酶切位点汇总

常见分子实验限制性内切酶酶切位点大全公司内部编号：（GOOD-TMMT-MMUT-UUPTY-UUYY-DTTI-AatII 识别位点Acc65I 识别位点AccI 识别位点AciI 识别位点AclI 识别位点AcuI 识别位点AfeI 识别位点AflII 识别位点AflIII 识别位点AgeI 识别位点AhdI 识别位点AleI 识别位点AluI 识别位点AlwI 识别位点AlwNI 识别位点ApaI 识别位点ApaLI 识别位点ApeKI 识别位点ApoI 识别位点AscI 识别位点AseI 识别位点AsiSI 识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI 识别位点BamHI识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI 识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI 识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI 识别位点BsaXI 识别位点BseRI 识别位点BseYI 识别位点BsgI 识别位点BsiEI 识别位点BsiHKAI 识别位点BsiWI 识别位点BslI识别位点BsmAI识别位点BsmBI 识别位点BsmFI 识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI 识别位点BtgZI 识别位点BtsCI 识别位点BtsI识别位点Cac8I 识别位点ClaI识别位点CspCI 识别位点CviAII 识别位点CviKI-1识别位点CviQI 识别位点DdeI 识别位点DpnI 识别位点DpnII 识别位点DraI识别位点DraIII 识别位点DrdI 识别位点EaeI 识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI 识别位点EcoO109I识别位点EcoP15I 识别位点EcoRI 识别位点EcoRV 识别位点FatI 识别位点FauI 识别位点Fnu4HI 识别位点FokI 识别位点FseI 识别位点FspI 识别位点HaeII 识别位点HaeIIIHgaI 识别位点HhaI 识别位点HincII 识别位点HindIII 识别位点HinfI 识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IVHpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI 识别位点NaeI识别位点NarI识别位点NciI识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII 识别位点NlaIV 识别位点NmeAIII 识别位点NotI 识别位点NruI 识别位点NsiI 识别位点NspI 识别位点PacI识别位点PaeR7I 识别位点PciI 识别位点PflFI 识别位点PflMI 识别位点PhoI识别位点PleI识别位点PmeI 识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI 识别位点PvuI 识别位点PvuII 识别位点RsaI 识别位点RsrII识别位点SacI 识别位点SacII 识别位点SalI 识别位点SapI 识别位点Sau3AI 识别位点Sau96I 识别位点SbfI 识别位点ScaI 识别位点ScrFI识别位点SexAI 识别位点SfaNI 识别位点SfcI识别位点SfiI识别位点SfoI 识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI 识别位点SpeI 识别位点SphI识别位点SspI 识别位点StuI 识别位点StyD4I识别位点StyI 识别位点SwaI 识别位点Taq αI识别位点TfiI 识别位点TliI识别位点TseI 识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点与大家共享（非原创）。

酶切位点汇总
酶切位点，又称为限制性内切酶位点，是指DNA分子上特定的序列，这些序列是限制
性内切酶可以识别和切割的地方。

限制性内切酶是一种在细菌和其它生物中广泛存在的酶，能够切割或切除一个或多个DNA碱基对。

这些限制性内切酶在生物技术领域广泛应用，用
于DNA序列分析、DNA重组、基因工程等方面。

以下是常见的几种酶切位点：
1. EcoRI切割位点是5′-GAATTC-3′，这是一种广泛应用的限制性内切酶，通常用于DNA纯化、制备DNA载体等。

2. BamHI切割位点是5′-GGATCC-3′，BamHI能够切割链间，产生具有黏性末端的DNA 序列。

常被用于制备双链DNA的黏性末端。

4. PstI切割位点是5′-CTGCAG-3′，PstI是一种双切酶，可以切割成不同长度的DNA 序列，适用于构建多种不同长度的DNA分子。

总之，酶切位点及其对应的限制性内切酶在现代生物领域有着广泛的应用和重要的作用。

了解不同的酶切位点是有很大帮助的，它可以为实验设计和分子生物学研究提供基础。

同时，也让我们更好地理解限制性内切酶在DNA分子上的作用，帮助我们在生物技术领域
更加熟练地掌握其应用。

PCR引物设计时酶切位点的保护碱基表
不同内切酶对识别位点以外最少保护碱基数目的要求（在本表中没有列出的酶，则通常需在识别位点两端至少加上6个保护碱基，以确保酶切反应的进行。

