Leica DMI4000B智能型倒置荧光显微镜

设备卡片号：070351
设备名称；荧光显微镜工作站
规格型号：Leica DM5000B、Leica DMI4000B
设备单价：658000
设备数量：1
购置时间：2007.12.10
设备状态：正常
设备所在地：
设备负责人：杨俊毅
功能及技术指标：
Leica DMI4000B
主要功能智能型倒置显微镜,
电动6位物镜转换
电动双侧光路出口
色温平衡技术
电动荧光
荧光光强控制，防淬灭
超快速荧光滤块转换
参数显示屏
快捷操作控制键
可配平场消色差物镜或半复消色差,复消色差长工作距离物镜
可配明场,暗场,相衬,IMC（霍夫曼）,荧光,微分干涉功能
10X,目镜,支持25mm视域,或其他倍数目镜,双目或三目观察筒,
12V100W卤素灯透射光照明,
多种物台及样夹
可接多人讨论镜,宏观仪,描图仪,光学变倍等
CO2抚育系统
接CCD或照相装置,与多种软件匹配使用
无限远光路
.Leica DM5000B
产品概述
智能型生物显微镜尖端级正置显微镜,半自动,适用于所有生物或医学应用智能型操作,智能型照明,操作方便易学
7位物镜转换
可配平场半复消色差或平场复消色差物镜
可配明场,暗场,相衬,荧光,偏光,微分干涉功能
旋转式荧光滤块转换5位或8位荧光滤块位,电动转换10X,目镜,支持25mm视域,或其他倍数目镜
双目或三目观察筒
12V100W卤素灯透射光照明
可接多人讨论镜,宏观仪,描图仪,光学变倍等
多种升级可能
接CCD或照相装置,与多种软件匹配使用
触摸显示屏显示所有参数无限远光路。

Leica 荧光显微镜操作指南荧光显微镜型号:Leica DMR生产商:德国Leica公司Leica 荧光显微镜操作指南有关显微镜的的结构:荧光显微镜荧光汞灯:荧光汞灯价格昂贵，样品应集中观察，荧光光源关闭后距离下次开机时间须在30分钟以上，否则会大大降低你观察的效果及灯泡的寿命。

更换新的灯泡后第一次使用必须持续15,30分钟后方可关闭荧光电源。

光圈:孔径光阑挡板，荧光转换光路倾斜器:照明视场光阑:一般调至最大不动变倍器:1.0× ,Bose 一般不用只有在相差时选用。

照相机拍照转换档:不拉出为不能拍照只能观察，拉出一半即能观察又可拍照，全部拉出只能拍照目镜观察不到。

筒: 双目镜HC荧光滤光片 1为普通光2为蓝光3为绿光4为紫外光物镜转盘:100倍为油镜，40倍与100倍之间应有空挡便于加油取片。

(注意: 转换镜头时应转动物镜转盘齿轮，不应转动物镜，否则会导致物镜过紧或过松，易使物镜损坏。

)载物台:上有压片夹(注意:放取样本时应扳动压片夹小夹不应直接扳动压片夹) XY调节机械装置:将样品前后左右移动聚光镜转盘:10倍以上要用聚光镜相差环:H 明场直接观察亮 1、2、3、相差，观察不染色的样品D 暗场主要用于观察金属材料显微镜臂上有焦距调节，粗的调节钮为粗调调节幅度较大，细的调节钮为细调，调节幅度小慢调节。

基座:A孔径光阑高大低小,相差时最大视场光阑一般最大Gray on 光强减少，off 光强增大DLF( Day Light Filter )一般白光滤片N4灰度滤片减少1/4Green:绿色滤片B 调中操作指南:1(使用显微镜前，请打开空调除湿一小时。

