高二冠词学生版

冠词1．Organized volunteering and work experience has long been ___ vital companion to ___ university degree courses. A. 不填；the B. a; the C. a; 不填 D. a ; a2．Under______ reform program announced on Nov 15, the government has decided to give____ green light to couples wanting to have two children if one of the two parents is an only child.A. a ; aB. the ; theC. / ; aD. a ; /3．Exploring ________space is exciting, yet dangerous. There is ________need for both robots and humans for space exploration.A. the; aB. a; aC. /; aD. /; the4．There is no easy walk to __________ freedom anywhere，and many of us will have to pass through __________ valley of the shadow of death again and again before we reach the mountaintop of our desires．A．/；the B．the；the C．/：／D．the；／5．As environment protection is becoming _______ public concern, _______ government is determined to pay more attention to the issue.A. 不填；theB. a; 不填C. the; aD. a; the6．______ 6 world powers have accepted ______ Iranian offer for talks on its disputed nuclear program.A. The; aB. 不填; anC. The; anD. 不填; a7．Although it doesn't look like＿，the entire cathedral is made of wood.A. itB. thatC. thisD. such8．Sun Yang,＿Olympic and world swimming champion of China, has received＿indefinite ban from competition following his seven-day detention for driving without a license.A. the; anB. an;／C. an; theD. the;／9．The development of industry has been ____gradual process throughout _____ human existence, from stone tools to modern technology.A. 不填; theB. the; aC. a; 不填D. a; a10．China issued new plan which calls for immediate action to deal with country worsening air pollution.A. the ; aB. a ; theC. a ; aD. a ; /11．—Have you heard of ______ death of Nelson Mandela?--Oh, really? It will be ______shock to the world.A. /; theB. a; theC. /; aD. the; a12．It is reported that recently discovered painting may be Van Goph．A．the；不填B．a；the C．a；不填D．the；a13．Mike spent time with ____native people to gain ____ better understanding of the country.A. the; aB. the; /C. a; theD. /; a14．Some villagers are resting themselves in ____shade.A. aB.anC.theD. /15．The finance minister has not been so _____ since he raised taxes to such a high level.A. popularB. well-knownC. favorableD. preferable16．---I acquired ______ Van Gogh from a businessman from Paris the other day.---Really? Are you sure it’s ______ real one?A. /, aB. /, theC. a, aD. a, the17．Young as he is, David has gained ___________ rich experience in ___________ society.A. the; theB. a; 不填C. 不填; 不填D. 不填; the18．My parents hold fond memories of _______ fashion and music of _______1980s.A. the; /B. /; theC. /; /D. the; the19．Nelson Mandela, who was elected ______ president of South Africa in 1994, is generally regarded as______ father of the country.A. the; theB. /; theC. /; /D. the; /20．Sam has been appointed _______ manager of the engineering department to take ____ place of George.A. /,/B. the; theC. the,/D./, the21．While income worry is _______ rather common problem of the aged, loneliness is another problem that _______ aged parents may face.A. a; theB. the; /C. a; /D. the; the22．If my train is on ____ schedule, I will be home at ____ midnight.A. the ; theB.\ ; theC. a; \D. \ ; \23．Have you got these jeans in larger size? This pair is a bit too small around waist.A．/; the B．a; the C．the; / D．a; a24．My father gave me _______ determined look --- ________ kind that said he would never change his mind.A. a, aB. a, theC. /, theD. the, a25．In Chinese,_______ date 2013--1--4 has a similar pronunciation to "love you for a lifetime", making it _______ most lucky and romantic day．A. a; theB. 不填; aC. the; aD. 不填; the26．All of sudden some passengers on_____board let out screams, running upstairs like crazy.A.a;/B.the;theC.a;theD.the;/27．China's economy and financial market will be on steady path in 2014, Premier Li Keqiang said on Friday during trip to Tianjin．A．a; a B．the; a C．a; 不填D．the;不填28．It can’t be denied that the Diaoyu Islands are _______ part of China and what some Japanese have done recently is actually a defence against _______ Chinese people.A. a; /B. a; theC. the; /D. /; the29．Tom works in a company and gets paid by ______ hour, totally 5,000 yuan ______ month.A. the; theB. a; theC. the; aD. a; a30．–Where can we park ___ car?–Don’t worry. There’s sure to be ____ parking lot nearby.A. the; theB. the; aC. /; aD./; the31．Hiking is _____ great fun. You will get close to ____ nature and take exercise at the same time.A. a; theB. a; 不填C. 不填；theD. 不填；不填32．On seeing ______gift they wanted ，the kids screamed with ______delight.A. /; aB. a；theC. the；/D. the；a33．Have you heard news that he is ranked ________first in the competition?A. a; /B. /; theC. the; theD. the; /34．—Have you heard of _______ death of Nelson Mandela?—Yes. It is indeed _______ loss to the world.A. the; aB. a; theC. the; /D. a; /35．By the end of September 2013，＿＿＿＿．number of netizens in China has hit ＿＿＿record high of 604 million．A. the；the B. a；a C. a；the D. the；a36．People develop ______ preference for a particular style of learning at ______ early age and these preferences affect learning.A. a; anB. a; 不填C. 不填; theD. the; an37．Wang yapping is ______ second woman astronaut in China who reached _____ space.A. /; theB. the; /C. the; theD. a; a38．--Did you enjoy yourself yesterday?--Yes. As you saw, ________ party went on in____________ most pleasant atmosphere.A. the; aB. the; theC. a; theD. a; a39．I have learned that it’s taking _____ long time to become _____ person you want to be.A. the; aB. a; theC. the; theD. 不填; a40．______ Mr. Li is now waiting for you on ______ first floor.A. A; aB. The; aC. The; /D. A; the1. (2014·重庆，6)I can't tell you ________ way to the Wilsons' because we don't have ________ Wilsonhere in the village.2. (2014·浙江，2)The paper is due next month, and I am working seven days ________ week, often longinto ________ night.3．(2014·江西，22)They chose Tom to be________captain of the team because they knew he was________ smart leader.4．(2014·陕西，9) ________ village where I was born has grown into ________ town.5．(2014·天津，8)Life is like________ocean: Only________strong-willed can reach the other shore. 6．(2013·新课标全国卷Ⅰ)India attained ________ independence in 1947, after ________ longstruggle.7．(2013·重庆卷)The parents were shocked by ________ news that their son needed ________operation on his knee.8．(2013·山东卷)It was ________ cold winter night and the moon was shining brightly across________ night sky.9．(2013·新课标全国卷Ⅱ) Four and ________ half hours of discussion took us up to midnight，and________ break for cheese, chocolate and tea with sugar.10．(2013·浙江卷)People develop ________ preference for a particular style of learning at ________early age and these preferences affect learning.1. Jumping out of ________ airplane at ten thousand feet is quite ________ exciting experience.A./; theB./; anC.an; anD.the; the2. There’s ________ dictionary on ________ desk by your side.A.a; theB.a; aC.the; aD.the; the3. An accident happened at ________ crossroads a few meters away from ________ bank.A.a; aB./; aC./; theD.the; /4.The warmth of _____ sweater will of course be determined by the sort of _____ wool used.A.the; theB.the; /C./; theD./; /5.Mr.Li will give us ________ talk.________ talk will begin at 8:00.A.a; TheB.the; AC./; /D.a; A6.The sun gives us ________ heat and ________ light.A.the; theB.a; aC.a; theD./; /7.—What do you need in dinner?—I need ________.A.a knife and a forkB.a knife and forkC.the knife and forkD.the knife and the fork8.________ Mrs.Smith is waiting for you in your office.A.TheB.AC.OneD./9.She’s on ________ People’s Daily.A.theB.anC.aD./10._____ Einsteins could not pay for _____ advanced education that young Albert needed.A.The; theB.A; aC.An; theD.The; an11.She is ________ newcomer to ________ chemistry but she has already made some important discoveries.A.the; theB.the; aC.a; /D.a; the12.—Where is Jack?—I think he is still in ________ bed，but he might be in ________ bathroom.A./; /B.the; theC.the; /D./; the13.I don’t like talking on ________ telephone; I prefer writing ________ letters.A.a; theB.a; /C.the; theD.the; /14.Many people are still in ________ habit of writing silly things in ________ public places.A.the; theB./; /C.the; /D./; the15.—If you don’t like the red coat，take the blue one.—OK，but do you have ________ size in blue? This one is a bit tight for me.A.a bigB.a biggerC.the bigD.the bigger16.I heard somebody playing ________ piano in ________ next room.A.a; aB.the; theC.a; theD.the; an17.________ Pacific Ocean is ________ largest of all.A.the; aB./; theC.the; theD.a; /18.Great changes have taken place in ________ 1990’s.A./B.aC.theD.an19.Wouldn’t it b e ____ wonderful world if all nations lived in _____ peace with one another?A.a; /B.the; /C.a; theD.the; the20.Alexander Graham Bell invented ________ telephone in 1876.A./B.aC.theD.one21.In 1864，Lincoln was elected _____ President of ____ United States for the second time.A.the; theB.a; aC./; theD./; /22.John is ________ university student.A./B.anyC.aD.an23.The table is made of ________.A.a woodB.some woodC.the woodD.wood24.We are going to learn ________ next week.A.Twelfth LessonB.The Twelfth LessonC.Twelve LessonD.The Twelve Lesson25.—I’d like ________ information about the management of your hotel，please.—Well，you could have ________ word with the manager. He might be helpful.A.some; aB.an; someC.some; someD.an; a26.Paper money was in _______ use in China when Marco Polo visited the country in ______ thirteenth century.A.the; /B.the; theC./; theD./; /27.Most animals have little connection with ________ animals of ________ different kind unless they kill them for food.A.the; aB./; aC.the; theD./; the28.Alice is fond of playing _____ piano while Henry is interested in listening to _____ music.A./; theB./; /C.the; /D.the; the29.Oh，John.________ you gave us!A.How a pleasant surpriseB.How pleasant surpriseC.What a pleasant surpriseD.What pleasant surprise30.________ terrible weather we’ve been having these days!A.How aB.What aC.HowD.What31.Many people agree that _____ knowledge of English is a must in ____ international trade today.A.a; /B.the; anC.the; theD./; the32.Beyond ________ stars，the astronaut saw nothing but ________ space.A.the; /B./; theC./; /D.the; the33.—Have you seen ________ pen? I left it here this morning.—Is it ________ black one? I think I saw it some where.A.a; theB.the; theC.a; aD.the; a34.After watching ________ TV，she played ________ violin for an hour.A./; /B.the; theC.the; /D./; the35.—Are you sure to help me find ________ bed for my new house?—Sure，but not now. I’m heading for ________ bed and a good sleep.A.a; /B.a; aC.the; aD.the; /36.Charlie Chaplin was considered one of the greatest actors in ___ history of ___ cinema.A./; /B.a; theC.the; theD.the; a37.—What about ________ book?—It’s too difficult ________ book.A.a; aB.a; theC.the; theD.the; a38.Towards ________ evening ________ cold rain began to fall.A.an; theB.the; aC.the; /D./; a39.She always plays ________ football after ________ school.A.a; theB.the; theC./; aD./; /40._____ wheel is thought to be _____ invention of _____ first importance in human history.A.A; the; theB.The; an; theC.A; an; theD.A; an; /41.I felt someone patted me on ________ shoulder.A.aB.theC.myD./42.—So you are running ________ restaurant?—Yes，but I don’t want to make it ________ restaurant only for rich people.A.the; /B.the; theC.a; theD.a; a43.Xi’an was ________ starting point of________ world famous“Silk Road”.A./;/B.a; aC.the; /D.the; the44.It is ten o’clock in the morning but he is still ________.A.in the bedB.at the bedC.in bedD.on bed45.More than half of ________ water used for drinking，washing and irrigating crops comes from under ________ ground.A.the; /B./; theC./; /D.the; the46.At noon we reached ________ small village ________ east of ________ Summer Palace.A.the; the; theB.a; /; theC.a; the; theD.a; /; /47.The child had only ________ slight temperature，but the doctor regarded ________ illness asserious enough for ________ hospital treatment.A./; /; theB.a; /; /C.a; the; /D./; the; the48.I had ________ lunch at a friend’s house yesterday.________ food was good.A.the; /B./; /C.the; theD./; The49.—What is Jack going to do with all his money?