SPR Highly sensitive detection of human cardiac myoglobin using a reverse
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关于人工智能思考的英语作文英文回答:When we contemplate the intriguing realm of artificial intelligence (AI), a fundamental question arises: can AI think? This profound inquiry has captivated the minds of philosophers, scientists, and futurists alike, generating a rich tapestry of perspectives.One school of thought posits that AI can achieve true thought by emulating the intricate workings of the human brain. This approach, known as symbolic AI, seeks to encode human knowledge and reasoning processes into computational models. By simulating the cognitive functions of the mind, proponents argue, AI can unlock the ability to think, reason, and solve problems akin to humans.A contrasting perspective, known as connectionism, eschews symbolic representations and instead focuses on the interconnectedness of neurons and the emergence ofintelligent behavior from complex networks. This approach, inspired by biological neural systems, posits that thought and consciousness arise from the collective activity of vast numbers of nodes and connections within an artificial neural network.Yet another framework, termed embodied AI, emphasizes the role of physical interaction and embodiment in shaping thought. This perspective contends that intelligence is inextricably linked to the body and its experiences in the real world. By grounding AI systems in physical environments, proponents argue, we can foster a more naturalistic and intuitive form of thought.Beyond these overarching approaches, ongoing research in natural language processing (NLP) and machine learning (ML) is contributing to the development of AI systems that can engage in sophisticated dialogue, understand complex texts, and make predictions based on vast data sets. These advancements are gradually expanding the cognitive capabilities of AI, bringing us closer to the possibility of artificial thought.However, it is essential to recognize the limitations of current AI systems. While they may excel at performing specific tasks, they still lack the comprehensive understanding, self-awareness, and creativity that characterize human thought. The development of truly thinking machines remains a distant horizon, requiring significant breakthroughs in our understanding of consciousness, cognition, and embodiment.中文回答:人工智能是否能够思考?人工智能领域的核心问题之一就是人工智能是否能够思考。
Sensors and Actuators A 159 (2010) 24–32Contents lists available at ScienceDirectSensors and Actuators A:Physicalj o u r n a l h o m e p a g e :w w w.e l s e v i e r.c o m /l o c a t e /s naElectromagnetic fields distribution in multilayer thin film structures and the origin of sensitivity enhancement in surface plasmon resonance sensorsAtef Shalabney,Ibrahim Abdulhalim ∗Department of Electro-Optic Engineering,Ben Gurion University,Beer Sheva 84105,Israela r t i c l e i n f o Article history:Received 6October 2009Received in revised form 8January 2010Accepted 7February 2010Available online 13 February 2010Keywords:Surface plasmon resonance Optical sensors Surface wavesa b s t r a c tThe performance of surface plasmon resonance (SPR)sensors depends on the design parameters.An algorithm for calculating the electromagnetic fields distribution in multilayer structure is developed relying on Abeles matrices method for wave propagation in isotropic stratified media.The correlation between field enhancement and sensitivity enhancement is examined and found to agree with the overlap integral in the analyte region.This correlation was verified in the conventional SPR sensor based on Kretschmann configuration,and in the improved SPR sensor with high refractive index dielectric top layer for several cases,e.g.field enhancement due to resonance,the sensitivity dependence on the wavelength,the influence of prism refractive index on sensitivity,and the effect of the layers materials and thicknesses.© 2010 Elsevier B.V. All rights reserved.1.IntroductionSurface plasmon resonance (SPR)sensors are widely recognized as valuable tools for investigating surface interactions and sensing of gases and biomaterials [1].A surface plasmon wave is a longi-tudinal compressional charge density wave that can propagate at the interface between a metal and a dielectric media under certain conditions.One of the most common techniques for plasmon exci-tation is the Kretschmann configuration shown in Fig.1(a)in which the resonance realized by a dip in the reflectivity versus incidence angle or alternatively in the reflectivity versus wavelength.Since the dip location depends on the substrate (analyte)features,one can measure tiny fluctuations in the refractive index of the analyte by tracking either the shift in the resonance angle or the shift in the resonance wavelength [2–4].Due to the pioneering works of Kretschmann [5]and Otto [6],practical devices were proposed for chemical and biological sensors applications.Several parameters and features define the perfor-mance quality of SPR sensor:(1)reflectance profile shape (dip depth and width),(2)chemical stability of the metal layer,(3)sensor reso-lution,and (4)sensor sensitivity.The sensor’s sensitivity is defined as the ratio between the resonance angle or wavelength shift per analyte refractive index unit.Gent et al.[7]defined the sensitivity∗Corresponding author at:Ben Gurion University,Department of Electro-optic Engineering,P.O.Box 653,84105Beer Sheva,Israel.Tel.:+97286479803.E-mail addresses:shalaban@bgu.ac.il (A.Shalabney),abdulhlm@bgu.ac.il (I.Abdulhalim).as the ratio between the shift and width of the dip.This definition is somehow misleading and questionable because several algorithms can be applied to determine the dip position with high accuracy even with moderate resolution.The later definition was considered by Golosovsky et al.[8]recently.They demonstrated that the sensi-tivity of SPR technique in the infrared range using Fourier transform infrared (FTIR)spectrometry is not lower compared to the sensitiv-ity of the SPR technique in the visible range.In the present work we define the sensitivity as the dip shift per sample refractive index unit and will ignore dip width aspect in the sensitivity definition.In the last two decades few methods were proposed to improve the SPR sensor ing long range SPR (LRSPR)sensor [9,10]where the excitation of two SP waves on the interfaces of the metal layer placed between two dielectric layers increases the propagation distance on the surfaces and correspondingly increases the sensitivity.Although many works reported sensitiv-ity enhancement by measuring phase instead of intensity [11–14],recent study [15]showed that these reports are questionable and the accuracy in phase measurements is limited by the accuracy of intensity measurements.Another method to enhance SPR sensor sensitivity was using periodic metallic structures combined with TIR Kretschmann configuration [16,17].Also the use of bimetallic layers [18–20],and modification of prism refractive index [21,22]were proposed in order to enhance SPR sensor hav et al.presented for the first time the nearly guided wave SPR (NGWSPR)configuration that is similar to the conventional con-figuration with the addition of 10–15nm dielectric layer with a high refractive index between the metal layer and the cover mate-rial (the analyte to be sensed)[23,24]as shown in Fig.1(b).The0924-4247/$–see front matter © 2010 Elsevier B.V. All rights reserved.doi:10.1016/j.sna.2010.02.005A.Shalabney,I.Abdulhalim/Sensors and Actuators A159 (2010) 24–3225Fig.1.(a)Schematic of single layer Kretschmann configuration and(b)schematic of double layer Kretschmann configuration. authors choose silicon(Si)to accomplish the task because Si hashigh real part of refractive hav et al.configuration has twoprominent advantages:first the sensitivity was enhanced by fewtimes compared to conventional SPR sensor,and second the sta-bility of the metal layer was improved because the silicon servedas protection layer for the silver which suffers from a poor chem-ical stability.When the addition of a thin dielectric layer witha high refractive index on top of the metal layer is considered,one should emphasize that the added layer is very thin(typically10nm).One should distinguish this case from the case of coupledplasmon-waveguide resonance(CPWR).CPWR biosensors incorpo-rate a thick waveguide layer(typically500nm)beneath the surfaceof the conventional SPR biosensor[25,26].Unlike the conventionalSPR biosensors,whose reflectivity demonstrates a dip only in theTM mode,the interference in the waveguide layer causes a dip bothin the TE and TM modes in the CPWR device[27].Although CPWRsensors exhibit sharp dips and improve significantly the SNR of themeasurement,their sensitivity is less than that of conventional SPRdevices by an order of magnitude since the biosensing surface islocated at a considerable distance from the SPs which exist on theinterface between the metal and the waveguide layer[28,29].Thecontribution of a thin dielectric top layer to the sensitivity enhance-ment of SPR sensor was reported for thefirst time by Lahav et al.Because the nm-thick layer does not support guided modes,it wascalled nearly guided wave SPR(NGWSPR)sensor.In the present work we investigate the sensitivity enhancementmechanism of SPR sensors by electromagneticfield and energydistribution considerations.Since the SPR is accompanied by anenhanced evanescentfield in the metal–analyte interface region,the sensor sensitivity for a perturbation in the analyte is determinedby thefield distribution in this region.According to Abdulhalim[30],the shift in the wave vector is proportional to the overlap inte-gral which in turn is proportional to the interaction volume V in(seeAppendix A):ık≈k i2V inıε·៝E∗i· E f·drVε·៝E∗i· E i·dr(1)where E i,k i are the electricalfield and its wave vector before the variation in the analyte refractive index took place,while E f is the field after the index perturbation andık is the associated shift in the wave vector due to a change fromεtoε+ıεin the analyte dielectric constant.Sinceık expresses the change in the incidence angle or alternatively the change in the wavelength,thenık/ıεrep-resents the sensitivity of the sensor,which is proportional to the overlap integral in the numerator of Eq.(1)normalized to the total energy.Hence to maximize the sensitivity one needs to maximize this integral which can be accomplished by increasing the inter-action volume,that is the evanescence depth,the SP propagation length along the surface or by increasing thefield intensity in the analyte region.One of our purposes in this paper is to demonstrate that sensitivity enhancement mechanisms are accompanied with field enhancement and correlated with the overlap integral in the analyte region.Full description of the electromagneticfield in multilayer struc-tures within the SPR modes context was done by Davis[31]where the purpose was obtaining the eigenfunctions of the differential equation for the magneticfield distribution and simultaneously the eigenvalues were derived from the dispersion relation.Chin et al.[32]presented calculations for electromagneticfield distribution by matrices approach to extract the reflectivity for the multilayer structure.In both previous works[31,32],they used propagation matrices to calculate the two components of the forward and back-ward propagating magneticfield amplitudes in arbitrary layer from the boundary values of the aforementioned amplitudes.Ohta et al.[33]proposed a method to calculate the forward and backward propagating electricfield without using inverse matrices;however, he described the fullfields by exponential expressions which may be problematic due to some numerical instability.Ekgasit et al.