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安捷伦科技实用解决方案通讯本期内容：OpenLAB LC 溶出度软件溶出度交流溶出杯直接加热 (DVH)自动化合规性引擎 (ACE)用于溶出度 UV 检测的光纤MQ 文档验证溶出度资质与 280-DS 更新DDG 在线会议12345678第 2 页继续OpenLAB LC 溶出度软件安捷伦 LC 溶出度软件是 OpenLAB 色谱数据系统中 ChemStation LC 版的扩充模块。
作为安捷伦 OpenLAB 分析软件套装的主要组成部分，LC 溶出度软件为可控环境下的分析工作提供了所有手段。
它提供了一个通用解决方案，采用企业内容管理 (ECM) 或数据存储软件，实现安全、集中的数据存储和管理。
这些灵活的解决方案正广泛运行于大型公司及较小规模的实验室。
现有的色谱方法可以很容易地整合到自动生成的分析序列中。
通用的参数集是运行自动分析和将溶出样品的序列数据转换为溶出度结果的基础。
这些溶出实验的设置与定量药物数据和溶出实验的测试条件有关。
此外，还要评估质量检查（如在设定时间点的上、下限）和认可标准的 Q 值。
这一通用方案使我们能够将相同的设置应用到不同的仪器上，以生成精确可靠的溶出度测试结果。
12OpenLAB：续其设置可依据药典方法进行，且适用于手动和全自动采集的溶出样品。
这些设置与经过验证的 LC 方法相结合，可方便地适用于使用不同仪器的不同实验室。
为满足分析要求，所有安捷伦 LC 色谱系列设备均可控制。
OpenLAB LC ChemStation软件提供了一种控制集成的 LC 系统中各种不同模块的手段。
而且，带 ISET 的新型 1290 Infinity LC 系统具有对不同设备（包括安捷伦 LC 模块和其它供应商仪器）的模拟功能，极大提高了系统的灵活性。
无需额外的验证步骤，即可将现有 LC 方法整合到我们的溶出度实验设置中。
新的系统可以实现更快速、更简便的溶出度分析。
使用内置的方法向导可轻松设置控制溶出样品分析的参数。
Lab Tech 全自动凝胶净化-定量浓缩系统的维护与保养尊敬的客户您好!感谢您选择了我公司的仪器, 全自动凝胶净化-定量浓缩系统有关的维护与保养的相关说明如下，请参阅，谢谢。
1、在使用ASF-800 自动进样、收集装置时，请您在使用过程中不要随便移动、变更样品盘上的样品架，因为该位置为我们的工程师再给您安装时以调整好了的，否则将会使进样针折断等事故发生。
在实验结束以后，请尽可能的对其做一些防尘措施，以便延长其使用的寿命。
2、对于仪器的凝胶净化（GPC）模块而言，务必要对所使用的流动相（乙酸乙酯：环己烷=1:1）溶液进行超声、过滤处理以后方可在仪器中运行，否则将会使仪器的放空滤片座堵塞，从而导致仪器的高压泵压力超限而无法进行后续的操作。
若出现此类情况，请将仪器的放空滤片座拆除下来，超声清洗后，在安装上使用。
3、在使用GPC时,请注意流动相对凝胶色谱柱的影响, 即，在选择溶剂时，应该选择与仪器、净化柱兼容的溶剂及样品溶解性相符的溶剂,对于柱体上标有流动相为乙酸乙酯：环己烷=1:1的凝胶色谱柱,流动相只能使用乙酸乙酯：环己烷=1:1,尤其是不能使用二氯甲烷作为它的流动相,否则将会对凝胶色谱柱的填料的膨胀系数造成影响,从而使得柱子不能够有效的使用。
另外，在较长时间内不使用GPC凝胶系统时，请您将凝胶色谱柱取下，用特有的聚四氟堵头将其两端封好，在下次使用时在安装上，然后以其所使用的流动相冲洗十分钟以上（使其基线达到平稳）后，在进行使用。
4、GPC操作的注意事项a、分离原理的局限需注意的是，任何方法都不是万能的。
凝胶净化的原理是依据分子尺寸的排阻，若杂质与目标物的分子量接近，GPC就无法分离。
b、流动相纯度标准方法中使用的GPC流动相为乙酸乙酯:环己烷 1:1的流动相，考虑到降低本底效应，建议客户使用农残级的试剂，若没有也需将分析级或色谱级的试剂进行重蒸净化后方能使用。
c、流动相的用量由于方法中的流速为5mL/min，如果实验样品的数量很多，应该一次性配好流动相充足的量，避免中途在往试剂瓶里添加。
18GREEN PACKAGING国际资讯材热密封处理后再进行加压加热杀菌处理的。
一般的加压加热杀菌是在121℃左右进行的，而DIC 准备了适用于PP 容器的易剥离密封膜，当温度达到125℃仍可进行杀菌处理，该薄膜的耐高温性能也因此得到了很高的评价。
一方面，食品供应商也在检讨，如何通过高温迅速的处理，来达到防止食品在加热后口感和香味变差的同时，保证杀菌效率的目的。
另一方面，加压加热杀菌的时候，容器因内部压强升高而破裂的情况也值得考虑。
此次，DIC 采用了独特的配合技术和基层化技术，延续了以往产品的易剥离性能，成功开发出了可在130℃下进行加压加热杀菌的高耐热性易开封包装薄膜。
该产品也同样适用于烯烃聚合物的美国食品医药局的规——FDACFR§177.1520。
在高龄化社会的多样化趋势以及独居人群增加的形势下，居家烹饪以及简易处理成了一种饮食需求，包装食品的市场近年来以每年2%的增长率在上升，预计今后有持续增长的倾向。
因此DIC 株式会社研发的这一产品意义重大，并已经在“2016东京国际包装展”展出。
赫斯基携创新型PET 瓶胚注塑解决方案亮相展赫斯基注塑系统公司（Husky）近日宣布，将在2016 K 上展示其多项PET 瓶胚注塑解决方案，包括专门用于展示其革命性多层阻隔技术的体验中心。
该技术系在2015年推出，在将其他包装材料转为PET 方面具有巨大潜力，同时可使包装更为美观和结实。
赫斯基的多层阻隔技术自推出以来，市场反响积极。
该技术基于公司行业领先的HyPET ® HPP5平台之上，将强大、高效且易于维护的系统与阻隔层精确给料功能完美地融合在一起。
此外，该技术通过减少所需阻隔材料的使用量提高可回收性，同时展示出将新型可回收阻隔材料应用于PET 包装的潜力。
赫斯基在K 展的体验中心将重点展示该技术如何支持针对各类应用不同阻隔的能力，包括奶制品、果汁、酱料、茶类和碳酸软饮料等。
同时还将展示多层阻隔热流道技术，这一技术采用共注塑方式，通过与先进的熔道系统以及增强型控制相结合，精确分配阻隔材料。
