Preface 兰氏化学手册

Intended Use:For In Vitro Diagnostic UseHeat induced antigen retrieval of formalin-fixed paraffin-embedded (FFPE) tissues for immunohistochemistry (IHC) procedures. The clinical interpretation of any staining or its absence should be complimented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.Summary & Explanation:Diva Decloaker is a heat retrieval solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers including citrate buffer, EDTA or high pH tris buffers. Antibody titers are doubled and tripled when compared to citrate buffer, pH 6.0. Diva Decloaker incorporates Assure™ tech nology, a color-coded high temperatures pH indicator solution. The end-user is assured by visual inspection that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures and will help prevent the possibility of losing pH sensitive antigens. Diva Decloaker is non-toxic, non-flammable, odorless and sodium azide and thimerosal free.Known Applications:Immunohistochemistry (formalin-fixed paraffin-embedded tissues) Supplied As:100mlDiva Decloaker, 10X concentrate (DV2004LX)500mlDiva Decloaker, 10X concentrate (DV2004MX)Materials and Reagents (Needed But Not Provided): Microscope slides, positively chargedDesert Chamber* (Drying oven)Positive and negative tissue controlsXylene (Could be substituted with xylene substitute*)Ethanol or reagent alcoholDecloaking Chamber* (Pressure cooker)Deionized or distilled waterWash buffer*(TBS/PBS)Enzyme digestion*Avidin-Biotin Blocking Kit*(Labeled Streptavidin Kits Only) Peroxidase block*Protein block*Primary antibody*Negative control reagents*Detection kits*Detection components*Chromogens*Hematoxylin*Bluing reagent*Mounting medium** Biocare Medical Products: Refer to a Biocare Medical catalog for further information regarding catalog number and ordering information. Certain reagents listed above are based on specific application and detection system used. Storage and Stability:Store at room temperature. Do not use after expiration date printed on vial. If reagents are stored under conditions other than those specified in the package insert, they must be verified by the user. Diluted reagents should be used promptly; any remaining reagent should be stored at room temperature.Protocol Recommendations:1. Deparaffinize tissues and hydrate to water. If necessary, block for endogenous peroxidase and wash in DI water.2. Dilute concentrated Diva Decloaker at a ratio of 1:10 (1 ml Diva to 9 ml of deionized water).3. Place slides into 1X retrieval solution in a slide container (e.g. Coplin Jar, Tissue -Tek™ staining dish or metal slide canister).4. Retrieve sections under pressure using Biocare's Decloaking Chamber. Follow the recommendations on the antibody data sheet and Decloaking Chamber User Manual.5. Check solution for appropriate color change. (See Technical Note #1)6. Gently rinse by gradually adding DI water to the solution, then remove slides and rinse with DI water.Technical Notes:1. Concentrated Diva Decloaker is a bright yellow color. RTU or 1X solution is a pale yellow color. When the solution reaches 80-125°C, the solution turns yellow and indicates that the high temperature solution is at correct pH. Should the pH rise above 7.0, the solution turns a fuschia red color. Should the pH drop too low, thesolution turns a pink color.2. If using Biocare’s Desert Chamber Pro (a programmable turbo-action drying oven), dry sections at 25ºC overnight or at 37ºC for 30-60 minutes and then dry slides at 60ºC for 30 minutes.3. Use positive char ged slides (use Biocare’s Kling-On HIER Slides) and cut tissues at 4-5 microns. Do not use any adhesives in the water bath. Poor fixation and processing of tissues will cause tissue sections to fall off the slides, especially fatty tissues such as breast. Tissues should be fixed a minimum of 6-12 hours.4. Protocol time and temperatures for HIER can vary depending on the Decloaking Chamber model used. Please refer to the relevant Decloaking Chamber manual for appropriate protocol times and temperatures.Limitations:The protocols for a specific application can vary. These include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue section thickness and detection kit used. Due to the superior sensitivity of these unique reagents, the recommended incubation times and titers listed are not applicable to other detection systems, asresults may vary. The data sheet recommendations and protocols are based on exclusive use of Biocare products. Ultimately, it is the responsibility of the investigator to determine optimal conditions. The clinical interpretation of any positive or negative staining should be evaluated within the context of clinical presentation, morphology and other histopathological criteria by a qualified pathologist. The clinical interpretation of any positive or negative staining should be complemented by morphological studies using proper positive and negative internal and external controls as well as other diagnostic tests.Catalog Number: DV2004 LX, MX Description: 100, 500 ml, concentrateQuality Control:Refer to CLSI Quality Standards for Design and Implementation of Immunohistochemistry Assays; Approved Guideline-Second edition (I/LA28-A2). CLSI Wayne, PA, USA (). 2011 Precautions:1. This product is not classified as hazardous. The preservative used in this reagent is Proclin 300 and the concentration is less than 0.25%. Overexposure to Proclin 300 can cause skin and eye irritation and irritation to mucous membranes and upper respiratory tract. The concentration of Proclin 300 in this product does not meet the OSHA criteria for a hazardous substance. Wear disposable gloves when handling reagents.2. Specimens, before and after fixation, and all materials exposed to them should be handled as if capable of transmitting infection and disposed of with proper precautions. Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water.3. Microbial contamination of reagents may result in an increase in nonspecific staining.4. Incubation times or temperatures other than those specified may give erroneous results. The user must validate any such change.5. Do not use reagent after the expiration date printed on the vial.6. The SDS is available upon request and is located at /.7. Consult OSHA, federal, state or local regulations for disposal of any toxic substances. Proclin is a trademark of Rohm and Haas Company, or of its subsidiaries or affiliates.Troubleshooting:Follow the antibody specific protocol recommendations according to data sheet provided. If atypical results occur, contact Biocare's Technical Support at 1-800-542-2002.。

1. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :Presatovir Catalog No. :HY-16727CAS No. :1353625-73-61.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USA Tel:609-228-6898Fax:609-228-5909E-mail:1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:GS-5806Formula:C 24H 30ClN 7O 3S Molecular Weight:532.06CAS No. :1353625-73-64. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician. Skin contactSafety Data SheetRevision Date:Jun.-3-2021Print Date:Feb.-17-2023Inhibitors •Screening Libraries•ProteinsRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance SolidOdor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 2Additional informationThis information is based on our current knowledge. However the chemical, physical, and toxicological properties have not been completely investigated.12. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)Proper shipping name: Not dangerous goodsUN number: -Class: -Packing group: -IMDGProper shipping name: Not dangerous goodsUN number: -Class: -Packing group: -IATAProper shipping name: Not dangerous goodsUN number: -Class: -Packing group: -15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2023 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel:609-228-6898Fax:609-228-5909E-mail:***********************Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

化学品安全技术说明书本安全技术说明书依据如下要求编写：GB/T 16483-2008, GB/T 17519-2013产品名称 Buffer pH4.00 (Red) ± 0.01 @ 20°C 修订日期 29-7月-2021 SDS编号 REA1040C版本 1第1部分第1部分：：化学品及企业标识化学品标识 产品名称 Buffer pH4.00 (Red) ± 0.01 @ 20°C其他辨识方法 SDS编号 REA1040C 纯物质纯物质／／混合物 混合物供应商信息电子邮件地址产品安全部门电子邮件:************************应急咨询电话应急咨询电话危险品或危险品事故 溅出,泄漏,火灾或事故请致电化学品运输紧急应变中心美国和加拿大联系电话: 1-800-424-9300 其他国家和地区联系电话:+1 703-741-5970 应急咨询电话无资料化学品的推荐用途和限制用途供应商艾吉析科技(上海)有限公司上海市静安区嘉地中心北京西路968号 901-903室 中国总机:400-921-6156 传真*************_____________________________________________________________________________________________化学品安全技术说明书本安全技术说明书依据如下要求编写：GB/T 16483-2008, GB/T 17519-2013产品名称 Buffer pH4.00 (Red) ± 0.01 @ 20°C SDS编号 REA1040C修订日期 29-7月-2021推荐用途 实验室用途 限制用途无资料第2部分第2部分：：危险性概述GHS危险性类别皮肤腐蚀/刺激 类别2 严重眼损伤/眼刺激 类别2A标签要素信号词警告危险性说明 造成皮肤刺激造成严重眼刺激防范说明 预防措施作业后彻底清洗脸部、手部和任何暴露的皮肤戴防护手套/穿防护服/戴防护眼罩/戴防护面具事故响应如皮肤沾染：用大量水和肥皂清洗如发生皮肤刺激：求医/就诊脱掉受沾染的衣服，清洗后方可重新使用如进入眼睛：用水小心冲洗几分钟。