若不除湿观察样品所拍的照片中会留下水渍状斑。

2. 打开总电源，打开电脑，打开电脑桌面上的的Adobe Photoshop软件的下拉菜单Import Leica.3 打开显微镜上的电源。

注意只有进行荧光观察时才能打开荧光电源，否则不要打开，以免影响高压汞灯的寿命。

leica dmi8倒置荧光显微镜工作原理
Leica DMI8倒置荧光显微镜工作原理基于倒置的光路径设计，通过逆向放大观察样品。

具体工作原理如下：
1. 光源：Leica DMI8倒置荧光显微镜通常采用激光或者弧光
灯作为光源。

光线通过光源进入显微镜系统。

2. 激发光源与滤光片：激发光源产生的光通过滤光片进行过滤。

滤光片选择能够激发样品荧光的波长，而过滤其他多余的光线。

3. 物镜：经过滤光片后的光线进入物镜。

物镜有不同的倍数，可以放大样品。

光在物镜中被聚焦，形成一个局部的高强度光点。

4. 分光镜：经过物镜后的光线进入分光镜。

分光镜表面涂有特殊的薄膜，将激发光和荧光信号分开。

激发光线被反射到目镜或相机，荧光信号则透过分光镜射向样品。

5. 样品：样品通常是放置在一个液体培养皿或者玻璃底片上。

荧光染料标记的生物标本或者细胞，会在激发光的作用下发出荧光信号。

6. 荧光信号：样品中的荧光染料吸收激发光子，高能激发光子的能量会导致荧光染料的电子跃迁到一个高能态，当电子回到基态时，会释放出激光波长较长的荧光信号。

7. 探测荧光信号：荧光信号通过目镜或者相机进行观察和记录。

荧光信号的特点可以提供有关样品的信息，如位置、结构和代谢活性等。

通过上述步骤，Leica DMI8倒置荧光显微镜能够通过荧光信
号对样品进行观察和分析。

一、运行程序：打开显微镜电源后，双击打开软件二、设定菜单栏禁止设置（非专业人员勿动）三、采集图片：（一）采集基本设置下有有三个设置参数:(Mic即microscope)一）设定：可进行物镜倍数切换，建议重低倍镜到高倍镜进行观察BF：明场，PH：相差，DIC：，POL：，FLUO：荧光Ap:孔径光阑Int.Fine：光强荧光激发光选择：I3（GFP）设置目镜与摄像头分光情况可存储上面拍摄的参数，建议连续拍摄多个样品使用（如芯片）二）:选择调焦（F精细，C粗调）后，把鼠标放在右侧，用鼠标滚动轮进行调焦（先粗调，后精细）载物台移动调节快速/精确图中+为现在拍摄位置，任意点击框中地方，进行快速切换拍摄位置片子任意位置切换对比：在预设位置上，下拉窗口中选择位置1，点击保存更换位置后，下拉窗口中选择位置2，点击保存拉窗口中选择位置1，点击，即可快速切换到位置1（如此类推）三）以下不建议使用：点击后→自动曝光→（不能调节增益）建议：不要使用自动曝光。