Power Your Titration Benchwith LabX®L a b o r a t o r y S o f t w a r e2C o n n e c t Y o u r L a bSingle SoftwareFor Many Laboratory InstrumentsThe new LabX ® from METTLER TOLEDO offers a great new possibility to con-nect your titrators, balances, density meters and refractometers, melting point instruments and Quantos systems all with one single software. This uniform software and interface means less training and also helps toincrease your efficiency. With a unique approach, LabX allows you to work from either the instrument or PC or both and if required, with complete compliance.Reducing redundant software for different instruments helps you save time and money spent on maintenance and support. Use LabX to increase the efficiency of your workflow and eliminate waste by using LabX for your pa-perless lab documentation. Inte-grate LabX with 3rd party software such as LIMS, ERP , etc. for a fully integrated process.LabX offers many tools to help you optimize your workflow in the laboratory and increase efficiency. Connect your METTLER TOLEDO instruments to LabX and benefit from simplified operation, efficient method editing with the graphical layout, fast sample series modi-fications and tailored reports.Less training also means less time and money spent educat-ing your workforce. LabX offers the same concept of use for all instruments. Beyond that, users will recognize similar workflow concepts throughout the instrument terminals for fast adaptation to new instruments and seamless integration.Cost EffectiveWorkflow Optimization Less Training3LabX offers you all the conve-nience of centrally managed software. With one database all users have access to the same data, which can be restricted to the appropriate access accord-ing to your user management. Create your SOP methods once and all the instruments from dif-ferent laboratories will always have access to the latest version.Central Management4Work from PC or Titrator or Both For Flexible OperationAll methods and series are stored in one database. Thus the meth-ods and sample series that you create in LabX or at the instru-ment are always available wher-ever you are. Beyond that, the ti-trator and workbench intelligently display solely those methods and sample series that can be run by the selected titrator model.Each instrument connected to LabX has its own workbench correlating to that instrument. It offers all the components needed to run your daily tasks and the tools to monitor your sample series and results. Use the sta-tistics view for quick and easy inspection of results to see if they are within established limits.LabX offers you full flexibility for the way you want to work. Either start your analysis at the instrument or from the PC and have constant access to both your current sample analysis and