预染蛋白marker说明书
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彩虹130广谱蛋白marker说明书货号:PR1950规格:20T(100μL)/50T(250μL)/100T(250μL×2)/500T(250μL×10)保存:-20℃保存,有效期至少2年。
产品特点:●三色预染,颜色鲜亮,条带整齐,便于观察。
●用量少,节约成本,仅5ul即可完美呈现(1.5mm,10孔梳)。
●广谱多带,一支即可满足多种实验需求。
产品简介:本产品包含9种彩色预染的已知分子量标准蛋白,分子量范围为15kD-130kD,每种蛋白含量约为0.2-0.4mg/ml。
预染marker可以用于直接观察蛋白质电泳状况以及清晰地判断Western Blot的转膜效果。
经SDS-PAGE凝胶电泳或转移到PVDF或NC膜上可得到清晰的9条彩色蛋白条带,其中70kD条带为红色,25kD条带为绿色,其余条带为蓝色。
使用说明:1.本产品是即用型液体,可直接上样电泳。
上样前无需加热,稀释或添加还原剂。
2.上样5ul,SDS-PAGE电泳过程及转膜后可看见清晰的彩虹条带。
3.建议分离胶浓度为15%。
电泳示意图说明:第1页,共2页第2页,共2页注意事项:1.本产品含有较高浓度甘油,常规-20℃保存为液体状态,可直接使用。
若冰箱温度不稳,导致结冰,可适当分装后置于4℃保存,至少稳定6个月。
2.Marker分离效果与PAGE胶浓度相关,若分离胶浓度低于15%,25kD以下条带不易分离,但不影响绿色(25KD)及以上条带。
如果目的条带小于25KD,建议分离胶浓度大于或等于15%。
3.转膜效果与转膜时间有关,需根据客户目的条带大小而定,如果转膜后较大分子量条带有部分未转膜成功,属于正常现象。
Livning彩色预染蛋白marker(10-180KD)with45kDa使用说明书产品名称规格货号Livning彩色预染蛋白marker(10-180KD)with45kDa250μl*1LVN150-1Livning彩色预染蛋白marker(10-180KD)with45kDa250μl*2LVN150-2Livning彩色预染蛋白marker(10-180KD)with45kDa2x250μl*10LVN150-10【储存条件】-20˚C恒温长期保存,4˚C保存6个月,建议分装保存,避免反复冻融。
【产品简介】本产品由跨度从10~180kDa的10种纯化的天然蛋白混合而成,各条带浓度约为0.2~0.4mg/ml。
其中75kDa条带为红色预染条带,方便判断各个条带的准确位置。
本产品适合作为SDS-PAGE电泳时,变性蛋白样品的分子量参照,并可实时观察蛋白样品的电泳分离状况,也可用于检测Western blot的转膜效率。
由于共价结合的染料会影响蛋白质分子的电泳迁移率,本产品适于粗略地估计目的蛋白样品的分子量。
【使用方法】1.将本产品于室温融化后,轻柔混匀,使沉淀充分溶解;2.加入3~5μl到SDS-聚丙烯酰胺胶的上样孔中,与待测样品一起电泳和转膜;3.电泳结束后,通过考马斯亮蓝染液染色观察条带。
【注意事项】1.使用时应该将从冰箱中取出的产品恢复至室温后使用,否则可能由于低温下蛋白变性不彻底导致电泳条带出现不同程度的弥散;2.使用前先将产品恢复至室温后混匀,使沉淀充分溶解,否则可能导致电泳条带出现不同程度的弥散或拖带;3.本产品含有SDS,蛋白已变性,不宜作为天然蛋白分子电泳时的分子量参照标准。
【图列展示】(单一浓度PAGE胶,Tris-Glycine电泳缓冲液,实际电泳图)8686************(梯度胶和单一浓度胶,Tris-Glycine 电泳缓冲液,带型示意图)【附录:转膜和洗膜】A 、转膜条件(冰上进行):a.Transfer with buffer containing 20%methanol to fix proteins on membrane.b.Wash membrane with PBS or TBS containing less than 0.1%Tween-20at 4°C.B 、洗膜条件(4度进行):Membrane:Nitrocellulose membranes /PVDFWash Buffer:1X Tris buffered saline,0.1%Tween-20(TBST)(吐温20不能超过0.1%)C 、Stripping Buffer:15g Glycine,1g SDS,10ml Tween20,pH2.2–Adjust volume to 1L如果需要用到含有DTT /b-ME 的Stripping buffer ,膜需要先以1X Tris buffered saline(TBS)洗三次,把Tween-20去干净后,再进行Stripping 。
SM0671PageRuler Prestained Protein LadderA prestained SDS-PAGE MW marker with contrasting colored reference bands at10 and 70kDa.The Thermo Scientific PageRuler Prestained Proteis a mixture of ten (10) blue-, orange- and green-srecombinant proteins (10 to 170kDa) for use as sizstandards in protein electrophoresis (SDS-PAGE)Western blotting.This prestained protein MW marker is designed fomonitoring the progress of SDS-polyacrylamide geelectrophoresis, for assessing transfer efficiency onylon and nitrocellulose membranes, and for estimapproximate size of separated proteins that have b visible with gel stains or Western blot detection reagents. The ladder contains one orange refer at 70kDa and one green band at 10kDa.Highlights:•Size range– 10 proteins spanning 10 to 170kDa•Ready-to-use– supplied in a loading