Laboratory HeatersENVIRONMENTALMONITORING AND CONTROLM U Metering & Control InstrumentationM U RefractometersM U Pumps & TubingM U Air, Soil & Water MonitorsM U Industrial Water & Wastewater TreatmentM U pH, Conductivity & Dissolved Oxygen InstrumentsM5492/0815。
Megavideo-NH061中文说明书Megavideo美佳威迪欧MPV-061C高清CCD百万像素网络摄像机使用手册V1.0Megavideo1TableofContents1.概览overview (3)1.1GeneralFeatures................................................................................. .. (3)1.2产品包装Packagecontents.............................................................................................. . (4)2.网络摄像机介绍introductionofIPcamera (5)2.1摄像机尺寸Dimensions........................................................................................................ . (5)2.2后背板连接口定义:connectorsontherearboard..................................................................... . (5)3.安装网络摄像机PreparationofIPcamerasetup (7)3.1系统需求SystemRequirements...................................................................................... .. (7)3.2摄像机安装说明........................................................................................................................... .. (7)3.3IP摄像机初步联机AccessingIPCamera......................................................................................... . (9)4.配置与操作Configuration&Operation (15)4.1IE浏览器介绍(InternetExplorer)............................................................................................. . (15)4.2主页HomePage......................................................................................................... (16)4.3系统相关设置SystemRelatedSettings.................................................................................. .. (18)4.3.1主机名和系统时间设定HostNameandSystemTimeSetting (19)4.3.2安全性Security................................................................................................................ (20)4.3.3网络Network.............................................................................................................. .. (22)4.3.4动态域名解析服务DDNS (25)4.3.5电邮功能Mail....................................................................................................................... (27)4.3.6FTP....................................................................................................... . (27)4.3.7应用Application......................................................................................................... .. (28)4.3.8位移侦测MotionDetection............................................................................................. .. (33)4.3.9快照Snapshot............................................................................................................. .. (37)4.3.10焦点校正IrisAdjustment.................................................................................................. . (38)4.3.11查看Log文檔ViewLogFile........................................................................................................384.3.12查看用户信息ViewUserInformation..................................................................................... .. (40)4.3.13查看属性FactoryDefault.................................................................................................. (42)4.3.14回复工厂默认值FactoryDefault..................................................................................................424.3.15软件版本SoftwareVersion............................................................................................... . (44)4.3.16软件升级SoftwareUpgrade............................................................................................ .. (45)4.4视频和音频流设置VideoandAudioStreamingSettings............................................................ .. (47)4.4.1视频分辨率和旋转型VideoResolutionandRotateType (4)74.4.2视频压缩比VideoCompression.......................................................................................... . (49)4.4.3视频OCX的议定书VideoOCXProtocol (51)4.4.4视频跳张设定VideoFrameSkip............................................................................................... . (53)4.4.5VideoMask....................................................................................... . (54)Megavideo24.4.6音频模式和比特率设置AudioModeandBitRateSettings (54)4.5摄像机设置CameraSettings................................................................................................ . (57)4.5.1曝光设定ExposureSetting............................................................................................... .. (58)4.5.2白平衡设置WhiteBalanceSetting...................................................................................... . (59)4.5.3背光设置BacklightSetting............................................................................................... . (59)4.5.4亮度设置BrightnessSetting............................................................................................ (60)4.5.5锐利度设置SharpnessSetting............................................................................................. .. (60)4.5.6对比度设置ContrastSetting................................................................................................ (60)4.5.7数位变焦设置DigitalZoomSetting........................................................................................ (60)4.5.8宽动态功能WDRFunction.................................................................................................... . (60)4.5.9三维降低噪声功能3DNRFunction (6)14.5.10电视系统设置TVSystemSetup................................................................................................ . (61)附录A:技术规格 (63)附录B:互联网安全设置 (65)附录C:DCViewer下载步骤 (69)附录D:背焦调整BackFocusAdjustment (73)Megavideo31.概览overview高清的百万像素宽动态网络摄像机,提供最佳的图像质量。
SLC7A11基因在不同毛色的羊驼皮肤组织中表达分析摘要:SLC7A11基因是编码溶质载体7家族的第11个成员,已经被证实是影响头发和黑色素细胞中黑色素合成的关键调控基因。
为了更好的了解SLC7A11基因在白色与棕色的羊驼中的表达模式,我们克隆了羊驼SLC7A11基因全编码序列(CDS),利用实时荧光定量PCR技术,免疫印迹试验和免疫组织化学法来分析表达模式。
全编码序列(CDS)有1512个碱基对,编码503个氨基酸多肽。
序列分析表明,和脊椎动物编码区相似,羊驼xCT包含12个跨膜区域的高度保守的氨基酸通透酶(AA_permease_2)。
序列比对和系统发育分析显示,羊驼xCT和野骆驼同一性最高并共享相同的分支。
实时定量荧光PCR 和免疫印迹试验结果显示xCT在棕色的羊驼皮肤水平明显很高,而在白色和棕色的羊驼皮肤转录和蛋白表达具有相同的表达水平。
此外,免疫组织化学分析进一步表明,棕色的羊驼与白色相比xCT在基质和根鞘的染色稳定性提高。
这些结果表明,SLC7A11基因有调节羊驼毛色的功能,为进一步探索SLC7A11基因对黑色素合成的作用提供了必要的信息。
关键词:SLC7A11基因;xCT;羊驼;毛色;皮肤引言在成年动物中,头发和皮肤的颜色都是由位于皮肤表皮下的黑色素细胞产生的色素决定的。
在哺乳动物皮肤和鸟类黑色素细胞中发现产生两种不同类型的黑色素,一种是表现黑色到褐色的真黑色素,还有一种是表现出黄色到红棕色的褐黑素。
真黑素和褐黑素的质量和比例改变着色素沉着而导致皮肤、羊毛、羽毛和眼睛虹膜的颜色改变。
目前的研究表明,大量的基因能调节人类和脊椎动物的头发和皮肤的颜色。
但是调节毛用山羊表皮颜色的分子和细胞机制尚未完全阐明。
羊驼有种类繁多的自然毛色,但这种毛色的遗传和生物调控机制仍不清楚。
研究人员先前通过对不同毛色的羊驼的黑素皮质素受体1蛋白多态性(MC1R)和刺鼠信号蛋白(ASIP)研究,推测这些基因的多态性可能会改变毛色。
坛紫菜6-磷酸海藻糖合成酶(TPS)家族基因的克隆及表达特征分析史健志;徐燕;纪德华;陈昌生;谢潮添【摘要】6-磷酸海藻糖合成酶(TPS)是植物海藻糖合成的关键酶,在植物逆境胁迫应答中发挥着重要的作用.实验以坛紫菜转录组测序获得的unigene序列为基础,采用RACE技术克隆获得坛紫菜的3条TPS基因序列:PhTPS1,PhTPS2-1和PhTPS2-2.序列分析结果表明,PhTPS1(收录号:KM580358)序列全长3 557 bp,包含一个3 462 bp的开放阅读框,所编码的多肽包含1 153个氨基酸,分子量为124.2 ku,等电点为6.73,属于TPS Ⅰ亚家族;PhTPS2-1(收录号:KM519457)序列全长4 264 bp,包含一个4 044 bp的开放阅读框,所编码的多肽包含1 374个氨基酸,分子量为145.2 ku,等电点为5.96;PhTPS2-2(收录号:KM519458)序列全长3 733 bp,包含一个3 324 bp的开放阅读框,所编码的多肽包含1 107个氨基酸,分子量为120.2 ku,等电点为5.42.PhTPS2-1和PhTPS2-2属于TPSⅡ亚家族.基因表达水平的定量分析结果表明高温胁迫条件下,3条PhTPS基因的表达模式基本一致,均表现为先上调后下调,再上调的趋势;而PhTPS1和PhTPS2-1基因在中低水平的失水条件下,基因表达水平均没有发生显著变化,只在高水平失水胁迫下显著上调,PhTPS2-2基因在中高水平失水条件下,表达水平显著上调.说明PhTPS基因可能只在高度失水胁迫下发挥应激调节作用.【期刊名称】《水产学报》【年(卷),期】2015(039)004【总页数】11页(P485-495)【关键词】坛紫菜;6-磷酸海藻糖合成酶;RACE;荧光定量PCR【作者】史健志;徐燕;纪德华;陈昌生;谢潮添【作者单位】集美大学水产学院,福建厦门 361021;集美大学水产学院,福建厦门361021;集美大学水产学院,福建厦门 361021;集美大学水产学院,福建厦门361021;集美大学水产学院,福建厦门 361021【正文语种】中文【中图分类】Q786;S968.4海藻糖(trehalose)是一种广泛存在于生物界中,由两个吡喃环葡萄糖分子通过α-1,1糖苷键连接而成的非还原性双糖,是天然双糖中理化性质最为稳定的一类糖质[1]。