latest results. No matter where you are use LabX Mail to receive a tailored message with the latest results, when a sample changer is ready to test new samples or when results are out of specification.LabX anticipates the different demands of users and the way they like to work. Thus, LabX of-fers you full flexibility wherever you work. For example: start your sample series at the titrator and move to other daily tasks. Open up the workbench from your office and monitor the same series you previously started.Methods & Series WorkbenchFlexible Operation F u l l F l e x i b i l i t y5Have access to your results from the titrator or your PC. Use search folders to structure results from different analyses. LabX offers you a sample and a series based view of your results to match your needs. Each series offers the statistics tab for a quick and simple overview of the results. Use LabX to simplify sample changer operation with thetransparent and easy-to-manage sample series tables. Track the state of your series from any LabX connected PC in your laboratory. Alter your series by adding or inserting urgent samples while operating.Whether you are on the go or cur-rently not located next to your titra-tor or LabX network, use LabX Mail to have messages or results sent to you. Increase your efficiency by receiving tailored messages: e.g. “Results out of specifications” or “Ready for more samples”.Results Automation LabX Mail6Execute Your Daily TasksWith One Click®Use the Scheduler to automati-cally start tasks, e.g. begin Karl Fischer pretitrations just before coming into the lab for the day. Or schedule LabX to do the daily sensor calibration for you. Periodically take samples of production-line baths to observe the correct ion concentrations.Execute your tasks with One Click. Simply create a shortcut to methods, sample series, series sequences, tasks or manual op-erations, e.g. drain your beaker to waste, and have direct access to your most common analyses. All the shortcuts you create at the workbench are also displayed at the titrator and vice versa.Excellence Balances connected to LabX have a dedicated entry point for titration tasks that re-quire weighing. Simply pick the task and the balance will guide you through the samples. Start your titration and add more sample weights as you‘re test-ing for improved productivity.One Click is a well-recognized concept of METTLER TOLEDO instruments. LabX offers you the same concept and helps to easily distribute shortcuts for different users over various instruments. To facilitate your workflow, LabX offers the task scheduler and SmartCodes ™ to automate youranalyses. Use SmartSample ™ for secure and efficient titration weighing.O n e C l i c