buffer for direct loading on gels; no need to boil •Sharp bands– color-coded bands of similar intensity for easy visualization•Quality tested – each lot evaluated by SDS-PAGE and Western blotting•Two reference bands– orange at 70kDa and green at 10kDa•Membrane-compatible– colored bands transfer to membranes for Western blottingIncludes:•Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 1 1mM NaN3 and 33% glycerol.Applications:•Monitoring protein migration during SDS-polyacrylamide gel electrophoresis•Monitoring protein transfer onto membranes after Western blotting•Sizing of proteins on SDS-polyacrylamide gels and Western blotsProduct Details:SDS-PAGE band profile of the Thermo ScientificPageRuler Prestained Protein Ladder. Images arefrom a 4-20% Tris-glycine gel (SDS-PAGE) andsubsequent transfer to membrane.Sm1841Spectra Multicolor Broad Range Protein LadderA prestained, 4-color, SDS-PAGE MW marker especially for proteins between 10 and 260kDa.The Thermo Scientific Spectra Multicolor Broad RangeProtein Ladder is a 4-color protein standard containing 10prestained recombinant prokaryotic proteins (10 to 260kDa)for use as size standards in gel electrophoresis and Westernblotting.This prestained protein MW marker is designed formonitoring the progress of SDS-polyacrylamide gelelectrophoresis, for assessing transfer efficiency onto PVDF,nylon and nitrocellulose membranes, and for estimating theapproximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Four different chromophores (blue, orange, green, pink) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern.Highlights:•Size range– 10 proteins spanning 10 to 260kDa•Multicolor– four different colors for unambiguous band-size assignment•Ready-to-use– supplied in a loading buffer for direct loading on gels; no need to boil•Sharp bands– color-coded bands of similar intensity for easy visualization•Quality tested – each lot evaluated by SDS-PAGE and Western blotting•Membrane-compatible– colored bands transfer to membranes for Western blotting Includes:•Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.Applications:•Monitoring protein migration during SDS-polyacrylamide gel electrophoresis•Monitoring protein transfer onto membranes after Western blotting•Sizing of proteins on SDS-polyacrylamide gels and Western blotsProduct Details:SDS-PAGE band profile of the Thermo ScientificSpectra Multicolor Broad Range Protein Ladder.Images are from a 4-20% Tris-glycine gel(SDS-PAGE) and subsequent transfer to membrane.S m1811PageRuler Plus Prestained Protein LadderA prestained SDS-PAGE MW marker with contrasting colored reference bands at 10, 25 and 70kDa.The Thermo Scientific PageRuler Plus Prestained ProteinLadder is a mixture of nine (9) blue-, orange- andgreen-stained recombinant proteins (10 to 250kDa) for useas size standards in protein electrophoresis (SDS-PAGE)and Western blotting.This