Hitachi Review Vol58 (2009), No1
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治疗性结核D N A疫苗S D S含量检测孔雯雯\魏荣华1,王玮\周飞燕1,梁艳2,吴雪琼2,倪世明、黄明\丨.广州白云山拜迪生物医药有限公司,广东广州511495;2.中国人民解放军总医院第八医学中心结核病重点实验室,北京100091)摘要:目的应用吖啶橙法来检测治疗性结核D N A疫苗中的十二烷基硫酸钠(sodium dodecyl sulfate,S D S)的残留量方法将S D S标准液与吖啶橙混合后,经甲苯萃取处理后在波长499 m n测定吸光度值,根据标准曲线计算供试品中S D S的含量 结果S D S浓度在0.005~0.32 mg.mL—1的范围内,相关系数;■ >0.99,线性关系良好…该法受高溫、辅料和核酸的影响较小3批制剂的S D S残留量均小于0.02 mg.mL—1。
结论建立的方法操作简便、专属性、准确度和重现性较好,可用于结核D N A 疫苗制剂中S D S残留量的检测,:关键词:十二烷基硫酸钠;结核;D N A疫苗;吖啶橙中图分类号:R917 文献标志码:A 文章编号:1674-229X(2021) 01-0039-04Doi :10.12048/j.issn.1674-229X.2021.01.008Determination of the Contents of Sodium Dodecyl Sulfate in Therapeutic Tuberculosis DNA VaccineK O N G Wenwen'.WEI Ronghua1,W ANG Wei1,ZH O U Feiyan1,LIA NG Yan2,W U Xueqiong2,NI Shiming1, HUANG M i n g1 ( 1. Guang"z/i〇u Bai Yun Shan Baidi Bio-Technology C o., L id, Guangzhou, Guangdong 5W495, C hina;2. Tuberculosis Key Laboratory , the Sth Medical Center o f PLA General Hospital, Beijing100091 , C hina)A B S T R A C T:O B JE C T IV E T(> develop a metlunl f or the content determination of sodiumtuberculosis DNA vaccine by acridine orange. M E T H O D S The standard solution of SDS was mixed with acridine orange, then the mixture was extrac ted with toluene and determined the absorbance at the wavelength of 499 nm.The concentration of SDS in samples could be determined by standard t;urve. R E SU L T S The standard curve of SDS concentration showed good linearity at the range of 0.005-0.32mg-niL 1 (r>0.99). And the method was relatively smaller affection by high tem perature,excipients and nucleic acid.The content of SDS from three batches of preparations were all lower than 0.02 mg • mL 1. C O N C LU SIO N The developed method is sim ple,spec ific,accurate,and reproducible,which can he used for the determination of SDS residue in tuberculosis DNA vaccine.K EY W O R D S:sodium dodecyl sulfate;tuberculosis; DNA vaccine;acridine orange结核病是由结核分枝杆菌感染引起的传染病,我国是结核病发病率较高的国家之一 目前临床使用最广泛的结核疫苗是卡介苗,它也是唯一应 用于人类结核病的预防性疫苗[4<。
NetApp存储设备配置说明修改记录目录1编写目的 (1)2专业名词和缩略语 (2)3组网方式和环境介绍 (3)4安装配置方法 (3)4.1N ET A PP硬件安装 (3)4.2设备初始化和系统设定 (4)4.2.1设备初始化 (4)4.2.2系统设定 (4)4.3操作系统安装 (6)4.3.1注册现有系统的cifs服务,将操作系统文件上传至FAS存储系统 (6)4.4应用配置 (8)4.4.1系统参数配置 (8)4.4.2注册需要使用的服务 (10)4.4.3创建一个卷并输出空间 (12)4.4.4创建一个Qtree并实施quota限制 (17)4.4.5配置autosupport (19)4.4.6配置snapshot策略及数据恢复方法 (19)4.4.7磁盘故障的数据恢复方法 (19)4.4.8配置Cluster (19)1 编写目的编写本文档的目的在于详细地说明NetApp FAS存储系统的安装、配置以及常用命令的介绍和可靠性维护、故障检查与恢复的方法,便于开发、测试、用服和工程维护人员安装、使用和维护NetApp FAS存储系统存储系统。
2 专业名词和缩略语3 组网方式和环境介绍NetAppFAS3240AESX ServerSWITCHESX Server图3.1 NetApp FAS存储系统组网结构NetApp FAS存储系统存储设备以NAS存储方式使用,通过万兆交换机与主机相连接。
4 安装配置方法4.1 NetApp硬件安装存储设备硬件的安装主要是各盘柜间线缆的连接、磁盘安装、盘柜上架、上电等,以上操作多由NetApp技术支持工程师完成。
使用存储设备随机携带的“DB-9 to RJ-45”转接线将FAS存储系统的CONSOLE端口和安装了WINDOWS操作系统的主机串口相连,在WINDOWS主机上安装SecureCRT软件,新建一个serial协议的连接,其中port参数根据所连接的是COM1还是COM2来进行选择,其余参数参考图4.1所示,通过串口连接登录到FAS存储系统。
欢迎关注本刊公众号《肿瘤影像学》2021年第30卷第2期Oncoradiology 2021 Vol.30 No.292·论 著·乳腺假血管瘤样间质增生的超声表现分析苏岳霖,王 涌复旦大学附属华山医院超声科,上海 200040[摘要] 目的:探讨乳腺假血管瘤样间质增生(pseudoangiomatous stromal hyperplasia of the breast ,PASH )的超声特征。
方法:收集2012—2019年于复旦大学附属华山医院行乳腺手术并经病理学检查诊断为PASH 的患者共10例。
回顾并分析其临床信息及超声图像表现特点。
结果:患者年龄21~59岁(平均年龄40岁),其中绝经后1例,10例均未摄入激素类药物。
9例患者超声图像表现为平行位生长肿块,1例为无肿块型,仅表现为局部回声减低伴点状钙化灶,依据乳腺影像报告和数据系统(Breast Imaging Reporting and Data System ,BI-RADS )分类标准分为0类。