k A p p r o a c hSchedule TasksShortcuts Simple Weighing7Remove transcription and order-ing errors as well as improve effi-ciency with the new SmartSample RFID technology workflow. Identify and weigh your titration samples at an Excellence Analytical Bal-ance with the SmartSample kit. Enter the sample identification (ID) at the balance to make sure the ID and weight are properly as-signed to the physical sample. Fully automate the selection of the correct method and transcription of samples IDs with a barcode or SmartSample RFID tag. Thus, eliminate sample order errors by reading the sample information as you test it, as well as ensur-ing the right method is selected for each product. Use the auto-mated 1D and 2D barcode reader with InMotion ™ Autosamplers.SmartSample ™ SmartCodes ™8R e g u l a t o r y C o m p l i a n c eTraceable and CompliantEnsured with LabX®Work from the instrument or PC and LabX will ensure that you are fully compliant with regulatory standards such as FDA 21 CFR Part 11. Use the services offered from METTLER TOLEDO for software verification (IPac) or full validation of your system with the Validation Manuals I & II. A sophis-ticated user management, electronic signatures and release process for methods and reports are additional handy features of LabX that support your daily reporting.Centrally set up the user manage-ment with users and assign rights to roles. Instruments connected to LabX have the same user man-agement, i.e. use the same login at the instrument and PC, to offer full traceability whether you work at the instrument or in LabX. Enforce your own account poli-cies to meet company or regula-tory guidelines and standards.The LabX Initial Software verifica-tion Package (IPac) is a service product of METTLER TOLEDO to ensure successful software instal-lation and proper operation at the installation site. Use the IPac to meet your standards for quality management and documentation. IPac contents are:– Installation Qualification (IQ)– Operational Qualification (OQ)– General System Suitability TestFor software validation in ac-cordance with regulatory bodies such as FDA, METTLER TOLEDO offers onsite validation support with the Validation Manuals I and II. Validation Manual I of-fers the necessary documenta-tion for vendor qualification and Validation Manual II for the validation of the software including installation and opera-tional qualification (IQ & OQ).User ManagementIPac Validation Manual I and II9The Regulation option of LabX server provides all the necessary tools to meet the FDA regulation (21 CFR Part 11) for data ma-nagement and storage. All rele-vant actions taken at the instru-ment or PC are recorded in the audit trail of LabX for full