prestained protein MW marker is designed formonitoring the progress of SDS-polyacrylamide gelelectrophoresis, for assessing transfer efficiency onto PVDF,nylon and nitrocellulose membranes, and for estimating theapproximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel.Highlights:•Size range– nine proteins spanning 10 to 250kDa•Ready-to-use– supplied in a loading buffer for direct loading on gels; no need to boil•Sharp bands– color-coded bands of similar intesity for easy visualization•Quality tested – each lot evaluated by SDS-PAGE and Western blotting•Bright reference bands– orange at 70 and 25kDa, and green at 10kDa•Membrane-compatible– colored bands transfer to membranes for Western blotting Includes:•Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.Applications:•Monitoring protein migration during SDS-polyacrylamide gel electrophoresis•Monitoring protein transfer onto membranes after Western blotting•Sizing of proteins on SDS-polyacrylamide gels and Western blotsProduct Details:SDS-PAGE band profile of the Thermo Scientific PageRuler Plus Prestained Protein Ladder. Images are from a 4-20% Tris-glycine gel(SDS-PAGE) and subsequent transfer to membrane.。
CERTIFICATE OF ANALYSISPageRuler ™Prestained Protein Ladder#SM067210 x 250 µl(for 100 mini gel applications 5 µl per well or 50 large gel applications 10 µl per well)Lot: Expiry Date:Storage: stable at 4°C for up to 3 months. For long term storage, store at -20°C.SM067_57_9.docDescriptionPageRuler ™Prestained Protein Ladder is a mixture of 10 recombinant, highly purified colored proteins with apparent molecular weights of 10 kDa to 170 kDa. Ladder proteins are covalently coupled with a blue dye except for two reference bands prestained with different colors. The 72 kDa reference band is orange and 10 kDa reference band is green.The ladder is supplied in gel loading buffer and isready-to-use: no heating, further dilution or addition of a reducing agent is required.ContentsApproximately 0.1-0.2 mg/ml of each protein in the storage buffer (62.5 mM Tris-H 3PO 4 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 10 mM DTT, 1 mM NaN 3 and 33% (v/v) glycerol).Applications∙ Monitoring of protein separation during SDS-PAGE (1). ∙ Verifying Western transfer efficiency (2, 3).∙ Approximate sizing of proteins on SDS-polyacrylamidegels and Western blots.Instruction for Use❶ Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. DO NOT BOIL!❷ Mix gently, but thoroughly, to ensure the solution is homogeneous.❸ Load the following volumes of the ladder on an SDS-polyacrylamide gel:– 5 µl per well for mini gel,– 10 µl per well for large gel.Use the same volumes for Western blotting.❹ After the run is complete, stain the gel or perform Western transfer procedure as desired.Note•Each lot of the PageRuler™ Prestained Protein Ladder is calibrated against a precisely sized, PageRuler™ Unstained Protein Ladder and calculated apparent molecular weights are reported in the picture.