[34] and Hansen[35]used the characteristic matrices approach and the total transmission coefficients for explaining their SPR spec-troscopy experiments;however,they only emphasized absorbance aspects in multilayer system.The uniqueness of our work is in that it involves a detailed simplified algorithm for electromagneticfield distribution and full comprehensive investigation for the evanes-centfield role in sensitivity enhancement which was never done before to the best of our knowledge.2.The algorithm forfield distributionWe consider the general case of plane wave radiation interact-ing with a stack of N−1layers as shown in Fig.2so that we have N interfaces.The layers are homogenous and isotropic with plane boundaries,the optical properties of each layer are characterized completely by two constants which are functions of wavelength, e.g.the dielectric constantεj=n2j where n j the refractive index for layer-j and the magnetic permeability is j.The constantsεj, n j, j are complex in the general case.Under the SPR conditions, the electricfield must have a component which is perpendicular to the surface;hence the incident light should be TM polarized.We take the plane of incidence to be the XZ-plane and the Z-axis is the direction of stratification.For TM wave,H x=H z=0and E y=0.The non-vanishing components of thefield vectors into each layer-j are26 A.Shalabney,I.Abdulhalim /Sensors and Actuators A 159 (2010) 24–32of the form:H yj (x,z,t )=H yj (z )exp i (k 0˛j x −ωt ),E xj (x,z,t )=E xj (z )exp i (k 0˛j x −ωt ),E zj (x,z,t )=E zj (z )exp i (k 0˛j x −ωt ),(2)here H yj (z ),E xj (z ),E zj (z )are the amplitudes of the appropriate fields in layer-j that are in general complex functions of z ;˛j =n j sin Âj when Âj the propagation angle and k 0=2 / 0the wave number in vacuum.According to Snell’s law one can consider the following:˛j =n j sin Âj =const =˛=n 0sin Â0(3)When the general structure shown in Fig.2constitutes an SPR sensor,the substrate and the ambient will represent the ana-lyte and the prism,respectively,to be consistent with Fig.1.As was mentioned in the introduction,methods for calculating field’s distribution in N layered structure were presented by several inves-tigators [33–35],however,as algorithms we found them not clear enough and difficult to use particularly for researchers from the bio-sciences and bio-technology.In the present section we introduce a detailed and simplified algorithm for field distribution calculation.Our description completely detailed to allow convenience numer-ical implementation in three steps.Step 1:Calculating total characteristic matrix,reflection and trans-mission coefficients for the whole structure .The magnetic and electric fields amplitudes in the entrance of each layer are connected to the corresponding amplitudes at a distance z inside the layer by the well known Abeles [36]matrices:H 0yj −E 0xj=M j ·H yj (z )−E xj (z )=⎡⎣cos ˇj −iq j sin ˇj −iq j sin ˇjcos ˇj⎤⎦·H yj (z )−E xj (z )(4)H 0yj ,E 0xjare the amplitudes of H yj ,E xj ,respectively,at the appropriate boundary z =Z j of layer-j ,M j is called the characteristic matrix for the layer-j and it is determined by the optical properties and the layer thickness (d j )where:ˇj =k 0n j d j cos Âj ;q j = ( j /εj )cos Âj.Fig.2.Interaction of plane wave with a multilayer system,the stack composed of(N −1)homogenous and isotropic media with N interfaces that bounded by two semi-infinite homogenous,isotropic,and dielectric media.For a multilayered structure the field amplitudes at the first boundary are connected to those at the last boundary by the total characteristic matrix:H 0y 1−E 0x 1=M TOT ·H 0yN −E 0xN,M TOT =j =N −1j =1M j(5)The complex reflection and transmission coefficients r and t can beexpressed in terms of the elements of the total characteristic matrix of the whole system M ij :r ≡H refyH inc y =(M 11+M 12·q N )·q 0−(M 21+M 22·q N )(M 11+M 12·q N )·q 0+(M 21+M 22·q N )t ≡H 0yN H inc y=2·q 0(M 11+M 12·q N )·q 0+(M 21+M 22·q N )(6)H inc y ,H refy are the incident and reflected magnetic field amplitudes,H 0yN is the transmitted magnetic field amplitude and simultane-ously the amplitude at the last interface (z =Z N ).Step 2:Calculating the field within the 1st layer:H y 1(z )−E x 1(z )=P 1(z )·(1+r )q 0(1−r )H inc y ,Z 1≤z ≤Z 2(7)where P 1(z )is given by:P 1(z )=⎡⎣cos(k 0n 1z cos Â1)iq 1sin(k 0n 1z cos Â1)iq 1sin(k 0n 1z cos Â1)cos(k 0n 1z cos Â1)⎤⎦(8)And the incident magnetic field amplitude maybe taken asunity:H inc y=1for normalized magnetic field,while if one needs to normalize the electric field to the incident electric field ampli-tude,the following substitution H inc y =E inc x/q 0should be used in equations (7)and (9).Step 3:Calculating the field distribution within layer-j ≥2from the following expression:H yj (z )−E xj (z )=P j (z )·⎛⎝1l =j −1P l (z =Z l +d l )⎞⎠·(1+r )q 0(1−r )H inc y ,Z j ≤z ≤Z j +1(9)where P j (z )is the propagation matrix for the layer-j (inverse of thecharacteristic matrix M j (z ))and it is given by:P j (z )=cos(k 0n j (z −Z j )cos Âj )iq jsin(k 0n j (z −Z j )cos Âj )iq j sin(k 0n j (z −Z j )cos Âj )cos(k 0n j (z −Z j )cos Âj )(10)One should be careful when distinguishing between P j (z )and P l (z =Z l +d l )that appear in Eq.(9).While the first is z -dependent as was defined through Eq.(10),the second is the propagation matrix for the layer-l with thickness d l ,which is constant for layer-l .Normalizing to the incidence magnetic field amplitude could beachieved by setting H inc yto unity.Since in many cases the SPR sensor structure is composed of a single metal layer which is embedded between two semi-infinite dielectric media,namely,the prism and the analyte,the distribution expression in this case was separated and represented by step 2.For TE polarization all the expressions for the characteristic and propagation matrices and reflection or transmission coefficients are valid simply by replacing the expression for q j = ( j /εj )cos Âjwith p j =(εj / j )cos Âj and the field column for TE isE yjH xj.A.Shalabney,I.Abdulhalim /Sensors and Actuators A 159 (2010) 24–32273.ApplicationsOur 2nd purpose in this work is to investigate the origin of sensitivity enhancement in SPR sensors based on Kretschmann con-figuration.From Eq.(1)the sensitivity proportional to the ratio between the energy flow in the analyte region and the total energy.In this sense we intend to examine the correlation between sensi-tivity enhancement and the field’s distribution for several cases.In part of the cases,we deal with the conventional configuration as shown in Fig.1(a),and afterwards with the improved configuration as shown in Fig.1(b).In the simulations the resonance angle was calculated with accuracy of Â=0.001◦and the variation in the analyte refractive index was n a =0.01RIU.Hence the sensitivity accuracy obtained to be S =0.01◦/RIU.For field calculations,the amplitudes were computed in steps of z =0.5nm.3.1.Evanescent field treatment for the standard Kretschmann configurationFirst we consider the basic configuration shown in Fig.1(a).The surface plasmon wave at the metal/dielectric interface is excited if the wave vector in X -direction of the incident wave matches that of the surface plasmon.When the surface plasmon is excited,a sub-stantial decrease in reflectance is observed at the resonance angle,Âr as shown in Fig.3(a).The resonance angle,Âr depends on theanalyte refractive index,n a =√εa so that a change in the refrac-tive index causes an appropriate shift in the resonance angle,see Fig.3(b).The ratio between the angle shift and the refractive index change is defined as the angular sensitivity S Â(S Â=d Âr /dn a ).At the resonance,the reflectivity R reaches its minimum value,the intensity of the electromagnetic field reaches its maximum at the surface,see Fig.4.Near the resonance angle,an extremely strong evanescent field at the metal/dielectric interface is gener-ated by the surface plasmon wave.The unique characteristic of generating evanescent filed,where the field amplitude is greatest at the interface and exponentially decaying as a function of dis-tance from the metal/dielectric interface,makes the SPR signal very sensitive to changes at the vicinity of the metal surface.The X -component of the electric field is continuous;however,the Z -component is discontinuous.Due to the small dielectric con-stant of the analyte (εa )compared to that of the metal (|εm |),|E z |has a strong enhancement at the metal/analyte interface.As shown in Fig.4(a)and (b)the enhancement of the electric field is largest at the resonance compared to the cases near the resonance,where the resonance angle in this case is Âres =54.619◦.Since there is a need sometimes of detecting at various wave-lengths,sensitivity versus wavelength investigation is essential.As shown in Fig.5(a),the sensitivity of the configuration describedin Fig.1(a)decreases when the wavelength increases.This result was presented by Homola [37]for the Kretschmann configuration without physical interpretation.Now in terms of the evanescent field,we can attribute the high sensitivity for lower wavelength to the larger interaction of the electric field in the analyte region.Although for larger wavelengths there is a larger penetration depth into the analyte region,the amplitude becomes smaller,which indi-cates that sensitivity is not governed only by the penetration depth,see Fig.5(b),rather by both the interaction region and the energy distribution as expressed by the overlap integral of Eq.(1).The sensitivity versus wavelength was calculated after per-forming optimization of the metal layer thickness.The silver layer thickness was chosen such that the resonance condition is preserved for each examined wavelength and the reflectivity at the dip is less than 0.01.As seen from the caption of Fig.5,the silver thickness is decreasing when increasing wavelength.The losses in the metal increases with wavelength,and in order to overcome these losses and preserve resonance condition,the thickness should be reduced.In this case the sensitivity is approx-imately constant for large wavelengths,because in the IR range the sensitivity is mainly governed by the difference between the prism refractive index and the analyte refractive index S Â=(dÂ/dn a )−→ →∞(1/ n 2p −n 2a )(rad/RIU).3.2.Evanescent field treatment for SPR sensor with nm-thick topdielectric layerAs pointed our before,the addition of a thin dielectric layer with a high real part of the refractive index causes substantial enhancement in the sensor sensitivity.Silicon was chosen for its high real part of the refractive index because the sensitivity enhancement was found [23,24]to increase with the real part of the dielectric constant of the top layer.As an optimization process for the silicon layer thickness,d Si =10.5nm is the opti-mum thickness for maximum sensitivity as shown in Fig.6(a).The evanescent field distribution demonstrates that for this silicon layer thickness,maximum enhancement ratio for the amplitude E x is obtained.The addition of the silicon layer increases the sensitiv-ity by threefold,e.g.67.5◦/RIU sensitivity without the silicon layer,compared with 200◦/RIU sensitivity with d Si =10.5nm silicon top layer.The optimum silicon thickness of d Si =10.5nm was chosen for 43nm silver layer thickness because it gave the highest sensitiv-ity enhancement.As done before with the standard Kretschmann configuration,one can perform an optimization for the improved sensor with the silicon dielectric layer.The sensitivity dependence on the wavelength has been examined for the improved sensor,and the same proportion was obtained.As shown in Fig.7(a)and (b),a similar behavior for the sensitivity versus wavelength isobtained,Fig.3.(a)Reflectivity as a function of incidence angle for the configuration shown in Fig.1(a).(b)Reflectivity versus analyte refractive index for the configuration shown in Fig.1(b). =633nm,d m =43nm,n p =1.732,Âr =54.61◦,n a =1.325(blue curve),n a =1.335(red curve),silicon refractive index at 633nm is n s =3.8354+0.0245i and the silver refractive index is n s =0.1325+4.0203i .(For interpretation of the references to color in this figure legend,the reader is referred to the web version of the article.)28 A.Shalabney,I.Abdulhalim /Sensors and Actuators A159 (2010) 24–32Fig.4.(a)The density distribution of the X -component of the electric field.(b)The density distribution of the Z -component of the electric field through the layers at different incidence angles at and near the resonance.Both graphs correspond to the following parameters: =633nm,d m =43nm,n a =1.33,n p =1.732,Âr =54.61◦.Fig.5.(a)Sensitivity versus wavelength corresponding to the configuration in Fig.1(a).(b)The density distribution of the electric field X -component along the metal and the analyte regions for different wavelengths at the resonance.d m =43nm,n a =1.33,n p =1.732.The thicknesses for obtaining resonance in each wavelength are:47,43.25,and 29.75nm for 632,850,and 1550nm,respectively.except that in the case with the silicon layer the sensitivity starts from a higher value compared to the case without the silicon layer.The origin of the sensitivity enhancement with decreasing the operation wavelength is basically related to the metal behavior.Both the real part and the imaginary part of the metal refractive index are responsible for the transparency features of the metal layer and they become larger when increasing the wavelength.Increasing the real part makes the metal more reflective from the initial prism/metal interface,whereas increasing the imagi-nary part makes it more absorptive and as a result of the double effect the evanescent field experiences more attenuation when it propagates through the whole system.When the refractive index of the metal changes from 0.12+3.75i at 600nm wavelength up to 0.53+10.43i at 1500nm,the amplitude of the fields at the analyteinterface are significantly attenuated and consequently the sensi-tivity of the structure becomes moderate.Although the penetration depth was found to be 94and 697nm at 600and 1500nm wave-lengths,respectively,in the conventional Kretschmann SPR sensor configuration,the sensitivity is still larger for smaller wavelength.The values were calculated from the following expression of the penetration depth:ıd =4εa +εmr −ε2a(13)Here ıd is the penetration depth inside the analyte layer, ,εa ,εmrare the wavelength,analyte dielectric constant,and the metal real part dielectric constant,respectively.This last fact indicatesthatFig.6.(a)Sensitivity versus silicon layer thickness corresponding to the configuration in Fig.1(b).(b)Density distribution of the electric field X -component for different Si layer thicknesses.A.Shalabney,I.Abdulhalim/Sensors and Actuators A159 (2010) 24–3229Fig.7.(a)Sensitivity versus wavelength in the Kretschmann configuration with the silicon layer shown in Fig.1(b).