9例肿块型中7例均表现为边界清晰,椭圆形,低回声,部分内部回声欠均匀(1例内部见小条状无回声区),BI-RADS 3类;1例表现边界清晰,形态欠规则,伴可疑钙化,BI-RADS 4a 类;1例表现为边界清晰,椭圆形,以实性为主囊实混合性病灶,BI-RADS 4b 类。
结论:PASH 超声图像缺乏特异性,易与纤维腺瘤相混淆,临床上应注意鉴别诊断。
[关键词] 乳腺癌;假血管瘤样间质增生;超声;病理学DOI: 10.19732/ki.2096-6210.2021.02.006中图分类号:R737.9;R445.1 文献标志码:A 文章编号:2096-6210(2021)02-0092-04Ultrasonographic diagnosis of pseudoangiomatous stromal hyperplasia of the breast SU Yuelin, WANG Yong (Department of Ultrasound, Huashan Hospital, Fudan University, Shanghai 200040, China)Correspondence to:WANG Yong E-mail:************[Abstract ]Objective: To investigate the ultrasound features of pseudoangiomatous stromal hyperplasia of the breast (PASH). Methods: A retrospective review of 10 female patients with a final pathological diagnosis of PASH, diagnosed from 2012 to 2019 at Huashan Hospital of Fudan University, was undertaken. The clinical features and ultrasound features were retrospectively reviewed. Results: The age range for 10 patients ultimately enrolled in the study was 21-59 years old, with a mean age of 40 years. One patient evaluated were menopausal, and all of them did not take hormone drugs. Nine cases of PASH showed parallel orientation mass, one case of non-mass forming, but depicted as only low echoic lesions and calcifications, Breast Imaging Reporting and Data System (BI-RADS) 0. Seven cases of 9 mass PASH showed the presence of a well-circumscribed mass, oval, with hypoechoic (1 case inner have small cysts), BI-RADS 3. One case was irregular in shape, with suspicious calcification ,BI-RADS 4a. One case was cystic-solid mixed masse, BI-RADS 4b. Conclusion: The ultrasound features of PASH are non-specific and easily confused with fibroadenoma. It should be differentiated from fibroadenoma clinically.[Key words ] Breast cancer; Pseudoangiomatous stromal hyperplasia; Ultrasound; Pathology通信作者:王 涌 E-mail:************ 乳腺假血管瘤样间质增生(pseudoangiomatous stromal hyperplasia of the breast ,PASH )是一种良性的肌成纤维细胞增生性病变,镜下表现为裂隙状假血管腔。
日立7600-020电解质模块测定结果的比对李光富;逯心敏;胡孝彬;胡容【期刊名称】《四川医学》【年(卷),期】2009(30)8【摘要】目的对日立7600-020电解质模块测定结果和Vitros250电解质测定结果进行比时.方法先检测两台仪器的精密度,再以日立7600-020测定结果作为比较方法的测定值(Y),以Vitros250电解质测定结果作为参照方法的测定值(X),对结果作直线回归分析.结果日立7600-020和Vitroa250均有良好的精密度,前者批内精密度(钾CV=0.29%,钠CV=0.36%,氯CV=0.31%).日间精密度(钾CV=0.50%,钠CV=0.46%,氯CV=0.54%);后者批内精密度(钾CV=0.26%,钠CV=0.33%,氯CV=0.29%),日间精密度(钾CV=0.48%,钠CV=0.38%,氯CV=0.50%).相关性(钾Y=0.988X+0.089,r=0.9989;钠Y=0.984X+1.138,r=0.9981;氯Y=0.980X+2.338,r=0.9986).结论两台仪器检测的结果一致,具有很好的可比性.【总页数】2页(P1318-1319)【作者】李光富;逯心敏;胡孝彬;胡容【作者单位】宜宾市第二人民医院检验科,四川,宜宾,644000;宜宾市第二人民医院检验科,四川,宜宾,644000;宜宾市第二人民医院检验科,四川,宜宾,644000;宜宾市第二人民医院检验科,四川,宜宾,644000【正文语种】中文【中图分类】R446.1【相关文献】1.全自动生化分析仪日立7600-020与日立7080比对分析 [J], 张利;杨正芳2.7600-020型和Vitros-350型生化分析仪测定血清电解质结果的比对分析 [J], 孙丽3.日立7600-110生化分析仪电解质模块应用评价 [J], 刘玮;刘克芹4.日立7020与7600-020生化分析仪测定血清总蛋白结果可比性分析 [J], 马强虎;王淑萍5.日立7180E生化分析仪电解质模块性能评价指标的研究 [J], 龙波;吕薇;陈梨春因版权原因,仅展示原文概要,查看原文内容请购买。
广东印刷在2009年亚洲标签展上,太阳机械共展示了2台印刷理念完全不一样的设备,显示出太阳机械
的强大的原创设计和制造能力,全面展示其创新理念。
一台是日本原装TLM-250-6C 组合式印刷机,设备
功能同布鲁塞尔类似,但操作性更简便,版筒所有调整均
通过伺服马达控制,例如:版筒压力调整,径向套准,轴向
调准,以及网纹辊自转等都由伺服马达驱动,使得上述调
准都控制在可回复范围内,并且整个结构的记忆使得设备
能够判断被置入的版筒是否与印刷方式是对应的,而版筒
的拆装也十分方便,经过更换的版筒无需重新套准调整,便捷的操作方式在现场得到了业界人员的广泛好评。
另一台展出的设备是T LC-250-4C+1F 卫星式不干胶轮转印刷机。