Supertex inc.VN0606YY = Year Sealed WW = Week Sealed= “Green” PackagingSi VN 0606L YYWW Features►Free from secondary breakdown ►Low power drive requirement ►Ease of paralleling►Low C ISS and fast switching speeds ►Excellent thermal stability ►Integral source-drain diode►High input impedance and high gainApplications►Motor controls ►Converters ►Amplifiers ►Switches►Power supply circuits►Drivers (relays, hammers, solenoids, lamps, memories, displays, bipolar transistors, etc.)General DescriptionThis enhancement-mode (normally-off) transistor utilizes a vertical DMOS structure and Supertex’s well-proven, silicon-gate manufacturing process. This combination produces a device with the power handling capabilities of bipolar transistors and the high input impedance and positive temperature coefficient inherent in MOS devices. Characteristic of all MOS structures, this device is free from thermal runaway and thermally-induced secondary breakdown.Supertex’s vertical DMOS FETs are ideally suited to a wide range of switching and amplifying applications where very low threshold voltage, high breakdown voltage, high input impedance, low input capacitance, and fast switching speeds are desired.N-Channel Enhancement-ModeVertical DMOS FEToccur. Functional operation under these conditions is not implied. Continuous operation of the device at the absolute rating level may affect device reliability. All voltages are referenced to device ground.TO-92Product MarkingPackage may or may not include the following marks: Si orPin ConfigurationTO-92GATESOURCEDRAINContact factory for Wafer / Die availablity.Devices in Wafer / Die form are lead (Pb)-free / RoHS compliant.OSwitching Waveforms and Test CircuitOUTPUTINPUTOUTPUT10VVDD0V0V1. All D.C. parameters 100% tested at 25O C unless otherwise stated. (Pulse test: 300µs pulse, 2% duty cycle.)2. All A.C. parameters sample tested.† I D (continuous) is limited by max rated T j .Supertex inc. does not recommend the use of its products in life support applications, and will not knowingly sell them for use in such applications unless it receives an adequate “product liability indemnification insurance agreement.” Supertex inc. does not assume responsibility for use of devices described, and limits its liability to the replacement of the devices determined defective due to workmanship. No responsibility is assumed for possible omissions and inaccuracies. Circuitry and specifications are subject to change without notice. For the latest product specifications refer to the Supertex inc. (website: http//)©2013 Supertex inc. All rights reserved. Unauthorized use or reproduction is prohibited.Supertex inc.(The package drawing(s) in this data sheet may not reflect the most current specifications. For the latest package outline information go to /packaging.html .)3-Lead TO-92 Package Outline (L)JEDEC Registration TO-92.* This dimension is not specified in the JEDEC drawing.† This dimension differs from the JEDEC drawing.Drawings not to scale.Supertex Doc.#: DSPD-3TO92N3, Version E041009.。
第一步:打开软件下面介绍菜单的使用:Data菜单:Creat a new :创建一个新的数据比对文件,也就是说当我们比对完一组后,想接着比对另一组,那么使用它就可以不用退出直接把数据文件导入;Open :打开先前已经比对并保存好的文件,它包含两个子菜单:retive sequence from file 和saved aligment session ;Close: 关闭当前的比对数据文件;Save session :保存当前比对结果,可以给比对的结果一个文件名;Export alignment :将当前的序列比对结果输出到指定文件,有两种输入格式可供选择:MGTA 和FASTA.DNA sequence :使用它来选择输入的数据DNA 序列,这里需要说明的是如果你输入的数据是氨基酸序列的话,比对窗口只显示一个标签,若是DNA 序列的话则显示两个标签,一个是DNA 序列的,另一个是氨基酸序列的。
Protein sequences :选择输入的氨基酸序列,选择后,所以的位点就被当作氨基酸残基位点来对待。
Translate/untranslate :只有比对的序列是编码蛋白的DNA序列的时候才可用。
它可以根据指定的遗传密码表将DNA 序列翻译成特定的氨基酸序列。
Select genetic code table :使用它将编码蛋白的DNA 翻译成特定的蛋白序列。
R everse complement :将选择的一整行的DNA 序列变为与之互补配对碱基序列。
Exit alignment explorer :退出序列比对的资源管理窗口Edit 菜单:使用这个菜单可以对我们的比对序列进行想要的一些编辑工作具体为Undo:撤销上一步操作;Copy:复制;Cut:剪切;Paste:粘贴;这三个操作都可以只针对一个碱基或氨基酸残基也可以是一段甚至是整个序列;Delete:从比对表格中删除一段序列;Delete gaps:去掉序列中的空缺;Insert blank sequence:重新插入一空行;标签和序列都是空的;Insert sequence from file :从已保存的文件中插入新的序列;Select sites :选择一列序列,与点击比对表上方的灰白空格作用类似;Select sequence:选择一行序列,与点击比对表格左侧的标签名作用类似;Select all:全选;Allow base editing :只读保护,只有选择后才能对序列进行编辑操作,否则所以的序列为只读格式,不能进行任何编辑操作。
植物科学领域最全的“⽅法步骤软件”该⽹盘所有内容也将根据⼤家的补充逐渐更新⽅法步骤1Western_blot_protocol.pdf2Yeast transformation tips.pdf3标签蛋⽩简单介绍.pdf4采⽤本⽒烟草瞬时表达系统进⾏蛋⽩质免疫共沉淀实验 Protein Immuno.. (2).pdf5超级感受态制备.pdf6超级感受态制作⽅法ULTRA.pdf7从纯化到星⾠.pdf8蛋⽩质电泳相关试剂、缓冲液的配制⽅法 - 副本.pdf9分⼦克隆实验指南第三版上册.pdf10分⼦克隆实验指南第三版下册.pdf11分⼦克隆实验指南上.pdf12分⼦⽣物学实验技术简册.pdf13分⼦⽣物学实验室⼯作⼿册.pdf14国内重点实验室分⼦⽣物学实验⽅法汇总实验室常⽤实验⽅法.pdf15核酸电泳相关试剂、缓冲液的配制⽅法 - 副本.pdf16核酸电泳相关试剂、缓冲液的配制⽅法.pdf17核算蛋⽩互算.pdf18基因northern blot⽅法.pdf19基因X 中⽂版.pdf20基因⼯程原理(吴乃虎编著).pdf21基因芯⽚⾼质量-CTAB提DNA.pdf22基于Gateway体系⼀步PCR法构建CRISPR终载体.pdf23酵母单杂说明书.pdf24酵母⾼效转化⽅法.pdf25酵母双杂交操作.pdf26酵母双杂交技术 - 副本.pdf27酵母相关实验⽅法.pdf28芥菜幼苗中超氧化物和H2O2累积的组织化学检测.pdf29抗⽣素的贮存溶液及其⼯作浓度 - 副本.pdf30类胡萝⼘素测定-Functional analysis of beta- and epsilon-ring carotenoid hydroxylases in Arabidopsis.pdf31免疫共沉淀Co-IP实验操作步骤.pdf32拟南芥的实验室⼿册.pdf33拟南芥提DNA.pdf34拟南芥叶⽚过氧化氢DAB染⾊.pdf35凝胶迁移实验(EMSA).pdf36农杆菌注射烟草叶⽚进⾏体内瞬时表达的⽅法.pdf37染⾊质免疫共沉淀(ChIP)实验.pdf38如何使⽤DNAMAN软件制作序列⽐对.pdf39实时荧光pcr中⽂.pdf40实时荧光定量PCR全⽅位解析.pdf41实验室常⽤培养基的配制⽅法 - 副本.pdf42实验室常⽤培养基的配制⽅法.pdf43实验室常⽤试剂、缓冲液的配制⽅法 - 副本.pdf44⼿把⼿教你使⽤EndNote_X8.pdf45数量遗传学、基因组学和植物分⼦育种.pdf46双酶切连接反应之全攻略.pdf47⽔稻多基因敲除系统.pdf48⽔稻原⽣质体的分离转化.pdf49体外降解.pdf50⽂献综述的写法详细.pdf51现代分⼦⽣物学第4版_朱⽟贤2013.pdf52原位杂交procotol.pdf53植物病原体感染期间的植物组织台盼蓝染⾊Bio-protocol2078.pdf 54植物组织电镜基本制样⽅法20171117.pdf55转基因⼤麦中gfp基因的染⾊体位置及其表达.pdf56转录组分析软件上机指南.pdf57综述的写法.pdf58总-常⽤逆境⽣理指标测定⽅法-LCL.pdf59[]毕⾚酵母表达操作⼿册(精译版).pdf609页的操作yeast Transformation.pdf612003 pMDC100 pDMC32 A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta.pdf622007 pMDC100 pDMC32 Recombinational Cloning with Plant Gateway Vectors.pdf 63630491 中⽂版-单杂载体说明.pdf64 A Foxtail mosaic virus Vector for Virus-Induced Gene silencing in maize.pdf65 a loop-mediated isothermal amplification method.pdf66 A Toolkit for Illustrating Heatmap.PDF67Alexander_staining(⼀种花粉活性染⾊法).pdf68arabidopsis mesophyll bifc.pdf69Arabidopsis mesophyll protoplasts.pdf70BiFC.pdf71Bio-Rad荧光定量PCR应⽤指南.pdf72bp_clonaseii_man.pdf73CAPS_and_dCAPS.pdf74CapsaicinHPLC-UV-MS-PP.pdf75DUAL?Membrane?System?Protocol.pdf76EndNote.pdf77FISH Protocols.pdf78GAPDH酶活测定.pdf79Gateway Protocol.pdf80gateway system mannual.pdf81gateway_pdonr_vectors.pdf82GenStat统计⽅法与数据分析(1).pdf83GUS染⾊.pdf84GUS染⾊-杜长青.pdf85GV3101电击.pdf86HiTail PCR BTN_A_000112601_O_1421a.pdf87imageJ使⽤⽅法-叶⽚⾯积统计.pdf88ImpGWB manual E (pGWBnxx).pdf89infusion protocol.pdf90JoinMapML作图⽅法.pdf91Khalid Meksem, Guenter Kahl-The Handbook of Plant Genome Mapping_ Genetic and Physical Mapping (v. 1)-Wiley-VCH (2005).pdf92KOD –Plus- Neo (KOD-401).pdf93KOD-401.pdf94lr_clonase_man.pdf95LSM brief manual_final.pdf96Matchmaker Gold Yeast Two-Hybrid System User Manual (PT4084-1)_092413.pdf 97Maxent简明教程(翻译).pdf98MEGA3.1 中⽂使⽤说明.pdf99miRNA northern blot ⽅法.pdf100Molecular Plant Pathology.pdf101MultiExperiment_Viewer_Quickstart_Guide_MeV软件教程-1.pdf102Oligo的使⽤⽅法介绍.PDF103One-step enzymatic assembly of DNA-YubingHe.pdf104PCR Mutation Detection Protocols.pdf105Pcr Protocols-Methods In Molecular Biology.pdf106pDONR201.pdf107pDONR201 207 info..pdf108pgwb pdf.pdf109plant metabolite protocol and method.pdf110PrimeSTAR - 副本.pdf111R for Beginners 中⽂版.pdf112R050A PrimeSTAR? GXL DNA Polymerase.pdf113Rice Protocols_Methods in Molecular Biology, 2013, Vol. 956(Non-commercial use permitted).pdf114ROS染⾊⽅法.pdf115RPA III Kit.pdf116SDS PAGE.pdf117SPSS与多元统计分析⽅法讲义.pdf118Tai Te Wu Analytical Molecular Biology .pdf 119TAK-101_201.pdf120Vector_NTI_简体中⽂使⽤教程PDF版本.pdf 121Western blot详细操作步骤.pdf122western_blot - 副本.pdf123实验protocol⼤全124VIGS125endnote x8破解.rar.xltd.cfg126Endnote_X7实⽤教程.pptx127ij.jar128mmexport1510878447197.jpg129PastedGraphic-1.png130烟草遗传转化.jpg131可溶性糖和可溶性蛋⽩测定⽅案.wps132Adobe Acrobat XI Pro 11.0.1.rar.xltd.cfg 133SeqScannerInstall.exe.重命名134蛋⽩的相互作⽤ - 副本.ppt135蛋⽩质结构与功能预测.ppt136实验技术.zip.xltd.cfg137⽔稻花药花粉发育过程.jpg138注册账号.txt139载体构建.ppt140科研论⽂作图141⽟⽶营养液.xlsx142LI6400143protoplast_workshop.wmv144SPSS介绍与应⽤.pptx145⽂献管理教学-zd.pptx146拟南芥蜡质测定⽅法.jpg147软件148国家基础地理信息系统数据.zip149分⼦⽣物学150克隆构建载体知识积累151植物实验⽅法152转录组培训资料153补充的软件和试验⽅法154酵母双杂155实验技术156原⽣质体制备157备份word158⼦叶节培养程序2008整理.docx1594.5h Immunolocalization .doc160Arlequin操作说明.doc161change solution on August 2nc.doc162ChIP for Arabidopsis.doc163CHIP protocol.doc164CHIP实验⽅法.doc165CHIP实验⽅法2.doc166co-IP in vivo.doc167CTAB 提取DNA.doc168CTAB-96孔法⽔稻基因组DNA提取.doc169CTAB法提前DNA及其检测.doc170DNA粗提.doc171DNA提取过程中各种试剂的作⽤.doc172EMSA实验步骤.doc173FISH protocol.DOC174FISH药品配置.doc175H2O2的测定-KI法.doc176Hydrogen proxide_CN.doc177IAA treatment used for detecting IAA induced genes.doc 178In Situ hybridization in plants- protocol.doc179IPCR确定T-DNA突变体插⼊位点 - 副本.doc180Leica⽯蜡切⽚机操作规程.doc181LYM基因克隆构建PAbAi载体.doc182LYM酵母单杂流程.doc183Megazyme_总淀粉测定试剂盒翻译.doc184MS母液配制⽅法及⽤量.doc185MS培养基及配制注意事项 - 副本.doc186NEB mRNA 建库protocol.doc187N-P-K联合消煮测定.doc188PAGE胶流程.doc189PFP PFK ADH PDC enzyme activities.doc190POD 电泳⽅法.doc191Rice Leaf Protoplast Protocol-Modified by WM.doc192RT-PCR.doc193RuBP-PEP-Pr测定.doc194SEM.doc195SOD 电泳⽅法.doc196SOD、POD、CAT、MDA和可溶性蛋⽩测定.doc197VIGS实验⽅案.doc198WB实验步骤.doc199Y1H.doc200yongjiang氮代谢酶.doc201矮牵⽜转化.doc202半薄切⽚.doc203曹建康-果蔬采后⽣理⽣化实验指导.doc204测酶时需要配的溶液.doc205常⽤试剂配⽅.doc206超级感受态制备 (2).doc207超级感受态制备.doc208成熟胚⾼效转基因系统的建⽴(Ver_2011.03.21).doc209⼤肠杆菌电击感受态制备⽅法-曹⾼燚.doc210蛋⽩各组分含量测定⽅法.doc211蛋⽩酶活性的测定⽅法.doc212蛋⽩透析复性⽅法.doc213蛋⽩质胶内酶解及同位素标记实验步骤.doc214蛋⽩组学实验流程.doc215分⼦克隆技术实验操作⼿册2005.doc216分⼦⽣物学实验技术实验操作指南.doc217柑橘原⽣质体提取及转化.doc218根系活⼒测定-NRA测定.doc219过氧化氢测定⽅法.doc220花粉液体纯体外萌发⽅法.doc221花序浸染法转化拟南芥 - 副本.doc222基因枪法瞬时转化花粉.doc223甲基化⽔平重硫酸盐测序.doc224减数分裂染⾊体观察⽅法.doc225酵母感受态制备及转化.doc226磷酸盐缓冲液配制⽅法.doc227酶活测定⽅法.doc228免疫⾦⼿册.doc229茉莉酸JA提取.doc230拟南芥⾓质测定⽅法.doc231拟南芥解剖学图 - 副本.doc232拟南芥茎切⽚.doc233拟南芥原⽣质体转化系统.doc234凝胶迁移滞后实验 - 副本.doc235农杆菌感受态的制备.doc236农杆菌侵染拟南芥花序的转化⽅法 - 副本.doc237农杆菌转化番茄.doc238农杆菌转化烟草.doc239切⽚技术.doc240琼脂糖切⽚.doc241设计引物如何设计酶切位点.doc242⽯蜡包埋机(paraffin-embedding-station)Leica-EG1150H.doc 243⽯蜡切⽚制作⽇程(新).doc244实验室常⽤试剂、缓冲液的配制⽅法.doc245实验室⽣理⽣化指标实验简略步骤.doc246实验室原核表达步骤.doc247史上最全限制性内切酶酶切位点汇总.doc248树⽊DNA的提取.doc249双分⼦荧光互补实验原理 - 副本.doc250⽔稻根尖压⽚-2015-7-13.doc251⽔稻叶中CAT、POD和SOD活性的测定.doc252⽔稻营养液配⽅.doc253透明comfocs.doc254⼟壤速效钾测定.doc255⼟壤有机质测定.doc256⼟壤有效氮测定.doc257⼟壤有效磷测定.doc258⽡村单倍体诱导protocol.doc259我们实验室⽊本果树RNA提取⽅法.doc260烟草注射.doc261叶绿素含量测定.doc262液泡分离及V-PPase酶活和H 转运检测.doc263仪器操作规范-2017.11.17.doc264荧光定量引物⾃动化设计教程.doc265营养品质测定⽅法.doc266⽤于质谱鉴定的蛋⽩质凝胶样品酶解⽅法.doc267油菜转基因技术体系终结版.doc268原位.doc269中药鉴定学重点整理.doc270最新实验原理与⽅法2009.doc271[]进化树软件MEGA最新6.06说明书.docx2722个试验⽅法分享.docx2731003总结载体构建说明书.docx27420170106电击法农杆菌感受态的制备及其转化-曹⾼燚.docx275A simple and rapid method for the preparation of plant genomic DNA for PCR analysis..docx 276Benzoxazinoids extraction and quantification.docx277BSMV基因沉默相关.docx278ChIP for Plant.docx279circos画共线性图⽚.docx280CO-IP (2).docx281co-IP.docx282CoIP-Western blot.docx283CTAB法提DNA.docx284CTAB法提取⽔稻DNA.docx285Dellaporta法⼩量快速提取植物全基因组DNA.docx286EMSA protocol.docx287EST-SSR实验流程及软件使⽤.docx288FAA固定protocol.docx289FNPI-PCR⽅案.docx290GISH分析.docx291His-tag原核蛋⽩提取.docx292Histochemical detection of 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326改良的⽟⽶SLS法提取DNA.docx327柑橘DNA和RNA提取⽅法.docx328感受态细胞制备.docx329花青素原花⾊素相关protocol.docx330活性氧,胼胝质,叶⽚⽓孔的检测实验.docx331基础分⼦⽣物学实验操作指南.docx332基因枪轰击洋葱表⽪观察.docx333基因枪瞬时转化矮牵⽜花瓣.docx334简单粗暴提取拟南芥DNA-1.docx335酵母双杂筛库1.docx336酵母双杂实验⽅法总结.docx337酵母双杂最终版.docx338酵母转化⽅法及⽔稻组织培养液配⽅-1.docx339聚丙烯酰胺凝胶电泳检测PCR产物.docx340菌落PCR(⾃⼰总结).docx341可溶性糖测定.docx342临界点⼲燥.docx343拟南芥材料快速PCR鉴定.docx344拟南芥可溶性全蛋⽩的双向电泳.docx345拟南芥原⽣质体.docx346拟南芥原⽣质体的提取和转化.docx347拟南芥原⽣质体亚细胞定位Copy of Localization of procedure.docx348拟南芥原⽣质体制备.docx349拟南芥杂交.docx350拟南芥总蛋⽩简易提取.docx351凝胶迁移实验(EMSA)实验⽅法 - 副本.docx352农杆菌注射烟草顺时转化.docx353全基因组甲基化WGBS和转录组分析过程.docx354试验⽅法.docx355⽔稻缺素处理配⽅及步骤.docx356⽔稻原⽣质体分离及转化.docx357⽔稻原⽣质体提取及转化.docx358⽔稻原⽣质体制备和瞬时转化.docx359体外泛素连接酶活性测定.docx360⼟壤基本指标检测.docx361兔源抗体制备.docx362新Y1H载体构建及转化步骤.docx365亚细胞定位.docx366烟草瞬时表达.docx367叶⽚碳代谢相关物质和酶试验⽅法-棉花.docx368油菜下胚轴暗光培养转化.docx369原核表达.docx370原核表达protocol.docx371原核表达步骤.docx372原核蛋⽩的提取.docx373原⽣质体叶⾁细胞瞬时表达.docx374原位杂交.docx375真核细胞表达外源基因技术⽅法及操作步骤.docx376植物常见外援激素的作⽤.docx377植物⽣理⽣化指标.docx378植物⽯蜡切⽚.docx379植物有效磷测定.docx380植物总RNA提取与琼脂糖凝胶电泳分析.docx381注射烟草看BiFC.docx382SA测定⽅法3832007_Dolezel_NATURE_PROTOCOLS_2233.pdf384【代码M0101】常⽤溶液的配制.docx385AGROBACTERIUM COMPETENT CELLS.doc386Agrobacterium growth and transformation.doc387AutoDock&ADT教程(1).doc388cellulose content detection method.doc389CHIP protocal.docx390CRISPR-Cas9载体构建(⽔稻).docx391CTAB-96孔法⽔稻基因组DNA提取.doc392CTAB提取真菌DNA.docx393DAB染⾊,台盼蓝染⾊-钱礼超.docx394【代码M0201】PCR引物设计⽅法.docx395【代码M0202】PCR引物设计11条黄⾦原则.docx396ELISA的原理和技术要点.pdf397EMS mutagenesis.docx398Endnote简易教程-郑夏⽣.ppt399Floral dip transformation 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Protocal正确.doc435AFLP实验流程.doc436staining for pollen viability.pdf437Taqman设计总结005.doc438【代码M0305】质粒DNA的转化.docx439【代码M0306】菌落PCR.docx440⼆代测序信息分析.pdf441亚细胞定位.doc442免疫荧光004.doc443全氮、有机质测定⽅法.doc444农杆菌侵染烟草叶⽚表⽪细胞实验步骤.doc445农杆菌感受态制作.docx446分⼦克隆指南(上)().pdf447分⼦克隆指南(下)().pdf448分⼦植物育种-徐云碧-中⽂版.pdf449半薄切⽚流程.doc450基因枪瞬时转化洋葱表⽪.docx451实验室常⽤分⼦⽣物学实验技术.docx452实验室常⽤培养基的配制⽅法.pdf453AsA测定⽅法.doc454实验报告RRF纯化.xls455【代码M0307】细菌的转化与平板筛选.docx456【代码M0402】蛋⽩电泳.docx457快速提取酵母基因组DNA.docx458⼿动进样HPLC-RID使⽤⽅法002.docx459拟南芥成花⽹络2015.pdf460拟南芥转基因试验流程.pdf461提取DNA和制种聚丙烯酰胺凝胶.docx462【代码M0308】E.coli感受态细胞的制备(CaCl2法).docx463⽊质素测定⽅法.docx464根系测定.doc465【代码M0405】双向电泳.docx466植物Western Blot.docx467植物分⼦遗传研究室实验技术.docx468植物病毒 dot-ELISA 检测⽅法.docx469植物组织⽯蜡切⽚.docx470⽔果果实(淀粉糖含量⾼)RNA提取⾃⼰配药品⽅法(经济性).doc 471⽔稻茎结培养操作流程.doc472泛素化蛋⽩质组.docx473液相⾊谱操作指南003.docx474烟草瞬时转化.docx475⽟⽶原⽣质体转化及原核表达⽅案.docx476⽟⽶叶⽚原⽣质体拟制备转化操作流程.doc477cDNA⽂库构建.doc478电镜切⽚制样流程.doc479直径测量.pdf480短柄草农杆菌转化流程.doc481⽯蜡切⽚制作流程.docx482⽯蜡切⽚染⾊.docx483⽯蜡切⽚的制作.doc484⽯蜡包埋切⽚操作程序.doc485碱煮法快速提取⽟⽶基因组DNA.docx486种⼦发芽流程.docx487简易CTAB法提取植物基因组DNA.docx488组培苗操作流程.doc489【代码M0406】多克隆抗体的免疫电泳技术.docx490草莓⾼效稳定转化.docx491蛋⽩实验相关操作及注意事项.doc492蛋⽩质电泳实验技术-第2版 (1).pdf493DAB和NBT染⾊.doc494超级感受态细胞的制备.docx495转基因技术.ppt496转基因拟南芥.doc497酵母单杂.docx498酵母单杂交系统及其应⽤.doc499酵母双杂交张娟娟.doc500酵母双杂交⽂库筛选流程_mating法.docx501酵母双杂实验操作.WMV502酵母⽂库筛选-Mating篇.doc503酶法催化⽣产母乳脂肪替代物001.doc504酶活测定基本⽅法.doc505醇溶蛋⽩的提取与电泳.doc506限制性内切酶酶切位点汇总.docx507⾼通量测序原理,⽂库构建及质量评价-1108.pdf508鲍鱼酶裂解海带配⼦体细胞壁步骤-卞曙光-中国科学院海洋所.doc509麦⾕蛋⽩亚基的粗提与电泳.doc510(原创)傻⽠软件进⾏简并引物设计.ppt511【代码M0401】Western Blotting.docx51215. 琼脂糖切⽚.docx51316. 叶原基离体培养和活体成像.docx514AFLP原理和操作.docx515AI_及SS活性的测定.doc516DAPI staining of mature pollen nuclei.pdf517Design degenerate primer ——Important.docx518【代码M0407】单细胞凝胶电泳标准操作.docx519H2O2的测定-KI法.doc520His标签蛋⽩纯化试剂盒(可溶性蛋⽩).pdf521【代码M0403】蛋⽩电泳后的凝胶染⾊(考马斯亮蓝、银染、铜染).docx 522mRNA差异显⽰之变性聚丙烯酰胺凝胶电泳及银染.docx523NSC测定⽅法⽐较.docx524P32放射性同位素测定注意事 (2016修改版)(1).docx525Rubisco含量测定.docx526SS,SPS,R酶,Q酶,ADPGase酶活性.docx527【代码M0408】明胶酶谱分析法.docx528第七期⽣物数据分析技能实训会.doc529多靶点pCRISPR载体改良版(单⼦叶植物⽤)使⽤⽅法2014-10-31(P).docx 530发给⼤家后⼩艾⼜修改的---⽔稻转基因实验操作.doc531⽢薯花青素提取与测定⽅法2.doc532DAB染⾊.doc533国内重点实验室分⼦⽣物学实验⽅法汇总实验室常⽤实验⽅法.pdf534GSH 测定⽅法.doc535全国Python科研应⽤专题实操班.doc536实时荧光定量PCR技术.ppt537⽔稻多基因敲除系统.pdf538⽔果抗性相关指标的测定⽅法.docx539⼀种快速鉴定转基因植物纯合体的新⽅法.pdf540软件541【代码M0404】RNA电泳(甲醛变性电泳).docx542【代码M0409】对流免疫电泳.docx543【代码M0410】Southern Blotting实验步骤.docx544【代码M0411】Northern Blotting实验步骤.docx545【代码M0501】细胞膜蛋⽩的分离.docx546【代码M0503】蛋⽩质在原核⽣物中的表达.docx547植物RNA提取⽅法资料548液相测维⽣素E549ddRT-PCR(差⽰反转录PCR).docx550ec值.pdf551GLDH含量测定.pdf552Griess试剂测NO含量.doc553H2O2测定⽅法.doc554vigs侵染⽅法.doc555Western blot(聂).doc556苯丙氨酸解氨酶(PAL)活性的测定.doc557丙酮酸的测定.pdf558酚类和⽊质素.doc559过氧化氢测定⽅法(廖).doc560抗氧化酶和MDA以及蛋⽩测定指南.doc561类胡萝⼘素提取⽅法⽂档.doc562酶联免疫测ABA .doc563台盼蓝染⾊.doc564糖含量的测定1.pdf565糖含量的测定2.pdf566叶黄素提取.doc567叶绿素⽅法.doc568载体构建⽅法.doc569MDA含量测定.doc570POD_测定⽅法.doc571POD活性测定.doc572SOD活性测定.doc573sss测定.doc574标准曲线.xls575不同光质的H2O2和超氧(1).xls576根系活⼒测定⽅法.docx577脯氨酸标准曲线.xls578脯氨酸含量.doc579⽣理实验数据(SOD,POD,DPPH,超氧).xls580植物体内氧⾃由基含量测定.docx581植物体内游离脯氨酸含量的测定.doc582本⽣烟草种植和管理.docx583病毒接毒及其后续管理.docx584植物病毒dot-ELISA检测⽅法.doc585AKTA FPLC 蛋⽩纯化操作.doc586CTAB法提取RNA.doc587CTAB提取植物DNA.docx588DNA的琼脂糖凝胶电泳.doc589Greiss试剂测定NO的⽅法.doc590变性梯度凝胶电泳.doc591实验⽅法整理 .docx592线粒体测定最终版.docx593叶绿体提取.doc594【代码M0502】细菌总蛋⽩和膜蛋⽩提取⽅法.docx 595【代码M0504】免疫复合物的纯化.docx596【代码M0602】动物组织块DNA的提取.docx597半薄切⽚技术.ppt598⽯蜡切⽚流程.doc599透射电镜切⽚的步骤.doc600荧光参数.doc601植物RNA的提取及其电泳鉴定.doc6021-1 ⽯蜡切⽚的制作.pdf603N-P-K联合消煮测定.doc604SOD、POD、CAT、MDA和可溶性蛋⽩测定.doc 605沉降值测定⽅法.doc606FISH procedure-2.doc607PCR-SSCP实验操作详细步骤.doc608Promega公司的TA载体克隆与转化.doc609shotgun_⽂库的构建.doc610southern 杂交实验流程.doc611毕业⽣离室前注意事项.doc612基因定点突变.doc613基于⽑细管电泳的荧光检测SSR实验程序.doc614拟南芥培养转化的流程[1].doc615庞斌双的蛋⽩电泳和连接转化程序.doc616实时荧光定量PCR技术.doc617⼩麦成熟胚标准技术规程.doc618⼩麦基因组DNA的提取.doc619⼩麦⽥间记载和室内考种项⽬与标准.doc620怎样记录实验结果.doc621重点实验室protocol-CWF全长cDNA⽂库构建流程.doc622重点实验室protocol-总RNA提取(Trizol法)和mRNA提取[Oligo(dT)cellulose]流程zgy.doc 623ABI3730XL测序实验流程-2007-3-15.doc624BAC⽂库构建与筛选实验程序-2007-3-10.doc625PCR产物的TA克隆(DNA的胶回收和连接).docx626基因X 中⽂版.pdf627植物中基因编辑.docx628【代码M0604】CTAB法提取植物总DNA.docx629【代码M0605】磁珠法提纯mRNA.docx630【代码M0606】连续梯度法纯化闭环DNA.docx631【代码M0607】不连续梯度法纯化闭环DNA.docx632【代码M0608】从外周⾎淋巴细胞中提取RNA.docx633【代码M0609】饱和氯化钠法提取外周⾎DNA.docx634【代码M0701】荧光原位杂交(FISH)探针的制备.docx635【代码M0801】Conditional gene knockout in C. elegans- CRISPR-Cas9.docx636【代码M0802】Target Sequence Cloning Protocol- CRISPR-Cas9.docx637【代码M0803】SAM target sgRNA cloning protocol- CRISPR-Cas9.docx638【代码M0901】酵母双杂交筛选简介和流程.docx639【代码M0902】构建于 AD 载体的 cDNA ⽂库的扩增.docx640【代码M0903】构建于 AD 载体的 cDNA ⽂库的纯化.docx641【代码M0904】pGBKT7bait ⼩规模转化酵母菌 AH109.docx642【代码M0905】钓饵蛋⽩⾃⾝激活报道基因的活性检测.docx643【代码M0906】⽂库质粒⼤规模转化已携带钓饵质粒的酵母.docx644【代码M0907】转化⼦的筛选与库质粒纯化.docx645【代码M0908】酵母质粒 DNA 的提取.docx646【代码M0909】蛋⽩相互作⽤的验证—pGBKT7bait 与 pACT2 ⼩规模共转化酵母.docx 647【代码M1001】分⼦⽣物学实验常⽤培养基的配制⽅法.docx648【代码M0601】⼩量制备质粒DNA(强碱法).docx649【代码M0603】动物组织RNA提取及纯化.docx650Southern Blot原理及实验⽅法.doc651SSR分⼦标记实验操作及其注意事项.doc652单核苷酸多态性(SNP)实验.docx653动物基因组DNA的提取.doc654甲基化检测原理及步骤.doc655降落PCR.docx656聚合酶链式反应(PCR)基本操作步骤.doc657菌落PCR.doc658⽬的基因PCR扩增产物的克隆.doc659逆转录-聚合酶链反应(RT-PCR).doc660凝胶迁移实验(EMSA).docx661普通PCR、原位PCR、反向PCR和反转录PCR的基本原理和操作步骤.doc 662染⾊质免疫共沉淀(ChIP)实验.docx663实时荧光定量PCR具体实验步骤.doc664限制性⽚段长度多态性实验(RFLP).doc665原位PCR技术基本原理.doc666质粒DNA的提取.doc667重叠延伸PCR实验.docx668重组质粒的连接、转化及筛选.doc669(原创)实时定量神器Allele ID 5破解教程.doc670分⼦⽣物学⼤实验2-L.pptx671分⼦⽣物学实验II新教案.pdf672分⼦⽣物学实验673实验技术视频674现科课件675研究⽣课件676研究⽣分⼦⽣物学实验I讲义-2017.doc677⼤肠杆菌感受态细胞的制备和转化.doc678pGEMT.pdf679TaKaRa_LA_Taq说明书.pdf680TAKARA双酶切表.doc681分⼦克隆技术实验操作⼿册.pdf682宝⽣物去磷酸化说明书.pdf683构建载体知识查询.docx684载体图谱685载体图谱序列686上海硕盟_TOYOBO说明书.pdf687分⼦克隆实验指南第三版上册.pdf688分⼦克隆实验指南第三版下册.pdf6891 