tm-100使用说明书

Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 What’s in the Box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 Printer Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 Reliability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 Printing a Status Sheet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 Additional Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 - TM-m10 and TM-m30 POS Receipt PrinterParts From left to right:❏USB Type A connector (TM-m30 only; for customerdisplay and Wireless LAN unit)❏Power connector❏USB Type B connector (for host device)❏Ethernet connector (depending on model)❏DK (Drawer kick) connectorWhat’s in the Box❏Printer❏User’s manual and setup guide❏Small thermal paper roll (for operational testing)❏AC adapter ❏AC cable❏USB cable (depending on model)❏Locking wire saddle (depending on model)❏58-mm roll paper guides (TM-m30)AccessoriesPrinter SpecificationsPrintingPrint method:thermal line printing Dot density:203 dpi × 203 dpiPrint width:TM-m10:2.07" (52.5 mm), 420 dots TM-m30:2.07" (52.5 mm), 420 dots 2.82" (72 mm), 576 dotsCharacters per line:58mm roll: (standard)font A: 35, font B: 42, font C: 4680mm roll (TM-m30):font A: 48, font B: 57, font C: 64Print direction:TM-m10: unidirectional w/friction feed TM-m30: bidirectional w/friction feed Print speed:TM-m10: 5.9"/sec. (150 mm/sec.) max.TM-m30: 7.9"/sec. (200 mm/sec.)max.Continuous paper feed speed:TM-m10:approx. 5.9"/sec. (150 mm/sec.)TM-m30:approx. 7.9"/sec. (200 mm/sec.)Character sets:alphanumeric: 95 characters international: 18 setsextended graphics: 128 × 43 pages (including a user-defined page)InterfacesUSB model (TM-m10):Built-in USB 2.0 Full-speed Ethernet model:Built-in USB 2.0 Full-speed and Ethernet 10/100 Base-T/TXBluetooth ® model:Built-in USB 2.0 Full-speed, Bluetooth3.0 (EDR supported), and Ethernet (TM-m30)Wi-Fi model:Built-in USB 2.0 Full-speed,802.11b/g/n (2.4 GHz), and Ethernet1Roll paper cover 2Panel LED 3NFC tag 4Cover open lever5Feed button 6Power switch7Autocutter adjustment knob (TM-m10 only)8Status sheet buttonOptionModel Part number External buzzer unitOT-BZ20C32C890634MediaPaper type:thermal roll paperPaper width:TM-m10:58 mm: 57.5 ± 0.5 mm (2.26 ± 0.02")TM-m30:58 mm: 57.5 ± 0.5 mm (2.26 ± 0.02")80 mm: 79.5 ± 0.5 mm (3.13 ± 0.02") Max. diameter:TM-m10:60.0 mm (2.36") outside maximum18.0 mm (0.71") outer core minimumTM-m30:83.0 mm (3.27") outside maximum18.0 mm (0.71") outer core minimum Specified paper:TM-m10:TM-m30:ReliabilityPrinter mechanism:15 million lines (printing and paper feed) Thermal head:100 million pulses, 100 kmMTBF:360,000 hoursMCBF:60 million linesAutocutter:TM-m10: 1 million cutsTM-m30: 1.5 million cuts ElectricalOperating voltage:DC 24V ± 7%Power consumption:TM-m10:15.9 W (AC 115V/60 Hz) (operating)0.34 W (AC 115V/60 Hz) (standby)TM-m30:26.6 W (AC 115V/60 Hz) (operating)0.41 W (AC 115V/60 Hz) (standby) Current consumption:1.5 A (operating)Approximately 0.1 A (standby)SafetyElectromagnetic interference:USA:FCC Class ACanada:CAN ICES-3 (A)/NMB-3 (A)EnvironmentalOperating temperature range:Temperature: 41 to 113°F (5 to 45°C)Humidity: 10 to 90% RH (except for paper) Storage temperature range:Temperature: -4 to 140°F (-20 to 60°C) Humidity: 10 to 90% RH (except for paper) Operating noise:approximately 55 dBDimensionsTM-m10Dimensions(H × W × D): 4 × 4 × 4 inches(102 × 102 × 102 mm)Weight:approx. 1.98 lb (0.9 kg)TM-m30Dimensions(H × W × D): 5 × 5 × 5 inches(127 × 127 × 127 mm)Weight:approx. 2.87 lb (1.3 kg)Paper type number ManufacturerKT55FA, KT48FA Koehler Paper GroupAF50KS-E Jujo Thermal Ltd.F5041(55)Mitsubishi HiTec PaperPaper type number ManufacturerKT55FA, KT48FA Koehler Paper GroupAF50KS-E Jujo Thermal Ltd.F5041(55), F5041(48)Mitsubishi HiTec PaperTM-m10 and TM-m30 POS Receipt Printer - 3Control PanelLEDs(Power)On when power is on.Flashing when printer is powering off; when updating firmware; when the Ethernet interface is starting up.ErrorOn when the printer is offline (paper out, power-on initialization, paper cover open) or other recoverable error. Flashing if there is an unrecoverable error.Turn the printer off for a few seconds, then turn the printer back on. If the LED is still blinking, contact a dealer or qualified service person.PaperOn when there is no paper in the printer.Flashing when waiting for the self-test or a macro execution; when printing the status sheet; when the firmware is being updated.ButtonsPowerPress this button to turn the power on or off. To turn the power off, hold the button for more than two seconds. FeedPress this button to advance the paper one line. Hold the button down to advance the paper.Status sheetPress this button to print an interface status sheet or initialize interface settings.Printing a Status SheetFollow the steps below to print a status sheet on roll paper. Using the status sheet button1.Make sure the printer is on and the roll paper cover is shut.2.Remove the bottom cover if necessary.3.Press and hold the status sheet button for at least 3 seconds.The printer prints its status.Using the Feed button1.Make sure the printer is on and the roll paper cover is open.2.Press and hold the Feed button for at least 3 seconds, thenclose the roll paper cover. The printer prints its status. Additional InformationThe Epson® support site has more information about the TM-m10 and TM-m30:/supportEPSON is a registered trademark and EPSON Exceed Your Vision is a registered logomark of Seiko Epson Corporation.General Notice: Other product names used herein are for identification purposes only and may be trademarks of their respective owners. Epson disclaims any and all rights in those marks.This information is subject to change without notice.© 2015 Epson America, Inc., 11/15CPD-50291R14 - TM-m10 and TM-m30 POS Receipt Printer。