—He says he has always dreamed of taking ________ trip around ________ world.A.a; theB.the; theC.a; /D.a; a50.Qingdao is ________ most beautiful city in summer.A.theB./C.aD.one51.Just now you said you always got to work on _______ 8 o’clock train. But would _______later train get you to work on time?A.a; aB.a; theC.the; theD.the; a52.The building was completed in ______ September of 1956 not in ______ October,1955.A./; theB.the; theC./;/D.the; /53.I would like to have _______ room，_______ window of which opens to _______ south.A.a; the; aB.a; the; theC.a; /; /D.a; the; /54.There is _______ house in the picture.There is _______ old woman near _______ house.A.an; a; theB.a; an; theC.the; a; anD.a; the; an55.________ Europe and ________ America are separated by ________ Atlantic Ocean.A./; /; theB.the; the; theC./; /; /D.the; the; /56.There is ________“h” in the word“honest”.A.aB.theC./D.an57.________ terrible life people in the small island lived at that time!A.WhatB.HowC.What aD.How a58.Father often says to me,“Be _____ honest boy today and _____ useful man tomorrow.”A.a; aB.an; anC.a; anD.an; a59.Things of ______ kind come together; people of _____ kind fall into _____ same group.A.the; the; theB.a; a; theC.the; the; aD.a; the; the60.________ Suez Canal brought ________ east and ________ west closer.A.The; the; theB./; the; theC.The; /; /D./; /; /名词、代词、数词、冠词专练100题1. —Isn’t it getting dark earlier tonight?— I think so. I see ____ is on already.A. the street lightB. the light of the streetC. the street’s lightD. the light street2. They are ____.A. mathematics studentB. mathematic studentsC. students in mathematicsD. students of mathematics3. The shop at the street corner sells ____ clothing.A. child’s and woman’sB. children and women’sC. children’s and womenD. children’s and women’s4. She knows that as a secretary she must be pleasant and helpful no matter how busy she is or what kind of ____ she may be in.A. moodB. mindC. formD. thought5. I was paid last week, but I can’t remember the exact ____ of the money.A. numberB. valueC. amountD. percent6. I have a dream that we will one day live in a ____ where we’ll have a lot of trees and fresh air.A. stateB. countryC. nationD. space7. Our football team’s ____ has been excellent during the whole year.A. demandB. masterC. historyD. performance8. As the plane came down through the clouds, green fields and white houses came into ____.A. lightB. sightC. eyesD. effect9. There are a lot of problems today which are beyond any single person’s ability to solve, and they call for united ____.A. forceB. effortsC. energyD. operations10. He went back to the hall to try his ____ at finding his lost gold watch, but never found it.A. bestB. successC. resultD. luck11. He found a lot of____ were ____.A. passers-by; grown-upsB. passer-bys; growns-upC. passer-bys; grown-upsD. passers-by; growns-up12. He left ____ with my secretary that he would call again in the afternoon. He said he would keep____.A. words; his wordsB. word; his wordC. word; wordD. the word; his word13. To some life is pleasure, but to ____ it is suffering.A. the othersB. othersC. other onesD. those14. I want to know ____: Has Mr Jones been here the whole morning?A. itB. oneC. thatD. this15. He cares so little about his meals that ____ will do so long as it fills his stomach.A. anythingB. everythingC. nothingD. something16. ____ will make a trip around the world during the coming year.A. The BlacksB. The Blacks’C. The BlackD. Blacks17. The public expressed ____ concern about the accident.A. itsB. theirC. herD. his18. Are the products of Taiwan____?A. somewhat like JapanB. something like JapanC. somewhat like that of JapanD. somewhat like those of Japan19. — What about the price of these washing machines?— They are equal in price to, if not cheaper than, ____ at the other shops in the street.A. othersB. itC. thatD. the ones20. My grandparents used to live in ____ is now part of Shanghai.A. whichB. whatC. whereD. that21. Computers must be of great use to ____ doing the scientific research.A. theseB. whoeverC. thoseD. whomever22. Never laugh at the failure of ____, because ____ enjoys being laughed at.A. others; nobodyB. other; everyoneC. the other; anyoneD. others; someone23. You may take any of these oranges. If you don’t like this one, you may take ____.A. the othersB. othersC. anotherD. all the others24. — He was nearly killed by a car once.— When was____?— ____ was in 2000 ____ he was in a middle school.A. that; It; whenB. that; It; thatC. this; That;thatD. this; It; when25. The thieves ran away separately, ____ carrying a bag.A. allB. eachC. everyD. either26. I want very much to buy t his color TV set, but I can’t afford ____.A. oneB. itC. thatD. this27. — Which of the two books will you take?—I’ll take____ and I think ____of them very important to me.A. either; neitherB. neither; bothC. both; eitherD. either; both28. She said she would live in London for ____ four or five years.A. otherB. anotherC. the otherD. the others29. Johnson is a New Yorker; ____ is, he lives in New York.A. thisB. thatC. heD. it30. — Do you want to see my driving license or my passport?— Oh, ____.A. either one will doB. either does wellC. neither doD. each will be fine31. ____ ought to be no trouble because he knew the answers.A. ItB. ThereC. HeD. That32. — Did a letter come for me?— Yes. ____ came for you this morning.A. ThatB. ItC. OneD. The letter33. I sent invitations to 90 people, ____ have replied.A. of whom only 30 of themB. of whom only 30C. only 30 of those whoD. only 30 who34. — Who could do a thing like that?—____ but a brave man, I’m afraid.A. EverybodyB. SomebodyC. AnyoneD. Nobody35. Miss Smith thought ____ a great honour to be invited to speak to all of us.A. thatB. thisC. itD. her36. The population of our village today is 70 percent as much as ____ before liberation.A. theseB. thoseC. themD. that37. When the bell rang announcing the end of class, students came out ____.A. by twos and threesB. by two and threeC. by two or threeD. by twos or threes38. Li Ping was born ____.A. in the year 1984, at 10 a.m. on June 18thB. on June 18th at 10 a.m. in the year 1984C. at 10 a.m. in the year 1984 on June 18thD. at 10 a.m. on June 18th in the year 198439. David helps his mother with the housework every Saturday for about____.A. one and half hoursB. a half and an hourC. an hour and halfD. one and a half hours40. This is a ____building, which is about ____ high.A. six-storey; 38 metreB. six-storeys; 38-metreC. six-storeyed; 38 metresD. six-storey; 38-metres41. The cotton production has increased by ____ percent this year compared with last year.A. five point six eightB. five point sixty-eightC. fifth point and six eightD. five point and six eight42. John spent too much time talking on ____ phone while we were all busy at ____ work.A. the; /B. a; /C. / ; /D. the; the43. You can have ____ second try if you fail ____ first time.A. the; /B. / ; aC. the; aD. a; the44. I didn’t tell him____ news at once because I wanted to give him ____ surprise.A. a; aB. the; theC. the; aD. / ; the45. — Who is your English teacher?— Mr. White, ____ European working in ____ Nanjing University.A. an; theB. a; theC. an; /D. a; /46. When we study a globe, we can see ____ is the largest ocean and ____ the largest continent.A. the Pacific; AsiaB. Pacific; the AsiaC. the Pacific; the AsiaD. Pacific; Asia47. The soldier was wounded in ____, but he caught the enemy officer by____.A. the knee; the armB. his knee; his armC. his knee; the armD. the knee; his arm48. According to the recent reports, one of the rare animals, ____crocodile, is in____danger of dying out.A. the; theB. the; /C. / ; /D. / ; the49. I don’t know who invented ____ telescope, but I think it is ____ most useful invention.A. the; theB. the; aC. a; theD. a; a50. Things of ____ kind come together; people of a mind fall into ____ same group.A. a; aB. a; theC. the; theD. the; a51. We must pay special attention to the idioms of ____ English language.A. theB. someC. anD. /52. My brother and I wanted to see the new play, but we weren’t able to get the tickets for___two of us.A. /B. theC. amongD. between53. — Tina looks especially pretty tonight.— Yes, she always looks her best in ____ of that color.A. dressB. a dressC. that dressD. the dress54. We elected Tom ____ monitor of our class for ____ second time.A. /; aB. the; aC. the; theD. a; a55. Papa made a ____ of agreement though his face clouded at the mention of Grandma’s name.A. pointB. signC. exampleD. mark56. Jack often turns to me for ____. Not long ago I gave him ____ on how to learn physics well.A. advices; a piece of good adviceB. advices; a good adviceC. advice; a good piece of adviceD. advice; advice57. The little girl asked her mother to buy her a nice pair of ____, so the mother went to some ____ stores.A. shoes; shoesB. shoes; shoeC. shoe; shoesD. shoe; shoe58. The boy didn’t have any ____ but to do as his father had told him.A. ideaB. wayC. chanceD. choice59. I cannot put up with ____. He is so naughty that I have to keep an eye on him so as to keep my things from being taken away.A. the boy of youB. that boy of yoursC. yours boyD. that of your boys60. — Have you visited the school?— No. We visited the Nanjing ____.A. Factory for SweaterB. Factory of SweaterC. Sweater FactoryD. Sweater’s Factory61. In the after-class activities, the students had plenty of ____ and gained a lot of practical ____.A. exercises; experiencesB. practices; experienceC. practice; experienceD. exercise; experiences62. Many old houses are being pulled down to make ____ for the apartment building.A. a spaceB. an areaC. roomD. ground63. If the boss wants to fi re me, he has to give me two months’ ____.A. informationB. adviceC. messageD. notice64. —Would you be so kind as to give me a ____? I’m too tired to walk any further.—I’m so sorry, but the car’s too crowded.A. seatB. liftC. driveD. place65. Pop music is loved by lots of young people, but it is not to everyone’s ____.A. smellB. tasteC. soundD. favor66. — Few children are as bright as he is, and also, he works very hard.—It’s no ____ he always wins first place in every examination.A. doubtB. wonderC. problemD. question67. It’s important for us to employ a word or a phrase according to the ____ in language studies.A. situationB. conditionC. translationD. expression68. I’m sorry I hurt your feelings; it wasn’t my ____.A. ideaB. thoughtC. mindD. intention69. — Carry your ____ with you, your money, jewelry, camera and so on.— OK. Thank you for reminding me.A. valuablesB. suitcasesC. giftsD. bags70. — Do you enjoy skating?— Yes, skating on real ice is ____.A. great funnyB. a great funC. of great funD. great funs71. — What would you like, sir?— ____, please.A. Two cups of teasB. Two teasC. Two cup of teaD. Two cups tea72. I’ve listened to Professor Smith’s talk about medicine, but I still haven’t got a good understanding of its key ____.A. notesB. informationC. wordsD. points73. She took a quick ____ at her watch and then started at once.A. viewB. glanceC. stareD. glare74. Enough of it! Nobody here thinks what you are saying should make any ____.A. excuseB. senseC. useD. value75. I am afraid I’ll turn to Mr. Black. It is ____.A. too difficult a problemB. a too difficult problemC. so difficult problemD. a so difficult problem76. — Do you know where your parents stay now?— Oh, at ____.A. Mr. FrankB. the Mr. FrankC. Mr. Frank’sD. the Frank’s77. On the wall is a photo of my mother who had black ____, but now she has some white ____.A. hairs; hairB. hair; hairsC. hair; hairD. hairs; hairs78. ____ children are still playing games outside.A. Two other littleB. Other two littleC. Little two otherD. Other little two79. She was very busy while staying at Macao. First she interviewed several employees in the branch office. In ____ days she paid a visit to some retired workers.A. the few nextB. a few nextC. the next fewD. next a few80. There are tall trees on ____ side of the streets, and ____ the trees are beautiful flowers.A. either; betweenB. both; betweenC. every; amongD. both; among81. The Smiths have three sons, one a baby, ____ twins of thirteen.A. anotherB. otherC. the otherD. the others82. ____ can do the job a lone, as the saying goes “Two heads are better than one.”A. NoneB. No oneC. AnyoneD. Everyone83. Nick’s father kept telling him that he should study harder, but ____ didn’t help.A. theseB. heC. whichD. it84. — Is ____ here?。