该设备是传统卫星式轮转印刷机的简化型,在保持原有卫星式轮转印刷机各项性能的前提下,更具有投资小,材料浪费少,操作简便等特点,受到了观摩者甚至已经拥有卫星式设备的用户的极大关注,所展示的设备已经开始了销售。
现场作为电池标专用生产设备,演示内容也是该用户今后的主要产品。
这种相对低损耗
高产能的卫星式轮转设备将会吸引更多的投资者。
(刘霁)
在“2009年亚洲标签展”上
太阳机械全面展示创新理
念
厂商园地
太阳机械52010.17--。
【收稿日期】2008-07-14【作者简介】刘宏博(1976-),女,讲师,硕士,从事细菌耐药机制研究,E m a i l :l i u h b @c m u 2h .c o m ;李胜岐,通讯作者,男,教授,博士生导师,从事细菌耐药机制研究文章编号:1005-376X (2009)01-0048-02【论 著】鲍曼不动杆菌生物被膜形成和菌体形态变化的研究刘宏博,谷秀,李胜岐(中国医科大学附属盛京医院第一呼吸内科,辽宁沈阳 110004) 【摘要】 目的 观察鲍曼不动杆菌能否以生物被膜菌的方式存在。
方法 采用平板改良法,分别于24h 、2d 、3d 、5d 和7d 五个时间点,用扫描电镜观察硅胶膜上是否有鲍曼不动杆菌附着并形成生物被膜。
结果 鲍曼不动杆菌可以生物被膜菌的方式存在,第3天时在硅胶膜上生物被膜成熟。
同时发现细菌形态在被膜形成后发生改变。
结论 鲍曼不动杆菌可以生物被膜菌的形式存在。
【关键词】 鲍曼不动杆菌;生物被膜【中图分类号】R 378.99 【文献标识码】AT h e o b s e r v a t i o n s o f t h e b i o f i l mf o r m a t i o n a n d t h e c h a n g e o f b a c t e r i a f o r mi n A c i n e t o -b a c t e r b a u m m a n n i iL I UH o n g -b o ,G UX i u ,L I S h e n g -q i(D e p a r t m e n t o f R e s p i r a t o r y M e d i c i n e ,S h e n g j i n gH o s p i t a l A f i l i a t e d t o C h i n aM e d i c a l U n i v e r s i t y ,S h e n y a n g 110004,C h i n a )【A b s t r a c t 】 O b j e c t i v e T o o b s e r v e w h e t h e r A c i n e t o b a c t e r b a u m m a n n i i c o u l df o r m t h e b i o f i l m (B F ).Me t h o d s T h e m o d i f i e d f l a t -b o a r d m e t h o d a n d s c a n n i n g e l e c t r o n m i c r o s c o p y w e r e u s e d t o o b s e r v e w h e t h e r t h e B Fc o u l d f o r mo n s i l i c o n d i s k a t 24h o u r s ,2d a y ,3d a y ,5d a y ,7d a y r e s p e c h i v e l y .R e s u l t s A c i n e t o b a c t e r b a u m m a n n i i c o u l d f o r mB Fo n s i l i c o n d i s k a t t h e t h i r d d a y .A n dt h e b a c t e r i a w e r e f o u n dt o h a v e m o r p h o l o g i c a l c h a n g e da f t e r t h e B F f o r m a t i o n .C o n c l u s i o n A c i n e t o -b a c t e r b a u m m a n n i i c a nf o r mB F .【K e y w o r d s 】 A c i n e t o b a c t e r b a u m m a n n i i ;B i o f i l m 近年来鲍曼不动杆菌在临床标本中的分离率和耐药率逐年增高,已成为医院获得性感染的重要病原菌之一[1]。
Hitachi H-25 Gas Turbine in Oil and Gas Market 14Hitachi H-25 Gas Turbine in Oil and Gas MarketOVERVIEW: The Hitachi H-25 is a heavy-duty 30-MW class gas turbine.The first unit started operation in 1989 at a domestic petrochemical plant and total orders for the turbine have reached to 120 units. The H-25 is used not only in Japan but all over the world in various fields such as at power,industrial, and oil and gas plants. The oil and gas market is growing strongly but has special characteristics and extremely severe requirements including special specifications and demand for high reliability. The high performance,reliability, and flexibility of the H-25 gas turbine have helped this field grow by meeting customer demands.Shunichi Kuba, Dr. Eng.Koji Y asuda Y asuhiro Katou Kenji KaminoINTRODUCTIONHIGHER performance and efficiency are an eternal theme in gas turbine applications. In addition to the technologies needed to meet these requirements, the increased awareness of global environmental problems in recent times has also led to demand for improved environmental performance.Against this background, combined plants [consisting of a gas turbine, HRSG (heat recovery steam generator), and steam turbine] have become a standard feature of large power plants operated by electricity companies. These