PCR实验技术6902 DNA实验技术6913 RNA实验技术6924 蛋⽩质实验技术6935 补充694DH5α感受态制备⽅法.pdf695EMSA696Easy Yeast plasmid isolation kit user mannual.pdf697GM配⽅.pdf698GST pull down.pdf699word700Immobilized Glutathione.pdf701MagneGST Pull-Down System.pdf702Ni-NTA handbook.pdf703PCR704PHARMACIA_GST_Gene_Fusion_System_Handbook.pdf705Pull-Down GST kit.pdf706RNAi707RNA提取⽅法——TRIZOL.pdf708SDS_PAGE胶配⽅表.pdf709Y2H Membrane Protein Kit.pdf710activation tagging.pdf711chip EZ-ChIP_manual.pdf712chromas.rar713his tag pull down assay .pdf714iTILLING.pdf715pfu.pdf716promega_GloMax2020完整版.pdf717western breeze chromo_man.pdf718western_blot技术集锦.rar719免疫共沉淀与Western Blot.pdf720原位杂交⽅法721国内重点实验室分⼦⽣物学实验⽅法汇总实验室常⽤实验⽅法.pdf 722常⽤缓冲液的配制.pdf723普通表达载体724植物细胞组织学⾼⽼师课件725荧光定量PCR.pdf726荧光激发及吸收波长.PDF727酵母双杂交728酵母双杂交技术研究与应⽤进展_李先昆.pdf729AxyPrep_DNA_Gel_DYE_Ext_Kit_ver20090727.pdf730His Spin Trap.pdf731In-Fusion Kit User manual.pdf732P020091009585923799380.ppt733PPICZalpha.pdf734[]基因克隆研究⽅法综述.pdf735matchmaker gold yeast one-hybrid library screening system.pdf736pET28_map.pdf737ppic9k_man.pdf738分⼦克隆实验指南上册v3.pdf739分⼦克隆实验指南下册v3.pdf740拟南芥T-DNA插⼊突变纯合体的鉴定.ppt741EMSA.pdf742淀粉检测说明书.pdf743RT-PCR744DNA kit.pdf745DP441 RNAprep pure多糖多酚植物总RNA提取试剂盒.pdf746Gateway_载体构建.ppt747RNAprep Pure 多糖多酚植物总RNA提取试剂盒--天根.pdf748系统发育分析课件2017完整版.pdf749Real-time PCR750office2013完美激活.rar751[3]实时荧光定量PCR技术_天根.ppt752[4]国内重点实验室分⼦⽣物学实验⽅法汇总实验室常⽤实验⽅法.PDF 753[7]PCR技术讲座.pdf754[8]国内重点实验室分⼦⽣物学实验⽅法汇总实验室常⽤实验⽅法(1).pdf755[11]荧光定量PCR技术讲座:理论基础、引物及探针设计、体系优化、实验⽅案、数据分析、污染防控 2.ppt756R语⾔绘图-实操训练.pptx757R语⾔绘图⼊门.pptx758基因功能分析.pptx759基因聚类分析和样品相关性分析.pptx 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setup.exe175Photoshop_CS3.exe176R-3.4.1-win.exe177RStudio-1.0.143.exe178SETUNA.exe179iSlideTools.Setup.Online.2.4.1.exe180HemI_windows_1_0_win32_x64.exe181HemI_windows_1_0_win32_x64 (2).exe182ApE.exe183MeV_4_9_0_r2731_win(1).rar184GraphPad Prism 7 教程及软件185信号通路作图软件186winmd5free---检测数据完整性.zip187全基因组扫描-转录因⼦结合位点-⼯具.pdf188⽤origin软件绘制折线图和误差线(By 蜗⽜菌).pdf 189多序列⽐对软件clustalx-2.1-win.msi190dnasp51001.msi191Sigmaplot.rar192SPSS22.0.rar。
分子进化树构建及数据分析的简介mediocrebeing, rodger, lylover , klaus, oldfish, yzwpf一、引言开始动笔写这篇短文之前,我问自己,为什么要写这样的文章?写这样的文章有实际的意义吗?我希望能够解决什么样的问题?带着这样的疑惑,我随手在丁香园(DXY)上以关键字“进化分析求助”进行了搜索,居然有289篇相关的帖子(2006年9月12日)。
而以关键字“进化分析”和“进化”为关键字搜索,分别找到2,733和7,724篇相关的帖子。
考虑到有些帖子的内容与分子进化无关,这里我保守的估计,大约有3,000~4,000篇帖子的内容,是关于分子进化的。
粗略地归纳一下,我大致将提出的问题分为下述的几类:1.涉及基本概念。
例如,“分子进化与生物进化是不是一个概念”,“关于微卫星进化模型有没有什么新的进展”以及“关于Kruglyak的模型有没有改进的出现”,等等。
2.关于构建进化树的方法的选择。
例如,“用boostrap NJ得到XX图,请问该怎样理解?能否应用于文章?用boostrap test中的ME法得到的是XXX树,请问与上个树比,哪个更好”,等等。
3.关于软件的选择。
例如,“想做一个进化树,不知道什么软件能更好的使用且可以说明问题,并且有没有说明如何做”,“拿到了16sr RNA数据,打算做一个系统进化树分析,可是原来没有做过这方面的工作啊,都要什么软件”,“请问各位高手用clustalx做出来的进化树与phylip做的有什么区别”,“请问有做过进化树分析的朋友,能不能提供一下,做树的时候参数的设置,以及代表的意思。
还有各个分支等数值的意思,说明的问题等”,等等。
4.蛋白家族的分类问题。
例如,“搜集所有的关于一个特定domain的序列,共141条,做的进化树不知具体怎么分析”,等等。
5.新基因功能的推断。
例如,“根据一个新基因A氨基酸序列构建的系统发生树,这个进化树能否说明这个新基因A和B同源,属于同一基因家族”,等等。
super six Owner’s Manual Supplement120858.PDFsafety information .................................. about this supplement .. (2)important Composites message (3)intended Use (3)Building Up a frameset (4)Bike stands (4)extreme temperatures (4)inspection and Crash Damage (5)repainting and refinishing (5)serial nUmBer (6)HeaDset (7)seat Post/ BinDer (8)si Bottom BraCKet sHell (9)Crankset Compatability (9)Bearing maintenance (9)BB30 to 68mm adapter (10)Cannondale tools (11)CHainstay ProteCtion (12)DerailleUr HanGer (13)Geometry/sPeCifiCation (14)rePlaCement Parts (15)Please note that the specifications and information in this manual are subject to change for product improvement. for the latest product information, go to http://www.cannondale. com/tech/.CONTENTSSAFETY INFORMATIONAbout This Supplement Cannondale owner’s manual supplements provide important model specific safety, maintenance, and technical information. t hey are not replacements for your Cannondale Bicycle Owner’s Manual.this supplement may be one of several for your bike. Be sure to obtain and read all of them.if you need a manual or supplement, or have a question about your bike, please contact your Cannondale Dealer immediately, or call us at one of the telephone numbers listed on the back cover of this manual.you can download adobe acrobat PDf versions of any Cannondale owner’s manuals or supplements from our website: http://www. /bikes/tech.• This manual is not a comprehensive safety or service manual for your bike. • This manual does not include assembly instructions for your bike.• All Cannondale bikes must be completely assembled and inspected for proper operation by a CannondaleDealer before delivery to the owner.2120858.PDF3Intended Usethe intended use of your bike or frameset isConDition 1 / HiGH PerformanCe roaD.ConDition 1 / HiGH-PerformanCe roaD bikes and framesets are designed for riding on a paved surface where the tires do not lose ground contact. they are not intended to be ridden off-road, cyclocross, or touring with racks or panniers.material use is optimized to deliver both light weight and specific performance. you must understand that (1) these types of bikes are intended to give an aggressive racer or competitive cyclist a performance advantage over a relatively short product life, (2) a less aggressive rider will enjoy longer frame life, (3) you are choosing light weight (shorter frame life) over more frame weight and a longer frame life, (4) you are choosing light weight over more dent resistant or rugged frames that weigh more. all frames that are very light need frequent inspection for cracks that would indicate that the frame is worn out from fatigue. these frames are likely to be damaged or broken in a crash. they are not designed totake abuse or be a rugged workhorse.Maximum Weight Limit* Seat Bag /Handlebar Bag OnlyBuilding Up A Frameset Before building up a frameset, consult with your Cannondale Dealer and the component manufacturers, and discuss your riding style, ability, weight, and interest in and patience for maintenance.make sure the components chosen are compatible with your bike and intended for your weight and riding style.Generally s peaking, l ighter w eight c omponents have shorter lives. in selecting lightweight components, you are making a trade-off, favoring the higher performance that comes with less weight over longevity. if you choose more lightweight components, you must inspect them more frequently. if you are a heavier rider or have a rough, abusive or “go for it” riding style, buy heavy duty components. read and follow the component manufacturers warnings and instructions.Bike Standsthe clamping jaws of an ordinary bike stand can generate a crushing force strong enough to seriously damage and ruin your bike frame.Protect FromExtreme Temperatures• Protect your carbon bike from extreme temperatures when storing or transporting it. • allow your bike to cool off or warm up before you ride• Do not store your bike in places where the temperature will exceed 66.5C° (150°f). for example, do not leave your bike lying flat in a black pickup truck bed in the desert sun or under the glass of a hatchback auto.4120858.PDF5Inspection & Crash Damageof Carbon FramesRepainting Or RefinishingYou should not paint over the existing finish, refinish or repaint your bike. The carbon fiber composites making up the frame are held together by some extremely strong bonding chemicals. However, these bonds can be attacked or weakened bypaint stripping or refinishing chemicals.figure 2.Serial Numberthe serial number (1) printed and permanently affixed barcode label.Use this serial number for warranty registration and theft recovery. see your Cannondale Bicycle Owner’s Manual for more information on warranty registration.Bottom Bracket Guidethe cable guide (2) is mounted on the bottom bracket. replacements are available through a Cannondale Dealer. the kit # is Kf363/VentKeep the small vent hole (3) in the bottom of the bottom bracket shell clear of any dirt so that any water can drain out.Water can enter into frame tubes through washing, condensation, wet rides, or rain. it penetrates or can be sprayed in through water bottle bosses, seat tube, head tube and seat post openings. Water will not chemically harm your bike. if it freezes, the ice expansion can burst or crack the frame making it unsafe to ride. this damage is not covered under your warranty.After