下载文档原格式
Product SpecificationsThermo ScientificExcelsior AS Tissue ProcessorThe Thermo Scientific™ Excelsior has become the byword for quality in tissue processing. It couples high cassette capacity and fast processing with an intuitive user interface and a wealth of safety features.The increasing demand for productivity and efficiency in the laboratory isaddressed in full by Excelsior AS tissue processor. The automated reagent monitoring and in-process reagent rotation minimizes downtime and reagent wastage compared to other tissue processors. Netmon remote monitoring makes support services available to the user in real time, and our xylene-free protocols can improve the laboratory environment. The new improved wax handling system further heightens the Excelsior operator experience.To find out more about the Thermo Scientific Excelsior AS tissue processor please visit our website at/pathologyReagent rotation and quality monitoring With the Excelsior AS tissue processor, alcohol quality is directly monitored using specific gravity, and the operator is alerted when replenishment is required. The unique ‘in process’ rotation of reagents eliminates disruption to your workflow and ensures that unspent reagents are not needlessly discarded nor any reagents overused. This produces significant cost savings as well as saving valuable laboratory staff time. With up to 300 cassettes per processing, the Excelsior AS tissue procesor is the true laboratory workhorse.Safety is a priorityPatented downdraft ventilation protects the operator when the chamber lid is opened. Furthermore, fan assisted ventilation is automatically activated when the reagent cabinet is opened, giving the operator additional protection. Additionally, as the operator does not need to fill or empty bottles, exposure to harmful fumes is avoided.Easier to use, easier to monitorThe sleek new touch screen and intuitive software interface allow easy setup of protocols, and rapid starting via the Daily Operations Mode. Instrument parameters can be remotely operated using Netmon, a system that evens allows the operator to check the instrument status from their home. QC information and instrument event logs are readily downloaded using the on-board USB port.M P A Thermo Fisher Scientificis an ISO Certified companyAnatomical Pathology Tudor Road, Manor ParkRuncorn, WA7 1TAUNITED KINGDOM +44 (0) 800 018 9396 +44 (0) 1928 534 0004481 Campus Drive Kalamazoo, MI 49008 UNITED STATES +1 (800) 522-7270/pathology© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.SPEC001_02a Product SpecificationsThermo Scientific Excelsior AS Features and BenefitsFeatures BenefitsClear and easy-to-read touch screen interface At-a-glance visual check of statusNewly developed intuitive control software Simplifies daily operation222 cassette capacity (300 in random baskets) Maximizes sample throughputAutomated in-process reagent rotation Eliminates manual handling of reagents and extends reagent lifetimeQuality of first reagent alcohol automatically monitored Reagent alcohol is only changed when necessaryThree distinct fill levels in processing chamber Shortens processing timesCircular chamber design with continuous agitation Better reagent circulation and temperature uniformity Patented downdraft ventilation system Protects the operator from fumes when the chamber is opened Potassium permanganate and charcoal filters Additional operator protection from formalin and solventvapoursReaction chamber viewing window Allows visual verification that samples are immersed Systematic logging and reporting of quality control data Historical records of productivity and instrument status arealways availableTen customised programs and five flush options Maximum flexibility for the widest range of tissue samples Suitable for xylene-free processing Reduces staff exposure to harmful chemicals and can improveprocessing qualityBattery back-up with up to four hours operation Allows processing to continue even if the power fails Access code protection To ensure only the designated staff can change settings orprotocolsRemote alarms and LIMS interfacing Keeps the user and the laboratory updatedPhysical and Electrical SpecificationsDimension (D x W x H) 580mm x 710mm x 1370mm (22.8" x 28" x 54")Weight 165 kg / 363 lb (empty); 250kg / 551lb (full)Power supply voltages 100-240 VACFrequency 50 / 60 HzPower 1300 VA (Maximum); 300 VA (Typical)Connections Serial RS232 (LIMS); RJ45 (Netmon)Certifications UL; CSA; IVD; Compliant with 21CFR part 11Ordering InformationExcelsior AS A82300001。
Eliminate Secondary Labeling Steps. Matrix 2D Barcoded ScrewTop Storage tubes are available pre-printed with 1D and human-readable codes that match the tube’s 2D code. Laser etched 2D, linear and human readable barcodes are also included on the sides of the standard latch racks. The additional barcoding on tube and rack eliminates the need for the secondary application of labels, streamlining storage procedures.Enhanced Secure Tracking. A permanently bonded, unique 2D barcode is laser etched onto the base of every tube to securely identify and track samples. The linear and human readable codes match this unique 2D barcode, allowing 1D scanning or visual identification of samples across satellite or collaborative sites without2D readers. Complementary Thermo Scientific 2D and 1D barcode readers scan and instantly decode each tube’s barcode into any application or database. Laser etched 2D, linear, and human readable barcodes are included on three sides of the standard latch rack, providing traceability at the rack level and providing readability to laboratories with or without barcode decoding equipment. The 2D barcode included on the bottom of the rack serves as both an orientation and identification feature for automated and benchtop storage equipment.Superior Storage Format. Matrix 2D Barcoded ScrewTop storage tubes are available in specially designed, barcoded, stackable, microplate-footprint Latch Racks to save precious space in storage and on the bench top while maintaining traceability. Lid canbe positioned to rest on the rack, preventing contamination during manual pipetting. Or remove lid completely for robotic handling. Flexibility. Side-printed Matrix ScrewTop tubes and barcoded latch racks maintain compatibility with existing ScrewTop tube storage systems and accessories. ScrewTop Removal Tool caps/ decaps tubes individually to enable one-handed pipetting. The Thermo Scientific ScrewTop Cap Tray can be used to cap/decap several tubes at once with automated capping systems or to fill tubes on an automated liquid handling platform, then cap an entire rack at once. Seven color cap options allow easy identification of samples at a glance.Stringent Quality Control. Every 2D barcoded storage tube is scanned to ensure readability. All barcodes are checked against our database of previously assigned codes to prevent duplicates.S A M P L E S T O R A G EThermo Scientific™ Matrix™2D Barcoded ScrewTop Tubes in Barcoded RacksMatrix 2D Barcoded ScrewTop Storage tubes are now available in a standard barcoded latch rack. The barcoded latch rack provides permanent 2D, linear,and human readable codes on 3 sidesof the rack. A 2D barcode on the rack bottom enables orientation detection and identification when using automated storage equipment. These additions to the Matrix ScrewTop Storage tube portfolio allow visual sample identification ina range of laboratory procedures and eliminate the need for secondary labeling. Identify and track your samplesfor optimal sample storageProduct SpecificationsThermo Scientific MatrixBarcoded Tubes and RacksDescription 500 µl ScrewTop Storage Tubes 1.0 ml ScrewTop Storage Tubes Item Number3743, 3744, 37453744-WP; 3744-WP1D; 3745-WP; 3745-WP1D 3740, 3741, 37423741-WP; 3741-WP1D; 3742-WP; 3742-WP1D Figure 1A: Tube Side ViewWidth (1A)7.40±0.10 [0.290±0.002]7.40±0.10 [0.290±0.002]Height (1A)30.60±0.20 [1.204±0.007]44.00±0.10 [1.732±0.003]Figure 1B: Tube Side View w/ ScrewTop Width (1B)8.70±0.10 [0.344±0.003]8.70±0.10 [0.344±0.003]Height (1B)39.20 [1.543]52.60 [2.071]Figure 2: Rack Side ViewHeight (2A)29.50±0.10 [1.160±0.005]38.10±0.10 [1.500±0.005]Height (2B)29.10±0.10 [1.145±0.005]42.50±0.20 [1.673±0.008]Height (2C)32.60±0.30 [1.282±0.012]46.00±0.20 [1.809±0.008]Height (2D)41.30±0.40 [1.626±0.017]54.60±0.30 [2.149±0.110]Figure 3: Rack Top View Length 127.75±0.13 [5.029±0.005]127.75±0.13 [5.029±0.005]Width85.46±0.13 [3.364±0.005]85.46±0.13 [3.364±0.005]Figure 4: Stacked Rack ViewSingle Height (4A)44.32±0.24 [1.745±0.010]58.42±0.25 [2.300±0.010]Incremental Height (4B)43.31±0.25 [1.705±0.010]57.30±0.25 [2.220±0.010]Stacked Height (4C)87.63±0.51 [3.450±0.020]114.81±0.51 [4.520±0.020]Description Storage Tubes Barcoded RackItem Number3743-BR, 3744-BR, 3745-BR, 3744-WP-BR, 3744-WP1D-BR, 3745-WP-BR, 3745-WP1D-BR, 3740-BR, 3741-BR, 3742-BR, 3741-WP-BR, 3741-WP1D-BR, 3742-WP-BR, 3742-WP1D-BR, 3748-BR Figure 5: Rack Bottom ViewDistance to barcode from short side (5A)120.48 ± 0.254 [4.74 ± 0.010]Distance to barcode from short side (5B) 1.443 ± 0.203 [0.057 ± 0.008]Barcode length5.83 ± 0.127 [0.230 ± 0.005]Distance to barcode from long side (5C)71.98 ± 0.254 [2.834 ± 0.010]Distance to barcode from long side (5D)8.18 ± 0.203 [0.322 ± 0.008]Barcode width5.30 ± 0.127 [0.209 ± 0.005]Description ScrewTop Cap Tray Item Number 4906, 4477Figure 6: Tray Top View Length 127.75±0.25 [5.03±0.010]Width85.58±0.25 [3.37±0.010]Figure 7: Stacked Tray ViewSingle Height (2A)19.81±0.13 [0.78±0.005]Incremental Height (2B)18.29±0.25 [0.72±0.010]Stacked Height (2C)38.10±0.25 [1.50±0.010]Tube Material Virgin Class VI Medical Grade Polypropylene Rack Material Polycarbonate with acetal latchesCap MaterialVirgin Class VI Medical Grade Polypropylene with Santoprene™ gasket ScrewTop Cap Tray Material Acrylonitrile Butadiene Styrene (ABS)Contaminant-free All tubes and tray are supplied free from DNA, RNAse, DNAse and endotoxinsAutoclavable Racked and unracked tubes are autoclavable with the caps loosened; ScrewTop trays are not autoclavable 2D CodeNon-proprietary, 12x12 Data-matrix with ECC200 Built-in Error Correction Tube/Cap Temp Range -196°C to 121°C; Autoclaving and boiling to vapor phase liquid nitrogen storage Latch Rack Coding 2D Datamatrix; Linear EAN Code 128; Human Readable Matrix Barcoded ScrewTop TubesProduct SpecificationsBarcode WidthDistance to barcodefrom short side (5B)Barcode LengthDistance to barcode from short side (5A)Distance to barcode from long side (5C)s Figure1: Tube Side View Width (1B)s Figure 2: Rack Side ViewSSSST2DTUBES 0615© 2015 Thermo Fisher Scientific Inc. All rights reserved. Santoprene is a registered trademark of Exxon Mobil Corporation. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries./samplestorageCat. No.Non-Barcoded Rack Cat. No.Barcoded Rack DescriptionMax Working Volume, µL Cap Color Qty37443744-BR Tube, ScrewTop, 500 µL 2D, V-Bottom 590Colorless 5 racks of 96/case Sterile3744RED 3744RED-BR Tube, ScrewTop, 500 µL 2D, V-Bottom 590Red 5 racks of 96/case Sterile 3744YEL 3744YEL-BR Tube, ScrewTop, 500 µL 2D, V-Bottom 590Yellow 5 racks of 96/case Sterile 3744BLU 3744BLU-BR Tube, ScrewTop, 500 µL 2D, V-Bottom 590Blue 5 racks of 96/case Sterile 3744GRE 3744GRE-BR Tube, ScrewTop, 500 µL 2D, V-Bottom 590Green 5 racks of 96/case Sterile 3744WHI 3744WHI-BR Tube, ScrewTop, 500 µL 2D, V-Bottom 590White 5 racks of 96/case Sterile 3744PUR 3744PUR-BR Tube, ScrewTop, 500 µL 2D, V-Bottom 590Purple 5 racks of 96/case Sterile 3744AMB 3744AMB-BR Amber Tube, ScrewTop, 500µL 2D, V-bottom 590 5 racks of 96/case Sterile 3743AMB 3743AMB-BR Amber Tube, ScrewTop, 500µL 2D, V-bottom 590Red5 racks of 96/case Sterile 37453745-BR Tube, ScrewTop, 500 µL 2D, V-Bottom, No Caps 590 5 racks of 96/caseSterile 3744-WP 3744-WP-BR Tube, ScrewTop, 500 µL 2D, WP, V-Bottom 590Colorless 5 racks of 96/case Sterile 3744-WP1D 3744-WP1D-BR Tube, ScrewTop, 500 µL 2D, WP/1D, V-Bottom 590Colorless5 racks of 96/case Sterile 3745-WP 3745-WP-BR Tube, ScrewTop, 500 µL 2D, WP, V-Bottom, No caps 590 5 racks of 96/case Sterile 3745-WP1D 3745-WP1D-BRTube, ScrewTop, 500 µL 2D, WP/1D, V-Bottom, No caps 590 5 racks of 96/caseSterile 3743Tube, ScrewTop, 500 µL 2D, V-Bottom 590Colorless Bulk, 480/case Sterile 3743RED Tube, ScrewTop, 500 µL 2D, V-Bottom 590Red Bulk, 480/case Sterile 3743YEL Tube, ScrewTop, 500 µL 2D, V-Bottom 590Yellow Bulk, 480/case Sterile 3743BLU Tube, ScrewTop, 500 µL 2D, V-Bottom 590Blue Bulk, 480/case Sterile 3743GRE Tube, ScrewTop, 500 µL 2D, V-Bottom 590Green Bulk, 480/case Sterile 3743WHI Tube, ScrewTop, 500 µL 2D, V-Bottom 590White Bulk, 480/case Sterile 3743PUR Tube, ScrewTop, 500 µL 2D, V-Bottom590Purple Bulk, 480/case Sterile 37413741-BR Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Colorless 5 racks of 96/case Sterile 3741RED 3741RED-BR Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Red 5 racks of 96/case Sterile 3741YEL 3741YEL-BR Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Yellow 5 racks of 96/case Sterile 3741BLU 3741BLU-BR Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Blue 5 racks of 96/case Sterile 3741GRE 3741GRE-BR Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Green 5 racks of 96/case Sterile 3741WHI 3741WHI-BR Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940White 5 racks of 96/case Sterile 3741PUR 3741PUR-BR Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Purple 5 racks of 96/case Sterile 3741AMB 3741AMB-BR Amber Tube, ScrewTop, 1.0mL 2D, V-Bottom 940Red5 racks of 96/case Sterile 3742AMB 3742AMB-BR Amber Tube, ScrewTop, 1.0mL 2D, V-Bottom 940 5 racks of 96/case 37423742-BR Tube, ScrewTop, 1.0 mL 2D, V-Bottom, No Caps 940 5 racks of 96/caseSterile 3741-WP 3741-WP-BR Tube, ScrewTop, 1.0 mL 2D, WP, V-Bottom 940Colorless 5 racks of 96/case Sterile 3741-WP1D 3741-WP1D-BR Tube, ScrewTop, 1.0 mL 2D, WP/1D, V-Bottom 940Colorless5 racks of 96/case Sterile 3742-WP 3742-WP-BR Tube, ScrewTop, 1.0 mL 2D, WP, V-Bottom, No caps 940 5 racks of 96/case Sterile 3742-WP1D 3742-WP1D-BRTube, ScrewTop, 1.0 mL 2D, WP/1D, V-Bottom, No caps 940 5 racks of 96/caseSterile 3740Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Colorless Bulk, 480/case Sterile 3740RED Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Red Bulk, 480/case Sterile 3740YEL Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Yellow Bulk, 480/case Sterile 3740BLU Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Blue Bulk, 480/case Sterile 3740GRE Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940Green Bulk, 480/case Sterile 3740WHI Tube, ScrewTop, 1.0 mL 2D, V-Bottom 940White Bulk, 480/case Sterile 3740PURTube, ScrewTop, 1.0 mL 2D, V-Bottom940PurpleBulk, 480/caseSterileORDERING GUIDEAsia: Australia: 1300-735-292; New Zealand: 0800-933-966; China +86-21-6865-4588 or +86-10-8419-3588;China Toll-free: 800-810-5118 or 400-650-5118; Singapore +65-6872-9718; Japan: +81-3-5826-1616; Korea +82-2-2023-0640; Taiwan + 886-2-87516655; India: +91-22-6680-3000 Europe: Austria: +43-1-801-40-0; Belgium: +32-2-482-30-30; Denmark: +45-4631-2000; France: +33-2-2803-2180; Germany: +49-6184-90-6000; Germany Toll-free: 0800-1-536-376;Italy: +39-02-95059-554; Netherlands: +31-76-571-4440; Nordic/Baltic/CIS countries: +358-10-329-2200; Russia: +7-(812)-703-42-15; Spain/Portugal: +34-93-223-09-18; Switzerland: +41-44-454-12-12; UK/Ireland: +44-870-609-9203 North America: USA/Canada +1-585-586-8800; USA Toll-free: 800-625-4327South America: USA sales support: +1-585-586-8800 Countries not listed: +49-6184-90-6000 or +33-2-2803-2000Sterile ScrewTop Tube Caps Cat. No.DescriptionCap Color Qty/Case Cat. No.Description Cap Color Qty/Case 4906Tray, ScrewTop Cap, Empty 5 trays4470Caps, ScrewTop Tube Colorless Bulk, 5004477Tray, ScrewTop Cap Coloreless 5 trays of 96 caps 4470RED Caps, ScrewTop Tube Red Bulk, 5004477RED Tray, ScrewTop Cap Red 5 trays of 96 caps 4470YEL Caps, ScrewTop Tube Yellow Bulk, 5004477YEL Tray, ScrewTop Cap Yellow 5 trays of 96 caps 4470BLU Caps, ScrewTop Tube Blue Bulk, 5004477BLU Tray, ScrewTop Cap Blue 5 trays of 96 caps 4470GRE Caps, ScrewTop Tube Green Bulk, 5004477GRE Tray, ScrewTop Cap Green 5 trays of 96 caps 4470WHI Caps, ScrewTop Tube White Bulk, 5004477WHI Tray, ScrewTop Cap White 5 trays of 96 caps 4470PURCaps, ScrewTop TubePurpleBulk, 5004477PURTray, ScrewTop CapPurple5 trays of 96 caps。
RevertAid第一链cDNA Synthesis试剂盒#K1621, #K1622分析证明书质量控制#K1621Lot采用100 fg对照GAPDH RNA和对照引物进行RT-PCR反应，通过在1%琼脂糖上进行凝胶电泳和溴化乙锭染色显示得到足够量的496 bp的产物质量认证人：Jurgita Zilinskiene目录页码试剂盒组成 (2)存储条件 (2)产品说明 (2)注意事项 (3)操作步骤 (6)RT-PCR (6)合成cDNA用于克隆………..………………………………………7 实验对照……………………………………………………………….8 问题分析与解决 (10)** 一个单位的RiboLockRNase酶抑制剂抑制5ng RNA酶A 50%的活性。
存储条件试剂盒中所有组分应存储在-20°C。
对照用RNA可存储于-70°C以便长期使用。
产品说明RevertAid第一链cDNA合成试剂盒以mRNA或者总RNA为模板，高效合成第一链cDNA。
本试剂盒使用RevertAid M-MuLV反转录酶，它的RNA酶H的活性与AMV反转录酶相比较低。
该反转录酶可耐受42-50°C温度，合成的cDNA片段长度达13kb。
试剂盒中含有RiboLock 重组RNA酶抑制剂，防止RNA降解，可耐受55°C高温。
试剂盒同时含有oligo(dT)18和随机六聚体引物。
随机六聚体引物与模板非特异性地结合，以总RNA中任何RNA为模板合成cDNA。
oligo(dT)18选择性和RNA 3’po ly(A)配对结合，只以有poly(A)尾巴的mRNA为模板合成cDNA。
使用本试剂盒也可采用序列特异性引物。
合成的第一链cDNA能直接用作PCR或荧光定量PCR的模板，第二链cDNA的合成或线性RNA扩增，也可用于需要用带有放射性或非放射性核苷酸标记第一链cDNA的实验，比如将标记好的第一链cDNA 作为杂交实验中的探针或者用于微阵列分析。
Thermo ScientificRevco High-PerformanceLaboratory Refrigeratorsand FreezersUnrivalled cold storageprotection for your valuablesamples and materialsChromatographyGeneral PurposeBlood BankPlasmaPharmacyEnzymeThermo Scientific Revco High-Performance Lab Refrigerators & FreezersTemperature Range: +1°C to +8°C, factory preset to +4°C (auto defrost)Int. Dimensions*Ext. Dimensions*Cu.Ft.Voltage Amps/Adjustable H x D x W H x D x W Ship Weight Model No.