曝光时间是指从快门打开到关闭的时间间隔，在这一段时间内，物体可以在底片上留下影像，曝光时间是看需要而定的，没有长短好坏的说法只有需要的讲法。

曝光时间越大，摄像头反应就越长。

对于很弱的信号，曝光也不能无限增加，因为随着曝光时间增加，噪音也会积累。

增益：拍摄图片时，增益不能调，需要放到最小。

增益稍大一些，图像的噪声就出来了。

画质反而不好。

增益越大噪音越大，同等质量下的图片信噪比越低，图像质量越差。

在不过曝的前提下，增加曝光时间可以增加信噪比，使图像清晰。

（拍摄荧光时，用到该原理）饱和度是指的色彩饱合度。

适可而止。

调小了没色，调大了也会有噪声。

建议参数1.5伽玛值的意义是增强亮部反差（伽玛值小于1）或者增强暗部反差（伽玛值大于1）。

是一种非线性的亮度调整。

即伽马调整是部分增强图片暗的部分和亮的部分的对比所以拍摄荧光照片时，将伽玛值调大会感觉能增加暗荧光的亮度。

建议参数0.6/1免疫组化拍摄方法：1.选择BF，5X倍镜下观察，调焦2.调节曝光时间，至适中（通过观察柱状图，为正态分布）。

Leica DM5000B 荧光显微镜使用注意事项
1.观察明场只开启显微镜电源，观察荧光，显微镜和汞灯电源都需要开启；
2.汞灯开启>0.5h才能关闭，关闭>0.5h才能再次开启；
3.观察明场请用桌面LAS V
4.0软件，观察荧光用LAS AF软件；
4.换物镜时，应使用物镜转盘，禁止硬掰物镜镜头；
5.聚焦过程中，严禁用力过猛压碎载破片或损坏镜头；
6.观察过程中需要裸手操作；
7.数据保存
图片禁止保存在桌面，请在新加卷E盘下以“时间和姓名的拼音”新建文件夹并保存；
保存路径不能出现中文汉字；
数据用光盘或格式化的U盘拷贝，未经允许不得删除任何数据或文件；
8. 如使用过油镜，要及时用擦镜纸（光面）沾100％乙醇擦干净；
9. 使用完毕请填写使用记录本，并在“异常情况说明及备注”处注明汞灯的起止时间。

显微镜电源(1.指示灯；2.电源开关) 汞灯电源(1.电源开关；2.汞灯使用时间显示)。

DMI4000B操作规程该机器配有三种观察方法：明场、相差和荧光，显微镜电源开关在独立的控制器CTR4000上。

明场：适合观察本身有颜色的或已经染过色的样品接通电源，开机初始化。

将聚光镜转盘转至BF位置，先手动选择低倍物镜（便于找目标位置通过载物台移动杆），通过调焦旋钮后方由上到下的第二个按键（或BF或CHG-TL）来切换到明场状态（液晶显示屏上看到TL-BF），调小孔径光阑以增强对比。

再调节焦距（先粗调后细调）及灯亮度（机身左下侧的亮度旋钮或聚光镜转盘上孔径光阑）至图片清晰为止.相差：适合观察本身无色或不能染色的样品如活细胞接通电源，将聚光镜转盘下方的孔径光阑拨杆拨至PH位置，手动选择较低倍物镜（便于找目标位置通过载物台移动杆），通过调焦旋钮后方由上到下的第二个按键(或标有CHG-TL或PH)来切换到相差状态（液晶显示屏上看到TL-PH）。

将聚光镜转盘转到相应位置（通常：5倍物镜对应PH0，10X和20X物镜对应PH1, 40X物镜对应PH2位，如位置不对液晶屏上会有闪烁数字提示），再调节焦距（先粗调后细调）及灯亮度（机身左下侧的亮度旋钮）至图片清晰为止。

荧光：按下显微镜左下侧的TL/IL按键直到显示屏上出现fluo，其余操作见后述（第四页）孔径光阑目镜屈光度调节观察方法切换按键拍照：将显微镜右侧调焦轮后面方的分光拉杆拉出，此时光线进入相机，开启显微镜在初始化完成后开启电脑上拍照软件leica qwin（需要加密钥匙，祥见下述：）。

双击桌面Leica Qwin 图标进入软件主界面，点击下拉菜单“图像”→“图像预览”进入预览界面，在该界面下调节好相关参数后点击“采图”将图片拍照进入主界面，再在主界面“文件”→“另存为”将图片保存到目标位置。

预览界面详述“采图”：调好下述参数后点击将活图像采集（就是拍照）到主界面：自动白平衡，点击后软件会对活图像的色彩进行自动调整：自动曝光和手动曝光之间切换，手动曝光时左边的参数显示为曝光时间（改变时间的长短得到一幅明暗适中的图片。

