Comparison the neuropreotective effect of Cortex Phellodendri chinensis
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Introduction to Artificial Intelligence智慧树知到课后章节答案2023年下哈尔滨工程大学哈尔滨工程大学第一章测试1.All life has intelligence The following statements about intelligence arewrong()A:All life has intelligence B:Bacteria do not have intelligence C:At present,human intelligence is the highest level of nature D:From the perspective of life, intelligence is the basic ability of life to adapt to the natural world答案:Bacteria do not have intelligence2.Which of the following techniques is unsupervised learning in artificialintelligence?()A:Neural network B:Support vector machine C:Decision tree D:Clustering答案:Clustering3.To which period can the history of the development of artificial intelligencebe traced back?()A:1970s B:Late 19th century C:Early 21st century D:1950s答案:Late 19th century4.Which of the following fields does not belong to the scope of artificialintelligence application?()A:Aviation B:Medical C:Agriculture D:Finance答案:Aviation5.The first artificial neuron model in human history was the MP model,proposed by Hebb.()A:对 B:错答案:错6.Big data will bring considerable value in government public services, medicalservices, retail, manufacturing, and personal location services. ()A:错 B:对答案:对第二章测试1.Which of the following options is not human reason:()A:Value rationality B:Intellectual rationality C:Methodological rationalityD:Cognitive rationality答案:Intellectual rationality2.When did life begin? ()A:Between 10 billion and 4.5 billion years B:Between 13.8 billion years and10 billion years C:Between 4.5 billion and 3.5 billion years D:Before 13.8billion years答案:Between 4.5 billion and 3.5 billion years3.Which of the following statements is true regarding the philosophicalthinking about artificial intelligence?()A:Philosophical thinking has hindered the progress of artificial intelligence.B:Philosophical thinking has contributed to the development of artificialintelligence. C:Philosophical thinking is only concerned with the ethicalimplications of artificial intelligence. D:Philosophical thinking has no impact on the development of artificial intelligence.答案:Philosophical thinking has contributed to the development ofartificial intelligence.4.What is the rational nature of artificial intelligence?()A:The ability to communicate effectively with humans. B:The ability to feel emotions and express creativity. C:The ability to reason and make logicaldeductions. D:The ability to learn from experience and adapt to newsituations.答案:The ability to reason and make logical deductions.5.Which of the following statements is true regarding the rational nature ofartificial intelligence?()A:The rational nature of artificial intelligence includes emotional intelligence.B:The rational nature of artificial intelligence is limited to logical reasoning.C:The rational nature of artificial intelligence is not important for itsdevelopment. D:The rational nature of artificial intelligence is only concerned with mathematical calculations.答案:The rational nature of artificial intelligence is limited to logicalreasoning.6.Connectionism believes that the basic element of human thinking is symbol,not neuron; Human's cognitive process is a self-organization process ofsymbol operation rather than weight. ()A:对 B:错答案:错第三章测试1.The brain of all organisms can be divided into three primitive parts:forebrain, midbrain and hindbrain. Specifically, the human brain is composed of brainstem, cerebellum and brain (forebrain). ()A:错 B:对答案:对2.The neural connections in the brain are chaotic. ()A:对 B:错答案:错3.The following statement about the left and right half of the brain and itsfunction is wrong ().A:When dictating questions, the left brain is responsible for logical thinking,and the right brain is responsible for language description. B:The left brain is like a scientist, good at abstract thinking and complex calculation, but lacking rich emotion. C:The right brain is like an artist, creative in music, art andother artistic activities, and rich in emotion D:The left and right hemispheres of the brain have the same shape, but their functions are quite different. They are generally called the left brain and the right brain respectively.答案:When dictating questions, the left brain is responsible for logicalthinking, and the right brain is responsible for language description.4.What is the basic unit of the nervous system?()A:Neuron B:Gene C:Atom D:Molecule答案:Neuron5.What is the role of the prefrontal cortex in cognitive functions?()A:It is responsible for sensory processing. B:It is involved in emotionalprocessing. C:It is responsible for higher-level cognitive functions. D:It isinvolved in motor control.答案:It is responsible for higher-level cognitive functions.6.What is the definition of intelligence?()A:The ability to communicate effectively. B:The ability to perform physicaltasks. C:The ability to acquire and apply knowledge and skills. D:The abilityto regulate emotions.答案:The ability to acquire and apply knowledge and skills.第四章测试1.The forward propagation neural network is based on the mathematicalmodel of neurons and is composed of neurons connected together by specific connection methods. Different artificial neural networks generally havedifferent structures, but the basis is still the mathematical model of neurons.()A:对 B:错答案:对2.In the perceptron, the weights are adjusted by learning so that the networkcan get the desired output for any input. ()A:对 B:错答案:对3.Convolution neural network is a feedforward neural network, which hasmany advantages and has excellent performance for large image processing.Among the following options, the advantage of convolution neural network is().A:Implicit learning avoids explicit feature extraction B:Weight sharingC:Translation invariance D:Strong robustness答案:Implicit learning avoids explicit feature extraction;Weightsharing;Strong robustness4.In a feedforward neural network, information travels in which direction?()A:Forward B:Both A and B C:None of the above D:Backward答案:Forward5.What is the main feature of a convolutional neural network?()A:They are used for speech recognition. B:They are used for natural languageprocessing. C:They are used for reinforcement learning. D:They are used forimage recognition.答案:They are used for image recognition.6.Which of the following is a characteristic of deep neural networks?()A:They require less training data than shallow neural networks. B:They havefewer hidden layers than shallow neural networks. C:They have loweraccuracy than shallow neural networks. D:They are more computationallyexpensive than shallow neural networks.答案:They are more computationally expensive than shallow neuralnetworks.第五章测试1.Machine learning refers to how the computer simulates or realizes humanlearning behavior to obtain new knowledge or skills, and reorganizes the existing knowledge structure to continuously improve its own performance.()A:对 B:错答案:对2.The best decision sequence of Markov decision process is solved by Bellmanequation, and the value of each state is determined not only by the current state but also by the later state.()A:对 B:错答案:对3.Alex Net's contributions to this work include: ().A:Use GPUNVIDIAGTX580 to reduce the training time B:Use the modified linear unit (Re LU) as the nonlinear activation function C:Cover the larger pool to avoid the average effect of average pool D:Use the Dropouttechnology to selectively ignore the single neuron during training to avoid over-fitting the model答案:Use GPUNVIDIAGTX580 to reduce the training time;Use themodified linear unit (Re LU) as the nonlinear activation function;Cover the larger pool to avoid the average effect of average pool;Use theDropout technology to selectively ignore the single neuron duringtraining to avoid over-fitting the model4.In supervised learning, what is the role of the labeled data?()A:To evaluate the model B:To train the model C:None of the above D:To test the model答案:To train the model5.In reinforcement learning, what is the goal of the agent?()A:To identify patterns in input data B:To minimize the error between thepredicted and actual output C:To maximize the reward obtained from theenvironment D:To classify input data into different categories答案:To maximize the reward obtained from the environment6.Which of the following is a characteristic of transfer learning?()A:It can only be used for supervised learning tasks B:It requires a largeamount of labeled data C:It involves transferring knowledge from onedomain to another D:It is only applicable to small-scale problems答案:It involves transferring knowledge from one domain to another第六章测试1.Image segmentation is the technology and process of dividing an image intoseveral specific regions with unique properties and proposing objects ofinterest. In the following statement about image segmentation algorithm, the error is ().A:Region growth method is to complete the segmentation by calculating the mean vector of the offset. B:Watershed algorithm, MeanShift segmentation,region growth and Ostu threshold segmentation can complete imagesegmentation. C:Watershed algorithm is often used to segment the objectsconnected in the image. D:Otsu threshold segmentation, also known as themaximum between-class difference method, realizes the automatic selection of global threshold T by counting the histogram characteristics of the entire image答案:Region growth method is to complete the segmentation bycalculating the mean vector of the offset.2.Camera calibration is a key step when using machine vision to measureobjects. Its calibration accuracy will directly affect the measurementaccuracy. Among them, camera calibration generally involves the mutualconversion of object point coordinates in several coordinate systems. So,what coordinate systems do you mean by "several coordinate systems" here?