Rabbit Anti-Antithrombin III抗体说明书

兔抗人胸腺细胞免疫球蛋白【药品名称】通用名称：兔抗人胸腺细胞免疫球蛋白英文名称：Rabbit Anti-human Thymocyte Immunoglobulin【成份】本品主要成分：兔抗人胸腺细胞球蛋白每小瓶5ml含25mg兔抗人胸腺细胞球蛋白辅料：甘氨酸、氯化钠、甘露醇。

注射用水。

【适应症】1.移植用免疫抑制剂：预防和治疗器官排异反应。

治疗激素耐受和移植物抗宿主病（GvHD）。

2.血液学疾病：治疗再生障碍性贫血。

【用法用量】用量根据不同的适应症选择剂量，建议参考剂量为：器官移植的免疫抑制治疗：预防器官排异：肾脏、胰腺、肝脏移植后1.25-2.5毫克/kg/日，1至3周，心脏移植后3-10日。

终止用药时，无需逐渐减量。

治疗器官排异：2.5-5毫克/kg/日，至临床症状消失和生物学指标改善。

再生障碍性贫血：2.5-5毫克/kg日，连续5日。

此产品用于治疗再障的临床实验暂缺。

激素耐受和急性移植物抗宿主病治疗：剂量【不良反应】滴注兔抗人胸腺细胞免疫球蛋白时和用后有如下副反应的报道：全身性副反应为寒战、发热、心跳过速、呕吐和呼吸困难。

局部副反应有输液处局部疼痛及末梢血栓性静脉炎的报道。

罕见迟发性过敏反应，如初次使用后7-15日，可能会发生血清病（发热、搔痒、皮疹伴有关节痛）。

速发严重过敏反应极为罕见。

常见和极严重的副反应发生在第一次滴注后。

有些副反应的发生机理是与细胞分裂释放有关。

应用皮质醇和抗组胺制剂进行预防治疗并减慢滴速或增加稀释液量（等渗0.9%生理盐水或5%葡萄糖溶液）可降低或减轻【禁忌】急性感染时，禁用免疫抑制治疗。

对兔蛋白或本品其他成分过敏者。

【注意事项】兔抗人胸腺细胞免疫球蛋白必须住院并在严密监控状态下使用。

有些严重副反应可能与滴速有关。

应严格执行使用方法中提示的滴速要求。

输药期间必须自始至终严密监控患者。

由于可能发生血清病，应向接受兔抗人胸腺细胞免疫球蛋白治疗者说明。

如果发生副反应，减慢滴速或中断滴注至症状缓解。

免疫兔制备抗体流程英文回答：Preparation of Antibodies in Immunized Rabbits.The process of producing antibodies in immunizedrabbits involves several key steps:1. Immunization: The rabbit is injected with the antigen of interest, which triggers an immune response. The antigen can be a protein, peptide, or other substance that the body recognizes as foreign.2. Antibody Production: In response to the antigen, the rabbit's immune system produces antibodies that arespecific to the antigen. These antibodies are produced by B cells, which are a type of white blood cell.3. Collection of Serum: Once the rabbit has produced a sufficient level of antibodies, blood is collected from therabbit. The serum, which contains the antibodies, is then separated from the blood cells.4. Purification of Antibodies: The serum is thenpurified to isolate the antibodies of interest. This can be done using various techniques, such as affinity chromatography or protein A/G purification.5. Characterization of Antibodies: The purified antibodies are characterized to determine their specificity, affinity, and other properties. This is important for ensuring that the antibodies are suitable for theirintended use.中文回答：免疫兔制备抗体流程。