）
注释
1.如果要加在序列的5’端，就在酶切位点识别碱基序列（红色）的5’端加上相
应的碱基（黑色），如果要在序列的3’端加上保护碱基，就在酶切位点识别碱基序列（红色）的3’端加上相应的碱基（黑色）。

2.切割率：正确识别并切割的效率。

3.加保护碱基时最好选用切割率高时加的相应碱基。

常用限制性内切酶酶切位点汇总Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点 KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI 识别位点MnlI识别位点MscI 识别位点MseI 识别位点MslI识别位点MspA1I识别位点MspI 识别位点MwoI识别位点NaeI识别位点NarI 识别位点NciI 识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII 识别位点NlaIV识别位点NmeAIII识别位点NotI 识别位点NruI识别位点NsiI识别位点NspI 识别位点PacI 识别位点PaeR7I 识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI 识别位点PmlI识别位点PpuMI 识别位点PshAI 识别位点PsiI识别位点PspGI识别位点PspOMI 识别位点PspXI识别位点PstI识别位点PvuI 识别位点PvuII 识别位点RsrII识别位点SacI 识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI 识别位点ScaI 识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI 识别位点SmlI识别位点SnaBI识别位点SpeI 识别位点SphI识别位点SspI识别位点StuI 识别位点StyD4I 识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI 识别位点TseI识别位点Tsp45I 识别位点Tsp509I 识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI 识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。

切割率%酶寡核苷酸序列2 hr 20 hrAcc I G GTCGAC C 0 0CG GTCGAC CG 0 0CCG GTCGAC CGG 0 0Afl III C ACATGT G 0 0CC ACATGT GG >90 >90CCC ACATGT GGG >90 >90Asc I GGCGCGCC >90 >90A GGCGCGCC T >90 >90TT GGCGCGCC AA >90 >90Ava I C CCCGGG G 50 >90CC CCCGGG GG >90 >90TCC CCCGGG GGA >90 >90BamH I C GGATCC G 10 25CG GGATCC CG >90 >90CGC GGATCC GCG >90 >90Bgl II C AGATCT G 0 0GA AGATCT TC 75 >90GGA AGATCT TCC 25 >90BssH II G GCGCGC C 0 0AG GCGCGC CT 0 0TTG GCGCGC CAA 50 >90BstE II G GGT(A/T)ACC C 0 10BstX I AACTGCAGAA CCAATGCATTGG 0 0 AAAACTGCAG CCAATGCATTGG AA 25 50CTGCAGAA CCAATGCATTGG ATGCAT 25 >90Cla I C ATCGAT G 0 0GATCGAT C 0 0CC ATCGAT GG >90 >90CCC ATCGAT GGG 50 50EcoR I G GAATTC C >90 >90>90 >90CG GAATTC CGCCG GAATTC CGG >90 >90Hae III GG GGCC CC >90 >90AGC GGCC GCT >90 >90TTGC GGCC GCAA >90 >90Hind III C AAGCTT G 0 0CC AAGCTT GG 0 0CCC AAGCTT GGG 10 75Kpn I G GGTACC C 0 0GG GGTACC CC >90 >90CGG GGTACC CCG >90 >90Mlu I G ACGCGT C 0 0CG ACGCGT CG 25 50Nco I C CCATGG G 0 0CATG CCATGG CATG 50 75Nde I C CATATG G 0 0CC CATATG GG 0 0CGC CATATG GCG 0 0GGGTTT CATATG AAACCC 0 0GGAATTC CATATG GAATTCC 75 >90GGGAATTC CATATG GAATTCCC 75 >90Nhe I G GCTAGC C 0 0CG GCTAGC CG 10 25CTA GCTAGC TAG 10 50切割率%酶寡核苷酸序列2 hr 20 hrNot I TT GCGGCCGC AA 0 0ATTT GCGGCCGC TTTA 10 10AAATAT GCGGCCGC TATAAA 10 10 ATAAGAAT GCGGCCGC TAAACTAT 25 90 AAGGAAAAAA GCGGCCGC AAAAGGAAAA 25 >90Nsi I TGC ATGCAT GCA 10 >90 CCA ATGCAT TGGTTCTGCAGTT >90 >90Pac I TTAATTAA 0 0G TTAATTAA C 0 25CC TTAATTAA GG 0 >90Pme I GTTTAAAC 0 0G GTTTAAAC C 0 25GG GTTTAAAC CC 0 50AGCTTT GTTTAAAC GGCGCGCCGG 75 >90Pst I G CTGCAG C 0 0TGCA CTGCAG TGCA 10 10 AA CTGCAG AACCAATGCATTGG >90 >90AAAA CTGCAG CCAATGCATTGGAA >90 >90CTGCAG AACCAATGCATTGGATGCAT 0 0Pvu I C CGATCG G 0 0AT CGATCG AT 10 25TCG CGATCG CGA 0 10 Sac I C GAGCTC G 10 10Sac II GCCGCGG C 0 0TCC CCGCGG GGA 50 >90Sal I GTCGAC GTCAAAAGGCCATAGCGGCCGC 0 0 GC GTCGAC GTCTTGGCCATAGCGGCCGCG 10 50G 10 75ACGC GTCGAC GTCGGCCATAGCGGCCGCGGAASca I GAGTACT C 10 25AAA AGTACT TTT 75 75Sma I CCCGGG 0 10CCCCGGG G 0 10CC CCCGGG GG 10 50TCC CCCGGG GGA >90 >90Spe I GACTAGT C 10 >90GG ACTAGT CC 10 >90CGG ACTAGT CCG 0 50CTAG ACTAGT CTAG 0 50Sph I G GCATGC C 0 0CAT GCATGC ATG 0 25ACAT GCATGC ATGT 10 50Stu I AAGGCCT T >90 >90GA AGGCCT TC >90 >90AAA AGGCCT TTT >90 >90Xba I CTCTAGA G 0 0GC TCTAGA GC >90 >90TGC TCTAGA GCA 75 >90CTAG TCTAGA CTAG 75 >90Xho I C CTCGAG G 0 0CC CTCGAG GG 10 25CCG CTCGAG CGG 10 75Xma I CCCCGGG G 0 0CC CCCGGG GG 25 75CCC CCCGGG GGG 50 >90TCCC CCCGGG GGGA >90 >90。

AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI 识别位点ApeKI 识别位点ApoI 识别位点AscI 识别位点AseI 识别位点AsiSI 识别位点AvaI 识别位点AvaII 识别位点AvrII 识别位点BaeI 识别位点BamHI识别位点BanI 识别位点BanII 识别位点BbsI 识别位点BbvCI 识别位点BbvI 识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsgI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI 识别位点BsmFI 识别位点BsmI 识别位点BsoBI 识别位点Bsp1286I 识别位点BspCNI 识别位点BspDI 识别位点BspEI 识别位点BspHI 识别位点BspMI 识别位点BspQI 识别位点BsrBI 识别位点BsrDI 识别位点BsrFI 识别位点BsrGI 识别位点BsrI 识别位点BssHII 识别位点BssKI 识别位点BssSI 识别位点BstAPI 识别位点BstBI 识别位点BstEII 识别位点BstNI 识别位点BstUI 识别位点BstXI 识别位点BstYI 识别位点BstZ17I 识别位点Bsu36I 识别位点BtgI 识别位点BtgZI 识别位点BtsCI 识别位点BtsI 识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI 识别位点EciI 识别位点EcoNI 识别位点 EcoO109I识别位点EcoP15I 识别位点EcoRI 识别位点EcoRV 识别位点FatI 识别位点FauI 识别位点Fnu4HI 识别位点FokI 识别位点FseI 识别位点FspI 识别位点HaeII 识别位点HaeIIIHgaI 识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IVHpyCH4V识别位点 KasI识别位点KpnI识别位点MboI 识别位点MboII 识别位点MfeI 识别位点MluI 识别位点MlyI 识别位点MmeI 识别位点MnlI 识别位点MscI 识别位点MseI 识别位点MslI 识别位点MspA1I 识别位点MspI 识别位点MwoI 识别位点NaeI 识别位点NarI 识别位点NciI 识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsaI识别位点RsrII识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点TaqαI识别位点TfiI识别位点TliI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点与大家共享（非原创）。