•For precise molecular weight determinations use PageRuler™ Unstained Protein Ladder, #SM0661, see.•In 8 or 10% gels low molecular weight proteins may migrate with the dye front.•Loading volumes are intended for use in gels with a thickness of 0.75 mm. For thicker gels, the recommended loading volume should be increased.•PageRuler™ Prestained Protein Ladder could be used in Western blotting with all common membranes: PVDF, nylon and nitrocellulose.•Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100 kDa) proteins.Lot specific MW, kDa4-20% Tris-glycine SDS-PAGEcontinued on back pageQUALITY CONTROL5 µl of PageRuler™ Prestained Protein Ladder resolves 10 bands of equal intensities in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting onto PVDF membrane.Quality authorized by: Jurgita Zilinskiene References1. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685, 1970.2. Burnette, W.N., "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate – polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A, Anal. Biochem., 112 (2), 195-203, 1981.3. Towbin, H., et al., E lectrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350-4354, 1979.This product is manufactured under the license forStrep-tag® technology covered by US patents Nos.5,506,121, 6,103,493 and foreign counterparts. Related Products∙DualColor™ Protein Loading Buffer Pack #R1011∙Loading Buffer Pack #R0891∙Spectra™ Multicolor Broad Range Protein Ladder #SM1841∙PageRuler™ Unstained #SM0661∙PageRuler™ Plus Prestained Protein Ladder #SM1811∙PageSilver™ Silver Staining Kit #K0681∙PageBlue™ Protein Staining Solution #R0571∙10X Tris-glycine-SDS Buffer #B46∙10X Tris-tricine-SDS Buffer #B48∙DTT #R0861 ∙ProteoJET™ Mammalian Cell Lysis Reagent #K0301∙ProteoJET™ Cytoplasmic and Nuclear ProteinExtraction Kit #K0311∙Bradford Reagent, ready-to-use #R1271∙Bovine Serum Albumin Standard Set, ready-to-use #R1281∙Bovine Gamma Globulin Standard Set, ready-to-use #R1291PRODUCT USE LIMITATION.This product is developed, designed and sold exclusively for research purposes andin vitro use only. The product was not tested for use in diagnostics or for drugdevelopment, nor is it suitable for administration to humans or animals.Please refer to for Material Safety Data Sheet of the product.。
蛋白marker标记颜色方法全文共四篇示例,供读者参考第一篇示例:蛋白marker标记颜色方法在生物科学研究中,标记蛋白是非常常见的实验技术之一。
蛋白marker的标记可以帮助研究人员在实验中追踪和定位蛋白,从而更好地理解蛋白的功能和相互作用。
而标记蛋白的颜色选择也是非常重要的,不同颜色的标记能够帮助实验者在实验过程中更清晰地识别和分析样品。
本文将介绍一些常用的蛋白marker标记颜色方法,希望能够帮助读者更好地进行蛋白标记实验。
一、荧光标记荧光标记是蛋白标记颜色中最常用的方法之一。
通过将蛋白与荧光物质结合,可以在光学显微镜下直接观察到蛋白标记的颜色。
常用的荧光标记包括FITC(荧光异硫氰酸酯),TRITC(罗丹明异硫氰酸酯)和Cy5(Cyanine5)。
这些荧光物质在实验中常用于免疫荧光染色、融合蛋白标记等实验中。
荧光标记的优势是信号强度高、灵敏度高且不受环境影响,适用于单细胞和组织的标记。
在实验中,荧光标记的颜色可以根据荧光物质的不同而有所区分。
比如,FITC标记的蛋白呈现绿色,TRITC标记呈现橙红色,Cy5标记呈现红色。
实验者可以根据实验需要选择不同的荧光标记物质,并通过显微镜观察到标记蛋白的颜色。