Product InformationMonoclonal ANTI-FLAG® M2, Clone M2 Produced in Mouse, Affinity Isolated AntibodyF1804Storage Temperature −20 °CProduct DescriptionMonoclonal ANTI-FLAG® M2 is a mouse-derived, affinity-purified IgG1 monoclonal antibody that binds to fusion proteins containing a FLAG® peptide sequence.1 The M2 antibody will recognize a FLAG® peptide sequence at the N-terminus,Met-N-terminus, C-terminus, or internal sites of a fusion protein. Binding of the M2 antibody is not dependent on calcium.Monoclonal ANTI-FLAG® M2 is useful for detection, identification, and capture of fusion proteins that contain a FLAG® peptide sequence by common immunological procedures, such as Western blotting, immunofluorescence, and immunoprecipitation. ReagentSupplied at a concentration of ∼ 1 mg of protein per mL of solution, and formulated in 50% glycerol,10 mM sodium phosphate, and 150 mM NaCl,pH 7.4. The formulation contains no antimicrobial preservatives.Antigenic binding siteN-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C SpecificityThe monoclonal antibody detects only the target protein band(s) on a Western blot from an E. coli, plant or mammalian crude cell lysate.SensitivityThe monoclonal antibody detects as little as 2 ng of target protein by dot blot. The Western blot is tested down to 10 ng, but may detect lower using the procedure detailed below. Precautions and DisclaimerFor R&D use only. Not for drug, household,or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.Preparation InstructionsImmediately prior to use, dilute the monoclonal antibody in Tris-buffered saline (TBS), pH 8.0, with 3% nonfat milk (Cat. No. T8793). Dilutions in the described procedures are provided as guidelines. Adjust the antibody concentration to maximize detection sensitivity and minimize background. StorageStore the undiluted antibody at −20 °C in working aliquots. The product, as formulated, will not freeze when stored at the recommended temperature. Note: Over time, small amounts of purified antibodies can precipitate due to intermolecular hydrophobic interactions. If precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in most applications.ProceduresWestern Blot Immunostaining2-5Note: this procedure is based on chemiluminescent detection using Chemiluminescent Peroxidase Substrate-1 (Cat. No. CPS160). Dilutions of both primary and secondary antibodies may require optimization when using other substratesor conditions.1.Separate fusion proteins containing aFLAG® peptide sequence from sample lysatesusing a standard SDS-PAGE protocol. Load2.5–10 µg of total protein lysate per lane.2.Transfer proteins from the gel to a nitrocellulosemembrane, Immobilon®-P, or otherpolyvinylidene difluoride (PVDF) membrane. ThePVDF membrane may provide greaterdownstream sensitivity.3.Wash the blot in at least 0.5 mL/cm2 of purifiedwater for 2–3 minutes employing gentle agitation (50–60 rpm).4.Block the blot with at least 0.5 mL/cm2 ofTris Buffered Saline (TBS), pH 8.0, with 3%nonfat milk for 30 minutes at room temperature, employing gentle agitation.5.Remove the blocking agent and wash once with0.5 mL/cm2 of Tris buffered saline, pH 8.0(Cat. No. T6664).6.Add the desired concentration of monoclonalantibody to the blot. A final antibodyconcentration of 1 µg/mL (1:1,000 dilution of the antibody as supplied) in at least 0.5 mL/cm2 ofTBS with 3% nonfat milk is suggested. Incubateat room temperature for 30 minutes employinggentle agitation.Note: Dilutions must be optimized for differentsubstrates and systems.7.Decant off the Monoclonal ANTI-FLAG® M2solution and wash once with at least 0.5 mL/cm2of TBS, pH 8.0.8.Add the secondary antibody in the form ofAnti-Mouse IgG-Peroxidase (Cat. No. A9044) orequivalent. The concentration of secondaryantibody must be optimized based on thesubstrate being used. For detection usingChemiluminescent Peroxidase Substrate-1, a final secondary antibody dilution of 1:30,000 should be employed. Specifically, it is suggested theantibody as supplied be diluted in at least0.5 mL/cm2 of TBS with 3% nonfat milk. Incubatethe blot employing gentle agitation at roomtemperature for 30 minutes. 9.Wash the blot at least three times for a total of15 minutes (5 minutes per wash) in TBS with0.05% TWEEN® 20, pH 8.0 (Cat. No. T9039).Agitate gently, employing at least 0.5 mL/cm2 ofwash solution.10.Develop the blot using ChemiluminescentPeroxidase Substrate-1 or an equivalent reagentfor 5 minutes. Do not agitate the blot during this incubation step. Drain briefly and wrap inplastic film.11.Expose BioMax® Light film to the blot for a rangeof times from several seconds up to 10 minutes.It is recommended that a quick exposure of10–30 seconds be performed to determine theoptimal exposure time needed. If the signal is too intense even at the short exposure times, allowthe signal to decay over a 1–8 hour period(or longer if necessary), and then re-exposethe film.Indirect ImmunofluorescentCytochemical StainingMonoclonal ANTI-FLAG® M2 may be utilized in immunocytochemical staining procedures when used in conjunction with a labeled secondary antibody (indirect).6 A generic procedure for adherent cell staining is described, using immunofluorescence, employing an Anti-Mouse IgG-FITC conjugate asthe label.1.Grow and transfect cells on coverslips.2.Fix the cells by incubation with phosphatebuffered saline, pH 7.4 (Cat. No. P3813),containing 4% paraformaldehyde (Cat. No.P6148), and 4% sucrose (Cat. No. S1888) for15 minutes at room temperature.3.Wash the fixed cells with PBS for 5 minutes.Repeat once.4.Permeabilize the cells by incubation with 0.25%TRITON™ X-100 (Cat. No. T9284) in PBS for5 minutes.5.Wash the cells with PBS for 5 minutes.Repeat once.6.Block by incubation with 10% bovine serumalbumin (Cat. No. A9647) in PBS (10% BSA/PBS) for 30 minutes at 37 °C.7.Incubate with Monoclonal ANTI-FLAG® M2 dilutedin the range of 1:500 to 1:2,000 in 3% BSA/PBSfor 2 hours at 37 °C.8.Wash with PBS for 5 minutes. Repeat twice.9.Incubate with the secondary antibody, Anti-MouseIgG- FITC (Cat. No. F9137) at a 1:1,000 dilutionin 3% BSA/PBS for 45 minutes at 37 °C.10.Wash with PBS for 5 minutes. Repeat twice.11.Mount coverslips with cells side down on glassslides using a small drop of mounting mediumsuch as polyvinyl alcohol for semi-permanentmounting. The inclusion of an anti-fading agentlike DABCO® in the mounting medium(25–100 mg/mL, for example Cat. No. 10981) isstrongly recommended. Seal coverslips to glassslides (with nail polish, for instance).12.Examine by fluorescence microscopy. FITC has anabsorption maximum at 492 nm with an emission maximum at 520 nm. Immunoprecipitation (IP)Monoclonal ANTI-FLAG® M2 may be used in IP procedures when used in conjunction with an insoluble carrier matrix, such as a Protein G resin. Alternatively, EZview™ Red Protein G Affinity Gel (Cat. No. E3403) or the Protein G Immunoprecipitation Kit (Cat. No. IP50) may be used. EZview Red ANTI-FLAG® M2 Affinity Gel(Cat. No. F2426) or ANTI-FLAG® M2 Affinity Gel (Cat. No. A2220) may be utilized directly for IP. See reference 5 for general protocols.Enzyme Immunoassay (EIA)Monoclonal ANTI-FLAG® M2 may be used in EIA procedures. Typically, a fusion protein containing a FLAG® peptide sequence is directly adsorbed (or otherwise presented) within the wells of a multiwell polystyrene plate. The Monoclonal ANTI-FLAG® M2 antibody may be diluted up to 1:50,000 for subsequent incubation within the plate wells. Detection may be accomplished using Anti-Mouse IgG-Peroxidase (Cat. No. A9044) or equivalent, diluted 1:10,000, followed by an appropriate substrate for visualization.We also offer the ANTI-FLAG® High Sensitivity, M2 coated 96-well plate (Cat. No. P2983) for EIA-based screening applications. References1.Brizzard, B.L., et al., Immunoaffinity purificationof FLAG® epitope-tagged bacterial alkalinephosphatase using a novel monoclonal antibodyand peptide elution. BioTechniques, 16(4):730-735 (1994).2.Bjerrum, O.J., and Heegaard. N.H.H., CRCHandbook of Immunoblotting of Proteins, Volume I, Technical Descriptions. CRC Press(Boca Raton, FL), pp. 229-236 (1988).3.Dunbar, B.S. (ed.), Protein Blotting: A PracticalApproach. IRL Press (New York, NY),pp. 67-70 (1994).4.Fortin, A., et al., A 56- to 54-kilodalton non gratasignal in immunoblot analysis using thehorseradish peroxidase chemiluminescencesystem. Biochem. Cell Biol., 72(5-6):239-243 (1994).5.Harlow, E., and Lane, D., Antibodies: ALaboratory Manual. Cold Spring Harbor Laboratory Press (Cold Spring Harbor, NY) (1988).6.Ciaccia, A.V., and Price, E.M., IBI FLAG Epitope,1: 4-5 (1992)..Troubleshooting Guide (Western Blot Immunostaining Procedure) Problem Possible Cause SolutionFusion protein is not detected. Protein is notexpressed.Verify nucleic acid sequence and reading frame of the FLAG® fusionprotein in vector construct. If sequence is present, attempt tooptimize expression.Target proteinpoorly representedin sample.Positive controls (10 ng/lane recommended) should always beincluded. If the positive control works, the sample may not containthe FLAG® fusion protein of interest, or it may be present atconcentrations too low to detect. Immuno-precipitation withANTI-FLAG® M2 Affinity Gel (Cat. No. A2220) may be required forlow concentrations of FLAG fusion proteins.Positive controls we have available:•Amino-terminal FLAG-BAP™ Fusion Protein (Cat. No. P7582).•Carboxy-terminal FLAG-BAP™ Fusion Protein (Cat. No. P7457).•Amino-terminal Met-FLAG-BAP™ Fusion Protein (Cat. No. P5975).Defective detectionreagentsRun appropriate controls to ensure performance. Use 10 ng/lane of acontrol FLAG-BAP™-fusion protein as a positive control. If no signal isobtained with the control, repeat the procedure using a fresh lot ofsecondary antibody-HRP conjugate, along with freshly preparedreagents.Inadequateexposure timeusingchemiluminescentsystem.If no signal is observed on the film, expose for longer times. It isrecommended to try exposure times ranging from about 5 seconds toas long as 10 minutes.Inappropriate film Switch to film designated for chemiluminescent detection such asBioMax® Light.No target proteinpresent onmembrane.Verify transfer onto the membrane by visualizing proteins usingPonceau S solution (Cat. No. P7170). If possible, a positive controlshould always be run to ensure that the detection systemcomponents are functioning normally. Pre-stained protein markers(for example, Cat. No. C1992 or C4861) may also be used to verifycomplete transfer of proteins from gel to membrane.Antigen is coveredby blockingreagent, due tooverblocking.Masking of a signal can occur if the blocking reagent (such as caseinor gelatin containing blocking buffers) is used at an excessively highconcentration. A dilution ranging from 1:1 to 1:3 may be performedto decrease the concentration of blocking reagent. If the problempersists, use TBS with 3% nonfat milk (Cat. No. T8793).Antibodyconcentration isnot optimal.Determine the optimal working dilution for the MonoclonalANTI-FLAG® M2 antibody via titration. Consider using a higherconcentration of antibody, if no signal or a weak signal is detected.Also, antibody used at an excessively high concentration can causesignal inhibition, especially in chemiluminescent detection systems.The life science business of Merck operatesas MilliporeSigma in the U.S. and Canada.Merck and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates.All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.Problem Possible Cause SolutionHigh non-specific background Cellular extract concentration istoo high.2.5–10 µg of total lysate protein perlane is usually enough to obtain agood signal. Load less cellularextract or serially dilute the cellularextract to determine the optimalsignal to noise ratio.Monoclonal ANTI-FLAG® M2antibody concentration is too high.Dilute the Monoclonal ANTI-FLAG®M2 antibody to a concentrationranging from 0.1–0.5 µg/mL. UseTBS with 3% nonfat milk asthe diluent.Secondary antibody cross-reactivity.For the secondary antibody, it isrecommended that users initiallyemploy dilutions of 1:30,000.Higher dilutions may be necessary,or a more specific secondaryantibody should be used.Monoclonal ANTI-FLAG® M2antibody cross-reacts with naturallyoccurring FLAG®-like epitopes.Increasing the temperature to37 °C during the blocking, binding,and wash steps may reducecross-reactivity. Lysates frommock-transfected controls(transfected with plasmid lackinginsert DNA) will help distinguish theFLAG®-fusion proteins from othercross-reacting proteins.NoticeWe provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose.The information in this document is subject to change without notice and should not be construed as a commitment by the manufacturing or selling entity, or an affiliate. We assume no responsibility for any errors that may appear in this document.Technical AssistanceVisit the tech service page at /techservice.Standard WarrantyThe applicable warranty for the products listed in this publication may be found at /terms. Contact InformationFor the location of the office nearest you, go to /offices.。

