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ECTE333-Part-A-Laboratory

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Appendix A Appendix B
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SECTE Laboratory Notes
Introduction
The ECTE333 Laboratory introduces students to the use of a microcontroller board, specifically the Atmel AVR STK500 Board. These experiments have been designed with the aim of familiarising the student with the tools available to program the STK500 board, and to allow the student to use the information they know about the microcontroller to solve various problems. The STK500 board provides access to all components of the Atmel AVR microcontroller. Programs are mainly written in Assembler Language and are compiled and downloaded using the AVR Studio software. Programs can also be written in the C programming language and compiled using the WinAVR software. Students can simulate almost every aspect of the AVR microcontroller on their home PCs by installing the AVR Studio software and using the emulation tools. The programs developed by students are downloaded to the STK500 board via an RS232 link for execution, so for hardware practise, students will require a serial port. This is not needed, as only simulation is required in the home experiments.
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SECTE Laboratory Notes
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SECTE Laboratory Notes
limited time for the laboratory sessions (1 hour to mark ~40 demonstrations), so it is important that time is not wasted asking questions that could have been answered prior to entering the lab. Students must also submit two lab reports throughout the year – one report at the end of each session. See the Subject Information Sheet for details of submission. A title page must be attached to all reports clearly showing the student's name, ID number, subject code and name, and laboratory class. Laboratory cover sheets are available from the School’s main office and online from .au/subjects/cover/. These reports are to be concise summaries of the work performed and should be no more than two pages per experiment in an equivalent of 12 point times font. The report should contain program listings as appendices if relevant. Reports will be marked out of 10. Reports should be handed in as per the deadlines given in the Subject Information Sheet. Late penalties are also described in the Subject Information Sheet. There will be a Practical Test in the University Exam Period. This test will consist of writing a simple program in 55 minutes from scratch. This means the task is to be completed without using any listings of programs or files on any removable storage media, local hard disk or in network drives. A properly formatted laboratory notebook may be brought into and used in the test.
Table of Contents
Introduction Assessment Resources Experiments Experiment 1 Experiment 2 Experiment 3 Experiment 4 Experiment 5 Experiment 6 ……………………………………………… …………………………………………….... …………………………………………….... …………………………………………….... …………………………………………….... …………………………………………….... 8 13 16 18 21 23 …………………………………………………….. …………………………………………………….. …………………………………………………….. 4 4 5
Laboratory Structure
There will be six experiments to be completed in the Autumn Session. A further six experiments will be completed in the Spring Session (see Spring Lab Notes). Across the entire year, the laboratory components comprise 40% of the total assessment for ECTE333, as outlined on the subject information sheet. This is broken down into 20% assessment in Autumn and 20% assessment in Spring. The individual components of the Autumn 20% are as follows: 5% Laboratory Logbooks marked during laboratories 5% Laboratory Report (Due Friday Week 13, 4pm) 10% Practical Exam (University Exam Period) Preparation and conduction of the Home Experiment for the laboratory is mandatory. Before the start of each laboratory session, the students must undertake a WebCT based quiz consisting of approximately 10 questions. Severe penalties will be awarded to students who do not complete this quiz before entering the room (see Subject Information Sheet for details). Proper preparation can be achieved by reading through the home experimental and making notes in your laboratory book of what is observed. Experiments should be completed before entering the laboratory. There is

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3-氯酪氨酸(3-CT)检测

3-氯酪氨酸(3-CT)检测

3-氯酪氨酸（3-CT）检测
3-氯酪氨酸（3-Chlorotyrosine, 3-CT），可用高效液相色谱法检测人血浆中
3-氯酪氨酸的浓度，该方法所需样本量低、预处理步骤简便，适用于临床进行
氧化应激、治疗、预后、诊断等研究工作。