一、冠词冠词英语中常用a,an,the 来说明名词所表示的人或事物，这三个词常位于名词的前面，故称之为冠词。

冠词分为不定冠词a,an和定冠词the两种。

不定冠词表示泛指概念，定冠词表示特指概念。

冠词是虚词，是名词的一种标志，它不能脱离名词独立存在，不能单独作句子成分。

不定冠词a,an 与one同源，表不微弱的“—”的概念，但并不强调数量，用来表示不特定的人或事物。

a用于辅音音素开头的词前；an用于元音音素开头的词前。

a university 一所大学a European country一个欧洲国家an unfinished task 一项未完成的任务an honest person 一个诚实的人知识拓展university 中的u 读作[ju:],European 中的eu 读作[juə], 皆以半元音[j]开始，故其前应用a, 而不用an。

为了便于记忆，请熟记下面的句子：In a university,a European and a oneeyed man walked along a oneway road with a useful tool.This is a usual thing.在一所大学里，一个欧洲人和一个一只眼的人拿着一件有用的工具沿着一条单行道走着。

这是一件平常之事。

An hour ago,an honest man accepted an unusual gift and went to finish an honorable task.一小时以前，一个诚实的人接受了一件非同寻常的礼物，然后去完成一项光荣的任务。

2.表示“类别”指一类人或事物。

该用法并不表示具体的某一个人或事物，而是指一类人或事物，一般不译为“—”。

A doctor is a person who saves people's life.医生就是拯救人们生命的人。

第1页(共10页)A horse is a useful animal.马是一种有用的动物。

专题01 冠词、名词2022年高考真题1.（2022新高考I卷）Covering an area about three times __________ size of Yellowstone National Park, the GPNP will be one of the first national parks in the country.2．（2022全国甲卷）____________ friend of his, Wu Fan, volunteered to be his companion during the trip.3.（2022全国乙卷）To celebrate ___________ festival, a number of events took place at the Chinese Businessman Museum in Beijing on Thursday.4.（2022年浙江1月真题）Cobb, for her party, started to ask conference organizers who invited her to speak if she could do so remotely; about three-quarters of ___________ time, they agreed.5.（2022.6新高考1卷语法填空）The GPNP’s main goal is to improve connectivity between separate ___62___ (population)and homes of giant pandas, and _eventually (eventual)achieve a desired level of population in the wild.6.（2022全国乙卷）“As a main promoter of the International Tea Day, the birthplace of tea and the __largest_ (large) tea-producing country, China has a ___65___ (responsible)to work with other countries to promote the healthy development of the tea industry.2021年高考真题1.（2021.6新高考1卷2.（2021.6新高考1卷语法填空）The rolling sea of clouds you see once you are at the top will remind3.（2021.6全国甲卷语法填空）Supposedly you can do it in two hours, but we stopped at the different gatesand 9 (watchtower) to take pictures4.（2021.6全国乙卷5.（2021.6全国甲卷改错）Many students say they will talk to their friend or classmates because they're of the same age and can understand each other.6. （2021.1 浙江卷7.（2021.6全国甲卷语法填空）I t took us about 3 hours to go all 8 way around the Xi'an City Wall.8.（2021.6全国乙卷eco-friendly trip must meet the following principles:9.（2021.6 浙江卷meet the needs of their growing family.10. （2021.6 浙江卷语法填空）In 1844 they bought it for＄1,200 and some land from Charles11.（2021.6全国乙卷改错）I love doing housework. I always assist my parents in doing the dish after meals.12.（2021.6全国乙卷语法填空）I t is different from traditional tourism because it allows the traveler to become educated about the areas—both in terms of geographical conditions and cultural characteristics, and13.（2021.3 天津卷单选）Great improvements in public health have been brought aboutA. traditionsB. advancesC. pressuresD. examples2020年高考真题1.（2020·新课标I卷语法填空）The far side of the moon is of particular 64 (interesting) to scientists because it has a lot of deep craters (环形山), more so 65 the familiar near side.2.（2020·新课标I卷短文改错）Next I broke the eggs into a bowl and beat them quickly with chopstick.3.（2020·新课标II卷语法填空）Chinese New Year is a 61 (celebrate) marking the end of the winter season and the beginning of spring.4.（2020·新课标II卷短文改错）Luckily, I will go home in two weeks for summer vacations.5.（2020·江苏卷单项填空）The health security systems of many countries are undergoing considerable ______.A. reservationB. transformationC. distinctionD. submission。

高二英语冠词专题训练答案含解析一、单项选择冠词1．Summer is _______ good time for keeping fit, so you can join a sports club to help build your body in _______ summer of 2011.A．a; the B．/; / C．a; / D．/; the【答案】A【解析】考查冠词.上文春天是一个好时候,为泛指,用不定冠词.下文有限定词2011,为特指,用定冠词,故选A.2．---Of ____ two bags, which one do you think is better?--- _______ smaller one. I prefer how it looks.A．the; The B．the; A C．a; A D．a; The【答案】A【解析】根据题意这两个包裹，你认为哪一个比较好？这里是特指，较小的那个，也是特指，故都用定冠词the，选A。

3．It’s ________great surprise to find the poor little girl lives alone in _________countryside.A．a; the B．a; 不填C．不填; the D．不填；不填【答案】A【解析】根据题意可知巨大的惊讶是泛指，下文乡下是特指，故选A。

发现贫穷的小女孩自己一个人住在乡下是一个巨大的惊讶。

4．As ___ boy, he should learn how to behave like ____ real man sooner or later.A．a；the B．a; a C．/; the D．a；/【答案】B【解析】这题考查冠词的用法：第一空填a，泛指一个男孩，第二空填a，指“一个真正的男子汉”，句意是：做为一个男孩，迟早他应该学习怎么像个男子汉。