wet rides, remove seat post, invert bike to drain any water.6120858.PDF7headset48m mKP017/QC778/35mmQC777/KP044/figure 3a.Use only original Cannondale parts. see Carbon Road Fork Owner’s ManualSupplement 120860.pdf on our website /tech_center/SAZ 111594The addition of shim(s) between the compression ring and top caps may be needed for top cap to head tube clearance.KP044/ - KIT,HEADSET,SUPERSIXNO STAR NUTSThe addition of shim(s) between the compression ring and top caps may be needed for top cap to headtube clearance.48 mmSI Compression Assembly KP017/ InstructionsThe following procedure should only be completed by a professional bike mechanic.1. assemble the fork, headset, spacers, and stem without tightening the stem bolts onto thehead tube. When the system is assembled, the carbon steerer tube should be 2-3 mm below the top stem. all spacers must be located below the stem and within the maximum stack height as shown. no spacers may be used above the stem.2. set-up the compression assembly before inserting it. adjust the length so that the expanderis located at lower stem bolt. the top cap and the expander end provides critical support to the carbon steerer when tightening the stem bolts. adjust the length by threading the top cap on the expander parts.3. When the assembly is the correct length, insert it into the steerer tube. it is designed to fitsnugly inside the steerer. insert a 5 mm allen key through the access hole in the toP CaP and into the eXPanDer Bolt. tighten the expanding parts by turning clockwise to 6.8nm, 5 ftlbs.4. now, to set bearing preload, insert a 6mm allen key into the hex shape in the toP CaPitself. turn the entire top cap clockwise to increase preload. turning it counter-clockwise will decrease the preload. When the headset preload feels correct, turn the stem to align the handlebar and tighten the stem fork clamp bolts to the torque specified for the stem.Consult the stem manufacturer’s instructions. the torque values for components are often marked on the part.8120858.PDF9seat post / binderfigure 4.Installation1. make sure the seat post, binder, and seat tube are clean. Use a dry towel.2. apply small amount of seat post gel (Cannondale kit Kf115/) to seat post surface and reinstall the post into the seat tube. 4 FOR HIGH-PERFORMANCE CARBON SEAT POSTS ALWAYS POSITION THE SEAT BINDER SLOT OPPOSITE THE SEAT TUBE SLOT. locating the binder slot opposite the seat tube slot reduces the chances of cracking from deformation or accidental overtightening.tighten the seat binder with a torque wrench. DO NOT EXCEED MAXIMUM SPECIFIED TORQUE 6.8Nm.5. Check tightening torques of the saddle to seat post clamp bolts. too much torque may fail the bolts. too little torque will result in movement which leads to fatigue and bolt failure.WARNINGTO AVOID SEAT POST OR FRAME DAMAGE:KF362/BBQKF115/SEAT TUBE SLOTBINDER SLOT10bottom bracket shellfigure 5.Crankset Compatabilitythe BB shell is compatable with the BB30 standard. see / for information see SI Cranksets Owner’s Manual Supplement . see /tech/.Bearing Maintenanceshell bearings (KB6180/) are sealed cartridge type and do not require lubrication. inspect bearing condition annually (at a minimum) and anytime the crankset assembly is disassembled or serviced. the bearings are a press fit within the shell. old bearings should not be reinstalled if removed. replace both bearings at the same time.replacements circlips (QC616/) are available if the circlips become damaged. the circlips can be lifted from the BB groove (inset) by lifting the hooked end with a thin blade screwdriver.KB6180/QC616/120858.PDF BB30-to-68mm Standard Adapterthe adapter (Cannondale kit Kf365/) converts the BB30 bottom bracket cranksets for use with 68mm bottom brackets. once installed, the adpater can not be removed.the adapter is not a repair part and will only work in undamaged frames in good condition. improper installation or removal can result in damage and void applicable frame warranty.Cannondale ToolsKt011/ is a bearing removal tool. Kt010/ is a set of bearing installation tools to be used with a standard headset press. Kt013/ a two piece tool set required for removing the crankarms si Hollowgram alloy cranksets. for information see SI Cranksets Owner’s Manual Supplement. see /tech/.120858.PDF chainsta y protectionthe chainstay plate (Cannondale kit KP045/) located on the right chainstay just behind the chainrings, protects the chainstay from damage in the event the chain is dropped from the chainring. Contact your Cannondale Dealer for a replacement if it is becomes missing or damaged.KP045/figure 8.the clear chainstay protector provides limited protection against frame or finish damage causedby the chain. replacement protectors are available through a Cannondale Dealer. Array figure 9.Check derailleur adjustment after replacement. readjust wheel quick release so it is very tight.see Part i section 4. a in your Cannondale Bicycle Owner’s Manual.120858.PDFreplacement partsfor an up to date list of kits available for your bike, please visit our tech Center at : http://www.cannondale.com/bikes/tech/。
mega macs66Quick Start Guide en QSMM66V5200EN0917S0Table of Contents mega macs66 Table of Contents1About this Quick Start Guide (4)1.1Reading the Manual (4)2Safety Precautions (5)2.1Safety Precautions–Risk of Injury (5)2.2Safety Precautions for the mega macs66 (6)3Product Description (7)3.1Delivery Contents (7)3.2Intended use (8)3.3Using the Bluetooth Function (9)3.4Scope of Functions (9)3.5Display (9)3.6Connections of the mega macs66 (10)4Installation of the Hella Gutmann Drivers Package (13)4.1System Requirements of Hella Gutmann Drivers (13)4.2Installation of the Hella Gutmann Drivers Package (13)5Putting Into Operation (14)5.1Charging the Battery (14)5.2Switching on the Device (14)5.3License Release (15)5.4Switching Off the Device (15)6Configuring the Device (17)6.1Configuring Interfaces (17)mega macs66Table of Contents7Working with the Device (21)7.1Icons (21)7.2Diagnostics (25)7.3Calling Up Vehicle Information (28)7.4Oscilloscope (28)About this Quick Start Guide mega macs66 Reading the Manual1About this Quick Start GuideThe quick start guide comprises the most important information in aclearly visible form to facilitate the start with the mega macs66.1.1Reading the ManualYou can find the manual with detailed information about the use of yourmega macs66in the device under or on the homepage of HellaGutmann /de/workshop-solutions/diagnose.This page provides the current download versions of the manuals andquick start guides as well as other valuable product leaflets for optionalaccessories and products of Hella Gutmann,which can support you in yourdaily work.mega macs66Safety PrecautionsSafety Precautions–Risk of Injury 2Safety Precautions2.1Safety Precautions–Risk of InjurySafety Precautions mega macs66 Safety Precautions for the mega macs662.2Safety Precautions for the mega macs66mega macs66Product DescriptionDelivery Contents 3Product Description3.1Delivery Contents3.1.1Checking delivery contentsPlease check the delivery contents upon receiving your device so thatcomplaints can be issued immediately regarding any potential damage.Product Description mega macs66 Intended useProceed as follows to check the delivery contents:1.Open the package supplied and check for completeness based on thedelivery slip.Should you identify any damage to the package,then open the packagein the presence of the delivery service and check the device for hiddendamage.Any transport damage to the package supplied and damageto the device shall be registered in a damage report by the deliveryservice.2.Take the device out of the packaging.3.Check the device for mechanical damage and shake it slightly toensure that there are no loose parts inside.3.2Intended useThe mega macs66is a mobile device for detecting and rectifying faults inautomotive electronic systems.The device enables access to extensive technical data such as wiringdiagrams and service data,set values and descriptions of vehicle systems.A lot of this data is transferred to the device directly from the HellaGutmann diagnostic database via online connection.Therefore,the devicemust be permanently online.The device is not suitable for repairing electrical machines and tools orhome electrics.Diagnostic devices from other manufacturers will not besupported.If the device is used in a way not authorized by Hella Gutmann,the safetyof the device may be influenced.mega macs66Product DescriptionUsing the Bluetooth Function The device is intended for industrial use.Outside of industrialenvironments,e.g.in commercial areas or in the center of a town,radiointerference suppression measures may be necessary.3.3Using the Bluetooth FunctionThe terms of use of the Bluetooth function may be restricted or prohibitedthrough law or corresponding legal regulations in certain countries.Pay attention to the provisions in force in the respective country beforeusing the Bluetooth function.3.4Scope of FunctionsThe range of functions of the mega macs66depends on the country,thelicenses acquired,and/or the optionally available hardware.Thisdocumentation may therefore describe functions that are not available onthe individual device.Missing functions can be enabled by acquiring acorresponding license subject to charge and/or additional hardware. 