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参附注射液对大鼠移植肝脏缺血-再灌注损伤的保护作用陈波;赖星【期刊名称】《中国药业》【年(卷),期】2012(21)8【摘要】Objective To investigate the protective role and mechanism of Shenfu Injection(SF) in ischemia - reperfusion injury of transplanted liver in rats. Methods Male SD rats were divided into three groups: SF group, control group and ischemia - reperfusion injury (I - RI) group. The SF group was infused SF 2 mL/100 g by lumbar vein before getting donor liver. The control group was infused with equivalent 0. 9% sodium chloride injection without any treatment for I - RI group. The level of TNF - a, ALT and AST were detected at 0,60,180 min after liver transplantation. The histopathological damage degree of grafted liver was observed by light microscope. Results The degree of histopathological damage at 180 min after reperfusion in the SF group was lower than that in the control and the I - RI group. The levels of TNF-α, ALT and AST at 60,180 min after reperfusion were significantly decreased in the SF group than the control and I - RI groups (P＜0. 05). Conclusion SF has significant protective effect on transplanted liver in rats via reducing the expression of TNF - α%目的研究参附注射液对大鼠移植肝脏缺血-再灌注损伤的保护作用及其机制.方法将雄性SD大鼠分为参附组、对照组和肝脏缺血-再灌注损伤模型组.参附组在获取供肝前经腰静脉灌注参附注射液(2mL/100 g),对照组注入等量0.9%氯化钠注射液,模型组不予处理.光镜观察术后180 min肝脏病理学变化.分别于肝移植完成后0,60,180 min测定大鼠血清丙氨酸氨基转移酶、天门冬酸氨基转移酶和肿瘤坏死因子-α水平.结果再灌注180 min后,参附组病理损害程度较对照组和模型组轻;再灌注60 min和180 min后,参附组丙氨酸氨基转移酶、天门冬酸氨基转移酶和肿瘤坏死因子-α因子水平明显低于对照组和模型组(P<0.05).结论参附注射液通过降低炎性因子肿瘤坏死因子-α表达水平,对大鼠移植肝脏缺血-再灌注损伤有明显保护作用.【总页数】2页(P28-29)【作者】陈波;赖星【作者单位】重庆市第九人民医院普外二科,重庆,400700;四川省宜宾市第三人民医院普外一科,四川宜宾,466000【正文语种】中文【中图分类】R285.5;R286【相关文献】1.杀菌性/通透性增加蛋白对大鼠移植肝脏缺血再灌注损伤的保护作用研究 [J], 肖宏;孙科;陈先林;王征夏;张胜龙;田刚2.参附注射液对大鼠移植胰缺血/再灌注损伤的保护作用 [J], 刘小南;霍婷婷;王为忠;管文贤;陈冬利3.参附注射液对大鼠移植胰缺血再灌注损伤的保护作用和机制 [J], 刘小南;霍婷婷;王为忠;董光龙;张洪伟;陈冬利4.参附注射液对大鼠移植肝脏缺血再灌注损伤的保护作用 [J], 张义彬;涂兵;龚建平;刘长安;陈蓁臻;郝荣涛5.缺血预处理对大鼠移植肝脏缺血再灌注损伤的保护作用 [J], 涂兵;严律南;刘智敏因版权原因，仅展示原文概要，查看原文内容请购买。
·论著·小鼠肝脏应用解剖及标准肝部分切除模型的建立周健 郭志勇 鞠卫强 焦兴元 王小平 韩明 胡安斌 巫林伟 袁小鹏 马毅 朱晓峰 何晓顺 【摘要】 目的 观测C57BL/6J小鼠肝脏的解剖及建立70%、90%标准肝部分切除模型。
方法 60只成年SPF级C57BL/6J小鼠随机分3组。
A组小鼠称量体质量、全肝质量及各肝叶质量，计算全肝质量/体质量比、70%和90%肝比例。
采用直视下分步单纯丝线结扎肝脏解剖功能单位后再切除肝叶的方法，B组行70%肝切除；C组行90%肝切除。
观察术后动物生存情况。
结果 小鼠全肝质量为（1.325±0.029）g，体质量为（26.5±1.14）g，左外侧叶质量为（0.433±0.041）g，中叶为（0.411±0.035）g，右前叶为（0.140±0.011）g，右后叶为（0.175±0.015）g，尾状叶为（0.099±0.006）g，全肝质量/体质量比为（4.92±0.13）%。
肝左外侧叶与中叶之和约占全肝比重的70%；仅剩余尾状叶则切除肝比重约为90%。
B组和C组手术成功率为100%，B组术后1周动物存活率为100%，C组术后1 d存活率为30%（6/20），2 d存活率为0。
结论 本研究首次明确小鼠各肝叶比例。
采用直视下分步单纯丝线结扎肝脏解剖功能单位后再切除肝叶的方法，极易建立稳定、可靠的小鼠70%和90%标准肝部分切除模型。