高二英语冠词试题1. My mother is English teacher in No. 2 Middle School.A．a B．theC．an D． /【答案】C【解析】考查冠词。

句意：我的妈妈是第二中学的一名英语老师。

第一次提到故用a/an，而紧跟着后面是English，是个元音开头的单词，故选C项。

考点 : 考查冠词2. With the successful launch of Tiangong-I, which was ______ breakthrough in space field, China's space dream took a step closer to ______ reality.A. a; /B. the; aC. a; the D the; /【答案】A【解析】句意：随着航空领域的一大突破天宫一号的成功发射，中国的太空梦朝现实更迈进了一步。

第一空填a，泛指这是一个突破，第二空不填，reality是抽象名词，选A。

【考点】考查冠词3. ---Have you got everything ready for _______ psychodrama(心理剧) competition?---Yes, all is in _____ place.A．the; the B．a; the C．a; /D．the; /【答案】D【解析】句意：你为心理剧的一切都做好了准备吗？--是的，一切都准备就绪。

第一空填the，特指说话双方都知道的心理剧，第二空不填，in place“在适当的位置，准备就绪”，选D。

【考点】考查冠词4.What I’ve just got for Christmas is ________ attractive ipad, ______ present I have been longing for.A．an; a B．an; the C．the; a D．the; the【答案】A【解析】句意：我圣诞节得到的是一个吸引人的ipad，一个我一直渴望的礼物。

知识全解冠词是一种虚词,无词义，也没有数和格的变化,不能独立使用,常常放在名词前,帮助说明这个名词的意义。

复习冠词须把握好以下三个原则:其一:单数可数名词用不定冠词a/an表示泛指;其二：复数可数名词及不可数名词表示泛指时，其前不加冠词;其三：无论可数名词还是不可数名词，表示特指其前都要加定冠词the.一、不定冠词的用法1。

不定冠词表示数量“一”，与one相近。

如：I will finish this work in a day。

我一天就要完成这项工作。

2.不定冠词用在首次提到的人或物之前，表示泛指。

如：There is a park on the corner of the street。

在这条街的拐角处有个公园。

3.不定冠词用在单数可数名词前，表示一类人或事物.如：A teacher must love his students。

教师必须热爱学生。

4.不定冠词表示单位,有“每一"之意.如：This cloth is 5 yuan a metre。

这种布料5元一米。

5.不定冠词用在物质名词、抽象名词之前，表示“一阵，异常，一种……"等等，也可以表示引起某种情绪的事，即抽象名词具体化使用。

如：Honesty is a virtue. 诚实是一种美德.It’s a pity that you missed this chance.你错过这次机会是件很遗憾的事。

6。

不定冠词用在人名前，表示说话者对此人不认识，相当于a certain,也可以指与某人名有类似性质的人或物。

如：A Mr Wang came to see you this morning。

今天早上一位王先生来找过你。

7。

用在有名词中的地名前，表示“一个……样的地方”。

如：She is now a dif ferent China from what she was twenty years ago。

8.用在带有修饰词的、表示三餐饭的名词之前，表示“一顿……的午（晚）饭”等.如:He likes to have a quick breakfast. 他喜欢吃快早餐。

高二英语冠词试题答案及解析1.South Korean rapper Psy’s hit song “Gangnam Style” made_______history on Dec. 20th as_______first ever video on YouTube to reach 1 billion views.A．the; the B．/; theC．a; a D．/; a【答案】B【解析】考查冠词。

句意: 2012 年 12 月 21 日, 韩国说唱歌手朴载相的热歌《江南style》作为在 YouTube 第一个点击量达到十亿的视频而创造了历史。

make history / go down in history为固定短语“载入史册；青史留名；创造历史”, 不需要加冠词；the first 第一。

【考点】冠词2. He has a great interest in ______ history, especially ______ history of the Tang Dynasty. A．the, the B．a；theC．不填； the D．the；不填【答案】C【解析】试题分析:句意：他对历史有着很大的兴趣，尤其是唐朝的历史。

历史是学科名，不用冠词；而第二个空特指唐朝历史，有定语限制，所以用定冠词the，答案选C。

【考点】考查冠词用法3. It makes _______ big difference to keep calm when giving _______ first aid.A．a, /B．/, the C．a, the D．/,a【答案】A【解析】考查冠词。

第一个空是固定短语make a big difference “起重要作用”。

第二个空也是固定短语first aid“急救”不用冠词。

句意：当实施急救时保持镇静很重要。

所以答案选A。

【考点】考查冠词4.It is known to all that ________ People’s Republic of China is ________ biggest developing country in the world.A．the; /B．/; the C．the; the D．/; /【答案】C【解析】考查冠词用法。

高二英语冠词专题训练答案及解析一、单项选择冠词1．Barack Obama, African America, was elected as 56th President of the United states. A．/，the B．/， / C．an ， the D．an ， /【答案】C【解析】前文一个美国黑人是泛指，且词首音素为元音，用不定冠词an，下文第56届美国总统是特指，用定冠词the，选C。

2．—Don’t you think Obama is _____ success?—Yes, I do. He is popular with _____ public in America.A．/ , a B．a, a C．the, a D．a, the【答案】D【解析】考察冠词用法，根据题意，你不认为奥巴马是一个成功人士吗？这里是泛指，用不定冠词。

是的，我这样认为。

他在美国深受大众欢迎。

这里的大众指的是美国的大众，为特指，故用定冠词。

所以选D。

3．When you finish reading the book, you will have __ better understanding of __ life.A．a, the B．the, a C．/, the D．a, /【答案】D【解析】试题分析：考查冠词。

本题第一空是固定词组have a better understanding of有更好的理解；第二空的名词Life是一个抽象名词，要单独使用，不需要冠词。

句意：当你读完这本书的时候，你会对生活有更深的理解。

故D正确。

考点：考查冠词点评：冠词的考查集中在the表示特指；a/an表示泛指。

另外要注意冠词的一些固定搭配中冠词的使用。

这题主要是抽象名词具体化的用法，除了honor还有success，failure，surprise，pleasure等名词也可以表示抽象名词具体化。

4．Little Brown lives in _____ A-shaped house near the town. It is easy for him to attend______ school.A．the; 不填B．不填; a C．an; a D．an; 不填【答案】D【解析】考查冠词。

（英语）高二必备英语冠词技巧全解及练习题(含答案)及解析一、单项选择冠词1．As he reached _____ back door, Jack saw _____ strange sight.A．the ; / B．a; the C．/; a D．the ; a【答案】D【解析】答案D[考查冠词在具体语境中的用法。

句意：当Jack走到前门时，看到了一个奇怪的景象。

the front door是特指Jack所走过去的门，a strange sight是泛指，相当于a certain sight，起初次介绍的作用。

因此选D。

]2．He wrote ______ song, which turned out to be rather popular with ______ young.A．the; the B．a; 不填C．a; the D．the; 不填【答案】C【解析】考查冠词，第一空是泛指一首歌，所以用不定冠词a，第二空考查：the +形容词表示一类人，句意：他写了一首歌在年轻人中变得非常流行。

所以选C。

3．Last year I had ______ narrow e scape from a car crash, and I don’t want to risk my life ______ second time.A．a; a B．the; a C．the; the D．a; the【答案】A【解析】试题分析：考查冠词。