(b)The density distribution of the electricfield X-component through the layers at different resonance wavelengths.The silicon refractive indices are:3.64+0.0085i;3.59+0.0057i;3.4+0.0002i;3.22+0.002i at740,780, 1000and1600nm,respectively,and(n a=1.33,n p=1.732).The silver and silicon thicknesses in this case were chosen under the resonance condition R min<0.01for each wavelength.Table1shows the corresponding thicknesses and sensitivities for(b).Table1Silver and silicon layers thicknesses that were chosen for each wavelength that appear in Fig.7(b)in order to preserve R min<0.01as a condition for resonance.The sensitivity and the E x intensity at the analyte interface were calculated for each wavelength.(nm)wavelength d m(nm)metal thickness d s(nm)silicon thickness Sensitivity(◦/RIU)Field intensity at the analyte interface74045.51720410378044.5202101411000402510536160029548225sensitivity as it was defined in the present study is not governed only by the penetration depth.Absorption considerations will be further discussed in Eqs.(14)and(15)in Section3.3.Sensitivity versus wavelength with the silicon top layer was calculated when the structure was optimized to fulfill the resonance condition at each wavelength.Table1shows the combinations(d m,d s)of the silver and the silicon thicknesses that maintain the structure at resonance,with reflectivity dip level of R min<0.01.3.3.Sensitivity enhancement due to prism refractive indexmodificationThe prism refractive index has an important role in the sensi-tivity determination of the SPR sensor based on TIR configuration. Two works[21,22]were recently published on this issue,and both showed that the sensitivity increases with decreasing the prism refractive index.Thefirst work was on the sensitivity enhancement with the angular interrogation mode[21],in which the authors demonstrated the prism influence without giving any physical explanation for the phenomenon.In the second work which was done by Yulk et al.[22],the spectral interrogation mode was con-sidered and the sensitivity enhancement was attributed to the large penetration depth obtained in the case of small prism refractive index.Under the last hypothesis,the sensitivity for a large wave-length should be larger than the sensitivity for smaller wavelength for all the cases,while we showed the opposite in the former discussion when we examined the wavelength influence on the sensitivity.Hence the explanation that was given by Yulk et al.is inadequate in our opinion.Our approach is based on the correla-tion between sensitivity and overlap integral in the analyte region. Larger enhancement for the electricfield at the metal/dielectric interface for smaller prism refractive index was observed as shown in Fig.8.Fig.8(a)and(b)clearly demonstrates the correlation between the sensitivity enhancement and thefield enhancement.The case demonstrated by Fig.8(a)and(b)relates tofixed opera-tion wavelength( =632nm)while the variation is in the prism refractive index which in turn varies the incidence angle.The sensitivity versus prism refractive index was calculated when the metal layer thickness is optimized under the resonance con-dition(R min<0.01),where R min is the dip level at resonance. Considering the Kretschmann configuration,the resonance condi-tion obtained by equating the SP wave vector and the emerging light wave vector which can explicitly be written as k0n p sinÂ= k0(εmr n2a/(εmr+n2a))where n a and n p are the analyte and the prism refractive indices,respectively,andεmr is the real part of the metal refractive index.By analyzing the last condition one can con-clude that the coupling condition is fulfilled if|εmr|is higher than the quantityÁ=(n2a·n2p/(n2p−n2a))which corresponds to having the resonance anglesÂ≤90◦.For afixed wavelength,the last con-dition creates a singularity in the sensitivity for a prism refractive index which allows the quantityÁto approach|εmr|at the given wavelength.The last interpretation properly explains the dramatic increase in the sensitivity for n p≈1.41in Fig.8(a)for a wavelength of633nm.In a similar manner the influence of the prism refrac-tive index was investigated for the NGWSPR,and similar behavior of the sensitivity versus prism refractive index was found as the conventional SPR sensor.Results are shown in Fig.9(a)and(b).In the case of NGWSPR,there is a shift in the cut-off wavelength which can be clearly seen in the resonance angle positive shift due to the addition of the silicon layer.Basically,the incident light feels a higher refractive index beyond the metal layer and therefore,an appropriate modification in the dispersion relation of the SP.Since the wavelength is keptfixed,the resonance condition is satisfied at larger angle.The sensitivity in the NGWSPR case was calculated when the silver–silicon structure is optimized to maintain the res-onance condition(R min<0.01);the values correspond to thefield intensity distribution in Fig.9(b)are given in Table2.Enhancing thefields at the metal interface increases the absorp-tion as well as increasing the sensitivity of the structure.For conventional SPR sensor without dielectric layer,the only absorb-ing medium in the system is the metalfilm.When adding the silicon layer,the absorption will be both in the metal and the silicon layer. The absorption in the whole system can be expressed by the fol-。
人脸识别技术观点英语作文Here is an English essay on the topic of facial recognition technology, with the content exceeding 1000 words as requested. The essay is written without a title and without any additional punctuation marks in the main body.Facial recognition technology has become increasingly prevalent in our modern society with its wide-ranging applications across various sectors. This advanced biometric identification system utilizes algorithms to map an individual's facial features and compare them against a database of stored facial profiles. While the technology offers numerous benefits in terms of security enhancement and convenience, it also raises significant ethical and privacy concerns that warrant careful consideration.One of the primary advantages of facial recognition technology is its ability to enhance security measures. In the realm of law enforcement, this technology can aid in the identification and apprehension of criminals, potentially leading to improved public safety. By cross-referencing facial data with criminal databases, authorities can quickly and accurately pinpoint suspects, streamlining investigative processes. Additionally, this technology can beimplemented in secure access control systems, ensuring that only authorized individuals can gain entry to sensitive areas or facilities, thereby reducing the risk of unauthorized access.Furthermore, facial recognition technology has proven invaluable in various commercial and social applications. Retailers can leverage this technology to personalize customer experiences, offering targeted advertisements and recommendations based on individual preferences. In the financial sector, banks can utilize facial recognition for secure authentication, reducing the reliance on traditional password-based systems and enhancing the overall security of transactions. Similarly, social media platforms can employ facial recognition to facilitate features such as automatic tagging and photo organization, improving user experience and engagement.However, the widespread adoption of facial recognition technology also raises significant ethical and privacy concerns. One of the primary issues is the potential for infringement on individual privacy. The collection and storage of biometric data, such as facial profiles, without explicit consent or adequate safeguards, can be perceived as a violation of an individual's right to privacy. This concern is amplified by the possibility of unauthorized access or misuse of this sensitive information, which could lead to identity theft, stalking, or other malicious activities.Moreover, the accuracy and reliability of facial recognition systems have come under scrutiny. Studies have shown that these systems can exhibit biases, often performing less accurately for individuals from certain demographic groups, such as women and people of color. This bias can lead to disproportionate targeting or false identifications, which can have severe consequences, particularly in law enforcement contexts. The potential for such errors to perpetuate societal inequalities and undermine the principles of fairness and justice is a significant concern.Another pressing issue is the lack of comprehensive regulatory frameworks governing the use of facial recognition technology. In many countries, the legal landscape surrounding the collection, storage, and application of biometric data remains unclear, leaving individuals vulnerable to potential abuses. Without clear guidelines and oversight, there is a risk of unchecked surveillance, profiling, and discrimination, which can erode the fundamental civil liberties and democratic principles that societies strive to uphold.Furthermore, the potential for the misuse of facial recognition technology by authoritarian regimes or malicious actors is a grave concern. Unchecked access to this technology could enable the monitoring and suppression of dissent, the targeting of minority groups, and the erosion of freedom of expression and association. The dystopian scenarios envisioned in works of science fiction havethe potential to become a reality if appropriate safeguards and ethical considerations are not prioritized.In response to these concerns, there have been growing calls for greater regulation and oversight of facial recognition technology. Policymakers and civil society organizations have advocated for the implementation of robust privacy laws, data protection frameworks, and algorithmic accountability measures. These efforts aim to ensure that the development and deployment of facial recognition systems are aligned with fundamental human rights and that individuals are granted the necessary protections against potential abuses.Additionally, there have been calls for increased transparency and public discourse around the use of facial recognition technology. Engaging with diverse stakeholders, including privacy advocates, technology experts, and affected communities, can help shape policies that strike a balance between the benefits of the technology and the preservation of individual rights and civil liberties.It is also crucial that the developers and deployers of facial recognition systems prioritize the principles of fairness, non-discrimination, and ethical design. This includes addressing the issue of algorithmic bias, improving the accuracy and reliability of the technology, and implementing rigorous testing and auditing procedures to identify and mitigate potential harms.In conclusion, the rapid advancement of facial recognition technology presents both opportunities and challenges. While the technology offers valuable applications in enhancing security and enabling convenient commercial and social experiences, the ethical and privacy concerns it raises cannot be overlooked. Striking the right balance between the benefits of the technology and the protection of individual rights will require a multifaceted approach involving robust regulation, transparent governance, and a commitment to ethical and responsible development. As we navigate the evolving landscape of facial recognition technology, it is crucial that we prioritize the preservation of fundamental human rights and the well-being of individuals and society as a whole.。
人的感知的塑造的作文英文回答:Perception is a fascinating aspect of human psychology. It plays a crucial role in shaping our understanding of the world around us. Our perception is influenced by various factors, including our senses, experiences, and cultural background.Our senses, such as sight, hearing, taste, smell, and touch, provide us with the initial input that our brain processes to form a perception. For example, when we see a red apple, our eyes detect the wavelengths of light reflected by the apple, and our brain interprets it as the color red. However, it is important to note that our senses are not always accurate. Optical illusions, for instance, demonstrate how our perception can be easily manipulated.Experiences also play a significant role in shaping our perception. Our past experiences create a framework throughwhich we interpret new information. For example, if someone had a negative experience with dogs in the past, they may perceive all dogs as dangerous or threatening. On the other hand, someone who has had positive experiences with dogs may perceive them as friendly and loyal companions.Cultural background also influences our perception. Different cultures have different beliefs, values, and norms, which can affect how individuals perceive certain situations or behaviors. For instance, in some cultures, direct eye contact is seen as a sign of respect, while in others it may be perceived as confrontational. These cultural differences can lead to misunderstandings and misinterpretations.In addition to these factors, our perception is also influenced by cognitive biases. These biases are mental shortcuts that our brain takes to process information quickly. However, they can sometimes lead to errors in judgment. For example, confirmation bias is the tendency to search for or interpret information in a way that confirms our preexisting beliefs. This can lead to a distortedperception of reality.Overall, our perception is a complex interplay of our senses, experiences, cultural background, and cognitive biases. It is a subjective and individualized process that shapes how we understand and interpret the world. It is important to be aware of these factors and to approach situations with an open mind, considering different perspectives and challenging our own biases.中文回答:感知是人类心理学中一个迷人的方面。