兔子Ⅲ型前胶原肽(PⅢNP)ELISA试剂盒使用说明书Elisa kit规格：48孔配置/96孔配置标准品稀释液：1.5ml×1瓶酶标试剂：3 ml×1瓶（48）/6 ml×1瓶（96）【兔子Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA试剂盒elisa试剂盒】本试剂仅供研究使用计算：以标准物的浓度为横坐标，OD值为纵坐标，在坐标纸上绘出标准曲线，根据样品的OD值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的直线回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实际浓度。

试剂盒组成：封板膜:2片（48）/2片（96）说明书:1份密封袋:1个标准品：2700ng/L 0.5ml×1瓶0.5ml×1瓶2-8℃保存酶标包被板: 1×48 1×96 2-8℃保存样品稀释液: 3ml×1瓶 6 ml×1瓶2-8℃保存显色剂A液: 3ml×1瓶 6 ml×1瓶2-8℃保存显色剂B液: 3ml×1瓶 6 ml×1瓶2-8℃保存终止液: 3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液:（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存实验原理：本试剂盒应用双抗体夹心法测定标本中兔子Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA试剂盒水平。

用纯化的兔子Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA试剂盒抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA试剂盒，再与HRP标记的Ⅲ型前胶原肽(PⅢNP)lisa试剂盒说明书LISA 试剂盒抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。

碧云天生物技术/Beyotime Biotechnology 订货热线：400-168-3301或800-8283301 订货e-mail ：****************** 技术咨询：***************** 网址：碧云天网站 微信公众号HLA-DR Rabbit Monoclonal Antibody产品编号 产品名称包装 AF2065HLA-DR Rabbit Monoclonal Antibody50µl产品简介：来源 用途交叉反应性 分子量 RabbitWB, IF, IHC, ICC, FCH, M, R29 kDaWB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.配套提供了Western 一抗稀释液，可以用于Western 检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：抗体详细信息如下:About this AntibodyName HLA-DR Rabbit Monoclonal AntibodyCategory Rabbit Monoclonal Antibody (RabMAb); Primary antibody Isotype IgGPurification Affinity purification About the ImmunogenImmunogen Recombinant protein Gene ID 3122(Human) SwissProt P01903(Human) Synonyms HLA-DRA1; MLRWCategoryImmunology/InflamationBackgroundMajor histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC II α/β dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (β-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.包装清单：产品编号 产品名称包装 AF2065 HLA-DR Rabbit Monoclonal Antibody50µl AZ050 Western 一抗稀释液50ml —说明书1份保存条件：WBIP IFIHC ICCFCELISA ChIP 1:1,000-1:5,000 -1:50-1:2001:50-1:2001:50-1:2001:50-1:100--2 /3 AF2065 HLA-DR Rabbit Monoclonal Antibody400-1683301/800-8283301 碧云天/BeyotimeHLA-DR Rabbit Monoclonal Antibody -20ºC 保存，Western 一抗稀释液-20ºC 或4ºC 保存，一年有效。

Polymer HRP * Goat Anti Rabbit IgG(H+L)【产品货号】RS0010【产品名称】1、通用名称：Polymer HRP羊抗兔二抗即用型（免疫组织化学）2、英文名称：Polymer HRP * Goat Anti Rabbit IgG(H+L)【包装规格】类别规格工作液10mL/瓶、100mL/瓶【预期用途】免疫组织化学染色，配合Immunoway常规兔来源的浓缩型或即用型一抗使用。

【作用原理】数个过氧化物酶分子和数个二抗分子结合在同一多聚体上构成了一种简单而灵敏的显色系统【主要组成成份】磷酸盐缓冲液0.01mol/LGoat anti Rabbit HRP 大于0.05mg/L【储存条件及有效期】1、储存要求：2℃～8℃密封保存。

2、有效期：一年。

【样本要求】新鲜活检或外科样本组织，甲醛固定，固定时间8-24小时要求取材、脱水、石蜡包埋并制成蜡块。

配合Immunoway常规浓缩型或即用型一抗使用。

【检验方法】1、人体组织样本先经甲醛固定。

2、固定后的组织经过脱水等一系列操作制备成蜡块。

3、用切片机把组织蜡块切成切片。

4、手工操作免疫组化实验。

常规推荐步骤如下：4.1. 将需要进行实验的组织切片取出，插入玻片架上后放入60-65℃的烤箱中，烤片1-1.5h。

4.2. 将切片置于以下染色缸中梯度反应：二甲苯10分钟×2次二甲苯5分钟无水乙醇5分钟×2次95%乙醇5分钟85%乙醇5分钟PBS清洗3分钟×3次4.3. 抗原修复（方法见一抗供应商，或采用常规高压、微波、酶修复等）4.4.根据组织大小直接滴加内源性过氧化物酶阻断剂到组织上，通常为100uL, 直至完全覆盖组织。

室温10分钟，PBS清洗3分钟×3次。

此步骤通常在水浴法、酶法等温和抗原修复后加入。

或者组织本身含有较多内源性过氧化物酶中加入。

4.5. 根据组织大小滴加一抗到组织上，通常为100uL,直至完全覆盖组织。

抗人T细胞兔免疫球蛋白Kang Ren T Xibao Tu MianyiqiudanbaiAnti-human T Lymphocyte Rabbit Immunoglobulin本品系由人T淋巴细胞免疫家兔后,取其血清或血浆经去除杂抗体、纯化、浓缩后,再经病毒灭活处理并加入适宜稳定剂后冻干制成。

不含防腐剂和抗生素。

1 基本要求生产和检定用设施、原料及辅料、水、器具、动物等应符合“凡例”的有关要求。

2 制造2.1 免疫血清2.1.1 免疫抗原免疫用抗原为人胸腺细胞,或符合“血液制品生产用人血浆”中供血浆者标准的健康人血液分离的人淋巴细胞。

胸腺供体为HBsAg、HCV抗体、HIV-1/HIV-2抗体和梅毒血清学检查应为阴性。

分离后T淋巴细胞数应不低于总细胞数的90%,红细胞数应不高于总细胞数的5%。

2.1.2 免疫动物免疫用家兔至少应符合普通级实验动物的要求(附录ⅩⅢ B和附录ⅩⅢ C),体重为2000~2500克,检疫合格者方可使用。

2.1.3 免疫方法按批准的免疫程序免疫。

2.1.4 采血及分离血清加强免疫后,淋巴细胞毒试验效价达1∶400时即可采血。

分离的血清置-20℃以下保存。

保存期不应超过2年。

2.2 原液2.2.1 混合血清经56℃水浴30分钟灭能,辛酸-硫酸铵盐析分离纯化或经批准的其他分离纯化法,杂抗体吸收,再用DEAE-sephadex A-50色谱纯化抗人T细胞兔免疫球蛋白。

杂抗体吸收用的人红细胞、人血小板、人胎盘组织及人血浆的供给者应符合“血液制品生产用人血浆”中供血浆者标准。

2.2.2 原液检定按3.1项进行。

2.3 半成品制备2.3.1 配制加入适量麦芽糖或其他适宜稳定剂。

按成品规格以灭菌注射用水稀释至所需蛋白质浓度,并适当调整pH值及钠离子浓度。

2.3.2 半成品检定按3.2项进行。

2.4 成品制备2.4.1 分批应符合“生物制品分批规程”规定。

2.4.2 分装及冻干应符合“生物制品分装和冻干规程”规定。

禽流感特效产品-治疗禽流感-注射药-精制卵黄抗体III型产品特点：（1）本品为精制三联卵黄抗体冻干粉，经过浓缩提纯、外源性病菌灭活，具有效价高、安全性高、稳定性强的特点（2）抗禽流感、新城疫抗体（IgY）为：血凝抑制效价HI大于11log2 ，抗传支抗体（IgY）为：血凝抑制效价HI大于9log2。

专家提示：注射本品时可跟囊素三肽或植物血凝素-禽专用与头孢噻呋钠同时使用，效果更佳！【兽药名称】商品名：精制卵黄抗体III型通用名：精制卵黄抗体英文名：Refined yolk antibody【性状】本品为微黄色晶体状冻干粉。

【药理作用】本品为高效抗病毒药物，抑制病毒核酸合成，并诱导机体产生内源性干扰素，促进抗体形成，阻止病毒复制增殖，从而起到抗感染抗病毒杀菌作用，增加免疫球蛋白的分泌量，调节免疫功能。

