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Toxicological effect of MPA–CdSe QDs exposure on zebrafish embryo and larvae

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ToxicologicaleffectofMPA–CdSeQDsexposureonzebrafishembryoandlarvae

WeiZhanga,b,c,⇑,KuangfeiLina,b,c,⇑,XueSuna,b,c,QiaoxiangDongd,ChangjiangHuangd,HuiliWangd,

MeijinGuoe,XinhongCuif

aStateEnvironmentalProtectionKeyLaboratoryofEnvironmentalRiskAssessmentandControlonChemicalProcess,EastChinaUniversityofScienceandTechnology,Shanghai200237,PRChinabShanghaiKeyLaboratoryofFunctionalMaterialsChemistry,EastChinaUniversityofScienceandTechnology,Shanghai200237,PRChinacSchoolofResourceandEnvironmentalEngineering,EastChinaUniversityofScienceandTechnology,Shanghai200237,PRChinadZhejiangProvincialKeyLabforTechnologyandApplicationofModelOrganisms,WenzhouMedicalCollege,Wenzhou325035,PRChinaeStateKeyLaboratoryofBioreactorEngineering,EastChinaUniversityofScienceandTechnology,Shanghai200237,PRChinafShanghaiInstituteofLandscapeGardening,Shanghai200233,PRChina

articleinfo

Articlehistory:Received3October2011Receivedinrevisedform11March2012Accepted4April2012Availableonline15May2012

Keywords:MPA–CdSeQDsZebrafishembryoZebrafishlarvaeDevelopmentaltoxicityBehavioraltoxicityabstract

Cadmiumselenium(CdSe)quantumdots(QDs)aresemiconductornanocrystalsthatholdwiderangeofapplicationsandsubstantialproductionvolumes.Duetouniquecompositionandnanoscaleproperties,theirpotentialtoxicitytoaquaticorganismshasincreasinglygainedagreatamountofinterest.However,theimpactofCdSeQDsexposureonzebrafishembryoandlarvaeremainsalmostunknown.Therefore,thelabstudywasperformedtodeterminethedevelopmentalandbehavioraltoxicitiestozebrafishundercontinuousexposuretolowlevelCdSeQDs(0.05–31.25mgLÀ1)coatedwithmercaptopropionicacid(MPA).TheresultsshowedMPA–CdSeexposurefromembryotolarvaestageaffectedoverallfitness.Ourfindingsforthefirsttimerevealedthat:(1)The120hLC50ofMPA–CdSeforzebrafishwas1.98mgLÀ1;(2)embryosexposedtoMPA–CdSeresultedinmalformationsincidenceandlowerhatchrate;(3)abnormalvascularofFLI-1transgeniczebrafishlarvaeappearedafterexposuretoMPA–CdSeincludingvascularjunction,bifurcation,crossingandparticleappearance;(4)larvaebehaviorassessmentshowedduringMPA–CdSeexposurearapidtransitionfromlight-to-darkelicitedasimilar,briefburstandahigherbasalswimmingrate;(5)MPA–CdSeinducedembryoscellapoptosisintheheadandtailregion.ResultsoftheobservationsprovideabasicunderstandingofMPA–CdSetoxicitytoaquaticorganismsandsuggesttheneedforadditionalresearchtoidentifythetoxicologicalmechanism.Ó2012ElsevierLtd.Allrightsreserved.