二、生物素标记生物素标记是一种基于生物素-亲和素相互作用的蛋白标记方法。
生物素是一种维生素H成分,具有与亲和素结合的特性。
在实验中,生物素可以与生物素素结合并形成稳定的结合物,从而实现蛋白的标记。
生物素标记的颜色在实验中常表现为紫色,实验者可以通过观察颜色变化来判断蛋白是否标记成功。
生物素标记的优势是其结合力强、稳定性高,适用于长时间的实验操作。
另外,生物素标记也可以用于有机溶剂、高温、酸碱环境等多种条件下的蛋白标记。
因此,在一些特殊实验条件下,生物素标记是一种非常理想的标记方法。
三、酶标记酶标记是另一种常用的蛋白标记方法。
通过将蛋白与酶结合,可以在实验中通过酶的催化作用来识别蛋白的存在。
常用的酶标记包括辣根过氧化物酶(HRP)、碱性磷酸酶(AP)等。
预染超低分子量蛋白质Marker(3.3kD-31.0kD)说明书货号:PR1900规格:20T(200μ1)保存:-20℃保存,有效期为一年。
避免反复冻融,建议分装冻存。
产品简介:本产品包含预染的3种多肽和2种低分子量蛋白质组成,分子量范围为3.3kD-31.0kD。
可以用于直接观察蛋白质电泳状况以及清晰地判断Western Blot的转膜效果。
经Tricine-甘油SDS-PAGE凝胶电泳时以及转移到PVDF或NC膜上可看到清晰的5条蓝色的蛋白条带。
本品为蛋白质和多肽混合物的冻干粉,每种预染蛋白含量约为40μg,配有一支1×上样缓冲液。
使用说明:1.开封后,溶于200μ11×上样缓冲液,可根据需要进行小量分装,每管10μl,-20℃贮存,每次取一管使用,避免反复冻融。
2.使用前取分装后的小管室温融化后即可上样电泳。
电泳示意图:凝胶的配置方法:分离胶夹层胶浓缩胶20%/4.5ml16.5%/4.5ml15.5%/4.5ml10%/2ml4%/2ml49.5%T3%C///0.407ml0.160ml49.5%T6%C1.82ml1.50ml 1.395ml//凝胶缓冲液 1.50ml 1.50ml 1.50ml0.667ml0.496ml 甘油0.48ml0.48ml0.48ml//ddH2O0.70ml1.02ml1.125ml0.926ml1.344ml 10%PAGE胶凝固剂40µl40µl40µl20µl20µlPAGE胶促凝剂5µl5µl5µl3µl3µl凝胶制备及染色注意事项:1.先配制分离胶,聚合后再配制夹层胶,最后配制浓缩胶,3种胶的制胶体积比为4:1.5:1。
电泳时,30V 跑1-2h后,待指示前沿到达分离胶上沿时,把电压调至100V,至电泳结束,整个电泳过程大约需要6-8h。
Western Blot Marker说明书货号:PR1800规格:10T(50μL)/20T(100μL)保存:-20℃可保存2年。
避免反复冻融,建议分装保存。
产品简介:传统的Western Blot试验需要使用预染蛋白Marker来跟踪检测阳性抗原信号和转膜效率,但预染蛋白Marker无法在胶片上曝光,曝光信号需要根据膜上的预染蛋白Marker来判断。
Western Blot Marker是专门为Western Blot试验设计的分子量标准,由9种发光蛋白和9种预染蛋白组成;9种发光蛋白分别为23、30、36、44、50、62、90、120和160KDa,这些蛋白均可与抗体结合,因此能与抗原在同一张膜上曝出信号,阳性信号可以直接通过Western Marker的位置来判断;9种预染蛋白分别为15、25、35、40、55、70、100、130和180KDa,其中70KDa为红色,其他为蓝色,转膜完毕后在膜上肉眼可见。
主要特征:1.可与抗原同时检测信号,使Western blot结果判断更加简便2.发光Marker没有偶联和标记其他分子,分子量更加准确3.综合发光Marker和预染Marker的优势,既能监测转膜又能与抗原同时检测使用说明:1.取出后于室温放置融化;2.温和地充分混匀,取5-10μL该Western Blot Marker至上样孔中,进行SDS-PAGE电泳。
3.电泳完毕后转至PVDF膜或NC膜,与一抗二抗孵育。
4.孵育完毕,将Western Blot Marker与抗原一起通过ECL曝光至胶片或荧光显色。
图示:1、HRP标记的Goat anti mouse IgG二抗(一抗:mouse anti p38)ECL化学发光检测第1页,共2页第2页,共2页2、多重荧光检测注意事项:1.本产品可直接使用,不需要加热。
2.可根据抗体的效价或曝光时间的长短调整Western Blot Marke的上样量。
PRODUCT INFORMATIONThermo ScientificPageRuler Prestained Protein Ladder #26616 2 x 250 µlLot: XXXXXXXX Expiry Date: __WARNING! HARMFUL IF INHALED OR ABSORBEDTHROUGH SKIN. CAUSES RESPIRATORY TRACT, EYEAND SKIN IRRITATION. MAY CAUSE ALLERGIC SKIN REACTION. MAY BE HARMFUL IF SWALLOWED.CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: KIDNEYS, LUNGS, UPPER RESPIRATORY TRACT, SKIN, EYES. CONTAINS MATERIALWHICH MAY CAUSE DAMAGE TO THE FOLLOWINGORGANS: LIVER, GASTROINTESTINAL TRACT.Avoid exposure - obtain special instructions before use. Donot breathe vapor or mist. Do not ingest. Do not get in eyesor on skin or clothing. Use only with adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash thoroughly after handling. Refer to MSDS.|2661®~26616|72559?