Leica DM ILInverted Microscope –for all Routine and Laboratory Applications2Leica Design by Ernest Igl /Christophe ApothélozLeica DM IL – compact inverted microscope for laboratory routine The new inverted Leica microscope blends ergonomy, a compactdesign and effective contrasting methods into a system for virtuallyunlimited life science applications.The integration of Leica HCS* optics extends the range of objectivesfor inverted microscopy.For the first time, high-quality relief contrast can be producedwithout special objectives with our new Integrated ModulationContrast (IMC) technique.Optimised phase contrast and brilliant incident light fluorescencemake the Leica DM IL the number one choice in contrastingmicroscopes.3With its unbeatable modularity, ergonomy and free view of thespecimen, teamed with newly developed and optimised contrastingtechniques, the Leica DM IL offers you a top level introduction toinverted microscopy.The adaption of the Leica DM IL to infinity optics allows theintegration of Leica HCS* components for superb image resolution,brilliant contrast and precise colour rendering. The DM IL is theinverted equivalent of our successful upright DM microscopes ofthe L and R class.The Leica DMIL is a microscope for all applications in microbiologyor the cell culture laboratory. A universal inverted microscope forroutine use: stable and space-saving, flexible and upgradablewith optics from Leica’s research microscopes.* Harmonic Component System Microinjection of oocytes in mouseRNA microinjection of frog oocytes(Xenopus)4The stand The stand of the Leica DM IL microscope is stable, aluminium-cast and excellently designed. There are two versions for biological applications:The DM IL for brightfield, phase and modulation contrast and the DM IL with additional incident light fluorescence unit.Appreciated by users for many years, the stable T-shaped micro-scope base offers plenty of valuable space round the microscope and ensures comfortable, fatigue-free microscopy. The micro-scope’s footprint is optimised to provide the necessary room for experiments, and all controls are ergonomically located. Many different components can be adapted to suit individual require-ments. The high stability, low centre of gravity and four vibration damping feet eliminate vibrations even in extreme conditions. The excellent stability of the DM IL also makes it the ideal solution for photography with long exposure times. In addition, finite element calculations and thorough practical testing in a wide variety of applications guarantee focusing which is not only ultra precise but also stable over long periods of time.The Leica DMIL contrasting microscopeThe System Discussion unitCardiac muscle cells5Due to its modularity, the Leica DM IL is particularly suitable forliving cell microscopy.Its modern, practical design, the integration of state-of-the-art,top quality optics and the excellent standard of the adaptedcontrasting techniques prove useful in research tasks as well asroutine applications.You will be convinced by the DM IL’s first-class technology andmany innovative and practical ideas.We at Leica believe microscopy should be a pleasurable experienceand designed the DM IL to be associated with enjoyment at thelaboratory workplace.Trinocular tube with DC 100Ergonomic phototubeTrinocular tube with MPS DM IL with illumination column the other way round6Nosepiece focusingSamples are focused with the quadruple objective nosepiece.The reliability, stability and precision of the focusing is not influenced by the microscope stage and the samples on it or by accessory components such as the object guide and manipulators.Illumination system The compact brightfield illumination unit is attached to a column and can be comfortably adjusted. Setting the correct height for the condenser used or the specimen on the stage is facilitated by markings along the column. The pre-centred, extremely powerful 6V 35W halogen lamp provides optimal illumination even of critical specimens. The transmitted light illumination concept is rounded off by the integration of the contrast slide (for phase and modulation contrast), the module for light filters with 32 mm diameter and the aperture diaphragm. The microscope stage with illumination arm can be turned round by 180°and is freely accessable for sample positioning from three sides.Light filters The filter module on the illumination column accommodates 32 mm diameter filters in a spoon-shaped holder. We are constantly adding to our wide range of filters, enabling you to selectively optimise the illumination for observation and image documentation.Built-in 6V 35W power supply The 6V 35W power supply, which provides the full lamp power including on/off switch, status indicator and brightness adjustment,is fully integrated in the microscope stand. Apart from the ergonomic advantages, this saves space on the microscope desk and allows the microscope to be easily picked up as a unit and moved elsewhere.The Technology7CondensersThe Leica DM IL offers you a choice of two condensers.The S 90/0.23 condenser with a free working distance of 90 mm and a numerical aperture of 0.23 is designed for