限制性内切酶一览表

常见限制性‎内切酶识别‎序列(酶切位点)（BamHI‎、EcoRI‎、HindI‎I I、NdeI、XhoI等‎）Time：2009-10-22 PM 15:38Autho‎r:bioer‎Hits: 7681 times‎在分子克隆‎实验中，限制性内切‎酶是必不可‎少的工具酶‎。

无论是构建‎克隆载体还‎是表达载体‎，要根据载体‎选择合适的‎内切酶（当然，使用T 载就‎不必考虑了‎）。

先将引物设‎计好，然后添加酶‎切识别序列‎到引物5' 端。

常用的内切‎酶比如Ba‎m HI、EcoRI‎、HindI‎I I、NdeI、XhoI等‎可能你都已‎经记住了它‎们的识别序‎列，不过为了保‎险起见，还是得查证‎一下。

下面是一些‎常用的II‎型内切酶的‎识别序列，仅供参考。

先介绍一下‎什么是II‎型内切酶吧‎。

The Type II restr‎i ctio‎n syste‎m s typic‎a lly conta‎i n indiv‎i dual‎restr‎i ctio‎n enzym‎e s and modif‎i cati‎o n enzym‎e s encod‎e d by separ‎a te genes‎. The Type II restr‎i ctio‎n enzym‎e s typic‎a lly recog‎n ize speci‎f ic DNA seque‎n ces and cleav‎e at const‎a nt posit‎i ons at or close‎to that seque‎n ce to produ‎c e 5-phosp‎h ates‎and 3-hydro‎x yls. Usual‎l y they requi‎r e Mg 2+ ions as a cofac‎t or, altho‎u gh some have more exoti‎c requi‎r emen‎t s. The methy‎l tran‎s fera‎s es usual‎l y recog‎n ize the same seque‎n ce altho‎u gh some are more promi‎s cuou‎s. Three‎types‎of DNA methy‎l tran‎s fera‎s es have been found‎as part of Type II R-M syste‎m s formi‎n g eithe‎r C5-methy‎l cyto‎s ine, N4-methy‎l cyto‎s ine or N6-methy‎l aden‎i ne.酶类型识别序列ApaIType II restr‎i ctio‎nenzym‎e5'GGGCC‎^C 3'BamHI‎Type II restr‎i ctio‎nenzym‎e5' G^GATCC‎3'BglII‎Type II restr‎i ctio‎nenzym‎e5' A^GATCT‎3'EcoRI‎Type II restr‎i ctio‎nenzym‎e5' G^AATTC‎3'HindI‎I IType II restr‎i ctio‎nenzym‎e5' A^AGCTT‎3'KpnIType II restr‎i ctio‎nenzym‎e5' GGTAC‎^C 3'NcoIType II restr‎i ctio‎nenzym‎e5' C^CATGG‎3' NdeIType II restr‎i ctio‎nenzym‎e5' CA^TATG 3'NheIType II restr‎i ctio‎nenzym‎e5' G^CTAGC‎3'NotIType II restr‎i ctio‎nenzym‎e5' GC^GGCCG‎C 3'SacIType II restr‎i ctio‎nenzym‎e5' GAGCT‎^C 3'SalIType II restr‎i ctio‎nenzym‎e5' G^TCGAC‎3' SphIType II restr‎i ctio‎nenzym‎e5' GCATG‎^C 3'XbaIType II restr‎i ctio‎nenzym‎e5' T^CTAGA‎3'XhoIType II restr‎i ctio‎nenzym‎e5' C^TCGAG‎3'要查找更多‎内切酶的识‎别序列，你还可以选‎择下面几种‎方法：1. 查你所使用‎的内切酶的‎公司的目录‎或者网站；2. 用软件如：Prime‎r Premi‎e r5.0或Bio‎e dit等‎，这些软件均‎提供了内切‎酶识别序列‎的信息；3. 推荐到NE‎B的REB‎A SE数据‎库去查（网址：http://rebas‎/rebas‎e/rebas‎e.html）当你设计好‎引物，添加上了内‎切酶识别序列‎，下一步或许‎是添加保护‎碱基了，可以参考：NEB公司‎网站提供的‎关于设计P‎C R引物保‎护碱基参考‎表下载（也可见图片‎）双酶切bu‎f fer的‎选择（MBI、罗氏、NEB、Prom e‎g a、Takar‎a）再给大家推‎荐一种新的‎不需要连接‎反应的分子‎克隆方法，优点包括：①设计引物不‎必考虑选择‎什么酶切位点；②不必考虑保‎护碱基的问‎题；③不必每次都‎选择合适的‎酶来酶切质‎粒制备载体‎；④而且不需要‎D NA连接‎酶；⑤假阳性几率‎低（因为没有连‎接反应这一‎步，载体自连的‎问题没有了‎）。