traceabi-lity and flexibility, no matter where you work.To differentiate between devel-opment and the final version of methods, report templates and other objects can be approved and then released. Use the flexible views at the titrator and LabX to display only the released method for daily use or switch to the lat-est amended method for any further modifications needed.Define your electronic signa-ture policies according to your standards. For example, make sure that new methods follow your own review and approval process before they are released for your daily applications in the lab. Reviewed objects such as methods, results or reports, etc. are protected from any alteration.Regulatory Compliance Propose & ReleaseElectronic Signature10M o d u l a r A p p r o a c hBuild the SystemThat Perfectly Matches Your NeedsLabX ® offers a modular licensing approach to meet your needs today and in the future. Choose the starter pack that suits your requirements and add optional licenses to match your customized needs. Integrate additional instruments to your LabX system by simply activating the relevant instru-ment license key in LabX.Titration Starter PacksIncluded Optional11automatically into LabX creating tasks or even start the analyses directly. Import and export data directly in CSV and XML formats. Exporting and importing was never more powerful and able to meet your needs.Straight fingerprint reader. With the regulation option combined with the user management option, your system will be audit-ready at any time.system integration option allows bi-directional integration. Centrally start and control tasks directly from your ELN or LIMS. These 3rd party systems are able to send tasks directly to the METTLER TOLEDO instrument touchscreen terminal.freely customize reports with all details and options, such as charts, plots, and tables.* Only available with LabX Serverrights each user or role is given. Login to all your instruments and PCs on your LabX net-work with the same username and password or fingerprint via the LogStraight™ fingerprint reader.OptionsName DescriptionPart bX IPacLabX Initial Software verification Package (IPac) ensures successful software installation51710898LabX Validation Manual I All information necessary for the qualification of METTLER TOLEDO as the software supplier (vendor qualification)30003640LabX Validation Manual II Starterpack TitrationAll instructions and forms necessary for the validation of the software (IQ, OQ).30097758LabX ® Servicesfor efficient LabX system qualification and validation/LabXTitrationMultiparameter Analysis with One Click ®Today’s quality control and testing labs have a multitude of tests to be per-formed on a single sample and