brightfield and phase contrast and is particularly suitable for specimens in bulky laboratory vessels.The S 55/0.35 condenser with a free working distance of 55 mm and a numerical aperture of 0.35 for bulky containers is designed for brightfield, phase and integrated modulation contrast (IMC)and is particularly suitable for higher magnifications or thick specimens.Without a condenser, the maximum free working distance is 200 mm.Stage and accessoriesThe DM IL offers a wide variety of stages with a whole array of accessories and different inserts for your specimen vessels:The standard stage is a fixed stage plate of 252 x 212 mm. The stage can be widened by 70 mm on both sides by adapter plates. The interchangeable stage inserts (20-50 mm) allow smaller petri dishes to be used as well without losing the focus when the objective nosepiece is rotated.Object guides can be attached to both the left and right of the stage and have a minimum adjustment range of 83 x 127 mm. The control of the coaxial drive is in an ergonomically low position so that you can rest your hands on the desk while scanning specimens.The object guides accommodate special and multi-purpose frames for all types of culture vessels.A heating stage up to max. 45°C, a 3-plate mechanical stage and scanning stages round off the range of stages for the DM IL.Micromanipulation ...... and scanning stage8DM LB tubeEyepieces and objectivesThe optics are the heart of every microscope and decisive for the quality of information. We have set new standards here by intro-ducing our HC optics.The Leica DM IL is designed for brightfield, phase contrast, Inte-grated Modulation Contrast (IMC) and incident light fluorescence.All infinity-corrected high performance objectives in the Leica range with 25 mm screw thread are compatible with the DM IL microscope.Even earlier-type Leica objectives can be adapted for use on the DM IL. We offer a wide range of special objectives for inverted microscopy applications with long free working distances (L objectives) and/or with correction mounts (Corr objectives) to compensate for different vessel thicknesses. The latest Leica optics brochure features our whole range of objectives.Depending on the tube configuration, there is a wide choice of eyepieces with magnifications 10x, 12.5x, 15x, 16x or 25x, suitable for fields of view up to 20 mm. Besides special high-point eyepieces for eyeglass wearers, we also supply eyepieces with adjustable eyelens (M eyepieces), into which different types of graticule can be inserted.The DM IL range also comprises many different observation and photo tubes. The tubes are interchangeable and can be individually rotated by 360°in the tube mount and then fixed in position. All tubes are fitted with an infinity tube lens 1x. The following tubes are used on the DM IL:•Binocular tube ILB, with 45°viewing angle, for eyepieces with 23.2 mm outer diameter •Trinocular (photo)tube ILT, with 45°viewing angle, for eyepieces with 23.2 mm outer diameter, with vertical photo/TV exit with switchable light path for either 100% visual or 100% photo/TV.The position of the photo/TV exit 88 mm to the side of the tube has the special advantage that it allows an unobstructed view of the specimen.Other tubes from the Leica DM L range can be used via an IL/L adapter:•Binocular tube HC LB 45°viewing angle •Binocular tube HC LVB 0-35°ergotube •Trinocular (photo)tube HC L1T 45°viewing angle •Trinocular (photo)tube HC L3T 45°viewing angle •Trinocular (photo)tube HC LV1T 0-35°ergotubeThe Optics351+364 mn UVAr 488mn 568mn 647mn Ar/Kr 4905205575766486703504004505005506006507009Developments in diagnostics and med./biol. research (e.g. fluores-cence applications) and the increasing use of video technology and electronic image processing have to be paralleled by intelligent technical adaptations of the microscope system.The new HCS optics concept introduced with the Leica DM R microscope meets this requirement. It is the result of an integral system consideration, harnessing all technological potential.The abbreviation HCS stands for Harmonic Component System.Its special features are:•well-balanced optical and mechanical fitting dimensions•harmonious balance of all optical system components, i.e. the parameters contributing to the microscope’s performance (objectives, tube lenses, tubes, eyepieces, TV cameras/adapters,etc.) have been harmonised throughout the entire optic system.This has created scope for even greater optical opportunities.The HCS system is the answer to your application requirements not only today, but in future, too.Rat testicles, IMC10The Leica DM IL is the microscope for all requirements in the cell culture lab. A universal inverted microscope for routine application: stable and space-saving, flexible and upgradable with optics from Leica research microscopes.BrightfieldThe whole range of objectives from 4x-100x magnification can be used for brightfield applications. Samples in almost any kind of vessel can be examined with or without a condenser, while a 6V 35W halogen lamp ensures optimal illumination.Phase contrastIn vivo/ in vitro microscopy specimens are mostly living cultures or microorganisms and