()A:Image coordinate system B:Image plane coordinate system C:Cameracoordinate system D:World coordinate system答案:Image coordinate system;Image plane coordinate system;Camera coordinate system;World coordinate systemmonly used digital image filtering methods:().A:bilateral filtering B:median filter C:mean filtering D:Gaussian filter答案:bilateral filtering;median filter;mean filtering;Gaussian filter4.Application areas of digital image processing include:()A:Industrial inspection B:Biomedical Science C:Scenario simulation D:remote sensing答案:Industrial inspection;Biomedical Science5.Image segmentation is the technology and process of dividing an image intoseveral specific regions with unique properties and proposing objects ofinterest. In the following statement about image segmentation algorithm, the error is ( ).A:Otsu threshold segmentation, also known as the maximum between-class difference method, realizes the automatic selection of global threshold T by counting the histogram characteristics of the entire imageB: Watershed algorithm is often used to segment the objects connected in the image. C:Region growth method is to complete the segmentation bycalculating the mean vector of the offset. D:Watershed algorithm, MeanShift segmentation, region growth and Ostu threshold segmentation can complete image segmentation.答案:Region growth method is to complete the segmentation bycalculating the mean vector of the offset.第七章测试1.Blind search can be applied to many different search problems, but it has notbeen widely used due to its low efficiency.()A:错 B:对答案:对2.Which of the following search methods uses a FIFO queue ().A:width-first search B:random search C:depth-first search D:generation-test method答案:width-first search3.What causes the complexity of the semantic network ().A:There is no recognized formal representation system B:The quantifiernetwork is inadequate C:The means of knowledge representation are diverse D:The relationship between nodes can be linear, nonlinear, or even recursive 答案:The means of knowledge representation are diverse;Therelationship between nodes can be linear, nonlinear, or even recursive4.In the knowledge graph taking Leonardo da Vinci as an example, the entity ofthe character represents a node, and the relationship between the artist and the character represents an edge. Search is the process of finding the actionsequence of an intelligent system.()A:对 B:错答案:对5.Which of the following statements about common methods of path search iswrong()A:When using the artificial potential field method, when there are someobstacles in any distance around the target point, it is easy to cause the path to be unreachable B:The A* algorithm occupies too much memory during the search, the search efficiency is reduced, and the optimal result cannot beguaranteed C:The artificial potential field method can quickly search for acollision-free path with strong flexibility D:A* algorithm can solve theshortest path of state space search答案:When using the artificial potential field method, when there aresome obstacles in any distance around the target point, it is easy tocause the path to be unreachable第八章测试1.The language, spoken language, written language, sign language and Pythonlanguage of human communication are all natural languages.()A:对 B:错答案:错2.The following statement about machine translation is wrong ().A:The analysis stage of machine translation is mainly lexical analysis andpragmatic analysis B:The essence of machine translation is the discovery and application of bilingual translation laws. C:The four stages of machinetranslation are retrieval, analysis, conversion and generation. D:At present,natural language machine translation generally takes sentences as thetranslation unit.答案:The analysis stage of machine translation is mainly lexical analysis and pragmatic analysis3.Which of the following fields does machine translation belong to? ()A:Expert system B:Machine learning C:Human sensory simulation D:Natural language system答案:Natural language system4.The following statements about language are wrong: ()。
阴极经颅直流电刺激治疗急性期脑卒中的效果及对神经功能的影响宋大伟① 于红日② 【摘要】 目的:探究阴极经颅直流电刺激治疗急性期脑卒中的效果,及对神经功能的影响。
方法:在2019年9月—2021年9月展开此次研究,选取此期间在乳山市人民医院接受治疗的急性期脑卒中患者82例,应用随机数表法将其分为对照组(n=41)及观察组(n=41)。
对照组接受常规康复训练,观察组在对照组基础上加用阴极经颅直流电刺激治疗。
对比两组运动功能[Fugl-Meyer评估表(FMA)、上肢动作研究量表(ARAT)]、日常生活能力[改良Barthel指数(MBI)]、神经功能缺损情况及认知功能[神经功能缺损程度评分(NDS)、欧洲脑卒中量表(ESS)、蒙特利尔认知评估量表(MoCA)]、炎症因子[超敏C反应蛋白(hs-CRP)、超氧化物歧化酶(SOD)、白介素-10(IL-10)、丝氨酸蛋白酶抑制物]、神经因子[缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)、脑源性神经营养因子(BNDF)]。
结果:治疗前,两组FMA、ARAT、MBI评分比较,差异均无统计学意义(P>0.05);治疗后,两组FMA、ARAT、MBI评分均升高,且观察组均高于对照组(P<0.05)。
治疗前,两组NDS、ESS、MoCA评分比较,差异均无统计学意义(P>0.05);治疗后,两组NDS评分均降低,观察组低于对照组,两组MoCA、ESS评分均升高,观察组均高于对照组(P<0.05)。
治疗前,两组hs-CRP、SOD、IL-10、丝氨酸蛋白酶抑制剂水平比较,差异均无统计学意义(P>0.05);治疗后,两组hs-CRP、IL-10水平均降低,观察组均低于对照组,两组SOD、丝氨酸蛋白酶抑制剂水平均升高,观察组均高于对照组(P<0.05)。
治疗前,两组HIF-1α、VEGF、BNDF水平比较,差异均无统计学意义(P>0.05);治疗后,两组HIF-1α水平均降低,观察组低于对照组,两组VEGF、BNDF水平均升高,观察组均高于对照组(P<0.05)。
2024年6月大学英语六级考试真题和答案(第1套)Part I Writing (30 minutes)Directions: For this part, you are allowed 30 minutes to write an essay that begins with the sentence “There is a growing awareness of the importance of digital literacy and skills in today’s world.” You can make comments, cite examples or use your personal experiences to develop your essay. You should write at least 150 words but no more than 200 words. You should copy the sentence given in quotes at the beginning of your essay.Part Ⅱ Listening Comprehension (30 minutes)Section ADirections:In this section, you will hear two long conversations. At the end of each conversation, you will hear four questions. Both the conversation and the questions will be spoken only once. After you hear a question, you must choose the best answer from the four choices marked A), B), C) and D). Then mark the corresponding letter on Answer Sheet 1 with a single line through the centre.Questions 1 to 4 are based on the conversation you have just heard.1. A) Reply to the man’s last proposal within a short time.B) Sign the agreement if one small change is made to it.C) Make a sponsorship deal for her client at the meeting.D) Give the man some good news regarding the contract.2. A) They are becoming impatient.B) They are afraid time is running out.C) They are used to making alterations.D) They are concerned about the details.3. A) To prevent geographical discrimination.B) To tap the food and beverage market.C) To avoid any conflict of interest.D) To reduce unfair competition.4. A) It is a potential market for food and beverage.B) It is very attractive for real estate developers.C) It is a negligible market for his company.D) It is very different from other markets.Questions 5 to 8 are based on the conversation you have just heard.5. A) They are thrilled by a rare astronomic phenomenon.B) They are celebrating a big event on mountain tops.C) They are enthusiastic about big science-related stories.D) They are joined by astronomers all across North America.6. A) It will be the most formidable of its kind in over a century.B) It will come closest to Earth in more than one hundred years.C) It will eclipse many other such events in human history.D) It will be seen most clearly from Denver’s mountain tops.7. A) A blur.B) Stars.C) The edge of our galaxy.D) An ordinary flying object.8. A) Use professional equipment.B) Climb to the nearby heights.C) Fix their eyes due north.D) Make use of phone apps.Section BDirections: In this section, you will hear two passages. At the end of each passage, you will hear three or four questions. Both the passage and the questions will be spoken only once. After you hear a question, you must choose the best answer from the four choices marked A),B),C) and D). Then mark the corresponding letter on Answer Sheet 1 with a single line through the centre.Questions 9 to 11 are based on the passage you have just heard.9. A) Whether consumers should be warned against ultra-processed foods.B) Whether there is sufficient scientific consensus on dietary guidelines.C) Whether guidelines can form the basis for nutrition advice to consumers.D) Whether food scientists will agree on the concept of ultra-processed foods.10. A) By the labor cost for the final products.B) By the degree of industrial processing.C) By the extent of chemical alteration.D) By the convention of classification.11. A) Increased consumers’ expenses.B) Greater risk of chronic diseases.C) People’s misunderstanding of nutrition.D) Children’s dislike for unprocessed foods.Questions 12 to 15 are based on the passage you have just heard.12. A) They begin to think of the benefits of constraints.B) They try to seek solutions from creative people.C) They try hard to maximize their mental energy.D) They begin to see the world in a different way.13. A) It is characteristic of all creative people.B) It is essential to pushing society forward.C) It is a creative person’s response to limitation.D) It is an impetus to socio-economic development.14. A) Scarcity or abundance of resources has little impact on people’s creativity.B) Innovative people are not constrained in connecting unrelated concepts.C) People have no incentive to use available resources in new ways.D) Creative people tend to consume more available resources.15. A) It is key to a company’s survival.B) It shapes and focuses problems.C) It is essential to meeting challenges.D) It thrives best when constrained.Section CDirections: In this section, you will hear three recordings of lectures or talks followed by three or four questions. The recordings will be played only once. After you hear a question, you must choose the best answer from the four choices marked A), B), C) and D). Then mark the corresponding letter on Answer Sheet 1 with a single line through the centre.Questions 16 to 18 are based on the recording you have just heard.16. A) Because they are learned.B) Because they come naturally.C) Because they have to be properly personalized.D) Because there can be more effective strategies.17. A) The extent of difference and of similarity between the two sides.B) The knowledge of the specific expectation the other side holds.C) The importance of one’s goals and of the relationship.D) The approaches one adopts to conflict management.18. A) The fox.B) The owl.C) The shark.D) The turtle.Questions 19 to 21 are based on the recording you have just heard.19. A) Help save species from extinction and boost human health.B) Understand how plants and animals perished over the past.C) Help gather information publicly available to researchers.D) Find