句意：去年我从一场车祸中九死一生，我现在不想再一次使我的生命受到危险了。

A narrow escape九死一生，固定搭配，a second time 表示再一次，故选A 项。

考点：考查冠词【名师点睛】不定冠词的概念：冠词是置于名词前，说明各词所表示的人或事物的一种虚词，它不能离开名词而单独存在。

冠词有两种，一种是定冠词，一种是不定冠词。

定冠词是the，不定冠词有两种形式，一是a，另一是an。

专题01 冠词(2010﹒福建﹒T21)It’s good feeling for people to admire the Shanghai World Expo that gives them pleasure.A. 不填, aB. a, 不填C. the, aD. a, the(2010﹒山东﹒T 22)If we sit near _______ front of the bus, we’ll have _______ better view.A. 不填；theB. 不填；aC. the; aD. the；the(2010﹒江苏﹒T 21)The visitors here are greatly impressed by the fact that_______ people from all walks of life are working hard for_____ new Jiangsu.A.不填；aB. 不填；theC. the；aD. the；the(2010﹒辽宁﹒T 22)There are over 58, 000 rocky objects in ______ space, about 900 of which could fall down onto _______ earth.A. the; theB. 不填; theC. the; 不填D. a; the (2010﹒北京﹒T 35)First impressions are the most lasting. After all, you never get _____ second chance to make _____ first impression.A. a; theB. the; theC. a; aD. the; a(2010﹒重庆﹒T 27)Everything comes with price; there is no such thing as free lunch in the world.A. a, aB. the, /C. the, aD. a, /(2010﹒浙江﹒T 2)Many lifestyle patterns do such great harm to health that they actually speed up weakening of the human body.A. a; /B. /; theC. a; theD. /; /(2010﹒四川﹒T 2)In most countries, a university degree can give you flying start in life.A. the; aB. the; 不填C.不填; 不填D.不填; a(2011﹒全国卷I﹒T 33) It is generally accepted that boy must learn to stand up and fight like man.A.a; aB.a; theC.the; theD.a; 不填(2011﹒全国卷II﹒T 16) As he reached front door, Jack saw strange sight.A. the;不填B. a; theC.不填aD. the; a(2011﹒山东﹒T 21) Take your time—it’s just_____short distance from here to_____restaurant.A.不填；theB. a; theC. the; aD.不填；a (2011﹒浙江﹒T 2)Experts think that __________recently discovered painting may be ________ Picsso.A.the ;不填B. a ;theC. a ; 不填D. the; a(2011﹒四川﹒T 18)Dr.Peter Spence, headmaster of the school, told us, “fifth of pupils here go on to study at Oxford and Cambridge.”A. 不填；AB.不填；TheC.the; TheD.a; A(2011﹒重庆﹒T 26)In communication, a smile is usually __________ strong sign of a friendly and _______ open attitude.A. the, /B. a, anC. a, /D. the, an(2011﹒陕西﹒T 13)As is known to all, People’s Republic of China is biggest developing country in the world.A. the ;不填B. 不填；theC. the ；theD. 不填；不填(2011﹒江西﹒T 22)——It’s said John will be in a job paying over ＄60,000 _____ year.—— Right, he will also get paid by _____ week.A. the; theB. a; theC. the; aD.a; a(2012﹒全国卷II﹒T 7)He missed _____gold in the high jump, but will get _____second chance in the long jump.A. the, theB. /, aC. the, aD. a, /(2012﹒江西﹒T 21)The Smiths don’t usually stay at hotels, but last summer they spent a few days at a very nice hotel by ________ sea.A．/; a B．the; the C．/; the D．the; a(2012﹒浙江﹒T 2)The development of industry has been_______ gradual process throughout _______ human existence, from stone tools to modern technology.A. 不填, theB. the; aC. a; 不填D. a; a(2012﹒安徽﹒T 22)Carl is studying food science at college and hopes to open up meat processing factory of his own one day.A./ ；aB. / ；theC. the ; aD. the ; the(2012﹒重庆﹒T 24)Sam has been appointed _______ manager of the engineering department to take ____ place of George.A. /,/B. the,/ C the the D./, the(2012﹒辽宁﹒T 21)I woke up with bad headache, yet by evening the pain had gone.A. the; theB. the; anC. a; theD. a; an (2012﹒全国新课程﹒T 22) Sarah looked at finished painting with satisfaction.A.不填；aB. a; theC. the; 不填D. the; a(2012﹒全国新课程﹒T 29) Being able to afford _______ drink would be ______ comfort in those tough times.A. the; theB. a; aC. a; 不填D. 不填；a (2013﹒福建﹒T 21) The “Chinese Dream” is ______ dream to improve people’s well-being and ______ dream of harmony, peace and development.A. the; aB. a; aC. a; theD. the; the (2013﹒江西﹒T32) Animals are obviously ______ lower form of life than _______ man.A. a ; /B. the ; theC. a ; theD. / ; /(2013﹒山东﹒T 22) It was ______ cold winter night and the moon was shining brightly across ____ night sky.A. 不填；aB. a; theC. the; aD. the; 不填(2013﹒陕西﹒T 19) Marco Polo is said to have sailed on Pacific Ocean on his way to Java in I thirteenth century.A. the; aB. a; /C. /; theD. the; the (2013﹒新课标II﹒T 13) Four and half hours of discussion took us up to midnight, and break for cheese, chocolate and tea with sugar.A. a; aB. the; theC.不填；theD. a;不填(2013﹒新课标I﹒T 31) India attained independence in 1947, after long struggle.A.不填;aB. the; aC. an;不填D. an; the (2013﹒浙江﹒T 15) People develop ______ preference for a particular style of learning at ______ early age and these preferences affect learning.A. a; anB. a; 不填C. 不填; theD. the; an (2013﹒重庆﹒T32) The parents were shocked by news that their son needed operation on his knee.A. a;/B. the; /C. the; anD. a; an (2014﹒江西﹒T 22) They chose Tom to be ________ captain of the team, because they knew he was ________ smart leader.A. a; theB. the; theC. the; aD. a; a(2014﹒陕西﹒T 19) ________ village where I was born has grown into ________ town.A. The; aB. A; theC. The; theD. A; a(2014﹒天津﹒T 8) Life is like ________ ocean: only ________ strong-willed can reach the other shore.A. an; theB. the; aC. the ；/D. / ; a(2014﹒浙江﹒T 2) The paper is due next month，and I am working seven days ________ week，often long into ________ night.A．a; the B．the; 不填C．a; a D．不填; the (2014﹒重庆﹒T 6) I can't tell you ________ way to the Wilsons' because we don't have ________ Wilson here in the village.A. the; aB. a; /C. a; theD. the; /(2015﹒陕西﹒T27) more learned a man is, more modest be usually become.A. The ; theB. A; aC. The ; aD. A; the(2015﹒四川﹒T5).Brian is gifted in writing music; he is very likely to be Beethoven.A. aB. anC. theD.不填(2015﹒浙江﹒T2) Jane’s grandmother had wanted to write children’s book for many years, but one thing or another always got in way.A. a;不填B. the;theC. 不填;theD. a;the(2015﹒重庆﹒T3) I just heard____ bank where Dora works was robbed by____ gunman wearing a mask.A.the; /B. a; /C. the; aD. a; the(2016﹒浙江﹒T2)______prize for the winner of the competition is______two-week holiday in Paris.A. The ; 不填B. A ; 不填C. A ; theD. The ; a(2019﹒浙江﹒T57)Everybody wears___57___ same style of clothes.(2019﹒新课标I卷﹒T69)Of ___69___ nineteen recognized polar bear subpopulations, three are declining, six are (be) stable, one is increasing, and nine lack enough data.。

高二英语冠词用法【本讲主要内容】冠词用法【知识总结归纳】（一）冠词分为不定冠词（a / an ）和定冠词（the），冠词用在名词前面。

句子中根据名词的语义来确定冠词的使用。

在某些情况下，有些名词前只能用定冠词the，有些名词前不加任何冠词。

（二）不定冠词的用法：不定冠词a / an 用在单数可数名词前面，意思是“一个”。

当名词的第一个音节发音为元音时，用an。

1. 第一次提到某个人或事物时，起介绍作用，或表示泛指。

Christmas is actually a family festival.Harry has been admitted to a western university.Phil can’t go to the movies tonight because he has to write an essay.2. 表示某一类人或物，用于类指。

The whale is a mammal, not a fish.Birds of a feather flock together.Two of a trade can never agree.3. 表示事物的单位，意思是：“每”He often drives at eighty miles an hour.The temperature’s rising by about three degrees an hour.Those shoes cost only four dollars a pair.4. 用在某些抽象名词前面，使这个抽象名词具体化，指：具体的人或一件事。

The little girl is a joy to her parents.She got a big surprise when she saw all those books on the floor.He has a good knowledge of mathematics.Let’s be a warning to you.5. 用在某些物质名词前面，使其成为具体的“一种”，“一顿”，“一阵”，“一份”等。

综合测试02 名词、冠词、介词、代词、形容词、副词、代词题型一名词单句语法填空1．While planning my vacation, the travel agent provided me with valuable (assist) in choosing the perfect destination.2．When we want validation for our decisions we often turn to friends for advice and (approve). 3．My father is responsible, who always places great (emphasize) on the importance of family life and ties.（4．Recycling the waste from our increased (consume) is better than burning it.5．Although parks of all sizes and types exist at any level, the national parks, in particular, tend to catch our attention because of their large size and variety. They are (treasure) of American heritage (遗产). 6．It is believed that (today) children and teenagers are consuming three times the recommended level of sugar, putting them at a higher risk of the disease.7．Owning a lovable, cuddly pet can unquestionably make one’s home life better and apparently, it can affect pet (owner) professional lives as well.8．The return capsule of (China) Chang’e 5 probe touched down on its preset landing site in Siziwang banner of the Inner Mongolia autonomous region at 1:59 am on Thursday, bringing back the country’s first samples collected from the moon, according to the China National Space Administration (CNSA).9．It is believed that nothing can stand in your way to success if you never stop trying and show great (persevere).10．The reasons for the success and (popular) of British comedy in particular, are interesting to explore.题型二冠词单句语法填空11．The stores in the Chinatown offer unique range of souvenirs, goods, and clothing. 12．Despite the storm, only handful of trees were knocked down in the neighborhood.13．The present-day Analects is based on the Lu version compiled during the Han Dynasty and embodies, Confucian concept of ren or humanity or benevolence.14．Of all the subjects, I like history best because it gives us useful knowledge of things in the past. 15．Zhu Dejun’s first job after graduating from college was as road design engineer in his hometown. 16．outside of buildings was decorated with stone lions, screen walls, decorative columns, as well as flowers.17．Many of us might not give second thought to dropping a small piece of litter.18．Peru is country on the Pacific coast of South America.19．Hua Weiguang, retired teacher from Tongliao, Inner Mongolia Autonomous Region, is a photography enthusiast.20．In Song Dynasty, an instrument was also invented to indicate direction, called the compass fish, which floated on water.题型三介词单句语法填空21．In order to get our knowledge enriched, we should actively participate in some activities that are related reading.22．A new secretary will be hired to relieve us some of the paperwork.23．The two companies are competing a bigger market at the moment.24．A lot of that will determine whether we have integrity of elections.25．Put the medicine children's reach in case it's taken by mistake.26．Seeing the flight to New York take off, the girl was totally despair.27．The recipe happiness that Sang Lan comes up with is to have appreciation for good things in life.28．In (real), different students have different learning habits, which I think influence their learning efficiency greatly.29．After careful consideration, he chose to withdraw the competition.30．Our school library has books of all kinds that range from classic novels latest magazines. 31．The audience supplied a lot of information us, which affected us deeply.32．Nina has run marathons in 32 countries. All of her runs have a guiding purpose: to call attentionglobal water issues.33．“Have a good day” is a wonderful phrase, reminding us, effect, to enjoy the moment.34．She gladly gave up her part-time job to devote herself entirely her art.35．He added that there are many risks involved when Al is capable exercising its own judgement. 36．Sichuan Cuisine, Hunan Cuisine does not typically include the numbing sensation of Sichuan peppercorns.37．I think that it comes down the fact that people do feel very dependent on their automobiles. 38．He was accused stealing bikes at school, which was beyond our expectations.39．They are arguing with their classmates about the solution the problem.40．The No. 2 shipwreck dates the rule of Emperor Hongzhi and measures about 21 meters long and 8 meters wide.41．We start with small talk, a polite conversation about something traffic or weather.42．He goes to the library every day when he is not well.43．Much as he likes her, he does get annoyed her sometimes.44．Jack has a passion photography and his photos are always of high quality.45．The man was spied by the police, because he was a suspect.46．Lu Gandarias, who’s now thirteen, went blind suddenly age seven.47．I happen to know that Tang’s play The Peony Pavilion (《牡丹亭》) is similar in some ways Romeo and Juliet.48．Though he has been power for two years, he hasn't found an effective solution to the country's economic problems.49．“Hopefully, I can be of some help in inspiring more young people to find their lifelong passioncultural heritage preservation,” said Song.50．Gui then pursued postdoctoral research overseas and published about 20 SCI academic papers in top international journals. After returning to China, he received Beihang’s invitation to teach an associate professor.题型四形容词单句语法填空51．With food our biggest (week) household expense，Susanna and Matt spend time with a different family.52．It has been confirmed that the (injure) in the car crash were sent to the nearest hospital at once. （53．The (wound)are being taken good care of in the hospital.54．The study showed a deep fear among the (elder) of being abandoned to the care of strangers. 55．“Sleep is of the (die).” “Sleep is a criminal waste of time.” That’s not me talking. （56．The novel, for the most part, was looked upon as silly or (moral).57．The human tongue can detect five distinct tastes: sweet, (salt), sour, bitter and umami.58．The Ten Wings made Zhouyi much (easy) for ordinary people at the time to understand and are still used today.59．As we all know, the older one gets, the (precious) time becomes to him.60. Still consider the CBA China’s 60 (popular) basketball league? Think again! （题型五副词单句语法填空61．Taking a walk outdoors, I never cease to be (pleasant) surprised to encounter far more birds than I thought.62．The Shenzhou-13 return capsule, carrying the three Chinese astronauts, Zhai Zhigang, Wang Yaping and Ye Guangfu, (safe) landed back on Earth on Saturday morning after a record breaking six-month mission in space.63．Zhaoling Tomb is a (full) restored tomb according to the old site of the Ming Dynasty, which is magnificent with high pines and glorious halls.64．The museum was (origin) constructed to house the vast art collection belonging to oil tycoon J. Paul Getty.65．Divided into five sections, the long-running show is aimed to offer art collectors and the general public alike an opportunity to learn about China’s modern, contemporary print art and (far) explore the market value of prints, according to Liu Zehui, the person in charge of the show.66．The system sends messages to people (little) than two seconds after the earthquake happens. 67．The world needs a mixture of personalities. Success is not just about who can shout the (loud). The world needs quieter people, too. （68．Some senior high school students looked (curious) at the exchange student.69．It was also (extraordinary) popular among many royal families of the Qing Dynasty.70．The West Lake is (definite) a place people can never miss when visiting Hangzhou.题型六代词单句语法填空71．Swimming is my favorite sport. There is like swimming as a means of keeping fit.72．He seemed to make his honour to hold back the tears.73．It’s similar to Jupiter, a giant gas planet in (we) solar system.74．For China, everything these countries gain from (it) help is a gain for the world. (所给词的的适当形式填空)75．It is part of a drive by the Department for Education to address the rise in students who report(they) mental health issues at university.76．When he gets anxious, he often breathes deeply to calm (he) down.77．You’ll spend one day travelling by boat to (you) accommodation in the middle of the forest. 78．However, he believes that the practice with fountain pens helps students to focus, to write faster, and they can feel proud of (they).79．You’d better behave (you) when taking exams.80．And now we find (we) in the great new age of technology.题型七名校语篇填空Passage1（浙江学军中学2024年高三模拟试题）阅读下面短文，在空白处填入1个适当的单词或括号内单词的正确形式。