often have multiple samples to run at the same time. Combining tests with a multiparameter system can op-timize accuracy and reproducibility while saving valuable time and en-suring proper correlation of results to sample identification.Benefits of a Multiparameter Analysis System:• Efficient high sample throughput• Robust system layout and smart security checks for reliable unattended operation • Simple and ergonomic operation – pleasant to work with, short learning period • Optimized for fast, accurate and repeatable tests, and cleaning • Secure data handling thanks to our powerful LabX ® software• Onsite METTLER TOLEDO service installation, qualification and training See what METTLER TOLEDO can do to optimize your lab with a Multipa-rameter Analysis system: /titration-multiparameterFor more informationMettler-Toledo AG, Analytical CH-8603 Schwerzenbach, Schweiz Tel. +41 44 806 77 11Fax +41 44 806 72 40Subject to technical changes© 10/2013 Mettler-Toledo AG, 30100354Marketing Titration / MarCom AnalyticalQuality certificate. Development, production and testing according to ISO 9001.Environmental management system according to ISO 14001.“European conformity”. The CE conformity mark provides you with the assurance that our products comply with the EU directives.One Click is a Registered Trademark of METTLER TOLEDO in Switzerland, the European Union, Russia and bX is a Registered Trademark of METTLER TOLEDO inSwitzerland, USA, China, Germany and a further 13 countries.。
InfinityLab Pursuit XRs C8 LC columns with 10-30ml solvent *************(24小时)化学品安全技术说明书GHS product identifier 应急咨询电话（带值班时间）::供应商/ 制造商:安捷伦科技贸易（上海）有限公司中国（上海）外高桥自由贸易试验区英伦路412号（邮编:200131)电话号码： 800-820-3278传真号码: 0086 (21) 5048 2818InfinityLab Pursuit XRs C8 LC columns with 10-30ml solvent化学品的推荐用途和限制用途INF6010050X212, INF6010100X212, INF6010050X300部件号:物质用途:分析化学。
高效液相色谱法柱溶剂体积: 10 - 30 ml（毫升）INF6010050X212 Pursuit XRs C8, 21.2x50mm, 5um, 11mL solvent INF6010100X212 Pursuit XRs C8, 21.2x100mm, 5um, 22mL solvent INF6010050X300 Pursuit XRs C8, 30x50mm, 5um, 22mL solvent安全技术说明书根据 GB/ T 16483-2008 和 GB/ T 17519-2013本产品被视为一件物品。
该安全数据表是针对本物品中的密封物质或混合物而编写。
GHS化学品标识:InfinityLab Pursuit XRs C8 LC 色谱柱,含 10-30 mL 溶剂有关环境保护措施，请参阅第 12 节。
物质或混合物的分类根据 GB13690-2009 和 GB30000-2013在合理且符合使用说明的情况下使用本物品，不会存在健康风险。
该物品中的物质或混合物已密封。
仅在未按产品说明书使用或处理物品时，可能会存在潜在健康风险和安全隐患。
生活垃圾渗滤液膜浓缩液全量化处理工艺应用分析摘要:生活垃圾渗滤液是城市生活垃圾堆肥过程产生的一种高浓度有机液体废弃物。
其处理一直是环境保护领域研究的焦点之一。
本文通过分析生活垃圾渗滤液膜浓缩液全量化处理工艺的应用，探讨了该工艺在生活垃圾渗滤液处理中的重要性和优势。
研究结果表明，该工艺能够高效、经济地将生活垃圾渗滤液处理为无害化、可回收利用的资源。
因此，本文进一步提出了在实际应用中可能面临的困难和挑战，并对未来的研究方向进行了展望。
关键词:生活垃圾渗滤液；膜浓缩液；全量化处理；工艺应用引言随着城市化进程的不断加快和人口的持续增长，生活垃圾问题日益突显。
其中，生活垃圾渗滤液是城市生活垃圾处理中一种常见的废弃物之一。
该液态废弃物含有大量的有机污染物和营养成分，对环境造成严重污染。
因此，寻找一种高效、经济的处理工艺是解决生活垃圾渗滤液问题的重要任务。