(L)(Hz)Breaker (Plug)Cabinet Door Shelvesinches (mm)inches (mm)lb (kg)REL404A 4.9115 (60)8.0/15(P1)undercountersingle,320.0 x 20.0 x 20.034.0 x 28.9 x 24.0212 (96)REL404D (133)208/230 (60) 6.0/15 (P4)solid(508 x 508 x 508)(864 x 734 x 610)REL404V 230 (50) 6.0/15 (P7)REL404W 230 (50) 6.0/15 (P8)REL1204A 11.5115 (60)9.4/15 (P1)space single,452.4 x 21.0 x 20.073.6 x 30.0 x 24.0280 (127)REL1204D (326)208/230 (60) 6.0/15 (P4)saver solid(1331 x 533 x 508)(1869 x 762 x 610)REL1204V 230 (50) 6.0/15 (P7)REL1204W 230 (50) 6.0/15 (P8)REL2304A 23.3115 (60)9.4/15 (P1)uprightsingle,458.0 x 29.0 x 24.079.2 x 37.2 x 28.0405 (184)REL2304D (659)208/230 (60) 6.0/15 (P4)solid(1473 x 737 x 610)(2012 x 945 x 711)REL2304V 230 (50) 6.0/15(P7)REL2304W 230 (50) 6.0/15 (P8)REL3004A 29.2115 (60)9.4/15 (P1)uprightsingle,458.0 x 29.0 x 30.079.2 x 37.2 x 34.0435 (197)REL3004D (826)208/230 (60) 6.0/15 (P4)solid(1473 x 737 x 762)(2012 x 945 x 864)REL3004V 230 (50) 6.0/15(P7)REL3004W 230 (50) 6.0/15 (P8)REL4504A 45.8115 (60)15.0/20(P2)uprightdouble,858.0 x 26.0 x 52.5 79.1 x 34.5 x 56.5 670 (304)REL4504D (1297)208/230 (60)8.5/15 (P4)sliding glass(1473 x 660 x 1334(2009 x 876 x 1435)REL4504V 230 (50)8.5/15 (P7)REL4504W 230 (50)8.5/15 (P8)REL5004A 51.1115 (60)15.0/20(P2)uprightdouble,858.0 x 29.0 x 52.579.2 x 37.2 x 56.5585 (265)REL5004D (1447)208/230 (60)8.5/15 (P4)solid(1473 x 737 x 1334)(2012 x 945 x 1435)REL5004V 230 (50)8.5/15 (P7)REL5004W 230 (50)8.5/15 (P8)REL7504A 78.8115 (60)16.0/20(P2)uprighttriple,1258.0 x 29.0 x 81.079.2 x 37.2 x 85.0805 (365)REL7504D (2231)208/230 (60)10.5/15 (P4)solid(1473 x 737 x 2057)(2012 x 945 x 2159)REL7504V 230 (50)10.5/15 (P7)REL7504W230 (50)10.5/15 (P8)Laboratory RefrigeratorsR E F R I G E R A T O R SOur selection of high performance, laboratory refrigerators is available in a wide range of cabinet sizes to fit virtually any application need. Each refrigerator combinesadvanced microprocessor temperature control with the highest quality cabinet construction and alarm systems.Positive-forced air circulation maintains temperature uniformity to protect your valuable samples and inventory.F R E E Z E R SOur high performance, laboratory freezers are designed for medical and scientific applications requiring storage of reagents, pharmaceuticals, biologicals, and other commonly used laboratory materials. A range of models offers cabinet sizes to fit your specific space needs and storage volume requirements, from undercounter units to full size models.A dditional features included in both refrigerators and freezers:•1” insulated access port standard•Positive forced-air circulation maintains temperature uniformity •Set of four 2” casters: two locking and two regular•Environmentally friendly, mercury-free LED interior lighting (lab refrigerators only)•Adjustable shelves14Temperature Range: -30°C (auto defrost)Int. Dimensions*Ext. Dimensions*Cu.Ft.Voltage Amps/Adjustable H x D x W H x D x W Ship Weight Model No.(L)(Hz)Breaker (Plug)Cabinet Door Shelvesinches (mm)inches (mm)lb (kg)ULT430A 4.9115 (60)8.0/15(P1)undercountersingle,320.0 x 20.0 x 20.034.0 x 28.9 x 24.0212 (96)ULT430D (133)208/230 (60) 6.0/15 (P4)solid(508 x 508 x 508)(864 x 734 x 610)ULT430V 230 (50) 6.0/15 (P7)ULT430W 230 (50) 6.0/15 (P8)ULT1230A 11.5115 (60)8.0/15 (P1)space single,452.4 x 21.0 x 20.073.6 x 30.0 x 24.0280 (127)ULT1230V (326)230 (50)8.0/15 (P7)saver solid (1331 x 533 x 508)(1869 x 762 x 610)ULT1230W 230 (50)8.0/15 (P8)ULT2330A 23.3115 (60)16.0/20(P2)uprightsingle,458.0 x 29.0 x 24.079.2 x 37.2 x 28.0370 (168)ULT2330D (659)208/230 (60)12.0/15 (P4)solid(1473 x 737 x 610)(2012 x 945 x 711)ULT2330V 230 (50)12.0/15(P7)ULT2330W 230 (50)12.0/15(P8)ULT3030A 29.2115 (60)16.0/20(P2)uprightsingle,458.0 x 29.0 x 30.079.2 x 37.2 x 34.0445 (202)ULT3030D (826)208/230 (60)12.0/15 (P4)solid(1473 x 737 x 762)(2012 x 945 x 864)ULT3030V 230 (50)12.0/15(P7)ULT3030W 230 (50)12.0/15(P7)ULT5030A 51.1115 (60)16.0/20(P2)uprightdouble,858.0 x 29.0 x 52.579.2 x 37.2 x 56.5600 (272)ULT5030D (1447)208/230 (60)12.0/15 (P4)solid(1473 x 737 x 1334)(2012 x 945 x 1435)ULT5030V 230 (50)12.0/15 (P7)ULT5030W230 (50)12.0/15 (P8)Laboratory Freezers* Published interior volumes are nominal; useable cu.ft. (L) capacities may be offset by shelves, drawers, blower coils, or cabinet protrusions. For specific cabinet dimensions and allowances, or for verification of clearance tolerances, contact our sales representative. Product performance may be affected by ambient temperature conditions, humidity conditions, sample loading preferences, and frequency of door openings. Contact our sales representative for specific inquiries regarding your application.Temperature Range: -20°C (manual defrost)Int. Dimensions*Ext. Dimensions*Cu.Ft.Voltage Amps/Adjustable H x D x W H x D x W Ship Weight Model No.(L)(Hz)Breaker (Plug)Cabinet Door Shelvesinches (mm)inches (mm)lb (kg)UGL2320A 23.3115 (60)13.5/20 (P2)uprightsingle,458.0 x 29.0 x 24.079.2 x 37.2 x 34.0425 (193)UGL2320D (659)208/230 (60) 5.6/15 (P4)sold(1473 x 737 x 610)(2012 x 945 x 711)UGL2320V 230 (50) 5.6/15(P7)UGL2320W230 (50)5.6/15(P8)Control Panel:Keyed on/off switch with set point security Microprocessor control system with audio and visual alarms Bright, digitaltemperature displayOptional, inkless, seven day, graphic, chart recorderDoors:Spring-loaded, self closingdoors with 90° stay-open feature Lockable doors for added sample securityOptional glass doors (lab refrigerators)Cabinets shown: 23.3 cu. ft. single door (REL2304) lab refrigerator and 51.1 cu. ft. double door (ULT5030) lab freezer15。
Thermo Scientific Sorvall Primo and Primo RRotor FlexibilitySix fixed angle, swinging bucket and microliter rotors for all common tubes from 0.2 to 100 mL are available for appli-cations using microliter, DIN, blood sam-ple and disposable conical tubes. Additionally, a 12-place swinging bucket rotor for micro tubes is available for the Sorvall Primo R.User-Friendly TechnologyThe automatic rotor recognitionsystem identifies the rotor being used and allocates individual parameters to improve lab efficiency. Rapid acceleration and braking times minimize waiting and ensure rapid sample processing. Low noise levelsduring operation provide a more comfort-able working environment.Imbalance RecognitionThe Sorvall Primo series is equipped withelectronic imbalance recognition. Not onlydoes it react if the centrifuge is loadedincorrectly, but it also registers imbal-ances during a run caused, for example,by tube breakage. Centrifugation is inter-rupted and the user can remove the rotorand, if necessary, decontaminate it.User ComfortThe Thermo Scientific EASYcontrol II userpanel clearly displays all parameters. Selfexplanatory symbols, which are interna-tionally recognized, and the clear arrange-ment of function keys help you avoidincorrect operation. The time and speedparameters are set with the easy-to-cleantouch panel. Two acceleration and ninebraking curves are available for sensitivesamples.Our compact high-performance Thermo Scientific Sorvall Primo and Primo R centrifuges offer the functionality of three centrifuges. The Sorvall®Primo, and its refrigerated counterpart, the Sorvall Primo Rare microliter, high speed and general purpose centrifuges all at the same time.Thermo Scientific Sorvall Primo and Primo R Rotors and AccessoriesHighconic ®Rotor 45°Max. capacity mL 6 x 50Max. speed rpm 8,500Max. RCF x g 10,015Acceleration time s 41Braking time s 30Max. radius cm 12.4Min. temperature(only Sorvall Primo R 1))°C –1°Cat No.750075881) At max. speed and 23°C room temperature Adapter for Highconic Rotor 75007588Adapter Max. tube size Tubes Rotor Cat No.(mm)per Length Adapter 1.5 mL micro tubes 1157424760029053.5 mL 11100424750030916.5 mL 131132127500309212 mL 16952127500309316 mL 18122167600290638 mL 25112167500309450 mL 29122167500310215 mL disposable conical 16.5120167500309550 mL disposable conical 1)3011716750031031) Included in the price of the rotor Swinging Bucket Rotor Max. capacity mL 4 x 100Max. speed rpm 4,000Max. RCF x g 2,585Acceleration time s 22Braking times 20Max. radiuscm 14.5Min. temperature(only Sorvall Primo R 1))°C –9°Cat No.Rotor 75007591Round bucket 750075551) At max. speed and 23°C room temperature Adapter for Swinging Bucket Rotor 75007591Adapter Max. tube size Tubes Rotor Cat No.(mm)per Length Adapter 1.5/2 mL micro tubes 11421040750075475 mL blood sample 1375520750035997 mL DIN 12102520750075452)7 mL blood sample 13105312750075462)10 mL blood sample 161004167500359815 mL DIN/blood sample 17104312750075442)25 mL DIN 25110147500754350 mL DIN 351051475007542100 mL 4410014–15 mL disposable conical 16.5120147500755750 mL disposable conical 0301171475007556Aerosol-tight cap for round bucket 750075982) Distance pads must be used for tubes shorter than 80 mm (pack of 12, Cat No. 76003266)Drum Rotor Max. capacity mL 80 x 1.5/2Max. speed rpm 12,000Max. RCF x g 14,005Acceleration time s 31Braking time s 20Max. radiuscm 8.7Min. temperature(only Sorvall Primo R 1))°C 4°Cat No.750075951) At max. speed and 23°C room temperature Racks for Drum Rotor 75007595Racks Tu b es Rotor Color Cat No.per Adapter 1.5 mL microliter tubes 1080Yellow 760014991.5/2 mL microliter tubes 1080Red 760012441.5/2 mL microliter tubes (60°)648Gray 750014980.3 mL micro capillary tubes 864Blue 760012460.5/0.6 mL microliter tubes 15120Green 760012470.25/0.4 mL microliter tubes 20160Yellow 76001248PCR strips with 8 tubes 312Nature 76001587750075887500759575007591Thermo Scientific High conic Rotor•Fixed angle rotor for 6 x 50 mL with max.10,000 x g•The angle of 45° ensures that samplematerial does not run out and the pelletforms at the bottom of the tube•Adapter for highspeed tubes from 1.5 to50 mL and 15 or 50 mL disposableconical type tubes Swinging Bucket Rotor•A round bucket for 4 x 100 mL max•Adapters for all types of tubes such asDIN, blood sample, disposable conicaland micro tubes from 1.5 to 100 mL•An aerosol tight cap is availableas an option Drum Rotor•Suitable for high capacities of up to 80microlitre tubes•Reduced braking and acceleration times•Racks for every volume•A 60° angle rack for 1.5 mL tubes makesoperations easier•PCR rack for 3 “PCR strips” with 8 tubesIndicates that the centrifuge accessory has been successfully tested for biological containment.7500759375007592Trust Thermo Scientific NALGENE and NUNC cen-trifuge bottles and tubes to deliver quality and per-formance. These products are in conformance withISO 13485: 2003, cGMP and USP Class VI; you’ll findupdated speed ratings and lot specific product cer-tificates online. Available in a variety of resins, withcapacities from 10mL to 2L, Nalgene®and Nunc®consumables are guaranteed a perfect fit withThermo Scientific centrifuges and rotors.Sorvall Primo and Primo R,the ideal centrifuge for:•Small medical laboratories•Biochemistry and molecularbiological research•Routine analysis in industriallaboratoriesSorvall Primo R。