常见分子实验限制性内切酶酶切位点大全公司内部编号：（GOOD-TMMT-MMUT-UUPTY-UUYY-DTTI-AatII 识别位点Acc65I 识别位点AccI 识别位点AciI 识别位点AclI 识别位点AcuI 识别位点AfeI 识别位点AflII 识别位点AflIII 识别位点AgeI 识别位点AhdI 识别位点AleI 识别位点AluI 识别位点AlwI 识别位点AlwNI 识别位点ApaI 识别位点ApaLI 识别位点ApeKI 识别位点ApoI 识别位点AscI 识别位点AseI 识别位点AsiSI 识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI 识别位点BamHI识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI 识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI 识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI 识别位点BsaXI 识别位点BseRI 识别位点BseYI 识别位点BsgI 识别位点BsiEI 识别位点BsiHKAI 识别位点BsiWI 识别位点BslI识别位点BsmAI识别位点BsmBI 识别位点BsmFI 识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI 识别位点BtgZI 识别位点BtsCI 识别位点BtsI识别位点Cac8I 识别位点ClaI识别位点CspCI 识别位点CviAII 识别位点CviKI-1识别位点CviQI 识别位点DdeI 识别位点DpnI 识别位点DpnII 识别位点DraI识别位点DraIII 识别位点DrdI 识别位点EaeI 识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI 识别位点EcoO109I识别位点EcoP15I 识别位点EcoRI 识别位点EcoRV 识别位点FatI 识别位点FauI 识别位点Fnu4HI 识别位点FokI 识别位点FseI 识别位点FspI 识别位点HaeII 识别位点HaeIIIHgaI 识别位点HhaI 识别位点HincII 识别位点HindIII 识别位点HinfI 识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IVHpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI 识别位点NaeI识别位点NarI识别位点NciI识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII 识别位点NlaIV 识别位点NmeAIII 识别位点NotI 识别位点NruI 识别位点NsiI 识别位点NspI 识别位点PacI识别位点PaeR7I 识别位点PciI 识别位点PflFI 识别位点PflMI 识别位点PhoI识别位点PleI识别位点PmeI 识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI 识别位点PvuI 识别位点PvuII 识别位点RsaI 识别位点RsrII识别位点SacI 识别位点SacII 识别位点SalI 识别位点SapI 识别位点Sau3AI 识别位点Sau96I 识别位点SbfI 识别位点ScaI 识别位点ScrFI识别位点SexAI 识别位点SfaNI 识别位点SfcI识别位点SfiI识别位点SfoI 识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI 识别位点SpeI 识别位点SphI识别位点SspI 识别位点StuI 识别位点StyD4I识别位点StyI 识别位点SwaI 识别位点Taq αI识别位点TfiI 识别位点TliI识别位点TseI 识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点与大家共享（非原创）。

限制性内切酶酶切位点汇AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsgI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsaI识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TliI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

