下载文档原格式
兔急性肠道缺血再灌注损伤模型的建立【摘要】目的建立小肠急性缺血再灌注损伤模型，确定合适的肠系膜上动脉阻断时间。
方法将70只新西兰兔按不同的肠系膜缺血时间（0、15、30、45、60 min）分为A、B、C、D、E共5组，每组14只。
每组取8只于恢复血供2 h后留取兔下腔静脉血标本及肠组织，检测血清中丙二醛（MDA）含量的变化，光镜下观察小肠组织形态学变化并对小肠黏膜损伤程度进行评分。
另6只兔用于术后24、48、72 h 生存率的观察。
结果 A、B、C组的术后生存率均>83.3%。
C、D、E 组的MDA含量及肠黏膜损伤评分与A组比较，差异均有显著性（F=12.13、280.24,P<0.01）。
结论肠系膜缺血30 min再灌注2 h 是建立兔小肠急性缺血再灌注损伤的合适时间。
【关键词】肠；缺血；再灌注损伤；模型,动物［ABSTRACT］Objective To create a model of acute intestinal ischemia reperfusion injury in rabbits so as to determine suitable blocking duration of superior mesenteric arteries (SMA). Methods Seventy rabbits were divided into five groups as groups A, B, C, D, and E, based on the different blocking durations of SMA for 0, 15, 30, 45 and 60 minutes, respectively, with 14 rabbits in each group. Eight of the rabbits in each group were detected for serum levels of MDA 2 h after recovery of blood supply, the changes of histomorphology of small intestine observed light microscopically. The other sixrabbbits in each group were used to observe the 24 h, 48 h and 72 h survival rates after the blockage of SMA. Results The survival rates were over 83.3% in groups A, B and C. Serum levels of MDA and score of injury of intestinal mucosa were significantly higher in groups C, D and E than that in group A (F=12.13,280.24；P<0.01). Conclusion The suitable duration for creating a model of acute SMA ischemia reperfusion injury in small bowel is ischemia for 30 minutes and reperfusion for 2 hours.［KEY WORDS］ intestines; ischemia; reperfusion injury; model, animal缺血再灌注损伤(IRI)是指组织缺血一段时间，当血流重新恢复后，细胞功能代谢障碍及结构破坏反而较缺血时进一步加重，器官功能进一步恶化的临床综合征[1]。
探究丹参对家兔肠系膜上动脉缺血再灌注肾损伤的影响摘要：目的：初步探究丹参处理对家兔肠系膜上动脉缺血再灌注所引起的肾损伤的作用。
方法：选用健康家兔24只，随机分为3组：肠系膜上动脉缺血前注射生理盐水的对照组（Ａ组）、缺血前注射丹参注射液的实验组（B组）、再灌注前注射丹参注射液的实验组（C组）。
常规动物实验操作找到肠系膜上动脉，夹闭家兔肠系膜上动脉30min造成缺血，再灌注30min。
观察尿量，收集不同时期尿肌酐、血肌酐、丙二醛（MDA）并测定其含量。
结果：家兔肠系膜上动脉缺血再灌注后尿量减少，A组减少最显著，其次是B组，C组减少较轻。
缺血再灌注后血肌酐、血清中丙二醛浓度增加，尿肌酐浓度下降，其中A组变化最明显，其次是Ｂ组，C组变化不明显。
结论：丹参能减轻家兔肠系膜上动脉缺血再灌注所引起的肾损伤，对肾损伤有保护作用。
关键词：肠系膜上动脉缺血再灌注丹参肾损伤肠缺血再灌注（ＩＩＲ）损伤是休克、严重创伤及复苏过程中常见的病理生理过程，普遍存在肠根阻、肠扭转、大手术、烧伤等。
研究表明，小肠缺血再灌注后除对小肠组织本身造成损害外，还会通过全是炎症反应对其他器官造成损伤。
目前认为，缺血再灌注损伤产生的主要机制与活性氧的损伤作用、钙超载的作用、白细胞损伤作用、补体级联的损伤作用有关。
研究表明，丹参具有扩张外周血管、抗血栓形成、改善微循环等作用。
本实验采用家兔肠系膜上动脉缺血再灌注模型造成肠缺血，探讨丹参对家兔肠系膜上动脉缺血再灌注所致的肾损伤的保护作用和影响及其可能的机制。
1.材料与方法1.1材料：1.1.1实验动物：健康家兔２４只，体重２—３㎏，清洁级，由南方医科大学实验动物中心提供。
1.1.2主要仪器：医用计算机记录系统（PcLab）及计算机、家兔手术台、哺乳动物手术器械一套、动脉插管、静脉插管、输尿管插管、压力换能器、离心机、分光光度计，恒温水浴箱、带刻度的试管及试管夹、试管和试管架、EP管、微量加样枪带枪头、电子天平、黑色丝线、纱布等。
肾脏缺血后处理对兔急性肠道缺血再灌注损伤的保护作用目的探讨肾脏缺血后处理对兔急性肠道缺血再灌注损伤的保护作用及其可能的机制。
方法健康新西兰大白兔30只,随机分为3组(每组10只):对照组(Con)、肠道缺血后处理(IIP)、肾脏缺血后处理(RIP)。
再灌注结束后测定血浆中丙二醛(MDA)含量;实验结束后光镜下观察肠道组织形态学变化以及测定肠道组织髓过氧化物酶(MPO)活性。
结果IIP和RIP组再灌注2 h后MDA的含量明显的低于Con组(P＜0.01); SIP和RIP组再灌注2 h后组织MPO的活性明显的低于Con组(P＜0.01); SIP和RIP组再灌注2 h后光镜下观察肠道组织形态学变化证实肠道损伤程度的明显的轻于Con组(P＜0.01)。
结论肠道缺血再灌注前肾脏短暂缺血再灌注对肠道缺血再灌注损伤具有显著保护作用。
这种远离器官缺血后处理对肠道保护作用可能与减轻活性氧的损伤及抗氧化作用加强有关。
【Abstract】Objective In this study, we tested the hypothesis that remote postconditioning induced by a single 5-min episode of renal artery occlusion and reperfusion applied immediately before the onset ofsuperior mesenteric artery reperfusion protects the intestinal from reperfusion injury. Methods 30 healthy New Zealand white rabbits were randomly divided into three groups(n=10 in each group): control, intestinal ischemic postconditioning (IIP), and renal ischemic postconditioning (RIP). Tissue myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels were determined and the morphology of some intestinal tissues was observed by microscopy at the end of the experiment. Results MDA levels and MPO activity was significantly reduced in IIP and RIP as compared to control (P＜0.01),also was confirmed by the score of intestinal mucosal injury. Conclusion Renal postconditioning provides potent intestinal mucosal injury. The potential mechanism of remote postconditioning might be associated decreasing the injury caused by oxygen free radicals and strengthening the action of antioxidation.【Key words】Remote postconditioning;Ischemia - reperfusion injury;Acute intestinal ischemia越来越多的肠系膜上动脉栓塞和血栓形成早期发现早期手术治疗肠缺血再灌注损伤越来越受到临床医生的重视。
