健康大鼠经间歇性低氧训练后的心脏功能

脂联素对大鼠慢性间歇性低氧所致心肌重塑的影响朱选风;苏梅;丁文筱;丁宁;黄茂;张希龙【摘要】ObjectiveTo investigate the effects of chronic intermittent hypoxia (CIH) on myocardiac remodeling and intervention role of adiponectin(Ad) in the process.Methods A total of 45 Wistarrats were randomly divided into3 groups: normal control (NC) group, CIH group and CIH+Ad group (10µg Ad injection through caudal vein twice per week, for 5 weeks). After 35 days’ CIH exposure (in a chamber with 60 s nitrogen infusion to reduce theoxygen concentration to 5% followed by another 60 s oxygen infusion to increase theconcentration to 20% for totally 8 h of each day),Masson analysis was used to detect the left ventricular fibrosis, and Western blot was used to measure the protein expression of collagenⅠ, collagenⅢ a nd pathway proteins TGF-β/smad2/3. RT-PCR was used to study the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2).Results After CIH exposure, the left ventricular fibrosis was significantly more severe in CIH group than in NC groupand CIH+Ad group (P<0.05), and there was statistical difference between the 2 latter groups (P<0.05). In addition, the protein expression of collagenⅠ and collagenⅢ and the mRNA expression of MMP-2 and TIMP-2 were the highest in CIH group, the lowest in NC group, and CIH+Ad group in the middle. There was a significant difference among 3 groups (all P<0.05). The pathway proteins TGF-β/smad2/3 were most significantly expressed in CIH group (P<0.05) , but there was still significant differencein their expression between NC group and CIH+Ad group(P<0.05).Conclusion CIHmay cause may left ventricular remodeling, while Ad supplementmay ameliorate the impairmentvia inhibiting TGF-β/smad2/3 pathway.%目的：探讨慢性间歇性低氧（chronic intermittent hypoxia，CIH）对心肌重塑的影响及脂联素（adiponectin， Ad）的干预作用。

关于急、慢性缺氧对大鼠心功能的影响的问题龙超良 周智 尹昭云 孙兴斌 路新强 汪海龙超良 尹昭云 孙兴斌 军事医学科学院卫生学环境医学研究所，天津 300050周智 海军总医院心电图室，北京：100036路新强 汪海军事医学科学院毒物药物研究所，北京 100850关键词：心功能；缺氧；缺氧耐力；低氧适应航天医学与医学工程990408摘要：目的 研究急性缺氧和慢性适应性缺氧对大鼠心脏收缩和舒张功能的影响。

方法 间断低氧习服大鼠(3000m、5000m各2wk,4h/d)和正常大鼠经8000m缺氧4h后，观察其整体心功能。

结果 与常氧对照组相比，急性缺氧大鼠左心室反映收缩功能的指标LVSP, +dp/dt max,Vpm, V max及反映舒张功能的指标-dp/dt max均显著降低，经慢性适应性缺氧后，大鼠左心室舒缩功能的各项指标虽仍低于常氧对照组，但显著高于急性缺氧组。