IWORX 教学工具包从1991年,iWORX 开始进入教育领域，提供所有的生理学教育课程所需的工具包，产品包括: 数据采集硬件、专业的LabScribe 数据记录分析软件、齐全的换能器、信号调节器、各种附件。

每一种工具包可以提供针对人体、动物的心理生理学实验，IWORX 还可以提供创新的远程和异步网络教学系统。

人体生理教学工具包可以完成全面的实验课程，包括心血管、呼吸、神经肌肉等50套实验和180种练习.人体生理教学工具包 : 人体生理教学工具包可以完成全面的实验课程，包括心血管、呼吸、神经肌肉等50套实验和180种练习。

214四通道数据记录器LabScribe 数据记录分析软件 ECG/EMG/EEG 电缆（HK/214用) 刺激器电缆 BNC-BNC 电缆 USB 电缆12V 电源适配器 SP-300肺活量计 FT-325手提测力计 PT-100脉搏换能器 EM-100活动标记器 BP-700血压套件 TM-100温度传感器 150 Ag/AgCl 电极 使用说明书人体生理教学工具心理生理教学工具包: 可以完成大学要求的实验课程，包括脑电图、皮肤电阻、皮肤温度、心音及血压等实验。

214四通道数据记录器LabScribe 数据记录分析软件 ECG/EMG/EEG 电缆（HK/214用） BNC-BNC 电缆 USB 电缆12V 电源适配器 PT-100脉搏换能器 EM-100活动标记器 TM-100温度传感器GSR-200GSP(皮肤电反射)放大器 RM-200呼吸测量带 BP-600血压套件 可重复用脑电极 150 Ag/AgCl 电极 使用说明书心理生理教学工具包动物生理教学工具包: 可以完成全面的实验课程，包括新陈代谢、坐骨神经刺激、肌肉运动、离子运动和渗透平衡等11套实验和23种练习。