关于面孔知觉的英文文献Face Perception: A Cognitive and Computational Perspective.Introduction.Face perception is a fundamental aspect of human cognition, enabling us to recognize and interact with others, navigate social environments, and express emotions. It is a complex process that involves multiple cognitive and computational mechanisms, including visual processing, attention, memory, and decision-making.Visual Processing.The visual system plays a crucial role in face perception. When we encounter a face, the eyes first scan the central features, such as the eyes, nose, and mouth. This information is then rapidly processed by the visual cortex, which extracts key features and attributes, such asfacial shape, size, and texture.Attention.Attention is essential for focusing on specific aspects of a face, such as the eyes or mouth, which conveyimportant social and emotional information. Attention canbe influenced by a range of factors, including task demands, social cues, and personal preferences.Memory.Memory plays a vital role in face recognition. We store representations of familiar faces in our memory, which allows us to recognize them even after long periods of time. These representations include both structural information about facial features and associated semantic information, such as names and relationships.Decision-Making.Face perception ultimately involves making decisionsabout identity, emotion, and social intentions. These decisions are based on the integration of visual, attentional, and memory processes. For example, when we see a friend's face, we may recognize them based on their unique facial features and associate them with a name and personality.Computational Models.Computational models have been developed to simulate the processes involved in face perception. These models aim to explain how different cognitive and computational mechanisms interact to produce accurate and meaningful representations of faces. Some of the most widely used models include:Face Recognition Networks: These models use machine learning algorithms to learn the features that distinguish different faces. They can be trained on large datasets of images and achieve impressive performance on face recognition tasks.Geometric Face Models: These models represent faces as 3D objects, allowing for the extraction of detailed structural information. They can be used for facial animation, facial reconstruction, and other applications.Statistical Face Models: These models capture the statistical regularities of faces, such as the typical shape and distribution of facial features. They can be used to generate realistic face images and for facial recognition tasks.Applications.Face perception has numerous applications in various fields, including:Security and Surveillance: Face recognition systems can be used for access control, surveillance, and criminal identification.Medical Diagnosis: Facial features can provide clues about certain medical conditions, such as genetic disordersand neurological damage.Human-Computer Interaction: Face-tracking technology allows computers to interact with users in a more natural and intuitive way.Social Psychology: Face perception plays a critical role in social interactions, such as forming impressions, understanding emotions, and establishing relationships.Conclusion.Face perception is a complex and multifaceted cognitive process that involves visual processing, attention, memory, and decision-making. Computational models have provided valuable insights into the mechanisms underlying face perception, leading to numerous applications in various fields. As research continues, our understanding of this fundamental aspect of human cognition will continue to deepen.。
光照灵敏度英文English:Photoperiod sensitivity, also known as photoperiodism, refers to the way in which an organism responds to changes in day length. Different species exhibit varying levels of sensitivity to changes in day length, with some being classified as long-day plants, short-day plants, or day-neutral plants. Long-day plants require longer periods of daylight to initiate flowering, while short-day plants require shorter periods of daylight. Day-neutral plants, on the other hand, are not affected by changes in day length. The sensitivity to changes in day length is controlled by specific genes that regulate the production of flowering hormones. In addition to plants, many animals also exhibit photoperiod sensitivity, which can influence their behavior and physiological processes. Overall, photoperiod sensitivity plays a crucial role in determining the life cycle and reproductive success of various organisms in different environments.Translated content:光期敏感性,也被称为光期效应,指的是生物对白昼长度变化作出反应的方式。
鲨鱼表皮的仿生原理对人类的作用英语作文The biomimetic principle of shark skin and its impact on humanityIntroduction:Over the years, scientists have studied and imitated nature to solve various engineering challenges. One of the most fascinating examples of biomimicry is shark skin, which has inspired numerous innovations that have benefited humanity in various ways. In this essay, we will explore the biomimetic principle of shark skin and its impact on human society.Shark skin:Shark skin is covered with tiny V-shaped scales called dermal denticles. These scales reduce drag and turbulence in the water, allowing sharks to swim faster and more efficiently. The rough texture of shark skin also prevents the growth of algae and other organisms, reducing the need for cleaning and maintenance.Biomimetic applications:Inspired by the efficiency of shark skin, scientists and engineers have developed various technologies that mimic its structure and function. One such innovation is the Sharklet™technology, which uses a textured surface to inhibit the growth of bacteria and other harmful microorganisms. This technology has been applied to medical devices, hospital surfaces, and even clothing to reduce the spread of infections and improve hygiene.Another example of biomimicry inspired by shark skin is the development of drag-reducing coatings for ships and airplanes. By imitating the rough texture of shark skin, these coatings reduce friction and increase fuel efficiency, leading to significant cost savings and environmental benefits.Impact on humanity:The biomimetic principle of shark skin has had a profound impact on human society in various ways. It has improved hygiene and reduced the spread of infections in healthcare settings, ultimately saving lives and improving patient outcomes. It has also helped to increase the efficiency and sustainability of transportation systems, leading to economic benefits and environmental protection.Furthermore, the study of shark skin has inspired new research in biomimetics, leading to innovations in a wide range of fields, from materials science to robotics. By looking to nature for inspiration, scientists and engineers are constantly findingnew solutions to complex problems and pushing the boundaries of human knowledge and innovation.Conclusion:In conclusion, the biomimetic principle of shark skin has had a transformative impact on humanity, leading to technological advancements that improve efficiency, sustainability, and health. By studying and imitating the incredible design of nature, we can continue to unlock new possibilities and create a better future for ourselves and our planet.。
2023外研版-高二上册-成都第七中学-(第二次月考模拟试卷)(本试卷不含听力)题号A卷B卷总分得分注意事项:1.答卷前,考生务必将自己的姓名、准考证号填写在答题卡上。
2.回答选择题时,选出每小题答案后,用铅笔把答题卡上对应题目的答案标号涂黑。
如需改动,用橡皮擦干净后,再选涂其他答案标号。
回答非选择题时,将答案写在答题卡上,写在本试卷上无效。
3.考试结束后,将本试卷和答题卡一并交回。
第一部分阅读[共两节,满分50分]第一节(共15小题;每小题2.5分,满分37.5分)阅读下列短文,从每题所给的A、B、C、D四个选项中选出最佳选项。
A篇This document sets out the display standards for Glasgow Museums. This guide will help exhibition planners provide access to exhibitions in our museums. Glasgow Museums’ aim is to improve access to collections by having as many items as possible on display and without physicalbarriers. We also try our best to protect these objects without limiting access to them.Object Placement*Don’t place objects in such a way that they could present a danger to visitors.*All object displays, cased or otherwise, must be viewable by all, including people who are small in figure or in wheelchairs.Open Display*All objects on open display must be Secure from theft and damage.*All objects identified for potential open display must be viewed and agreed on an object-to-object basis byRecommendations DistanceRecommended distance to place objects out of “casual arm's 700mm length”700mm(taken from the edge of the object to the edge of any proposed form ofbarrier)Cased Objects*All cased displays should fall within the general optimum(最优的)viewing band of 750-2,000mm. Ensure everything is visually accessible from a wheelchair.*Position small objects or those with fine detail in the front part of a case, with larger items behind.*Position small items or those with fine detail no higher than 1,015mm from floor level. Objects placed abovethis height are only seen from below by people in wheelchairs or people who are small in figure.( )1. From this text, we can learn that Glasgow Museums______.A. limit access to exhibitions on a daily basisB. are most well-known for its large collectionC. make generous donations to the disabledD. give weight to the experiences of visitors( )2. According to the guide, objects to be placed on open display must______.A. be equipped with anti-theft systemB. be viewed from a distance of 700mmC. receive approval from the museum firstD. fall within arms' reach of a standing man( )3. A mother and her 10-year-old son are likely to both feel comfortable in front of a diamond placed in a glass case at the height of______.A.1,250mmB.950mmC.650mmD.450mmB篇In mammals, loud calls usually serve as alarm signals that warn fellow species of an enemy or other danger. On the other hand, screams from humans can have very different meanings, as Swiss researchers now report in the journal“PLoS Biology”. Human’s screams are not always only associated with negative emotions such as fear, pain, anger and grief, but also positive emotions such as joy and pleasure.Sascha Frihholz and his colleagues from the University of Zurichstudied how many types of human screams there are, how accurately test subjects can distinguish them, and which brain regions are involved(参与)in processing such sound signals. To do this, they first made sound recordings of different screams. Twelve men and women were to put themselves in situations where they reacted with a brief scream. The predetermined situations included, for example: an attack by an armed stranger, celebration about a sporting victory of their favorite team, the threat of an opponent.From the recordings, the researchers finally selected 420 screams. From this, they identified six distinct categories of screams, including three of alarming characters (cries of pain, anger, and fear) and three non-alarming screams ( as expressions of great pleasure, extreme joy, and desperate sadness). Contrary to all expectations, the subjects reacted to non-alarming screams more quickly and recognized the emotion expressed with it more reliably than with alarming screams. This was also confirmed by images of the brain using functional magnetic resonance imaging(FMR).“Until now, researchers assumed that humans also detect and process perceived alarm signals particularly quickly in the form of screams, as this is an important survival mechanism(机制),”says Frihholz. But unlike monkeys and other mammals, non-alarming screams would have become more important for communication.“This changed priority(优先)is probably due to needs that have evolved in the evolution of complex human social relationships."( )4. What does the text mainly talk about?A. Human’s screams expressions.B. An experiment launched in screams.C. Loud calls as a survival mechanism.D. Mammals' loud calls served as alarm signals.( )5. Which expression can be reacted to more quickly?A. Cries of pain.B. Anger.C. Fear.D. Desperate sadness( )6. What does the last paragraph convey?A. Monkeys are not sensitive to non-alarming screams.B. Humans survive because of quick reaction to screams.C. Non-alarming screams gain priority because of human’s revolution.D. Non-alarming screams make human social relationships more complex.