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谷氨酰胺对断奶仔猪血清、肌肉指标和肌肉热休克蛋白表达的影响陈静;王丽;王彤;刘显军;李海东;程奇【摘要】A total of 32 crossbred (Landrace×Yorkshire) pigs were used to determine the effect of glutamine on serum indexes, muscle indexes and expression of heat shock protein (HSP 70) of muscle. The piglets were divided into 2 treatments. The control group was fed with basal diet, while the glutamine group was fed with diet added with 1.2% glutamine (Gln). The pigs fed with diet supplemented with glutamine showed an increase tendency (p﹥0.05) concentrations of total protein, albumin protein, alanine aminotransferase and decrease (p﹥0.05) of protein coefficient compared with the control. Glutamine supplementation increased (p﹤0.05) concentrations of muscle glutathione (GSH) and glutathione s-transferase (GST) compared with the control. Glutamine supplementation decreased (p ﹤0.05) concentrations of muscle γ-L-glutamyl transpeptidase (γ-GT) and malondialdehyde (MDA) compared with the control. There were no differences (p﹥0.05) in muscle superoxide dismutase (SOD) between pigs fed with the control diet and pigs fed with the Gln diets. The expression of HSP70 in muscle was greater in piglets supplemented with Gln than in control piglets (p﹤0.05). The results indicate that Gln supplementation could improve the synthesis of protein and muscle antioxidant properties and increase muscle HSP70 expression.%为研究谷氨酰胺对断奶仔猪血清、肌肉生化指标和和肌肉热休克蛋白表达的影响，选用(28±1)d 断奶的长白×大约克夏仔猪32头，随机分为2组，对照组饲喂基础日粮，谷氨酰胺组饲喂添加1.2%谷氨酰胺的基础日粮，试验期28d。
谷氨酰胺对脂多糖应激仔猪生长性能及血清生化指标的影响田军权;何流琴;黄牛;李欢;崔志杰;李思;姚康【摘要】The objective of the study was to evaluate the effects of dietary glutamine ( Gln) supplementation on growth performance in the different stages, and the effects of Gln on growth performance and serum biochemi?cal parameters of weaned piglets after intestinal injury induced by lipopolysaccharide ( LPS ) . Twenty?four cross?bred(Duroc×Landrace×Yorkshire) piglets weaned at 28 days of age were randomly assigned into 3 groups with 8 replicates per group and 1 pig per replicate. Pigs in the control group and LPS group were fed the basal diet, and the others in the Gln+LPS group were fed the basal diet supplemented with 1% exogenous Gln. On day 22, 25, 28 and 30 of the trial, piglets in the LPS and Gln+LPS groups were administered intraperito?neally with 100μg/kg BW LPS, whereas pigs in the control group were injected intraperitoneally with the same volume of sterile saline. The experiment lasted for 30 days. The results showed as follows: 1 ) before LPS challenge ( day 1 to 21 of the trial) , compared with the control group, the average daily feed intake ( AD?FI) and average daily gain ( ADG) of weaned piglets in Gln+LPS group were significantly increased on day 1 to 7 of the trial ( P<0.05) , the ADFI of weaned piglets in Gln+LPS group was significantly increased on day 8 to 14 and 1 to 21 of the trial (P<0.05). 