out the cause of extinction of Britain’s 66,000 species.20. A) It was once dominated by dinosaurs.B) It has entered the sixth mass extinction.C) Its prospects depend on future human behaviour.D) Its climate change is aggravated by humans.21. A) It dwarfs all other efforts to conserve, protect and restorebiodiversity on earth.B) It is costly to get started and requires the joint efforts of thousands of scientists.C) It can help to bring back the large numbers of plants and animals that have gone extinct.D) It is the most exciting, most relevant, most timely and most internationally inspirational.Questions 22 to 25 are based on the recording you have just heard.22. A) Cultural identity.B) Social evolution.C) The Copernican revolution.D) Human individuality.23. A) It is a delusion to be disposed of.B) It is prevalent even among academics.C) It is a myth spread by John Donne’s poem.D) It is rooted in the mindset of the 17th century.24. A) He believes in Copernican philosophical doctrines about the universe.B) He has gained ample scientific evidence at the University of Reading.C) He has found that our inner self and material self are interconnected.D) He contends most of our body cells can only live a few days or weeks.25. A) By coming to see how disruptive such problems have got to be.B) By realising that we all can do our own bit in such endeavours.C) By becoming aware that we are part of a bigger world.D) By making joint efforts resolutely and persistently.Part III Reading Comprehension (40 minutes)Section ADirections: In this section, there is a passage with ten blanks. You are required to select one word for each blank from a list of choices given in a word bank following the passage. Read the passage through carefully before making your choices. Each choice in the bank is identified by a letter. Please mark the corresponding letter for each item on Answer Sheet 2with a single line through the centre. You may not use any of the words in the bank more than once.It’s quite remarkable how different genres of music can spark unique feelings, emotions, and memories. Studies have shown that music can reduce stress and anxiety before surgeries and we are all attracted toward our own unique life soundtrack.If you’re looking to____26____stress, you might want to give classical music a try.The sounds of classical music produce a calming effectletting____27____pleasure-inducing dopamine (多巴胺) in the brain thathelps control attention, learning and emotional responses.It can also turn down the body’s stress response, resulting in an overall happier mood. It turns out a pleasant mood can lead to____28____in a person’s thinking.Although there are many great____29____of classical music like Bach, Beethoven and Handel, none of these artists’ music seems to have the same health effects as Mozart’s does. According to researchers, listening to Mozart can increase brain wave activity and improve____30____function. Another study found that the distinctive features of Mozart’s music trigger parts of the brain that are responsible for high-level mental functions. Even maternity____31____use Mozart to help newborn babies adapt to life outside of the mother’s belly.It has been found that listening to classical music____32____reduces a person’s blood pressure. Researchers believe that the calming sounds of classical music may help your heart____33____from stress. Classical music can also be a great tool to help people who have trouble sleeping. One study found that students who had trouble sleeping slept better while they were listening to classical music.Whether classical music is something that you listen to on a regular basis or not, it wouldn’t____34____to take time out of your day to listen to music that you find____35____. You will be surprised at how good it makes you feel and the potentially positive change in your health.A) alleviateB) clarityC) cognitiveD) composersE) hurtF) inhibitingG) interrogationH) intrinsicallyI) looseJ) majesticK) mandatoryL) recoverM) significantlyN) soothingO) wardsSection BDirections: In this section, you are going to read a passage with ten statements attached to it. Each statement contains information given in one of the paragraphs. Identify the paragraph from which the information is derived. You may choose a paragraph more than once. Each paragraph is marked with a letter. Answer the questions by marking the correspondingletter on Answer Sheet 2.The Curious Case of the Tree That Owns ItselfA) In the city of Athens, Georgia, there exists a rather curious local landmark—a large white oak that is almost universally stated to own itself. Because of this, it is considered one of the most famous trees in the world. So how did this tree come to own itself and the land around it?B) Sometime in the 19th century a Georgian called Colonel William Jackson reportedly took a liking to the said tree and endeavored to protect it from any danger. As to why he loved it so, the earliest documented account of this story is an anonymously written front page article in the Athens Weekly Banner published on August 12, 1890. It states, “Col. Jackson had watched the tree grow from his childhood, and grew to love it almost as he would a human. Its luxuriant leaves and sturdy limbs had often protected him from the heavy rains,and out of its highest branches he had many a time gotten the eggs of the feathered singers.He watched its growth, and when reaching a ripe old age he saw the tree standing in its magnificent proportions, he was pained to think that after his death it would fall into the hands of those who might destroy it.”C) Towards this end, Jackson transferred by means of a deed ownership of the tree and a little land around it to the tree itself. The deed read, “W. H. Jackson for and in consideration of the great affection which he bears the said tree, and his great desire to see it protected has conveyed unto the said oak tree entire possession of itself and of all land within eight feet of it on all sides.”D) In time, the tree came to be something of a tourist attraction, known as The Tree That Owns Itself. However, in the early 20th century, the tree started showing signs of its slow death,with little that could be done about it. Father time comes for us all eventually, even our often long lived, tall and leafy fellow custodians (看管者) of Earth. Finally, on October 9,1942, the over 30 meter tall and 200-400 year old tree fell, rumor has it, as a result of a severe windstorm and/or via having previously died and its roots rotted.E) About four years later, members of the Junior Ladies Garden Club (who’d tended to the tree before its unfortunate death) tracked down a small tree grown from a nut taken from the original tree. And so it was that on October 9, 1946, under the direction of Professor Roy Bowden of the College of Agriculture at the University of Georgia, this little tree was transplanted to the location of its ancestor. A couple of months later, an official ceremony was held featuring none other than the Mayor of Athens, Robert L McWhorter, to commemorate the occasion.F) This new tree became known as The Son of the Tree That Owns Itself and it was assumed that, as the original tree’s heir, it naturally inherited the land it stood on. Of course, there are many dozens of othertrees known to exist descending from the original, as people taking a nut from it to grow elsewhere was a certainty. Thatsaid, to date, none of the original tree’s other children have petitioned the courts for their share of the land, so it seems all good. In any event, The Son of the Tree That Owns Itself still stands today, though often referred to simply as The Tree That Owns Itself.G) This all brings us around to whether Jackson ever actually gave legal ownership of the tree to itself in the first place and whether such a deed is legally binding.H) Well, to begin with, it turns out Jackson only spent about three years of his life in Athens,starting at the age of 43 from 1829 to 1832, sort of dismissing the idea that he loved the tree from spending time under it as a child and watching it grow, and then worrying about what would happen to it after he died. Further, an extensive search of land ownership records in Athens does not seem to indicate Jackson ever owned the land the tree sits on.I) He did live on a lot of land directly next to it for those three years, but whether he owned that land or not isn’t clear. Whatever the case, in 1832 a four acre parcel, which included the land the tree was on and the neighboring land Jackson lived on, among others, was sold to University professor Malthus A Ward. In the transaction, Ward was required to pay Jackson a sum of $1,200 (about $31,000 today), either for the property itself or simply in compensation for improvements Jackson had made on the lot. In the end, whether he ever owned the neighboring lot or was simply allowed to use it while he allegedly worked at the University, he definitely never owned the lot the tree grew on, which is the most important bit for the topic at hand.J) After Professor Ward purchased the land, Jackson and his family purchased a 655 acre parcel a few miles away and moved there. Ten years later, in 1844, Jackson seemed to have come into financial difficulties and had his little plantation seized by the Clarke County Sheriff’s office and auctioned off to settle the mortgage. Thus, had he owned some land in Athens itself, including the land the tree sat on, presumably he would have sold it to raise funds or otherwise had it taken as well.K) And whatever the case there, Jackson would have known property taxes needed to be paid on the deeded land for the tree to be truly secure in its future. Yet no account or record indicates any trust or the like was set up to facilitate this.L) On top of all this, there is no hard evidence such a deed ever existed, despite the fact that deed records in Athens go back many decades before Jackson’s death in 1876 and that it was supposed to have existed in 1890 in the archives according to the original anonymous news reporter who claims to have seen it.M) As you might imagine from all of this, few give credit to this sideof the story. So