214四通道数据记录器LabScribe数据记录分析软件ECG/EMG/EEG电缆（HK/214用）刺激器电缆BNC-BNC电缆USB电缆12V电源适配器NBC-300神经浴槽五针神经浴槽记录电缆FT-104张力换能器DT-475位移换能器IC-200细胞内探针ISE-730刺激电极ISE-730氧电极PT-100脉搏换能器EM-100活动标记器FT-325手提测力计BP-700血压套件SP-300肺活量计TM-100温度传感器150 Ag/AgCl电极使用说明书动物生理教学工具包iWorx Physiology Teaching Kits have everything you need to make physi-ology labs exciting,informative and easy.They combine a rugged,eco-nomical data recording unit with click-and-play LabScribe software to provide versatile,cost-effective data recording solutions.They also include web-ready iWorx human,animal and psychological physiology courseware and a selection of trans-ducers and accessories needed for typical college-level lab experiments.Both the hardware and the software have been designed especially for teaching.Hardware components are easy to set up and virtually bScribe,a full featured recording and analysis application,actually configures itself for the experiment at hand,and allows a wide range of easy-to-perform analyses.iWorx ®Physiology T eaching KitsWeb-ready iWorx Courseware.The iWorx solution starts with dynamic,interactive human,animal and psycho-logical physiology courseware,developed over several years by university teaching professionals.The course materials include more than 50 lab experiments and 180 learning exercises.They’re 100% web-ready.You can easily put them on your own course home page,and customize the content to suit your own teaching requirements.Coursematerials are provided in an easily repro-ducible printed format,as well as .pdf and HTML files.The courseware works seamlessly with the LabScribe software.Selecting the group of experiments that come with the standard iWorx physiology lab manual adds over 40 experiments to the Settings sampling rate,calibrations,analysis func-tions,and more that are needed to com-plete the plete documentation for that lab is automatically called up and displayed in a separate window.A unique “settings manager”lets instructors attach external resources,such as Web links,.pdf files,movies or illustrations to the experimental settings file and makes them readily available to assist students in the completion of the experiment.Instructors can use the “settings manager”to customize their curricula as well.Web versions of the laboratory documen-tation feature professionally rendered,full-color graphics illustrating physiologi-cal principals.All of the web materials can be used locally,or placed on the course/department server for generalLabScribe makes even sophisticated experiments “click and play” easy. LabScribe has been developed from the ground up for classroom teaching,and strikes an ideal balance between flexi-bility and simplicity.Setup of gains, sampling rates,display times and other criteria is automatic via the “settings”file.No complicated adjustments are ers can easily control the various aspects of recording through dialogue windows and save their pref-erences for future use.In Manual mode,the user has control of all aspects of the recording.LabScribe includes its own function generator/stimulator for maximum experiment versatility.Easy-to-use tools let the student make measurements from the data with a minimum of manipulation.For example,LabScribe can display the Mean,Min,Max, Duration and Amplitude of a selection, or it can display the Rate or Integral of incoming signals in real time.Incoming voltages can be easily converted to real units such as pH or mmHg.And,of course,built-in online help is instantly available.Recorded data is presented as distance-per-unit of time,e.g.,cm/sec,exactly as it would be on a traditional paper chart ing the built-in annotation feature,students can even “write”on the virtual chart recording to quickly locate regions of interest.For convenience,professional-looking LabScribe reports can be prepared and edited,all within the program’s own Journal.If desired,the pictures and text in the Journal can be easily exported to other programs.iWorx Physiology TeachingKits are built around professionally-developed,web-ready coursewarethat includes human,animal and psychological physiology labs.Human Physiology Labs ⅢHeartⅢCirculationⅢExerciseⅢFluid BalanceⅢMusclesⅢNervesⅢRespirationAnimal Physiology LabsⅢCellular RespirationⅢMetabolismⅢMembrane PropertiesⅢNervesⅢMusclesⅢFluid BalanceⅢHeartⅢCirculationPsychophysiology LabsⅢBrain Waves and Cortical Arousal ⅢEmotion and Skin Conductance ⅢCognition and Skin Conductance ⅢBiofeedbackⅢVagal ToneⅢCynicism and Hostility LabScribe is a comprehensive, data recording and analysis solution developed especially for classroom use. It’s easy to use, yet offers a full range of features for human, animal and psychological physiology teaching.LabScribe’s Analysis window lets you zoom in on specific areas of the recording and perform calculations on selected pieces of the data.iWorx 214 Data Recorder This rugged,reliable 4-channel data recording unit is engineered to take years of classroom use and abuse.It is constructed of extruded aluminum, with a minimum of student-accessible controls and adjustments.For mostapplications,setup is automatic and transparent to the user.From within the software the unit can be configured for EEG,EMG (EOG) or ECG.The iWorx 214 includes a dual-channel biopotential amplifier approved for use on human subjects.Two additional channels accept practically any sensor you’re likely to encounter,as well as voltage output of pH meters,spectrophotometers and other laboratory devices.The iWorx 214also provides a fully functional built-instimulator useful for a variety of humanand animal labs.The recorder interfaces to a PC or MACvia a USB connection which providesaggregate sampling at 100 kHz continu-ous,more than fast enough for justabout any teaching experiment.System RequirementsThe iWorx Physiology Teaching Kitsrequire either a Pentium level computerrunning Windows 98,ME,2000,or XP,or a Macintosh computer running OSX.In either case the computer must haveat least 512 megabytes of RAM,and atleast 10 megabytes of free space on thehard drive.All necessary transducers, cables andconnectors are included in a ruggedstorage and carry case that keepseverything in its place and ready for use.Complete Package—Everything IncludediWorx Physiology Teaching Kits are shippedwith all transducers,cables,accessories,and manuals needed for the labs in theselected group—everything designed towork together.The transducers areextremely robust and carry the samethree-year warranty as the iWorx 214Data Recorder.Warranty and UpgradesiWorx Physiology Teaching Kit comes witha 3-year warranty.Software upgrades arefree,and are automatically delivered toregistered users via e-mail and can be eas-ily downloaded from the iWorx users area.LabScribe software and courseware maybe freely distributed to students.Analog Inputs: 2 isolated differential biopotential/2 single-ended2 differential transducer InputsInput Range:±5VExcitation Voltage:±5VInput Impedance:10 GohmAmplifier Response:0–2kHzAnalog Output: 1 (differential)Output Range:±5VOutput Current:5mAOutput Resolution:12 BitADC Resolution:16 BitSystem Noise:±1 LSB (< 1mV)Sampling Rate:100,000 samples/sec aggregate continuous Trigger 1(TTL)Biopotential Amplifier:Safe for use on human subjects;includes opticallyisolated input and AAMI input cableStimulator Output:±5V (accessories available to increase Stimulatoroutput to 100V) SpecificationsWant to learn more?Just call (800) 234-1757 or contact us ************************.One Washington Street,Suite 404Dover,New Hampshire 03820Phone:(800)-234-1757603)-742-2492 (International)Fax:(603)-742-2455©2006 CB Sciences,Inc.iWorx is a registered trademark and LabScribe is a trademark of CB Sciences,Inc.Other trade-Ordering InformationPart No.DescriptionHK/214Advanced Human Physiology Kit includes:ⅢiWorx 214 Four Channel Computer Interface with Built-In Two Channel Isolated Bioamplifier and LabScribe Recording Software ⅢSpirometer with 300 Liter Flow Head ⅢHand Dynamometer ⅢPulse Plethysmograph ⅢEvent MarkerⅢTemperature SensorⅢNon-Invasive Blood Pressure Transducer and Stethoscope Ⅲ150 Pre-Gelled Ag/AgCl Electrodes Ⅲ12VDC GND.Power Supply ⅢCablesⅢPrinted,HTML and PDF Lab ManualAHK/214Advanced Animal and Human Physiology Kit includes:ⅢiWorx 214 Four Channel Computer Interface with Built-In Two Channel Isolated Bioamplifier and LabScribe Recording Software ⅢSpirometer with 300 Liter Flow Head ⅢHand Dynamometer ⅢPulse Plethysmograph ⅢEvent MarkerⅢTemperature Sensor ⅢForce TransducerⅢDisplacement TransducerⅢNerve Bath Chamber with stimulating/recording Electrodes ⅢIntracellular ProbeⅢOxygen Electrode with Current-to-Voltage AdapterⅢNon-Invasive Blood Pressure Transducer and Stethoscope Ⅲ150 Pre-Gelled Ag/AgCl Electrodes Ⅲ12VDC GND.Power Supply ⅢCablesⅢDissection KitⅢPrinted,HTML and PDF Lab ManualPK/214Psychological Physiology Kit includes:ⅢiWorx 214 Four Channel Computer Interface with Built-In Two Channel Isolated Biopamplifier and LabScribe Recording Software ⅢPulse Plethysmograph ⅢEvent MarkerⅢTemperature SensorⅢNon-invasive Blood Pressure Transducer ⅢReusable Button ElectrodesⅢGalvanic Skin Response Amplifier ⅢCablesⅢ12VDC GND.Power SupplyⅢ150 Pre-Gelled Ag/AgCl Electrodes ⅢPrinted,HTML and PDF Lab ManualInnovation in Life Science Education。