限制性内切酶酶切位点汇AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI 识别位点BfuAI 识别位点BglI 识别位点BglII 识别位点BlpI 识别位点Bme1580I 识别位点BmgBI 识别位点BmrI 识别位点BmtI 识别位点BpmI 识别位点Bpu10I 识别位点BpuEI 识别位点BsaAI 识别位点BsaBI 识别位点 BsaHI 识别位点BsaI 识别位点BsaJI识别位点BsaWI 识别位点BsaXI 识别位点BseRI 识别位点BseYI 识别位点BsgI 识别位点BsiEI 识别位点BsiHKAI 识别位点BsiWI 识别位点BslI 识别位点BsmAI 识别位点BsmBI 识别位点BsmFI 识别位点BsmI 识别位点BsoBI 识别位点Bsp1286I 识别位点BspCNI 识别位点BspDI 识别位点BspEI 识别位点BspHI 识别位点BspMI 识别位点BspQI 识别位点BsrBI 识别位点BsrDI识别位点BsrFI 识别位点BsrGI 识别位点BsrI 识别位点BssHII 识别位点BssKI 识别位点BssSI 识别位点BstAPI 识别位点BstBI 识别位点BstEII 识别位点BstNI 识别位点BstUI 识别位点BstXI 识别位点BstYI 识别位点BstZ17I 识别位点Bsu36I 识别位点BtgI 识别位点BtgZI 识别位点BtsCI 识别位点BtsI 识别位点Cac8I 识别位点ClaI识别位点CspCI 识别位点CviAII 识别位点CviKI-1识别位点CviQI 识别位点DdeI识别位点DpnI 识别位点DpnII识别位点DraI 识别位点DraIII识别位点DrdI 识别位点EaeI 识别位点EaeI 识别位点EagI 识别位点EarI识别位点EciI 识别位点EcoNI 识别位点EcoO109I 识别位点EcoP15I 识别位点EcoRI 识别位点EcoRV 识别位点FatI 识别位点FauI 识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IVHpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsaI识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TliI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点时间：2021.03.08创作：欧阳与。