2023年高二英语语法配套练习：01 冠词一、语法填空(共30题；共60分)1．（2分）number of cars on expressways is rising because of free passage during holidays. 2．（2分）Could you do me favour and pick up Sam from school today?3．（2分）This jacket is real bargain at such a low price.4．（2分）He gave me smile.5．（2分）Lily was born in early 2000s and is about twenty years old now.6．（2分）The little boy has gift for piano and he practices it with great effort.7．（2分）Terrorism is threat to the whole country.8．（2分）Victoria worked hard and set example to her classmates. （用适当的词填空）9．（2分）My sister has good command of English because she has studied abroad for three years.10．（2分）The system wastes large amount of water.11．（2分）Pandas are endangered species, which now is well protected in China. 12．（2分）We can remember up to 200 pieces of information in second at the age of 25. 13．（2分）I must warn you that my patience is almost coming to end．（根据句意填空）14．（2分）The girl tried to escape after the theft, but having found that, the shop assistant seized her by hair.15．（2分）He didn't come to school today because he had frog in his throat.16．（2分）As member of the school volleyball team, I wasn't selected for the end-of-year competition. (用适当的词填空)17．（2分）We should bring end to the project as soon as we can.18．（2分）Learning grammar is big headache for me.19．（2分）When she finally arrived at the lecture hall, the lecture had already come to end. （用适当的词填空）20．（2分）The team made great breakthrough in developing a vaccine to conquer the disease which had taken thousands of people's lives. （用适当的词填空）21．（2分）Babies are not born knowing the basic fact of universe.22．（2分）Although he's only amateur, he's a first-class master.23．（2分）Can you give instance of the boy's ability?24．（2分）Being able to afford a drink would be comfort in those tough times.25．（2分）John ran away from home after quarrel with his wife.26．（2分）We should take the advice of the woman who is expert in foreign language. 27．（2分）instant I saw him, I knew he was the man the police were looking for.28．（2分）He put me on spot a bit because he invited me right in front of his mum and I didn't particularly want to go.29．（2分）I just felt I had been failure in my personal life.30．（2分）The result of the election came as shock to us all.答案解析部分1．【答案】The【解析】【分析】句意：由于假日期间的自由通行，高速公路上的汽车数量正在增加。

2023年高二英语语法配套练习：05 冠词一、单选题(共20题；共40分)1．（2分）We should protect wild animals from being harmed and live in _______harmony with them.A．/B．a C．the D．an2．（2分）At the end of ______ 16th century, about five to seven million people speak ______ English.A．the; /B．the; the C．/; the D．/; /3．（2分）—I went fishing with my good friend yesterday.—Wow, it was really _________ enjoyable day.A．a B．an C．the D．/4．（2分）We each took a cup of ________ tea after having ________ wonderful supper at my aunt's yesterday.A．a; /B．/; /C．the; the D．/; a5．（2分）Jack was playing ________ guitar while I was playing ________ soccer.A．a; /B．the; /C．/; the D．/; a6．（2分）The secretary arranged ________ convenient time and place for the applicants to have an interview.A．an B．a C．the D．/7．（2分）Some researchers point out that _________ daydreaming is _________ means of relaxation.A．the; a B．/; the C．a; the D．/; a8．（2分）It's not only ______ great responsibility but ______ great honor for a city to be chosen to host the Olympic Games.A．/; a B．/;/C．the; the D．a; a9．（2分）The Chinese women's volleyball team holds very special place in nation's heart.A．a; /B．the; the C．a; the D．the; a10．（2分）The woman who used to be in _______ charge of a big company is in _______ charge of a nurse at present.A．the; 不填B．the; the C．不填; 不填D．不填; the11．（2分）I would wear ______ unique green hair when I was in my twenties.A．a B．an C．the D．/12．（2分）He missed ______ gold in the high jump, but will get _____ second chance in the long jump.A．the; the B．/; a C．the; a D．a; /13．（2分）Believe it or not, everything comes with_____ price; there is no such_____ thing as free lunch.A．a; a B．the; /C．/; /D．a; /14．（2分）They waited for _______ unexpected to happen.A．an B．a C．the D．/15．（2分）— _______ expensive mobile phone it is！—Yes, but I think _______ style is quite fashionable.A．What a; a B．How; the C．What an; the D．How an; the 16．（2分）Don't take it seriously, Alice. I wasn't making fun of you — it's nothing butjoke.A．/; the B．the; the C．the; a D．/; a17．（2分）China, ______ largest developing country in the world, has made great progress in ______ area of space exploration.A．a; an B．the; the C．the; an D．a; the18．（2分）They buried _____ dead and took _____ wounded people to the hospital at once.A．a; the B．the; the C．the; a D．不填; the19．（2分）Later ________ word came that Dolly ________ sheep had become seriously ill.A．a; a B．the; the C．/; the D．/; /20．（2分）— Hello, could I speak to Mr. Smith?— Sorry, wrong number. There isn't______ Mr. Smith here.A．不填B．a C．the D．one二、语法填空(共20题；共40分)21．（2分）Having a bad boss is more than annoyance.22．（2分）Features are written in individual style, with extra detail and background. 23．（2分）It has been found that parents ' words and deeds have effect on their children. 24．（2分）He put me on spot a bit because he invited me right in front of his mum and I didn't particularly want to go.25．（2分）She persevered until Harry Potter became global success. （用适当的词填空）26．（2分）Everyone has role in advancing purpose.27．（2分）He bought set of furniture, simple and beautiful.28．（2分）The headmaster informed Mr Li that he would attend a meeting next day. 29．（2分）Lily was born in early 2000s and is about twenty years old now.30．（2分）This is automatic reply, (confirm) that your e-mail has been received.31．（2分）(release) on February 5, 2019, The Wandering Earth soon becamegreat hit.32．（2分）Unless there is evidence to contrary, we have no choice but(believe) what the chef has said.33．（2分）When the lady (dress) in an elegant dress came back, I found bunch of flowers in her hand.34．（2分）Your daughter has good grasp of English.35．（2分）He tricked us into believing that the road twisted uphill all way.36．（2分）As far as I am concerned, this will be unique adventure.37．（2分）As result of the homesickness, the little girl quit her job.38．（2分）Those extra costs must be kept to minimum for we must strengthen the pay-off control.39．（2分）My grandfather, an old soldier, had narrow escape in that fierce battle.40．（2分）You'd sound a lot more polite if you make request in the form of a question.三、改错题(共1题；共20分)41．（20分）18、单句改错每处错误仅涉及一个单词的增加，删除或修改。

高二英语暑假专题冠词知识精讲人教实验版一. 本周教学内容：暑假专题：冠词［学习过程］一、不定冠词的用法冠词是虚词，本身不能单独使用，也没有词义，它用在名词的前面，帮助指明名词的含义。

英语中的冠词有三种，一种是定冠词〔the Definite Article〕，另一种是不定冠词〔the Indefinite Article〕，还有一种是零冠词〔Zero Article〕。