combined plants use bigger gas turbines powered by clean fuels [LNG (liquefied natural gas) or NG (natural gas)] to achieve high efficiency and minimum environmental impact.On the other hand, the medium-sized gas turbine systems used at industrial plants, regional heat and power utilities, refineries and LNG plants, and elsewhere require features such as high reliability and fuel flexibility as well as high performance.This paper discusses the characteristics of the oil and gas market and how the H-25 plays an important role in this market.CHARACTERISTICS OF OIL AND GAS MARKETKey features of the oil and gas market are summarized below.ReliabilityThe most important factor in this field is reliability because the profitability of refineries and chemicalplants, which are huge and complex systems, is dependent on continuous operation without plant shutdown. Therefore, only companies that have products which meet this criteria can enter this field.The company must have appropriate engineering capabilities, maintain detailed operational records, and be able to cover company liabilities, so that it is qualified by major oil companies.In addition, after an order is received, various factory tests need to be performed to verify the reliability prior to shipment. It is common for gas turbine vendors to ship units without running tests at the factory because gas turbines are fully standardized machines and no design changes are made to the gas turbine components such as the nozzle, blades and casing. Only auxiliary systems such as the starting mechanism, lube oil system, and instrumentation are modified for specific projects. However, in the oil and gas market, running at no-load mechanical test of the gas turbine at the factory is a minimum requirement.In some cases, a load test must be performed at a vendor’s test facility. In the severest case, full load string testing is performed using the project generator and auxiliaries such as the inlet system. The purpose of this testing is to avoid trouble at the site and to prove the performance of gas turbine.High PerformanceOnly qualified companies can bid for these projects,and only the vendor who submits the most attractive proposal gets the order. A recent trend is to consider overall cost-performance. Because reliability is theHitachi Review Vol. 58 (2009), No. 1 15During the first decade after the delivery of the first unit, the H-25 has been mainly used in cogeneration plants at domestic petrochemical companies. Based on its proven capabilities and its track record during that period, Hitachi delivered the first unit for overseas use to the Republic of Korea in 2000. Since then, order volumes have grown rapidly with total international orders reaching 120 this year (see Fig. 1). The figures include five H-15 turbines. The H-15 is a scaled down model of the H-25.Table 1 shows the industries where the H-25turbines are used. As shown in the table, the oil andmost important factor, the oil and gas market has long been characterized by the use of heavy-duty machines with low firing temperatures. However, demand for higher performance has increased recently, and aero-derivative gas turbines are also used as a response to the increasing cost of fuel. The cost-performance is evaluated not only on the