how did all of this come about then?N) It is speculated to have been invented by the imagination of the said anonymous author at the Athens Weekly Banner in the aforementioned 1890 front page article titled “Deeded to Itself”, which by the way contained several elements that are much more easily proved to be false. As to why the author would do this, it’s speculated perhaps it was a 19th century version of a click-bait thought exercise on whether it would be legal for someone to deed such a non-conscious living thing to itself or not.O) Whatever the case, the next known instance of the Tree That Owns Itself being mentioned wasn’t until 1901 in the Centennial Edition of that same paper, the Athens Weekly Banner. This featured another account very clearly just copying the original article published about a decade before, only slightly reworded. The next account was in 1906, again in the Athens Weekly Banner, again very clearly copying the original account, only slightly reworded, the 19th century equivalent of re-posts when the audience has forgotten about the original.36. Jackson was said to have transferred his ownership of the oak tree to itself in order to protect it from being destroyed.37. No proof has been found from an extensive search that Jackson had ever owned the land where the oak tree grew.38. When it was raining heavily, Jackson often took shelter under a big tree that is said to own itself.39. There is no evidence that Jackson had made arrangements to pay property taxes for the land on which the oak tree sat.40. Professor Ward paid Jackson over one thousand dollars when purchasinga piece of land from him.41. It is said the tree that owned itself fell in a heavy windstorm.42. The story of the oak tree is suspected to have been invented as a thought exercise.43. Jackson’s little plantation was auctioned off to settle his debt in the mid-19th century.44. An official ceremony was held to celebrate the transplanting of a small tree to where its ancestor had stood.45. The story of the Tree That Owns Itself appeared in the local paper several times, with slight alterations in wording.Section CDirections:There are 2 passages in this section. Each passage is followed by some questions or unfinished statements. For each of them there are four choices marked A), B), C) and D). You should decide on the best choice and mark the corresponding letter on Answer Sheet 2 with a single line through the centre.Passage OneQuestions 46 to 50 are based on the following passage.It is irrefutable that employees know the difference between right and wrong. So why don’t more employees intervene when they see someone exhibiting at-risk behavior in the workplace?There are a number of factors that influence whether people intervene. First, they need to be able to see a risky situation beginning to unfold. Second, the company’s culture needs to make them feel safe to speak up. And third, they need to have the communication skills to say something effectively.This is not strictly a workplace problem; it’s a growing problem off the job too. Every day people witness things on the street and choose to stand idly by. This is known as the bystander effect—the more people who witness an event, the less likely anyone in that group is to help the victim. The psychology behind this is called diffusion of responsibility. Basically, the larger the crowd, the more people assume that someone else will take care of it—meaning no one effectively intervenes or acts in a moment of need.This crowd mentality is strong enough for people to evade their known responsibilities. But it’s not only frontline workers who don’t make safety interventions in the workplace. There are also instances where supervisors do not intervene either.When a group of employees sees unsafe behavior not being addressed at a leadership level it creates the precedent that this is how these situations should be addressed, thus defining the safety culture for everyone.Despite the fact that workers are encouraged to intervene when they observe unsafe operations, this happens less than half of the time. Fear is the ultimate factor in not intervening. There is a fear of penalty, a fear that they’ll have to do more work if they intervene. Unsuccessful attempts in the past are another strong contributing factor to why people don’t intervene—they tend to prefer to defer that action to someone else for all future situations.On many worksites, competent workers must be appointed. Part of their job is to intervene when workers perform a task without the proper equipment or if the conditions are unsafe. Competent workers are also required to stop work from continuing when there’s a danger.Supervisors also play a critical role. Even if a competent person isn’t required, supervisors need a broad set of skills to not only identify and alleviate workplace hazards but also build a safety climate within their team that supports intervening and open communication among them.Beyond competent workers and supervisors, it’s important to educate everyone within the organization that they are obliged to intervene ifthey witness a possible unsafe act, whether you’re a designated competent person, a supervisor or a frontline worker.46. What is one of the factors contributing to failure of intervention in face of risky behavior in the workplace?A) Slack supervision style.B) Unfavorable workplace culture.C) Unforeseeable risk.D) Blocked communication.47. What does the author mean by “diffusion of responsibility” (Line 4, Para. 3)?A) The more people are around, the more they need to worry about their personal safety.B) The more people who witness an event, the less likely anyone will venture to participate.C) The more people idling around on the street, the more likely they need taking care of.D) The more people are around, the less chance someone will step forward to intervene.48. What happens when unsafe behavior at the workplace is not addressed by the leaders?A) No one will intervene when they see similar behaviors.B) Everyone will see it as the easiest way to deal with crisis.C) Workers have to take extra caution executing their duties.D) Workers are left to take care of the emergency themselves.49. What is the ultimate reason workers won’t act when they see unsafe operations?A) Preference of deferring the action to others.B) Anticipation of leadership intervention.C) Fear of being isolated by coworkers.D) Fear of having to do more work.50. What is critical to ensuring workplace safety?A) Workers be trained to operate their equipment properly.B) Workers exhibiting at-risk behavior be strictly disciplined.C) Supervisors create a safety environment for timely intervention.D) Supervisors conduct effective communication with frontline workers.Passage TwoQuestions 51 to 55 are based on the following passage.The term “environmentalist” can mean different things. It used to refer to people trying to protect wildlife and natural ecosystems. In the 21st century, the term has evolved to capture the need to combat human-made climate change.The distinction between these two strands of environmentalism is thecause of a split within the scientific community about nuclear energy.On one side are purists who believe nuclear power isn’t worth the risk and the exclusive solution to the climate crisis is renewable energy. The opposing side agrees that renewables are crucial, but says society needs an amount of power available to meet consumers’ basic demands when the sun isn’t shining and the wind isn’t blowing. Nuclear energy, being far cleaner than oil, gas and coal, is a natural option, especially where hydroelectric capacity is limited.Leon Clarke, who helped author reports for the UN’s Intergovernmental Panel on Climate Change, isn’t an uncritical supporter of nuclear energy, but says it’s a valuable option to have if we’re serious about reaching carbon neutrality.“Core to all of this is the degree to which you think we can actually meet climate goals with 100% renewables,” he said. “If you don’t believe we can do it, and you care about the climate, you are forced to think about something like nuclear.”The achievability of universal 100% renewability is similarly contentious. Cities such as Burlington, Vermont, have been “100% renewable” for years. But these cities often have small populations, occasionally still rely on fossil fuel energy and have significant renewable resources at their immediate disposal. Meanwhile, countries that manage to run off renewables typically do so thanks to extraordinary hydroelectric capabilities.Germany stands as the best case study for a large, industrialized country pushing into green energy. Chancellor Angela Merkel in 2011 announced Energiewende, an energy transition that would phase out nuclear and coal while phasing in renewables. Wind and solar power generation has increased over 400% since 2010, and renewables provided 46% of the country’s electricity in 2019.But progress has halted in recent years. The instability of renewables doesn’t just mean energy is often not produced at night, but also that solar and wind can overwhelm the grid during the day, forcing utilities to pay customers to use their electricity. Lagging grid infrastructure struggles to transport this overabundance of green energy from Germany’s north to its industrial south, meaning many factories still run on coal and gas. The political limit has also been reached in some places, with citizens meeting the construction of new wind turbines with loud protests.The result is that Germany’s greenhouse gas emissions have fallen by around 11.5% since 2010—slower than the EU average of 13.5%.51. What accounts for the divide within the scientific community about nuclear energy?A) Attention to combating human-made climate change.B) Emphasis on protecting wildlife and natural ecosystems.C) Evolution of the term ‘green energy’ over the last century.D) Adherence to different interpretations of environmentalism.52. What is the solution to energy shortage proposed by purists’opponents?A) Relying on renewables firmly and exclusively.B) Using fossil fuel and green energy alternately.C) Opting for nuclear energy when necessary.D) Limiting people’s