专业资料分享A系列Aatll识别位点…G ACGT匕…一丁3：.. CJGC AG,, .5^Acc65l识别位点5…GFMK AC …3，3一. CA KMJG. . 5JAccl识别位点5…Gl\lK AC …丁3J. . CA KMJG. . 5JAcil识别位点5 …dtGC …3’ 宙…GG空…莎Acll识别位点5 …A A\；GTT .33\ .. T TG* A (X)Acul识别位点5；. CTG A AG(N). \ . . 3J 3d.. ,GACTTC(N)U A... 5J Afel识别位点5\.. AGC^CT..,3,3 …TCGJCGA..,5JAflll 识别位点5 …(fTT A AG…3，3：…G A A T T& …5"Afllll 识别位点5；.. ^CRYGT , ,3 3：…TGYRqA. . ,5Agel识别位点5P. . /fCCGGT , . .3J3；. . TGGCC^A. , .5^ Ahdl识别位点5P. G ACNN F/N NGTC... 3P 3- CTGNI^NNNCAG^.S^ Alel识别位点5；. .CACNt/NNGTG 3' 3. ..GTGNI^NNCAC, .. h Alul识别位点5 . .. A(jfcT….3P 3…TQSA-SAlwl识别位点5 r,GGATC(N)7「3"3. .. CCTAGIN)^..^AlwNl识别位点5：. CAGNNN T CTG..3F 3；. GTC^NNNG AC.. .5*Apal识别位点5 .., GGGCtft. ..3P3\., C^CCGGG .ApaLl识别位点5\.. (^TGCAC.. ,3J3 .. CACGTjGApeKl识别位点5P. . . t^CWGC., .3r3\. CGWCjp., .5^Apol识别位点5；,. FLATTY,, ,3J3 , _ Y TTA AJl. T.5"Ascl识别位点5：., GG^bGCGCC, .33 CCGCGCJ J G, .5JAsel识别位点5 . B 4A T't A A T t. , 3J3 . .. T A A TJ A .. 5JAsiSl识别位点5...GCGAl1SGC r. 3r3...CGCJAGCG . .5Aval识别位点可…C TV CGRG…3」3：..GRGCYJ>..hAvall识别位点5：…dfewCC,.,3r3；., CCWG^.. 5JAvrll识别位点5... C T CT AGG .3’…GGATCf…5」Bael识别位点5；^(NJACIN^GTAYCCNjJ 33.^^NJTGjN^CATRGtN)^ .5^识别位点吕,"・G E AT CC…3’BamHl专业资料分享3’…C C T A Gp -.. 5"Banl识别位点吕…G^YRCC^ 33 …CCRYQp^ .5JBanII识别位点5：.. GRGC Y^t …3",C A Y C G R G . ,,Bbsl识别位点5. .,GAAGAC (N)/ . .3r3J. .. 0 T T C T G 减丄…S' BbvCI识别位点5 …C(T T CAGC.”.3*3 …GGAGT A CG.,S JBbvl识别位点5 …GCAGCHN}；…3* 計…CQTCG(N)iar .5^Bccl识别位点5P. . CCATC(N)/,. 33\ , .GGTAG(N). -5rkBceAl识别位点5... ACGGCtNjJ. ,3^3：.. TGCCG(N)F「5*Bcgl识别位点5 . T4N)CGA(NK TGC(N)J . .3J 3；.^(N)GCT(N)；ACG{N)^.b5^BciVl识别位点5. r,GT ATCC (N)J .3F3. ..C AT AGG (N}t^ ..5PBcll识别位点5... T'feATC A,- 3J3：…ACT AGJ., 5JBfal识别位点5：.. (?T AG . .3r苗…GA.C…hBfuAl识别位点5；. . ACCTGC(N)J 3’3. ..TGG ACG(N)^, .5^Bgll识别位点5；. GCCNNNN^JGGC., 3P3 . . CGGr^hlNNNCCG. , 5*Bglll识别位点莎…A T G T…3’3：”. TCTAt^A.. 5rBlpl识别位点5•…G(7TN AGO., -3J3；., CGAN T；CG , .5^Bme1580l识别位点5 . . GKGCWit …才3 . . Q^MCGKG ,5JBmgBl识别位点h…C AC令TC…孑3 . . .GTGJ： AG. . 5*Bmrl识别位点5... ACTGGG(N) T r.3r3... TGACCC(N)4r..5-Bmtl识别位点h…GCTA(?b …3’3\, C/3 ATCG …5」Bpml识别位点5 .… CTGGAG(N)1P T 3r3... GACCTC(N)14j...5rBpulOI识别位点5’…CCT T N AGC …*33\., GGAN^CG .. 5BpuEl识别位点5；.. CT TG AG(N)r T,.3'3\.. GAACTC(N)…A...S'BsaAl识别位点5；.. YAC'GTR . .3^BsaBl识别位点5： ..GATNN^NATC , .3^3：, ,CTANN/1NTAG,. . 5^BsaHl识别位点5P. . .GFitG YC . . ,3^3...C YGC^RG.. .5JBsal识别位点5... GGTCTC (N)/. .3^3... C C A G A G (N)^. . . 5' BsaJl识别位点5 c'b NNGG.. 3J3； GGNNC/3,. 5BsaWl识别位点5 . ., w'bCGGW, 3J3•…WGGC即…hBsaXl识别位点5 . ^NjACfN^CTCCtN),；. 3-3r ..(N)TG(N)r GAGG(N). .，& 丄p ABseRl识别位点5…GAG GAG的「…3"3... CTCCTC{N)-A….5P BseYl识别位点5\, CV： CAGC.,. 