内参
1.RNU6
Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA NR_004394.1 ORIGIN
1 gtgctcgcttcggcagcacatatactaaaattggaacgatacagagaagattagcatggc
61 ccctgcgcaaggatgacacgcaaattcgtgaagcgttccatatttt
RNU6-F
CTCGCTTCGGCAGCACA
RNU6-R
AACGCTTCACGAATTTGCGT
2.5S
Homo sapiens RNA, 5S ribosomal 2 (RNA5S2), ribosomal RNA NR_023364.1 ORIGIN
1 gtctacggccataccaccctgaacgcgcccgatctcgtctgatctcggaagctaagcagg
61 gtcgggcctggttagtacttggatgggagaccgcctgggaataccgggtgctgtaggctt
121 t
5S-F
TCTCGTCTGATCTCGGAAGCTA
5S-R
GCGGTCTCCCATCCAAGTA
MiRNA
方案1
Universal Reverse primer TGGTGTCGTGGAGTCG
方案2
Universal Reverse primer GTGCAGGGTCCGAGG
1.hsa-miR-122
序列：uggagugugacaaugguguuug 长度：22
2.Hsa-miR-16
序列：uagcagcacguaaauauuggcg 长度：22
3.hsa-mir-93
序列：caaagugcuguucgugcagguag 长度：23
Primer Result:
miRNA miRNA sequence (5'-3') Prime
r
Primer Sequence (5'-3')
Len
gth
of
prim
er
Tm
of
prim
er
GC content
of primer
hsa-mir-122 TGGAGTGTGACAATGG
TGTTTG
RT
prime
r
CTCAACTGGTGTCGTGGAGTCGGCAATTCAG
TTGAGCAAACACC
44
0.52272727
2727
Forw
ard
prime
r
ACACTCCAGCTGGGTGGAGTGTGACAATG 29 90 0.55
hsa-mir-16 TAGCAGCACGTAAATA
TTGGCG
RT
prime
r
CTCAACTGGTGTCGTGGAGTCGGCAATTCAG
TTGAGCGCCAATA
44
0.52272727
2727
Forw
ard
prime
r
ACACTCCAGCTGGGTAGCAGCACGTAAAT 29 88 0.52
hsa-mir-93 CAAAGTGCTGTTCGTG
CAGGTAG
RT
prime
r
CTCAACTGGTGTCGTGGAGTCGGCAATTCAG
TTGAGCTACCTGC
44
0.54545454
5455
Forw
ard
prime
r
ACACTCCAGCTGGGCAAAGTGCTGTTCGTG 30 94 0.57
Primer Result:
miRNA miRNA sequence
(5'-3')
Prime
r
Primer Sequence (5'-3')
Leng
th
of
prim
er
Tm
of
prim
er
GC
conte
nt of
prime
r
hsa-mir -122 TGGAGTGTGACAATGG
TGTTTG
RT
prime
r
GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACT
GGATACGACCAAACA
50 0.54
Forwa
rd
prime
r
GCGGCGGTGGAGTGTGACAATG 22 72 0.64
hsa-mir -16 TAGCAGCACGTAAATA
TTGGCG
RT
prime
r
GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACT
GGATACGACCGCCAA
50 0.58
Forwa
rd
prime
r
GCGGCGGTAGCAGCACGTAAAT 22 70 0.59
Forwa
rd
prime
r
GCGGCGGCAAAGTGCTGTTCGTG 23 76 0.65