non-basic consumption.53. What point does the author want to make with cities like Burlington as an example?A) It is controversial whether the goal of the whole world’s exclusive dependence on renewables is attainable.B) It is contentious whether cities with large populations have renewable resources at their immediate disposal.C) It is arguable whether cities that manage to run off renewables have sustainable hydroelectric capabilities.D) It is debatable whether traditional fossil fuel energy can be done away with entirely throughout the world.54. What do we learn about Germany regarding renewable energy?A) It has increased its wind and solar power generation four times over the last two decades.B) It represents a good example of a major industrialized country promoting green energy.C) It relies on renewable energy to generate more than half of its electricity.D) It has succeeded in reaching the goal of energy transition set by Merkel.55. What may be one of the reasons for Germany’s progress having halted in recent years?A) Its grid infrastructure’s capacity has fallen behind its development of green energy.B) Its overabundance of green energy has forced power plants to suspend operation during daytime.C) Its industrial south is used to running factories on conventional energy supplies.D) Its renewable energy supplies are unstable both at night and during the day.Part IV Translation (30 minutes)Directions: For this part, you are allowed 30 minutes to translate a passage from Chinese into English. You should write your answer on Answer Sheet 2.中国的传统婚礼习俗历史悠久,从周朝开始就逐渐形成了一套完整的婚礼仪式,有些一直沿用至今。
考研英语二阅读讲义篇章结构题(例证题&文章续写题)【例证题】通常情况下,例证题考查例子在文章中的作用。
根据例子在文章中所处的不同位置,将例证题进一步划分为1.开头举例2.细节例证3.全文例证【例证题-开头举例】1.例子的标志:(1)example,case,story(2)人名,数字,话语(直接引语&间接引语)2.题干特征:提问写作意图…is mentioned/noted to suggest that___.The example of…is used to show___.3.解题总则:开头举例目的是为了引出主题。
4.解题技巧开头举例型文章的主题在第一段结尾或第二段开头【典型例题】【2003-1】英一例:42.Donovan's story is mentioned in the text to_.[A]introduce the topic of online spying[B]show how he fought for the US[C]give an episode of the information war[D]honor his unique services to the CIA原文:Wild Bill Donovan would have loved the Internet.The American spymaster who built the Office of Strategic Services in the World WarⅡand later laid the roots for the CIA was fascinated with information....These days the Net,...,is reshaping Donovan‘s vocation as well.【典型例题】【2006-3】英一例:31.The extinction of large prehistoric animals is noted to suggest that_____[A]large animals were vulnerable to the changing environment[B]small species survived as large animals disappeared[C]large sea animals may face the same threat today[D]slow-growing fish outlive fast-growing ones原文:When prehistoric man arrived in new parts of the world,something strange happened to the large animals:they suddenly became extinct.Smaller species survived.The large, slow-growing animals were easy game,and were quickly hunted to extinction.Now something similar could be happening in the oceans.例题一【2010-2】例:29.Which of the following can best summarize the main idea of this text?[A]The moral decaying deserves more research by sociologists.[B]Marriage break-up stems from sex inequalities.[C]Husband and wife have different expectations from their marriage.[D]Conversational patterns between man and wife are different.原文Ⅰ①I was addressing a small gathering in a suburban Virginia living room—a women’s group that had invited men to join them.②Throughout the evening,one man had been particularly talkative,frequently offering ideas and anecdotes,while his wife sat silently beside him on the couch.③Toward the end of the evening,I commented that women frequently complain that their husbands don’t talk to them.④This man quickly nodded in agreement.⑤He gestured toward his wife and said,“She’s the talker in our family.”⑥The room burst into laughter;the man looked puzzled and hurt.⑦“It’s true,”he explained.⑧“When I come home from work I have nothing to say.⑨If she didn’t keep the conversation going,we’d spend the whole evening in silence.”Ⅱ①This episode crystallizes the irony that although American men tend to talk more than women in public situations,they often talk less at home.②And this pattern is wreaking havoc with marriage.例题二【2013-1】例:21.The joke in Paragraph1is used to illustrate_____.[A]the impact of technological advances[B]the alleviation of job pressure[C]the shrinkage of textile mills[D]the decline of middle-class incomes原文Ⅰ①In an essay entitled“Making It in America,”the author Adam Davidson relates a joke from cotton country about just how much a modern textile mill has been automated:The average mill has only two employees today,“a man and a dog.②The man is there to feed the dog,and the dog is there to keep the man away from the machines.”Ⅱ①Davidson’s article is one of a number of pieces that have recently appeared making the point that the reason we have such stubbornly high unemployment and declining middle-class incomes today is largely because of the big drop in demand because of the Great Recession,but it is also because of the advances in both globalization and the information technology revolution, which are more rapidly than ever replacing labor with machines or foreign workers.【例证题-细节例证】1.细节例证(小例证)的形式特征:段落中间(至少不在首句)2.解题技巧:返回原文,找到该例证所在位置,搜索该例证周围区域,找到例证支持的观点,通常是该段落首句或小例子的前一句。
2023年12月CET6大学英语六级考试真题及答案解析【官方完整版】Part I 写作Writing (30 minutes)Directions: Directions: For this part, you are allowed 30 minutes to write a short essay on innovation. Your essay should include the importance of innovation and measures to be taken to encourage innovation.You are required to write at least 150 words but no more than 200 words.【参照范文】It is universally acknowledged that innovation refers to being creative, unique and different. In fact, today it is impossibly difficult for us to image a 21st century without innovation.We should place a high value on innovation firstly because innovative spirit can enable an individual to ameliorate himself, so he can be equipped with capacity to see what others cannot see, be qualified for future career promotion, and be ready for meeting the forthcoming challenges. What’s more, we ought to attach importance to the role played by innovation in economic advancement. Put it another way, in this ever-changing world, innovation to economic growth is what water is to fish. To sum up, if innovation misses our attention in any possible way, we will suffer a great loss beyond imagination.In order to encourage innovation, it is wise for us to take some feasible measures. For example, mass media should greatly publicize the significance of creative spirit and encouragethe public to cultivate awareness of innovation. Besides, those who manage to innovate should be awarded generous prize. Though there is a long way ahead to go, I am firmly certain that the shared efforts will be paid off.【参照译文】众所周知创新意味着有发明力,独一无二和不一样。
医学文献检索作业专业:天然药物化学学号:*********姓名:***(一) 循环查找法课题:黄柏化学成分的研究主题词:黄柏(Phellodendri)、化学成分(chemical constituent)检索工具:维普数据库sciencedirect数据库1.维普数据库以黄柏和化学成分为限定主题词,限定年份在2009年—2013年,查找与课题有关的文献,检索结果为17篇。
如下图所示:可得到全文的文献有11篇,如下:(1)时东方, 张红晶, 郑梅竹, 等. 高速逆流色谱法分离白鲜皮中化学成分条件的优化[J]. 《时珍国医国药》, 2012,23(9)2182-2183.参考文献有19篇,其中最早的文献为1977年发表的。
(2)李行诺, 翟文丰, 周孟宇, 等. 黄柏化学成分研究[J].《浙江工业大学学报》,2012,40(3) 244-246.参考文献有9篇,其中最早的文献为1980年发表的。
(3)刘仁俊. 黄柏化学成分及药理作用浅谈[J].《中国中医药现代远程教育》,2011,9(13)83-84 .参考文献有10篇,其中最早的文献为1995年发表的。
(4)于滢. 黄柏的药理作用及应用[J]. 《养殖技术顾问》, 2011,(8)239-239 .参考文献有10篇,其中最早的文献为2006年发表的。
(5)杨周平, 武志军. 中药黄柏的药理作用和临床应用研究[J].《甘肃医药》,2010,29(3) 329-331.参考文献有7篇,其中最早的文献为1982年发表的。
(6)郑顺亮, 李开通,杨帅, 等. 清络方活性部位的化学成分研究[J]. 《中成药》,2010,32(10) 1753-1757.参考文献有18篇,其中最早的文献为1989年发表的。
(7)祈东利, 贾天柱, 廉莲. 黄柏炮制后化学成分转化研究[J]. 《中成药》,2010,32(3)443-447 .参考文献有6篇,其中最早的文献为1981年发表的。
八年级英语议论文论证方法单选题40题1. In the essay, the author mentions a story about a famous scientist to support his idea. This is an example of _____.A.analogyB.exampleparisonD.metaphor答案:B。
本题主要考查论证方法的辨析。
选项A“analogy”是类比;选项B“example”是举例;选项C“comparison”是比较;选项D“metaphor”是隐喻。
文中提到一个关于著名科学家的故事来支持观点,这是举例论证。
2. The writer uses the experience of his own life to prove his point. This kind of method is called _____.A.personal storyB.example givingC.case studyD.reference答案:B。
选项A“personal story”个人故事范围较窄;选项B“example giving”举例;选项C“case study”案例分析;选项D“reference”参考。
作者用自己的生活经历来证明观点,这是举例论证。
3. The author cites several historical events to strengthen his argument. What is this method?A.citing factsB.giving examplesC.making comparisonsing analogies答案:B。
选项A“citing facts”引用事实,历史事件可以作为例子,所以是举例论证;选项B“giving examples”举例;选项C“making comparisons”比较;选项D“using analogies”使用类比。
The Power of Comparative AnalysisComparative analysis is a valuable tool that enables us to gain a deeper understanding of subjects by comparing and contrasting them. This approach is widely used in various fields, including literature, science, history, and even daily life.In literature, for instance, comparative analysis can be employed to compare and contrast different works of the same author, or works from different authors, periods, or genres. By doing so, readers can appreciate the unique stylistic choices, themes, and motifs that authors employ, and how they contribute to the overall effect of the work.In science, comparative analysis is equally important. Scientists often compare and contrast different species, theories, or experiments to gain insights into the natural world. By identifying similarities and differences, they can make informed predictions, test hypotheses, and refine their understanding of scientific phenomena.In daily life, we also make use of comparative analysis unconsciously. For example, when shopping for a new car, we might compare and contrast different models, brands, and features to find the best fit for our needs. Similarly, when considering career options, we might compare job requirements, salaries, and potential growth opportunities to make an informed decision.The power of comparative analysis lies in its ability to help us see patterns, trends, and connections that might not be apparent at first glance. By comparing and contrasting different subjects, we can gain a more comprehensive understanding of the world and make more informed decisions. Whether in literature, science, or daily life, comparative analysis is a critical skill that can enhance our understanding and appreciation of the world around us.对比法是一种有价值的工具,它使我们能够通过比较和对比不同的事物来更深入地理解它们。