CHERUSAL 型号: TM-100PR-HB脉冲热压机新加坡印制型号TM-100PR-HB操作维护手册目录第 1 章 : 产品概述 (1)加热方法 (1)机器部件和特性 (1)规格 (2)桌台尺寸 (4)下压气缸 (4)压头装置 (5)热压头 (6)热电偶 (6)热电偶接口 (6)工作台 (7)水平调节 (7)视图系统 (8)变压器 (8)硅胶带装置 (8)真空发生器 (9)主供气单元 (10)输入按钮 (10)控制面板 (11)第 2 章 : 安装要求 (14)气源 (14)环境 (14)安全 (14)第 3 章 : 产品安装和使用 (15)机器安放位置 (15)包装 (15)开机 (16)用户界面 (16)快速启动 (17)功能键 (18)生产 (19)参数 (19)工程 (26)手动 (30)错误信息 (32)加热错误 (32)SCR卡报警 (32)超温 (32)硅胶报警 (32)密码恢复 (32)操作工序 (33)第 4 章 : 校正 (35)温度校正 (35)触摸屏校正 (37)第 5 章 : 产品维护 (38)热压头 (39)变压器电缆线 (39)真空 (40)夹具 (40)安装 (40)热压头保养 (40)水平调节 (40)电源 (41)气源 (41)保存 (41)安全标示 (41)接地标志 (41)PE标志 (41)有害电压标示 (42)夹伤或割伤标示 (42)高温标示 (42)第 6 章 : 故障排除 (43)第 7 章 : 电图 (46)第 8 章 : 气图 (49)信力科技(私人)有限公司成立于1995年，主要设计和制造一系列标志为品牌的精密热能应用设备，如ACF预贴机，脉冲式热压机，回流焊接机，COG/COF邦定机等。

目前，信力的销售网络已扩展至亚洲区域如台湾，中国，香港，马来西亚和菲律宾等。

协同信力机械，信力科技的目标是通过完善的世界销售网络将符合世界水准的产品和服务准时送交给我们的客户。

遥控器产品说明书【篇一：遥控器使用说明书】遥控器使用说明书一、面板说明1、“”键：机器通电并处于开启状态，按此键，机器进入关闭状态；机器通电并处于关闭状态，按此键，机器进入开启状态。

2、“home”键：表示选择进入系统主页面；3、“menu”键：在sw播放器界面，按此键弹出操作菜单；机器处于安卓系统界面，4、“”键：开启或关闭播放机声音。

5、“”键：按3秒钟，进入鼠标模式，可以通过遥控器方向键来控制鼠标的移动，再次按3秒钟退出鼠标模式。

6、“7、“8、“9、“”键：方向键，可以向上移动光标；在鼠标模式可以向上移动鼠标” 键：方向键，可以向左移动光标；在鼠标模式可以向左移动鼠标” 键：方向键，可以向右移动光标；在鼠标模式可以向右移动鼠标” 键：方向键，可以向下移动光标；在鼠标模式可以向下移动鼠标10、“” 键：此按键无功能11、“vol+” 键：调大播放机音量12、“ok” 键：确定按钮13、“vol-” 键：调小音量14、“tab” 键：切换光标位置15、“” 键：此按键无功能16、“” 键：在sw播放器界面，按此键弹出操作菜单；安卓系统界面，返回上一级界面。

17、“1” 键：输入数字1。

18、“2” 键：输入数字219、“3” 键：输入数字320、“4” 键：输入数字421、“5” 键：输入数字522、“6” 键：输入数字623、“7” 键：输入数字724、“8” 键：输入数字825、“9” 键：输入数字926、“0” 键：输入数字027、“.” 键：输入符号“.”28、“del” 键：删除文件或字符29、“vod” 键：此按键无功能30、“live” 键：此按键无功能31、“pause” 键：此按键无功能32、“play” 键：此按键无功能方向键包含：“”键、“” 键、“” 键和“” 键。