Alul 识别位点5： . AG T CT ...T 3：. TCJSA...^Aflll 识别位点Acil 识别位点孑...此GC (3)p J . ..GGG^G. ,.5JAcll 识别位点$…从七US杠…TTGC^AA …hAlwl 识别位点5 ..GljATC<Nl7 3 3 . ,. C C T A G (N)，A 5'Acul 识别位点5. . CTGA AGfN), ▼…h3：」GACT TCfN)^.,. 5fAlwNl 识别位点e^XAGMN^TG,, 3 3：..GTC b MNNGAC (5)Afel 识别位点..AGCGCT .. .3P ..TCGCGA b ..5JApal 识别位点亍…GGGC 此.33\,CCCGGG...5JApaLl 识别位点3：..CAGGT i G...SAfllll 识别位点 片…M G R¥GT,・3b ： - .TG^RQA …5"ApeKl 识别位点F …GtwGC …可 3\.CGWCJ5.._5]Aatll 识别位点Agel 识别位点员・ ^/T CCGGT ^.O -3\. TGGCC^A ...S^Acc65l 识别位点*：・.GT W KAC 」■丁 P ：.. GA KMJG. ..5H Ahdl 识别位点5； ，G ACNN IvfNNGTC 3：..CTGNh!i NNNCAG (5)Accl 识别位点 *：・・ GTW KA C _・3 忆…GA KMJG. ..5Alel 识别位点5 ,TxACNffflNQTO- 7?3H 3 .GTGN\MNCAC 5"Bell识别位点Banl识别位点S^.CCRY^G.-.S^ Banll识别位点Bael识别位点Bcgl识别位点5：爲㈣CKgTGGW 3J 3：.屮㈣GUT(g2®N城” S BciVl识别位点5. ..GTATCCC帖3 . ,. C A 7 AGG(N}^. 5Apol识别位点&…P TA AT T J.y 3：.. YTTAA^R ..5JAscI识别位点S .^GG^GCGCC ・・・3 3J CCGCGCJ3G. ,.5JA Asel识别位点y…AfT AAT ■二住. T AATJA.,,5^AsiSI识别位点$： GRGGe -3”a^.c^ycGAG.-.s*Bbsl识别位点5. ,.GAAGAC(M).T.3" 3： ..CTTCTG{N}ti. . . 5^BbvCl识别位点亍…CtfTCAGC …IT 『…GGAG.CG…h『…GCGAT^GC..・3 p.. CGtyAGCG. . 5Aval识别位点Bbvl识别位点5 .GC AGC(M)D T. .3^ 3. . CGTCGiM^^-.S'CVCGRG…丁p；. G AGCY.C.,,5'Avail识别位点F…(SfeWGC二3" 『…ccwqp…hAvril识别位点忙，比TdGG ,一鼾..GGATGJD (5)Bccl识别位点5 .CCATC(N}f 3 3； .GGTAG(hl). 5ABceAl识别位点5. . . ACGGCIN)!* .3 3：…TGGCG(M)%f ^(fi>AC<N)J GTAYC㈣「3”P^(N)TG(N)4CATRG(叽圄BamHl 识别位点F…G%ATCC・・8 p：,.CCT AG^S.. .5'Bmtl 识别F …TG ATCA ・..3” P J ... ACTAGJ...5JBfal 识别位点&... <Tl AG ….3T 3：,.GAT ；C. ,.5JBfuAI 识别位点5 ACCTGC(N)7 3 :T ,.TGG ACG(N)… . . .5「ABgll 识别位点3 CGGI^NNNNCCG.. .5Bglll 识别位点罚 AT GT …丁 b …TCT*磬…甲Blpl 识别位点|5 …GC T TNAGC …IT 0- CG AN LCG .5'*Bme1580l 识别位点IS. ..GKGCM't , ..3_P^. . <^MCGKG .6BmgBl 识别位点|5\ ,.CAC T GTC.,.3J 忙…GTOf AO …hBmrl 识别位点. ACTGGG(N} T y3： .. TGACCC(N),^..^5；.,,GCTAGC,.・宙 3：. CJ J ATCG...5*Bpml 识别位点5：…CTGEAG(W …3" 3 . ..GACC1. .5'Bpu10l 识别位点酣…GC T TFJ AGC ■…丁3GG Ahl 匚8 …5，BpuEl 识别位点5. t CTTGAQ(M)，7.3 3. .. G AACTC(M),^ hBsaAl 识别位点5； , YAC^GTR, ..3' 3： RT(\CAY. . 5^BsaBl 识别位点5； ,G^TNN>JNATC, -33J . CTANNJNNTAG .5'BsaHl 识别位点…GFTtG¥C 、・.3‘ :T.KYGQRG …5’Bsal 识别位点5r . .GGTCTCCM)/ 33. ^CCAGAGCM)^. . ,5HBsaJl 识别位点5：.任NNGG …了3 GGN忖Cf …5「Bmtl识别BsmA I 识别BsaXI 识别位点b J ..\(N)AC(N)^CTCC(«)|, 3y . /NjTGfN^GAGGCN). . 