不定冠词a (an)与数词one 同源，是“一个〞的意思。

a用于辅音音素前，一般读作[e]，而an那么用于元音音素前，一般读做[en]。

1〕表示“一个〞，意为one；指某人或者某物，意为a certain。

A Mr. Ling is waiting for you.2〕代表一类人或者物。

A knife is a tool for cutting with.Mr. Smith is an engineer.3〕词组或者成语。

a little / a few / a lot / a type of / a pile / a great many / many a / as a rule / in a hurry / in a minute / in a word / in a short while / after a while / have a cold / have a try / keep an eye on / all of a sudden二、定冠词的用法定冠词the与指示代词this，that同源，有“那〔这〕个〞的意思，但较弱，可以和一个名词连用，来表示某个或者某些特定的人或者东西。

1〕特指双方都明白的人或者物：Take the medicine. 把药吃了。

2〕上文提到过的人或者事：He bought a house. I’ve been to the house.他买了幢房子。

我去过那幢房子。

3〕指世上独一无二的事物：the sun， the sky， the moon， the earth4〕单数名词连用表示一类事物，如：the dollar 美元; the fox 狐狸；或者与形容词或者分词连用，表示一类人：the rich 富人; the living 生者。

高二年级英语课程语法专题（冠词）史海蓉【题目构成与意图】：尽管冠词在整个英语的语法体系的地位没有非谓语动词或复合句那么重要，但是它的影响却无处不在。

学好冠词的关键在于合理的归纳于缜密的记忆。

以下就高中阶段常用的冠词用法予以归纳，以便同学们构建更加合理的语法体系。

【建议完成时间】: 1小时【知识/概念梳理】：1．抽象名词具体化：表示一个具体的人或物时，可以使用不定冠词a/an;There is no doubt that the coming Olympic Games will be a great success.2．某些习语中常用不定冠词a/an的有：as a whole; as a rule; as a result; as a matter of fact; at a loss; all of a sudden; in a word;in a word; in a way; once in a while; lend a hand; keep an eye on;turn a deaf ear to;make a living; make a promise; die a glorious death; dream a bad dream;get in a word;have a word with sb.; make an effort; make it a rule; pay a visit to;take an interest in;come to an end; make a fortune;3．定冠词要用在江河、山脉、海洋、群岛名称前：the Yangze River; the Thames; the Atlantic Ocean; the Alps;the Gulf of Mexico;4．用于由普通名词构成的专有名词前：the Communist Party of China;the Hope Project; the Youth League;5．某些习语中常用定冠词the 的有：by the hour; by the way; on the phone; go to the cinema; in the day;in the least;at the moment; on the whole; on the air; on the contrary; in the long run;on the other hand;out of the question; on the decrease; to the point; in the way; for the time being; at the sight of;hit sb. in the face; take sb. by the hand; make the bed; to tell the truth;keep the peace; pick up the pieces; put the blame on;6．表示职位、头衔的名词在句中作表语、同位语、补足语时不用冠词，但作主语或宾语时则可以使用冠词。