3.5DisplayProduct Description mega macs66 Connections of the mega macs663.5.1Operating the DeviceThe device is equipped with a touch screen display.All menus andfunctions can be selected and/or activated by slightly touching with thestylus or the finger or just by pressing the arrow keys.3.6Connections of the mega macs66mega macs66Product DescriptionConnections of the mega macs66Product Description mega macs66 Connections of the mega macs66mega macs66Installation of the Hella Gutmann Drivers PackageSystem Requirements of Hella Gutmann Drivers 4Installation of the Hella Gutmann Drivers Package 4.1System Requirements of Hella Gutmann Drivers•Microsoft Windows7or higher•Windows administrator rights4.2Installation of the Hella Gutmann Drivers PackageTo obtain all the data about the related vehicle provided by Hella Gutmann,the device requires a permanent online connection and the installed driverpackage Hella Gutmann Drivers.To keep the connection costs down,HellaGutmann recommends a DSL connection and a flat rate.1.Install the Hella Gutmann Drivers on the office or repair shop PC.The driver package Hella Gutmann Drivers program is on the suppliedHGS data carrier.2.Connect the device to a web-compatible PC.Once the connection symbol in the top symbol bar changes fromblack to green,the online connection has been set up successfully andis active.Putting Into Operation mega macs66 Charging the Battery5Putting Into OperationThis section gives a description of how to switch on and off the device aswell as all the necessary steps for the first use of the device.5.1Charging the BatteryPrior to putting the device into operation,charge the battery for at least8to10h(device is switched off meanwhile).Proceed as follows to charge the battery:1.Press in main switch until it locks into place.The electric circuit to the battery is now closed.2.Insert the voltage supply plug into the device's socket.3.Insert the power plug into the plug socket.The battery is charged.5.2Switching on the DeviceTo switch on the device:1.Press in main switch until it locks into place.The device switches to standby mode.2.Lightly touch the display.The general terms and conditions appear.3.Read the general terms and conditions and confirm them at the end ofthe text.A user selection window appears.The respective user name is saved for all data stored in the CarHistory.This enables quicker identification of the mechanic whoperformed the repair work if a query is subsequently made.4.Double-click.mega macs66Putting Into OperationLicense Release5.Enter the user name.6.Confirm your entry with.7.Activate the Stay logged in check box if necessary.If the Stay logged in check box is activated,you will not need to selecta user name when switching on in the future.8.Confirm your entry with.The input is saved automatically.The main menu appears.Now you can start working with the device.5.3License ReleaseProceed as follows to connect the device with the HGS server:1.Select Contracts under>Settings in the main menu.2.Select the>License<tab.3.Retrieve My licences with.Data is downloaded.Purchased licences are displayed.4.Switch the device off and on again.Now you can start working with the device.5.4Switching Off the Device.Proceed as follows to switch off the device:1.Switch off the device with.2.Observe the confirmation prompt.Putting Into Operation mega macs66 Switching Off the Device3.Switch off the device with.Abort the procedure with.After switching off,the device is in standby mode.mega macs66Configuring the DeviceConfiguring Interfaces6Configuring the DeviceConfigure all interfaces and functions under>Settings<in the main menu.6.1Configuring InterfacesSet the interfaces for the printer,BPC-Tool,Ethernet,Bluetooth,WLANand UMTS module here.Configure all interfaces of the device under Settings>Interfaces.If there are several possible connections to devices or tools,the fastestand most stable connection is always preferred.The hierarchy for connection is as follows:1.EthernetB3.Bluetooth4.WLAN6.1.1Configuring the EthernetYour network settings can be made here.Proceed as follows to connect the device to a network(router)via anEthernet interface:1.Insert the Ethernet cable(not included in the delivery contents)into theEthernet port of the device and the remote station.2.Select Interfaces under>Settings in the main menu.3.Select the>Ethernet<tab.4.Open a list under IP address mode with.If>Determine automatically(DHCP)<is set,then the deviceautomatically searches for the IP address.If>Determine manually<is selected,you must enter the address ofthe remote station under mega macs IP address,e.g.192.168.255.255.5.Select>Determine automatically(DHCP)<or>Determinemanually<.The selection will be saved automatically.Configuring the Device mega macs66 Configuring Interfaces6.1.2Configuring the Bluetooth AdapterHere you can configure the Bluetooth adapter.The integrated Bluetooth module enables a wireless connection to a PC onwhich the driver package Hella Gutmann Drivers is installed.Searching for the Bluetooth AdapterProceed as follows to search for the Bluetooth adapter:1.Insert the Bluetooth adapter into the USB port on the PC.2.Select Interfaces under>Settings in the main menu.3.Select the>Bluetooth<tab.4.Activate the check box to make settings.A confirmation prompt appears if WLAN was previously activated inthe device.5.Observe the confirmation prompt.6.Confirm the confirmation prompt with.7.Do this via Search for Bluetooth adapter.8.Observe the information window.9.Confirm the information window with.Connection is established and the search for a Bluetooth adapter is inprogress.Once the connection from the device to the Bluetooth adapter has beenset up successfully,a selection list of the Bluetooth adapters that havebeen found is then displayed.mega macs66Configuring the DeviceConfiguring Interfaces10.Select the desired Bluetooth adapter.The selection will be saved automatically.The automatically assigned Bluetooth adapter address appears in the fieldBluetooth adapter address.6.1.3Searching for and installing a WLAN interfaceProceed as follows to connect the device to a network(router)throughWLAN interface:1.Select Interfaces under>Settings in the main menu.2.Select the>WLAN<tab.3.Activate the check box to make settings.A confirmation prompt appears if either Bluetooth or UMTS waspreviously enabled on the device.4.Observe the confirmation prompt.5.Confirm the confirmation prompt with.6.Open a list under IP address mode with.A drop-down list appears.If>Determine automatically(DHCP)<is set,the device automaticallysearches for the IP address.This option is set ex works.If>Determine manually<is selected,you must enter the address ofthe remote station under mega macs IP address,e.g."192.168.255.255".7.Select>Determine automatically(DHCP)<or>Determinemanually<.The selection will be saved automatically.8.Create wireless network with.The device searches for wireless networks.Once the device has successfully finished searching for wirelessnetworks,a pick list of wireless networks found is displayed.9.Select the required wireless network.10.Regard the window with information and instructions.11.Confirm the info and instructions window with.12.Enter the WLAN password.Configuring the Device mega macs66 Configuring Interfaces13.Confirm the password with.The input is saved automatically.The following message appears if the wireless network has been setup successfully:•the name of the selected wireless network under Wirelessnetwork(SSID)•the security system of the selected wireless network under WLAN security•the IP