1.Introduction

Quantumdots(QDs)holdconsiderablepromiseduringthelast

decadesinsolarcell,electronics,lifesciences,biomedicalimaging,

diagnostics,andphotovoltaicsapplicationsbecauseoftheirphysi-

calsize,brightfluorescence,narrowemissionspectra,broad

absorptioncoefficientsspectra,andhighphotostability(Chanand

Nie,1998;Jretal.,1998;Wangetal.,2004;Michaletetal.,

2005;BhattandTripathi,2011).QDscoresconsistofavarietyof

metalcomplexes,whichmayexertdetrimentalconsequencesnot

onlybytheirnanoscalepropertiesbutalsobychemicalinterac-

tionswithcellsatrelativelylowconcentrations.Torenderthem

biologicallycompatible/active,newlysynthesizedQDsaregiven

secondarycoatings,whichimproveswatersolubility,coredurabil-

ity,andsuspensioncharacteristics(Lovricetal.,2005;Medintz

etal.,2005;Wangetal.,2008).However,QDswithvariousorganiccoatingswouldbesubjecttomodificationordegradationunder

environmentalconditions,andhencewouldbecometoxic(Kim

etal.,2010).

Cadmiumselenium(CdSe)QDsaresemiconductornanocrystals

thatholdwiderangeofapplicationsandsubstantialproduction

volumes.Initialreportssuggesteddihydrolipoicacid-cappedCdSe

QDslackedtoxicitytowardmammalian(HeLa)andslimemold

(AX2)cells(Jaiswaletal.,2003).However,subsequentstudieson

surface-coatedCdSeQDsdemonstratedsometoxicityinvarious

celllines(Derfusetal.,2004;Kimetal.,2010).CdSeQDsare

increasinglyfoundinenvironmentalwatersamplesviawaste

streamsfromindustriesthatsynthesizeoruseCdSeandthrough

clinicalandresearchuses(Aitkenetal.,2006;Hardman,2006).

However,theimpactofCdSeQDsexposureonwaterenvironment

remainslargelyunknown.Therefore,thereisanurgentneedto

determinetheCdSeQDsinducedecotoxicologicaleffectstoaquatic

organismsandtoidentifyconvenientbiomarkersforearly

signaling.

Thepotentialtoxicityofengineerednanoparticles(NPs)to

aquaticorganismsispoorlyunderstood(Buffetetal.,2011).In

0045-6535/$-seefrontmatterÓ2012ElsevierLtd.Allrightsreserved.

http://dx.doi.org/10.1016/j.chemosphere.2012.04.012⇑Correspondingauthors.Address:Box563,130MeilongRoad,Shanghai200237,PRChina.Tel.:+862164253244;fax:+862164253988.E-mailaddresses:wzhang@ecust.edu.cn(W.Zhang),kflin@ecust.edu.cn(K.

Lin).Chemosphere89(2012)52–59

ContentslistsavailableatSciVerseScienceDirect

Chemosphere

journalhomepage:www.elsevier.com/locate/chemos

p

h

ere

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HSP90 抑制剂根赤壳菌素对斑马鱼胚胎发育的影响

HSP90 抑制剂根赤壳菌素对斑马鱼胚胎发育的影响

中国水产科学 2017年9月, 24(5): 988994 Journal of Fishery Sciences of China 研究论文

收稿日期: 2017-01-12; 修订日期: 2017-04-12. 基金项目: 国家自然科学基金项目(31372516); 上海市人才发展资金项目(201457); 上海市教育委员会“东方学者”计划支持项目. 作者简介: 罗军涛(1989−), 男, 硕士, 专业方向为发育生物学. E-mail: 1012688697@ 通信作者: 韩兵社, 副研究员. E-mail: bs-han@ DOI: 10.3724/SP.J.1118.2017.17151