本抗体富含的特异性免疫球蛋白（IGg）可迅速中和并清除血液中特定病毒，使病毒丧失侵染性，直接抑制病毒在机体内的扩散和增殖，同时清除各组织器官内的病毒，阻碍病毒在机体细胞内的复制，协助机体排除病毒，从而从根本上使机体摆脱病毒侵害，恢复正常功能。

【功能主治】本品主要防治家禽的流感病毒H9型、H5型、H7型以及H5型的变异毒株，传支，新城疫等急、慢性病毒性疾病。

其临床症状表现为初期体温升高，精神沉郁，叫声减小，缩颈，嗜睡，采食量减少或急骤下降，渴欲增加、羽毛纷乱无光、甩鼻、嗉囊空虚，排黄绿色稀便，个别鸡拉白色稀便，泄殖腔周围被白色粪便污染。

呼吸困难，咳嗽，打喷嚏，张口呼吸。

后期部分家禽只有神经症状，表现头颈向后仰，抽搐，运动失调，瘫痪等。

蛋禽感染后，蛋壳质量变差、畸形蛋增多，会出现软壳蛋、无壳蛋、褪色蛋等。

解剖可见病鸡鼻腔、气管内有淡黄色黏液，支气管有黄色干酪样分泌物,并伴有针尖大出血点；肺脏小支气管周围有灰红色肺炎灶，气囊混浊增厚；肾脏肿大，呈灰白色花斑状，有尿酸盐沉积；肾小管和输尿管扩张。

卵泡的充血和液化很明显，另外还有输卵管水肿和炎性分泌物。

【名称】抗凝血酶-Ⅲ【英文名】antithrombin Ⅲ【别名】【概述】人体内与凝血系统功能相拮抗的是抗凝血系统，在正常情况下，两者保持动态平衡，从而维持血液在血管内的正常流动。

抗凝血酶Ⅲ是抗凝系统中最重要的成分，它由肝脏合成，为一种多功能的丝氨酸蛋白酶抑制物，可抑制凝血酶生成，具有强大的抗凝活性．占血浆抗凝酶活性的70%。

【原理】在含有抗AT-Ⅲ抗血清(抗体)的琼脂糖凝胶板孔中，加入受检血浆或血清(抗原)，进行电泳，抗原和抗体会形成特异地火箭样免疫沉淀峰。

沉淀峰的高度与样品中AT-Ⅲ的抗原量成正比关系。

【试剂】(1)兔抗人AT-Ⅲ血清。

(2)巴比妥-盐酸缓冲液(pH值8.2)：巴比妥钠47.6g，溶于3L水中，加1.17mol/L盐酸(浓盐酸193ml加水1807ml)55ml，测pH值，并以盐酸校正pH 值至8.2，然后用水补足总量至4265ml。

(3)标准血浆。

(4)4.4mmol/L磷钼酸溶液。

【操作方法】(1)将溶解后的兔抗人AT-Ⅲ血清(试剂A)按瓶签标示稀释倍数的一半，用电泳缓冲液稀释备用。

另用水配成20g/L浓度的琼脂糖溶液。

将上述两种溶液在56℃等量混匀，倾入夹有80mm×80mm×1.5mm“U”形模框的两块玻板中，待凝固后，于板之一侧打一排加样孔，孔径2mm，孔距3mm。

(2)将溶解后的标准血浆(试剂B)用水作1∶2、1∶4、1∶8、1∶16的稀释，样品也用水作1∶5稀释，然后用微量加样器分别定量加入加样孔，每孔5μl，于15V/cm电势梯度下，电泳6h(加样孔应置于负极侧)。

(3)电泳完毕，将凝胶板置4.4mmol/L磷钼酸溶液中，固定15min，量取各火箭峰，自加样中心至峰尖的高度(mm)。

以不同稀释度标准血浆的峰高(X)对相应的AT-Ⅲ抗原含量(Y)在正坐标纸上制作标准曲线。

再将样品的峰高在标准曲线上直接读出AT-Ⅲ抗原含量；亦可作直线回归运算，得回归公式Y＝b x＋a，将样品的峰高代入式中x，即得出ATⅡ∶Ag的含量Y。

E-mail:Website: 2-step plus Poly-HRP Anti Rabbit/Mouse IgG Detection SystemCat. No: E-IR-R211Size: 3 mL/ 6 mL/ 18 mL产品编号产品名称 3 mL 6 mL 18 mL Storage E-IR-R211A 3% H2O2 3 mL 6 mL 18 mL 2~8°CE-IR-R211B Polymer Helper (Ready-to-Use) 3 mL 6 mL 18 mL 2~8°CE-IR-R211C Polyperoxidase-anti-Mouse/Rabbit IgG (Ready-to-Use) 3 mL 6 mL 18 mL 2~8°C说明书一份产品简介Elabscience 公司最新推出的二步法免疫组化试剂盒，将二抗的单价Fab 片段和过氧化物酶HRP 聚合在一起，替代传统方法中的二抗和三抗，直接放大抗原抗体结合的信号，既保留了抗体特异性结合抗原的能力，又可有效地避免聚合分子过大而造成的空间位阻。

与传统的SP三步法相比，此试剂盒具有简单、快速、敏感等特点，试剂盒不再使用生物素，避免了由于内源性生物素所造成的背景染色。

本产品可用于检测一抗是Rabbit和Mouse来源的单克隆抗体或多克隆抗体，本品独特的聚合物辅助剂有利于大分子的检测系统更好地结合所检测的一抗分子。

此检测系统是目前已知的灵敏度最高的免疫组化检测系统。

使用说明1.脱蜡、水化组织切片。

2.根据所应用的抗原抗体要求，对组织切片进行抗原修复。

3.加E-IR-R211A 3% H2O2，孵育10分钟，以阻断内源性过氧化物酶。

4.PBS或TBS 冲洗，3分钟×3次。

5.滴加适当稀释的一抗，室温或37°C孵育1~2小时或4°C过夜（后37°C复温30 min），PBS 或TBS冲洗，3分钟×3次。

抗凝血酶ⅲ octapharma ab 说明书抗凝血酶Ⅲ Octapharma AB 说明书一、通用名称：抗凝血酶Ⅲ Octapharma AB二、成份：本产品主要成分为抗凝血酶Ⅲ（Antithrombin III），其它辅助成分有胶体、缓冲剂等。

三、性状：本品为无色或略带黄色的透明液体。

四、适应症：抗凝血酶Ⅲ Octapharma AB 用于以下情况：1. 高凝血状态的患者；2. 血友病患者在手术或出血并发症风险较高的情况下；3. 普通肝功能不全的患者。