结论 急性重度缺氧可显著抑制心功能，动物经低氧适应后，心脏功能明显改善，可减轻缺氧对心脏的损伤。

中图分类号：R852.11 文献标识码：A 文章编号：1002-0837(1999)04-0267-03Effects of Acute Hypoxia and Hypoxic Acclimatization on CardiacFunctions of RatsLONG Chao-liang, ZHOU Zhi, YIN Zhao-yun, SUN Xing-bin, LU Xin-qiang,WANG HaiAbstract：Objective To study the effects of acute hypoxia and intermittent hypoxic acclimatization on cardiac systolic and diastolic functions in rats. Method Cardiac functions were investigated after intermittent hypoxic acclimatizated (3000 m and 5000 m,2 wk respectively,4 h/d) and normoxic rats were exposed to hypoxia (8000 m) for 4 h. Result The systolic functional parameters such as LVSP, +dp/dt max,Vpm,V max and the diastolic functional parameter -dp/dt max of acute hypoxic rats were reduced significantly as compared with normoxic control rats. After hypoxic acclimatization, the left ventricular functions of rats were increased significantly as compared with those of acute hypoxic rats. But they were still lower than those of normoxic control rats. Conclusion Acute hypoxia can affect cardiac functions and hypoxic acclimatization can alleviate the hypoxic effects on cardiac functions.Key words：cardiac function;hypoxia;hypoxia tolerance;hypoxic acclimatizationAddress reprint requests to:LONG Chao-liang.Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China低氧作为一种应激因素，几乎能影响机体所有的功能活动。

《中国组织工程研究》Chinese Journal of Tissue Engineering Research文章编号:2095-4344(2018)09-01344-061344·研究原著·www.CRTER .org高宇平，1970年生，山西省临县人，汉族，2014年山西医科大学毕业，博士，主任医师，主要从事冠心病介入治疗、心血管疾病干细胞治疗研究。

通讯作者：高宇平，博士，主任医师，山西大医院心血管内科，山西省太原市 030032中图分类号:R394.2 文献标识码:B稿件接受：2017-10-20Gao Yu-ping, M.D., Chief physician, Department of Cardiovascular Medicine, Shanxi Dayi Hospital, Taiyuan 030032, Shanxi Province, ChinaCorresponding author: Gao Yu-ping, Department of Cardiovascular Medicine, Shanxi Dayi Hospital, Taiyuan 030032, Shanxi Province, China低氧诱导大鼠脂肪干细胞旁分泌对心肌的保护作用高宇平1，林媛媛1，李学文1，范春辉2，杨 帆3，郝大洁4，葛文佳5 (山西大医院，1心血管内科，2设备科，4介入治疗中心，5科教处，山西省太原市 030032；3太原市中心医院急诊科，山西省太原市 030009) DOI:10.3969/j.issn.2095-4344.0752 ORCID: 0000-0003-1768-6574(高宇平)文章快速阅读：文题释义：低氧培养：当细胞或器官中的氧气低于空气中的氧气水平时的培养状态。

人体内部不同部位的细胞所需的氧气水平是不一样的，不论哪一器官或组织所需要的氧气含量均低于空气中的氧气含量。

低氧训练对大鼠Ｒｅｔｉｃ、ＲＢＣ和ＨＧＢ的影响作者：刘文锋郑澜来源：《首都体育学院学报》2007年第06期摘要：为了探讨采用高住(氧浓度为11.3％相当于海拔高度5000m)低练对大鼠网织红细胞(Retieulocyte，Retie)、红细胞计数(Red blood ceils，RBC)、血红蛋白(Hemoglo－bin，HGB)等的影响。

将56只健康雄性SD大鼠随机分为7组，每天高住低练组置氧浓度为11.3％低氧舱中3小时，每周6天，运动持续时间为2周，并常氧下为跑台训练，运动负荷由18X30m／rain递增至28X60m／rain，坡度始终保持10％，最后得出：两周实验后，网织红细胞有非常显著性差异(P0.05)，结论：①结果显示低氧刺激了骨髓的追血功能，②通过高住低练可以提高机体的栽氧能力。

关键词：高住低练；雄性SD大鼠；网织红细胞，红细胞计数；血红蛋白中图分类号：G804.2 文章编号：1009－783X(2007)06－0073－03 文献标识码：A作者简介：刘文锋(1982)，男，湖南平江人，研究生，研究方向为运动与人体生理功能评价，郑澜，女，湖南长沙人，副教授，博士，研究方向为低氧运动的适应机制。

高原训练是指有目的、有计划地将运动员组织到具有适宜海拔高度的地区，进行定期的专项运动训练的方法，高原地区大气中的氧分压随海拔升高而降低，肺泡气氧分压和动脉血氧饱和度也随之降低，使人处于不同程度的缺氧。