3J CT…GGGTQ3…可Bsgl识别位点5；.. GTGCAG^N^T. 3J3 ….CACGTC{Nj i4i.. . 5JBsiEI识别位点5, . , CGRY'fcG,, 3r3 . .. GC A YRGC . ,5"BsiHKAI识别位点5；…GWGCW七■…J3：..C^WCGWG.. .5BsiWI识别位点5.C'feT ACG, . 3r 3：…GC A TGJS. 5’Bsll识别位点5' . , CCNNNN I^INGG. , .3r 3；., GGN\NNNNNCC.. ,5J BsmAI识别位点5r ^GTCTC(N)7 .3ri3 . .. C AG AG(N).…h▲BsmBI识别位点5. r,CGTCTC (N)J .3F3. ..GC AGAG (N)s^ ..5BsmFI识别位点覚…GGGAC(N)(D T . 3* 占…CCCTG(N),-A. . .5JBsmI识别位点5 . . . G A A T G C N* . . 3J3. . CT T AC^SN ..5JBsoBI识别位点5P. ..CVCG AG. .,3P3 . ^GRGCY^C . .5^Bsp1286l识别位点5 ….GDGC“b_・F 3〔…qHCGDG …5’BspCNI识别位点5\. CTCAG(N)/ . .3 3：…G AGTC (叽…BspDI识别位点5\ . A T T C G A T , , . 3"3：…T A G qj A「SBspEI识别位点5 …T T CCGGA. . ,3J3 . r AGGCCJ- . 5JBspHI识别位点5 ... T T C ATGA3’…A G T A CJ…翼BspMI识别位点5. ACC TGC (N)/. r. 33. .. TGG ACGBspQI识别位点5 . GCTCTTClNjJ .33LXGAGAAGtN)*^ r MiBsrBI识别位点5 . . . CCdtTC, . .3r3 GGC^pAG. . .5^BsrDI识别位点5；. . GC AATGNN*, .3r3 ：…C G T T A QN N …乳BsrFI识别位点5；, .R^ CGG Y . .. 3"3 ., YGGCC^R …5“BsrGI识别位点5 . lIST ACA .3…AC ATGJ…创BsrI识别位点5：.. ACTGGN T, r孑3：. . TG ACJDN .,. 5^BssHII识别位点5P.., G*CGCGC .. ,3P3.,.CGCGqp.,5^BssKI识别位点5 . . .'tCNGG . .3：.. GGNCq…hBssSI识别位点5 .(T A CGAG ,, .3"3F GTGCTJC .. .5^BstAPI识别位点5..GCANNNIS T NTGCCGT\NNNNACG8G5 阴BI识别位点5 . k. T T'b G A A t, 3J3. . AAGCJ T T5JBstEII识别位点5：.. G'fe T N A C C .. . 3^3 ... CCAhl TGJS.. .5^BstNI识别位点5：..cd\VGG. . . 3P3 . ..GGWCC . . 55：..CTCAG(N)tt^-专业资料分享BstUI识别位点5：.. CG'faG,. .3^3 . .. GCJ3C.. .5^BstXI识别位点5 ANNNNI S T N TGG.,3J3 . f.GGTN A NNNNNACC (5)BstYI识别位点5…R先ATdS3\, YCTAG^R.. .5JBstZ17l识别位点5…GT fT AC…卽3\. CA T^ATG.. .5-Bsu36l识别位点5.…CC?TN AGG. . . 3r3-…G G A N T& C. 5"Btgl识别位点5；,. C?bHYGG,. .33；, GG YRC^C , ,5JBtgZI识别位点5…GCGATG㈣「…3」3；..CGCTAC(N),4J...5,BtsCI识别位点5；. .GCAGTG N N T33：.. C G T C A (^N N ... 5'BtsI识别位点5.,,GC AGTG N N T.3P3；.. C G T C A <^N N ...Cac8l识别位点GCN T NGC . 3J3：…CGN/ICG…密ClaI识别位点ATfcGAT.. . 3J3 …T AGCJ A T. 5CspCI识别位点5 :的CAA (N>( GTGG (N)^.,33：・+严JN) GTT (M), CACC(N}^1V，,5J CviAII识别位点5…C T G…:T3 r.. G T A^C . . S PCviKI-1 识别位点5...RG T C Y (3)才…¥C#GR…5’CviQI识别位点S'-.J J TAC ,.3J3：5枣…5’Ddel识别位点5；-. C^TNAG.. .3J3；, G AN\C.Dpnl识别位点Ch,5 G A T C 3 3；. CT AG 5’DpnII识别位点5；r T GATC..33：r, CT AG^ . 5JDral识别位点5；.. T T T^AA …3’3\Rt A A A/TT . , 5JDralll 识别位点5；h CACNNN'bTG. . ,3“3…GT砂Nhl SC…5」Drdl识别位点5 . , GACNNNN T NWGTC, ,3 3：. . CTGNN^NNNNCAG., .5^Eael识别位点5“…gGCCR …;T3 . ..RCCGG/ .. 5JEael识别位点5, ^YfeGCCR, . 3J..RCCGG/ .Eagl识别位点5... C^SGCCG .专业资料分享3 .. GCCGGf. .Earl识别位点5 …CTCTTC (N)t\ .r3... GAGA AG (N)^. ..5PEcil识别位点5. . .GGCGGA(N)J . .3^GCCT (N)S A.., 5JEcoNI识别位点5： . . CCTNt/NNNAGG 33 . . GG ANNN^NNTCC . 