普鲁士蓝染色试剂盒(PERLS STAIN，伊红法)
产品简介：
Perls 普鲁士蓝是非常经典的组织化学反响，是显示组织内三价铁的一种敏感、传统优良的方法，其染色原理为：亚铁氰化钾溶液使三价铁离子从蛋白质中被稀盐酸别离出来，三价铁与亚铁氰化钾反响，生成一种不溶解的蓝色化合物即三价铁的亚铁氰化物普鲁士蓝，所以该反响被称为普鲁士蓝反响。

三价铁的亚铁氰化物是一种很稳定的化合物，在反响后可用红色染色剂进展复染，如核固红、伊红、中性红等。

含铁血黄素〔Hemosiderin〕是一种血红蛋白源性色素，为金黄色或棕黄色颗粒，因其含铁、金黄色，故称为含铁血黄素。

当红细胞被巨噬细胞吞噬后，在溶酶体酶的作用下，血红蛋白被分解为不含铁的橙色血质和含铁的含铁血黄素。

Perls 普鲁士蓝反响〔Prussian blue reaction〕又称为含铁血黄素染色，即经过亚铁氰化钾和稀酸处理后可以产生蓝色，常见于吞噬细胞内会间质内，主要显示三价铁盐。

Perls stain 常用于显示局部组织内各种出血性病变，常见于吞噬细胞内。

在判断含铁血黄素沉积时，用Perls 反响可以得到证实，该染色方法可以很好的区分含铁血黄素和其他色素。

该染色液稳定性好、可以长期保存、不易产生沉淀、应用范围广、可以进展复染。

普鲁士蓝染色试剂盒〔Perls stain，伊红法〕，其复染液采用伊红染色液，也是常用的复染液，该复染液染色时间较核固红染色液要短。

保存：RT避光保存，有效期 1 年。

关键词：普鲁士蓝染色试剂盒(PERLS STAIN，伊红法)
普鲁士蓝染色试剂盒(PERLS STAIN，伊红法)相关染色产品:。

兰氏化学手册-15
摘要：
1.兰氏化学手册-15 简介
2.兰氏化学手册-15 的主要内容
3.兰氏化学手册-15 的特点和价值
4.兰氏化学手册-15 的应用领域
正文：
《兰氏化学手册》是一本享有国际盛誉的化学工具书，其第十五版（兰氏化学手册-15）更是集大成者，为广大化学工作者提供了丰富的化学知识和实践指导。