在高原低压缺氧和训练负荷双重刺激，可以使训练者产生强烈的应激反应，调动体内的机能潜力，从而使机体产生一系列的抗缺氧的生理适应变化。

美国Levine博士在1991年首先提出的高住低练(Liv－ing high and training low，HiLo)的高原训练方法为最快提高耐力运动成绩，它就是让运动员居住高原或人工低氧环境，在平原或较低的地方训练的方法，在Levine博士提出后，立即引起了有关专家、学者们的注意，引起了体育界的关注，美国、荷兰、日本、俄罗斯、澳大利亚等国家很快在这方面大量投资，开始做基础研究，并转向实际的应用。

慢性间歇性低压低氧对大鼠心肌细胞内向整流钾通道的影响斯琴高娃;魏燕;宋胜利;周京京;杨晶;张翼【摘要】Objective To investigate the effect of chronic intermittent hypobaric hypoxia ( CIHH ) on inward rectifier potassium channel current ( Ikl ) in ventricular myocytes of rats. Methods Rats were experienced CIHH exposure in a hypobaric chamber, and whole cell patch - clamp was used to record Ikl in ventricular myocytes of rats. Results There was no significant difference of Ikl in ventricular myocytes between CIHH and control rats under the normal condition ( P > 0. 05 ). During simulated ischemia,Ikl was decreased ( P <0. 05 ),but the decreasing in 28 days CIHH treatment rats ( 20 ±25 )% ( n = 10 ) and in 42 days CIHH treatment rats ( 17 ±22)%( n = 10 ) was significantly smaller than that in contr ol rats (46 ±11 )% ( n = 10,P <0. 05), respectively. Conclusion CIHH can antagonize the inhibition of Ikl induced by simulated ischemia in ventricular myocytes effectively, which might be one of ionic mechanisms for CIHH antiarrhythmia in rats.%目的探讨慢性间歇性低压低氧(chronic intermittent hypobaric hypoxia,CIHH)对大鼠心室肌细胞内向整流钾通道电流(Ik1)的影响.方法通过低压氧舱制备CIHH大鼠模型;全细胞膜片钳方法记录大鼠心室肌细胞Ik1.结果基础条件下,CIHH与对照大鼠心肌细胞Ik1差异无统计学意义(P>0.05);模拟缺血导致大鼠心肌细胞Ik1明显降低(P＜0.05),CIHH处理28d大鼠心室肌细胞Ik1降低达(20±25)%(n=10),CIHH处理42d大鼠心室肌细胞Ik1降低达(17±22)%(n=10),明显小于control大鼠心室肌细胞Ik1降低的(46±11)%(n=10,P＜0.05).结论 CIHH可有效对抗模拟缺血对大鼠心肌细胞Ik1的抑制,可能是CIHH抗心律失常作用的离子机制之一.【期刊名称】《河北医科大学学报》【年(卷),期】2012(033)007【总页数】4页(P745-748)【关键词】肌细胞,心脏;钾通道;大鼠【作者】斯琴高娃;魏燕;宋胜利;周京京;杨晶;张翼【作者单位】北京市公安部警卫局干休所医务室,北京,100161;河北医科大学基础医学院生理学教研室,河北,石家庄,050017;河北省保定市第一人民医院肾内科,河北,保定,071000;河北医科大学基础医学院生理学教研室,河北,石家庄,050017;河北医科大学基础医学院生理学教研室,河北,石家庄,050017;河北医科大学基础医学院生理学教研室,河北,石家庄,050017【正文语种】中文【中图分类】R542.2慢性间歇性低压低氧（chronic intermittent hypobaric hypoxia，CIHH）具有明显的心脏保护作用，可对抗缺血/缺氧对心脏的损伤及心律失常［1-3］。