HSP90抑制剂根赤壳菌素对斑马鱼胚胎发育的影响

罗军涛, 李艳, 陶筱帆, 刘玮, 倪国军, 张俊芳, 韩兵社

省部共建水产种质资源发掘与利用教育部重点实验室, 上海海洋大学 水产与生命学院, 上海 201306

摘要: 为研究热休克蛋白90(heat shock protein 90, HSP90)在斑马鱼(Danio rerio)胚胎发育中的作用, 本实验采用 2 μmol/L、5 μmol/L、10 μmol/L的HSP90抑制剂根赤壳菌素(radicicol)对斑马鱼发育期胚胎进行处理, 监测斑马鱼胚胎不同发育时期两个HSP90功能抑制标志基因BAG3 (BCL2-associated athanogene3)和HSPB1(heat shock protein beta-1)的mRNA的表达水平, 并观察不同发育时期的胚胎发育状况。结果如下: (1)实时荧光定量PCR结果显示, BAG3和HSPB1 mRNA水平在根赤壳菌素处理胚胎12 hpf (hours post-fertilization, hpf) 或24 hpf后显著增高, Western blot检测到5 μmol/L根赤壳菌素处理胚胎24 hpf后HSP70表达上调, 证明该实验条件下HSP90功能受到抑制; (2)根赤壳菌素处理后斑马鱼胚胎发育变缓, 胚胎成活率统计显示: 2 μmol/L、5 μmol/L、10 μmol/L根赤壳菌素处理24 hpf胚胎成活率分别是95%、77%、35%, 成活率随根赤壳菌素浓度的增高而降低; (3)5 μmol/L根赤壳菌素处理72 hpf可见部分个体色素沉积减少、心包膜增大、肌肉萎缩等发育畸形。研究结果证明HSP90在斑马鱼胚胎发育过程中发挥重要的作用, 根赤壳菌素在5 μmol/L时已达到较佳抑制效果且成活率较高, 而且胚胎发育出现了各种形态学变化, 为后期研究HSP90调节斑马鱼胚胎发育奠定了基础。

微塑料和纳米塑料对胃肠道及肝脏的毒性效应机制研究进展

微塑料和纳米塑料对胃肠道及肝脏的毒性效应机制研究进展

生态毒理学报

AsianJournalofEcotoxicology第18卷第4期2023年8月Vol.18,No.4Aug.2023

基金项目:国家自然科学基金青年项目(82202069) 第一作者:孙凡晰(1997—),女,硕士研究生,研究方向为环境材料与技术,E-mail:********************* *通信作者(Correspondingauthor),E-mail:*********************.cn 﫹共同通信作者(Co-correspondingauthor),E-mail:****************DOI:10.7524/AJE.1673-5897.20221025001孙凡晰,齐鑫,王靖,等.微塑料和纳米塑料对胃肠道及肝脏的毒性效应机制研究进展[J].生态毒理学报,2023,18(4):131-147

SunFX,QiX,WangJ,etal.Mechanismoftoxiceffectsofmicroplasticsandnano-plasticsongastrointestinaltractandliver:Areview[J].AsianJournal

ofEcotoxicology,2023,18(4):131-147(inChinese)