产品信息Thermo ScientificMaxima第一链cDNA合成试剂盒（适用于RT-qPCR）#K1641，#K1642/onebio分析证书经验证，在两步法RT-qPCR中，对于不同起始量（5 ng-0.5 fg）的RNA逆转录产物，使用Thermo Scientific Maxima SYBR Green/ROX qPCR 预混液进行qPCR扩增。
其反应效率在90-110％之间，曲线斜率在-3.09和-3.58之间，且相关系数>0.99。
质量授权人：Jurgita Zilinskiene目录页码试剂盒组分 (4)贮存条件 (4)产品描述 (4)重要提示 (5)RT-qPCR实验方案 (6)对照反应 (6)疑难解答 (7)参考文献 (8)试剂盒组分贮存条件所有试剂盒成分都应贮存于-20 °C。
产品描述Thermo Scientific Maxima第一链cDNA合成试剂盒是合成cDNA第一链的方便系统，适用于两步法实时定量RT-PCR（RT-qPCR）中的cDNA合成。
试剂盒采用先进的Maxima 反转录酶（RT），该酶源自M-MuL V 逆转录酶的体外进化。
这种酶相比M-MuL V逆转录酶热稳定性高，扩增效果强劲且具有更高的cDNA合成效率。
Maxima第一链cDNA合成试剂盒能够在较高温度下（55-65℃），在很宽的总RNA量范围内（从1 pg到5 μg）合成cDNA。
合成反应能在15-30分钟内完成。
Maxima第一链cDNA合成试剂盒的各组分经预混合，以节约时间和减少移液错误。
试剂盒由Maxima Enzyme Mix、5×反应混合液、无核酸酶的水三部分组成。
Maxima Enzyme Mix包含Maxima反转录酶和Thermo Scientific Ribolock RNase抑制剂。
重组的Ribolock RNase抑制剂能有效保护RNA模板在高于55℃时不被RNase A、B和C降解。
Nantong ShanghaiDarmstadtIrvineLenexaSt. LouisSongdoLiquid Centers St.Louis, MO USA Irvine, Scotland, UK Nantong, ChinaDry Powder Media Centers Lenexa, KS USAIrvine, Scotland, UK Darmstadt, GermanyimMEDIAte ADVANTAGE ® Centers Lenexa, KS USA St.Louis, MO USA Nantong, ChinaSongdo, South KoreaManufacturing plant Redundant supply facility New manufacturing facility R&D centers for cell culture media & related productsMANUFACTURING LOCATIONSKnowing your cell culture mediaLack of consistency in cell culture media can create significant variation in your upstream process. Our supply chain experts are committed to providing you with the highest quality and most consistent cell culture media — regardless of manufacturing site location.Understanding Trace Element VariabilityGLOBAL QUALITY MANAGEMENTRAW MATERIALSMANUFACTURING CAPABILITIESThe role of trace elements and the impact on protein and product quality is well documented. We offer the right data to begin understanding this variability.• Quantitative testing by ICP-MS • Cu, Mn, Zn, Mo, Ni, V, Al, Se, Cr, Co • Results reported on the CofAGlobal Raw Materials VendorManagement ProgramMaterial QualificationSupplier QualificationChange NotificationGlobal ManufacturingNetworkGeneration of Qualified Raw Materials List Procurement from Qualified Raw Materials ListA cornerstone of our focus, our global program for the control, management, and procurement of raw materials provides theconfidence you need for consistent, quality product.High-quality products, comprehensive documentation, and superior customer support for qualification, risk assessment, and process optimization. Now available for a range of cell culture media.Quality ControlStandard testing with options to add customized quality testing.Finished Product Testing MethodologyNA EU ChinaAppearance Uniformity/color • • •pHUSP 791 • • • OsmolarityUSP 785 • • •Bioburdon (Powder) USP 61 • • •Sterility (Liquid) USP 71 • • •Endotoxin USP 85 • • •(Kinetic, Chromogenic, Gel clot LAL)Redundant Supply FacilitiesWe know the challenges global biopharma companies can have with securing dual source of cell culture media. We provide the solution by offering two sister sites-same technology, single source of raw materials and global quality management to deliver dual sourcing from one supplier.Irvine, UKLenexa, KS, US Custom Capabilities Small Scale imMEDIAte ADVANTAGE ® Services• Pre-GMP product intended for bioprocess development studies • Liquid; 1-200L batch sizes• Powder: 0.5 - 20kgs batch sizesCustom Capabilities Large Scale• Clinical to commercial supply • Liquid: 50 - 10,000 L batch sizes • Dry powder: 25-6000 kg batch sizesRaw MaterialsOption to use unique,non-qualified raw materialsfor your experimentsRight Technology & ProcessesEquivalent liquid and dry powder manufacturing methods as compared to large scale facilitiesDocumentation to SupportCertificate of analysisand safety data sheet with each batch Quality Services: Customer InquiriesGlobal Quality Management SystemOur cell culture media sites are certified as ISO 9001:2015 * Voluntarily comply to the Joint IPEC-PQG Guide on GoodManufacturing Practices for Excipients and applicable sections of Annex 1of the EU Guidelines to Good Manufacturing Practice for Medicinal Products. Apply to Lenexa, St. Louis-Broadway, Irvine, Darmstadt, and Nantong (liquid) sites.NEW! IPEC-PQG compliance*Design SupportLet one of our R&D teams or our designs specialist help you develop your customcell culture media.Our global network of manufacture sites are specifically designed and operate to exceed industry standards for cell culture media intended for bioprocesses.Front Line ContactProduct documentation,Q&A TIER1Technical & Quality Workflows Appropriate team support TIER2Expert SupportScientific or site expert supportTIER3NEW! Emprove ®Cell Culture MediaPharma & Biopharma Raw Material SolutionsThe life science business of Merck operates as MilliporeSigma in the U.S. and Canada.Merck, SAFC, Emprove, imMEDIAte ADVANTAGE, and the Vibrant M are trademarks of Merck KGaA, Darmstadt, Germany and/or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessibleresources. © 2020 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.Lit. No. MK_FL6630EN Ver 1.0 10/2020/emproveLearn more/ccmsupply。
Thermo Scientific ThermoChill Series Recirculating ChillersPerformance, value, simplicityThe Ideal FitT emperature control products for a variey of applications As an innovative leader in temperature control, wehave the expertise to help enable you to optimizeyour liquid cooling and heating applications whileincreasing productivity and reducing operatingcosts. With over 50 years of service and countlesssuccessful installations around the world, we collaboratewith you to provide product and applications expertiseto meet the most demanding temperature controlrequirements. From bio-tech and pharmaceutical toprinting and semiconductor industries, companies aroundthe world continue to make Thermo Scientific temperaturecontrol products their ideal choice.Typical Applications:•Analytical •Automotive •Biotech •Laboratory•Laser•Packaging•Printing•Small Industrial•UniversitySelect the product that is right for your applicationfrom a comprehensive portfolio of temperature controlsolutions that deliver scalable product offerings rangingfrom bench top research to large process manufacturing.Our new and innovative products – developed fromcustomer feedback – represent a breakthrough inperformance, configurability and technology that providethe following advantages:A Flexible Choice: We have options to help you configurea flexible, cost-effective temperature control system thatsuits your specific requirements.Innovation: Our research and development team isfocused on designing innovative products based on yourfeedback.Global Service and Support: With our extensive globalfootprint, as well as, service and support capabilities, wecan support you anywhere in the world.Thermo Scientific ThermoChill recirculating chiller seriesPerformance: with your choice of cooling and pumping capacities, and a wide temperature range, select the performance level your application requires.Value: when you need reliable cooling support for your application, this powerful recirculating chiller offers ahigh quality, cost effective solution.Simplicity: innovative and intuitive controller that is easy to use, and a plug-and-play design that allows you to be up and running in minutes.Performance, value and simplicityCooling Lightilluminates when this chilleris cooling Setpointilluminates when setpoint isbeing adjusted Low Temp Alarm illuminates during adjustment or alarm High Temp Alarm illuminates during adjustment or alarmCommunications illuminates when serial communication is enabled(optional)Pressure Gauge for easy viewing of application pressureDigital Display indicates setpoint and displays additional operating values with menu selectionTouchpad Data Entry adjusts controller valuesEnter Button accesses menus and confirms settingsPower ButtonLocking casters for easy maneuveringAccessible fill port for easy fillingIntuitive digital controller with controllerindicator lights (see diagram below)RS232 digital communications optional(located on the back of the chiller)Pressure gauge located on control panelfor easy viewing of application pressurePerformance curvesCooling Capacity for ThermoChill I, II, & IIIPumping Capacity for Positive Displacement Pumps (PD-1 and PD-2)Pumping Capacity for Magnetically Driven Centrifugal Pump (MDC1)Cooling capacity based on chillers with PD-1 pumps with no backpressure. Other pumps will affect cooling capacity performance. Pressure values are differential pressures between the inlet and the outlet of the chiller. Specifications subject to change. C ooling capacity obtained using water between 8 °C to 30 °C and 50/50 Ethylene Glycol/water below 8 °C. Glycol water mixtures are required below 8 °C in order to prevent freezing of the cooling coils. Failure to follow these directions will result in a loss of cooling capacity and potential damage to the chiller.ACBTemperature ºC ºF4050607080-101030203050 HzA = ThermoChill IIIB = ThermoChill IIC = ThermoChill I20o C o l i n g C a p a c i t y800060004000200000BTU/Hr Watts 2500200015001000500AB A = PD-1B = PD-27.010*******60504030201006.05.04.03.02.01.0000123455101520P r e s s u r eBARPSIDFlow Rate LPM GPM50 HzAB CTemperatureo C o l i n g C a p a c i t y800060004000200000BTU/HrWatts 