address of the installed Hella Gutmann Driver underGutmann Portal IP address14.Click the symbol on the right of the header to check the connectionstatus.The device is connected to the Internet if Data server is displayed underConnection and connected under WLAN.You can use the WLAN connection now.mega macs66Working with the DeviceIcons 7Working with the Device7.1Icons7.1.1General SymbolsWorking with the Device mega macs66 Icons7.1.2Symbols in the Main Menumega macs66Working with the DeviceIconsWorking with the Device mega macs66 Icons7.1.3Symbols in the Vehicle Selection Menumega macs66Working with the DeviceDiagnostics7.2Diagnostics7.2.1Preparing Vehicle DiagnosticsThe selection of the correct vehicle is a basic precondition for trouble-freevehicle diagnostics.The device provides assistance to simplify theselection,e.g.indicating the installation position of the diagnostic port orvehicle identification through VIN.The following ECU functions are possible under>Diagnostics<in the mainmenu:•Trouble code reading•Parameter readout•Actuator test•Service reset•Basic setting•Coding•Test functionProceed as follows to prepare vehicle diagnostics:1.Select the desired vehicle under>Vehicle selection<in the mainmenu.2.Select>Diagnostics<in the main menu.Working with the Device mega macs66 Diagnostics3.Take the diagnostic module(DT66)out of the mega macs66.4.Insert the ST2connector into the ST2socket of the DT66.mega macs66Working with the DeviceDiagnostics5.Insert the OBD plug into the vehicle's diagnostic connector and placethe DT66inside the vehicle cabin.6.Select>Diagnostics<in the main menu.Now you can select the type of diagnostics.7.2.2Performing DiagnosticsProceed as follows to perform diagnostics:1.Perform steps1to5as described in section Preparing vehiclediagnostics(Page25).e to select the desired diagnostics under Function,Assemblyand System.3.Observe the information window,note window and instruction windowas applicable.4.Start the communication with.The diagnostic procedure between the device and the DT66takesplace via Bluetooth.The connection to the DT66is established if thesymbol changes from black to green.Working with the Device mega macs66 Calling Up Vehicle Information5.Repair the vehicle.Then delete the saved trouble codes from thevehicle system.7.3Calling Up Vehicle InformationHere you have an overview of the vehicle information including thefollowing:•Car History•Component Help•Service dataProceed as follows to call up vehicle information:1.Select>Vehicle information<in the main menu.2.Select the desired type of information using the symbols.Certain types of information may not be available depending on theselected vehicle.7.4OscilloscopeThe use of measuring technology requires the optionally availablemeasurement module(MT66).The oscilloscope can be used for measuring and/or depicting the followingmeasured variables:•Voltage•Current•Resistance•Temperature•PressureCurrent measurements are allowed exclusively using the clamp meterfrom Hella Gutmann.Depending on the required measurement,differentmeters are to be used.mega macs66Working with the DeviceOscilloscopeA light blue bar in the top toolbar indicates how much of the memoryspace reserved for this purpose in the Car History has been used.If theblue bar is complete,the oldest data is deleted from the Car Historymemory and the free memory is assigned new data.7.4.1Performing oscilloscope measurementsProceed as follows to perform oscilloscope measurements:1.Select>Measurements<in the main menu.2.Select the>Oscilloscope<tab.The window for measured variables and channels appears.3.Plug in the test lead and/or signal cable into the MT66.4.Connect the test lead to the component in question if necessary.5.Where necessary,connect the signal cable to the infrared thermometerof Hella Gutmann or the LPD kit.Working with the Device mega macs66 Oscilloscope6.Should you use the green current clamp(CP40),black current clamp(CP200),or blue current clamp(CP700),then connect it with thearrow pointing towards the battery around all positive cables,or withthe arrow pointing away from the battery around all negative cablesrespectively.7.Activate the check box for the desired measured variable and theoscilloscope channel.8.Confirm the selection with.Measurement will be started.e to set the time and measured variable ranges.The ideal measuring range of the device can alternatively bedetermined automatically with.10.Start Auto Set with>.mega macs66Notes31HELLA GUTMANN SOLUTIONS GMBHAm Krebsbach279241IhringenGERMANYPhone:+4976689900–0Fax:+4976689900–3999**********************©2017HELLA GUTMANN SOLUTIONS GMBH1STUECK/PIECE(S)���������������9XQ460986-341Made in Germany。
美格— 62使用说明书感谢选用美格62产品。
MEGA62是本公司继MEGA64之后又一鼎力之作。
为当今供水行业顶级专家厚积薄发,博采众家之长,精心打造的恒压供水行业的航空母舰。
该控制器性能卓越,功能强大,为简单应用用户提供更加稳定可靠的性能;为复杂尖端应用用户提供了更加灵活自由的供水设计解决方案。
62沿用了64蓝底白字四行八位的液晶屏全中文显示模式,并可直接挂接彩色触摸屏,操作更加直观简单方便。
该机器具有如下功能特点:1、多泵组设计,六台大泵加两台附属小泵,大泵、小泵皆可变频工频任意状态设定。
所有泵皆可实现故障互投或作处理对策。
2、分体结构,主机与显示器或触摸屏、上位机可分离。
专家自整定+模糊控制+现场优化经验处理,更符合供水控制特点。
3、界面美观,操作直观,全中文显示,分级菜单设定,无底层代码,无说明书也能自如设定应用;高低级功能设置,特殊场合亦可满足复杂要求。
4、控制模式、停车方式、大小分时、实时时间、目标值、当前值、频率、泵状态、各种报警均可同步显示;换泵倒计时、巡检倒计时、公司信息、电话、消防、休眠等等一目了然。
5、控制安全,各种工况处理对策可选。
6、双恒压可选,可通过开关量设定稳压值,或者决定消防功能启用与关闭。
7、多达八时段压力控制,且每时段内均可进行任意的压力设定控制及实现八时段的定时开关机功能。
8、智能节能休眠模式,解决行业内高层建筑休眠难题;主泵、小泵皆可休眠,小泵变量亦可休眠;压力提升休眠模式,并可控制小泵进休眠状态时间。
9、大小泵优先选项,并可锁定、禁止小泵运行时段,智能的定时换泵及独特的夜间换泵功能,正负反馈方式。
10、无负压功能,支持电接点或第二模拟量输入模式,且二者皆可自由设定;消防及巡检功能,消防泵巡检间隔及时长可随意设定,符合公安部最新消防标准。
11、延时启动功能,软停车功能,解决高层水锤及保护管网,获得平稳控制效果。
12、自动抑制表头抖动及系统振荡,让系统更稳定,模拟输出增益更宽可调。
基于线粒体COI基因序列的壮真蝎与普洱真蝎的分子鉴定李蓉;郑雨田;李春青;陈艳艳;杨振升;陈善元;肖蘅【摘要】对蝎类物种的传统分类主要依靠形态和行为特征,但由于该类群种间形态特征极为相似,物种的划分和鉴定困难.为弥补传统分类方法的不足,本研究以线粒体细胞色素氧化酶亚基Ⅰ (COI)基因作为分子标记,对形态相似的壮真蝎Euscorpiops validus和普洱真蝎E.puerensis进行分子水平的物种鉴定.采用PCR 扩增测序获得壮真蝎与普洱真蝎共24个样本的COI基因部分片段序列(660 bp),进行了遗传距离、系统发育及单倍型网络图分析.结果显示:壮真蝎15个样本中共检测到4个单倍型,单倍型之间的相似度为99.3%~99.8%;普洱真蝎9个样本中共检测到4个单倍型,单倍型之间的相似度为99.6%~99.8%;2种蝎的种间序列相似度为90.1%~90.6%,单倍型间的稳定差异核苷酸位点数为61个.壮真蝎与普洱真蝎种内平均遗传距离分别为0.0040、0.002 3,种间平均遗传距离为0.103 9,且种间遗传距离为种内的34.6倍.此外,分子系统发育树显示壮真蝎与普洱真蝎的单倍型序列各自聚为2个单系枝,且具有很高的分枝自举值(100%).单倍型网络图结果也显示壮真蝎与普洱真蝎8个单倍型明显分为2大类群,且壮真蝎的单倍型HAP2与普洱真蝎的单倍型HAP7之间的突变步数高达62步.上述结果不仅进一步确认壮真蝎与普洱真蝎为2个不同的物种,且表明线粒体COI基因可用于开展真蝎属Euscorpiops物种的分子鉴定.%The traditional classification and taxonomy of scorpiones are mainly based on morphological and behavioral characteristics.However,due to the similar morphological characteristics among species,it is difficult to classify and identify distinct species among scorpions.To compensate for the insufficiency of traditional taxonomic methodology,this study used mitochondrial cytochrome oxidase subunit I(COI) gene as molecular marker to conduct molecular identification of 2 morphologically similar scorpion species Euscorpiops validus andE.puerensis.The partial COI gene sequences (660 bp) from 24 samples of E.validus and E.puerensis were amplified by PCR followed by gene sequencing.The genetic distances,phylogenetic and haplotype network analyses were then carried out.The results showed that:4 haplotypes with similarity of 99.3%-99.8% were detected in 15 individuals of E.validus,and4 haplotypes with similarity of 99.6%-99.8% were found in 9 individuals ofE.puerensis;the interspecific similarity was 90.1%-90.6% and the number of stable differentiated nucleotide sites between the haplotypes of 2 species was 61.The intraspecific average genetic distances among E.validus haplotypes and among E.puerensis haplotypes were 0.004 0 and 0.0023,respectively,while the interspecific average genetic distance between E.validus and E.puerensis was O.103 9,being 34.6 times higher than that of intraspecific values.In addition,molecular phylogenetic tree clearly showed that the haplotype sequences of E.validus and E.puerensis clustered as 2 reciprocally monophyletic clades with high bootstrap values (100%).The haplotype network also showed that 8 haplotypes of E.validus andE.puerensis can be clearly divided into 2 clades,and the mutation steps between HAP2 haplotype of E.validus and HAP7 haplotype of E.puerensis reached 62.These results further confirmed that E.validus and E.puerensis were 2 distinct species,and indicated that mitochondrial COI gene was suitable for molecular species identification of Euscorpiops species.【期刊名称】《四川动物》【年(卷),期】2017(036)002【总页数】6页(P139-144)【关键词】壮真蝎;普洱真蝎;COI基因;分子鉴定【作者】李蓉;郑雨田;李春青;陈艳艳;杨振升;陈善元;肖蘅【作者单位】云南大学生命科学学院,昆明650091;云南大学生命科学学院,昆明650091;云南大学生命科学学院,昆明650091;云南大学生命科学学院,昆明650091;云南大学生命科学学院,昆明650091;云南大学生命科学学院,昆明650091;云南大学生命科学学院,昆明650091【正文语种】中文【中图分类】Q953;Q38壮真蝎Euscorpiops validus和普洱真蝎E. puerensis均属节肢动物门Arthropoda螯肢动物亚门Chelicerata蛛形纲Arachnida蝎目Scorpiones真蝎科Euscorpiidae真蝎属Euscorpiops,为真蝎属中等大小的物种(Kovaík et al.,2015)。