5E CO O109I识别位点5P. . R(jfeNCC Y …:TS^.YCCNG/SR . . .5专业资料分享EcoP15l识别位点5 r C AGCAG(N)/ . P3r3 …GTCGTC(Wy 5EcoRI识别位点5 . ., A T T C ... 3 r 3；. . CTT AEcoRV识别位点5.GAT^TC -,3*3：.. CT AJ AG.. .5JFatl识别位点5：・先ATG…3」3\t GTAC^ .. 5’Faul识别位点5J CCCGC (N}J.. .3' 3；.. GGGCG (N)#A. . ,5J Fnu4HI识别位点昭…GC’NGC…朝3... CGN&G -SFokl识别位点5\L.GGATG(N)；1T3J3... CCTACtN),^ .5Fsel识别位点5 …GGCCGG'tc, .33 . CC/3GCCGG . . 5J Fspl识别位点5；,. TGC^bC A .. .3P3 ； ,. A C GJC G T (5)Haell识别位点5 RGCGC T Y. . .33 …LCGCGR …5，HaelllHgal识别位点5 . . .GACGC^N}/. 3... CTGCG<N}1(W. . 5" Hhal识别位点5 …GCdt …3" 宙…中CG…5，Hindi识别位点5：f GT Y T R AC.. .3r3\ .. CAR A YTG fHin dlll 识别位点5；. , A'A G C T T .…3" 3... TTCGA^A., .5JHi nfl识别位点5’ …GklMTC …3 3\., CT NAJG., .5* HinPII识别位点亦…dtGC…:r3 ... CGCp . . 5* Hpal识别位点5. .. GTT> AC.. .T3.., C A AJTG. _5J Hpall识别位点5“...cffeGG (3)3. GGC A C. 5JHphl识别位点51 …GGTGA (N)/.,.3, 3；.. CC AC T (N)7i,., 5" Hpy188l识别位点5 …T G “G A「3A0J CT …£Hpy188lll识别位点5.,TtfNNGA. ..3^3.,AGNN^CT. , ,5JHpy99l识别位点5；.. CGWCG T3：F i GCWGC.. ,5^HpyA V识别位点5.h CCTTC(N)J,3-3：. GGAAG(Nk ...5*%HpyCH4III识别位点h…A C N’G T …3"3 .,. T G^N C A … .5 HpyCH4IVHpyCH4Vm别位点5：. . TC?t A ,. .33■…A QG T…5」Kasl识别位点5.., G^J CGCC.,3P3r.^CCGCG^,..5J Kpnl识别位点5.,, GGT AC't... 3^3■…QCATGG …5，Mbol识专业资料分享别位点5P. .^3ATC .3^3P.. CT AG^. .5^Mboll识别位点专业资料分享5；., GA AGA (N「…3* 3；,, C T T C T (N)J A，. .5* Mfel识别位点5；.. C'X ATTG. ..3^3；T . GTT A Ajp . . 5JMlul识别位点5... /T CGCGT.,，3r3 ：.. T G C G C^A ... 5JMlyl识别位点5 …GAGTC(N, 一33\. .CTCA G (N) . 5-Mmel识别位点5； . .TCC RAC(N)M T.3' 3： . ,AG G YTG(NJ ia>. ..5*MnII识别位点5 ….CC「C (N}/ . 3 3…GGAG㈣立,5-Mscl识别位点5 , F TG(?fc：C A t,3P 3"…ACC^J GT.+J5JMsel识别位点5... f*T A A.. 3P3；T. A A TJ .. . 5^Msll识别位点5 …CAYNNV IN RYG. .,3J3 ...GTRNN^NNYAC (5)MspAII识别位点5P. . . CMCSfcKG, . .3 3•…GKYMC…扩Mspl识别位点5；F. dfcGG …3’3；. . GGqp …5’Mwol识别位点5 . . G C N N N N NVj N G C.. . 3\_ CGNN^NNN NNCG., Nael识别位点5 ... GCC\3GC,. .3r 3：…GGG&GG-.hNarl识别位点5 …GG^t GCC.. .3 3…CCGQGG …5“Ncil识别位点5 .C(7SGG,. ,3r 肚…GGS&C…・h Ncol识别位点5. ,,C T CATGG,. .3*3\ ,. G G T A CJ3 ... 5JNdel识别位点5； ., C /T T A T G ,. 3^ 3... GT A\AC…hNgoMlV识别位点5 . _ (itCGGC,. 3^ 3•…CGGCC^G. . 5Nhel识别位点5 . • T AGO . .,3"3 …GGATqp …hNlalll 识别位点亍…CATCf…3’AG…hNlalV识别位点5\,. GGt/NCC-.3 .. CCN^NGG.. .5JNmeAlll识别位点5：.. GCCGAG{N—G 3 ...CGGCTC(N)ia.^.^5rNotl识别位点5〔GdtGCCGC …;r3 ...CGCCGtyCG (5)Nrul识别位点5 . BT TCG^GA …3J3 …MCFCT …hNsil识别位点5 .ATGC^T T .. .3^ 3... I^ACGTA .. ,5JNspl识别位点5 . RCATG T Y. . 3J 3；.，X h GTACR.,.5rPacl识别位点5...TTAAf r TAA...3-3：+ , A ATJ A ATT T J+5JPaeR7l识别位点专业资料分享5；.. CG AG . ,3P. +G AGCTJC , . 