are examined under sterile conditions. Contrast of the transparent tissue can only be enhanced by optical methods.Phase contrast is a useful technique for high-contrast imaging of unstained specimens. The phase contrast technique used by Leica on the DM IL has been optimised for inverted microscopy applications and produces equally excellent contrast in watery solutions and of dry preparations in petri dishes.Spinal cord, catContrastingLymphocyte toxicity testdouble staining, strongly positiveLymphocyte toxicity testdouble staining, weakly positive11Human brain Femtotip microinjection needle (Photo: Eppendorf)FibroblastsIMC (Integrated Modulation Contrast)The innovative technique of Integrated Modulation Contrast (IMC)now introduced by Leica in the DM IL is based on Hoffman’sprinciple and produces this contrast without the need for specialobjectives–ordinary brightfield or phase contrast objectives canbe used. The Leica IMC provides a high-contrast, 3D image oftransparent objects similar to that of interference contrast. Plasticculture vessels do not impair the quality of the image as thetechnique is polarisation-neutral.The diaphragm slide on the side of the illumination and theswitchable modulator in the intermediate image of the pupilproduce the type of contrast named after Hoffman without modi-fying the objectives. High contrast, high resolution, a halo-freerelief image of either stained or unstained specimens make Leica’sIMC a new standard in the class of inverted routine microscopy.FluorescenceThe fluorescence model of the DM IL reflects the growing signifi-cance of fluorescence for in vivo/in vitro microscopy. The maincomponents of this configuration are an incident light axis integrat-ed in the microscope stand, incorporating a fluorescence slide forthree filter cubes. A wide range of light sources with multi-lens,chromatically corrected collectors brighten up even the weakestfluorescence. The fluorescence filter cubes comprise an optimallymatched combination of excitation, reflection, band-pass andbarrier filters. We are constantly updating our range of filtercubes to keep pace with the latest challenges in biology andmedicine.Transmitted light techniques can be used simultaneously or inalternation in order to clearly allocate fluorescent and non-fluorescent structures.The Leica DM IL offers you a powerful system for immunology,cytopathology, virology – in fact wherever fluorescence techniquesare used in combination with inverted microscopy.Leica Microsystems – the brand for outstanding products Microscopes Compound Stereo Surgical Laser Scanning Photomicrography Video Microscopy Measuring Microscopes Advanced Systems Image Analysis Spectral Photometry Automated Inspection Stations Measurement Systems Electron Beam Lithography Laboratory equipment Tissue Processors Embedding Systems Routine- & Immunostaining Coverslippers Refractometers Microtomes Sliding, Rotary & Disc Cryostats Ultramicrotomes EM Sample Preparation Leica Microsystems’ Mission is to be the world’s first-choice provider of innovative solutions to our customers’ needs for vision, measurement, lithography and analysis of microstructures.Leica, the leading brand for microscopes and scientific instruments, has developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Leica symbolizes not only tradition, but also innovation.Leica Microsystems – an international company with a strong network of customer servicesAustralia:North Ryde/NSW Tel. +61 2 9879 9700Fax +61 2 9817 8358Austria:Vienna Tel. +43 1 495 44 160Fax +43 1 495 44 1630Canada:Willowdale/Ontario Tel. +1 416 497 2860Fax +1 416 497 8516Denmark:Herlev Tel. +45 4454 0101Fax +45 4454 0111Finland:Espoo Tel. +358 9 6153 555Fax +358 9 5022 398France:Rueil-Malmaison Tel. +33 1 473 285 85Fax +33 1 473 285 86CedexGermany:Bensheim Tel. +49 6251 136 0Fax +49 6251 136 155Italy:Milan Tel. +39 0257 486.1Fax +39 0257 40 3273Japan:Tokyo Tel. +81 3 5435 9600Fax +81 3 5435 9618Korea:Seoul Tel. +82 2 514 65 43Fax +82 2 514 65 48Netherlands:Rijswijk Tel. +31 70 4132 100Fax +31 70 4132 109Portugal:Lisbon Tel. +351 1 388 9112Fax +351 1 385 4668Republic of China:Hong Kong Tel. +852 2564 6699Fax +852 2503 4826Singapore:Tel. +65 779 7823Fax +65 773 0628Spain:Barcelona Tel. +34 93 494 95 30Fax +34 93 494 95 32Sweden:Sollentuna Tel. +46 8 625 45 45Fax +46 8 625 45 10Switzerland:Glattbrugg Tel. +41 1 809 34 34Fax +41 1 809 34 44United Kingdom:Milton Keynes Tel. +44 1908 246 246Fax +44 1908 609 992USA:Deerfield/Illinois Tel. +1 847 405 0123Fax +1 847 405 0030and representatives of Leicain more than 100 countries.Leica Microsystems Wetzlar GmbH Ernst-Leitz-Straße D-35578 Wetzlar (Germany)Tel. +49 (0)6441-290Fax +49 (0)6441-292599 ©L e i c a M i c r o s y s t e m s W e t z l a r G m b H •E r n s t -L e i t z -S t r a ße •35578 W e t z l a r •T e l. (06441) 29-0 •F a x (06441) 29-2599 G e d r u c k t a u f c h l o r f r e i g e b l e i c h t e m P a p i e r .B e s t e l l -N u m m e r n d e r A u s g a b e n i n : D e u t s c h 913 761•E n g l i s c h 913 762 •F r a n z ös i s c h 913 763 •I t a l i e n i s c h 913 764 •S a c h -N r . 501-068 G e d r u c k t i n D e u t s c h l a n d X I /98/A X /B .H .。