兰氏化学手册-15 的主要内容包括：化学基本理论、化学实验技术、化学品性质与用途、化学工艺流程、化学分析方法等。

此外，该手册还涵盖了生物化学、物理化学、有机化学、无机化学等多个化学分支领域的知识，为化学工作者提供了全面的参考资料。

兰氏化学手册-15 的特点在于其严谨的科学态度、系统性的知识架构和实用性的指导方法。

该手册在保持理论严谨性的同时，注重实践应用，使得化学知识更加贴近实际。

此外，手册的内容不断更新，以适应科技发展的需求，使其具有很高的时效性和参考价值。

兰氏化学手册-15 的应用领域非常广泛，既适用于化学科研工作者、工程师，也适用于化学教师和学生。

在化学实验室、化工厂、教育机构等场所，都能看到这本手册的身影。

同时，该手册也是图书馆、资料室的必备藏书，为化
学爱好者提供了丰富的学习资源。

之邯郸勺丸创作蛋白质的定位与蛋白质裂解液的选择：全细胞：NP-40或RIPA细胞质（可溶）：Tris-HCl细胞质（细胞骨架）：Tris-Triton细胞膜：NP-40或RIPA细胞核：RIPA线粒体：RIPARIPA裂解液配方1ml RIPA buffer contains 35μl proteinase inhibitorand 10μl phosphatase inhibitor加水到 100mlAprotinin 10μg/μl ；用10mM HEPES-KOH（PH8.0）溶解,备用.●Nonidet P-40简称NP-40,乙基苯基聚乙二醇,经常使用非离子性去垢剂.●Na-deoxycholale,脱氧胆酸钠,离子型去垢剂.离子型去垢剂主要作用有：裂解细胞；溶解蛋白,尤其是可以溶解一些难溶于水的蛋白,如膜蛋白等；很适合做WB,但在Co-IP中使用,需要谨慎.●亮抑霉肽（Leupeptin）：抑制丝氨酸蛋白酶包含胰蛋白酶、纤溶酶、猪胰激肽原酶）和半胱氨酸蛋白酶（包含木瓜蛋白酶和组织蛋白酶B）●抑肽素A（Pepstatin A）抑制各类天门冬氨酸蛋白酶,例如组织蛋白酶D、肾素、胃蛋白酶、细菌天门冬氨酸蛋白酶和HIV蛋白酶；●胰凝乳蛋白酶抑制剂（Chymostatin）抑制具有糜蛋白酶类特异性丝氨酸蛋白酶（包含糜蛋白酶、胃促胰酶、组织蛋白酶 G）和大多数丝氨酸蛋白酶（包含组织蛋白酶B,H,L）●抗木瓜蛋白酶（Antipain）抑制木瓜蛋白酶、胰蛋白酶和纤溶酶.●氟化钠抑制酸性磷酸酶●正钒酸钠：抑制碱性磷酸酶和酪氨酸磷酸酶（tyrosine phosphatase）●焦磷酸钠（sodium pyrophosphate）：抑制丝氨酸-苏氨酸磷酸酶（serine－threonine phosphatase） .在与蛋白相关的检测中,首先最关头的一步即是蛋白质的提取.蛋白质的提取过程中,我们要经常加和蛋白酶抑制剂以避免蛋白质的降解.另外在磷酸化蛋白的研究过程中,磷酸酶抑制剂也是必不成少的,本文总结了经常使用的蛋白酶抑制剂PMSF,Leupeptin 亮肽素,Aprotinin抑肽酶,Pepstatin胃蛋白酶抑制剂,EDTA-Na2等以及磷酸酶抑制剂NaF氟化钠,Na3VO4 原矾酸钠 ,BETA-glycerophosphate 甘油磷酸钠,Na2P2O4 焦磷酸钠等.对这些蛋白酶抑制剂的溶解配制,贮存液与任务液浓度,保管都做了详细的说明.蛋白酶抑制剂PMSF：特性：丝氨酸蛋白酶抑制剂,如胰凝乳蛋白酶,胰蛋白酶和凝血酶,也抑制半胱氨酸蛋白酶如木瓜蛋白酶（可逆的地面处理）.溶解性：溶于异丙醇,乙醇,甲醇和丙二醇果>10mg/ml.