五、规格和剂型：本品规格为500 IU和1000 IU。

剂型为注射用制剂。

六、用法和用量：1. 高凝血状态的患者：根据患者体重和输注前抗凝血酶Ⅲ的水平，推荐的剂量为每次0.3-0.5 IU/ kg体重，每天1次。

注射速度不得超过每分钟0.2 IU/kg体重。

2. 血友病患者：根据手术类型和患者需要，推荐的剂量为每次0.5-1 IU/ kg体重，每天1次。

注射速度不得超过每分钟0.2IU/kg体重。

3. 肝功能不全的患者：推荐的剂量为每次0.5-1 IU/ kg体重，每天1次。

注射速度不得超过每分钟0.2 IU/kg体重。

七、适应症禁忌症：1. 对本品成分过敏者禁用；2. 过敏体质者禁用。

八、注意事项：1. 使用本品前要检查患者是否对本品成分过敏，如有过敏症状需立即停用。

2. 使用本品应密切注意患者的凝血状态，如有出血或血栓并发症，应及时调整剂量或停止使用。

3. 本品不得与其他药物混合使用，特别是含有金属离子的药物。

4. 孕妇和哺乳期妇女应在医生指导下使用本品。

九、不良反应：使用本品后可能出现以下不良反应：1. 过敏反应，包括皮疹、荨麻疹、呼吸困难等。

2. 出血或血栓并发症。

3. 肌肉痛、关节痛。

十、贮藏：本品应贮存在温度不超过25°C的阴凉干燥处。

十一、包装：本品包装为玻璃制剂针管或注射器。

十二、有效期：本品有效期为24个月。

十三、生产企业：抗凝血酶Ⅲ Octapharma AB 产品由XXX公司生产。

兔抗人血清白蛋白使用方法兔抗人血清白蛋白使用方法人血清白蛋白作为血浆容量扩充剂用途广泛，是当前的研究热点之一。

人血清白蛋白（Human Serum Albumin，简称HSA）是人血浆中的蛋白质。

在体液中人血清白蛋白可以运输脂肪酸、胆色素、氨基酸、类固醇激素、金属离子和许多治疗分子等：同时维持血液正常的渗透压。

在临床上人血清白蛋白可用于治疗休克与烧伤，用于补充因手术、意外事故或大出血所致的血液丢失，也可以作为血浆增容剂。

二抗和一抗的区别：第一抗体就是平常所说的抗体，即能和抗原特异性结合。

第二抗体是能和抗体结合的，即抗体的抗体。

主要用于检测抗体的存在。

一抗是针对抗原的抗体，二抗是针对一抗的抗体。

即抗体也可以充当抗原刺激机体产生抗体。

也就是说，抗原进入机体刺激机体免疫系统产生免疫应答，由B细胞可以产生与相应抗原发生特异性结合的特殊蛋白质。

一抗二抗都是一种可以特异结合别的物质的基团，而且一抗可以至少结合两种其他基团（底物和二抗）。

中文名称：兔抗人血清白蛋白英文名称：Polyclonal Rabbit Anti-HSA蛋白浓度：5mg/ml包含成分：0.01M PBS、PH:7.2、N稳定剂制备方法：高纯度HSA经过多次免疫健康雄性白兔而得来，免疫血清双扩散效价不低于1：32纯化方法：HSA亲和纯化交叉反应：没有记录产品纯度：以和HSA反应的抗体计不低于总蛋白的95%储存方法：请-20℃避光保存注意事项：避免反复冻融，如防腐剂对实验有影响，可以透析去除。

透析后冷冻会降低效价。

有效期限：请您再管体注明有效期内使用。

Background: PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phospha-tase (2). The main substrates of PTEN are inositol phospho-lipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382 and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein coupled signaling during chemotaxis (9,10).Specificity/Sensitivity: PTEN Antibody detects endog-enous levels of total PTEN protein. The antibody does not cross-react with related proteins.Source/Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide correspond-ing to residues in the carboxy-terminal sequence of human PTEN.Background References:(1) C antley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.(2) M yers, M.P . et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.(3) M yers, M.P . et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.(4) W an, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.(5) W u, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.(6) V azquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.(7) T orres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.(8) F reeman, D.J. et al. (2003) Cancer Cell 3, 117-30.(9) F unamoto, S. et al. (2002) Cell 109, 611-23.(10) I ijima, M. and Devreotes, P . (2002) Cell 109, 599-610kDa PTENAktL N C a P N I H /3T 3P C 3C 6C O S -7200140100806050403020806050H e L aWestern blot analysis of extracts from various cell lines usingPTEN (D4.3) XP ® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).W, IP , IHC-P EndogenousH, M, R, Mk, (C)54 kDaRabbit**Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.*Species cross-reactivity is determined by western blot.** A nti-rabbit secondary antibodies must be used to detect this antibody.Recommended Antibody Dilutions: Western Blotting 1:1000 Immunoprecipitation 1:50 Immunohistochemistry (Paraffin) 1:125† Unmasking buffer: CitrateAntibody diluent: SignalStain ®Antibody Diluent #8112 Detection reagent: SignalStain ® Boost (HRP , Rabbit) #8114 †Optimal IHC dilutions determined using SignalStain ® Boost IHC Detection Reagent.For application specific protocols please see the web page for this product at .Please visit for a complete listing of recommended companion products.Applications Species Cross-Reactivity*Molecular Wt.Isotype IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.Entrez-Gene ID #5728 Swiss-Prot Acc. #P60484Species Cross-Reactivity Key:H —humanM —mouseR —ratHm —hamsterMk —monkey Mi —minkC —chickenDm —D. melanogaster X —XenopusZ —zebrafishB —bovineDg —dog Pg —pig Sc —S. cerevisiae All —all species expected Species enclosed in parentheses are predicted to react based on 100% homology.Applications Key:W —Western IP —Immunoprecipitation IHC —Immunohistochemistry ChIP —Chromatin ImmunoprecipitationIF —ImmunofluorescenceF —Flow cytometryE —ELISA E-P —ELISA PeptideImmunohistochemical analysis of paraffin-embedded human colon using PTEN (D4.3) XP ® Rabbit mAb.© 2012 C e l l S i g n a l i n g T e c h n o l o g y , I n c .X P ® a n d C e l l S i g n a l i n g T e c h n o l o g y ® a r e t r a d e m a r k s o f C e l l S i g n a l i n g T e c h n o l o g y , I n c .This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.Orders n 877-616-CELL (2355) orders@ Support n 877-678-TECH (8324) info@ Web n © 2009 C e l l S i g n a l i n g T e c h n o l o g y , I n c .Immunohistochemical analysis using PTEN (D4.3) XP ® Rabbit mAb on SignalSlide(TM) PTEN IHC Controls#8106 (paraffin-embedded LNCaP (left) and NIH/3T3 (right) cells).。

多发性获得性易栓症的实验诊断摘要目的对多发性肠系膜静脉栓塞患者及其家系进行相关检查和基因诊断，分析其所患易栓症类型以及与遗传的相关性。

方法对该家系7人用发色底物法对蛋白C活性（PC:A）、抗凝血酶活性（AT:A）和蛋白S活性（PS:A）分别进行检测，用Western blot 检测血浆中的抗凝血酶含量和分子量，用Trizol法提取患者及家系成员外周血全基因组DNA，对抗凝血酶的7个外显子及其侧翼序列使用PCR扩增，用直接测序法对患者的AT扩增产物进行测序分析和基因突变分析。

结果该患者（II2）的AT含量正常, AT:A为49%，经AT基因序列的突变分析未发现突变位点，其他检测结果正常；患者弟弟（II3）AT:A为45%，其他检查结果正常。

该家系其他成员的PC:A 、PS:A 、AT:A及AT含量均正常。

结论根据易栓症相关检测结果及基因序列突变分析，患者与其弟弟有车祸外伤史（分别为髂骨骨折和肋骨骨折）等获得性诱因存在，该两患者不存在AT基因突变，诊断为获得性易栓症。

关键词易栓症，获得性易栓症，肠系膜静脉栓塞1965年Egeberg O.在报道一个家族性抗凝血酶(antithrombin，AT)缺陷症时第一次提出易栓症（thrombophilia）一词。

易栓症是指有发生静脉血栓栓塞症倾向的一类疾病，据有无遗传性倾向和有无获得性因素存在，可分为遗传性易栓症和获得性易栓症。

获得性易栓症是获得性因素导致的易栓症，常见诱因有创伤或有手术史、长时间止动、年龄增加、妊娠期或产褥期以及长期口服避孕药或激素等；还有一些疾病同样容易诱发易栓症，如：获得性抗凝蛋白缺乏、抗磷脂综合症、获得性凝血因子升高、肾病综合症、糖尿病等。

而遗传性易栓症是存在常染色体显性家系遗传的易栓症，伴有相关蛋白的基因突变，常见有蛋白S缺陷、抗凝血酶缺陷、蛋白C缺陷、FV Leiden突变和凝血酶原20210A突变等。

国内研究报道的易栓症家系仍不多，本研究拟以一个易栓症家系进行研究，分析其抗凝血酶活性、蛋白C活性和蛋白S活性等进行表型诊断，检测其基因序列进行基因突变分析，以确定其所患易栓症的类型，并探讨基因诊断在易栓症类型诊断中的应用。