output and heat rate but also maintenance and other costs in relation of gas turbine operation.Fuel FlexibilityFor environmental and performance factors, NG is the dominant fuel used in gas turbines at power utility companies. However, in the oil and gas or petrochemical fields, many types of fuel such as distillate, LPG (liquefied petroleum gas), and off-gas are used in addition to natural gas. Dual fuel systems (gas/oil, gas/gas) are also popular due to plant operation flexibility.The most difficult fuel to use is off-gas. Off-gases are by-products from a refinery or chemical plant. This gas is cheap and, if it can be used as a fuel of gas turbine, it is beneficial to the oil company because they are a very economical fuel. However, off-gases normally include heavy gases such as propane,sometimes include corrosive elements which are difficult to handle because the component gases can vary widely.Special SpecificationsProjects in this market often involve special specifications. Typical examples are the API (American Petroleum Institute) standards, a set of usual standards in the petrochemical industry. These standards include not just the main API 616 standard (Gas Turbines for the Petroleum, Chemical, and Gas Industry Services). All the related API standards for lube oil systems, gears, heat exchangers, pumps, and similar are referred to in the specifications. One of the purposes of these high-grade requirements is to achieve the highest level of reliability. It is very important to comply with these specifications. In addition to the API specifications, special specifications used by major oil companies, such as the DEP (Design and Engineering Practice) of Royal Dutch Shell (Shell)standards, are also used. These extensive standards impose very severe requirements.HISTORY OF H-25 GAS TURBINEIn 1988, the first H-25 was delivered to the Tokuyama Oil Refinery of Idemitsu Kosan Co., Ltd.Fig. 1—H-25 Gas Turbine Order Records.The graph shows deliveries of H-25 gas turbines in Japan and overseas. H-25 gas turbines have been operating reliably in various different environments ranging from the extreme cold of Russia (–48°C) to the intense heat of Iraq (54°C)T ABLE 1. H-25 UsersThe table shows breakdown of H-25 users.H-25H-15Overseas : 92 unitsDomestic: 28 unitsTotal : 120 unitsHitachi H-25 Gas Turbine in Oil and Gas Market 16gas industry represents the largest market.FEATURES OF H-25 GAS TURBINEDesign Philosophy and Structural FeaturesThe H-25 is a 30 MW class heavy-duty, single-shaft gas turbine with horizontal split casings. Fig. 2shows a cross section of the H-25.Heavy-duty gas turbines are developed primarily for industrial use and have the following advantages over aero-derivative gas turbines.(1) Continuous operation (reliability)(2) Ease of maintenance (site maintenance)(3) Fuel flexibilityA heavy-duty gas turbine consists of three parts: a compressor, a turbine, and a combustor. Table 2 lists the characteristics of each component.Fig. 3 shows the cross section of a typical LNC (low NO x combustor). The total number of LNC units delivered has already reached 40. The latest LNC technology used in the H-25 achieves performance of 18.7 ppmvd @ 15% O 2 available.Fig. 4 shows the 1st stage bucket with TBC (thermalFig. 