One, the noun explanation1. The cDNA and cccDNA: cDNA was produced by reverse transcriptase mRNA synthetic double-stranded DNA; CccDNA is free from the chromosome plasmid double-stranded closed circular DNA.2. Standard folding units: protein secondary structure unit of alpha helix and beta - fold through a variety of connecting peptide can form the special geometry structure, determine the folding type often referred to as a super second structure. Almost all of the tertiary structure can use these folding type, and their combination to be described, so called standard folding unit again.3. CAP: cyclic adenosine monophosphate (cAMP) receptor proteins CRP (cAMP receptor protein), cAMP and CRP complexes formed by the combination of said activated protein CAP (cAMP activated protein)4. Palindromic sequence: a period of DNA fragments of the reverse complementary sequence, is often a restriction enzyme site.5. MicRNA: complementary RNA interference or called antisense RNA, and complementary mRNA sequence, inhibits the translation of mRNA.6. Nuclear enzymes: has the catalytic activity of RNA, the RNA splicing processing process of catalytic effect.7. Die body: there were some three-dimensional shape in the protein spatial structure and topology structure are similar to the local area8. Signal peptide: in the process of protein synthesis n-terminal 15 ~ 36 amino acid residues of peptides, guide the proteins across the membrane.9. Attenuator: in the control area and a may terminate the transcription of structural genes between the nucleotide sequence.10. The magic spot: when the bacteria grow in the process, when you meet the overall lack of amino acid bacteria will have an emergency response, stop all gene expression. Produce the emergency response signals are four guanosine phosphate (ppGpp) and guanosine 5 phosphate (pppGpp). PpGpp and pppGpp role is not just one or a few operon, but affect a large number of, so that they are super regulator or dark spots.11. The upstream promoter element: refers to the activity of the promoter plays a regulatory role of DNA sequences, TATA - 10 area, TGACA - 35 area and enhancer, attenuator, etc.12. : the DNA probe is a mark of a known sequence of DNA, to detect unknown sequence, screening purposes, etc are widely used.13. The SD sequence: is the ribosome combined with mRNA sequence, regulation of translation.14. Monoclonal antibodies: only for single antigenic determinant antibody.15. Mr Plasmid: is a kind of exogenous DNA after artificial building of the carrier, keep at the ends of the phage COS area, connected with plasmid.16. The blue - white spot screening: including LacZ gene (beta galactose glucoside enzyme) encoding this enzyme can decompose the chromogenic substrate X - gal (5 - bromine - chlorine - 4-3 - indole - beta - D - galactose glucoside) produce blue, so that the strain to blue. After the exogenous DNA insertion, LacZ gene cannot express, with a white strains, screening of recombinant bacteria. Call it blue - white spot screening.17. Cis function components: in a particular sequence of bases in DNA and to regulate the expression of genes of genetic components.18. Klenow enzymes: segments of DNA polymerase I big, just from the DNA polymerase Iholoenzyme to enzyme activity except for 5 '3'19. Anchored PCR: used for the purpose of DNA amplification known end sequence. In unknown sequence at one end and the tail of a polymer to dG, then respectively with the sequence of dC polymer and known as a primer for PCR amplification.20. Fusion protein: eukaryotic protein genes and foreign genes connection, at the same time, translation of the original gene protein expression together with foreign proteins composed of protein.Second, fill in the empty1. The physical map of DNA is the molecules of DNA restriction enzyme digestion (to) the order of the fragment.Shear is divided into 2. RNA enzyme (self), 1 (catalytic) two types.3. There are three kinds of initiation in prokaryotes (IF 1), (IF - 2) and (IF - 3).Need 4. Protein across a cell membrane (signal peptide) guidance, the role of protein partner is auxiliary peptides (natural conformation of proteins fold into).5. The promoter of the core promoter element usually can be divided into two kinds: (components) and (upstream promoter element).6. Molecular biology research content mainly includes structure (molecular biology), () gene regulation and expression, (recombinant DNA technology) three parts.7. Prove that DNA is the genetic material of two key experiment is (pneumococcal infections in mice), e. coli (T2 phage infection) the two experiments, the main argument is: (biological absorption of exogenous DNA evidence changed its genetic potential).8. The difference between with mRNA is transcribed there are two main: (transcribed in the process of transformation for mRNA after splicing,),(mRNA in the 5 'end is coupled with a m7pGppp hat, one more in the end of the mRNA3' poly a (polyA) tail).9. Protein subunits form has the advantage of more (the basis for the use of DNA) is a kind of economy method, (can reduce the random error in the process of protein synthesis effect on the activity of protein), activity (can be very effective and quickly open and closed).10. The main content of the protein folding mechanism first nucleation theory including (nucleation), structure (enrich), (last).11. Galactose on bacteria has a dual function; On the one hand, can be used as a carbon source for cell growth); On the other hand, it is the cell wall component). So I need a is not dependent on the cAMP - CRP promoter background level of permanent type synthesis of S2; Also need a depends on the cAMP - CRP promoter S1 adjusted for high level synthesis. Have a G (S2) transcription start, without G transcription start (S1).12. Recombinant DNA technology, also known as (gene cloning), or (molecular cloning). Ultimate aim is the genetic information of an organism (DNA into another organism). Typical of the recombinant DNA experiment usually includes the following steps:(1) the purpose of the extraction of donor biological gene (or exogenous gene), enzyme have received another (cloning vector) on the DNA molecule, forming a new recombinant DNA molecules.(2) the recombinant DNA molecules into the receptor cells and copy kept in receptor cells, this process is called transformation.(3) for those absorbed recombinant DNA screening and identification of receptor cells.(4) to mass culture cells containing recombinant DNA testing foreign gene is expressed.13, there are two kinds of plasmid replication types: get the strict control of the host cell protein synthesis are called (plasmid was type), is not subject to the strict control of the host cell protein synthesis is called (relaxed plasmid).14. The PCR reaction system to have the following conditions:A, isolated gene sequence mutual complement each chain end of DNA primers (about 20 bases). B, has a thermal stability of enzyme, such as: TagDNA polymerase.C, dNTPD, as the purpose of the template DNA sequence15. The basic PCR reaction process including: (degeneration), (annealing), three phases (extension).16, the basic process of transgenic animals usually include:(1) the cloning of exogenous gene into a fertilized ovum or embryonic stem cell nucleus;(2) after inoculation of fertilized ovum or embryonic stem cells transplanted into the female uterus;(3) complete the embryonic development, growth and for posterity with exogenous genes;(4) use of these can produce foreign proteins of animals as breeding stock, foster new homozygous.17. The production of hybridoma cell line is by the spleen (B) cells and (SMM) hybrid generation, because can make use of hypoxanthine (spleen cells), (bone cells) provide function of cell division, so can grow in the HAT medium.18. With the deepening of the study is called the first generation of antibodies (polyclonal antibody), the second generation (monoclonal antibody), the third generation of genetic engineering antibody ().19. The current of insect virus gene engineering are mainly concentrated in baculovirus, in introducing (exogenous toxic protein gene); (disrupt the normal life cycle of insect gene); (by modifying the virus gene).20. Mammals RNA polymerase Ⅱpromoter of common components, GC, TA TA CAAT trans action of the protein factor TFIID (), (SP - 1) and (NF1 / CTF).21. The RNA polymerase Ⅱbasic transcription factors have, TF, TF ⅡⅡ- A - B, TFII - D, TF Ⅱ- E they order is: the combination of (D, A, B, E). The TFII - D function is (with the TATA box).22. Combine with DNA transcription factor mostly in the form of a dimer, transcription factors and the function of the DNA binding domain has the following several kinds of common (screw - corner - screw), (zinc die body), (alkaline leucine zipper die bodies).23. The restriction enzyme cutting way of three types are respectively (the axis of symmetry 5 'side cut produce 5' sticky side), and (in the axis of symmetry 3 'side cut produce 3' sticky end (at the axis of symmetry cutting flat section).24. The plasmid DNA has three different configuration, respectively is: (SC), paper (oc) and configuration (L). The front in the electrophoresis is configuration (SC).25. The exogenous gene expression system, the main (e. coli), (yeast), (insects) and mammalian cells (table).26. The transgenic animals commonly used methods are: (retrovirus infections), micro injection (DNA) and method (embryonic stem cells).Third, short answer1. Name at least 5 kinds of RNA function respectively?Transport: RNA tRNA amino acidsRibosomal RNA (rRNA up into a ribosomeMRNA messenger RNA template protein synthesisIs a nuclear RNA is transcribed mature mRNA precursorsSmall nuclear RNA splicing snRNA participation is transcribedLittle cytoplasm RNA scRNA endoplasmic reticulum protein localization / 7 sl - RNA synthesis of signal identification of the componentsAntisense RNA anRNA/micRNA for regulating gene expressionEnzyme activity of RNA ribozymes Ribozyme RNA2. The promoter of prokaryotes and eukaryotes major difference?prokaryotesTTGACA TATAAT -- -- -- -- -- -- initiation site- 35-10eukaryotesEnhancer - GC - CAAT -- TATAA - 5 MGPP - starting sites- 110-70-253. The artificial construction of natural plasmid in what respect?Usually exist defects, natural plasmid and therefore not suitable for used as the carrier of genetic engineering, and must be built to transform:A, add the appropriate choice of marker genes, such as two or more, is easy to be used as a choice, usually antibiotic gene.B, increase or reduce the appropriate enzyme loci, facilitate the restructuring.C, shorten the length, cut off the unnecessary fragments, improve the efficiency of import, increase the loading capacity.D, change replicators, getting tightness is flabby, less copy multiple copies.E, according to the special requirements of genetic engineering to add special genetic components 4. Illustrate the method of the differential screening organization specific cDNA?Preparation of two kinds of cell groups, the purpose gene expressed in one of the cells or high expression of free expression or another type of cell in low expression, and then find the purpose gene through hybridization contrast.For example, in the process of tumorigenesis and development, the tumor cells will show different mRNA expression level, and normal cells, therefore, can be through differential hybridization screen genes associated with cancer. Can also use the induction method, induce gene expression.5. Production of hybridoma cell line and selection?Spleen + myeloma cells, B cells addition polymerization ethylene glycol (PEG) promote cell fusion, HAT medium culture (including hypoxanthine, aminopterin, T) grow spleen B - myeloma cells continue to expand training.Cell fusion content include:Spleen, spleen cells: can grow, spleen cells in vitro.Bone, bone fusion cells: not using hypoxanthine, but by the second way to diameter using folic acid reductase synthetic purine. Aminopterin has inhibitory effect on folic acid reductase, so youcan't grow.Bone - spleen cells: growth in HA T, and spleen cells can use hypoxanthine, bone cells provide the function of cell division.6, using the method of double deoxidizing terminal end (Sanger method) principle and method of determination of DNA primary structure?Principle is to use nucleotide chain termination agent - 2, and 3, double DNA nucleotides in the DNA of the termination. Due to its lack of forming 3/5 / dihydrogen phosphate ester bond need 3 - OH, once the participation into the strands of DNA, the DNA chain would not be extended further. According to the principle of base pairing, whenever DNA polymerase need dNMP participation to normal extension of DNA, there are two possibilities, one is involved in ddNTP, this led to the termination of DNA nucleotides chain extension; 2 it is involved in dNTP, make DNA can still continue to extend, until the next ddNTP refs. According to this method, we can get a set with ddNTP at the end of the different length of DNA fragments.Method is divided into four groups of ddAMP respectively, ddGMP, ddCMP, ddTMP reaction after polyacrylamide gel electrophoresis in swimming can read the DNA sequence.7, activating protein (CAP) is regulation function of transcription?Cyclic adenosine monophosphate (cAMP) receptor protein CRP (cAMP receptor protein), cAMP and CRP complexes formed by the combination of said CAP activated protein (cAMPactivated protein). When the growth of e. coli in the lack of glucose medium, synthesis increase CAP, CAP with active lactose (Lac) and so on the function of the promoter. Some rely on CRP promoter lacks general promoter of typical - 35 (TTGACA) of sequence characteristics. So the RNA polymerase and its combined hard.The existence of the CAP (function) : can significantly increase the enzyme combined with the promoter constant. Mainly the following two aspects:1 CAP by changing the conformation of the promoter and the interaction with enzymes help correct orientation of enzyme, so as to combine with - 10 area, have the effect of replace - 35 area function.(2) the CAP can also inhibit the combination of RNA polymerase and DNA to other sites, so as to improve its specific promoter combined with probability.8 what steps, typical of the recombinant DNA experiment usually include?A, the purpose of the extraction of donor biological gene (or exogenous gene), enzyme have received another (cloning vector) on the DNA molecule, forming a new recombinant DNA molecules.B, the recombinant DNA molecule into receptor cells and copy kept in receptor cells, this process is called transformation.C, for those who absorb the screening and identification of recombinant DNA receptor cells.D, to mass culture of cells containing recombinant DNA, detection of foreign gene is expressed.9, the construction of gene library name three methods in restructuring the son screening process and advantages.Selection of antibiotic resistance, resistance insert inactivation, blue - white spot screening or differential PCR screening, screening, DNA probeMost of the cloning vector were with antibiotic resistance genes (resistance to ampicillin,tetracycline). When the plasmid into e. coli, the resistance of bacteria to win, not to have no resistance. But it can't distinguish whether has been restructured.In contains two resistant gene carrier, if the exogenous DNA fragments inserted into one of the genes and lead to the gene inactivation, can be used two separate tablet containing different drugs controlled screening positive for restructuring. Such as pUC plasmid containing LacZ gene (beta galactose glucoside enzyme) encoding this enzyme can decompose the chromogenic substrate X - gal (5 - bromine - chlorine - 4-3 - indole - beta - D - galactose glucoside) produce blue, so that the strain to blue. After the exogenous DNA insertion, LacZ gene cannot express, with a white strains, screening of recombinant bacteria.10, through explaining the basic process of embryonic stem cells to obtain transgenic animals? Embryonic stem cells, embryonic stem cell, ES) : is the embryonic development of embryonic cells, can be artificial cultivation proliferate and differentiate into other types of cells function. The cultivation of the ES cells:Separation of the inner cell mass of blastocysts were sent to culture. ES in the culture without feeding layer will grow into a variety of functions such as muscle cells, N cells, in the culture contains fibroblasts ES will keep differentiation function.The ES can be genetic operation, do not affect its differentiation function can be fixed point integration, solved the problem of the random integration. To embryonic stem cells into exogenous gene, and then implanted to stay pregnant female uterus, develop into baby mice, hybrid homozygous mice.1) the genes have been cloned into a expression vector:...GA TA the GAA TTC ATG GGT CCG the GAA ACT CTG TAC the TGC TAA TA GGA TCC GGCT... (underlined BamHI and EcoRI respectively enzyme loci) will be a third codon by encoding the gene codon of Glu into Arg (CGU), and in n-terminal add the following five amino acids of codon (with e. coli preferred using codon). Met - Phe - Pro - Ala - Leu2) have A and B two genes ((complete ORF), now should be AB gene end-to-end series expression, and at the end of A gene with EcoRI and B before plus enzyme BamHI site. Please specify experimental design train of thought, and highlights the key attention experimental steps.3) chitinase known widely exists in various kinds of organisms, insects and other arthropods in chitinase involved in skin replacement and transfer and nutrition digestion. Chitin content at a particular stage of insect growth must be maintained at a precise level; Transient expression of chitinase showed that the enzyme expressed in inappropriate time or appropriate period do not express, have adverse influence on the growth of insects.Have insect chitinase gene cloning:The moth tobacco Manduca sextaSilkworm BombyxmoriParasitic wasps ChelonusThe United States Hyphantriacunea mothsBut there is no chitinase gene of cotton bollworm.Use has studied gene manipulation method and rt-pcr technique design experiments, chitinase gene cloning of cotton bollworm, write the detail process.。

最新高二英语冠词题20套(带答案)一、单项选择冠词1．______ advertisement uses ______ words and pictures the advertisers think of to convince people to buy ______ product or________ service, or to believe in an idea.A．An; the; a; / B．An; /; the; aC．The; /; a; / D．The; the; /; the【答案】A【解析】考查冠词。

句中an advertisement泛指广告；the words and pictures特指广告中所用的文字和图片；a product一件产品；service服务，为不可数名词，故选A。

2．Surprisingly, years back, when playing American Captain in Marvel’s movie universe became ______ possibility, Chris Evans nearly turned ______ role down.A．the; a B．the; theC．a; the D．/; the【答案】C【解析】C句意：令人惊讶的是,几年前,当在电影宇宙中扮演美国队长成为可能的时候,克里斯·埃文斯几乎拒绝了这个角色。

第一空填a，表示“一种可能性”，第二空填the，特指“American Captain这个角色”，所以选C。

3．I’ve learned that your company has ____ vacant position for a secretary. I’m calling to apply for ____ job.A．a; the B．a; a C．/; a D．/; the【答案】A【解析】试题分析:一个空缺职位，泛指，所以用a; 申请这一职位，指前面提到的空缺职位，特指用the。