5rBseRI 识别位点|5\ .G AGGAG(N)r T .富 p j . ,.CTCCTC(N)lk ..5-BseYI 识别位点 厂氏G g C …3 卜…GGGT^p …£Bsgl 识别位点..GTGCAG(N}(7 3 3 . . C ACGTciN},^. , .5BsiEI 识别位点CGRv'fcG. .3 3 GCJfRGC. ,5JBsiHKAI 识别位点5：..GWGCWt ・・ + 3" 茁…妄WCGWG.…扩BsiWI 识别位点 耳讯 T R CG 773P'.. GC ATfi/：…h5： . GTCTC (谢一…才 3 . . .C AG AG Nh ■…亍BsmBI 识别位点5 ..CGTCTC(N)7 .3^ 3 . ..GCAGAG (NL ^ …5rBsmFI 识别位点亍…GGG AC^N)，7 -3* 書…CCCTG^^. .,5rBsmI 识别位点k ；lh 0AATQCN T \3 T.. XTT ACGN■BsoBI 识别位点F …CGG4G …丁 3：..GRGC^C..5JBsp1286l 识别位点^TTGDGCrffc.rTr3： 一 ・ qHGGDG …5’BspCNI 识别位点5., .CTCAG(N)T 5 ,.y anf1 & ..CTCAGCNJ/7 3： . .G AG TC (N)^ _.53. . ,GAGTCfN>_ . 5_BspDI 识别位点t ；,T AftGAT (ip 3^TAGCJA.-.S^BslI识别位点P：, CC NN NWhT(4NGG. ..3^ 3d GGNN NNNMNCC . ..5F BspEI识别位点5；.. ffcCGGA …丁BssKI识别位点BstZ17l识别位点|5•…ft A T G A . h3^(3： . . AGTAGJ . . 5JBspMI识别位点MCCTGC(N)/. -T4TGG ACG(N\,^,.^ BspQI识别位点S\..GCTCTTC*^T..r S：.XS AGAAG(N^..&'BsrBI识别位点5 - cc(stfcr^33J. .. GGC^AG..・hBsrDI识别位点5 GC AATGHN T,,3^}…CGTTAqNN …hBsrFI识别位点fc；. R'tCGGV..^' 护…YGGcqp…yBsrGI识别位点|sT7 f^ifACA f +.3l p：-, AGATGJ ..5 ・BsrI识别位点S：. . AC TGG . 3*3：. TG ACJ3N …hBssHII识别位点p：. R CNGG…;r3：.i GGNCq t...5,BssSI识别位点S…WZGZ、、S3：_GTGG冬…5’BstAPI识别位点5；. r GCAMNNbTNlGC ,, 3J 3J,..CGTN L NNNNACG....5,JkBstBI识别位点F…TTtGAA …3" 卜…AAGCJT..6BstEII识别位点y…Gt TH AG G …丁扩……5’BstNI识别位点匚ftfwGG …丁3'. GG\\CC. 5^BstUI识别位点GGtG,…丁3：.,GqpCJ …5’BstXI识别位点6；, .CCANNMN^NTGG.T.a 3. .GGTN^NMNNNACC 5BstYI识别位点BssKI识别位点BstZ17l识别位点E:「F T GATCJ■了卜…¥0"初…亍BssKI识别位点BstZ17l识别位点p•…GT"T AC…科A…W冉TO…5"Bsu36l识别位点^.-CCfTNAGG...3rBtgl识别位点Is；.. (7c RY Ge...3" 卜…GG¥RQCBtgZI识别位点p：一GC(3 ATG(N】「33 ..CGC TAC(N)…k..5^BtsCI识别位点p•…GSGTG Wd p\.CGTC AC^hlN,. 5Btsl识别位点p•...G C A G TG 卜...CGTCghIN (5)Cac8l识别位点5•…GC砾IGC…丁『…CG卧X…割Clal识别位点5\ .. AT'feGAT , .,3TAGCJA (51)CspCl识别位点p- ：ori(N)CAA(N^GTGG{N>i£l^ 3「(3\^.lJ(N)GTT(N)：CACC(N)1frr;. 5*t： ~C^TG ,..3* 買…GT也p…h CviKl-1识别位点S...RC7CY ..*3J …5’CviQl识别位点5：. (frAC …:T3’…C仲…亍Ddel识别位点(7T N A G…3「…GANT卫….亍Dpnl识别位点CH,I；5 G A T C 3^3」CT AG . 5"rC%Dpnll识别位点F" t n T C…雷『…G T A £\一-亍Dral识别位点TT A …丁3 ■…AAAJTT …5’Dralll识别位点『…CACNMNtTG …丁扩…GTG*N 忖NCAG. hDrdl识别位点5\ GACNNNN T N NGTC ,3'3\.CTGNNNNNNCAG. ,.5JCviAll识别位点EcoRV 识别位点fc ；.. CCTNI^NNAGG,.,3^ p ；. r GG AN NNJN NTCC 5^EcoO109lHaell 识别位点F …RGCGE-丁 常…空GCGFt …时b ； , , AT TC ,-3 卜…GTTA Af …亍Eael 识别位点|5：…丹 GCCR,3 p\.RCCG<5^. .5'Eael 识别位点|5 ...