数字键包含：“1”“2”“3”“4”“5”“6”“7”“8”“9”“0”“.”二、常用操作1，如何退出sw播放器按“menu”键，弹出选择菜单，按向下方向键“”到“退出”，按“ok”键退出sw播放器。

EndoFectin TM -Max 转染试剂高效转染核酸到哺乳动物细胞产品编号：EF003 / EF004 / EF003T 包装规格：1 mL / 3 mL / 100 μL储存条件：4℃~8℃密闭保存，可保持稳定至少12个月，常温运输。

产品概述EndoFectin TM -Max 转染试剂是以脂质体转染为原理的转染试剂，它能与核酸形成复合物，并使该复合物进入哺乳动物细胞。

EndoFectin TM -Max 转染试剂广泛适用于常见细胞系，如HEK-293、HEK293T 、CHO-K1、Hep G2、Hela 、MCF-7、NIH/3T3和A549等。

即使在有血清存在的情况下，该试剂仍能高效将核酸导入细胞。

GeneCopoeia 公司提供的EndoFectin TM -Max 转染试剂具有如下优点： y 转染效率更优良 y 细胞毒性低y 适用于多种细胞系的转染操作，操作简便y 与含血清的培养基相兼容，转染前不需去除细胞培养液或血清，转染后不需清洗细胞质量控制每批次EndoFectin TM -Max 转染试剂均经过转染测试。

将eGFP 表达质粒（GeneCopoeia Cat.No. EX-EGFP-M02）用EndoFectin TM -Max 转染试剂转入亚融合状态的HEK-293细胞，转染24小时后，超过95%的细胞表达eGFP 。

注意事项使用高质量的质粒：请务必使用高质量的转染级无内毒素质粒。

可通过260 nm 光吸收测定DNA 浓度，并以260 nm / 280 nm 比值确定DNA 纯度（比值应在1.8~2.0的范围内）。

如有可能，请通过琼脂糖凝胶电泳检测质粒的完整性。

保证细胞状态：请使用适当保存和经常传代的健康细胞，并确保培养基无细菌、真菌或支原体污染。

如果细胞是近期复苏的液氮冻存细胞，请在转染前至少传代两次。

实验材料y EndoFectin TM -Max 转染试剂、含目的基因的DNA 质粒y 无蛋白细胞培养液（如Opti-MEM I TM ，来自Life Technologies . 货号：31985-088） y 培养至70~80%汇合度的目的细胞条件摸索在进行正式转染前，推荐以EndoFectin TM -Max 转染试剂摸索目的细胞的最佳转染条件。

Thermo Scientific TM TDE 系列超低温低温箱安装和操作332851H62 • 修订版 A • 2022 年 8 月重要事项请阅读本安装和操作手册。

如果不遵守本手册中的说明，可能会导致设备损坏、操作人员受伤以及设备性能下降。

注意所有内部调整和维护必须由符合所需资格的服务人员进⾏。

本手册中的材料仅供参考。

手册内容及其所包含的产品信息如有变更，恕不另⾏通知。

Thermo Fisher Scientific 对本手册不作任何声明或保证。

对于任何由于使用此手册而引起的直接或间接损坏，Thermo 不承担任何责任。

© 2022 Thermo Fisher Scientific Inc。

保留所有权利。

目录型号 (1)安全考量因素 (2)符号术语 (3)拆包 (5)附件 (5)常规建议 (6)温度监测系统 (6)日常使用 (6)首次加载 (6)格栅门打开/关闭 (6)操作标准 (7)电气规格 (7)安装 (8)位置 (8)保护性导体电流 (8)布线 (8)调平 (9)刮冰刀 (9)备用系统（选配） (9)门操作 (9)安装远程报警连接器 (9)适用范围 (9)操作 (10)初始启动 (10)操作概述 (10)显示画面 (10)设置 (11)关机 (12)刮冰器说明 (13)用途 (13)备用系统（选配） (14)CO2 和 LN2 的预防措施 (14)安装 (14)启动 (15)操作 (15)温度记录仪（选配） (16)设置和操作 (16)更换图表纸 (16)校准调节 (16)维护 (17)清洁冷凝器 (17)清洁冷凝器过滤网 (17)门封维护 (17)低温箱除霜 (17)电池维护 (17)维护计划 (18)故障排除指南 (19)错误代码 (23)保修 (24)保修（国际） (25)附录A：报警汇总 (26)附录B：Modbus ASCII 参数表 (28)临终关怀 (36)WEEE 合规性 (37)联系信息 (39)型号表格 1. 适用型号TDECxxx86F*063/252/396A/D/V/L/K通用型Thermo Fisher Scientific ULT Freezers 型号 | 12 | 安全考量因素Thermo Fisher Scientific ULT Freezers安全考量因素本手册将采用以下符号规则：以下是可能在本产品上使用到的符号和安全警告：（⻅第 5 和第 6 ⻚的列表）以下是本产品的重要安全事项：此符号单独使用时指示会降低人员受伤⻛险或装置性能不良⻛险的重要操作说明。