倒置生物显微镜DMI3000B简要操作说明
1.接通电源，通过“开/关”接通显微镜电源。

2.根据使用者的眼间距调节双目镜筒的间距。

3.观察方法：
（1）亮视野观察
A.把聚光器设置到亮视野位置（在0-8范围内调到适当的位置）。

B.插入一个样品在载物台上。

C.先选择低倍物镜，通过右侧的转动杆调整载物台的位置，找到合适的视野。

D.用左侧下方的光线分压器调节亮度。

E.利用调焦操纵轮聚焦图像（左手侧聚焦操纵轮能用于粗略调焦，也可用于
精确调焦；右手侧聚焦操纵轮只能用于精确调焦）。

F.再转换成合适倍数的物镜聚焦进行观察。

（2）相衬观察（推荐用于活体细胞观察）
A.完全敞开聚光器的光圈（调节到ph位置）。

B.插入需要观察的样本。

C.选择一个相衬物镜（标有PH0/PH1/PH2），先用低倍物镜找视野。

D.在聚光器上选择与物镜对应的光圈（选物镜上标有PH0，则要将光圈转盘
转到0位置；标有PH1，则要转到1位置；标有PH2，则要转到2位置）。

E.用左侧下方的光线分压器调节亮度。

F.利用调焦操纵轮聚焦图像。

G.再换成合适倍数的物镜聚焦进行观察。

【注意事项】
1.显微镜系精密仪器，所有切换，动作要轻，要到位，务必仔细耐心使用。

2.如镜头上有指纹或污迹用擦镜纸轻轻将其擦除。

打扫卫生。