迪信泰检测平台采用高效液相色谱(HPLC)和液质联用(LC-MS)法，可高效、精准的检测3-氯酪氨酸的含量变化。

此外，我们还提供其他氨基酸及其代谢物检测
服务，以满足您的不同需求。

HPLC和LC-MS测定3-氯酪氨酸样本要求：
1. 请确保样本量大于0.2g或者0.2mL。

周期：2~3周
项目结束后迪信泰检测平台将会提供详细中英文双语技术报告，报
告包括：
1. 实验步骤（中英文）
2. 相关质谱参数（中英文）
3. 质谱图片
4. 原始数据
5. 3-氯酪氨酸含量信息
迪信泰检测平台可根据需求定制其他物质测定方案，具体可免费咨询技术支持。

LC-MS氨基酸测定项目样本报告，点击查看>。

一步法TUNEL细胞凋亡检测试剂盒红色TRITC标记荧光检

一步法TUNEL细胞凋亡检测试剂盒红色TRITC标记荧光检

一步法 TUNEL 细胞凋亡检测试剂盒（红色 TRITC 标记荧光检测法，通用型）说明书修订日期：2018.07.31 Catalog No.：KGA7061 / KGA7062 / KGA7063Storage：-20℃ for 12 months,避光For Research Use Only（科研专用）一、TUNEL 检测原理凯基一步法 TUNEL 细胞凋亡检测试剂盒(TRITC红色荧光标记，通用型)提供一种高灵敏度又快速简便的细胞凋亡检测方法，可检测细胞在凋亡过程中细胞核 DNA 的断裂情况，其原理是红色荧光素（Tetramethylrhodamine，TRITC）标记的 dUTP 在脱氧核糖核苷酸末端转移酶（TdT Enzyme）的作用下，可以连接到凋亡细胞中断裂 DNA 的3′－OH 末端，可用荧光显微镜检测。

由于正常的或正在增殖的细胞几乎没有 DNA 的断裂，因而没有3′-OH 形成，很少能够被标记。

本试剂盒适用于组织样本（石蜡包埋、冰冻和超薄切片）和细胞样本（细胞涂片或爬片）的凋亡原位检测。

对于经过固定和洗涤的细胞或组织，只要经过一步染色反应，洗涤后就可以通过荧光显微镜检测到凋亡细胞。

二、TUNEL 试剂盒组分运输及保存条件：2-8℃低温运输，收到后-20℃避光保存，保质期一年。

实验前准备：实验前按说明书准备好相应试剂与器具，多聚甲醛、二甲苯、乙醇、1×PBS pH 7.4、H2O2、Triton X-100、甲醇、多聚赖氨酸铺载玻片（细胞样本）、盖玻片、染色缸、温盒、量筒等。