U ser manualConfiguration softwareLCC-100softwarefor Monostats - Class 50 and 100CONSTRUCTEURunits - ranges - relays - set points time-delay - outputs - channelsAir velocityPressure Humidity Air flow Temperaturew e NI - Minimum System RequirementsII - Connecting the transmitterIII - Displaying and modifying the configurationIV - Saving a configurationV - Opening a configurationVI -Main menuI 1 - Minimum recommended configuration .....................................................................p 2I 2 - Installing the software ...........................................................................................p 2I 3 - Uninstalling the software ........................................................................................p 2I 4 - Launching the software ..........................................................................................p 2II 1 - Warnings .............................................................................................................p 2II 2 - ........................................................................................................p 3Connections III 1 - Principle .............................................................................................................p 3-4III 2 - Tab "Generalities"................................................................................................p 4III 3 - Tab "Channels"....................................................................................................p 4-7III 3a - Settings DIP switches .................................................................................p 4-5III 3b - Measurements ..........................................................................................p 5III 3c - Channels ..................................................................................................p 5Name of the channels .............................................................................p 5Measuring ranges - lower and upper ranges ..................................................p 5-6Units of measurement ............................................................................p 6-7Offset ...............................................................................................p 7III 4 - Tab "Alarm” (monostats)......................................................................................p 7-8III 4a - activate the relay ........................................................................................p 8III 4b - set the time-delay of the relay .......................................................................p 8III 4c - set the set points ........................................................................................p 8III 4d - set the alarm type (rising / falling edge)...........................................................p 8IV 1 - Principle .............................................................................................................p 9IV 2 - Saving a configuration .........................................................................................p 9V 1 - Principle ..............................................................................................................p 9V 2 - Opening a configuration ........................................................................................p 10-11V 3 - Transfer a configuration ........................................................................................p 11V 4 - Delete a configuration ...........................................................................................p 11"Commands" menu .....................................................................................................p 11"Parameters" menu .....................................................................................................p 11"Help" menu ...............................................................................................................p 11S ummaryI - Minimum system requirementsI 1 - Minimum system requirements:Pentium II 300 MHz - 32 MB RAM CD-Rom drive Windows 98-NT4-XP 20 MB free on hard disk Minimum resolution 800x600 (1024x 768 recommended)lllll l l Serial communication port (RS232)Internet Explorer 6.0 or betterII - Connecting the transmitterI 2 - Installing the software :Insert the CD into the CD-ROM driver. The Kimo welcome window appears automatically.If the installation does not automatically begin, click Start and click Run. In the Run dialog box, type : d:\detup (d is the letter of your CD-Rom drive) then click OK.I 3 - Uninstalling the software :To uninstall the "LCC100", it is required to have the rights (under NT) and to use the Windows tool:Go to the menu "Run", "Parameters", “Control panel", then "Install/Uninstall programms ".In the field « Installation/Uninstallation », click on “KIMO LCC100” and follow the instructions.l l I 4- Launching the software:To run the LCC application:Click on the iconfrom your desktopORClick on Start / Programms and LCC 100.llPosition of the DIP switches to access the configurationl l By positioning the DIP switches as shown beside:You may access the configuration via software.The following message will appear when starting the LCC-100 programm:In that case, you will not be able to configure the measuring ranges, the units and the offset of the transmitter.However, you will be able to access to the initial configuration (set with the DIP switch), and to modify the information relative to the alarms (for the PST, HST and TST).To position correctly the DIP switches:- unplug the cable transmitter to the PC- power off the transmitter, set the proper switches as shownbeside and re-power the transmitter.If the DIP switches are wrongly positioned:II 1 - Warnings:III - Displaying and modifying the configurationIII 1 - Principle :Using the function “Read configuration” you may access the configuration of the transmitter.Then, you can read, modify and save the configuration of the transmitter.A new configuration can be transferred to the transmitter and/or saved in a database (for a later use). Some parameters can be modified from the software, others can only be read.II 2 - Connections:To read and modify the configuration of a transmitter, please follow the instructions as below:ll lStep 1 : power up the transmitter (see drawing)Step 2 : please wait a few seconds (time required for the transmitter to initialize).Step 3 : connect the transmitter to the PC with the RS 232 cable.Rs232 cableTopArrow of the RS 232cable’s connectorconnection to transmitterconnection to PCBottomYou can connect the transmitter to the PC before or after the launch of the software.If you make the connection BEFORE launching the sof tware, themessage shown beside will appear when you start the LCC-100.Please click on “YES” and you will directly access the configuration parameters of the transmitter (see page 4).If you first launch the software (before the connection of the transmitter),you access to the 2 main menus of the LCC-100:- Read configuration.- Open configuration from databasel l When you open the software LCC-100, 2 buttons appear:- Read configuration.- Open configuration from databaseTo access to the configuration parameters, you can - click on “Read configuration” or- go in the menu “Commands” and then, click on “Display the configuration” (see page11).Click on nextIII 2 - Tab"Generalities” :The tab "Generalities” shows all relative info of the transmitter, regardless of the configuration.The description of the transmitter:It’s the part number of the transmitter which is connected (ex : TST-B, CP101, CTV100, etc).The version of the software.The analog output:It depends on the transmitter: 4-20 mA or 0-10 V.l l lThe window shown beside appears:This window has 2 or 3 tabs (according to the transmitter connected) and a function button.. Via the unction button, you can trans er the configuration to the transmitter.llThe tabs "Generalities", "Channels" and "Alarm” Click on the tab required to display the information.shows all relative info regarding the configuration of the transmitter. tab "Alarm"This tab only concerns the manostats.It is displayed only when a PST, TST or HST is connected.123III 3 - Tab"Channels" :in f ormation o f switches, the measuring ranges, the units of measurement, the offset.You can see the position of the DIP switches on the electronic board of the transmitter.For the transmitters with 2 DIP switches, the software will display both.With an air velocity and temperature transmitter type CTV 100 : the software displays both air velocity and temperature channels. The measuring ranges displayed are relative to the initial configuration of the transmitter.They are configurable using the lower and upper ranges input boxes (displayed inwhite). Via the arrows located on the right of the zone, you can modify the ranges, or