2—H-25 Cross Section.H-25 gas turbine has simple structure which consists of three main parts, compressor, combustor and turbine.T ABLE 2. H-25 Structural FeaturesThe table shows specifications of main components.Fig. 3—LNC Cross Section.LNC (low NOx combustor) consists of diffusive combustion (F1)and premixed combustion (F2) nozzles.Compressor Combustor TurbineCompressor discharge air(F1) nozzle TransitionpieceTo turbineFig. 4—TBC Technology for H-25 Gas Turbines.By appling TBC on bucket, temperature of the substrate will be decreased, and its reliability will increase.Ceramic layerTBC: thermal barrier coatingbarrier coating) and its theory of operation. Use of a ceramic coating with high heat resistance on the substrate (base metal) means the surface temperature of the substrate is lower than when TBC is not used. It is estimated that use of TBC reduces the temperature by more than 50°C. This increases the reliability of the material for the same level of firing temperature.It can also provide higher efficiency because the firing temperature can be increased while still keeping the substrate at the same temperature.Special FeaturesThe H-25 has the following special characteristics.High reliabilityMore than 70 units are now in commercialHitachi Review Vol. 58 (2009), No. 1 17cogeneration plants and combined plants to run at maximum efficiency.Table 5 shows the performance of the H-25. The H-25 achieves an output of 31 MW and gross thermal efficiency of 34.8 % LHV (lower heating value) when fired with natural gas. This performance is remarkably high for a heavy duty gas turbine in this range. It allows cogeneration plants and combined plants to run at higher efficiency. In a combined cycle plant, a gross thermal efficiency of 50 % or more is possible.EXAMPLE APPLICATIONS IN OIL AND GAS INDUSTRYSakhalin II Project in RussiaThis is a well-known big project (see Fig. 5). Not only is the equipment exposed to severe environmentaloperation, and the total operating time of these turbines exceeds 1.4 million hours. A project to research the reliability of continuously operating H-25s in Japan was carried out in 2006. Three units with an operating time in excess of 100,000 hours were selected. Table 3 summarizes the reported results. The RF (reliability factor) is calculated in accordance with ISO3977-9.The results in the table demonstrate the extremely high reliability of the H-25.Fuel flexibilityTable 4 lists the fuels used in the H-25. The table indicates the importance of fuel flexibility in this market. The fuels used include natural gas, distillate,and LPG. The difference of 44 between the total number of H-25 turbines in service and the number of different fuels used indicates the number of dual fuel units. One important fuel is off-gas generated as a by-product at oil and gas plants. If it can be used in the gas turbine, off-gas is a very cheap fuel source. On the other hand, the content of the off-gas can vary widely and may include corrosive substances or drains.It is very difficult to handle such a fuel and use it as a suitable auxiliary fuel system that incorporates a gas fuel booster system. The engineering capability to do this is the key technology.High level of engineering capabilityAs mentioned in “CHARACTERISTICS OF OIL AND GAS MARKET,” gas turbines