( )7. What is the purpose of the text?A. To spread and advocate.B. To argue and discuss.C. To compare and inform.D. To recommend and introduce.C篇Good manners are always good manners. That's what I thought until I married Alexander, who is Russian.When I first met Alexander and he said to me in Russian, “Nalei mnye chai-pour me some tea.” I got angry and answered, “Pour it yourself." Translated into English, without a“please”, it sounded really rude to me. But in Russian it was fine-you don't have to add any polite words.However, when I took Alexander home to meet my parents in the UK, I had to give him a good lesson about pleases and thank-yous, and to teach him to smile, smile, smile.Another thing that Alexander just couldn't understand was why people say things like “Wouldyou mind passing me the salt, please?" He said, “It's only the salt, for God’s sake! What do you say in English if you want a real favour?”He also watched in amazement when, at a dinner party in England, we swallowed some really disgusting food and I said, “Mmmm... delicious." In Russia, people are much more direct. The first time Alexander's mother came to our house for dinner, she told me that my soup needed more flavouring. Afterwards, when we argued about it, my husband said, “Do you prefer your dinner guests to lie?"Alexander complained that in England he felt like an idiot because in Russia if you smile all the time people think you are mad. In fact, this is exactly what my husband's friends thought of me the first time I went to Russia because I smiled at everyone.At home we now have an agreement. If we're speaking Russian, he can say “Pour me some tea”. But when we're speaking English, he has to add a “please”, a “thank you”, and a smile.( )8.What can we know from what Alexander said?A. He didn't think politeness was necessary.B. He didn't like the writer's politeness.C. He wasn't used to the English politeness.D. He wasn’t willing to have good manners.( )9. What did Alexander's friends think of the author when they first met her?A.She was noble.B. She was strange.C. She was lovely.D. She was impolite.( )10. What can we learn from their agreement?A. They respect each other.B. They change a lot for each other.C. They learn from each other.D. They fail to fit in with each other.( )ll. What topic is the text mainly about?A. Good manners.B. Human relations.C. Culture shock.D. Mixed marriages.D篇What fisherman Moul Thun from a remote island in the Mekong River, in northern Cambodia, didn't know was that stingray(黄貂鱼) he hooked would eventually be named the largest recorded freshwater fish. For Zeb Hogan, who’s been documenting large freshwater fishes for almost two decades, the discovery of the stingray, which was released alive back into the river, filled him with hope. "It proves these underwater big fish, which are in critical danger, still exist," says Hogan.Hogan's pursuit for big fish, called the Megafishes Project and supported by National Geographic Society, began in 2005 when fishermen in northern Thailand pulled a 646 pound catfish out of the Mekong River. The species is known that it was the largest, that is, the heaviest-ever caught in the area.Arriving at the island, the team found Thun's fish, a female that appeared to be in good health. It was more than 13 feet from nose to tail. The researchers were shocked to see her weight at 661 pounds. She set a new world record. The original aim of the Megafishes Project was to find, study, and protect the world's largest freshwater fishes. The project focused on species that could grow to at least the size of a human and that lived only in freshwater.Hogan initially drew up a list of roughly 30 species to focus on.The challenge, as Hogan soon learned, was that many of these fish are hard to find. They live in remote, inaccessible places, and often in deep waters. Early on in the search, there were relatively few scientists studying them.What was clear was that the river giants were shrinking in number, threatened by a host of factors including overfishing, water pollution, and the presence of dams, which block migrating fish from completing their life cycles. As Hogan's work progressed, its focus increasingly turned to conservation. “It was never about just finding the biggest fish,” Hogan says, “but looking for ways to protect these extraordinary animals that, in some cases, have been on Earth for hundreds of millions of years but are now drifting out of entities."( )12. Why was Zeb Hogan full of hope?A. Freshwater fishes weren’t in danger.B. Some large fishes didn’t die out.C. The stingray was put into the river.D. Many large fishes existed in rivers.( )13. What can we know about the 646-pound catfish?A. It was also caught by Moul Thun.B. It's the largest recorded freshwater fish.C. It was injured very seriously.D. It was caught in the Mekong River.( )14. Which of the following is the original goal of the Megafishes Project?A. Finding about 30 species of big fishes.B. Studying fishes smaller than humans.C. Protecting big freshwater fishes.D. Setting new record in finding fishes.( )15. What does the underlined part “drifting out of entities" in the last paragraph mean?A. Dying outB. Getting illC. Being protected.D. Living well.第二节(共5小题;每小题2.5分,满分12.5分)阅读下面短文,从短文后的选项中选出可以填入空白处的最佳选项。
The Wonders of the Human Skills The wonders of human skills are truly remarkable and have been a source of fascination for centuries. From the intricate movements of a skilled dancer to the precise strokes of a talented artist, human skills encompass a wide range of abilities that never cease to amaze. In this essay, we will explore the diverse realms of human skills, from the physical to the cognitive, and delve into the significance and impact of these abilities on individuals and society as a whole. One of the most captivating aspects of human skills is the sheer diversity and depth of talent that exists within the human population. Each person possesses a unique set of skills and abilities, honed through years of practice, dedication, and passion. Whether it's the dexterity of a master pianist, the agility of a professional athlete, or the creativity of a visionary architect, human skills encompass a vast spectrum of aptitudes that enrich the world in countless ways. Moreover, human skills are not limited to the physical realm but also extend to the cognitive and emotional domains. The ability to think critically, solve complex problems, and express empathy are all integral components of human skills that contribute to the advancement of society. The capacity for innovation, creativity, and emotional intelligence sets humans apart and enables them to tackle challenges, cultivate meaningful relationships, and make significant contributions to the world. Furthermore, the development and mastery of human skills often require a considerable amount of time, effort, and perseverance. Whether it's the countless hours spent in the studio perfecting a dance routine, the rigorous training sessions of an aspiring athlete, or the continuous learning and adaptation of a skilled professional, the cultivation of human skills demands unwavering commitment and resilience. This dedication not only hones the specific skill set but also fosters discipline, determination, and a strong work ethic that permeates all aspects of life. In addition, the impact of human skills extendsfar beyond the individual level and plays a pivotal role in shaping the fabric of society. The collective culmination of human skills fuels progress, innovation, and cultural enrichment, driving advancements in technology, science, art, and numerous other fields. Moreover, the sharing of skills and knowledge within communities and across generations fosters a sense of unity, cooperation, andmutual support, creating a vibrant tapestry of human achievement and interconnectedness. Furthermore, the celebration and recognition of human skills serve to inspire and uplift individuals, instilling a sense of pride, purpose, and belonging. Whether it's the accolades bestowed upon a talented performer, the admiration directed towards a skilled artisan, or the respect accorded to a knowledgeable mentor, the acknowledgment of human skills fosters a culture of appreciation and encouragement. This validation not only validates the efforts and dedication of individuals but also encourages others to pursue their passions and develop their own unique skills. In conclusion, the wonders of human skills encompass a rich tapestry of abilities that span the physical, cognitive, and emotional realms. The diversity, dedication, and impact of human skills underscore the profound significance of these abilities in enriching the lives of individuals and shaping the collective landscape of society. As we continue to marvel at the remarkable feats of human skill and endeavor, let us cherish, cultivate, and celebrate these extraordinary gifts that define and unite us as a species.。
应用SPR技术揭密HIV前言自1990年推出了第一台商业化的SPR生物传感器-Biacore,Biacore对HIV的研究做出了很大的贡献。
值此在我们又面临新的病源——SARS病毒的威胁之际,我们特地翻译了这篇《应用SPR技术揭密HIV》[1]的综述,望能给各位专家,老师和同道提供有益的启示。
近年来,表面等离子共振(SPR)技术被广泛地应用在与HIV相关的各种功能的研究中,加深了人们对HIV的认识。
无论是蛋白质,核算,脂类,碳水化合物,还是小分子化合物,SPR生物传感器都能实时地分析其相互作用。
而且不需要对分析物进行任何标记。
因此,这项技术被用来分析病毒生命周期每个阶段的各种生物分子间的相互作用,从病毒侵染,复制,出芽到成熟每个阶段的与疫苗开发和抑制药物研究相关的基础研究。
SPR生物传感器提供一个独特和细致的视角来深入了解HIV。
病毒生命周期的每个过程都由分子间的相互作用来控制,也是研究HIV的核心问题。
因此大量的科研工作在SPR生物传感器上完成也就不足为奇了。
如Fig.1a所示,将参与结合的一种伙伴分子例如抗体固定在传感片表面,另一种伙伴分子(在这个例子中为抗原)流过传感片表面。
它们结合形成复合物以及复合物的解离都会改变传感片表面的折光率,SPR能连续地监测这种折光率的变化。
SPR生物传感器对分子质量的检测灵敏度特别高,因此不必对待测样品进行荧光或同位素标记就能识别蛋白质的多个结合位点,还能定量的测定生物分子反应的动力学数据,亲和力和热力学常数。
与其他检测结合反应的技术不同,如酶联免疫反应(ELISA)或基于小珠的技术要求反映物与产物分开,且反应平衡受到洗脱的影响;SPR生物传感器能在存在游离反应物的情况下检测反应复合物的产量,实现了对亲和力极低的相互作用和瞬时反应的研究。
因此,应用SPR生物传感器定量的研究结合反应几乎没有误差。
与其他生物物理的方法比较,如热量滴定的方法,SPR生物传感器具有需要的样品量少(一般只需要几微克)和自动化程度高(目前的技术可分析100~400个样品/天)的优点。
法布里珀罗基模共振英文The Fabryperot ResonanceOptics, the study of light and its properties, has been a subject of fascination for scientists and researchers for centuries. One of the fundamental phenomena in optics is the Fabry-Perot resonance, named after the French physicists Charles Fabry and Alfred Perot, who first described it in the late 19th century. This resonance effect has numerous applications in various fields, ranging from telecommunications to quantum physics, and its understanding is crucial in the development of advanced optical technologies.The Fabry-Perot resonance occurs when light is reflected multiple times between two parallel, partially reflective surfaces, known as mirrors. This creates a standing wave pattern within the cavity formed by the mirrors, where the light waves interfere constructively and destructively to produce a series of sharp peaks and valleys in the transmitted and reflected light intensity. The specific wavelengths at which the constructive interference occurs are known as the resonant wavelengths of the Fabry-Perot cavity.The resonant wavelengths of a Fabry-Perot cavity are determined bythe distance between the mirrors, the refractive index of the material within the cavity, and the wavelength of the incident light. When the optical path length, which is the product of the refractive index and the physical distance between the mirrors, is an integer multiple of the wavelength of the incident light, the light waves interfere constructively, resulting in a high-intensity transmission through the cavity. Conversely, when the optical path length is not an integer multiple of the wavelength, the light waves interfere destructively, leading to a low-intensity transmission.The sharpness of the resonant peaks in a Fabry-Perot cavity is determined by the reflectivity of the mirrors. Highly reflective mirrors result in a higher finesse, which is a measure of the ratio of the spacing between the resonant peaks to their width. This high finesse allows for the creation of narrow-linewidth, high-resolution optical filters and laser cavities, which are essential components in various optical systems.One of the key applications of the Fabry-Perot resonance is in the field of optical telecommunications. Fiber-optic communication systems often utilize Fabry-Perot filters to select specific wavelength channels for data transmission, enabling the efficient use of the available bandwidth in fiber-optic networks. These filters can be tuned by adjusting the mirror separation or the refractive index of the cavity, allowing for dynamic wavelength selection andreconfiguration of the communication system.Another important application of the Fabry-Perot resonance is in the field of laser technology. Fabry-Perot cavities are commonly used as the optical resonator in various types of lasers, providing the necessary feedback to sustain the lasing process. The high finesse of the Fabry-Perot cavity allows for the generation of highly monochromatic and coherent light, which is crucial for applications such as spectroscopy, interferometry, and precision metrology.In the realm of quantum physics, the Fabry-Perot resonance plays a crucial role in the study of cavity quantum electrodynamics (cQED). In cQED, atoms or other quantum systems are placed inside a Fabry-Perot cavity, where the strong interaction between the atoms and the confined electromagnetic field can lead to the observation of fascinating quantum phenomena, such as the Purcell effect, vacuum Rabi oscillations, and the generation of nonclassical states of light.Furthermore, the Fabry-Perot resonance has found applications in the field of optical sensing, where it is used to detect small changes in physical parameters, such as displacement, pressure, or temperature. The high sensitivity and stability of Fabry-Perot interferometers make them valuable tools in various sensing and measurement applications, ranging from seismic monitoring to the detection of gravitational waves.The Fabry-Perot resonance is a fundamental concept in optics that has enabled the development of numerous advanced optical technologies. Its versatility and importance in various fields of science and engineering have made it a subject of continuous research and innovation. As the field of optics continues to advance, the Fabry-Perot resonance will undoubtedly play an increasingly crucial role in shaping the future of optical systems and applications.。