【关键词】 部分肝切除；应用解剖；小鼠；模型；标准Application anatomy of the liver and establishment of standard partial hepatectomy model in miceZhou Jian, Guo Zhiyong, Ju Weiqiang, Jiao Xingyuan, Wang Xiaoping, Han Ming, Hu Anbin, Wu Linwei,Yuan Xiaopeng, Ma Yi, Zhu Xiaofeng, He Xiaoshun. Organ Transplantation Center, the First AffiliatedHospital of Sun Yat-sen University, Guangzhou 510080, ChinaCorresponding author: He Xiaoshun, Email: gdtrc@ 【Abstract】 Objective To observe anatomy of liver and establish 70% and 90% standard partialhepatectomy model in C57BL/6J mice. Methods Sixty adult mice with a C57BL/6J SPF stage wererandomly equably divided into three groups, group A, group B and group C. Weight of body, total liver, andevery single liver lobe were weighed in group A, ratio of total liver weight/body weight, and proportion of 70%and 90% liver was calculated, respectively. 70% partial hepatectomy in group B and 90% partial hepatectomyin group C were performed, using step by step visually ligaturing anatomic functional unit of liver by onlythreads. Results The weight of total liver, body, left lateral lobe, median lobe, right anterior lobe, rightposterior lobe and caudate lobe in mice were (1.325±0.029) g, (26.500±1.140) g, (0.433±0.041) g,(0.411±0.035) g, (0.140±0.011) g, (0.175±0.015) g, (0.099±0.006) g, respectively, the ratio of totalliver weight/body weight was (4.92±0.13)%, the weight of left lateral lobe and median lobe accounted for70% of the whole liver, and the weight of other parts except caudate lobe preserved was 90%. The successfuloperation rate in both group B and C was 100%. The survival rate was 100% one week after operation ingroup B, 30% (6/20) one day after operation in group C, respectively, and no survival in group C two daysafter operation. Conclusions This study ascertains the proportion of every liver lobe in mice for the firsttime. Using step by step visually ligaturing anatomic functional unit of liver by only threads, it is easy toestablish stable and credible 70% and 90% partial hepatectomy model in mice. 【Key words】 Partial hepatectomy; Application anatomy; Mice; Model; StandardDOI：10.3877/cma.j.issn.1674-0793.2014.02.005作者单位：510080 广州，中山大学附属第一医院器官移植中心通讯作者：何晓顺，Email：gdtrc@啮齿类动物的肝脏部分切除后有强大的再生能力，通常7~10d就能完成肝脏的重建[1-3]。
香港“换肝之父”做肝移植1000元全包
佚 名
【期刊名称】《肝博士》
【年(卷),期】2009(000)004
【摘 要】香港公立医疗系统为全港市民提供90％的医疗服务，所有服务的收费都
是象征性的。“即使是大手术，让有香港‘换肝之父’美誉的著名外科医生范上达
做肝移植，10天下来，也是1000元全包。换了去私家医院做，至少几百万。”
【总页数】1页(P41)
【正文语种】中 文
【中图分类】R657.3
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