2500200015001000500ºC ºF4050607080-10100302060 Hz3020A = ThermoChill IIIB = ThermoChill IIC = ThermoChill IAB7.010090807060504030201006.05.04.03.02.01.00LPM GPM00123455101520P r e s s u r eBARPSIDFlow RateA = PD-1B = PD-260 Hz241.5050 Hz & 60 HzBARPSID LPM0 2.55.07.510.012.515.017.520.0GPM012345Flow RateP r e s s u r e1.251.000.750.500.252016128400Innovative designThe ThermoChill series of chillers is a compact line of refrigerated recirculators offering cooling capacities up to 2000 watts. Innovative design features such as minimized plumbing connections and an integrated full-flow fluid strainer maximize product reliability.Three Pump OptionsPositive Displacement Pumps (PD-1/PD-2) deliver a consistent flow over a wide pressure range making them appropriate for use with high back-pressure applications. Adding the external pressure reducer (EPR) accessory allows safe use with pressure sensitive applications.Magnetically Driven Centrifugal Pump (MDC1) deliver high flow rates at low pressure making them ideal for use with laboratory glassware and other low back-pressure applications.SpecificationsSpecifications obtained at sea level using water as the recirculating fluid, at a 20 °C process setpoint, 20 °C ambient condition, at nominal operating voltage. O ther fluids, process temperatures, ambient temperatures, altitude or operating voltages will affect performance.1 UL listing applies to 60 Hz ThermoChill models only.External Pressure Reducer (EPR) AccessoryThis accessory attaches to the chiller to limit the maximum outlet pressure of the chiller. Choose this accessory when circulating to applications that are sensitive to higher pressures or when circulating through glass.ThermoChill Plumbing KitThe plumbing includes (2) ½-in x ½-in MPT fittings, 25 feet of 5/8-in ID Polybraid hose, (2) hose clamps & hose insulation. The plumbing kit can be used across all ThermoChill models.Need More Capacity?The Thermo Scientific™ Polar, Thermo Scientific™ ThermoFlex™ , and Thermo Scientific™ Merlin™recirculating chillers have additional temperature ranges, cooling capacities, and pumping capacities available to meet your specific application requirements.Visit /tempcontrolselector for more information.Please see contact information below, if you need assistance selecting a chiller and to discuss your application.Ordering InformationFind out more at /tc© 2016 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. BRTCTHERMOCHILL 1116Australia +61 39757 4300China +800 810 5118France +33 (0) 1 60 92 48 00Germany +49 (0) 721 4 09 44 44India +91 22 6716 2200Italy +39 02 95059 552Japan +81-3-6832-9270Netherlands +31 76 579 55 55New Zealand +64 9 980 6700Nordic/Baltic/CIS countries +358 10 329 2200Spain/Portugal +34 93 223 09 18UK/Ireland +44 870 609 9254USA/Canada toll free +1 (800) 258-0830USA +1 (603) 436-9444Countries not listed +49 6184 90 6000North America ***************************Europe ****************************China *************************India ******************************Japan ****************************。
Thermo Scientific TM TDE 系列超低温低温箱安装和操作332851H62 • 修订版 A • 2022 年 8 月重要事项请阅读本安装和操作手册。
如果不遵守本手册中的说明，可能会导致设备损坏、操作人员受伤以及设备性能下降。
注意所有内部调整和维护必须由符合所需资格的服务人员进⾏。
本手册中的材料仅供参考。
手册内容及其所包含的产品信息如有变更，恕不另⾏通知。
Thermo Fisher Scientific 对本手册不作任何声明或保证。
对于任何由于使用此手册而引起的直接或间接损坏，Thermo 不承担任何责任。
© 2022 Thermo Fisher Scientific Inc。
保留所有权利。
目录型号 (1)安全考量因素 (2)符号术语 (3)拆包 (5)附件 (5)常规建议 (6)温度监测系统 (6)日常使用 (6)首次加载 (6)格栅门打开/关闭 (6)操作标准 (7)电气规格 (7)安装 (8)位置 (8)保护性导体电流 (8)布线 (8)调平 (9)刮冰刀 (9)备用系统（选配） (9)门操作 (9)安装远程报警连接器 (9)适用范围 (9)操作 (10)初始启动 (10)操作概述 (10)显示画面 (10)设置 (11)关机 (12)刮冰器说明 (13)用途 (13)备用系统（选配） (14)CO2 和 LN2 的预防措施 (14)安装 (14)启动 (15)操作 (15)温度记录仪（选配） (16)设置和操作 (16)更换图表纸 (16)校准调节 (16)维护 (17)清洁冷凝器 (17)清洁冷凝器过滤网 (17)门封维护 (17)低温箱除霜 (17)电池维护 (17)维护计划 (18)故障排除指南 (19)错误代码 (23)保修 (24)保修（国际） (25)附录A：报警汇总 (26)附录B：Modbus ASCII 参数表 (28)临终关怀 (36)WEEE 合规性 (37)联系信息 (39)型号表格 1. 适用型号TDECxxx86F*063/252/396A/D/V/L/K通用型Thermo Fisher Scientific ULT Freezers 型号 | 12 | 安全考量因素Thermo Fisher Scientific ULT Freezers安全考量因素本手册将采用以下符号规则：以下是可能在本产品上使用到的符号和安全警告：（⻅第 5 和第 6 ⻚的列表）以下是本产品的重要安全事项：此符号单独使用时指示会降低人员受伤⻛险或装置性能不良⻛险的重要操作说明。
9/3/10Table of ContentsSafety information (3)Important Information (3)Alert Signals (3)Introduction (4)General Usage (4)Principles of Operation (4)Installation (4)General Specifications (5)Environmental Conditions (5)Declaration of Conformity (5)Operation (6)Precautions and Limitations (6)Maintenance and Servicing (7)General Cleaning Instructions (7)Exploded View (8)Replacement Parts Listing (9)Ordering Procedures (10)One Year Limited Warranty (11)23Your Thermo Scientific Speci-Mix Test Tube Specimen Mixer has been designed with function,reliability,and safety in mind.It is your responsibility to install it in con-formance with local electrical codes.For safe operation,please pay attention to the alert signals throughout the manual.Important InformationThis manual contains important operating and safety information.You must carefully read and understand the contents of this manual prior to the use of this equip-ment.SafetyInformationWarningWarnings alert you to a possibility of per-sonal injury.CautionCautions alert you to a possibility of damage to the equipment.NoteNotes alert you to pertinent facts and conditions.Alert Signals4The Speci-Mix Test Tube Specimen Mixer provides gentle but thorough mixing of in vitro diagnostic specimens.The specimens are placed in the grooves of the white silicone pad,which is mounted on the platform.The platform rocks at a constant speed to provide gentle and thorough mixing of test tube contents.General UsageDo not use this product for anything other than its intended usage.Principles of Operation A synchronous gear motor drives a cam and linkage as-sembly that is attached to the rocker plate,which rocks in a cyclic 48°maximum motion at 18rpm.InstallationRemove the Speci-Mix from the carton.Check electrical specifications and plug into a properly grounded recepta-cle.IntroductionNoteWith a 240V/60Hz power supply the speed is 20rpm,with a 240V/50Hz,16.5rpm.Thermo Fisher ScientificRobert-Bosch-Strasse 1,D-63505 Langenselbold,Germany6Place tubes in the grooves of the white silicone pad.Center tubes for balanced mixing.On the side without ridges,the reversible pad will accept tubes over 125mm in length.The side with ridges will accept tubes less than 125mm in length.Turn the switch to the “on”position to start the mixing.Precautions and Limitations1.Do not overload by weight.Loads over 1-1/4pounds may cause jerky rocking action.2.Wash white silicone pad with soap and water and rinse when needed to assure the friction-grip quality of the pad.OperationWarningTo avoid personal injury,do not use in the presence of flammable or combus-tible chemicals —fire or explosion may result.This device contains compo-nents that may ignite such materials.NoteIf the equipment is used in a manner not specified by the manufacturer,the protection provided by the equip-ment may beimpared.71.The drive motor and linkage assembly do not require lubrication.The drive motor is imped-ance protected.2.Jerky rocking action without a load indicates motor replacement is necessary.3.Failure of the unit when plugged in and with the toggle switch in the “on”position indicates de-fective toggle switch,malfunctioning drivemotor or disengaged linkage.Remove right end cap to observe.General Cleaning InstructionsWipe exterior surfaces with lightly dampened cloth con-taining mild soap solution.Maintenance andServicingWarningTo avoid electrical shock,always dis-connect from power supply before maintenance and servicing.Refer ser-vicing to qualified personnel.8ExplodedViewNoteWhen ordering replacement parts,be sure to order by part number —not by key number.M26120-33,M26120,M26125,series 11069Model Type:M26100,Series 1107Model Type:M71000,Series 1106Model Type:M26100,Series 1107Model Type:M71000,Series 1106Product Name:Speci Mix Aliquot Mixer Product Name:1/2Size Speci Mix Aliquot Mixer Key Part #(Qty)DescriptionPart #(Qty)Description*PT261X1Silicone top tube holder PT532X2Silicone top tube holder 2PT1106X4End plate assembly PT1106X4End plate assembly 3CV710X1A End coverCV710X1A End cover4PT1106X3End plate assembly PT1106X3End plate assembly 5MTX34Motor (M26125)MTX34Motor (M71015)MTX35Motor (M26120)MTX35Motor (M71010and -33)6CR1105X1Cord set (M26125)CR1105X1Cord set (M71015)CR1105X2Cord set (M26120-33)CR1105X2Cord set (M71010-33)CRM1855X1Cord set (M71010-33CN)7SWX135Switch (M26125)SWX135Switch (M71015)SWX136Switch (M26120-33)SWX136Switch (M71010-33)8PT261X3B Top rocker plate assembly PT532X1A Top rocker plate assembly 9CUX4Coupling CUX4Coupling 10LN261X1LinkLN261X1LinkReplacementPartsWarningTo avoid electrical shock,always discon-nect from power supply before mainte-nance and servicing.Refer servicing to qualified personnel.*Not shown on exploded view.800-943-2006 or 800-926-0505North America: USA/Canada +1 866 984 3766 (866-9-THERMO) Europe: Austria +43 1 801 40 0, Belgium +32 2 482 30 30, France +33 2 2803 2180, Germany national toll free 08001-536 376,Germany international +49 6184 90 6940, Italy +39 02 02 95059 434-254-375, Netherlands +31 76 571 4440, Nordic/Baltic countries +358 9 329 100,Russia/CIS +7 (812) 703 42 15, Spain/Portugal +34 93 223 09 18, Switzerland +41 44 454 12 12, UK/ Ireland +44 870 609 9203Asia: China +86 21 6865 4588 or +86 10 8419 3588, India toll free 1800 22 8374, India +91 22 6716 2200, Japan +81 45 453 9220,Other Asian countries +852 2885 4613 Countries not listed: +49 6184 90 6940 or +33 2 2803 2180。
