NanoDrop 的英文校正手册

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Calibration CheckIt is recommended that a pedestal calibration check be performed every six months to verify that the instrument is performing within specifications.A vial of Thermo Scientific CF-1 calibration fluid is required to run the calibration check procedure. CF-1 is an aqueous potassium dichromate (K2Cr2O7) solution used to confirm the pathlength accuracy of NanoDrop2000/2000c spectrophotometers. It is roughly ten times more concentrated than other commercially availableK2Cr2O7 solutions used to confirm the calibration of standard spectrophotometers and is available through Thermo Fisher Scientific or your local distributor. Refer to the included material data safety sheet provided with the vial and take the appropriate laboratory precautions.Pedestal Calibration Check Procedure1. Ensure the measurement pedestals are clean and that a 1 µL water sample “beads” up on the lowerpedestal.2. Open the NanoDrop 2000/2000c software, and using the Diagnostics task bar, and choose CalibrationCheck.3. Enter the actual Target Absorbance stated on the CF-1 vial in the appropriate field as shown in the imagebelow.4. Add 1 µL of dH2O to the pedestal, lower the arm and click Blank.5. Wipe the upper and lower pedestals using a dry laboratory wipe.6. Vigorously shake the CF-1 vial to thoroughly mix the solution. Ensure that all of the solution has collected inthe bottom portion of the ampoule before carefully breaking open the vial.Note: The CF-1 calibration fluid is supplied in a single use vial. CF-1 must be used within one hour of opening the vial. Exposure to the environment or transferring of the fluid to another container mayresult in a significant change in concentration.7. Follow the on-screen prompts in the Customer Guidance text box. Using individual 1µL aliquots of the CF-1Calibration Fluid, measure 10 replicates. After the 10th measurement, the calibration check results will be displayed on-screen in the Customer Guidance text box.8. If the instrument does not pass the calibration check using 1 µL samples, immediately repeat the procedureagain using 2 µL samples.If the procedure fails with 1 µL volumes but passes with 2 µL volumes, it is an indication that the pathlengths are within specifications but the pedestals may not be properly conditioned. Refer to the Pedestal Reconditioning procedure for more information.If the instrument still does not pass the calibration check using 2 µL samples recalibration is required. Contact Technical Support. Outside of the US and Canada, please contact your local NanoDrop products distributor.A .JPG of the final results is automatically archived on the hard drive at the following location:Vista: C:\Users\Public\Public Documents\Thermo\Autosave\CalibCheckXP: C:\Documents and Settings\AllUsers\Documents\Thermo\NanoDrop2000\Autosave\CalibCheckCuvette Calibration CheckAlthough a specific cuvette calibration check procedure is not available for the NanoDrop 2000c, we suggest the use of 10%T and 50%T neutral density filters such as those available from Thermo Scientific to monitor performance. The table below compliments the expected performance table found in the SPECTRONIC™ Standards Operators Manual.NanoDrop 2000cExpected performance usingSPECTRONIC™ Standards KitWhite 50%T ±0.011AWhite 10%T ±0.044AYellow 50%T ±0.011AYellow 10%T ±0.044A0%T, full spectrum 2A*Wavelength Accuracy ±1.5nmStray Radiant Energy 220 2 A*Stray Radiant Energy 340 2 A*Stray Radiant Energy 400 2 A**These light-blocking standards should achieve at least 2 A at the wavelength of interest.HardwareConnection ErrorsIf your instrument operates properly most of the time, but connection errors (e.g codes 1010, 1029 or 1039) appear intermittently, the instrument may not be receiving power, or recognizing the USB connection. Ensure that the USB and power cables are plugged into the back of the instrument, and that the instrument is receiving power.If the error persists, contact Technical Support. Outside of the US and Canada, please contact your local NanoDrop products distributor.Power RestartMany instrument issues can be addressed by a simple power restart. For error codes 1019, 1054, 1055 and 1056, follow the directions below:。