often require special specifications such as API and major oil company specifications. Hitachi can supply the best solutions to these requirements. In addition, the gas turbines used at oil and gas plants are normally purchased through engineering companies such as M.W. Kellogg Limited (Kellogg), Toyo Engineering Corporation (TEC), JGC Corporation (JGC), and Chiyoda Corporation (Chiyoda). These engineering companies are familiar with these severe specifications and require considerable engineering documentation to verify quality at both the quotation stage and during the project stages after the order has been made.Satisfying these demands requires the highest level of engineering capabilities. That is a reason why Hitachi has been able to supply H-25s successfully to Kellogg,TEC, JGC, Chiyoda and in addition major oil companies such as Shell and Petrobras Brasil.PerformanceBased on modern materials, coatings, cooling and aerodynamics technologies, the H-25 has the highest thermal efficiency of any heavy-duty 30 MW class gas turbines. The efficiency of the H-25 enablesT ABLE 3. H-25 Reliability RecordThe table shows extremely high H-25 reliability.T ABLE 4. Types of FuelThe table shows fuels used for H-25 gas turbines. 44 units have dual fuel system.T ABLE 5. H-25 PerformanceThe table shows H-25 performance at ISO conditions.RF: reliability factorLPG: liquefied petroleum gasLHV: lower heating valueHitachi H-25 Gas Turbine in Oil and Gas Market 18Fig. 8—Electrical System of Betara H-25 Plant.Three H-25 gas turbines are connected to 13.8 kV line andsupply electricity to the motors for gas compressors.conditions, but it must also comply with the most severe Shell specifications. The minimum ambient temperature reaches –48°C. In order to endure such severe conditions, material selection and systematic design were carefully done. The facility has four sets of H-25 gas turbines and heat recovery units in total.All of these use dry low NO x combustors. Also, two of the sets are dual fuel (gas and oil) units to increase operational flexibility.Damietta Plant in EgyptThis plant is located in a hot desert area. Hitachi received an order for five H-25 sets from the well-known Kellogg engineering company (see Fig. 6). The order specified the API specifications and special measures for dealing with the sandy conditions were required.Fig. 5—H-25 Gas Turbines in Sakhalin II Project in Russia.Hitachi delivered four H-25 gas turbines generation equipment units to the Sakhalin Energy Investment Co., Ltd., for use in a land-side plant that processes gas and oil extracted from theseabed off the eastern shore of Sakhalin Island in Russia.Fig. 6—Overview of Damietta H-25 Plant in Egypt.Hitachi delivered five H-25 gas turbine generator sets toDamietta LNG plant through Kellogg, an engineering company.Fig. 7—Overview of Betara H-25 Plant.Hitachi delivered three H-25 gas turbine generator sets toBetara plant in Indonesia.Betara Project in IndonesiaThis is an example of using the H-25 in a gas booster compressor plant (see Figs. 7 and 8).Hitachi received an order for three H-25 sets from Petrochina International Jabung. This plant receives gas and liquid from tens of nearby gas fields, then separates the liquid from gas to produce refined natural gas. The