5JPcil识别位点5：…A%島TGT….3*3* . . TGT A CA.. .S,APfIFl识别位点5；, .GAChiVlNGTC .…扩3 . . . C T G N N A N C A G ... 5* PflMI识别位点5； .. C C A N N N wTl T G G.. . 3^ 3；^GGTN A NNNNACC., .5^ Phol识别位点5：...GGfcC (3)3：. CCjjG.. 5JPlel识别位点5\.. GAGTC .3* 3：. CTCAG (N),,. hMiPmel识别位点5：. GTTT^AAC. . .3J3；.T C AAAJ T TG.T 5JPmll识别位点5；,, C AC^TG.. .33P.. GTGJ：AC- .5HPpuMI识别位点5 , h RdTj W c C Y ・”，3“3；_ YCC WGJ3R ., 5J PshAI识别位点5；ACNl7NNGTC . +.3J3 , . .CTGNNJNNC AG . .5'Psil识别位点5；., TT^T A A…X .. A A T A T T ... S HJLPspGl识别位点5 …1tC WGG …3“3；r,GG WCC^ ,.5PspOMI识别位点5；,. (fGGCCC.,. 33：…CCCGGJ3…5’PspXl识别位点5 V(7T CGAGB . b3J3. 8GAGC\CV.. .5*Pstl识别位点5 …CTGC 屆.33；. . G^ACGTC-. S-Pvul识别位点5 …CG ATEG …3" 3；.. GCJ AGC. . . 5JPvull识别位点5；…CAtSfcTG…富. . G T CJ3 A C ... 5^Rsal识别位点5...GT^C (3)3... CAJG.. .5*Rsrll识别位点5；, ,C(?fewCCG.. .3' 3：…GGC W枣C…hSacl识别位点5 GAGCT^ ,3J3；CJCGAG, . 5JSacll识别位点热…CCGC七G 23 .. GGjpGCC . 5JSall识别位点5 G T TCGAC,. 3J 3…CAGCT^S …hSapl识别位点5； .-GCTCTTCtN)^ ,3J 3 . r,CGAGAAG(N)(j.TJ5JSau3AI识别位点5 .. 7G A T c .,. 3^ 3：.. CT AG^..5JSau96l识别位点5... G^SNCC . . 33 …CCNGa ・h专业资料分享Sbfl识别位点TGCA T GG.,y3.., GG/CGTCC , 5’Seal识别位点5：.. A G T鼻C T ...甞3\,. TC AJGA . _5JScrFI识别位点5 ... CCT N GG.. 33\. GGNJSC , . 5JSexAl识别位点5 , .rfbCWGGT., ,3r3 …TGGWCqA. - SSfaNI识别位点5.., GC ATC (N)/ ,.. 3*3J.,. CGT AG (N)9A，.. 5*Sfcl识别位点5：…(?TR¥AG …;T3：. .GAYRT^C. . ,5Sfil识别位点专业资料分享5…GGCCNNNhTNGGCC .,3 3. ,CCGGN/tNNNCCGG .5'Sfol识别位点5；., GGC^CC ... 3J 3：…CCGfGG …5，SgrAI识别位点5 …C R’C CG G ¥ G …3"3. ,.G YGGCC jt AC,,,5HSmal识别位点5；., CCC^GG. . 3r3 …GEGfCC 一5"Smll识别位点S^.C T T Y R AG (3)3"…GARY .C …/SnaBI识别位点5 …T Ad A …3’3P r. A T G^： A T .. S HSpel识别位点5；. . zTCJ AGT . ,3r 3：…TGATC^A …hSphl识别位点5.GCATG'fe.. 3J3... CpT ACG . 5Sspl识别位点5 . A AT^TT 3P3. . . T T AJ AA . .5*Stul识别位点5. R1AGdtCT . ,.3f 3：…TCCJSGA. .5HStyD4l识别位点5 .. T CCNGG.,.『3’…GGNCq…5“Styl识别位点5仁C T CWWG G.. ,3r3；, GGWWC A C..Swal识别位点5 . , , A T T TV A A T ・・33 . h. T A A A J T T A ... 5^TaqaI识别位点5：.. T^G A . . ,33：…AGCJ ., 5JTlil识别位点5... <T T CGAG,,. 3J 3…GAGC"…5"Tsel识别位点5 …gfcwGC …3，3 …CGWQG …Tsp45l识别位点5；, ^STS AC.. 33\. .C ASTG^ . 5JTsp509l识别位点5….V A T T… 3-于…TT A. 5“TspMI识别位点C T CCGGG…3’3’…GGGCCf •…5’TspRI识别位点5 .NNCASTGNN T. ,3J3 .. ^NNGTSACNN., .5*Tth111l识别位点5P. . .GACN T NNGTC..3P3'…GTGN 叫NGAG-hXbal识别位点ftT AGA .3’…A G A T GJ…亍Xcml识别位点ANNNN N T J NNNTGG. 33. . .GGTNN N N^NNNNNACC. . 6Xhol识别位点占…C T TCGAG- 33 …GAGC T& …5"Xmal识别位点专业资料分享5：…CtCGGG…計3；.. GGGCqC . . .5* Xmnl识别位点5..,GA ANNVlN T TC . . 3r 3\ .. C T T N NJM N A A G .,. Zral识别位点5：. ,G AC T GTC■…孑3；, CTG A CAG...5-Tfil识别位点5 , . .G^WTC., .3 3 , . XT W .5。