本文旨在研究生活垃圾渗滤液膜浓缩液全量化处理工艺及其在实际应用中的分析。
1.研究背景1.1生活垃圾渗滤液的特点生活垃圾渗滤液是城市生活垃圾经过堆肥过程后所产生的一种高浓度有机液体废弃物。
其特点包括高含水量、高浓度有机污染物、营养成分丰富等。
由于含有大量的有机物质，生活垃圾渗滤液对环境造成严重污染。
处理过程中需要考虑适当去除水分并降低其有机物质的浓度，以实现无害化处理和资源回收利用。
同时，其复杂的组成使得处理工艺需要具备高效、经济的特点，才能有效解决环保问题。
1.2目前处理方法的不足目前处理生活垃圾渗滤液的方法存在以下不足：处理效果有限，难以彻底去除有机污染物；成本高昂，缺乏经济可行性；处理过程复杂，需要大量设备和专业技术支持；排放物处理问题未得到充分解决，对环境产生二次污染；缺乏统一规范和政策支持，导致缺乏标准化处理手段。
因此，需要进一步研究和改进处理方法，以提高处理效果、降低成本并符合环保要求。
2.生活垃圾渗滤液膜浓缩液全量化处理工艺2.1工艺原理生活垃圾渗滤液膜浓缩液全量化处理工艺的原理主要包括膜过滤、浓缩和分离三个步骤。
二、离心超滤管浓缩样品
2.1 浓缩样品
1、要浓缩的样品含盐量必须0.3M以上，含盐量低的样品须补加到0.3MNaCl。
2、在内管（附有3KD膜）中加入500µl样品，将内管套入外管。
3、对称放置离心管，14520rpm(即14000g)离心。
（离心5min约浓缩2倍，10min约4倍，15min约6倍，20min约8倍，30min约10倍）。
4、离心结束后，将内管倒置套在另一个干净的离心管呢，3880rpm离心min收集浓缩后的样品。
或者用移液器直接吸出内管中的样品。
2.2 浓缩管使用后的处理和保存
1、使用完的浓缩管，立即加入500µl含1MNaCl的缓冲液（此缓冲液与浓缩样品的缓冲液一致），8000rpm离心20min。
2、甩净内管中的盐溶液，将内管放入超纯水中浸泡1h。
3、内管加入超纯水500µl，8000rpm离心5min。
4、甩净内管中的水，加入500µl0.2MNaOH，8000rpm离心20min。
5、甩净内管中的NaOH溶液，用超纯水再8000rpm离心5min。
6、甩净内管中的水，放入含0.2-0.5‰NaN3的生理盐水中。
7、外管用自来水洗净后，用超纯水冲洗干净，晾干。
双效浓缩罐清洁验证方案题目双效浓缩罐清洁验证方案文件编号SC—JB—04—003—01 保管部门行政人力部制定审核批准制定日期审核日期批准日期双效浓缩罐清洁验证方案目录1.目的2.适用范围3.责任者4.内容4.1概述4.2.验证目的4.3验证小组成员和职责4.3.1验证小组成员4.3.2验证小组成员职责4.4验证方法4.5执行的清洁程序及相关执行文件4.6关键部位的确定4.7物理外观检查4.8微生物检测4.9残留量检测4.10验证结果评定与结论4.11验证周期双效浓缩罐清洁验证方案1.目的：制订详细、合理的验证方案，对双效浓缩罐清洁全过程进行验证，确保双效浓缩罐的清洁达到规定要求，以防止污染和交叉污染，生产出质量稳定、符合质量标准的产品。
2.适用范围：适用于双效浓缩罐的清洁验证。
3.责任者：验证小组成员对本方案的实施负责。
4.内容：4.1概述双效浓缩罐是浓缩物料的设备，为了保证产品质量，避免污染和交叉污染，对浓缩后的双效浓缩罐进行清洁是非常必要的，因此我们建立了《双效浓缩罐清洁操作规程》，并按此清洁操作规程对设备进行清洁和消毒，以除去设备内外表面可见和不可见物质。
清洁该设备后，以该设备内表面最难清洁部位、药品最难溶活性成分残留量及微生物数不超过预定的限量为指标，对《双效浓缩罐清洁操作规程》进行验证。
验证以每批清血八味片浓缩结束后，双效浓缩罐的清洁作为验证数据收集及评估单位，连续取样3批进行试验，并分析数据，综合整个验证过程，得出验证结论。
4.2验证目的采用物理、化学分析方法和微生物检测方法来确认按《双效浓缩罐清洁操作规程》对双效浓缩罐进行清洁后，该设备内表面最难清洁部位的药品最难溶活性成分残留量及微生物数不超过规定的限量，证明《双效浓缩清洁操作规程》的可行性和可靠性，以表明本清洁方法能够达到防止污染与交叉污染的目的，特制定本验证方案进行验证。
4.3验证小组成员及职责4.3.1验证小组成员4.3.2验证小组成员职责武治国负责起草清洁验证方案，组织协调验证工作，并总结验证结果，起草、整理验证报告。
二、离心超滤管浓缩样品2.1 浓缩样品1、要浓缩的样品含盐量必须0.3M以上，含盐量低的样品须补加到0.3MNaCl。
2、在内管（附有3KD膜）中加入500µl样品，将内管套入外管。
3、对称放置离心管，14520rpm(即14000g)离心。
（离心5min约浓缩2倍，10min约4倍，15min约6倍，20min约8倍，30min约10倍）。
4、离心结束后，将内管倒置套在另一个干净的离心管呢，3880rpm离心min收集浓缩后的样品。
或者用移液器直接吸出内管中的样品。
2.2 浓缩管使用后的处理和保存1、使用完的浓缩管，立即加入500µl含1MNaCl的缓冲液（此缓冲液与浓缩样品的缓冲液一致），8000rpm离心20min。
2、甩净内管中的盐溶液，将内管放入超纯水中浸泡1h。
3、内管加入超纯水500µl，8000rpm离心5min。
4、甩净内管中的水，加入500µl0.2MNaOH，8000rpm离心20min。
5、甩净内管中的NaOH溶液，用超纯水再8000rpm离心5min。
6、甩净内管中的水，放入含0.2-0.5‰NaN3的生理盐水中。
7、外管用自来水洗净后，用超纯水冲洗干净，晾干。
超滤管使用注意事项2012-06-06 18:12:28| 分类：默认分类| 标签：|字号大中小订阅蛋白浓缩和换Buffer通常使用的超滤管，常用Millipore的Amicon-Ultra-15超滤管（MWCO10kD）。
也有其它型号的、不同体积大小和MWCO超滤管可选，视目的蛋白的分子量与浓缩前体积、浓缩目标体积而定。
可重复使用，使用一次就扔掉太浪费。
以下是个人总结的使用方法和注意事项。
1、选择合适的超滤管，主要考虑MWCO和浓缩体积，最常见的是Ultra-15（10kD）。
到底选择多大截留分子量比较合适呢？10kDa、5kDa、还是30kDa？通常应截留分子量不应大于目的蛋白分子量的1/3，比如目的蛋白分子量为35kDa，就可以选择10kDa截留分子量的超滤管。