CTS™ TrypLE™ Select Catalog Numbers A4738001 and A1285901Pub. No. MAN0007390 Rev.2.0WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. Safety Data Sheets (SDSs) are available from /support.Product descriptionTrypLE™, an animal origin-free alternative to porcine trypsin, is a recombinant enzyme derived from microbial fermentation. TrypLE™is used for the dissociation of attachment-dependent cell lines from plasticware. TrypLE™ has demonstrated ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The Gibco™ CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.Contents and storage[1]Shelf Life duration is determined from Date of Manufacture.Procedural guidelines•Formulated in DPBS with 1 mM EDTA.•Substitutes directly into existing protocols.•No inactivation required; dilution alone inactivates TrypLE™avoiding the need for trypsin inhibitors.Detach cellsTrypLE™ is designed as a direct substitute for trypsin in existing protocols. Optimal conditions and concentrations employed for individual systems should be determined empirically.1.Prewarm TrypLE™ and complete growth medium to 37°Cbefore use. Minimize dwell time.Note: TrypLE™ may be used at ambient room temperature for many types of cells.2.Aspirate spent medium and discard.3.Wash cell monolayer with 5 mL of prewarmed CTS™Dulbecco’s Phosphate Buffered Saline (DPBS) withoutcalcium and magnesium. Aspirate and discard.4.Add an appropriate volume (e.g., 5 mL in a 75 cm2flask)of TrypLE™ to the flask. Ensure complete coverage of cellmonolayer with TrypLE™.5.Incubate at 37°C until cells have detached. Observe cellmonolayer using an inverted microscope to ensure complete cell detachment from the surface of the flask. Gently tapflask to dislodge cells.6.Add 5–10 mL of complete medium to the flask. Tilt the flaskin all directions to thoroughly rinse the flask. Transfer the cell suspension to a 15‑mL conical tube.7.Centrifuge at 100 × g for 5–10 minutes.8.Discard supernatant and resuspend the cell pellet in 2–5 mLof complete medium.9.Determine the viable density using a Countess™ AutomatedCell Counter or alternative automated or manual method.10.Seed, incubate, and subculture according to normalprotocols depending on your cell type.Note: Use of soybean trypsin inhibitor is not recommended.For Research Use or Manufacturing of Cell, Gene, or Tissue- Based Products. CAUTION: Not intended for direct administration into humans or animals.Related productsLimited product warrantyLife Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale at /us/en/home/global/terms-and-conditions.html. If you have any questions, please contact Life Technologies at /support.Life Technologies Corporation | 3175 Staley Road | Grand Island, New York 14072 USAFor descriptions of symbols on product labels or product documents, go to /symbols-definition.The information in this guide is subject to change without notice.DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses.Limited Use Label License No. 517: Internal Research and Bioproduction Use: Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services conducted by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component; (3) reverse engineer the product or cause the product to be reverse engineered; or (4) use the product for purposes other than what is indicated in this Limited Use Label License. Life Technologies is not aware of Intellectual Property ("IP") that would be infringed by the manufacture or sale of its products. Customers are urged to perform an IP search and analysis specific to their manufacturing processes and target biologic products. Should that analysis require information about Life Technologies products, Life Technologies will provide to the customer's IP counsel the information necessary to analyze any relevant IP in a manner that protects Life Technologies proprietary information. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact ***************************** Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, California 92008 USA©2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified./support | /askaquestion5 March 2021。
Flexibility · Effi ciency · QualityPart of Thermo Fisher Scientifi cThermo Scientifi c MicromSuperior technique Safety EconomicTechnical data/accessoriesTechnical data STP 120Capacity Reagent stationsNumber of vessels: 10 (9 if 3 paraffi n baths are used)Volume per vessel: 1.8 l Paraffi n stations Number: 2 (optionally 3)Volume: 1.8 lNominal voltage: 24 V AC Nominal power per station: 100 VA Temperature setting range: 50 - 70°C Overtemperature release: 75°C >(± 4°C)Cassette basketsNumber of baskets: 1 (optionally 2)Basket capacity: 120 cassettes ProgrammingNumber of programs: 10 (selectable)Programmable infi ltration time per station: From 1 sec. to 99 h 59 min Spiral agitation: selectable Vertical agitation: selectable Centrifuging: selectable Centrifuging time: selectableProgram start delay selectable without time limit Standard equipment 1 Basic instrument10 Plastic vessels for reagents 2 Paraffi n baths, aluminium1 Stainless steel basket (capacity for 120 cassettes)1 Tool kit 1 User manual Optional equipment1 Spiral support for a second cassette basket (thus doubling the processing capacity up to 240 cassettes 1 Additional basketAdditional paraffi n bath (if the instrument with two cassette baskets is used, it should incorporate a third paraffi n bath)Vapor extraction system, with active charcoal fi lter OptionsSTP 120-1: Standard instrumentSTP 120-2: Standard instrument + fume extraction system with active charcoal fi lterSTP 120-3: Standard instrument + fume extraction system with active charcoal fi lter + 3rd paraffi n station and 2nd cassette basketDimensions: Diameter: 850 mm Height: 500-700 mm Diameter of the roller`s circle: 670 mm Weight: Net (fully equipped): 70 kgNominal voltage: 100-120 V / 220-240 V AC (± 10%)Network`s frequency: 50-60 HzRev. 11/08ManufacturerMicrom International GmbH Part of Thermo Fisher Scientifi c Robert-Bosch-Str. 4969190 Walldorf/Germany Phone: +49(0)6227-8360Fax: +49(0)6227-836111e-mail: info.dxd.dewal@thermofi Internet: Anatomical PathologyAccessories• Plastic vessels with handle• Stainless steel basket with capacity for 120 cassettes • Stainless steel counterweight to keep the specimens submerged • Paraffi n bath, aluminiumParaffi n Type 1, 3, 6, 9These four different blends of highly refi ned paraffi n and polymers offer a choice of infi ltration times and cutting characteristics to meet all your needs. All paraffi ns melt between 55°-57°C and are triple-fi ltered to 0.5 microns.TurbOfl ow™ CassettesTurbOfl ow cassettes are uniquely designed to hold specimens securely and ensure optimal fl uid exchange, 85% more than traditional cassettes. This helps reduce reagent carryover and when used with the TurbOfl ow base molds, will use less paraffi n and virtually eliminate fl ash.Centrifuging (Whirling)This function is activated as soon as the in-fi ltration time in a reagent is elapsed. Thebasket rises above the reagent’s level, but inside the vessel. During a period of time of 60 seconds it rotates at high speed and auto-matically changes the rotation direction every 15 seconds, which enables the basket and the specimens to be optimally drained.Via this new function it is possible for the reagents to last longer, as their contamination is reduced to a minimum since reagents are not carried over from one vessel to the next.Due to these two new functions, the quality of the specimens is equal to the quality obtained by a vacuum instrument.Ergonomic control panelThe buttons of the control panel are arranged ergonomically for easy handling.The LCD display shows all the parameters throughout the process, such as program number, vessel, remaining time, start time, start delay, total duration of the program, spiral agitation & basket’s centrifuging, temperature of paraffi n baths, date and time.Maximum safety standards for the user and specimensThe individual covers for each one of the vessels reduce the emissi-on of vapors to a minimum.The STP 120-2 and STP 120-3 versions incorporate a fume extraction system that – through a fan and an active charcoal fi lter – cleans the vapors. The tissue samples are protected against desiccation and solid paraffi ns in case of a power failure. In case of a power shutdown, the specimens are automatically lowered inside the vessel through a battery. Once power is restored, the instrument resumes the program at the same point in which it was interrupted. If it is a long power failure and the paraffi n baths become solidifi ed, the safety program will be activated. The instrument will then wait for the baths to be fully liquifi ed before going ahead with the change to the paraffi n baths.Emergency motions can be operated through the battery, such as moving the basket up and down, change it to another station. Emergency stop button. Possibility to interrupt a program for reloading and advance sampling.Insulation of the electric and eletronic components from the working areas.Correction and alarm during program deviationsIn case the specimens remain inside a station for a longer time than the programmed time, e.g. due to a power failure, the display will show a message indicating the station number and the overtime spent in that stationcompared with the initially programmed time. The acoustic and visual alarms can easily be identifi ed by the