常用限制性内切酶酶切位点汇总BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsgI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI 识别位点BsoBI 识别位点Bsp1286I 识别位点BspCNI 识别位点BspDI 识别位点BspEI 识别位点BspHI 识别位点BspMI 识别位点BspQI 识别位点BsrBI 识别位点BsrDI 识别位点BsrFI 识别位点BsrGI 识别位点BsrI 识别位点BssHII 识别位点BssKI 识别位点BssSI 识别位点BstAPI 识别位点BstBI 识别位点BstEII 识别位点BstNI 识别位点BstUI 识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIII HgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsaI识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TliI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

限制性核酸内切酶详细资料大全限制性核酸内切酶是可以识别并附着特定的脱氧核苷酸序列，并对在每条链中特定部位的两个脱氧核糖核苷酸之间的磷酸二酯键进行切割的一类酶，简称限制酶。

根据限制酶的结构，辅因子的需求切位与作用方式，可将限制酶分为三种类型，分别是第一型（Type I）、第二型（Type II）及第三型（Type III）。

Ⅰ型限制性内切酶既能催化宿主DNA的甲基化，又催化非甲基化的DNA的水解；而Ⅱ型限制性内切酶只催化非甲基化的DNA的水解。

III型限制性内切酶同时具有修饰及认知切割的作用。

基本介绍•中文名：限制性核酸内切酶•外文名：Restriction endonuclease•作用：切割DNA•别名：限制酶限制性内切酶定义,由来,分布区域,分类性质,用途,命名,类型,第一型限制酶,第二型限制酶,第三型限制酶,生理意义,定义是识别特定的核苷酸序列，并在每条链中特定部位的两个核苷酸之间的磷酸二酯键进行切割的一类酶。

由来一般是以微生物属名的第一个字母和种名的前两个字母组成，第四个字母表示菌株（品系）。

例如，从Bacillus amylolique faciens H 中提取的限制性内切酶称为Bam H，在同一品系细菌中得到的识别不同碱基顺序的几种不同特异性的酶，可以编成不同的号，如HindⅡ、HindⅢ，HpaI、HpaⅡ，MboI、MboⅡ等。

限制性核酸内切酶别名：Endodeoxyribonuclease简称限制酶酶反应限制性内切酶能分裂DNA分子在一限定数目的专一部位上。

它能识别外源DNA并将其降解。

单位定义：在指明pH与37℃，在0.05mL反应混合物中，1小时消化1μg的λDNA的酶量为1单位。

性状制品不含非专一的核酸水解酶（由10单位内切酶与1μg λDNA，保温16小时所得的凝胶电泳图谱的稳定性表示），这类酶主要是从原核生物中分离出来的，迄今已经从近300多种不同的微生物中分离出约4000种限制酶。