you can enter the value via the keypad.When a value is modified, the writing bar becomes red. It means that the configuration displayed is not the same as the one of the transmitter. When all the modifications required are done (measuring ranges units,offset and/or alarms) click on the writing bar totransfer the new data to the transmitter.Click on the “measure” button to display in real-time the measurements made by the transmitter (see window shown beside). According to the transmitter used, the window displays one or two values, and also displays the status of the relay (for the monostats).Number of valuesPart number of the tranmsitterdisplayedPST, CP50, CP100, TST , HM50, TM100, TG1001 value 2 valuesCTV100, HST, TH100The channels are relative to the type of measurements. You may access to the parameters of measuring ranges (lower and upper ranges), the units of measurements and the offset (only for the models HM50, TH100 and the humidistats HST).depends on the transmitter connected.The description of the channels Part numberType of channel displayedof the t ransmitter channel ‘pressure’channel ‘air v elocity’ channel ‘temperature’ channel ‘humidity’PST CP50CP100TST TM100TG100HST HM50TH100CTV100[[[[[[[[[[[[[The transmitters with conf igurable units and measuring ranges (CP50, CP100, CTV100, TG100 and TH100), work as below:The zone “measuring ranges” and “units” are activated (window 1). Click on the arrow located on the right of the zone “units”, in orderto display the list of all units available. Please select the unit required (click on it). The values of low and upper ranges are changed into “0” and are displayed in red (window 2).Then, you can enter the new values of themeasuring ranges (with low and high values).Click on the writing bar (red), when all the modifications required are done.The units of measurement (on the right of the zone “measuring range)”are pre-set according to the transmitter whichis connected. The unit displayed corresponds to the initial configuration of the transmitter.To modifiy the unit of measurement, click on the arrow located on the right of the zone “units”, in order to display all the available units.When the unit is modified, the writing bar becomes red. It means that the configuration displayed is not the same as the one of the transmitter.When all the modi f ications required are done (measuring ranges, units, offset and/or alarms) click on the writing bar totransfer the new data to the transmitter.Measuring range Minim u m d e l t a Maximum delta CP500 to +10 000 Pa 1000 Pa 10000 Pa CP1010 to +1000 Pa 100 Pa 1000 Pa CP1020 to +1000 mmH 2O100 mmH 2O 1000 mmH 2O CP1030 to +500 mbar 50 mbar 500 mbar CP1040 to +2000 mbar 200 mbar 2000 mbar TH100-50 to +400 °C 50 °C 400 °C TM100-50 to +400 °C 50 °C 400 °C TG100-50 to +400 °C 50 °C 400 °C0 to 30 m/s 3 m/s 30 m/sCTV100-50 to +400 °C 50 °C 400 °C‘over’ range exclamation pointsTo define the measuring ranges, please follow thechart below. If not (for example: going beyond the max. measuring range), then 2 exclamation points will appear on the screen. Then, you will have to enter new and correct values.unit and value convertedThe offset located on the right of the window “channel” enables you to modify the measurement made by thetransmitter.Enter the positive or negative value in the field “Offset”.The writing bar becomes red. Click on it to transfer the configuration to the transmitter.Then the value of the offset is added (or deducted) from the measurement made by the transmitter.ex : a humidity transmitter displays 45 %RH. If you enter ‘10’ in the field “Offset”, and then click on the writing bar, the value measured by thell l Transmitter OFFSET OFFSETpart numberi n humidityin temperatureHST HM50TH100[[[[[III 4 - Tab "Alarm” :On the manostats, you need to configure the relaty output.With the tab “alarm”, you can configure the RCR relays with:Time-delay of relay l l l l Activation of relays Setpoint setting Setting of the type of the alarm (rising/falling edge)Part number Number of alarmsof the transmitter ‘pressure’ channel ‘temperature’ channel “humidity’ channel PST TST HST[[[[PSTCP50CP100TST TM100TG100HST HM50TH100CTV100configurable range configurable units[[[[[[[[[[[[[[[XXXX X[Xnon configurable ranges and/or units configurableranges and/or unitsNota : the humidity is a parameter which cannot be configured, neither for the measuring ranges, nor for the units.To activate the relay , please tick off the box as shown beside.To deactivate the relay, please untick the box as shown beside.The time-delay is a limit which, when being reached and/or exceeded, activates the relay and then the visual red LED alarm.To enter the time period (max. 60 seconds), you can EITHER use the arrows (on the right of the zone), OR enter the value required.ex : time-delay = 10 sec: the alarm will be activated 10 seconds after the setpoint is exceeded'.You can set the setpoint that will activate the alarm (below or above).You can EITHER use the arrows (on the right of the zone) OR enter the value required.ex : setpoint = 50 Pa: the alarm will be activated when the measurement exceeds 50 Pa.You can choose the action:above the setpoint (rising edge)below the setpoint (falling edge).ex : falling action, setpoint 50 Pa, time-delay 0: exceeds 50 Pa.l lIV 2 - Saving a configuration :Please follow carefully the different steps as below:llllUsing the “read configuration” function, read the parameters of the transmitters Make some modifications if required Write the new configuration after having modified In the menu "Commands" click on"Save configuration".IV - Saving a configurationIV 1 - Principle :Using the function “Read configuration", you can record the different configurations into a database.Using the function “Save configuration”, you can save the configurations of the transmitter, and also the the modifications.The window shown beside appears.On the bottom of the window, the field enables you to name each saved configuration.If you do not fill it in, and by default, the date and time of the save will be displayed.Nota : the names o f the con f iguration are preceded by the name of the transmitter which is connected.data field:Click on “Cancel” to cancel the save or click on “OK" to continue.By clicking on "OK", the window shown beside is displayed. Click on "OK" to return to the main menu.V - Opening a configurationV 1 - Principle :Using the function "Open configuration from database", you may access to the database of all the different configurations saved.Then, it is possible to match one configuration with one (or several) transmitter(s). You do not need to make the configuration each time.Via this function, you can:Open a configuration.Transfer a configuration to one or several transmitter(s)Delete a configuration.l l l When launching the LCC-100 software, two buttons are displayed:ll Read configurationOpen configuration from databaeV 2 - Opening a configuration :To open a configuration, click on “Open configuration from database” or go in “Commands” and then, click on “” (see p11). The window shown beside will be displayed.To choose a configuration :Open configuration from database First, select the type of transmitter (Class) that you want to configure (see beside)Then, the parameters relative to the Class chosen are displayed in a second box.Select the parameter required. Then, the configurations saved are displayed in the last box.Then, you can choose the configuration required.l llex : to choose a configuration for a transmitter TG 100, select “100” in the box “Class” . Then, select “Temperature” in the box “Parameters”.Then, the list of configurations saved for the TG 100 are displayed in the last box.To open a configuration, click on the name of the configuration required (as explained p10). The name chosen is then displayed.Click on "Open". The windown shown beside asks you to confirm the opening. Then, click on “Yes”.To transfer the configuration to a transmitter which is connected, click on the writing bar or go in “Commands” and click on “Writing of the configuration” (see p11).V 4 - Deleting a configuration :To cancel a configuration, click on the name of the configuration required (as explained p10). The name is then displayed on a blue background. Click on “Cancel”. The window shown beside appears to ask you to confirm: then, click on “Yes”."Help F1" to open the user manual of the software.V 3 - Transfering a configuration:To transfer the configuration parameters to a transmitter , please open the configuration required as explained above.Then, click on the writing bar or go in “Commands” and then, in “Write configuration” (see p11).writing barVI - Main menuR e f . N T - A N G - L C C 100 - 06/04 A .Distributed by :EXPORT DEPART MENTTel : + 33. 1. 60. 06. 69. 25 - Fax : + 33. 1. 60. 06. 69. 29site :e-mail :www.kimo.fr**************。