在水溶液中不稳定.在100%异丙醇, +25℃时稳定至少9个月份子量：174.2使用：贮存浓度：200mM,任务浓度：1mMLeupeptin 亮肽素特性：抑制丝氨酸和半胱氨酸蛋白酶如胰蛋白酶,木瓜蛋白酶,纤溶酶,和组织蛋白酶B溶解性：高度溶于水（1mg/ml）.4℃一周稳定,分红小份冷冻在-20℃至少6个月份子量：C20H38N6O4 x 1/2 H2SO4:475.6C20H38N6O4 x 1/2 H2SO4 x H2O:493.6使用：贮存浓度：1mg/ml,任务浓度0.5 ug/ml (1mM)Aprotinin抑肽酶特性：丝氨酸蛋白酶抑制剂,抑制纤维蛋白溶酶, 激肽释放酶,胰蛋白酶,糜蛋白酶的高活性.不抑制凝血酶或因子X.溶解性：易溶于水（10mg/ml）或缓冲液（例如,tris,0.1M,pH8.0）.pH 约7-8的溶液在4℃可保管1周,分装保管在-20℃可至少保管6个月.避免频频冻融, pH>12.8的碱性环境可使其灭活.份子量：6,512使用：贮存浓度:1mg/ml, 任务浓度：0.06–2.0 ug/ml(0.01–0.3 uM)Pepstatin胃蛋白酶抑制剂特性：抑制天冬氨酸（酸）蛋白酶如胃蛋白酶,肾素,组织蛋白酶D,凝乳酶, 许多微生物酸性蛋白酶溶解性：溶于甲醇约1mg/ml；可溶于乙醇,过夜溶解可达到1 mg/ml；在6当量乙酸中溶解度为300ug/ml.4℃稳定一周,分装储存于-20℃时可保管1个月份子量： 685.9使用：贮存浓度：1mg/ml,使用浓度：0.7 μg/ml(1μM)EDTA-Na2特性：金属蛋白酶抑制剂溶解性：溶于水至0.5M,在pH8-9的条件下,4℃稳定至少6个月份子量: 372.24使用：任务浓度：0.2–0.5 mg/ml(0.5–1.3 mM),不需现用现配,在溶液pH值调至8-9时再加入.磷酸酶抑制剂NaF氟化钠溶解性：溶于水份子量： 41.99使用：贮存液：5M 任务浓度：10-20mMNa3VO4 原矾酸钠份子量：183.91溶解性：溶于水,我们采办过来的是原矾酸钠.原矾酸钠需要经过处理以后才干成为激活的矾酸钠,激活的矾酸钠才具有抑制去磷酸化的作用.原矾酸钠酿成激活的矾酸钠的过程是:100mM原矾酸钠激活储存液配制(1).取0.183克的原矾酸钠溶解于10ml的双蒸水中,加酸调节PH至10(颜色变黄)(2).煮至无色(3).室温冷却(4).重调PH至10(5)重复（１）（２）（３）（４）直至溶液坚持无色,并且ＰＨ稳定于10,分装100ul/管,每10ml裂解液加一管.(终浓度为1mM),-20度保管.使用：贮存液：100mM 任务浓度：1mMBETA-glycerophosphate 甘油磷酸钠溶解性：溶于水份子量：306.11使用：贮存液：100mM 任务浓度：25mMNa2P2O4 焦磷酸钠溶解性：溶于水份子量：265.9贮存液：100mM, 任务浓度：1-2 mMSDS ,NP-40 ,TritonX-100这三种去垢剂的作用是不合的,或者说作用力量强弱不合.SDS属于离子型去垢剂,最厉害,基本可以把细胞完全破掉,DNA会释放出来,裂解液变得很粘稠.NP-40是很温和的去垢剂,1%浓度的基本可以破坏掉胞膜,而对核膜破坏的作用弱,结合特定的buffer可以获得胞浆蛋白.TritonX-100的能力介于NP40和SDS之间,偏向于NP40,也是经常使用的细胞裂解液成分之一,在呵护蛋白活性方面有一定作用（SDS 基本会使蛋白变性失活）.Brij Buffer I。