关于科学家性格的英语作文The Enigmatic Psyche of Scientists: Unveiling theTraits that Drive Discovery.Scientists, the architects of human knowledge and technological advancement, often embody a unique blend of traits that distinguish them from other professions. Their unwavering curiosity, meticulous observation, andrelentless pursuit of truth have shaped the course of human history. However, beneath the seemingly objective exterior lies a complex and enigmatic inner world that has intrigued researchers for centuries.Intellectual Inquisiveness: The Unquenchable Thirst for Knowledge.The defining characteristic of scientists is their insatiable thirst for knowledge. Driven by an unyielding desire to understand the natural world, they are constantly seeking to unravel its mysteries. This intellectualcuriosity manifests itself in a perpetual state of questioning, experimentation, and exploration. Scientists challenge conventional wisdom, embrace the unknown, and venture into uncharted territories of thought.Methodical Observation: The Precision of Science.Scientists rely heavily on meticulous observation as the foundation of their understanding. They approach the world with a keen eye for detail, carefully gathering data, analyzing patterns, and drawing conclusions. This methodical approach to inquiry ensures accuracy, objectivity, and a deep comprehension of the phenomena under investigation. Precision is paramount in thescientific realm, where even the slightest deviation from established methodologies can lead to misleading results.Analytical Reasoning: Unraveling Complexities.Scientists possess an extraordinary ability to analyze complex information and draw logical conclusions. They employ critical thinking skills to evaluate evidence,identify relationships, and formulate hypotheses. This analytical prowess allows them to dissect intricate problems, construct logical arguments, and make informed decisions based on empirical data.Openness to Evidence: Embracing the Truth.A hallmark of the scientific mindset is a willingness to challenge existing beliefs and embrace new evidence. Scientists are not bound by preconceived notions or personal biases. Instead, they remain open to revision and correction, recognizing that the pursuit of knowledge is an ongoing process. This openness to evidence ensures that scientific understanding evolves over time as new discoveries are made.Perseverance: The Indomitable Spirit of Discovery.Scientific endeavors often face obstacles and setbacks. However, true scientists are characterized by an unwavering perseverance in the face of adversity. They refuse to succumb to disappointment or obstacles, instead viewingchallenges as opportunities for learning and refinement. This indomitable spirit drives them to persist in their research, even when the path forward seems uncertain.Collaboration and Communication: Sharing Knowledge.While scientists are often portrayed as solitary figures, collaboration and communication are essential to their work. They share ideas, findings, and resources with colleagues in their field. By uniting diverse perspectives and expertise, scientists accelerate the pace of discovery and ensure that knowledge is disseminated widely. Effective communication of their findings allows scientists to educate the public, inform policy decisions, and inspire future generations.Ethical Considerations: The Responsibility of the Scientist.As we delve deeper into the realm of scientific possibilities, it becomes imperative to consider theethical implications of our discoveries. Scientists have aresponsibility to ensure that their research is conducted responsibly and in a manner that benefits humanity. They must be mindful of the potential risks and consequences of their work, and they must adhere to ethical guidelines that protect the environment, human subjects, and society as a whole.The Elusive Balance: Personality and Passion.While the traits discussed above are generally associated with scientists, it is important to recognizethat scientists are as diverse as the fields they study. They come from different backgrounds, have unique personalities, and pursue a wide range of research interests. This diversity enriches the scientific community and fosters a continuous exchange of ideas and perspectives.In conclusion, the personality of scientists is a fascinating and complex subject that has been the focus of much research. Their insatiable curiosity, meticulous observation, analytical reasoning, openness to evidence, perseverance, collaboration, adherence to ethicalconsiderations, and diverse backgrounds contribute to their remarkable ability to push the boundaries of human knowledge and shape the world around us. As we continue to unravel the mysteries of the natural world, let us not only appreciate the discoveries made by scientists but also the enigmatic individuals who dedicate their lives to the pursuit of truth.。
毛额市鹌鹑阳光实验学校核心素养测评二十一Unit 2 Fit for life(限时35分钟)Ⅰ. 阅读理解AChina’s health and education authorities on Monday required the country’s schools to establish systems to report HIV/AIDS infection cases in students.The National Health and Family Planning Commission and the Ministry of Education said in a joint circular that infections of the virus among young students saw a marked rise in recent years, a sign of problems in education and students’ awareness about HI V/AIDS prevention.The two authorities required local health and education agencies to improve awareness at schools and called for establishment of an infection reporting system and regular consultation mechanism to analyze the cause of infections and formulate countermeasures.The circular also urged proper protection of infected students’ privacy.“Students in secondary schools and older should attend special classes on HIV/AIDS prevention focused on prevention of unsafe behavior as well as sexual resp onsibility and morality, ” the circular said.It also pledged financial support for volunteer services. The circular said HIV/AIDS voluntary counselling and testing services should be improved and schools should encourage students with risky behavior to use the services.【文章大意】这是一篇说明文。
面部识别技术对人类的影响英语作文The Impact of Facial Recognition Technology on Humanity.Facial recognition technology, a subset of biometric identification, has gained immense popularity in recent years, thanks to its accuracy, convenience, and widespread applicability. Its use cases range from unlocking smartphones to identifying criminal suspects in law enforcement agencies. However, this rapid advancement in technology has also sparked debates about its ethical implications and potential misuse. In this article, we will explore the pros and cons of facial recognition technology and its overall impact on humanity.Convenience and Security.The most obvious benefit of facial recognition technology is its convenience. Gone are the days when wehad to remember complex passwords or fumble with physical keys. With a simple scan of our faces, we can unlock ourphones, access secure areas, or even make payments. This ease of use has made our lives significantly more convenient.Moreover, facial recognition has also增强了安全性。
2006年第25卷第lo期传感器与微系统(Tr粕sducer蛐dMicmsystemII锄hnologies)57利用图像SPR分析仪检测多组分蛋白芯片母梁金庆,崔大付,蔡浩原,王军波,王于杰(中国科学院电子学研究所,传感技术国家重点实验室。
北京lO∞舳)摘要:介绍了一种颏型高通量表面等离子体谐振(sPR)生化分析仪。
基于图像分析技术和自动进样技术,该仪器可对微阵列sPR敏感芯片进行动态检测,实现高通量、多参数、多组分快速检测和定量分析。
对兔增G和人IgG分别与羊抗兔IgG和羊抗人lgG的免疫结合反应进行了实验检测。
结果表明:该分析系统具有灵敏度高、免标记等优点,且阵列芯片中所设置的参比单元可以消除溶液本体折射率和温度变化的影响,提高了测量精度和准确性;另外,芯片可以再生重复使用,降低了测试成本。
关键词:表面等离子体谐振;高通量;多通道;微阵列中圈分类号:TP206.1文献标识码:B文章编号:1000—9787(2006)lO一0057—03MllIti-anaIyteproteinchipdetectionbyimagiI唱SPR龇mIy阳r+LIANGJin—qing,CUIDa-fu,CAIHao-yuan,WANGJun·bo,WANGYu-jie(StateKey№聃伽ryofse衄坩n!chnolo醪,Ill鲥tIlteof硼∞衄瞄璐,Chi懈eA伪d蜘咿0fSd蚰嘲,B叫I哩10∞胁,Chi聃)Abtract:Ane、1rhigh—t}Iroughoutsurf∞epl鹊monreson如ce(SPR)bioche“calanal”icalin8tmmemi8reported.B鹪edSPRbi∞ensor叫nciple,coHlbinedwinlimageprocessiIIgtechnolo盱锄d蛐tos觚lplermb甜ca珊¥,tlleinstmmentc册mollitortllebiochemical聆actionkineticsoccurred砒tllesudhceofmicm-arrj盯SPRbiosensor,ermblinghi出-也姒lghput,烈lhi-锄母ef融detection.TherabhitIgG傩goat—anti·rabbitIgG∞dhum明IgG埘goat-明ti-hum明IgGi咖unoassay8地detected.哺ereslll协showmat访tlltIlerefe阳肿eceuintlIetIlicro·棚TlIys,Tloisesinducedbythebulkref}activeindex柚dfluctuationof枷bienttemperaturec明beeliminated。
硫化聚丙烯腈凝胶的制备及其锂硫电池性能赵永男1,2,李杨1,2,高海燕1,2,3(1.天津工业大学天津市先进纤维与储能技术重点实验室,天津300387;2.天津工业大学材料科学与工程学院,天津300387;3.南开大学先进能源材料化学教育部重点实验室,天津300071)摘要:为了提高硫化聚丙烯腈(SPAN )正极材料的电化学性能,以二甲基亚砜为溶剂,以去离子水为非溶剂,采用热致相分离法制备了聚丙烯腈凝胶(PAN g ),然后引入水合肼使PANg 的分子链间发生N —N 交联,制备出化学交联的聚丙烯腈凝胶(PAN g+N ),最终通过高温载硫法制得了硫化聚丙烯腈凝胶(SPAN g+N )。
利用SEM 、TEM 、FTIR 、XRD 等方法研究了材料的结构和物相特征。
结果表明:交联结构的SPAN g+N 具有高度互联的三维网络结构,结构稳定性及电化学性能均得到显著提高。
在0.5C 的电流密度时,首圈放电比容量为1219.8mA ·h/g ,循环100圈后的比容量为1000.6mA ·h/g ,容量保持率达到82.03%,平均每圈衰减率为0.18%。
关键词:锂硫电池;硫化聚丙烯腈;凝胶;热致相分离法中图分类号:TQ152;TQ427.26文献标志码:A 文章编号:员远苑员原园圆源载(圆园23)园6原园园67原07收稿日期:2022-03-02基金项目:国家自然科学基金资助项目(21703152);天津市青年科学基金资助项目(17JCQNJC06100);南开大学先进能源材料化学教育部重点实验室开放基金项目通信作者:赵永男(1970—),男,教授,博士生导师,主要研究方向为储能纳米材料。
E-mail :**************Preparation and lithium sulfur battery performance of sulfurizedpolyacrylonitrile gelZHAO Yongnan 1,2,LI Yang 1,2,GAO Haiyan 1,2,3(1.Tianjin Key Laboratory of Advanced Fibers and Energy Storage Technology ,Tiangong University ,Tianjin 300387,China ;2.School of Material Science and Engineering ,Tiangong University ,Tianjin 300387,China ;3.Key Laboratory of Advanced Energy Materials Chemistry ,Ministry of Education ,Nankai University ,Tianjin 300071,China )Abstract :In order to improve the electrochemical performances of sulfurized polyacrylonitrile 渊SPAN冤cathode material袁polyacrylonitrile gel 渊dennoted as PANg冤was prepared via thermally induced phase separation method using dimethyl sulfoxide as the solvent and deionized water as non -solvent.The introduction of hydrazine hydrate caused N要N crosslinking between the molecular chains of PANg to produce a chemically crosslinked polyacry鄄lonitrile gel 渊dennoted as PAN g+N 冤.The vulcanized polyacrylonitrile gel 渊dennoted as SPAN g+N 冤was obtained by high -temperature sulfurization method.The structure and phase characteristics of materials were studied bySEM袁TEM袁FTIR袁XRD and other methods.The results show that SPAN g +N with crosslinked structure has ahighly interconnected three -dimensional network structure with significantly improved structural stability and electrochemical properties.At a current density of 0.5C袁the discharge specific capacity of the material is 1219.8mA ·h/g at the first turn袁and it can still maintain 1000.6mA ·h/g after 100cycles袁with a capacity re鄄tention rate of 82.03%and an average attenuation of 0.18%per cycle.Key words :lithium-sulfur battery曰vulcanized polyacrylonitrile曰gel曰thermally induced phase separation锂硫电池不仅具有高理论容量(1675mA ·h /g )和高能量密度(2600W ·h/kg ),还具有低成本、环境友好等优点,成为备受关注的新一代电源。
the gentle power of highlysensitive people"Highly sensitive people" is a term used to describe individuals who have a heightened sensitivity to external stimuli, both positive and negative. This sensitivity can manifest in various ways, such as being easily overwhelmed by noise, crowds, or strong emotions. While this trait can sometimes be seen as a weakness, it also has many strengths and benefits.One of the greatest strengths of highly sensitive people is their ability to empathize with others. Because they are so in tune with their own emotions, they are often able to pick up on the emotions of those around them. This makes them great listeners and supporters, as they are able to understand and validate the feelings of others.Highly sensitive people also tend to have a great deal of creativity and imagination. Because they are so attuned to the world around them, they are able to see things from different perspectives and come up with unique solutions to problems. This creativity can manifest in various forms, such as art, music, writing, or entrepreneurship.In addition, highly sensitive people tend to be very introspective and self-aware. They are able to reflect on their own emotions and behaviors, and use this awareness to grow and develop as individuals. This self-awareness also allows them to be more in tune with their own needs and boundaries, which can help them to avoid burnout and maintain their mental and physical health.Overall, the gentle power of highly sensitive people is a strength that should be celebrated and embraced. While their sensitivity can sometimes be a challenge, it also allows them to connect with others on a deeper level, be creative and imaginative, and develop a greater sense of self-awareness.。
人面识别英语作文高一Title: The Ethical Implications of Facial Recognition Technology。