~XXXXXXXXStore at -20°C/onebio /pierce Made in Lithuania IntroductionThe Thermo Scientific™ PageRuler™ Prestained Protein Ladder is a prestained mixture of ten recombinant proteins ranging from 10 kDa to 170 kDa. Three different chromophores are bound to the proteins, producing a brightly colored ladder. The protein ladder is conveniently packaged and ready to use with no heating, diluting or additional reducing agent necessary.Lot-to-lot variation of the apparent molecular weight of prestained proteins is ~5%.Storage Buffer: 62.5mM Tris•H3PO4 (pH 7.5 at 25°C),1mM EDTA, 2% (w/v) SDS, 10mM DTT, 1mM NaN3,33% (v/v) glycerol.Important Product Information•Do not boil the protein ladder.•The protein ladder can be stored at 4ºC for up to 3 month. •For precise protein MW determination uses the PageRuler Broad Range Unstained Protein Ladder (#26630). •Each lot of the PageRuler Prestained Protein Ladder is calibrated against PageRuler Unstained Protein Ladder and calculated apparent molecular weights are reported in the lot specific product information sheet provided with the product.Rev.5Migration Patterns of PageRuler Prestained Protein LadderRecommendations for Loading1. Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil!2. Mix gently, but thoroughly, to ensure the solution is homogeneous.3. Load the following volumes of the ladder on anSDS-polyacrylamide gel:– 5 µl per well for mini gel,– 10 µl per well for large gel.Use the same volumes for Western blotting.The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading volume should be doubled for 1.5 mm thick gels.Important Notes•Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. See the table provided for migration patterns in different electrophoresis conditions.•In low-percentage gels (< 10%), the low-molecular weight proteins in the ladder may migrate with the dye front. •PageRuler Prestained Protein Ladder can be used in Western blotting with all common membranes: PVDF, nylon and nitrocellulose.•Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100 kDa) proteins.Representative picture of PageRuler Prestained ProteinLadder4-20% Tris-glycine SDS-PAGEGeneral ReferencesBurnette, W.N. (1981). “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate – polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112(2):195-203.Kurien, B.T. and Scofield, R.H. (2003). Protein blotting: a review. J Imm Meth 274:1-15.Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-5. Towbin, H., et al. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4.This product is manufactured under the license for Strep-tag® technology covered by US patents Nos. 5,506,121, 6,103,493 and foreign counterparts.PRODUCT USE LIMITATIONThis product is developed, designed and sold exclusively for research purposes and in vitro use only. The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.Please refer to /onebio for Material Safety Data Sheet of the product.© 2013 Thermo Fisher Scientific, Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific, Inc. and its subsidiaries.。