2023年教师资格之中学英语学科知识与教学能力题库及精品答案单选题(共100题)1、请阅读Passage 2,完成第小题。
A.hoaxB.hard workC.irksome taskD.special mission【答案】 A2、 Listening to loud music at rock concerts__________caused hearing loss in some teenagers.A.isB.areC.hasD.have【答案】 C3、Evidence came up ____ specific sounds are recognized by babies as young as six months old.A.whatB.thatC.whichD.whose【答案】 B4、Which of the following is closest in meaning to the underlined word "ambient" in Paragraph 2?A.SurroundingB.FamiliarC.ForeignD.Local【答案】 A5、Without your help, we would not __________ the work in time.A.have completedB.had completedpletedpleting【答案】 A6、Which of the following about teaching assessment isinappropriate?A.Diagnostic assessment is a kind of test carried out after a teaching activityB.Formative assessment should be student-orientedC.Summative assessment is often carried out at the end of a termD.Portfolios,daily reports,and delivering speeches are all formative assessment【答案】 A7、Which of the following activities does not belong topre-reading activities?A.scanningB.setting the sceneC.skimmingD.paraphrasing【答案】 D8、Everyone knows that English departments are in trouble, but you can't appreciate just howmuch trouble until you read the new report from the Modern Language Association. The report is about Ph.D. programs, which have been indecline since 2008. These programs have gotten both more difficult and less rewarding: today, it can take almost a decade to get a doctorate, and, at the end of your program, you're unlikely to find a tenure-track job.A.The idea of designing a shorter programB.The completion of a degree in five yearsC.The idea of drafting a shorter dissertationD.The suggestions given in the M.L.A. report【答案】 A9、请阅读Passage l。
硫必利与利培酮治疗阿尔茨海默病的对比研究高洁【摘要】Objective:To compare the curative effect between tiapride and risperidon on Alzheimer disease.Methods:100 patients with Alzheimer disease in our hospital were randomly divided into two groups :tiapride group and risperidone group , 50 cases in each group.Tiapride group were given tiapride tablets alone while risperidone group were given risperidone tablets alone .The clinical efficacy was compared between the two groups.Results: The tiapride group and the risperidone group had no significant differences in PANSS and BAHAVE-AD scores before treatment ( P>0.05).PANSS and BAHAVE-AD scores in both groups in two weeks , four weeks and eight weeks after treatment were significantly different from those before treatment ( P<0.05).PANSS score between the two groups were significantly different two and four weeks after treatment ( P <0.05 ).BAHAVE-AD score between the two groups were significantly different two weeks after treatment ( P<0.05).According to TESS scale, side effects in the two groups were mainly akathisia , tremor, muscle tension, tachycardia, hypotension, lethargy and constipation situation.The tiapride group demonstrated less side effects ,and akathisia, tremor and muscle tension were significant lower than those in the risperidone group ( P<0.05 ).Conclusion: Both tiapride and risperidone is effective for Alzheimer disease.Tiapride has rapid curative effect and low side effect compared with risperidone , but their long-term effects are almost similar.%目的:比较硫必利和利培酮治疗阿尔茨海默病精神行为症状的临床疗效。
Phytomedicine20 (2013) 187–193Contents lists available at SciVerse ScienceDirectPhytomedicinej o u r n a l h o m e p a g e:w w w.e l s e v i e r.d e/p h y m edComparison the neuropreotective effect of Cortex Phellodendri chinensis and Cortex Phellodendri amurensis against beta-amyloid-induced neurotoxicity in PC12cellsYan-Fang Xian a,Zhi-Xiu Lin a,Siu-Po Ip a,∗,Zi-Ren Su b,Jian-Nan Chen b,Xiao-Ping Lai b,∗∗a School of Chinese Medicine,The Chinese University of Hong Kong,Shatin,N.T.,Hong Kong Special Administrative Regionb College of Chinese Medicines,Guangzhou University of Chinese Medicine,Guangzhou,PR Chinaa r t i c l e i n f oKeywords:Cortex Phellodendri chinensis Cortex Phellodendri amurensis NeuroprotectiveBeta-amyloidPC12cells a b s t r a c tCortex Phellodendron chinensis(CPC)and Cortex Phellodendron amurensis(CPA)derived from the dried bark of Phellodendron chinense Schneid.or Phellodendron amurense Rupr.,respectively,are used inter-changeably in clinical practice under the name“Huang Bai”for centuries in Chinese medicine for the treatment of various inflammatory conditions.Previous study in our laboratory demonstrated that CPC and CPA had different anti-diarrheal,anti-bacterial and anti-inflammatory effects.In this present study, we aimed to compare the protective effect of ethanol extract of Cortex Phellodendri chinensis(ECPC)and Cortex Phellodendri Amurensis(ECPA)against beta-amyloid(A)-induced neurotoxicity in PC12cells, a typical model of Alzheimer’s disease.The results showed that ECPC and ECPA contain four common chemical markers such as berberine,but palmatine and jatrorrhizin were not found in CPC in contrast to the presence in CPA.In addition,both ECPC and ECPA can significantly increase the cell viability in A-treated PC12cells.Moreover,ECPC and ECPA can markedly elevate the ratio of the protein and mRNA levels of Bcl-2/Bax,while remarkably decrease the release of cytochrome c,and the protein and mRNA expression of caspase-3.Interestingly,ECPA has better protective effect than ECPC against A-induced neurotoxicity in PC12cells.These results indicate that both ECPC and ECPA have potential protective effect against A-induced neurotoxicity in PC12cells,and ECPA is more potential of the two species to be used in traditional medicine as a neuroprotective agent for the treatment of AD.The neuroprotective effect of the two species may be mediated,at least in part,via suppressing of the cellular apoptosis.© 2012 Elsevier GmbH. All rights reserved.IntroductionAlzheimer’s disease(AD)is an age-related neurodegenera-tive disorder afflicting an estimated30million people worldwide (Carnini et al.2006).However,the cause of AD is not well under-stood and the treatment is therefore only palliative(Mohajeri and Leuba2009;Savioz et al.2009).Beta-amyloid(A),the major pro-tein component of senile plaques,has been considered to play an important role in the development and progression of AD(Hardy Abbreviations:AD,Alzheimer’s disease;A,Beta-Amyloid;CP,Cortex Phel-lodendri;CPA,Cortex Phellodendron Amurensis;NGF,nerve growth factor;MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide;DMEM,Dulbecco’s modified eagle medium;FBS,fetal bovine serum;HPLC,high-performance liq-uid chromatography;ECPC,ethanol extracts of Cortex Phellodendri Chinensis; ECPA,ethanol extracts of Cortex Phellodendri Amurensis;ELISA,enzyme-linked immunosorbent assay;RT-PCR,real time-polymerase chain reaction.∗Corresponding author.Tel.:+852********;fax:+852********.∗∗Corresponding author.Tel.:+862039358517;fax:+862039358390.E-mail addresses:paulip@.hk,xianyan1981@(S.-P.Ip), lxp88@(i).1997).Apoptosis in neurons may contribute to the neuronal degen-eration in AD.Accumulation of evidences have demonstrated that Acan elicit neurons apoptosis in vitro and in vivo(Li et al.2010; Liu et al.2010),though the precise mechanisms underlying A-induced neurotoxicity remains unclear.As a result,any substances which can prevent A-induced apoptosis could modulate Atox-icity and improve neurological outcomes in AD.Cortex Phellodendri(CP),known as“Huang Bai”in Chinese,is commonly used in Chinese medicine to remove damp heat,quench fire,counteract toxicity,relieve consumptive fever and also effec-tive in curing dysentery,diarrhea and other syndromes(Yen1994). There are two species known as CP using in clinical practice,Cortex Phellodendron Chinensis(CPC)derived from the dried bark of Phel-lodendron chinense Schneid.,and Cortex Phellodendron amurensis (CPA)derived from the dried bark of Phellodendron amurense Rupr. (Chinese Pharmacopoeia Commission2010).Because of the similar constituents such as berberine,plamitine,phellodendrine,jatror-rhizine,obakulactone,obacunone,hyperin,and phellozide,the two species are used interchangeably in clinical applications in Chinese medicine.Thus,to better use of the two related species,a compar-ison of their pharmacological activities is urgently needed.0944-7113/$–see front matter© 2012 Elsevier GmbH. All rights reserved. /10.1016/j.phymed.2012.09.028188Y.-F.Xian et al./Phytomedicine20 (2013) 187–193Previous study in our laboratory demonstrated that CPC and CPA had different anti-diarrheal,anti-bacterial and anti-inflammatory effects(Chen et al.2010;Xian et al.2011).Recent studies revealed that CPA had neuroprotective effect(Park et al.2007;Jung et al. 2009).In addition,CP is also an important component herb of many popular herbal formulae,such as Huanglian Jiedu Decoction and Yiqi Congming Decoction,for the treatment of AD(Ma and Wang 2011;Xiao and Zhang2011).Therefore,we hypothesize that CPC and CPA may have different neuroprotective effect.In this present study,we endeavored to compare the neuroprotective effects of the ethanol extract of CPC and CPA against A-induced neurotoxicity in PC12cells.Materials and methodsReagents and chemicalsNerve growth factor(NGF),the fragment of-Amyloid peptide (A25–35),3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)were purchased from Sigma–Aldrich(St.Louis,MO, USA).Dulbecco’s modified Eagle Medium(DMEM),fetal bovine serum(FBS),penicillin and streptomycin were purchased from Gibco(Grand Island,NY,USA).All other reagents and chemicals used in the study were of analytical grade.Plant materialsThe samples of Cortex Phellodendri chinensis(CPC)and Cor-tex Phellodendri amurensis(CPA)were obtained from the national institute for the control of Pharmaceutical and Biological products, Beijing,China.They were authenticated by Ms Yu-ying Zong of our school.Voucher specimens(HB-01-12)have been deposited at the School of Chinese Medicine,The Chinese University of Hong Kong. Preparation of ethanol extracts of Cortex Phellodendri chinensis (ECPC)and Cortex Phellodendri amurensis(ECPA)The herbal materials were extracted as previously described (Xian et al.2011).Briefly,the dried plant materials of CPC(100g) or CPA(100g)were cut into small pieces and refluxed with 800ml ethanol for1h.Afterfiltration,the extraction procedure was repeated twice.The pooled extracts were combined and con-centrated under reduced pressure,followed by freeze drying.The yields of the extracts were approximately14%(w/w)in both species.High-performance liquid chromatographic(HPLC)analysisThe HPLC analysis of PCC and PAC were determined as described previously with minor modifications(Chinese Medicine Division 2005).Briefly,the extracts were dissolved in ethanol(20mg/ml) and injected into HPLC(Agilent1100Series)by autosampler.A Waters Nova-Pak C18HPLC column(3.9mm×300mm)was used for the separation.The mobile phase consisted of0.1%(v/v)tri-fluoroacetic acid in water(A)and acetonitrile(B).Separation was achieved by a linear gradient elution from0–50%B in0–48min, 50–100%B in48–55min and100%B in55–60min at aflow rate of 0.8ml/min.The separation temperature was set at room tempera-ture and the detection wavelength was346nm.Preparation of aggregated Aˇ25–35A25–35was dissolved in deionized distilled water at a con-centration of1mM and incubated at37◦C for4days to induce aggregation(Xian et al.2012).After aggregation,the solution was stored at−20◦C until use.Cell culture and drugs treatmentThe PC12cells were obtained from the American Type Culture Collection(Rockville,MD,USA).They were maintained in DMEM medium supplemented with penicillin(100U/ml),streptomycin (100g/ml),6%FBS,and6%horse serum at37◦C in a humidified atmosphere of95%air and5%CO2,and allowed to adhere for24h at 37◦C with the culture medium.PC12cells were differentiated with 50ng/ml NGF in serum-free DMEM for3days(Xian et al.2012). Thereafter,the culture medium was replaced by fresh serum-free DMEM(without NGF)with or without different concentrations of ECPC and ECPA(final concentrations:0.1and1g/ml)for2h.Then 20M of A25–35was added to the cells and incubated for another 24h.The extracts and isolated compounds were reconstituted in DMSO to produce respective stock solutions and then diluted with culture medium to various concentrations for cell culture experi-ments.Thefinal DMSO concentration in each sample was less than 0.1%.Cell viability assayCell viability in the presence of ECPC and ECPA was mea-sured by quantitative colorimetric assay with MTT method as described previously(Xian et al.2012).Briefly,after drug treat-ment,20l/well of MTT solution(final concentration,1mg/ml) was added and cells were incubated at37◦C for4h.The supernatants were then aspirated off and formazan crystals were dissolved with150l of DMSO.The optical density of each well was determined at570nm using a FLUOstar OPTIMA microplate reader(BMG Labtech,Offenbury,Germany). Cell viability was expressed as percentage of the non-treated control.Quantification of DNA fragmentationQuantification of DNA fragmentation was determined by Cell Death Detection ELISA Plus kit(Roche Applied Sciences,Basel, Switzerland)as per the manufacturer’s protocol.In brief,the cells were washed with D-Hanks solution after drug treatment.Then the cells were incubated with200l of lysis buffer for30min at room temperature.The plate was centrifuged at200×g for 10min at4◦C.An aliquot(20l)of the supernatant from each well was transferred to a streptavidin-coated microplate and incubated with a mixture of anti-histone-biotin and anti-DNA-peroxidase.The apoptotic nucleosomes were captured via their histone component by the anti-histone-biotin antibody which was bound to the streptavidin-coated microplate.Simultaneously,anti-DNA-peroxidase was bound to the DNA part of the nucleosomes. After removing the unbound antibodies,the amount of peroxidase retained in the immunocomplex was quantified by adding2,2 -azinobis(3-ethylbenzthiazoline-6-sulphonic acid)(ABTS)as the substrate,and the absorbance of the reaction mixture was mea-sured at405nm using a microplate reader.The absorbance is directly proportional to the number of apoptotic nucleosomes.The extent of DNA fragmentation was expressed as percentage of the non-treated control.Cytochrome c assayThe PC12cells were seeded onto6-well plates at2×106 cells/well.The cells were washed twice with D-Hanks solution after drug treatment.Cells were collected,fractionated,and cytosolic cytochrome c was measured using the Quantikine M rat/mouseY.-F.Xian et al./Phytomedicine20 (2013) 187–193189cytochrome c Enzyme-linked immunosorbent assay(ELISA)kit (R&D systems,Minneapolis,MN,USA)according to the manu-facturer’s instruction.Briefly,cytosolic fractions were added into a microplate precoated with rat/mouse cytochrome c.After2-h incubation and washing,substrate solution was added to each well.The reaction was stopped after30min and the optical den-sity was measured with a microplate reader at490nm.The level of cytochrome c was expressed as percentage of the non-treated control.Western blotting analysisThe PC12cells were seeded onto100mm2dish at5×106 cells/dish.The cells were washed twice with D-Hanks solution after drug treatment.The cells were harvested and lysed with protein lysis buffer.Protein samples were electrophoresed by SDS-PAGE for2h at80V.The separated proteins were transferred to PVD membranes using a transblotting apparatus(Bio-Rad Labo-ratories,USA)for30min at15V.The membranes were blocked with5%(w/v)non-fat milk in TBS-T(Tris-buffer saline contain-ing0.1%Tween-20)at room temperature for2h and subsequently incubated at4◦C overnight with appropriate amount of primary antibody against Bcl-2(1:1500),Bax(1:1500),caspase-3(1:1000), and-actin(Santa Cruz Biotechnology Inc.,USA).Next,the mem-brane was washed with TBS-T three times and probed with horseradish peroxidase conjugated secondary antibody at room temperature for1h.To verify equal loading of samples,the mem-branes were incubated with monoclonal antibody-actin,followed by a horseradish peroxidase conjugated goat anti-mouse IgG.The membrane again was washed with TBS-T for three times andfinally the protein bands were visualized by the ECL western blotting detection reagents(Amersham Biosciences,Buckinghamshire,UK). The intensity of each band was analyzed using Image J software (NIH Image,Bethesda,MD,USA).Real-time polymerase chain reaction(RT-PCR)analysisThe PC12cells were seeded onto100-mm2dish at5×106 cells/dish.The cells were washed twice with D-Hanks solution after drug treatment.Total RNA was isolated from the cells with TRI-zol Reagent(Gibco,Grand Island,NY,USA).The concentration of extracted RNA was measured spectrophotometrically at260nm. The quality of RNA was assessed by the ratio of absorbance at260 and280nm.The values of A260/A280from1.9to2.1were con-sidered as acceptable.Total RNA(1.5g)was used to synthesize cDNA using High capacity cDNA reverse transcription kit(Applied Biosystems,Foster City,CA,USA)following the manufacturer’s instructions.We performed real-time PCR with reagents and pro-tocols from TaqMan fast universal PCR master mix kit(2×)(Applied Biosystems,Foster City,CA,USA)and mouse TaqMan gene expres-sion assays(Applied Biosystems,assay ID:Bcl-2,Rn99999125m1; Bax,Rn02532082g1;caspase-3,Rn00563902m1;and-actin, Rn00667869m1).The reactions were run at50◦C for2min,10min at95◦C,followed by60cycles at95◦C for15s and60◦C for 1min on Applied Biosystems step-one Fast Real-Time PCR sys-tem.Sequence Detection Software2.0(Applied Biosystems,Inc., FosterCity,CA,USA)was used for data analysis.The relative expres-sion of Bcl-2and Bax mRNA were normalized to the amount of -actin in the same cDNA using the relative quantification method (2− CT method).The fold change in target gene cDNA relative to the-actin internal control was determined by the following formula:Fold change=2 CT,where CT=(Ct target gene−Ct-actin)−(Ct control−Ct-actin)Statistical analysisData were expressed as mean±SEM.Multiple group com-parisons were performed using one-way analysis of variance (ANOVA)followed by Dunnett’s test in order to detect inter-group differences.For non-parametric data,Kruskal–Wallis test was conducted.GraphPad Prism software was used to perform the statistical analysis(Version4.0;GraphPad Software,Inc.,San Diego, CA).A difference was considered statistically significant if the p value was less than0.05.ResultsThe results of HPLC analysesExamination of the HPLC chromatograms of CPA(Fig.1A)and CPC(Fig.1B)showed that they contain four common chemical markers,pounds1–4.However,compound5(palmatine) and compound6(jatrorrhizin)were not found in CPC in con-trast to the presence in parison of these peaks was made on the basis of their ultraviolet absorption spectra and retention times,which indicated that the contents of compounds1,3(mag-noflorine)and4(berberine)in CPC were about7,3and10times higher that those in CPA.CPC and CPA contained the same content of compound2.On the other hand,the level of palmatine and jatror-rhizine were found to be low,almost undetectable in CPC.Although the content of berberine was much low in CPA,both plamatine and jatrorrhizine could be detected in CPA.Effect of ECPC and ECPA on Aˇ25–35-induced cytotoxicity in PC12 cellsAs shown in Fig.2,ECPC and ECPA at the concentrations up to 1g/ml did not show significantly cytotoxicity to PC12cells when incubated for24h.Treatment of PC12cells with20M of A25–35 for24h induced approximately45%cell death.When the cells were pretreated with ECPC(1g/ml)for2h significantly increased the cell viability(p<0.001)in A25–35-treated PC12cells,as compared with the A25–35-treated control group.On the other hand,pre-treatment with ECPA(0.1and1g/ml)for2h also significantly increased the cell viability(p<0.001and p<0.001,respectively)in A25–35-treated PC12cells,as compared with the A25–35-treated control group.Interestingly,ECPA more powerfully increased the cell viability than ECPC at1g/ml(p<0.05).Effect of ECPC and ECPA on DNA fragmentation in Aˇ25–35-treated PC12cellsAs shown in Fig.3,treatment of PC12cells with20M of A25–35for24h induced significantly increase of DNA fragmen-tation(2.0-fold),as compared with the control group.When the cells were pretreated with ECPC(0.1and1g/ml)for2h,the DNA fragmentation was significantly decreased(p<0.001and p<0.001, respectively)in A25–35-treated PC12cells,as compared with the A25–35group.On the other hand,pretreatment with ECPA(0.1 and1g/ml)for2h,the DNA fragmentation was also significantly decreased(p<0.001and p<0.001,respectively)in A25–35-treated PC12cells,respectively,as compared with the A25–35group.Inter-estingly,ECPA more powerfully inhibited the DNA fragmentation than ECPC at1g/ml(p<0.01).Effect of ECPC and ECPA on cytochrome c in Aˇ25–35-treated PC12 cellsAs shown in Fig.4,treatment of PC12cells with20M of A25–35 for24h induced significantly increase the released of cytochrome190Y.