微塑料和纳米塑料对胃肠道及肝脏的毒性效应机制研

究进展

孙凡晰,齐鑫,王靖*,纪丽莲#

苏州科技大学化学与生命科学学院,苏州215000

收稿日期:2022-10-25 录用日期:2023-01-20

摘要:由于塑料制品的大量生产和使用,其废弃物降解产生的微塑料(microplastics,MPs)作为一种新型的环境污染物近年来逐

渐引起全世界的关注。持续的老化会使微塑料降解为纳米塑料(nano-plastics,NPs),在进入人体后增加对细胞的危害,因此微

塑料和纳米塑料对人体产生的毒性效应及健康危害也日益成为环境领域的研究热点。本文基于已有研究,重点阐述了人体

毒理学基础英文词汇

毒理学基础英文词汇

1 毒理学基础英文词汇 第一章 绪论 Toxicology（毒理学） environmental toxicology（环境毒理学） clinical toxicology（临床毒理学） forensic toxicology（法医毒理学） regulatory toxicology（管理毒理学） ecotoxicology（生态毒理学） in vivo（体内试验） in vitro（体外试验） in situ（原位） toxicogenomics（毒物基因组学） 第二章 毒理学基本概念 xenobiotic（外源化学物） toxicant或poison（毒物） toxin（毒素） toxicity（毒性） toxic effect（毒作用/毒效应） adverse effect (损害作用) target organ（靶器官） spectrum of toxic effects（毒效应谱） biomarker（生物学标志） graded response（量反应） quantal response（质反应） graded dose-response relationship（剂量-量反应关系/剂量-效应关系） quantal dose-response relationship(剂量-质反应关系/剂量-反应关系） median lethal dose（半数致死剂量，LD50） absolute lethal dose（绝对致死剂量，LD100） minimal lethal dose（最小致死剂量，LD01） median tolerance limit（半数耐受限量） threshold dose（阈剂量） lowest observed adverse effect level (观察到损害作用的最低剂量，LOAEL ) no-observed adverse effect level (未观察到损害作用剂量，NOAEL )， toxic effect zone（毒作用带） acceptable daily intake（每日容许摄入量，ADI） maximum allowable concentration（最高容许浓度） reference dose（参考剂量） 第三章 外源化学物在体内的生物转运和生物转化 biotransportation（生物转运） absorption（吸收） distribution（分布）

Toxic effects of di(2-ethylhexyl)phthalate on mortality, growth,

Toxic effects of di(2-ethylhexyl)phthalate on mortality, growth,

Toxicology237(2007)

126–133

Toxiceffectsofdi(2-ethylhexyl)phthalateonmortality,growth,

reproductionandstress-relatedgeneexpressionin

thesoilnematodeCaenorhabditiselegans

Ji-YeonRoh,In-HoJung,Jai-YoungLee,JinheeChoi∗

FacultyofEnvironmentalEngineering,CollegeofUrbanScience,UniversityofSeoul,90Jeonnong-dong,Dongdaemun-gu,Seoul130-743,RepublicofKorea

Received16March2007;receivedinrevisedform19April2007;accepted2May2007Availableonline18May2007

Abstract

Inthisstudy,di(2-ethylhexyl)phthalate(DEHP)toxicitiestoCaenorhabditiseleganswereinvestigatedusingmultipletoxicendpoints,suchasmortality,growth,reproductionandstress-relatedgeneexpression,focusingontheidentificationofchemical-inducedgeneexpressionasasensitivebiomarkerforDEHPmonitoring.ThepossibleuseofC.elegansasasentinelorganisminthemonitoringofsoilecosystemhealthwasalsotestedbyconductingtheexperimentontheexposureofnematodetofieldsoil.Twenty-four-hourmedianlethalconcentration(LC50)datasuggestthatDEHPhasarelativelyhighpotentialofacutetoxicitytoC.elegans.Decreasesinbodylengthandeggnumberperwormobservedafter24hofDEHPexposuremayinducelong-termalterationinthegrowthandreproductionofthenematodepopulation.BasedontheresultfromtheC.elegansgenomearrayandindicatedintheliteratures,stressproteins,metallothionein,vitellogenin,xenobioticmetabolismenzymes,apoptosis-relatedproteins,andantioxidantenzymegeneswereselectedasstress-relatedgenesandtheirexpressioninC.elegansbyDEHPexposurewasanalyzedsemi-quantitatively.Expressionofheatshockprotein(hsp)-16.1andhsp-16.2geneswasdecreasedbyDEHPexposure.ExpressionofcytochromeP450(cyp)35a2andglutathione-S-transferease(gst)-4,phaseIandphaseIIofxenobioticmetabolismenzymes,wasincreasedbyDEHPexposureinaconcentration-dependentmanner.Anincreaseinstress-relatedgeneexpressionsoccurredconcomitantlywiththedeteriorationonthephysiologicallevel,whichsuggestsanincreaseinexpressionofthosegenesmaynotbeconsideredasahomeostaticresponsebutasatoxicitythatmighthavephysiologicalconsequences.TheexperimentwiththesoilfromthelandfillsitesuggeststhatthepotentialoftheC.elegansbiomarkeridentifiedinlaboratoryconditionsshouldbecalibratedandvalidatedforitsuseinsitu.©2007ElsevierIrelandLtd.Allrightsreserved.