丹 GCCR,S 卜...RCCGGy (5)Eagl 识别位点fc. V&GCCG 31 P ： . GCGGGJC...5'Earl 识别位点5V T ：GA TX TC 7731 3■…CT AJAG …品Fatl 识别位点F …tATG …3「GTAC A ...5JFaul 识别位点犷…CCCGC (N)7. . .3* ，GGGCGtN\^ ,5「Fnu4HI 识别位点6；.. GCTNGC ・・4 3 .. GGN£G (5)识别位点&…RG3CC¥・・：$Fspl 识别位点5；., TG<ft5CA,, 3EcoP15l 识别位点|5\ .C AGCAGfN^^ 3 p ： ..GTCGIC(N)^. ,.5^HaelllEcoRl 识别位点Hgal 识别位点5 .GACGC (N )5T .3.CTCTTC(N)7 GAG AAG(N\Ecil 识别位点 B . GGC GG A(N)n T .3" 3 仁.CCGCCTeO 注…h Fokl 识别位点5 GG ATG(M)…T .3^ 3；. .CCT AC{M)nk ..5^Fsel 识别位点EcoNl 识别位点5 …GGCCGG 七 C …3一 3： . CCJ J GGCGG ..5*3. . ,CTGCG{M)，k ..5-EcoRV识别位点Hhal识别位点$...GCG七 (3)3. .. QPGG ,.5MHin cll识别位点杖…GTYlH AC…3 歯…GA蔺TG…寸Hin dlll识别位点5；r. A>GCT T・、・3”『…TTCG《…5"Hinfl识别位点F…G鼻NTC…3"P' . C7 . 5"HinP1l识别位点航GG…了b：.tGqp …5*Hpal识别位点|5；.GTr^AC,-3'P：.. CA AJTG.-.5Hpall识别位点$...此GG・・S 卜...GGqp (5)Hphl识别位点6+<. GGTGA ㈣3 忙…GGACT 叫血*6Hpy188l识别位点TC N'GA…3”p j.., A C T …5”Hpy188lll识别位点亍…TCfNNGA…歹31.鼻GNN卫T..hHpy99l识别位点3'..1-h GCWGC. ,5・HpyAV识别位点5...,CCTTCtN)7，. 3* 3.r GGAAG(N)t..亍HpyCH4lll识别位点5；., ACN^T . .*3. , rt^NC A . . 5 HpyCH4lVHpyCH4\识别位点TGt A…3"3J AC fc GT...5JKasl识别位点亍…代CgC…丁3..4CCGCGp....5* Kpnl识别位点6；GGT AC T C f,■歹C£ATGG.…印Mboll识别位点Mbol识别位点SvT^MTrc …歹A…GTAG「HMboll识别位点MspAII 识别位点Nhel 识别位点 Mspl 识别位点3■…GGqC …5’Mlul 识别位点Nael 识别位点Mmel 识别位点Msel 识别位点賓…F T A A …科3：…―匸T …引NgoMIV 识别位点了…GtCGGC ■二歹3：「GGGUQT.Gfi* . G A AG A (N)J .3 P ：, + CTTCT (N)7A . + ,5*Mfel 识别位点，…內ATTG …3”& ..GT TA A 卫…£Mwol 识别位点6；. . GCNNNNNT M NG C ・・.33J . . CGNN^NN NHNCG ..5Mlyl 识别位点G AGTC (rj ；7 了 :,CTCA G(N)「…5* h …GC 比GC …3「3：..CGG t CCG.…5JNarl 识别位点『，G 比GCG …丁3： . CCGqGG.^5*Mnll 识别位点5： .,CCTC 侔人二 3” $…GSG 讯厂$Ncil 识别位点&…CC T SGG .,3即…GGS^CC. ..5-Mscl 识别位点5： ,. TGGtCA^.S 3 ... ACCpGT ...5JNcol 识别位点5： ., Ct ATGG , , a^-GGTACp . . .5*Ndel 识别位点5 ..ATQ .3^3：..GT AlAC …5’Msll识别位点5 ...CMNNllNRTG (3)3 GTANN^NNY A C 5MspAII识别位点Nhel识别位点PshAI 识别位点 PaeR7l 识别位点Nlalll 识别位点 石GAT 贰+・玄 扩…少T AC "Pcil 识别位点 F U TGT …扩3 . ..TGTA^A.^5'NotI 识别位点TAGC, ..3p ：・"CCGg •3眷…CGATQG 佔3\ GAGCI ；C. ..5*NmeAIII 识别位点5"…GC CG AG 呱二3PflMI 识别位点5；.,CCANNNN T NT&G <1,3 3：「GGT 轉NNNACC ・.