-F.Xian et al./Phytomedicine 20 (2013) 187–193min1020304050mAU 0102030404(B)123min1020304050mAU 02.557.51012.51517.5654(A)123Fig.1.High-performance liquid chromatography profile of Cortex Phellodendri amurensis (A)and Cortex Phellodendri Chinensis (B):3:magnoflorine;4:berberine;5:palmatine;6:jatrorrhizine.c (3.0-fold),as compared with the control group.When the cells were pretreated with ECPC (0.1and 1g/ml)for 2h,significantly inhibited the release of cytochrome c (p <0.001and p <0.001,respectively)in A 25–35-treated PC12cells,as compared with the A 25–35group.On the other hand,pretreatment with ECPA (0.1and 1g/ml)for 2h,significantly decreased the release of cytochrome cFig.2.Effect of ECPC and ECPA on A 25–35-induced cytotoxicity in PC12cells.Val-ues given are the mean ±SEM (n =6).#p <0.001compared with the control group;*p <0.05,***p <0.001compared with the A 25–35-treated group;&p <0.05as compare to the A 25–35plus ECPA 1g/ml.(p <0.001and p <0.001,respectively)in A 25–35-treated PC12cells,as compared with the A 25–35group.Interestingly,ECPA more powerfully reduced the released of cytochrome c than ECPC at 1g/ml (p <0.05).Effect of ECPC and ECPA on the protein and mRNA expression of the ratio of Bcl-2/Bax in A ˇ25–35-treated PC12cellsFig.5shows the effect of ECPC and ECPA on the ratio of protein (Fig.5A)and mRNA (Fig.5B)expression of Bcl-2/Bax in A 25–35-treated PC12cells.Treating PC12cells with 20M of A 25–35for 24h caused a significant decrease of the ratio of protein (0.39-fold)and mRNA (0.42-fold)expression of Bcl-2/Bax,as compare with the control group.Pretreated with ECPC (0.1and 1g/ml)signifi-cantly increased the protein (p <0.05and p <0.05,respectively)and mRNA (p <0.01and p <0.01,respectively)expression of Bcl-2/Bax,as compared with the A 25–35group.On the other hand,pretreated with ECPA (0.1and 1g/ml)also significantly elevated the pro-tein (p <0.05and p <0.05,respectively)and mRNA (p <0.01and p <0.01,respectively)expression of Bcl-2/Bax,as compared with the A 25–35group.Effect of ECPC and ECPA on the protein and mRNA expression of caspase-3in A ˇ25–35-treated PC12cellsFig.6shows the effect of ECPC and ECPA on the protein (Fig.6A)and mRNA (Fig.6B)expression of caspase-3in A 25–35-treated PC12cells.A 25–35stimulation significantly increased the protein (1.6-fold)and mRNA (1.8-fold)expression of caspase-3in A 25–35-treated PC12cells,as compare to the control group.Pretreated with ECPC (0.1g/ml)significantly increased the protein expres-sion of caspase-3(p <0.01),as compared with the A 25–35group.On the other hand,pretreatment with ECPA (0.1and 1g/ml)signifi-cantly suppressed the protein (p <0.001and p <0.001,respectively)and mRNA (p <0.001)expression of casapse-3,as compare to theY.-F.Xian et al./Phytomedicine20 (2013) 187–193191Fig.3.Effect of ECPC and ECPA on the extent of DNA fragmentation in A25–35-treated PC12cells.Values given are the mean±SEM(n=6).#p<0.001compared with the control group;**p<0.01and***p<0.001compared with the A25–35-treated group;&p<0.01as compare to the A25–35plus ECPA1g/ml.A25–35group.Interestingly,ECPA more powerfully inhibited theprotein and mRNA expression of caspase-3than ECPC at1g/ml(p<0.05).DiscussionApoptosis is a morphologically and biochemically process ofcell death that occurs via activation of distinct signaling pathwaysinvolving mitochondria,mitochondrial regulatory proteins,andactivation of caspase members(Cui et al.2005).Recent researchescorroborated that the apoptosis induced by Avia altering theexpression of the Bcl-2family of apoptosis-related genes(Yao et al.2005)and survival signaling pathways are required for protec-tion of A-mediated neuronal apoptosis(Di Segni et al.2005).Bcl-2family members,such as Bcl-2and Bax,are the major reg-ulators of the permeability of the mitochondrial membrane.Bcl-2,an anti-apoptotic protein,stabilizes mitochondrial membrane per-meability,thereby maintaining mitochondrial integrity,reducingthe release of cytochrome c and inhibiting apoptosis(Jang andSurh2004).On the other hand,Bax,pro-apoptotic proteins,affectsthe permeability of the mitochondrial membrane and induces therelease of cytochrome c from the inter-membrane space of themitochondrial into the cytosol,which subsequently leads to apo-ptosis(Kostic et al.1991;Yang et al.1998).In addition,the ratioof Bcl-2/Bax influences the release of cytochrome c and caspase-3activation(Gross et al.1999).Cytochrome c releases out of themitochondria,which ultimately lead to the activation of caspase-3(Bras et al.2005),and the activation of caspase-3can induceDNA fragmentation,nuclear chromatin condensation andcellFig.4.Effect of ECPC and ECPA on cytochrome c in A25–35-treated PC12cells.Values were given are the mean±SEM(n=6).#p<0.001as compared to the control group; ***p<0.001as compare to the A25–35-treated group;&p<0.05as compare to the A25–35plus ECPA1g/ml.apoptosis(Li et al.2008).Moreover,overproduction of caspase-3has been observed in the neurons of the brain of AD patients (Wai et al.2009).Therefore,the balance of the ratio of Bcl-2/Bax plays a critical role in the early phases of the apoptotic cascade.In this present study,our results demonstrated that A25–35signifi-cantly increased the cell death,the extent of DNA fragmentation, the release of cytochrome c and caspase-3expression,while atten-uated the ratio of Bcl-2/Bax in PC12cells.All these observations are consistent with other research reports(Li et al.2008).However, pretreatment with ECPC and ECPA could reverse these changes induced by Ain PC12cells.In this present,we have found that the ECPA(lower content of berberine)is more potential protective effect against A-induced neurotoxicity in PC12cells that ECPC(higher content ofberberine). Fig.5.Effect of ECPC and ECPA on the ratio of protein(A)and the mRNA expression (B)of Bcl-2/Bax in A25–35-treated PC12cells.Values were given are the mean±SEM (n=3–6).#p<0.001as compared to the control group;*p<0.05and**p<0.01as compare to the A25–35-treated group.192Y.-F.Xian et al./Phytomedicine 20 (2013) 187–193Fig.6.Effect of ECPC and ECPA on the protein (A)and the mRNA expression (B)of the caspase-3in A 25–35-treated PC12cells.Values were given are the mean ±SEM (n =3–6).#p <0.001as compared to the control group;**p <0.01and ***p <0.001as compare to the A 25–35-treated group;&p <0.05as compare to the A 25–35plus ECPA 1g/ml.Although the exact reasons underlying the different neuroprotec-tive effect of ECPC and ECPA are not well understood,the different components of the two species may play an important role.Berber-ine,the major active ingredient of CP (Fukuda et al.1999;Kuo et al.2004),had been reported to possess the potential neuropro-tective effect against mental depression,schizophrenia,cerebral ischemia,anxiety,and AD (Ye et al.2009;Kulkarni and Dhir 2010;Ji and Shen 2011).In addition,berberine also has been demon-strated to possess the anti-AD effects of through inhibition of acetyl and butyl cholinesterase,indoleamine 2,3-dioxygenase and A 1–42fibril formation,amelioration of A 1–40-induced cognitive impairments (Jung et al.2009;Shi et al.2011;Yu et al.2010;Zhu and Qian 2006),and attenuation amyloid plaque production in Swedish amyloid precursor protein-expressing cells (Asai et al.2007).Moreover,recent research indicated that berberine could ameliorate A pathology,gliosis,and cognitive impairment in an AD transgenic mouse model (Durairajan et al.2012).Previous and recent studies showed that CPC had higher content of berberine (Chinese Medicine Division 2005;Chan et al.2007).Besides berber-ine,other chemical substrates such as palmatine,phellodendrine,jatrorrhizine,magnoflorine and obakulactone were the common ingredients existing in both species.The content of phellodendrine and magnoflorine in CPC was higher than that in CPA,while the content of palmatine and jatrorrhizine in CPA was obviously higher than that in CPC (Chan et al.2007;Wagner et al.2011).Among them,berberine,palmatine and jatrorrhizine have potential anti-AD effects (Jung et al.2009).The chemical ingredients contained in the extract of CP may have synergistic action among them in the neuroprotective effect.In summary,our results demonstrated that both ECPC and ECPA have protective effect against A -induced neurotoxicity in PC12cells.The neuroprotective effect of ECPC or ECPA may be mediate,by suppressing of the cellular apoptosis.Interestingly,ECPA is more potential of the two species to be used in traditional medicine as an anti-AD agent.The obtained findings provide a further pharma-cological basis for the therapeutic efficacy of the extract of CP for treatment of progressive neurodegenerative disease.Further study need to determine the neuroprotective mechanism and identify the active components of the two species.Moreover,as a result of the similar components of the different species or subspecies,com-parative investigation of the different species,or subspecies of one Chinese herbal drug is a basic requirement for the pharmacological valuation and the therapeutic efficacy.AcknowledgmentThe plant materials used in this study were supplied by the Hong Kong Chinese Material Medica Standards project supported by the Department of Health,Hong Kong Special Administrative Region Government.ReferencesAsai,M.,Iwata,N.,Yoshikawa,A.,Aizaki,Y.,Ishiura,S.,Saido,T.C.,Maruyama,K.,2007.Berberine alters the processing of Alzheimer’s amyloid precursor protein to decrease Abeta secretion.Biochemical and Biophysical Research Communi-cations 352,498–502.Bras,M.,Queenan,B.,Susin,S.A.,2005.Prohrammed cell death via mitochondria:different modes of dying.Biochemistry 70,231–239.。