HaloPROTAC 2 1799506-06-1 GlpBio
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Peptides, Inhibitors, Agonists
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Product Data Sheet
Product Name: HaloPROTAC 2
Cat. No.: GC32831
Chemical Name:
CHEMICAL PROPERTIES Cas No.: 1799506-06-1
Molecular Formula: C38H59ClN4O9S
Molecular Weight: 783.41
Storage: Powder
Solubility: Soluble in DMSO
Chemical Structure:
Background
HaloPROTAC 2, a chloroalkane−containing PROTAC, induces degradation of HaloTag fusion proteins.
HaloPROTAC 1 and HaloPROTAC2, containing different linker lengths, are synthesized. Their ability to degrade GFP−HaloTag7,
(stably expressed in HEK 293 cells) is tested tby flow cytometry, measuring changes in mean fluorescence intensity. While 24 hour
treatment with HaloPROTAC 1 leads to less than 20% degradation, the longer HaloPROTAC2 leads to nearly 70% degradation of
GFP−Halotag7 at 2.5 μM[1].
References:
[1]. Buckley DL, et al. HaloPROTACS: Use of Small Molecule PROTACs to Induce Degradation of HaloTag Fusion Proteins. ACS
Chem Biol. 2015 Aug 21;10(8):1831-7.
Research Update
1. Effect of Hydrofluoric Acid Concentration and Etching Time on Bond Strength to Lithium Disilicate Glass Ceramic. Oper Dent. 2017 Nov/Dec;42(6):606-615. doi: 10.2341/16-215-L. Epub 2017 Jul 14. PMID:28708007
Abstract
The aim of this study was to evaluate the influence of different concentrations of hydrofluoric acid (HF) associated with varied etching
times on the microshear bond strength (μSBS) of a resin cement to a lithium disilicate glass ceramic. Two hundred seventy
-five ceramic blocks (IPS e.max Press [EMX], Ivoclar Vivadent), measuring 8 mm × 3 mm thickness, were randomly distributed into five
groups according to the HF concentrations (n=50): 1%, 2.5%, 5%, 7.5%, and 10%.
2. Does acid etching morphologically and chemically affect lithium disilicate glass ceramic surfaces? J Appl Biomater Funct Mater.
2017 Jan 26;15(1):e93-e100. doi: 10.5301/jabfm.5000303. PMID:27647389
Abstract
BACKGROUND: This study evaluated the surface morphology, chemical composition and adhesiveness of lithium disilicate glass
ceramic after acid etching with hydrofluoric acid or phosphoric acid.METHODS: Lithium disilicate glass ceramic specimens polished by
600-grit silicon carbide paper were subjected to one or a combination of these surface treatments: airborne particle abrasion with 50-
μm alumina (AA), etching with 5% hydrofluoric acid (HF) or 36% phosphoric acid (Phos), and application of silane coupling agent (Si).
3. Fatigue failure load of feldspathic ceramic crowns after hydrofluoric acid etching at different concentrations. J Prosthet Dent. 2018
Feb;119(2):278-285. doi: 10.1016/j.prosdent.2017.03.021. Epub 2017 May 26. PMID:28552291
Abstract
STATEMENT OF PROBLEM: Hydrofluoric acid etching modifies the cementation surface of ceramic restorations, which is the same
surface where failure is initiated. Information regarding the influence of hydrofluoric acid etching on the cyclic loads to failure of ceramic
crowns is lacking.PURPOSE: The purpose of this in vitro study was to evaluate the influence of different hydrofluoric acid concentrations on the fatigue failure loads of feldspathic ceramic crowns.