CD MARKER ANTIBODIESThe cluster of differentiation (abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules. So-called CD markers are routinely used for the immunophenotyping of cells. Despite this use, they are not limited to roles in the immune system and perform a variety of roles in cell differentiation, adhesion, migration, blood clotting, gamete fertilization, amino acid transport and apoptosis, among many others. As such, Proteintech’s mini catalog featuring its antibodies targeting CD markers is applicable to a wide range of research disciplines.IntroductionImmunohistochemical of paraffin-embedded human hepatocirrhosis using PECAM1, CD31 Figure 1: Immunofluorescence staining of PECAM1 (11256-1-AP), Alexa 488 goatImmunohistochemical staining of paraffin-embedded human colon tissue using NCAM1 Mouse brain tissue lysate was separated by SDS PAGE, followed by Western blot experiment Immunohistochemical of paraffin-embedded human colon using E-cadherin Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FAS Antibody (13098-1-AP) at a dilution of 1:50 (10x objective).Immunohistochemical of paraffin-embedded human skin using E-cadherinHeLa cells were subjected to SDS PAGE followed by Western blot with CD44 antibody Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD44Immunohistochemical of paraffin- embedded human kidney using CD146/MCAM Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using CD146/OPTIMIZE YOUR ANTIBODY EXPERIMENTS WITH PROTEINTECHRequest your free technical guide from .THE BENCHMARK IN ANTIBODIESSince the day it was founded, Proteintech has been makingall of its products to the highest standards possible whilst takingcomplete responsibility for the quality of each product.• Proteintech makes every single antibody in its 12,000+ catalog.• Each Proteintech product is unique and cannot be bought undera different label.• Antibodies are tested with siRNA treated samples todemonstrate specificity.• It works in every single species and application or get a fullmoney-back refund.Proteintech has over 12,000 antibodies in its extensivecatalog, all fully validated and available for next day delivery.CD No.Antibody NameCat. No.TypeApplicationsCD10MME/Neprilysin 18008-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD10MME/Neprilysin 10302-1-AP Rabbit Poly ELISA, FC, IHC, WBCD10MME/Neprilysin 23782-1-AP Rabbit Poly ELISA, FC, IHC CD10MME/Neprilysin 23898-1-AP Rabbit PolyELISA, FC, IF, WBCD10MME/Neprilysin 60034-3-Ig MouseMono ELISA, IHC, WBCD102ICAM210121-2-AP Rabbit Poly ELISA, IHC, WB CD104Integrin beta-421738-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD105Endoglin/CD10510862-1-AP Rabbit Poly ELISA, IHC, IP , WBCD106VCAM-111444-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD107a LAMP121997-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD107a LAMP155273-1-AP Rabbit Poly ELISA, IF, WB CD109CD10918715-1-AP Rabbit Poly ELISA, IP , WB CD112Nectin 210100-2-AP Rabbit Poly ELISA, FC, IF, WB CD113PVRL3, Nectin 311213-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD114 G-CSFR 18310-1-AP Rabbit Poly ELISA, FC, WB CD116CSF2RA/CD11618307-1-AP Rabbit Poly ELISA, IHC, WB CD117c-Kit/CD11718696-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD118LIFR 22779-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD119IFNGR110808-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD11b CD11B/Integrin alpha M 20991-1-AP Rabbit Poly ELISA, FC, IHC, WB CD11bCD11B/Integrin alpha M21851-1-APRabbit PolyELISA, IHCCD No.Antibody NameCat. No.TypeApplicationsCD11c CD11c/Integrin alpha X 17342-1-AP Rabbit PolyELISA, FC, IHC, WBCD11c CD11c/Integrin alpha X 60258-1-Ig MouseMono ELISA, IHC, WBCD11d CD11d/Integrin alpha D 55162-1-AP Rabbit Poly ELISA, WB CD120a TNFR121574-1-AP Rabbit PolyELISA, FC, IF, IHC, WBCD120a TNFR160192-1-Ig Mouse Mono ELISA, FC, IHC, WB CD120b TNFR219272-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD121b IL1R213591-1-AP Rabbit PolyELISA, WBCD121b IL1R260262-1-Ig MouseMono ELISA, IF, WBCD122IL2RB 13602-1-AP Rabbit Poly ELISA, WB CD123IL3RA 13655-1-AP Rabbit Poly ELISA, WB CD125IL5RA 12655-1-AP Rabbit Poly ELISA, IF, IHC, WBCD126IL6R 23457-1-AP Rabbit Poly ELISA, IP , WB CD127CD127/IL7R 17626-1-AP Rabbit Poly ELISA, FC, IP , WB CD128CXCR155450-1-AP Rabbit Poly ELISA, IP , WB CD13CD1314553-1-AP Rabbit Poly ELISA, FC, IHC, WBCD132IL2RG 11409-1-APRabbit Poly ELISA, FC, WB CD133CD13318470-1-APRabbit Poly ELISA, FC, IF, IHC, WB CD133CD13318495-1-AP Rabbit Poly ELISA, IHC, WB CD133CD133-1, 2, 4, 719945-1-AP Rabbit Poly ELISA, IHC, IP , WBCD133CD133-1, 219946-1-AP Rabbit Poly ELISA, IP , WB CD134CD134/OX4020006-1-APRabbit PolyELISA, FC, WBA NTIBODY PRODUCT LIST233621117232111210CD No.Antibody NameCat. No.TypeApplicationsCD135FLT321049-1-AP Rabbit Poly ELISA, IP , WB CD136RON, MST1R 11053-1-APRabbit Poly ELISA, WB CD138CD138/ Syndecan-110593-1-APRabbit PolyCoIP ,Dot Blotting, ELISA, IF, IHC, WBCD138CD138/ Syndecan-160185-1-Ig MouseMonoELISA, WBCD138CD138/ Syndecan-160185-2-Ig MouseMono ELISA, IHC, WBCD14CD1417000-1-AP Rabbit PolyELISA, FC, IF, IHC, WBCD14CD1460253-1-Ig Mouse Mono ELISA, IF, IHC, WB CD140b PDGFRB13449-1-AP Rabbit Poly ELISA, FC, WB CD141Thrombomodulin 14318-1-AP Rabbit Poly ELISA, FC, IHC, WBCD146CD146/MCAM 17564-1-AP Rabbit PolyELISA, FC, IF, IHC, IP , WBCD146CD146/MCAM 66153-1-Ig Mouse Mono ELISA, FC, IHC, WB CD147CD14711989-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD148PTPRJ 55123-1-AP Rabbit Poly ELISA, IHC, WB CD15FUT419497-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD150SLAM/CD15010949-2-AP Rabbit Poly ELISA, WB CD151CD15110418-1-AP Rabbit Poly ELISA, IP , WB CD154CD15416668-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD156b ADAM17-Specific 20259-1-AP Rabbit Poly ELISA, IHC, WB CD159a NKG2A 10935-1-AP Rabbit Poly ELISA, IHC, WB CD164CD16412083-2-AP Rabbit Poly ELISA, FC, IHC, WBCD166ALCAM 21972-1-AP Rabbit Poly ELISA, IHC, WB CD167DDR110536-1-AP Rabbit Poly ELISA, IHC, WB CD168HMMR-Specific 15820-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD170SIGLEC513230-1-APRabbit PolyELISA, WBCD No.Antibody NameCat. No.TypeApplicationsCD172a SIRP alpha 14482-1-AP Rabbit Poly ELISA, WB CD172b SIRP BETA 111811-1-AP Rabbit Poly ELISA, IHC, WB CD172g SIRP gamma 11857-1-AP Rabbit Poly ELISA, WB CD181CXCR155450-1-AP Rabbit Poly ELISA, IP , WB CD182CXCR220634-1-AP Rabbit PolyELISA, IF, IHC, IP , WBCD183CXCR3B-specific 60065-1-Ig Mouse Mono ELISA, FC, IF, IHC, WB CD184CXCR411073-2-AP Rabbit PolyELISA, IP , WBCD184CXCR460042-1-Ig MouseMono ELISA, FC, WBCD192CCR2-specific 16153-1-AP Rabbit Poly ELISA, FC, IHC, WBCD195CCR517476-1-AP Rabbit Poly ELISA, FC, WB CD197CCR755425-1-AP Rabbit Poly ELISA, FC, IHC CD1a CD1A 17325-1-AP Rabbit PolyELISA, WBCD1a CD1A 60173-1-Ig MouseMono ELISA, WBCD2CD210299-1-AP Rabbit PolyELISA, FC, IHC, WBCD2CD260005-2-Ig MouseMonoELISA, IHC, WBCD20CD2060271-1-Ig Mouse Mono ELISA, FC, IHC, WB CD201EPCR 11391-1-AP Rabbit Poly ELISA, IHC, WB CD202b Tie219157-1-AP Rabbit Poly ELISA, IP , WB CD206CD20618704-1-AP Rabbit Poly ELISA, FC, IHC, WB CD207Langerin 11841-1-AP Rabbit Poly ELISA, WB CD208LAMP312632-1-AP Rabbit Poly ELISA, IF, IHC, IP , WB CD210a IL10RA 13356-1-AP Rabbit Poly ELISA, FC, WB CD210b IL10RB 15102-1-AP Rabbit Poly ELISA, WB CD210b IL10RB 19387-1-AP Rabbit Poly ELISA, WB CD212IL12RB113287-1-APRabbit PolyELISA, IF, IP , WB3253311421411371111128Integrin beta-4More validation images available on our website.CD133CD14CD2CD No.Antibody Name Cat. No.Type ApplicationsCD213a2IL13RA211059-1-AP RabbitPoly ELISA, IF, IHC, WBCD215IL15RA16744-1-AP RabbitPoly ELISA, IHC, WBCD22CD2266103-1-Ig MouseMono ELISA, IHC, WBCD220INSR20433-1-AP RabbitPoly ELISA, IP, WBCD221IGF1R-Specific20254-1-AP RabbitPoly ELISA, IHC, IP, WBCD222IGF2R-Specific20253-1-AP RabbitPoly ELISA, FC, IF, IHC, IP, WBCD223LAG-316616-1-AP RabbitPoly ELISA, IP, WBCD225IFITM111727-3-AP RabbitPoly ELISA, FC, IF IHC, IP, WBCD225IFITM1-Specific60074-1-Ig MouseMono ELISA, FC, IF, IHC, IP, WBCD226CD22617842-1-AP RabbitPoly ELISA, IHCCD227MUC119976-1-AP RabbitPoly ELISA, FC, IHC, IP, WBCD227MUC123614-1-AP RabbitPoly ELISA, FC, IHC, WBCD228MFI210428-1-AP RabbitPoly ELISA, WBCD23CD2318642-1-AP RabbitPoly ELISA, FC, IHC, WBCD23CD2360208-1-Ig MouseMono ELISA, IHC, WBCD23CD2360208-2-Ig MouseMono ELISA, IHC, WBCD230PrP12555-1-AP RabbitPoly ELISA, FC, IHC, IP, WBCD231TSPAN718695-1-AP RabbitPoly ELISA, IHC, IP, WBCD233band 318566-1-AP RabbitPoly ELISA, IHC, IP, WBCD234DARC55185-1-AP RabbitPoly ELISA, IHC, WBCD235a Glycophorin A15874-1-AP RabbitPoly ELISA, FC, IHC, WBCD24CD2418330-1-AP RabbitPoly ELISA, WBCD24CD2410600-1-AP RabbitPoly ELISA, IHC, WBCD244CD24416677-1-AP RabbitPoly ELISA, IHCCD247CD24712837-2-AP RabbitPoly ELISA, FC, IHC,IP, WBCD No.Antibody Name Cat. No.Type ApplicationsCD248TEM118160-1-AP