Vol.33,No.6Dec. 2020第33卷第6期2020年12月水产学杂志CHINESE JOURNAL OF FISHERIES文章编号:1005-3832( 2020 )06-0028-06用DNA 条形码鉴定常见鱼翅的种类与真伪熊娟,黄启红,陈敏儿,方军(广东省科学院,广东省测试分析研究所(中国广州分析测试中心),广东省化学危害应急检测技术重点实验室,广东省保健食品功效成分检测与风险物质快速筛查工程技术研究中心,广东广州510070)摘要:用形态鉴定了物种的市面上常见的6种正品鱼翅作标准样品,以明胶为主要原料制作的2种假鱼翅作阴 性对照,用DNA 条形码技术研究了广东省市场流通的35种鱼翅的遗传多样性,探讨从基因水平鉴别鱼翅制品真伪的方法,提高鱼翅产品标识准确性。
采用不同基因组DNA 提取方法比对,选择适合鱼翅DNA 的提取旗 筛选出C01序列DNA 条形码通用引物,对鱼翅DNA 进行PCR 扩增并测序,应用MEGA 6.0软件对64条鱼翅COZ 序列进行多序列比对,基于邻接法(N-J )构建进化树,分析遗传距离。
同时应用DNAman 构建相应COZ 序列的聚类同源树。
结果表明,本研究所建立的条形码技术能鉴别13种鱼翅物种。
分子系统发育树显示侗_个物种的不同个体基本都能形成单系,节点支持率大都为99% ~ 100%。
聚类分析显示侗一物种都位于同一个分支,不同个体的鱼类的CO/序列相似度均大于95%。
该方法可为广东省流通的鱼翅制品的鉴定提供分子生物 学基础。
关键词:鱼翅;DNA 条形码技术;鉴定中图分类号:S917文献标识码:AIdentification of Species and Authenticity of Shark Fins by DNA Barcoding TechnologyXIONG Juan, HUANG Qihong, CHEN Miner, FANG Jun(Guangdong Provincial Engineering Research Center for Efficacy Component Testing and Risk Substance Rapid Screening of H ealthFood, Guangdong Provincial Key Laboratory of E mergency Test for Dangerous Chemicals, Guangdong Institute of A nalysis ( ChinaNational Analytical Center Guangzhou), Guangdong Academy of Sciences, Guangzhou 510070, China)Abstract: Six species of authentic shark fins in the market were identified by morphology as standard samples, and two kinds of fake shark fins made with gelatin as the main raw material were used as negative controls. The genetic diversity of 35 types of shark fins in the market in Guangdong Province was investigated using DNA barcode technology to probe for a method of identifying the authentic ity of shark fin products from the genetic level with higher accuracy of identification of shark fins products in Guangdong Province. Different genomic DNA extraction methods were compared and the most suitable DNA extraction methods for shark fins were chosen.The universal primers of DNA barcode of COI sequence were screened out, and the DNA of shark fins was amplified and sequenced by PCR. The multiple sequence alignment of 64 shark fin COI sequences was carried out by MEGA 6.0 software. The phylogenetictree was constructed based on the Neighbor-Joining method (N-J ), and the genetic distance was analyzed, and DNAman was used to construct cluster homology tree of COI sequences, with 13 species of shark fin being identified by the DNA barcode technology.Molecular phylogenetic tree showed that diSerent individuals of the same species had basically monophy form, with support rate of 99%〜100% for most of the nodes. Cluster analysis revealed that the same species was located in the same branch, and that the COI se quence similarity of fishes with different individuals was all greater than 95%. In conclusion, this method provides molecular biological basis for identification of shark fins products in Guangdong Province.Key words: shark fin; DNA barcode technology; identification广东省素被誉为养生大省,而鱼翅作为广东著 度水涨船高。
肉桂精准煮散饮片与原饮片的煎煮质量白俊其;苏贺;黄娟;宫璐;李西文;徐江;黄志海;丘小惠【摘要】目的:研究化学指纹图谱及DNA条形码分子鉴定技术在肉桂精准煮散饮片质量体系的应用.方法:应用psbA-trnH序列作为DNA条形码对肉桂进行鉴定;采用标准汤剂煎煮法,比较原饮片及不同规格煮散饮片的出膏率.建立肉桂HPLC指纹图谱,测定指标成分肉桂酸、肉桂醛的含量,同时标定指纹图谱共有峰,比较各样品共有峰相对峰面积及相似度评价.结果:psbA-trnH序列对肉桂药材可实现准确鉴定;精准煮散饮片平均出膏率及煎煮液中指标成分肉桂酸、肉桂醛含量略高于原饮片,且含量差异性明显减小,RSD从原饮片的5.19%、26.80%降低为0.36%、0.42%.10个共有峰相对峰面积均有所升高,均一性明显提高.结论:肉桂精准煮散饮片可提高原饮片的煎煮效率及均一性.%Objective:To study the application of precise powder decoction pieces(PPDP)of Cinnamonum cassia Presl(CCP) with chemical fingerprint chromatography and DNA molecular identification technology were used on quality system.Methods:Ps-bA-trnH sequence was used as DNA barcode to indentify CCP.Different specifications of PPDP were prepared,and their dry ex-tract content rates were compared with that of original slices.HPLC fingerprint of CCP was established and the contents of cinnamic acid and cinnamic aldehyde were mon peak of fingerprint was demarcated.The common peak relative peak areas and similarity evaluation of each sample were compared.Results: CCP could be accurately identified by psbA-trnH sequences. The extract rate of the concentration of cinnamic acid and cinnamic aldehyde of PPDP were slightly higher than that of the originalpieces,and the content differences decreased significantly.RSD of inter-assay dissolution of cinnamic acid and cinnamic aldehyde of the original slices were 5.19%and 26.80%,respectively,which could be reduced to0.36%and 0.42%after mixing and pre-paring into PPDP.The relative peak areas of the 10 common peaks were increased, and uniformity was significantly improved. Conclusion:The precise powder decoction pieces of CCP can improve the extraction efficiency and uniformity of original slices.【期刊名称】《世界中医药》【年(卷),期】2018(013)002【总页数】5页(P468-471,478)【关键词】肉桂;精准煮散饮片;DNA条形码;质量体系【作者】白俊其;苏贺;黄娟;宫璐;李西文;徐江;黄志海;丘小惠【作者单位】广州中医药大学第二附属医院,广东省中医药科学院,中国中医科学院广东分院,广州,510006;广州中医药大学第二附属医院,广东省中医药科学院,中国中医科学院广东分院,广州,510006;广州中医药大学第二附属医院,广东省中医药科学院,中国中医科学院广东分院,广州,510006;广州中医药大学第二附属医院,广东省中医药科学院,中国中医科学院广东分院,广州,510006;中国中医科学院中药研究所,北京,100700;中国中医科学院中药研究所,北京,100700;广州中医药大学第二附属医院,广东省中医药科学院,中国中医科学院广东分院,广州,510006;广州中医药大学第二附属医院,广东省中医药科学院,中国中医科学院广东分院,广州,510006【正文语种】中文【中图分类】R284肉桂为樟科植物肉桂Cinnamonum cassia Presl的干燥树皮。
小麦TaLEC1基因的克隆及其表达特性分析刘豪;王艳丽;孟晓丹;王新国;李永春;任江萍【摘要】为了探讨LEC1基因在小麦(Triticum aestivum L)非生物胁迫应答中的功能,该研究通过RT-PCR结合RACE技术克隆小麦TaLEC1基因,并采用qRT-PCR方法分析了该基因在小麦不同组织以及不同处理下的表达模式,为深入研究小麦LEC1基因在干旱、高温和高盐胁迫下的响应机制奠定基础.结果表明:(1)成功克隆到小麦TaLEC1基因,该基因cDNA序列全长为1 074 bp,其中5'端非编码区23 bp,开放阅读框为741 bp,3'端非编码区310 bp,编码246个氨基酸,具有典型的CBFD_NFYB结构域.(2)实时荧光定量分析显示,TaLEC1在不同组织间表达差异显著,10 d龄幼苗的叶中表达量最高.(3)TaLEC1基因可被植物激素ABA诱导而上调表达,属于ABA依赖型的表达调控通路.(4)PEG模拟干旱胁迫处理后的0.5~1 h,TaLEC1基因呈上调表达;42℃胁迫处理过程中,TaLEC1基因呈稳定上调表达趋势,并在胁迫处理后12h和48 h时表达急剧上调,分别为对照的52.8倍和34.5倍;NaCl胁迫处理0.5h时TaLEC1基因迅速上调表达.研究表明,小麦TaLEC1基因参与ABA依赖的胁迫响应,推测可能在小麦耐受高温胁迫和渗透胁迫过程中发挥着重要的脱水保护功能.【期刊名称】《西北植物学报》【年(卷),期】2019(039)005【总页数】7页(P904-910)【关键词】小麦;LEC1克隆;非生物胁迫;基因表达【作者】刘豪;王艳丽;孟晓丹;王新国;李永春;任江萍【作者单位】河南农业大学农学院,郑州450002;河南农业大学农学院,郑州450002;河南农业大学农学院,郑州450002;河南农业大学农学院,郑州450002;国家小麦工程技术研究中心,郑州450002;河南农业大学农学院,郑州450002;国家小麦工程技术研究中心,郑州450002;河南农业大学农学院,郑州450002;国家小麦工程技术研究中心,郑州450002【正文语种】中文【中图分类】Q785;Q786小麦是中国重要的粮食作物之一,而干旱、低温和高温等是影响小麦生长发育的主要非生物胁迫因子[1-3]。
MEGA 6.60说明书软件界面
菜单的使用:
Data菜单:
●Creat a new:创建一个新的数据比对文件,也就是说当我们比对完一组后,想接着比对
另一组,那么使用它就可以不用退出直接把数据文件导入;
●Open:打开先前已经比对并保存好的文件,它包含两个子菜单:retive sequence from
file和saved aligment session;
●Close: 关闭当前的比对数据文件;
●Save session:保存当前比对结果,可以给比对的结果一个文件名;
●Export alignment:将当前的序列比对结果输出到指定文件,有两种输入格式可供选择:
MGTA 和FASTA.
●DNA sequence:使用它来选择输入的数据DNA 序列,这里需要说明的是如果你输入
的数据是氨基酸序列的话,比对窗口只显示一个标签,若是DNA 序列的话则显示两个标签,一个是DNA 序列的,另一个是氨基酸序列的。
●Protein sequences:选择输入的氨基酸序列,选择后,所以的位点就被当作氨基酸残基
位点来对待。
●Translate/untranslate:只有比对的序列是编码蛋白的DNA序列的时候才可用。
它可以
根据指定的遗传密码表将DNA 序列翻译成特定的氨基酸序列。
●Select genetic code table:使用它将编码蛋白的DNA 翻译成特定的蛋白序列。
●R everse complement:将选择的一整行的DNA 序列变为与之互补配对碱基序列。
●Exit alignment explorer:退出序列比对的资源管理窗口
Edit 菜单:
使用这个菜单可以对我们的比对序列进行想要的一些编辑工作。
●Undo:撤销上一步操作;
●Copy:复制;Cut:剪切;Paste:粘贴;这三个操作都可以只针对一个碱基或氨基酸
残基也可以是一段甚至是整个序列;
●Delete:从比对表格中删除一段序列;
●Delete gaps:去掉序列中的空缺;
●Insert blank sequence:重新插入一空行;标签和序列都是空的;
●Insert sequence from file:从已保存的文件中插入新的序列;
●Select sites:选择一列序列,与点击比对表上方的灰白空格作用类似;
●Select sequence:选择一行序列,与点击比对表格左侧的标签名作用类似;
●Select all:全选;
●Allow base editing:只读保护,只有选择后才能对序列进行编辑操作,否则所以的序列
为只读格式,不能进行任何编辑操作。
Search 菜单:
用来快捷查找序列中的标记未定或者目的碱基或残基。
●Find motif:输入你想要查看的一小段序列。
找到后会以黄色标出;
●Find next:在序列的下游查找目的序列片段;
●Find preious:在序列的上有查找目的序列片段;
●Find marked sites:查找标记位点;
●Highlight motif:突出标记已经选择的位点。
Web 菜单:
●这个菜单提供一个链接Genbank 的入口,可以在网上直接做Blast搜索。
当手上没有
准备好要比对的序列时,可以直接去网上搜索。
●Query gene banks:开启NCBI 的主页;
●Do blast search: 开启NCBI BLAST 主页;
●Show browser:开启网页浏览器。
Sequencer 菜单:
●此菜单下只有一个子菜单:edit sequencer file,用来打开一个打开文件对话框,此对话
框可以打开一个sequencer data file,一旦打开,这个文件就在tracedata file viewer/editor 的对话框中展示出来。
这个编辑窗口允许你查看和编辑automatd DNA sequencer产生的trace data。
它可以阅读和编辑ABI和Staden 格式文件并且序列可以直接被导入到序列比对窗口或被上传到网页浏览器做blast 搜索。
Display 菜单:
这个菜单相对简单,主要用来调整工具栏。
●Toolbars:工具栏菜单,它包含一些子菜单,选择后就会出现在比对的窗口中;
●Use colors:将不同的位点以不同的颜色显示;
●Background color:选择后位点的显示与位点一样的背景颜色;
●Font:字体对话框,通过选择来调整窗口中的序列字符的大小。
Alignment 菜单
●Mark/unmark site:在比对的表格中标记或者不标记一个单一位点,一次每条序列只能被
标记一个位点,不同序列间的位点你可以选择同一列的,也可以是错开的,要根据自己的目的进行选择。
选择标记后的序列可以使用alignmarked sites 进行比对分析。
●Align marked sites: 比对标记的序列,在这里如果在两个或多个序列间标记了不在一列
的位点重新比对后会出现空格。
●Unmarked all sites:把所以标记的位点去标记;
●Delete gap-only site:去掉序同是空格的一列;这在多序列比对前很有用。
●Auto-fill gaps:使用空格补齐不同长度的序列。
建树:下载数据
初步聚类:
建树。