分子生物学克隆表达常用载体序列

pcDNA3.1载体序列：5428bp

GACGGA TCGGGAGA TCTCCCGA TCCCCTA TGGTGCACTCTCAGTACAA TCTGCTCTGA TGCCGCA TAGTTAAGCCAGTA TCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAG CAAAA TTTAAGCTACAACAAGGCAAGGCTTGACCGACAA TTGCA TGAAGAA TCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGA TGTACGGGCCAGA TA TACGCGTTGACA TTGA TTA TTG ACTAGTTA TTAA TAGTAA TCAA TTACGGGGTCA TTAGTTCA TAGCCCA TA TA TGGAGTTCCGCGTTACA TAACTTACGGTAAA TGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCA TTGACG TCAA TAA TGACGTA TGTTCCCA TAGTAACGCCAA TAGGGACTTTCCA TTGACGTCAA TGGGTGGAGTA TTTACGGTAAACTGCCCACTTGGCAGTACA TCAAGTGTA TCA TA TGCCAAGTACGCC CCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCTGGCA TTA TGCCCAGTACA TGACCTTA TGGGACTTTCCTACTTGGCAGTACA TCTACGTA TTAGTCA TCGCTA TTACCA TGGTGA TGCGGTT TTGGCAGTACA TCAA TGGGCGTGGA TAGCGGTTTGACTCACGGGGA TTTCCAAGTCTCCACCCCA TTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAA TCAACGGGACTTTCCAAAA TGTCG TAACAACTCCGCCCCA TTGACGCAAA TGGGCGGTAGGCGTGTACGGTGGGAGGTCTA TA TAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTA TCGAAA TTAA TACGACT CACTA TAGGGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGA TCCACTAGTCCAGTGTGGTGGAA TTCTGCAGA TA TCCAGCACAGTGGCGGCCGCTCGAGTCTAG AGGGCCCGTTTAAACCCGCTGA TCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCA TCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAA T AAAA TGAGGAAA TTGCA TCGCA TTGTCTGAGTAGGTGTCA TTCTA TTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGA TTGGGAAGACAA TAGCAGGCA TGCTGGGGA TGCGGTG GGCTCTA TGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTA TCCCCACGCGCCCTGTAGCGGCGCA TTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACT TGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAA TCGGGGGCTCCCTTTAGGGTTCCGA TTTAGTGCTTTACGGC ACCTCGACCCCAAAAAACTTGA TTAGGGTGA TGGTTCACGTAGTGGGCCA TCGCCCTGA TAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAA TAGTGGACTCTTGTTCCAAAC TGGAACAACACTCAACCCTA TCTCGGTCTA TTCTTTTGA TTTA TAAGGGA TTTTGCCGA TTTCGGCCTA TTGGTTAAAAAA TGAGCTGA TTTAACAAAAA TTTAACGCGAA TTAA TTCTGTGGAAT GTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTA TGCAAAGCA TGCA TCTCAA TTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAA GTA TGCAAAGCA TGCA TCTCAA TTAGTCAGCAACCA TAGTCCCGCCCCTAACTCCGCCCA TCCCGCCCCTAACTCCGCCCAGTTCCGCCCA TTCTCCGCCCCA TGGCTGACTAA TTTTTTTTA TTT A TGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTA TTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTA TA TCCA TTTTCGGA TCTGA TCA AGAGACAGGA TGAGGA TCGTTTCGCA TGA TTGAACAAGA TGGA TTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTA TTCGGCTA TGACTGGGCACAACAGACAA TCGGCTGCTCTG A TGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAA TGAACTGCAGGACGAGGCAGCGCGGCTA TCGTGGCTGGCCACGACG GGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTA TTGGGCGAAGTGCCGGGGCAGGA TCTCCTGTCA TCTCACCTTGCTCCTGCCGAGAAAGTA TC CA TCA