2) After LPS challenge (day 22 to 30 of the trial), the ADFI, ADG and body weight on day 30 of weaned piglets in control group were significantly higher thanthose in LPS and Gln+LPS groups ( P<0.05) , the ADFI, ADG and body weight on day 30 of weaned piglets in Gln+LPS group were higher than those in LPS group ( P>0.05) . 3) The small intestine length of weaned piglets in LPS group was significantly lower than that in control andGln+LPS groups ( P<0.05) , but there was no difference between the control group and Gln+LPS group ( P>0.05) . 4) Compared with the control group, the high den?sity lipoprotein cholesterol content and alkaline phosphatase activity in serum of weaned piglets in Gln+LPS group were significantly decreased (P<0.05), but there was no difference between the Gln+LPS group and LPS group ( P>0.05); the serum immunoglobulin M content of weaned piglets in Gln+LPS and LPS groups was significantly increased ( P<0.05) . The results indicate that dietary supplemented with 1% Gln can improve the growth performance of piglets after weaning 1 to 7 day, and then it is not obvious. Dietary supplemented with 1% Gln can regulate the serum biochemical parameters, improve the growth performance and small intes?tine length of piglets, thereby alleviate weaning stress.%本试验旨在研究饲粮中添加谷氨酰胺(Gln)对断奶仔猪不同阶段生长性能的影响,以及其对脂多糖(LPS)诱导肠道损伤后断奶仔猪血清生化指标和生长性能的影响.选用24头28日龄健康的"杜×长×大"三元杂交断奶仔猪,随机分为3组,每组8个重复,每个重复1头猪.对照组和LPS组饲喂基础饲粮,Gln+LPS组饲喂添加了1%的外源性Gln的基础饲粮;在试验第22、25、28、30天,LPS组和Gln+LPS组腹腔注射100μg/kg BW LPS,对照组则注射相同剂量的生理盐水.试验期30 d.结果表明:1)LPS处理前(试验第1~21天),与对照组相比,Gln+LPS组显著提高了试验第1~7天断奶仔猪的平均日采食量(ADFI)和平均日增重(ADG)(P<0.05),显著提高了试验第8~14天和第1~21天断奶仔猪的ADFI(P<0.05).2)LPS处理后(试验第22~30天),对照组断奶仔猪的ADFI、ADG、第30天体重均显著高于LPS组和Gln+LPS组(P<0.05),Gln+LPS组断奶仔猪的ADFI、ADG、第30天体重均高于LPS组(P>0.05).3)LPS组断奶仔猪的小肠长度显著低于对照组和Gln+LPS组(P<0.05),而对照组和Gln+LPS组之间无显著差异(P>0.05).4)与对照组相比,Gln+LPS组断奶仔猪的血清高密度脂蛋白胆固醇(HDLC)含量和碱性磷酸酶(ALP)活性显著降低(P<0.05),而Gln+LPS组与LPS组之间无显著差异(P>0.05);Gln+LPS组和LPS组断奶仔猪的血清免疫球蛋白M(IgM)含量显著提高(P<0.05).由此可见,饲粮中添加1%的Gln能够显著提高仔猪断奶后第1~7天的生长性能,之后效果不明显.饲粮中添加1%的Gln能够调节应激仔猪的血清生化指标,改善其生长性能和小肠长度,从而缓解仔猪断奶应激.【期刊名称】《动物营养学报》【年(卷),期】2017(029)005【总页数】8页(P1670-1677)【关键词】谷氨酰胺;仔猪;脂多糖;生长性能;血清生化指标【作者】田军权;何流琴;黄牛;李欢;崔志杰;李思;姚康【作者单位】中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南省畜禽健康养殖工程技术中心,农业部中南动物营养与饲料科学观测实验站,长沙 410125;中国科学院大学,北京 100049;中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南省畜禽健康养殖工程技术中心,农业部中南动物营养与饲料科学观测实验站,长沙 410125;中国科学院大学,北京 100049;湖南农业大学动物科学技术学院,长沙 410128;湖南农业大学动物科学技术学院,长沙 410128;湘潭大学,湘潭 411105;湖南农业大学动物科学技术学院,长沙 410128;中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南省畜禽健康养殖工程技术中心,农业部中南动物营养与饲料科学观测实验站,长沙 410125【正文语种】中文【中图分类】S828哺乳仔猪因自身合成的谷氨酰胺(Gln)不能满足机体需求，且消化道发育不完善，外加早期断奶应激的影响，导致了仔猪获取外源性Gln的能力较弱[1]，因此，断奶仔猪饲粮中及时补充外源性Gln显得尤为重要。
