悬浮培养类似微生物的深层培养。由于 是悬浮生长，细胞密度一般都较高，培 养的效率也高、操作也容易。
这种方法有多方面的优越性，易于大规 模生产，便于过程的控制。
可惜能在悬浮状态下生长的细胞种类有 限，且不太方便观察。
3.2 动物细胞培养 的基本技术
3.2.1 体外培养的条件
3.2.1.1 与微生物细胞培养相比动物 细胞培养的特殊性
Secondary culture－Cells taken from a primary culture and passed or divided in vitro. These cells have a limited number of divisions or passages.
tissue culture - the maintenance of a tissue or fragment in a way that may allow differentiation and preservation of the architecture and/or function
细胞贴壁延展过程
Contact inhibition When cells contact each other, they cease their growth. Cells arrest in G0 phase of the cell cycle. Transformed cells will continue to proliferate and pile up each other.
死亡。
3.1.2 体外培养
原代培养（亦称初代培养） 传代培养（亦称继代培养）。
Primary Culture
outgrowth and migration of cells