三、操作步骤各个样本请用免疫组化笔做好标记，另外加 2 张样本切片分别用于阳性片和阴性片制备并做好标记。

在每步反应或浸洗的间隙时，配制下一步即用的工作液。

注意事项反应液最好根椐计算好的样本数量集中配制，再分别滴加于各样本上，避免因每个样本单独配制而产生的试剂损耗，TdT 酶反应液如需短暂保存时，请置于冰上。

迈瑞铁蛋白检测试剂盒说明书

迈瑞铁蛋白检测试剂盒说明书

Manual ProcedureAutomated procedure on requestTest PrincipleFerric ions (Fe +3) are released from transferring under acidicconditions and reduced to ferrous state (Fe +2) by a strong reducing agent. Ferrous ions then react with Ferrozine to form a coloredcomplex which can be measured photometrically. The intensity of the color produced is proportional to the iron concentration in the sample. Lipemic samples are clarified by the detergent.Transferrin-Fe-complex apotransferrin + Fe 3+Fe 3+ Fe 2+Ferrozine + Fe 2+ colored complexStability and preparation of working reagentBuffer Reagent B-R1: liquid Powder Reagent P-R1: powder. Reagent R2: liquid, ready to useWorking ReagentAdd 10 ml reagent Buffer B-R1 to one vial powder reagent P-R1 andmix gently for 15 minutes before use.Stability: 2 weeks at 20 – 25 °C1 month at2 – 8 °CAll reagents are stable up to expiry date given on label when storedat 2 – 8 °CNote : All reagents should be clear solutions. Don’t use if the reagent is turbid. Specimen Collection and Handling1. Non-hemolyzed serum is the specimen of choice.2. Serum should be separated from blood clot as soon as possible.3. Heparinized plasma could only be used, other anticoagulantsshould not be used.4. Serum iron is reported to be stable for 4 days at 20 – 25°C and7 days at 2 – 8 °C.CalibratorMediCal U Cat. No. 15011Iron STD. Cat. No. 16131Quality controlMeditrol N Cat. No. 15171 Meditrol P Cat. No. 15181Iron (Ferrozine)Colorimetric testPowder liquid reagentsCalculationConc.Iron = ⨯ Conc. Standardμμ g /dlLinearity Up to 1000 μg/dl (179 μmol/L); If result exceeds 1000 μg/dl, repeat test using diluted sample (1+1) with sodium chloride solution (0.9 %) and multiply result by 2.Interference 1. Certain drugs and other substances are known to influencecirculating iron levels. 2. To make tubes, pipettes, etc. iron free, they must be washed with diluted (1+2) hydrochloric or nitric acid followed by several rinsings with iron free deionized or distilled water.Precautions 1. The reagent is toxi c, don’t pipette by mouth, avoid all contacts. 2. Use only disposable plastic containers or iron free tubes andcuvettes. Avoid any contamination by the use of clean laboratory material.Cat. No. 12881 B-R1 1 Buffer 50mlFor 50 testsP-R1 5 powder for 10 mlR2 1 x 10 ml(A S -A SB ) SampleA standardGuanidinium PH=4.5 Ascorbic acidIron ferrozine methodReference rangeReferences1. Garoc A., Clin. Chem. Acta 94, 115 (1979).2. Brivio et coll., La Ricerca Clin. Lab. 18, 523 (1986).3. Young, DS., Effects of Drugs on Clinical Laboratory Tests, fifthedition 2000, AACC Press, Washington, D.C.。

化妆品用化学原料体外3T3中性红摄取光毒性试验方法

化妆品用化学原料体外3T3中性红摄取光毒性试验方法

附件化妆品用化学原料体外3T3中性红摄取光毒性试验方法一、范围本方法规定了化妆品用化学原料体外3T3中性红摄取光毒性试验的范围、规范性引用文件、术语和定义、试验原理、试验材料与试剂、试验步骤、结果判定标准。

本方法推荐适用于评价化妆品用化学原料的潜在光毒性。

二、规范性引用文件下列文件中的条款通过本方法的引用而成为本方法的条款。

注明日期的引用文件，其后所有的修改单（不包括勘误的内容）或修订版均不适用于本方法，但是，鼓励使用单位对修订部分的引用进行研究，并提出意见。

研究是否可使用这些文件的最新版本。

未注明日期的引用文件，其最新版本适用于本方法。

经济合作与发展组织（OECD）guidelines for the testing of chemicals: 3T3 NRU phototoxicity test. NO. 432三、术语和定义下列术语和定义适用于本方法。

（一）光毒性（Phototoxicity）皮肤一次接触化学物质后，继而暴露于长波紫外线照射下所引发的一种皮肤毒性反应。

—1—（二）细胞活性（cell viability）测量某一细胞群总活性的参数（如细胞溶酶体摄取活性染料中性红），其数值取决于测定的终点和试验所用的设计方案，并与细胞总数和/或细胞活力相关。

（三）相对细胞活性（relative cell viability）通过与溶剂（阴性）对照组的相关性来表达的细胞活性，对照组除了未经受试化学物质处理外，整个试验过程与试验组一样（或+Irr或-Irr）。

（四）光刺激因子（photo irritation factor; PIF）受试物分别在无光照(-Irr)和有光照(+Irr，无细胞毒性的紫外光/可见光（UV/vis）照射)条件下获得两组平行有效的细胞毒性浓度(IC50)，通过比较IC50的值得到的因子。

（五）半数抑制浓度（IC50）使细胞活性下降50%的受试化学物质的浓度。

（六）平均光效应（mean photo effect; MPE）受试物分别在无光照(-Irr)和有光照(+Irr，无细胞毒性的UV/vis照射)条件下获得两组浓度反应曲线，通过数学分析导出的数值。