谷氨酰胺对断奶仔猪肠黏膜更新的影响及其机制李雪;王小城;熊霞;印遇龙【摘要】Glutamine ( Gln) plays a key role in production of piglets. As an essential nutrient and energy sub⁃stance of the proliferation of intestinal epithelial cells, Gln can maintain the normal structure and function of the intestinal tract, and promote regeneration of intestinal tract mucosal cells. It is also important to reduce the stress of weanling stress and improve the production performance of piglets. The present paper focused on the mechanism and effects of Gln on intestinal mucosal regeneration of weaned piglets.%谷氨酰胺（ Gln）在仔猪生产中具有重要的意义，作为肠上皮细胞增殖的必需营养物和能源物质，Gln能够维持肠道结构和功能的完整，促进肠道黏膜细胞的更新，缓解仔猪的断奶应激，提高仔猪的生产性能。
本文就Gln对断奶仔猪肠黏膜更新的影响及其机制进行综述。
【期刊名称】《动物营养学报》【年(卷),期】2016(028)012【总页数】6页(P9-14)【关键词】断奶仔猪;谷氨酰胺;肠道营养;黏膜更新;影响;机制【作者】李雪;王小城;熊霞;印遇龙【作者单位】湖南农业大学动物科学技术学院，长沙 410128; 中国科学院亚热带农业生态研究所，中国科学院亚热带农业生态过程重点实验室，长沙 410125;中国科学院亚热带农业生态研究所，中国科学院亚热带农业生态过程重点实验室，长沙 410125;中国科学院亚热带农业生态研究所，中国科学院亚热带农业生态过程重点实验室，长沙 410125;湖南农业大学动物科学技术学院，长沙 410128【正文语种】中文【中图分类】S852.2;S828为了增加年产仔窝数，避免母猪和仔猪疾病交叉感染，增加经济效益，生产上通常对仔猪实行早期断奶(3～4周龄)。
谷氨酰胺对断奶仔猪的生理作用李琳胡章明(湖南省益阳市赫山区动物卫生监督所,湖南益阳413043)摘要:仔猪断奶时,由于环境因素的改变和由适应的母乳转换成固体饲料的过程,容易导致仔猪断奶应激,断奶应激造成仔猪腹泻、免疫力降低,死亡率增加;谷氨酰胺是动物肠粘膜主要的能量物质,对动物肠黏膜具有保护和修复作用。
本文就谷氨酰胺对断奶仔猪的生理作用进行综述。
关键词:断奶仔猪谷氨酰胺肠道谷氨酰胺(Gln)是泌乳21天的母猪乳汁中含量最丰富的自由氨基酸(1.93mmol/l)。
机体谷氨酰胺量占总游离氨基酸一半以上,因此谷氨酰胺的变化直接影响机体总氨基酸的变化。
由于机体对谷氨酰胺的生物合成和降解能力很强,不需经口服摄入就足以满足机体的需要,40年代Rose.WC 确定人体内8种必需氨基酸以后,人们一直认为谷氨酰胺是营养性非必需氨基酸。
加上谷氨酰胺的热不稳定性和在水溶液或酸性环境中可自行环化生成焦谷氨酸或分解成谷氨酸(Glu),故Gln一直被忽略。
直到50年代,Eagle等发现谷氨酰胺是培养细胞分化增殖所必需的营养物质,才提出谷氨酰胺可能具有重要而独特的代谢的观点。
近年来又有些试验表明:Gln在应激条件下(如断奶,感染,受伤等)可能是“条件必需氨基酸”。
Gln是“条件必需氨基酸”,在营养与代谢方面有多种重要的功能。
1断奶对仔猪生理影响断奶导致仔猪小肠在形态和功能上的异常,包括固有膜厚度的增加,肠绒毛高度的降低,二糖酶活性降低及吸收功能障碍,并能导致碳水化合物代谢、氨基酸代谢、脂类代谢的异常。
因此仔猪断奶常出现腹泻、釆食量下降、生长缓慢和免疫力降低等早期断奶综合症。
断奶时仔猪肠绒毛脱落,面膜水肿,固有膜中有大量嗜酸性白细胞浸润,肠道跨膜电阻减小,通透性增加,肠致密性蛋白降低。
仔猪断奶后空肠萎缩的原因可能有:(1)断奶切断了乳汁Gln来源;(2)断奶后一段时间内仔猪采食量低,由日粮摄入的Gln少,导致体内Gln的不足。
谷氨酰胺二肽对断奶仔猪生长、免疫、抗氧化力和小肠黏膜形态的影响席鹏彬;林映才;蒋宗勇;郑春田;朱选【期刊名称】《饲料博览：技术版》【年(卷),期】2007()9【摘要】文章旨在研究谷氨酰胺二肽（Gln二肽）对断奶仔猪生长、免疫、抗氧化力和小肠黏膜形态的影响，探讨Gln二肽缓减仔猪断奶应激的机理。
选用96头平均体重（6．23±0、13）kg（杜×大×长）三元杂交仔猪，按完全区组设计分为4组，每组4个重复，每个重复6头（公母各1／2），各处理仔猪分别饲喂基础饲粮、基础饲粮添加0．125％Gln二肽、基础饲粮添加0、25％Gln二肽和2％血浆蛋白粉的试验饲粮。
【总页数】2页(P61-62)【关键词】谷氨酰胺二肽;断奶仔猪;生长;免疫;抗氧化力;小肠黏膜形态【作者】席鹏彬;林映才;蒋宗勇;郑春田;朱选【作者单位】广东省农业科学院畜牧研究所,广东省动物育种与营养公共实验室,广州510640【正文语种】中文【中图分类】S858.28【相关文献】1.丙氨酰谷氨酰胺二肽对仔兔生长性能、小肠黏膜形态及抗氧化性能的影响 [J], 杨杰;翟频;白文霞2.丙氨酰-谷氨酰胺对断奶仔猪小肠黏膜固有层免疫球蛋白 A浆细胞数量、分泌型免疫球蛋白 A及黏膜中白细胞介素含量的影响 [J], 叶亚玲;王自蕊;游金明;邓宸玺;辛向荣3.断奶仔猪小肠黏膜脂肪酸结合蛋白和二肽转运载体1mRNA表达发育性变化及谷氨酰胺对其的影响 [J], 周琳;曹广添;张帅;杨彩梅;陈安国;洪奇华4.谷氨酰胺二肽对断奶仔猪生长、免疫、抗氧化力和小肠粘膜形态的影响 [J], 席鹏彬;林映才;蒋宗勇;郑春田;朱选5.谷氨酰胺对早期断奶仔猪小肠黏膜形态和抗氧化性能的影响 [J], 边连全;陈静;刘显军因版权原因，仅展示原文概要，查看原文内容请购买。