user.The keyboard can be locked by the user to avoid an inadvertentchange of the process parameters during operation.A new technology ensuring highperformance with advanced ergonomicdesignMicrom is setting new standards through innovative technical developments for tissue infiltration, through the combination of an efficient and well-tested technology with advanced design and performance.The STP 120 Tissue Processor incorporates new functions that haveenabled us to develop the most advanced Carousel Tissue Processorin the world.Spiral agitation of the cassette basketDuring the infiltration process, this function makes it possible for the basket to turn permanently and to automatically change the rotationdirection every 60 seconds. This reduces the processing times and obtains a full and homogenous mixture of the reagents plus a perfect infi ltration.FlexibilityThe combination of silent and robust precision mechanics, plus a new software, enables the user to attain the highest degree of fl exi-bility, as all functions can be programmed. Through this versatility each user can adjust the instrument to the specifi c requirements of each laboratory.Various options• STP 120-1 Basic instrument• STP 120-2 Basic instrument + fume extraction system with active charcoal fi lter• STP 120-3 Basic instrument + fume extraction system with active charcoal fi lter + 3rd paraffi n station and 2nd cassette basket.The STP 120-3 version doubles the instrument’s capacity by incorporating a second cassette basket, thus processing up to 240 cassettes and signifi cantly increasing the effi ciency of routine and research jobs.Ease of useThe instrument has capacity to store up to 10 different programs that can be freely set up by the user. Each one of the programs can be started in immediate or delayed mode, without time limit.The instrument can easily be rotated via four rollers. This allows the user to have fast and safe access to each one of the vessels.*A c c e s s o r i e s n o t i n c l u d e d .120。
NEW!Thermo Scientific Revco®PLUS -86°C FreezersProtecting Your Life’s WorkYour life’s work. What is it worth to you?Y our life’s work.What is it worth to you?Revco Ultima ®PLUS: Advanced Performance, Controls and Monitoring Revco Elite ®PLUS: High Performance and Controls Revco Value ®PLUS: Basic Functionality and Controls689You spend countless lab hours developing valuable samples. Many are irreplaceable. Protecting the integrity of your work is not just important, it’s immeasurable.For more than 50 years, Thermo Scientific Revco cold storage products have been trusted by theworld’s leading global biotechnology, pharmaceutical,academic, government, and clinical laboratories.More than two billion samples are stored in Thermo Scientific cold storage equipment worldwide – more than any other brand. Recognized for our dependability and quality, our cold storage solutions cover the broadest spectrum of temperature needs from +4°C to -196°C.Thermo ScientificRevco PLUS -86°CFreezers* based on internal performance dataIntroducing the new Thermo Scientific Revco PLUS -86°C upright freezers, combining the highest reliability and superior performance with cost-effective operation and innovative features.Leveraging years of R&D expertise, our newRevco PLUS freezers are designed with a patented refrigeration system and new robust electronics platform to deliver:Maximum Temperature Recovery20% more heat removal capacity over other leading freezers, ensuring faster temperature recovery after door openings.*Operational EfficiencyUses 15% less power to maintain cabinet tempera-ture compared to other leading freezers.* In addition,the Revco PLUS emits 15% less heat into the lab environment, minimizing air conditioning costs.*Comfortable Lab EnvironmentQuiet, comfortable operation allows our -86°C freezers to reside directly in the lab, creating amore productive and efficient work environment.Our life’s work is safeguarding your life’s work.1Our Revco PLUS -86°C upright freezers include three models, with varying levels of monitoring, reporting and alarms. Whatever controls your environment requires,we have a Revco PLUS model to meet your needs.Revco Ultima PLUS :Advanced, eye-level control platform designed for applications requiring very detailed, sophisticated monitoring,alarm and communication functions.Revco Elite PLUS :Mid-range, knee-level control platform designed for applications requiring a moderate level of monitoring, alarm and communication functions.Revco Value PLUS :Basic, knee-level control platform designed for applications requiring basic monitoring, alarm and communication functions.Advanced Technology, Exceptional QualityOur Revco PLUS freezers meet the sample storage and protection needs of different lab environments.All Revco PLUS models feature the same technologyplatform with the advanced refrigeration, microprocessor controls and high-quality construction.for quieter, comfortable lab surroundingsMinimized Noise Output Featuring advanced noiseabatement technology and insulation, our Revco PLUS -86°C freezers are 40% quieter so theycan reside right in your lab.speeds sample preparation and minimizes sample exposure toambient air.3Washable Filter:protection fromdust build-up on condenserPLUS Power Management System:low voltage surge protection, voltage Advanced Compressor Deck:superior heat removal and energy efficiency; excellent insulation for a very quiet work environmentCFC-Free Refrigerants:DuPont Suva 95 in low stage models Door-Mounted Pressure Equalization Port (PEP): maintenance-free PEP allows for quick re-entries after door openings and eliminates the need for ice removal(PEP is hidden from view)4Stainless Steel Interior Shelves:Quality-Built for Maximum ReliabilityFor optimized performance and operation, our Revco PLUS -86°Cfreezers feature robust, high-quality construction with advancedinsulation, flexible storage and precision controls.Ensure the easy retrieval of precious samples and minimize sampleexposure to ambient conditions with our Revco rack solutions.Your sales representative can suggest the optimalrack configuration for your storage needs.56Thermo Scientific Revco Ultima PLUS -86°C Upright Freezers7Revco Elite PLUS High Performanceand ControlsOur Revco Elite PLUS -86°C freezer is available in 17, 21 and 25 cubic feet capacities.Alarm Test: simulates tempera-ture alarm conditionTouchpad Data Entry:adjustable in 1°C increments Alarm Reset:mutes audible alarmand sets A.I.M.Value PLUS:Basic Performance and ControlsOur Revco Value PLUS -86°C freezer is available in 17, 21 and 25 cubic feet capacities.Touchpad Data Entry: adjustable in 1°C increments; alarm setpoint in 5°C incrementsstandard knee-level locationPower Failure:automatically activates in eventWarranty InformationUnited States and Canada:Standard Revco PLUS models carry a 2-year warranty on parts and labor and a total 3, 4, or 5 year warranty on compressor parts for the Value PLUS, Elite PLUS, and Ultima PLUS, respectively.International (Outside of U.S. and Canada):Standard Revco PLUS models carry a 16-month full warranty for parts and a total 60-month warranty on compressor parts for the Value PLUS, Elite PLUS, and Ultima PLUS.Certification information and Line Cord Info • Meets NEMA, UL61010-1, UL471, CUL, CE,TUV, IEC60335-2-89/Climate Class T (Tropical) criteria• Calibration Certification Available • Validation Testing Program AvailableThermo Scientific Revco Elite PLUS -86°C Upright FreezersLine Cord Plug ReferenceFreezers supplied with line cord plugs (P) require corresponding receptacles illustrated in the Line Cord Plug Reference. Catalog selection charts list each standard model with voltage, rated amperage,recommended circuit breaker size and NEMA electrical plug information.。
逆转录技术手册本文档中的信息如有更改，恕不另行通知。
免责声明Thermo Fisher Scienti c及其附属公司不对本文档提供任何明示或暗示的保证，包括但不限于适销性、特定用途适用性或非侵权性的保证。
在任何情况下，无论是否以合同、侵权、担保、抑或任何法规为依据，或以任何其他特殊依据为基础，对与本文档或因本文档引起的（包括但不限于对本文件的使用）特殊、偶然、间接、惩罚性、多重或后果性的损害。
在法律允许范围内，Thermo FisherScienti c及其附属公司均不承担责任。
目录逆转录 | iii仅供科学研究使用1 逆转录 (1)1.1 发现 (1)1.2 发展 (1)1.3 应用...........................................................22 建立逆转录反应 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32.1 步骤 (3)2.1.1 RNA 模板制备 (3)2.1.2 基因组DNA 去除 (6)2.1.3 选择引物 (6)2.1.4 进行逆转录 (8)2.1.5 基因组DNA 去除 (9)2.2 常见逆转录酶及性质 (9)2.2.1 DNA 聚合酶活性 (9)2.2.2 RNase H 活性 (10)2.2.3 热稳定性 (11)2.2.4 持续合成能力 (12)2.2.5 保真度 (14)2.2.6 末端转移酶（TdT ）活性 (14)2.3 常见问题及解决建议 (15)2.3.1 RT -(q)PCR 扩增量低或未扩增 (15)2.3.2 RT -(q)PCR 非特异性扩增 (16)2.3.3 cDNA 截短 (17)2.3.4 cDNA pool 覆盖率低 (18)2.3.5 cDNA 测序错误 (19)3 应用 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203.1 逆转录聚合酶链式反应（RT -PCR ）. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203.1.1 RT -PCR (20)3.1.2 定量RT -PCR (27)3.1.3 直接RT -qPCR (28)3 .2 cDNA克隆和文库构建 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293.3 cDNA末端快速扩增 (32)3.4 基因表达芯片 (34)3.5 RNA测序 (36)3.6 逆转录环介导等温扩增（RT-LAMP） (38)iv | 逆转录仅供科学研究使用1 逆转录本章内容涵盖逆转录基础、cDNA合成、酶选择、常见问题处理技巧以及cDNA在分子生物学研究中的应用，是适用于各种层级研究人员的学习资源。