Facial recognition technology has become increasingly prevalent in our society, raising significant ethical concerns. While it offers various benefits in security, convenience, and efficiency, it also poses threats to privacy, civil liberties, and potentially perpetuates biases. This essay will delve into the ethical implications of facial recognition technology, exploring both its advantages and drawbacks.Firstly, facial recognition technology enhancessecurity measures by accurately identifying individuals in various contexts, such as airports, banks, and government facilities. It aids law enforcement agencies in identifying suspects or missing persons, thus improving public safety. For instance, surveillance cameras equipped with facial recognition software can swiftly track and apprehendcriminals, potentially preventing crimes and protecting citizens.Moreover, facial recognition technology streamlines processes in various industries, contributing to greater efficiency and convenience. For instance, it facilitates seamless authentication for accessing smartphones, making transactions, and unlocking doors. Additionally, it enables personalized marketing strategies by analyzing customers' facial expressions and reactions, thereby improving user experiences and boosting sales.However, despite its benefits, facial recognition technology raises significant ethical concerns, primarily regarding privacy and surveillance. The widespread deployment of facial recognition systems in public spaces threatens individuals' right to privacy, as it enables constant monitoring and tracking without their consent. This constant surveillance infringes upon personal freedoms and fosters a culture of surveillance, reminiscent of Orwellian dystopias.Furthermore, facial recognition technology is not infallible and is prone to errors, especially concerning accuracy and bias. Studies have shown that facial recognition algorithms often exhibit racial and gender biases, leading to misidentifications and wrongful arrests, particularly of minority groups. This exacerbates existing social injustices and perpetuates discrimination within law enforcement and other sectors where facial recognition is utilized.Another ethical concern is the lack of transparency and accountability in the development and deployment of facial recognition technology. Companies and government agencies often operate with minimal oversight, raising questions about data security, algorithmic transparency, andpotential misuse of collected data. The absence of clear regulations and standards exacerbates these concerns, leaving individuals vulnerable to exploitation and abuse.In response to these ethical dilemmas, it is imperative to implement robust regulations and guidelines governing the use of facial recognition technology. These regulationsshould prioritize transparency, accountability, and the protection of individuals' privacy rights. Additionally, there is a need for ongoing research and development to improve the accuracy and fairness of facial recognition algorithms, mitigating biases and ensuring equal treatment for all individuals.Furthermore, stakeholders, including governments, corporations, and civil society organizations, must engage in meaningful dialogue to address the ethical implications of facial recognition technology collaboratively. This dialogue should involve diverse perspectives and prioritize the interests of marginalized communitiesdisproportionately affected by facial recognition technology's negative consequences.In conclusion, facial recognition technology presents both opportunities and challenges, requiring careful consideration of its ethical implications. While it offers benefits in security and efficiency, it also raises concerns about privacy, bias, and accountability. By implementing robust regulations, fostering transparency,and promoting dialogue among stakeholders, we can harness the benefits of facial recognition technology while safeguarding individuals' rights and dignity in the digital age.。
In recent years,the field of robotics has seen a significant advancement,with the development of robots designed to detect and combat viruses.These robots are not just a product of science fiction they are becoming an integral part of our fight against infectious diseases.Lets delve into the world of virusdetecting robots and explore how they are revolutionizing healthcare.The Need for VirusDetecting RobotsThe COVID19pandemic has highlighted the importance of quick and efficient virus detection.Traditional methods of virus detection,such as PCR tests and antigen tests, require human intervention,which can be timeconsuming and prone to errors. Virusdetecting robots can automate this process,reducing the risk of human error and speeding up the detection process.Types of VirusDetecting Robots1.Automated Testing Robots:These robots are designed to handle the entire process of testing,from sample collection to analysis.They can perform tasks such as swabbing, pipetting,and analyzing samples,all without human intervention.2.Sanitization Robots:While not directly involved in virus detection,these robots play a crucial role in preventing the spread of viruses by disinfecting surfaces in public spaces. They use UVC light or chemical disinfectants to kill viruses on contact.3.DroneBased Surveillance:Drones equipped with sensors can monitor public spaces for signs of virus transmission.They can detect high concentrations of viral particles in the air,alerting authorities to potential hotspots.4.AIPowered Diagnostic Robots:These robots use artificial intelligence to analyze medical images,such as CT scans and Xrays,to detect signs of viral infections.They can assist doctors in making a quick and accurate diagnosis.Technologies Used in VirusDetecting RobotsMachine Learning Algorithms:These algorithms allow robots to learn from data and improve their detection capabilities over time.Swarm Intelligence:Some robots work in groups,using swarm intelligence to cover larger areas and detect viruses more efficiently.BioSensing Technology:Advanced sensors can detect the presence of viral particles in the air or on surfaces.Benefits of VirusDetecting RobotsSpeed:Robots can perform tests and analyses much faster than humans,leading to quicker results.Accuracy:Automated processes reduce the risk of human error,leading to more reliable results.Safety:Robots can work in environments that are too dangerous for humans,such as areas with high viral loads.CostEffectiveness:Over time,the use of robots can reduce the cost of virus detection and management.Challenges and Ethical ConsiderationsWhile virusdetecting robots offer numerous benefits,they also present challenges.These include the high initial cost of development and deployment,the need for regular maintenance,and the potential for job displacement in the healthcare sector.Additionally, ethical considerations must be addressed,such as data privacy and the potential misuse of technology.Future ProspectsAs technology continues to advance,we can expect virusdetecting robots to become more sophisticated and versatile.They may eventually be able to detect a wide range of viruses and even predict outbreaks based on patterns in data.The integration of robotics into healthcare is set to transform the way we respond to infectious diseases,making our societies more resilient in the face of pandemics.In conclusion,virusdetecting robots represent a significant step forward in our ability to combat infectious diseases.They offer a blend of speed,accuracy,and safety that is unmatched by traditional methods.As we continue to refine this technology,we can look forward to a future where virus detection and management are more efficient and effective than ever before.。
Analytica Chimica Acta 759 (2013) 105–109Contents lists available at SciVerse ScienceDirectAnalytica ChimicaActaj o u r n a l h o m e p a g e :w w w.e l s e v i e r.c o m /l o c a t e /a caHighly sensitive detection of human cardiac myoglobin using areverse sandwich immunoassay with a gold nanoparticle-enhanced surface plasmon resonance biosensorOksana V.Gnedenko ∗,Yury V.Mezentsev,Andrey A.Molnar,Andrey V.Lisitsa,Alexis S.Ivanov,Alexander I.ArchakovIBMC RAMS,Pogodinskaya Street,10,119121Moscow,Russian Federationh i g h l i g h t sA highly sensitive immunoassay for detection of cardiac myoglobin was designed.A gold nanoparticles were used for SPR signal amplification.The limit of detection of cMb in a serum sample was found to be as low as 10pM.g r a p h i c a la b s t r a cta r t i c l ei n f oArticle history:Received 19April 2012Received in revised form 3October 2012Accepted 28October 2012Available online 12 November 2012Keywords:Surface plasmon resonanceReverse sandwich immunoassay Cardiac myoglobin Gold nanoparticles Signal amplificationa b s t r a c tA highly sensitive reverse sandwich immunoassay for the detection of human cardiac myoglobin (cMb)in serum was designed utilizing a gold nanoparticle (AuNP)-enhanced surface plasmon resonance (SPR)biosensor.First,a monoclonal anti-cMb antibody (Mab1)was covalently immobilized on the sensor sur-face.AuNPs were covalently conjugated to the second monoclonal anti-cMb antibody (Mab2)to form an immuno-gold reagent (Mab2-AuNP).The reverse sandwich immunoassay consists of two steps:(1)mix-ing the serum sample with Mab2-AuNP and incubation for the formation of cMb/Mab2-AuNP complexes and (2)sample injection over the sensor surface and evaluation of the Mab1/cMb/Mab2-AuNP complex formation,with the subsequent calculation of the cMb concentration in the serum.The biosensor signal was amplified approximately 30-fold compared with the direct reaction of cMb with Mab1on the sensor surface.The limit of detection of cMb in a human blood serum sample was found to be as low as 10pM (approx.0.18ng mL −1),and the inter-assay coefficient of variation was less than 3%.Thus,the developed SPR-based reverse sandwich immunoassay has a sensitivity that is sufficient to measure cMb across a wide range of normal and pathological concentrations,allowing an adequate estimation of the disease severity and the monitoring of treatment.© 2012 Elsevier B.V. All rights reserved.1.IntroductionMyoglobin is a heme-containing oxygen-binding protein that is present in the cytoplasm of both cardiac and skeletal muscle cells.∗Corresponding author.Tel.:+74992467115;fax:+74992450857.E-mail address:oksana gnedenko@pochta.ru (O.V.Gnedenko).Cardiac myoglobin (cMb)is an early biomarker for diagnostics of acute myocardial infarction (AMI):due to its low molecular weight (17kDa),myoglobin is rapidly released into the circulation when muscle cells are damaged.Indeed,the serum concentration of cMb is elevated above the normal range as early as 1h after AMI [1]and correlates with the degree of the myocardial injury [2].Currently used in medical practice immunoassays for AMI,biomarkers in blood serum have relatively low accuracy and0003-2670/$–see front matter © 2012 Elsevier B.V. All rights reserved./10.1016/j.aca.2012.10.053106O.V.Gnedenko et al./Analytica Chimica Acta759 (2013) 105–109sensitivity[3–5],complicating diagnostics and making the mon-itoring of disease treatment impossible.Therefore,new,more accurate assays are intensively being developed.Surface plasmon resonance(SPR)biosensor technology is a very promising approach [6]because it has a higher precision and reproducibility in compar-ison with standard enzyme-linked immunosorbent assay(ELISA) [7–10].For example,a special comparative investigation has shown that the intra-assay coefficient of variation(CV,standard deviation/mean)and inter-assay CV for ELISA reached22%and 38%,respectively,whereas the corresponding CV values for SPR do not exceed0.4%and4%(see the“Comparison of Biacore&ELISA”at /gehcls images/GELS/Related%20 Content/Files/1314774443672/litdoc2891681320110831120514. pdf).The SPR analysis of protein concentration based on its direct reaction with an immobilized affinity ligand(for example,a mono-clonal antibody)has a detection limit of approximately1nM. However,the concentration level of AMI biomarkers in blood serum is low due to the extremely small damaged area of the heart mus-cle,and only a trace amount of protein biomarker is mixed with the large volume of circulating blood.It is for this reason that increasing the SPR biosensor sensitivity is highly important for the creation of the new SPR-based diagnostic methods.In recent years,several approaches to the amplification of an SPR signal have been reported,including a sandwich method with two antibod-ies[11–13],the usage of different nanoparticles[14–16]or latex particles[17],rolling-circle amplification[18]and enzymatic pre-cipitation[19–21].The application of gold nanoparticles(AuNPs) for SPR signal amplification seems the most promising approach, as the protein–colloidal gold complexes cause a higher refractive index than the protein or other biomolecules alone,and AuNP-enhanced SPR sandwich immunoassays were described[22–24]. AuNPs are usually used as strengthening agents that interact with an analyte previously captured on the sensor surface(direct sand-wich immunoassay).At thefirst step,the direct reaction of the analyte with thefirst monoclonal antibody(Mab1),immobilized on the sensor surface,occurs when the sample is injected through theflow cell of the biosensor.The SPR signal is then amplified by sandwich formation during the binding of AuNPs conjugated with the second antibody(Mab2)[22]or biotinylated antibody and streptavidin modified AuNPs[23].The main disadvantage of the direct sandwich assay is the long duration of thefirst phase(1h or more)of analyte binding on the chip with Mab1,which is due to the extremely low concentration of the analyte in the sample and