兰氏化学手册
兰氏化学手册是一本涵盖了广泛化学知识的参考书籍。

在书中，你可以找到无数化学元素的信息，例如原子量、电子结构、化学反应等等。

除此之外，兰氏化学手册还包含了许多化学实验的详细操作步骤和注意事项。

这些实验涵盖了化学反应、化学分析、化学合成等多个方面，非常适合化学学习者和研究人员用作参考。

此外，兰氏化学手册还提供了关于化学工程、环境化学等方面开展研究和生产所需的许多数据和信息。

其中包括化学反应速率、热力学数据、物理化学性质等内容，这些数据对于理解和解决实际问题非常有帮助。

总之，兰氏化学手册是一本非常全面、详细的化学参考书籍，它为化学学习者和研究人员提供了大量的信息和参考资料。

The data on this sheet represent typical values. Since application variables are a major factor in product performance, this information should serve only as a general guide. Axaltaassumes no obligation or liability for use of this information . UNLESS AXALTA AGREES OTHERWISE IN WRITING, AXALTA MAKES NO WARRANTIES, EXPRESS ORIMPLIED, AND DISCLAIMS ALL IMPLIED WARRANTIES INCLUDING WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR FREEDOM FROM PATENT INFRINGEMENT. AXALTA WILL NOT BE LIABLE FOR ANY SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES. Your only remedy for any defect in thisproduct is the replacement of the defective product, or a refund of its purchase price, at our option. The information in this sheet, as well as the products referenced herein, shall beGENERAL INFORMATIONAxalta ’s standard solids PRE-CAT SEALER is a highly versatile pre-catalyzed lacquer sanding sealer formulated for outstandingdurability and ease of use. It is recommended for use under Valspar’s ENVIRO SUPERLAC ™ Pre-cat lacquers. It is quick drying with excellent flow and leveling properties. It also has excellent mar, water, solvent & household chemical resistance.1. PRODUCTS• 371-1946-002. MIXING RATIO• All products should be stirred well before use and, forbest results, continuously agitated while in use.3. SHELF LIFE @ 77°F (25°C)• 6 months from manufacturing date4. CLEAN UP• Dispose of dirty solvent and cleaning rags in a safe andcompliant manner. Solvent or lacquer soaked rags should be stored in water-filled, closed containers prior to disposal.5. ADDITIVES• In the event of blushing or blistering, add Axalta 390-9303-00 Lacquer Retarder to extend dry time. See Additional Notes portion of this document for details.6. SURFACE PREPARATION• Surface must be clean and dust free with a moisturecontent of 6-8% prior to finishing. Remove all dust, dirt, wax and wood marks. Proper sanding and preparation of the wood is critical to achieving consistent results.• On new wood, finish sand surface with 150-180 grit sandpaper• On previously finished wood, remove all old paint or varnish and follow new wood procedure.7. COMPANION PRODUCTS• 371-1980/90 Series ENVIRO SUPERLAC ™• 371-1990 Series SUPERLAC ™ ELITE Pre-CatalyzedLacquers• NUC3290 Series SUPERLAC ™ Plus Pre-Catalyzed Lacquers8. TECH NOTES• If reduction is necessary, use Axalta 390-7001-00 LacquerThinner. See Additional Notes portion of this document for additional details.9. SUBSTRATES• Commonly used furniture and cabinetry woods • MDF/HDFNOTE: Not to be used on exterior applications.10. APPLICATION• See application notes foe detailed instructions.11. FLASH / DRY TIMESAIR DRY @ 77°F (25°C)AIR PRESSURES14. PHYSICAL DATAproduct is the replacement of the defective product, or a refund of its purchase price, at our option. The information in this sheet, as well as the products referenced herein, shall be。

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请查看随附由我们的团队以及患者咨询委员会编制的化疗课程手册，这是我们向您提供的一项资源。

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