No need to turn off yourmeter to switch modules –meter automatically recognizesmodule changes.This meter is thecomplete package – up tofour channels to mix andmatch with easy-to-usekey layouts and graphics.Orion VERSA STAR meters can be customized to display only the information that you need.Ordering InformationCat. No.DescriptionVSTAR00Orion VERSA STAR Benchtop Meter- Electrode arm with redesigned holder- Universal power adapterVSTAR10Orion VERSA STAR pH Benchtop Meter Set- Orion VERSA STAR meter withpH/temperature module- Electrode arm with redesigned holder- Universal power adapterVSTAR12Orion VERSA STAR pH Benchtop Meter Kit- Orion VERSA STAR meter withpH/temperature module- 8302BNUMD Orion ROSS Triode pH/ATC probe- 096019 Orion Star stirrer probe- 810199 ROSS solution kit (475 mL each of pH 4,7 and 10 buffers; storage solution; cleaning solution;and pH electrode storage bottle)- Electrode arm with redesigned holder- Universal power adapterVSTAR20Orion VERSA STAR Conductivity BenchtopMeter Set- Orion VERSA STAR meter- Orion VERSA STAR conductivity/temperature module- Electrode arm with redesigned holder- Universal power adapterVSTAR22Orion VERSA STAR Conductivity BenchtopMeter Kit- Orion VERSA STAR meter- Orion VERSA STAR conductivity/temperature module- 013005MD Orion DuraProbe conductivity cell,K=0.475- 011007 conductivity standard, 1413 µS, 5x60 mL- Electrode arm with redesigned holder- Universal power adapterVSTAR30Orion VERSA STAR RDO/Dissolved OxygenBenchtop Meter Set- Orion VERSA STAR meter- Orion VERSA STAR dissolved oxygen/temperature module- Electrode arm with redesigned holder- Universal power adapterVSTAR32Orion VERSA STAR RDO/Dissolved OxygenBenchtop Meter Kit- Orion VERSA STAR meter- Orion VERSA STAR dissolved oxygen/temperature module- 083005MD Orion polarographic DO probe- 080017 calibration sleeve for DO probe- 080513 DO probe maintenance kit- 970802 BOD funnel/stirrer- 080360 BOD adapter- Electrode arm with redesigned holder- Universal power adapterVSTAR40A Orion VERSA STAR pH/ISE Benchtop Meter Set- Orion VERSA STAR meter- Orion VERSA STAR pH/ISE/temperature module- Electrode arm with redesigned holder- Universal power adapterVSTAR40A2Orion VERSA STAR pH/ISE Benchtop Meter Kit- Orion VERSA STAR meter- Orion VERSA STAR pH/ISE/temperature module- 8102BNUMD Orion ROSS Ultra pH electrode- 096019 Orion Star stirrer probe- 927007MD ATC probe, stainless steel- ROSS solution kit (475 mL each of pH 4, 7 and10 buffers; storage solution; cleaning solution; andpH electrode storage bottle)- Electrode arm with redesigned holder- Universal power adapter VSTAR40B Orion VERSA STAR Benchtop Meter with T wopH/ISE Modules- Orion VERSA STAR meter- Two Orion VERSA STAR pH/ISE/temperature modules- Electrode arm with redesigned holder- Universal power adapterVSTAR40B2Orion VERSA STAR Benchtop Meter with T wopH/ISE Modules Kit- Orion VERSA STAR meter- Two Orion VERSA STAR pH/ISE/temperature modules- 8102BNUMD Orion ROSS Ultra pH electrode- 096019 Orion Star stirrer probe- 927007MD ATC probe, stainless steel- ROSS solution kit (475 mL each of pH 4, 7 and10 buffers; storage solution; cleaning solution; andpH electrode storage bottle)- Two electrode arms with redesigned holders- Universal power adapterVSTAR50Orion VERSA STAR pH/Conductivity BenchtopMeter Set- Orion VERSA STAR meter- Orion VERSA STAR pH/temperature module- Orion VERSA STAR conductivity/temperature module- Electrode arm with redesigned holder- Universal power adapterVSTAR52Orion VERSA STAR pH/Conductivity BenchtopMeter Kit- Orion VERSA STAR meter with pH/temperature module- Orion VERSA STAR conductivity/temperature module- 8157BNUMD Orion ROSS Ultra Triode pH/ATC probe- 013005MD Orion DuraProbe conductivity cell,K=0.475- 810199 ROSS solution kit (475 mL each of pH 4,7 and 10 buffers; storage solution; cleaning solution;and pH electrode storage bottle)- 011007 conductivity standard, 1413 µS, 5x60 mL- Electrode arm with redesigned holder- Universal power adapterVSTAR80Orion VERSA STAR pH with LogR BenchtopMeter Set- Orion VERSA STAR meter- Orion VERSA STAR pH/temperature with LogRtechnology module- Electrode arm with redesigned holder- Universal power adapterVSTAR82Orion VERSA STAR pH Benchtop Meter Kit- Orion VERSA STAR meter- Orion VERSA STAR pH/temperature with LogRtechnology module- 8172BNX Orion ROSS Sure-Flow pH electrode- 927007MD ATC probe, stainless steel- ROSS solution kit (475 mL each of pH 4, 7 and10 buffers; storage solution; cleaning solution;and pH electrode storage bottle)- 810007 ROSS fill solution, 5x60 mL- Electrode arm with redesigned holder- Universal power adapterVSTAR90Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Benchtop MultiparameterMeter Set- Orion VERSA STAR meter- Orion VERSA STAR pH/ISE/temperature module- Orion VERSA STAR conductivity/temperature module- Orion VERSA STAR dissolved oxygen/temperature module- Electrode arm with redesigned holder- Universal power adapterNorth America 166 Cummings Center Beverly, MA 01915 USA Toll Free: 1-800-225-1480 Tel: 1-978-232-6000 info.water@NetherlandsTel: (31) 033-2463887 @ IndiaTel: (91) 22-4175-8800@ ©2011 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. ROSS and the COIL tradedress are trademarks of Thermo Fisher Scientific Inc. US Patent 6,793,787.ChinaTel: (86) 21-68654588 @SingaporeTel: (65) 6778-6876@JapanTel: (81) 045-453-9175@ AustraliaTel: (613) 9757-4300infowaterau@Water Analysis Instruments/waterFor more information, contact your local Thermo Scientific products dealer or call our customer and technical service experts at 1-800-225-1480 (for the US and Canada) or visit /water .S-VERSASTAR-E-1011-RevBVSTAR91Orion VERSA STAR pH/ISE/Conductivity/RDO/ Dissolved Oxygen Benchtop Multiparameter Meter Set- Orion VERSA STAR meter- Two Orion VERSA STAR pH/ISE/temperature modules - Orion VERSA STAR conductivity/temperature module - Orion VERSA STAR dissolved oxygen/ temperature module- Two 096019 Orion Star stirrer probes- Two electrode arms with redesigned holders - Universal power adapterVSTAR92Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Benchtop Multiparameter Meter Set- Orion VERSA STAR meter- Orion VERSA STAR pH/ISE/temperature module- Orion VERSA STAR conductivity/temperature module - Orion VERSA STAR dissolved oxygen/ temperature module- 8157BNUMD Orion ROSS Ultra Triode pH/ATC probe - 013005MD Orion DuraProbe conductivity cell, K=0.475 - 083005MD Orion polarographic DO probe - 080017 calibration sleeve for DO probe - 080513 DO probe maintenance kit - 970802 BOD funnel/stirrer - 080360 BOD adapter- 810199 ROSS solution kit (475 mL each of pH 4, 7 and 10 buffers; storage solution; cleaning solution; and pH electrode storage bottle)- 011007 conductivity standard, 1413 µS, 5x60 mL - Electrode arm with redesigned holder - Universal power adapterVSTAR93Orion VERSA STAR pH/ISE/Conductivity/RDO/ Dissolved Oxygen Ultimate Benchtop Multiparameter Meter Set - Orion VERSA STAR meter- Orion VERSA STAR pH/ISE/temperature module- Orion VERSA STAR conductivity/temperature module - Orion VERSA STAR dissolved oxygen/ temperature module- 8157BNUMD Orion ROSS Ultra Triode pH/ATC probe - 013005MD Orion DuraProbe conductivity cell, K=0.475 - 083005MD Orion polarographic DO probe - Two 096019 Orion Star stirrer probes - 080017 calibration sleeve for DO probe - 080513 DO probe maintenance kit - 970802 BOD funnel/stirrer - 080360 BOD adapter- 810199 ROSS solution kit (475 mL each of pH 4, 7 and 10 buffers; storage solution; cleaning solution; and pH electrode storage bottle)- 011007 conductivity standard, 1413 µS, 5x60 mL - Two electrode arms with redesigned holders - Universal power adapterAccessories VSTAR-PH Orion VERSA STAR pH/temperature module VSTAR-ISE Orion VERSA STAR pH/ISE/temperature module VSTAR-CND Orion VERSA STAR conductivity/temperature module VSTAR-RD Orion VERSA STAR RDO/DO/temperature module VSTAR-LROrion VERSA STAR pH/temperature with LogR technology moduleSTARA-BEA Benchtop electrode arm for Orion Star A-series meters STARA-HB Freestanding base for use with Orion Star A-seriesbenchtop electrode arm STARA-PWR Replacement universal power adapter for Orion VERSA STAR meters096019Orion Star stirrer probe8102BNUWP Orion ROSS Ultra pH electrode, refillable, glass body 8172BNWP Orion ROSS Sure-Flow pH electrode, glass body 8156BNUWP Orion ROSS Ultra refillable pH electrode, epoxy body 8157BNUMDOrion ROSS Triode pH/ATC electrode, refillable, epoxy body 8302BNUMD Orion ROSS Triode pH/ATC electrode, refillable,glass body8172BNXOrion ROSS Sure-Flow pH electrode, glass body 8611BNWP Orion ROSS sodium combination ISE 927007MD Orion ATC probe, stainless steel body 927005MD Orion ATC probe, epoxy body013005MD Orion DuraProbe conductivity cell, 4-cell, K=0.475013016MD Orion conductivity cell, flow through, K=0.1083005MD Orion dissolved oxygen probe, polarographic 086030MD Orion auto-stir DO/BOD probe, polarographic 087010MD Orion RDO optical DO probe, 3 m cable810199Orion ROSS all-in-one pH buffer kit 910104Orion pH 4.01 buffer, 475 mL 910107Orion pH 7.00 buffer, 475 mL 910110Orion pH 4.01 buffer, 475 mL 011008Orion conductivity standard, 100 µS/cm, 5x60 mL bottles 011007Orion conductivity standard, 1413 µS/cm, 5x60 mL bottles 011006Orion conductivity standard, 12.9 mS/cm, 5x60 mL bottles 1010001Orion conductivity calibration resistor kit 9512HPBNWP Orion high-performance ammonia ISE 9512BNWP Orion standard ammonia ISE951007Ammonia standard, 1000 ppm, 475 mL 951210Low-level ammonia ISA 951211Ammonia ISA, 475 mL 951209Fill solution for Orion high-performance ammonia ISE, 60 mL 951202Fill solution for standard ammonia ISE , 60 mL 951213Ammonia ISE storage solution 9609BNWP Orion fluoride combination ISE040906Fluoride standard, 1 ppm with TISAB II, 475 mL 040907Fluoride standard, 2 ppm with TISAB II, 475 mL 040908Fluoride standard, 10 ppm with TISAB II, 475 mL 940909TISAB II, 1 gallon 8611BNWP Orion ROSS sodium combination ISE。