the small surface area of the chip. Therefore,this stage is actually a bottleneck in the serial analyses of samples.The solution for this problem is to implement the analytefish-ing at thefirst phase during the preparation of the serum samples. The molecularfishing occurs on the surface of the Mab2-AuNPs added to the serum.This approach has two significant advantages: (i)the analyte binding occurs on a considerably larger surface of the AuNPs distributed throughout the sample volume and(ii)the process of molecularfishing can be executed in parallel using many samples of serum.As a result,the average assay time can be signifi-cantly reduced whereby the biosensor analysis becomes a one-step process of binding the target protein complex to Mab2-AuNPs on the sensor surface with immobilized Mab1.In fact,this approach corresponds to a reverse sandwich immunoassay.Analogous assay was developed previously for the quantification of human tissue inhibitor of metalloproteinases-2[24].The purpose of this study was to develop a highly sensitive reverse sandwich immunoassay for human cardiac myoglobin (cMb)using an AuNP-enhanced SPR biosensor.This assay con-sists of two steps:(1)mixing the serum sample with immunogold reagent(Mab2-AuNPs conjugate)and incubation for the formation of cMb/Mab2-AuNPs complexes and(2)sample injection over the biosensor surface and evaluation of the Mab1/cMb/Mab2-AuNPs complex formation,with the subsequent calculation of the cMb concentration.The biosensor signal was amplified approximately 30-fold in comparison with the direct reaction of cMb with Mab1 on the sensor surface.The limit of detection of cMb in a human blood serum sample was found to be as low as10pM(approx.0.18ng mL−1),and the inter-assay coefficient of variation(CV)was less than3%.The measurable range covered concentrations from 1pM to20nM cMb in human blood sera.2.Materials and methods2.1.ChemicalsThe following reagents for the Biacore optical biosensor were obtained from GE Healthcare(Russia):(1)HBS buffer(150mM NaCl,3mM EDTA,0.005%surfactant P20and10mM HEPES,pH 7.4);(2)10mM acetate buffer(pH5.0);(3)10mM glycine–HCl (pH2.5)and(4)an amine coupling kit containing EDC(1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride),NHS (N-hydroxysuccinimide)and ethanolamine–HCl(1M,pH8.5).The following materials were obtained from USBio(USA):(1) human cardiac myoglobin(cMb)(catalog no.M9800-18);(2)a matched pair of mouse anti-human cMb monoclonal antibodies Mab1(catalog no.M9800-16)and Mab2(catalog no.M9800-16A) and(3)human myoglobin-free serum(catalog no.S1005-14).Human myoglobin from skeletal muscle(sMb)was obtained from Serva(Germany)(catalog no.29895.01).Hydrogen tetrachloroaurate(HAuCl4),sodium borohydride and sodium citrate were purchased from Sigma–Aldrich.Other reagents were of analytical grade and obtained from local suppliers.2.2.SPR measurementsAll of the SPR experiments were performed using the Biacore 3000biosensor(GE Healthcare,USA).The instrument was operated using the“Biacore Control v.4.1.1”software,and the data were eval-uated using“Biacore Evaluation v.4.1”.HBS was used as the running buffer for the SPR assays.All of the binding assays were performed with Biacore CM3sensor chips containing a short carboxymethy-lated dextran matrix.The covalent immobilization of proteins(cMb,sMb,Mab1and Mab2)on the surface of the CM3chip was performed using the standard EDC/NHS amino-coupling Biacore protocol.In all of the experiments,theflow cell1(Fc1)without the immobilized protein was considered as the reference cell for the correction of the signal responses.After each cycle of SPR measurement,the sensing surface was regenerated by the injection of10mM glycine–HCl(pH2.5)for 0.5min at aflow rate of50L min−1.The sensing surface was sta-ble for more than30binding and regeneration cycles,thus enabling the re-use of the sensor chip.2.3.Preparation of antibody-coated gold nanoparticles (immunogold reagent)Gold nanoparticles were synthesized by reducing HAuCl4with sodium borohydride in the presence of sodium citrate[25].The prepared AuNPs were stored at4◦C in the dark.The antibody-coated AuNPs(Mab2-AuNPs)were prepared by mixing0.1mL Mab2solution(50g mL−1in5mM PBS,pH7.5) with0.9mL AuNPs suspension and incubating at room temper-ature for60min.AuNP-antibody complexes are formed due to the reaction of protein SH groups with gold and the formationO.V.Gnedenko et al./Analytica Chimica Acta759 (2013) 105–109107Fig.1.A schematic diagram of the AuNP-enhanced SPR reverse sandwich immunoassay.of Au–sulfur bonds.A blocking step was then performed by the addition of0.2mL BSA solution(10%[w/v]in50mM PBS,pH 9.0)and incubation at room temperature for20min.The Mab2-AuNPs were centrifuged at16,000×g for60min,and the pellet was re-suspended in HBS-buffer and stored at4◦C for up to 2weeks.2.4.Preparation of cMb standard solutions and calibrationsamples of serum with given cMb concentrationsStandard solutions of cMb from1pM to20nM were obtained by diluting the stock solution by HBS.The calibration samples of blood serum with given cMb concentrations from1pM to20nM were prepared by mix-ing the cMb stock solution with human myoglobin-free serum.2.5.Evaluation of SPR signal amplification by AuNPsFor the evaluation of the SPR signal amplification,we com-pared the interaction of three samples containing the same cMb concentration(4nM)with immobilized Mab1:(i)standard cMb solution in HBS;(ii)the complex cMb/Mab2and(iii)the complex cMb/Mab2-AuNPs.Both of the complexes were prepared by mix-ing equal volumes of the Mab2solution or Mab2-AuNPs suspension and standard cMb solution in HBS and incubating for1h at room temperature.All three of the samples were injected through the flow cell2(Fc2)with the immobilized Mab1and the controlflow cell(Fc1)for5min at aflow rate of5L min−1.2.6.Design of the AuNP-enhanced SPR reverse sandwich immunoassayFig.1shows a schematic diagram illustrating the steps of the AuNP-enhanced SPR reverse sandwich immunoassay.First,the immunogold reagent(conjugate Mab2-AuNPs)is added to the sam-ple of blood serum and incubated for1h at room temperature.The reaction between the serum cMb and immunogold reagent occurs with the formation of the primary complex cMb/Mab2-AuNPs. For serial analyses,this step can be performed simultaneously in a standard96-well plate.Because the Biacore3000biosensor is compatible with such plates,the second step of the assay can be performed automatically by serial injections of the samples from the plate.The serum mixture is injected through the biosensorflow cell (Fc2)with the immobilized Mab1and the controlflow cell(Fc1). The second reaction occurs with the formation of the sandwich Mab1/cMb/Mab2-AuNPs.3.Results and discussion3.1.Validation of antibody affinity and specificityTo assess the Mabs affinity and specificity,the interactions of Mab1and Mab2with two antigens(cMb and sMb)immobilized in different channels were analyzed in the range of the Mabs concen-trations from0.5nM to135nM.The obtained kinetics and equilibrium parameters for the antibody–antigen interactions are presented in Table1.Mab2inter-acted only with cMb,whereas Mab1interacted with both types108O.V.Gnedenko et al./Analytica Chimica Acta 759 (2013) 105–109Table 1Association (k on )and dissociation (k off )rate constants and equilibrium dissociation constants (K D )of the antigen–antibody complexes.Pairs k on ,M −1s −1k off ,s −1K D ,MMb im /Mab1(3.46±0.03)×105(3.36±0.07)×10−3(9.7±0.2)×10−9Mb im /Mab2(1.20±0.01)×105(5.7±0.5)×10−4(4.7±0.4)×10−9sMb im /Mab1(1.02±0.01)×105(2.97±0.03)×10−3(2.91±0.04)×10−8sMb im /Mab2No interaction Mab1im /Mb (4.36±0.07)×104(3.8±0.02)×10−3(8.7±0.1)×10−8Mab2im /Mb(2.02±0.07)×105(9.6±0.7)×10−4(4.8±0.4)×10−9of myoglobins (cMb and sMb).Consequently,Mab2antibodies arespecific and have higher affinities to cMb when compared to Mab1,which results from the difference in the dissociation rate constants of the cMb im /Mab1and cMb im /Mab2complexes.In addition,the K D value for the cMb/Mab2complex does not depend on which pro-tein was immobilized.Mab2was used for the preparation of the conjugate with AuNP.3.2.Evaluation of the SPR signal amplification by AuNPMab1was covalently immobilized on the surface of the CM3optical chip in flow cell Fc2at 5400RU (equal to 5.4ng of pro-tein).The interactions of free cMb,complex cMb/Mab2or complex cMb/Mab2-AuNPs with immobilized Mab1were analyzed (see the typical sensograms in Fig.2).Compared with the direct bind-ing reaction of cMb with immobilized Mab1(curve 1),complex cMb/Mab2(curve 2)and complex cMb/Mab2-AuNPs (curve 3)amplified the biosensor signal by approximately 5-and 30-fold,respectively.It appears that the enhancement of the biosensor response using the Mab2antibodies is not as substantial as with the conjugates of these antibodies with nanoparticles.3.3.Implementation of AuNP-enhanced reverse sandwich SPR immunoassaysA number of sensograms for the calibration samples of blood sera with the given cMb concentrations (from 1pM to 20nM)was obtained for the direct and AuNP-enhanced reverse sandwich SPR immunoassays.The dependence of the biosensor signal on thecMbFig.2.Typical sensograms of the cMb (4nM)interaction with immobilized Mab1on the CM3chip:free cMb (1),complex cMb/Mab2(2)and complex cMb/Mab2-AuNP(3).Fig.3.Dependence of the biosensor signal on the cMb concentration in blood serum (calibration samples)in the direct SPR immunoassay (- -)and in the AuNP-enhanced reverse sandwich SPR immunoassay (- -).concentration in the calibration blood serum are shown in Fig.3:the minimal detectable concentration of cMb decreased significantly in the case of the reverse sandwich immunoassay.In comparison with the direct assay based on the reaction of free cMb with immo-bilized Mab1,the reverse sandwich immunoassay exhibits a higher sensitivity due to the amplification of the biosensor signal.The minimal detectable concentrations of cMb were deter-mined with commonly used statistical analysis.Detection limit was defined as the smallest concentration of analyte that can be differentiated from zero with confidence.It was defined as the concentration of analyte that corresponds to a signal that is two standard deviations above the mean signal derived from multiple determinations of a sample free of analyte.The minimal concen-tration of biomarker that has been detected in the direct biosensor analysis was 4nM (approx.70ng mL −1),whereas this value was 10pM (approx.0.18ng mL −1)for the gold nanoparticle–antibody conjugate.Thus,the utilization of the conjugate resulted in a decrease of the limit of the minimal detected biomarker concen-tration by more than 2orders.This detection result is comparable with other NP-enhanced SPR assays [23,26,27]and is better than the results for an electrochemical nanobiosensor [28].At the same time,assay described in [24]allows to detect 0.5pM of human tis-sue inhibitor of metalloproteinases-2.However,inactive form of matrix metalloproteinase-2and monoclonal antibody to inhibitor were used as affine reagents.This assay is difficult adaptable for the analysis of other proteins.In our case,two antibodies inter-acting with two epitopes of the protein were used.Therefore,our approach is more universal for the analysis of any protein which has a suitable pair of antibodies.3.4.Determination of intra-and inter-assay coefficients of variation (CVs)The precision of the designed assay was estimated by cal-culating the intra-assay coefficient of variation (CV;standard deviation/mean)of multiple determinations of the same samples,all measured in a single batch.We have used multiple samples across the full range of measurable values and calculated the average intra-assay CV.The reliability of the designed assay was estimated by calculating the inter-assay CV (day-to-day variation)of multiple determinations of a single sample measured on differ-ent days.The average values of the intra-and inter-assay CVs were 0.6%and 2.8%,respectively.O.V.Gnedenko et al./Analytica Chimica Acta759 (2013) 105–1091094.ConclusionA highly sensitive quantitative analysis of protein biomarkers in human blood serum is very important for the diagnoses of various diseases and the monitoring of their treatment.The low accuracy of conventional immunoassay methods results in an inadequate assessment of the severity of the disease and,in particular,the effectiveness of the treatment.These limitations apply to all of the assays for human cardiac biomarkers(cardiac myoglobin,troponin I,troponin T and creatine kinase).In this study,we had developed a highly sensitive technology for the detection of human cardiac myoglobin in blood serum using a reverse sandwich immunoassay with AuNP-enhanced SPR biosens-ing for the purposes of diagnostics and the monitoring of heart attack treatment.The developed technique can be applied for the analysis of other protein biomarkers.Moreover,this technology can be used to ana-lyze multiple biomarkers simultaneously in one sample of blood serum using the mixture of several immunogold reagents,followed a separate analysis using different channels of the SPR biosensor with the corresponding immobilized Mab1.AcknowledgementsThis work was supported in part by RF State Contracts 02.512.11.2212and16.552.11.7001.The authors also thank GE Healthcare(Russia)for scientific and technical support. 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