，5'PhoI 识别位点5…GGtG …b$…GCGGCCGC …3 fa ：.. CGGCGG_CG..,5^NruI 识别位点TCbfcG 昌…3 P ：.. AGCpGT ..5PleI 识别位点S ；. . GAGTC IN)T 3 3：., CTCAG (N),A .,.5NsiI 识别位点$…ATGC 肝一团 卜….1；ACGTA .,5'PmeI 识别位点$…GTTT 工冉AG.S 3J ..CAAAJTTG..J 5^NspI 识别位点$…RCAI ■計…tr b ■…百GTMR一$PacI 识别位点b J ... TT AA'Tr AA・「3P J ... A A TJ AA TT (5)PmlI 识别位点CAC'GTG..,3 3： .GTC^CAC . 5JPpuMI 识别位点6；tl RG T GWC CY.^3^ 3： ..YCC W GjGR .. .5F GG 晶g …歹6：..GAGhTNN<5TC ,B …CC 轉GG …h S^.CTGNN^NGAG .NlalV 识别位点PflFI 识别位点P^.CTGNN A NNCAG...5*Psil识别位点胃…TTkTAA,■罔卜…从公T T…因PspGI识别位点卩…丸CWGG…y 卜…GGWCQ…5"PspOMI识别位点|5：・・bGGCCC：・h 忙…CGgcys…hPspXI识别位点6：.. V<fTCGAG6 ...3H 3 .BGAGCT；CV r5^Pstl识别位点$…CTGG帀…3". G^A0GT C .5・Pvul识别位点$…CGiATtG …丁P J...GCJAGC.,.5* Pvull识别位点5：CA^TG.,3-击...GTQJ3AG (5)Rsal识别位点b：.7GT\C...3l 卜…gjTd…戲Rsrll识别位点卧…C西WCCG…歹，…GCCWGjpg…扩Sacl识别位点时…GAGCe…丁扩…CJCGAG.^S^Sacll识别位点6；., GCQd^G,…y 3：.. GG^CGGC -.S^Sall识别位点<fT C G A C. *. 3^ 3J...CAGCT；G...5JSapl识别位点5：…GCTCTTC 啊人=*3「3「・CGAGAAG{Nhv5”Sau3AI识别位点b；..T GATC .313：.. GT AG^ ..5*Sau96l识别位点亍…品NCC…丁3\NG J p...5JSbfl识别位点3」GGf CGTGC …5”Seal识别位点5；, t AGT^CT < ,r3TCAJGA ...S^ ScrFI识别位点SnaBI 识别位点 Tfil 识别位点歛：・TAC'feTA .・.歹3： .. ATGJSAT .. S'Spel 识别位点5：二 Xt TAGT …丁 3J ... TGATC^A .-S^Sphl 识别位点GC ATGfc …3「 『…qpTACG …亍Sspl 识别位点6： ., AA T>TT , 3J ... TTAJAA...5JStul 识别位点AGC^CCT ••謁 3： . TCqSGA …hStyD4l 识别位点6：JCGNGG …孑 3’…GGNCq . SStyl 识别位点E ：・，GPWWGG,・・：3”GGWWC&—WSwal 识别位点6*,.. A T T iTi A A T r ,. 3 3： ..T A A Aj T TA (5)Taqa I 识别位点■…fbGA …;r b ： ■ • A Gb -CCfhGG . .3 p J . ..GGN^CC •.生SexAl 识别位点，…心GWGGT …了3\..TGGWCqA (5)SfaNI 识别位点fe ： . . GC A JC (N)J. ,3' 忙 GGTAG (N)；>1 + ,5*Sfcl 识别位点F …疔TRYAG …：T 卜…—ysSfil 识别位点b:. .GGCCNNNr7NGGCC. ..3H p.-CCGGN^NWUCCGG .5Sfol 识别位点S^.GGC'teCC,-^' 3 …CCI^pG 右•一一5"SgrAI 识别位点p •…CF?t CGG¥G …丁 p\.GYGGGC^RC .S^Smal 识别位点『…右右右gUC …hSmll 识别位点b, ,C T T Y RAG …3 P . r ,G AH YT^C .. 5Tlil 识别位点Xhol 识别位点(7TCGAG.-・33；.. G A G C T^C (5)Tsel 识别位点Xmal 识别位点賢…衣CGGG …丁3■…GGGgC …5’Xmnl 识别位点橫一上T£直匚…3",G AANN >IMTTC ,p ； ..CASTG^3：. XT TN N^N MA AG < …5"Zral 识别位点 $…X ATT …wfcC. GAC\STC ・.3rf(5... TT AA M ..5J 3：. CTGJCAG.. .5"TspMl 识别位点 $...比CGGG (3)$…GGGCC£…节与大家共享（非原创）6；ANNNNN^NNNTGG...3J 3； GGTNNNh^NNNNNACC-.STspRl 识别位点|5；-.NNCASTGNM T -.3d忸.&NNGTSACMN …5"Tthllll 识别位点6： , ,G AC hfNNGTC,-・旷 3\.CTGNN i NCAG...5^Xbal 识别位点ftT AGA (3)P ； .. AG A TCJ (5)Tsp45l 识别位点 Tsp509l 识别位点Xcml识别位点SnaBI识别位点Tfil识别位点。