RabbitPolyELISA, FC, IF,IHC, WBCD249ENPEP17655-1-AP RabbitPoly ELISA, FC, IFCD253TRAIL17235-1-AP RabbitPoly ELISA, IF, IP, WBCD254RANKL23408-1-AP RabbitPolyELISA, IHC, IP,WBCD255TNFSF1212537-1-AP RabbitPoly Not TestedCD256TNFSF1310680-1-AP RabbitPolyCoIP, ELISA, IF,WBCD257TNFSF13B11862-1-AP RabbitPoly ELISA, WBCD258TNFSF1414194-1-AP RabbitPoly Not TestedCD26DPP410940-1-AP RabbitPolyELISA, IF, IHC,WBCD261DR424063-1-AP RabbitPoly ELISA, WBCD262DR5-Specific15497-1-AP RabbitPoly ELISA, WBCD264DcR216781-1-AP RabbitPoly ELISA, IHC, WBCD267TNFRSF13B21520-1-AP RabbitPoly ELISA, WBCD27CD2711121-1-AP RabbitPoly ELISA, FC, WBCD271p75NTR55014-1-AP RabbitPolyELISA, FC, IF,IHC, IP, WBCD274PD-L1/CD27417952-1-AP RabbitPolyELISA, IF, IHC,IP, WBCD275ICOSLG14922-1-AP RabbitPoly ELISA, WBCD276CD27614453-1-AP RabbitPolyELISA, FC, IHC,WBCD278ICOS13338-1-AP RabbitPoly ELISA, WBCD279PD-1/CD27918106-1-AP RabbitPolyELISA, FC, IF,IHC, WBCD283TLR317766-1-AP RabbitPoly ELISA, FC, IHCCD284TLR419811-1-AP RabbitPoly ELISA, IHC, WBCD286TLR622240-1-AP RabbitPoly ELISA, WBCD29Integrin beta-112594-1-AP RabbitPoly ELISA, WBCD292BMPR1A12702-1-AP RabbitPoly ELISA, IF, IP, WBIL15RACD23TEM1RANKLMore validation images available on our website.22411232211821482131411121CD No.Antibody NameCat. No.TypeApplicationsCD295LEPR 20966-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD298A TP1B311142-1-AP Rabbit Poly ELISA, IHC, WB CD299CLEC4M 22003-1-AP Rabbit Poly ELISA, IHC, WB CD301CLEC10A/CD30113590-1-AP Rabbit Poly ELISA, WB CD303CLEC4C 17941-1-AP Rabbit PolyNot TestedCD304Neuropilin 160067-1-Ig Mouse Mono ELISA, IF, IHC, WB CD31CD3111265-1-AP Rabbit PolyELISA, FC, IF, IHC, IP , WBCD31CD3166065-1-Ig Mouse Mono ELISA, IF, IHC, WB CD316IGSF814387-1-AP Rabbit Poly ELISA, IP , WB CD317BST213560-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD318CDCP112754-1-AP Rabbit Poly ELISA, FC, WB CD319SLAMF712905-1-AP Rabbit Poly ELISA, WB CD320CD32010343-1-AP Rabbit Poly ELISA, FC, IHC, WB CD322JAM212972-1-APRabbit Poly ELISA, WB CD324E-cadherin 20874-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD325N-cadherin 13769-1-AP Rabbit Poly ELISA, IF, IHC, WBCD325N-cadherin 22018-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD326EPCAM 21050-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD327SIGLEC613454-1-AP Rabbit Poly ELISA, IHC, WB CD328SIGLEC713939-1-AP Rabbit Poly ELISA, FC, IHC, WBCD329SIGLEC913377-1-AP Rabbit Poly ELISA, IHC, WB CD32a CD3215625-1-AP Rabbit Poly ELISA, FC, WB CD33CD3317425-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD332FGFR213042-1-AP Rabbit Poly ELISA, IF, IHC, WBCD334FGFR411098-1-APRabbit PolyELISA, IF, IHC, WBCD No.Antibody NameCat. No.TypeApplicationsCD336CD33655118-1-AP Rabbit Poly ELISA, WB CD338BCRP/ABCG210051-1-AP Rabbit Poly ELISA, IHC, WB CD34CD3414486-1-AP Rabbit Poly ELISA, IF, IHC, WBCD340HER2/ErbB218299-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD340HER2/ErbB251105-1-AP Rabbit Poly ELISA, IF, IHC, WB CD349Frizzled 913865-1-AP Rabbit Poly ELISA, WB CD350Frizzled 1018175-1-AP Rabbit Poly ELISA, IHC, WB CD354TREM111791-1-APRabbit Poly ELISA, FC, IF, WB CD36CD3618836-1-APRabbit Poly ELISA, FC, IF, IHC, WB CD360IL21R 10533-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD363S1PR1/EDG155133-1-AP Rabbit Poly ELISA, IHC, IP , WBCD37CD3721044-1-AP Rabbit PolyELISA, IHC, WBCD38CD3860006-1-Ig MouseMono ELISA, IF, WBCD39CD3919229-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD3d CD3 delta 16669-1-AP Rabbit PolyELISA, FC, IHC, IP , WBCD3d CD3 delta 60194-1-Ig MouseMono ELISA, WBCD3e CD3 epsilon 17617-1-AP Rabbit PolyELISA, FC, IF, IHC, IP , WBCD3e CD3 epsilon 60181-1-Ig Mouse Mono ELISA, FC, IHC, WB CD3g CD3 gamma 21120-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD4CD411056-2-AP Rabbit Poly ELISA, IHC, IP , WBCD4CD419068-1-AP Rabbit Poly ELISA, IP , WB CD42a GP914564-1-AP Rabbit Poly ELISA, IHC, WB CD42b CD42b 12860-1-AP Rabbit Poly ELISA, IHC, WB CD43CD4313959-1-APRabbit Poly ELISA, IHC, WB CD44CD4415675-1-APRabbit PolyELISA, FC, IF, IHC, IP , WB1222211332312452267251118616CD31HER2/ErbB2CD38LEPR E-cadherin TREM1More validation images available on our website.CD No.Antibody NameCat. No.Type ApplicationsCD44CD4460224-1-IgMouse Mono ELISA, FC, IF, IHC, WB CD45CD4520103-1-APRabbit Poly ELISA, FC, IHC, IP , Polymer Detection Kit, WBCD46CD4612494-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD47CD4720305-1-APRabbit PolyELISA, IHC, WBCD49c Integrin alpha-366070-1-Ig MouseMono ELISA, FC, IF, WBCD49c Integrin alpha-321992-1-APRabbit Poly ELISA, FC, IF, IP , WB CD49d Integrin alpha-419676-1-AP Rabbit Poly ELISA, IHC, WB CD49e Integrin alpha-510569-1-AP Rabbit Poly ELISA, IF, WBCD49f Integrin alpha 6X1A-Specific 20231-1-APRabbit Poly ELISA, WBCD49f Integrin alpha 6X1B-Specific 20232-1-AP Rabbit Poly ELISA, WB CD5CD517227-1-AP Rabbit Poly ELISA, IHC, IP , WBCD50ICAM317699-1-APRabbit Poly ELISA, FC, IHC, WBCD51Integrin beta-318309-1-APRabbit Poly ELISA, IHC, WB CD54ICAM-110831-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD55ICAM-115364-1-AP Rabbit Poly ELISA, FC, IHC, IP , WB CD56NCAM1/CD5614255-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD56Icam-116174-1-AP Rabbit PolyELISA, IF, IHC, IP , WBCD56NCAM1/CD5660238-1-Ig MouseMono ELISA, IHC, WBCD58CD5810878-1-APRabbit Poly ELISA, WB CD58Icam110020-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD59CD5910742-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB CD61Integrin beta-318309-1-AP Rabbit Poly ELISA, IHC, WBCD62E E-selectin/CD62E 20894-1-AP Rabbit Poly ELISA, FC, WBCD62P P-selectin 13304-1-AP Rabbit Poly ELISA, IHCCD66d CEACAM3-Specific 19496-1-APRabbit Poly ELISA, FC, IHCCD66e CEA 10421-1-AP Rabbit Poly ELISA, IHC CD66f PSG111809-1-AP Rabbit Poly ELISA, IF, IHC, WBCD69CD6910803-1-AP Rabbit Poly ELISA, IHC, WB CD7CD711320-1-AP Rabbit PolyELISA, IHCCD7CD760209-2-IgMouseMono ELISA, IHC, WBCD71Transferrin receptor/CD7110084-2-APRabbit PolyELISA, IF, IHC, IP , WBCD No.Antibody NameCat. No.TypeApplicationsCD72CD7213469-1-AP Rabbit Poly ELISA, WB CD73NT5E/CD7312231-1-AP Rabbit Poly ELISA, FC, IHC, WBCD75ST6GAL114355-1-AP Rabbit Poly ELISA, IHC, IP , WBCD79a CD79A 22349-1-AP Rabbit Poly ELISA, IHC, WB CD80CD8014292-1-AP Rabbit Poly ELISA, FC, WB CD81CD8118250-1-AP Rabbit Poly ELISA, FC, IHC CD82CD8210248-1-AP Rabbit Poly ELISA, IHC, WB CD84CD8412566-1-AP Rabbit Poly ELISA, WB CD85CLILRB512881-1-AP Rabbit Poly ELISA, WB CD85FLILRA518821-1-AP Rabbit Poly ELISA, FC, WB CD85G LILRA421153-1-AP Rabbit Poly ELISA, WB CD85H LILRA211461-1-AP Rabbit Poly ELISA, IHC, WB CD86CD8613395-1-AP Rabbit Poly ELISA, FC, IHC, WBCD87uPAR 10286-1-AP Rabbit Poly ELISA, FC, IF;FC, IP , WB CD88C5AR121316-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD89CD8913245-1-AP Rabbit Poly ELISA, WB CD8b CD8B 21256-1-AP Rabbit Poly ELISA, WB CD9CD920597-1-AP Rabbit PolyELISA, FC, IHC, WBCD9CD960232-1-Ig MouseMono ELISA, IHC, WBCD94CD9413332-1-APRabbit Poly ELISA, IHC, WB CD95FAS/CD9513098-1-APRabbit PolyELISA, FC, IF, IHC, IP , WBCD95FAS/CD9560196-1-Ig Mouse Mono ELISA, FC, IHC, WB CD97CD9713071-1-AP Rabbit Poly ELISA, FC, IHC, WBCD98CD9815193-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP , WB CD99CD9923079-1-AP Rabbit Poly ELISA, FC, IHC, WB CDw92SLC44A114687-1-APRabbit PolyELISA, WB1111111321175221173115431322111 THE BENCHMARKIN VALIDATIONProteintech take complete responsibility for every product theymanufacture and ensure that it will work in your hands with anextensive validation process.Here’s what you can expect:• Every antibody has been extensively validated by the in-houseteam in a wide range of applications.• Antibodies now tested in siRNA knockdown todemonstrate specificity.• Original validation data available to researcher on request.• Multi-lingual technical support available online and by email.Please Note: All products featured in this product focus are for research use only.LIVE CHAT TWITTER@proteintechBLOG /news/blogYOUTUBE /ProteintechSupport Available 24 hours via Live Chat and 9-5 (CDT) via phone. Email support also available. 1 (888) 4PTGLAB(1-888-478-4522) (toll free in USA),or 1(312) 455-8498 (outside USA)1 (312) 455-8408Proteintech Group, Inc. 5400 Pearl Street, Suite 300, Rosemont, IL 60018, USA**********************PHONE FAX ADDRESS EMAIL PHONE EMAIL FAX ADDRESS EMAIL +44 (161) 8393007+44 (161) 2413103Proteintech Europe, Ltd. 4th Floor, 196 Deansgate, Manchester, M3 3WF***********************************Sales and technical support only.PHONE FAX ADDRESS EMAIL ************or 4006-900-926************Wuhan Sanying Biotechnologies D3-3, No.666 Gaoxin Avenue, Wuhan East Lake Hi-tech Development Zone Wuhan, Hubei, P .R.C******************We are ISO 9001 and ISO 13485 accredited.Proteintech Group US Head Office Proteintech Europe United Kingdom Proteintech Europe Germany Proteintech China Office CONTACT US。