plant requires hot gas to recondition the molecular sieve of the drier which removes water from the processed gas and uses exhaust heat from the H-25. Each of the three H-25 units is equipped with aHitachi Review Vol. 58 (2009), No. 1 19 ABOUT THE AUTHORSShunichi Kuba, Dr. Eng.Joined Hitachi, Ltd. in 1979, and now works at the Turbine Design Department, Hitachi Works, the Power Systems. He is currently engaged in gas turbine system design. Dr. Kuba is a member of the Japan Society of Mechanical Engineers (JSME).Koji Y asudaJoined Hitachi, Ltd in 1971, and now works at the Thermal Power Systems Division, the Overseas Thermal Power Business Development Department, the Power Systems. He is currently working on sales engineering for gas turbine power generation. Mr. Yasuda is a member of the Gas Turbine Society of Japan (GTSJ).Y asuhiro KatouJoined Hitachi, Ltd. in 1980, and now works at the Turbomachinery R&D Center, the Energy and Environmental System Laboratory, the Power Systems. He is currently engaged in the development of turbomachinery technology. Mr. Katou is a member of JSME and GTSJ.Kenji KaminoJoined Hitachi, Ltd. in 1980, and now works at the Nuclear & Thermal Power Department, the International Operations Division, the Marketing Group, the Power Systems. He is currently engaged in Iberia & Latin America/H-25 Gas Turbine project.WHRU (waste heat recovery unit) that treats the processed gas and uses it for reconditioning the molecular sieve. The refined natural gas produced by the plant is carried by pipeline for use as city gas or fuel for power generation, while the separated hydrocarbon liquid is sent to another plant for separation and refining into LPG for export.Standard practice in gas compression plants has been to use mechanically driven gas turbines with two shafts. A special feature of this plant, however, is that H-25s which drive generators and motor-driven compressors are used instead of mechanically driven gas turbines with two shafts. This system was selected for reasons of total plant availability and maintenance cost. Such a system is only made possible by use of the highly reliable H-25.CONCLUSIONSThis paper has introduced the Hitachi H-25 gas turbine and explained how the H-25 gas turbine hasplayed an important role in the oil and gas market.The increase in the number of turbines delivered since2000 when overseas promotion first started isremarkable. The number has reached 110 units out ofa total of 120 units. We believe this is because thereliability and high performance of the H-25 havebecome recognized in the market, especially in the oiland gas market. We would like to contribute to growingthe oil and gas market through endless efforts toachieve higher reliability and performance.REFERENCES(1)Y. Kojima, “Latest Technical Trends in Heat-resistant Coating,”A Collection of Documents for the 34th Gas Turbine Seminar(Jan. 2006).(2)T. Saito et al., “Development of Low-NOx Combustors Basedon DME and LNG,”Japan Gas Turbine Society, V ol. 34, No. 5(Sep. 2006).(3)H. Tsuruta et al., “H-25 Gas Turbines for PetroChina’s BetaraComplex Development in Indonesia,”Hitachi Hyoron88,pp.221-224 (Feb. 2006) in Japanese.(4)I. Takehara, “Power Generation Systems Based on GasTurbine,”Japan Gas Turbine Society, V ol. 31(May 2003).。