Acc65I 识别位点ApaI 识别位点AccI 识别位点ApaLI 识别位点AciI 识别位点ApeKI 识别位点AclI 识别位点ApoI 识别位点AcuI 识别位点AscI 识别位点AfeI 识别位点AseI 识别位点AflII 识别位点AsiSI 识别位点AflIII 识别位点AvaI 识别位点AgeI 识别位点AvaII 识别位点AhdI 识别位点AvrII 识别位点AleI 识别位点AluI 识别位点BaeI 识别位点BamHI 识别位点AlwI 识别位点BanI 识别位点Bme1580I 识别位点BbsI 识别位点BmgBI识别位点BbvCI 识别位点BmrI 识别位点BbvI 识别位点BmtI 识别位点BccI 识别位点BpmI 识别位点BceAI 识别位点Bpu10I 识别位点BcgI 识别位点BpuEI 识别位点BciVI 识别位点BsaAI 识别位点BclI 识别位点BsaBI 识别位点BfaI 识别位点BsaHI 识别位点BfuAI 识别位点BsaI 识别位点BglI 识别位点BsaJI 识别位点BglII 识别位点BseRI 识别位点BspDI 识别位点BseYI 识别位点BspEI 识别位点BsgI 识别位点BspHI 识别位点BsiEI 识别位点BspMI 识别位点BsiHKAI 识别位点BspQI 识别位点BsiWI 识别位点BsrBI 识别位点BslI 识别位点BsrDI 识别位点BsmAI识别位点BsrFI 识别位点BsmBI 识别位点BsrGI 识别位点BsmFI 识别位点BsrI 识别位点BsmI 识别位点BssHII 识别位点BsoBI 识别位点BssKI 识别位点BstAPI 识别位点ClaI 识别位点BstBI 识别位点CspCI 识别位点BstEII 识别位点CviAII 识别位点BstNI 识别位点CviKI-1 识别位点BstUI 识别位点CviQI 识别位点BstXI 识别位点DdeI 识别位点BstYI 识别位点DpnI 识别位点BstZ17I 识别位点DpnII 识别位点Bsu36I 识别位点DraI 识别位点BtgI 识别位点DraIII 识别位点BtgZI 识别位点DrdI 识别位点BtsCI 识别位点HaeIIIHgaI 识别位点HhaI 识别位点HincII 识别位点HindIII 识别位点HinfI 识别位点HinP1I 识别位点HpaI 识别位点HpaII 识别位点HphI 识别位点Hpy188I 识别位点Hpy188III 识别位点EarI 识别位点EciI 识别位点EcoNI 识别位点EcoO109I 识别位点EcoP15I 识别位点EcoRI 识别位点EcoRV识别位点FatI 识别位点FauI 识别位点Fnu4HI 识别位点FokI 识别位点FseI 识别位点HpyCH4III 识别位点HpyCH4IV HpyCH4V识别位点KasI 识别位点KpnI 识别位点MboI 识别位点MboII 识别位点MfeI 识别位点MluI 识别位点MlyI 识别位点MmeI识别位点MslI 识别位点MspA1I 识别位点MspI 识别位点MwoI 识别位点NaeI 识别位点NarI 识别位点NciI 识别位点NcoI 识别位点NdeI 识别位点NgoMIV识别位点NheI 识别位点PmlI 识别位点NmeAIII 识别位点PpuMI 识别位点NotI 识别位点PshAI 识别位点NruI 识别位点PsiI 识别位点NsiI 识别位点PspGI 识别位点NspI 识别位点PspOMI识别位点PacI 识别位点PspXI 识别位点PaeR7I 识别位点PstI 识别位点PciI 识别位点PvuI 识别位点PflFI 识别位点PvuII 识别位点PflMI 识别位点RsaI 识别位点PhoI 识别位点RsrII 识别位点SmaI 识别位点SalI 识别位点SmlI 识别位点SapI 识别位点SnaBI 识别位点Sau3AI 识别位点SpeI 识别位点Sau96I 识别位点SphI 识别位点SbfI 识别位点SspI 识别位点ScaI 识别位点StuI 识别位点ScrFI 识别位点StyD4I 识别位点SexAI 识别位点StyI 识别位点SfaNI 识别位点SwaI 识别位点SfcI 识别位点Taq aI识别位点SfiI 识别位点TfiI 识别位点Tsp45I 识别位点Tsp509I 识别位点TspMI 识别位点TspRI 识别位点Tth111I 识别位点XbaI 识别位点XcmI 识别位点XhoI 识别位点XmaI 识别位点XmnI 识别位点ZraI 识别位点。