产品名称Anti-Nuclear Pore Complex 蛋白抗体[Mab414]描述小鼠单克隆抗体[Mab414] to 核Pore Complex 蛋白宿主Mouse 经测试应用适用于: ICC/IF 种属反应性与反应: Mouse, Human, Saccharomyces cerevisiae预测可用于: Vertebrata免疫原Full length native protein (purified) corresponding to Human Nuclear Pore Complex Proteins.Nuclear Pore Complex Proteins阳性对照Raw, HEK 293 cell lysate (see Abreview), rat liver lysate (see Aris reference)常规说明This is a reliable general purpose monoclonal antibody which recognizes a related family of NPCproteins. This antibody is ideal for studying the morphology and composition of the nucleus andnuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis andmeiosis.This product was changed from ascites to tissue culture supernatant on 20/05/2019. Please notethat the dilutions may need to be adjusted accordingly. If you have any questions, please do nothesitate to contact our scientific support team.The Life Science industry has been in the grips of a reproducibility crisis for a number of years.Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodiesand knockout edited cell lines for gold-standard validation. Please check that this product meetsyour needs before purchasing.If you have any questions, special requirements or concerns, please send us an inquiry and/orcontact our Support team ahead of purchase. Recommended alternatives for this product can befound below, along with publications, customer reviews and Q&As 形式Liquid 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.存储溶液Preservative: 0.03% Thimerosal (merthiolate)Constituent: PBSProduct datasheetAnti-Nuclear Pore Complex Proteins antibody [Mab414] ab2460911 Abreviews 161 References 4 图像概述性能纯度Affinity purified Primary antibody 说明This is a reliable general purpose monoclonal antibody which recognizes a related family of NPCproteins. This antibody is ideal for studying the morphology and composition of the nucleus andnuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis andmeiosis.克隆单克隆克隆编号Mab414同种型IgG1The Abpromise guarantee Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Pore Complex Proteins antibody [Mab414](ab24609)Image courtesy of Saram N J et al. J Biol Chem. 2011Nov 11; 286(45): 38989–39001. doi:10.1074/jbc.M 111.297952ab24609 staining Nuclear Pore Complex Proteins in Humanchronic myelogenous leukemia cells from bone marrow cells. Cellswere fixed with 4% paraformaldehyde in DPBS buffer for 20mins and permeabilised with 0.1% Triton X-100 for 20 min at roomtemperature. Cells were blocked with 2% normal goat serum inDPBS with 1% BSA and washed with 0.1% Tween 20.This image was generated using the ascites version of the product.应用Abpromise™承诺保证使用ab24609于以下的经测试应用“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。