低氧训练大鼠心肌组织低氧诱导因子对血管新生的影响邹志兵;郑澜【期刊名称】《武汉体育学院学报》【年(卷),期】2010(44)10【摘要】目的:通过研究低氧训练大鼠心肌组织中HIF-lα和血管内皮CD34的蛋白表达情况,来初步探讨HIF-lα在促进心肌组织血管形成中的作用.方法:将健康雄性SD大鼠60只,按体重随机分为6组,运动组采用10 周递增负荷跑台运动训练,每周训练6天,运动量由第1 周的速度为15m/min、持续时间为25min 递增至第10 周速度为28m/min、持续时间为50min,低练组每周二、四、六在相当于海拔1 500m 的低氧环境中训练,并且在低氧环境中居住,低氧程度由第1 周相当于海拔1 800m 递增至第10 周相当于海拔3 600m.应用免疫组织化学、显微图象对HIF-1和CD34的阳性表达进行定性和定量分析.结果:低氧状态下,HIF-lα有大量的蛋白表达,低氧复合运动,表达更多,而CD34 蛋白表达只发生在常氧运动组和低氧训练组.结论: HIF-lα是促进心肌组织血管新生的一种重要因子,但须结合运动才能产生积极的作用.【总页数】6页(P67-72)【作者】邹志兵;郑澜【作者单位】广西民族大学,相思湖学院,广西,南宁,530008;湖南师范大学,体育学院,湖南,长沙,410081【正文语种】中文【中图分类】G804.22【相关文献】1.低氧训练状态下大鼠心肌组织细胞凋亡与血管新生的表达 [J], 邹志兵2.低氧训练大鼠骨骼肌组织低氧诱导因子1对血管新生的影响 [J], 邹志兵;毛淑章;郑澜3.低氧训练大鼠骨骼肌组织低氧诱导因子1对血管新生的影响 [J], 邹志兵;毛淑章;郑澜4.低氧训练状态下大鼠心肌组织细胞凋亡与血管新生的表达 [J], 邹志兵;5.低氧训练对大鼠心肌组织微血管生成的影响 [J], 邹志兵;郑澜因版权原因，仅展示原文概要，查看原文内容请购买。

低氧运动对大鼠血清VEGF含量及心肌VEGF基因和蛋白表达的影响宋凯;丁宁炜【摘要】Objective:This study was to observe both effects of hypoxia and exercise on serum vascular endothelium growth factor(VEGF) content and myocardial VEGF gene and protein expressions.Methods:SD rats were randomly assigned to six groups: normoxia control group(NC),hypoxia control group(HC),normoxia low-intensity exercise group(NEL),hypoxia low-intensity exercise group(HEL),normoxia high-intensity exercisegroup(NEH) and hypoxia high-intensity exercise group(HEH).NC,NEL and NEH were living in normoxia.HC,HEL and HEH were exposed to hypoxia,the oxygen content was 14.1%.NEL and HEL exercise intensity were15.2m/min,NEH and HEH were 26.8m/min,the slope of treadmill was 10 degree,40min/day,6days/week.After experiment,blood and heart were get after 24h of last training.The content of VEGF was tested.The gene and protein expressions of VEGF were detected by RT-PCR and Western Blotting.Results The content of serum VEGF had descent trend with the deepen of hypoxia level.The result of myocardial VEGFmRNA and protein expressions had gradually increase trend with deepen of hypoxialevel.Conclusion(1) Hypoxia,exercise,hypoxia exercise all can make the content of serum VEGF decrease,at the same time the gene and protein expressions of VEGF of myocardial all increase,presume muscular tissue incept and utilize increase of circulation VEGF,decrease serum VEGFcontent;(2)The gene and protein expressions of VEGF had close relationship with the level of hypoxia stimulate;(3)The variety of content and expression of hypoxia relevant factor could be as the evaluate index of the body to the oxygen deficit adaptive ability.%目的：观察低氧和不同运动强度对大鼠血清血管内皮生长因子（VEGF）含量及心肌VEGF基因和蛋白表达的影响。