-100桌面型数字电话录音机使用手册数字话机&IP话机&无线固话录音技声语音软件（上海）有限公司（C）2015Eletech Enterprise Co.,LtdEletech Voice Systems Inc.All Rights Reserved目录一、面板及外观介绍 (1)（一）俯视图 (1)（二）后视图 (3)（三）侧视图 (4)（四）安装图 (5)二、按键定义 (6)（一）播放模式 (6)（二）录音模式 (7)（三）空闲模式 (7)（四）设置模式 (7)三、使用操作 (10)（一）录音模式 (10)（二）播放模式 (12)（三）设备设置 (14)（四）格式化SD存储卡 (15)（五）删除文件 (16)四、CallPad技术规格 (17)一、面板及外观介绍（一）俯视图1.麦克风孔（内置麦克风用于现场谈话录音）2.LCD 显示屏3.扬声器4.头戴式耳麦切换麦克风静音模式5.②减小输出音量；修改数值（设置模式）6.④II 播放当前录音或暂停当前播放7.退出当前设置项/停止播放/停止录音8.录音按钮(LED 灯):LED 熄灭=空闲模式;绿色=待机模式;红色=录音中自动模式（VOX ）：-LED 灯熄灭状态，设备不会录音。

-按此按键，LED 灯变成绿色，进入待机模式-LED 灯绿色状态下，拿起话机手柄将开始录音(LED 灯变成红色)1.内置麦克风2.LCD 显示屏3.扬声器4.头戴式耳麦（Headset MIC ）静音键5.“向下”或减小音量6.播放及暂停键7.停止键8.录音键（带LED 双色灯）9.手柄/头戴式耳麦切换Handset /Headset 10.话机/内置麦克风切换Phone/MIC11.向左或播放快退12.向上或增加音量13.向右或播放快进14.删除记录键15.重要标记键16.解锁键Unlock key17.手柄及耳麦兼容设置滑档手动模式：Manual-LED灯熄灭状态，设备不会录音。