TGGCTGA TGCAA TGCGGCGGCTGCA TACGCTTGA TCCGGCTACCTGCCCA TTCGACCACCAAGCGAAACA TCGCA TCGAGCGAGCACGTACTCGGA TGGAAGCCGGTCTTGTCGA TCA GGA TGA TCTGGACGAAGAGCA TCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCA TGCCCGACGGCGAGGA TCTCGTCGTGACCCA TGGCGA TGCCTGCTTGCCGAA TA TCA TGGTGGAAAA TGGCCGCTTTTCTGGA TTCA TCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTA TCAGGACA TAGCGTTGGCTACCCGTGA TA TTGCTGAAGAGCTTGGCGGCGAA TGGG CTGACCGCTTCCTCGTGCTTTACGGTA TCGCCGCTCCCGA TTCGCAGCGCA TCGCCTTCTA TCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAA TGACCGACCAAGCGACG CCCAACCTGCCA TCACGAGA TTTCGA TTCCACCGCCGCCTTCTA TGAAAGGTTGGGCTTCGGAA TCGTTTTCCGGGACGCCGGCTGGA TGA TCCTCCAGCGCGGGGA TCTCA TGCTGGAGTTCT TCGCCCACCCCAACTTGTTTA TTGCAGCTTA TAA TGGTTACAAA TAAAGCAA TAGCA TCACAAA TTTCACAAA TAAAGCA TTTTTTTCACTGCA TTCTAGTTGTGGTTTGTCCAAACTCA TCAA TG TA TCTTA TCA TGTCTGTA TACCGTCGACCTCTAGCTAGAGCTTGGCGTAA TCA TGGTCA TAGCTGTTTCCTGTGTGAAA TTGTTA TCCGCTCACAA TTCCACACAACA TACGAGCCGGAAGCA TAA AGTGTAAAGCCTGGGGTGCCTAA TGAGTGAGCTAACTCACA TTAA TTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCA TTAA TGAA TCGGCCAACGCGCGG GGAGAGGCGGTTTGCGTA TTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTA TCAGCTCACTCAAAGGCGGTAA TACGGTTA TCCACAG AA TCAGGGGA TAACGCAGGAAAGAACA TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCA TAGGCTCCGCCCCCCTGACGAGCA TCACAA AAA TCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTA TAAAGA TACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGA TACCTGTC CGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCA TAGCTCACGCTGTAGGTA TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG CTGCGCCTTA TCCGGTAACTA TCGTCTTGAGTCCAACCCGGTAAGACACGACTTA TCGCCACTGGCAGCAGCCACTGGTAACAGGA TTAGCAGAGCGAGGTA TGTAGGCGGTGCTACAGAGTT CTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTA TTTGGTA TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGA TCCGGCAAACAAACCACCGC TGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGA TTACGCGCAGAAAAAAAGGA TCTCAAGAAGA TCCTTTGA TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA T TTTGGTCA TGAGA TTA TCAAAAAGGATCTTCACCTAGA TCCTTTTAAA TTAAAAA TGAAGTTTTAAA TCAA TCTAAAGTA TA TA TGAGTAAACTTGGTCTGACAGTTACCAA TGCTTAA TCAGTGA GGCACCTA TCTCAGCGA TCTGTCTA TTTCGTTCA TCCA TAGTTGCCTGACTCCCCGTCGTGTAGA TAACTACGA TACGGGAGGGCTTACCA TCTGGCCCCAGTGCTGCAA TGA TACCGCGAGACC CACGCTCACCGGCTCCAGA TTTA TCAGCAA TAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTA TCCGCCTCCA TCCAGTCTA TTAA TTGTTGCCGGGAAGCTAGAG TAAGTAGTTCGCCAGTTAA TAGTTTGCGCAACGTTGTTGCCA TTGCTACAGGCA TCGTGGTGTCACGCTCGTCGTTTGGTA TGGCTTCA TTCAGCTCCGGTTCCCAACGA TCAAGGCGAGTTACA TGA TCCCCCA TGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGA TCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTA TCACTCA TGGTTA TGGCAGCACTGCA TAA TTCTCTTACTGTCA T GCCA TCCGTAAGA TGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCA TTCTGAGAA TAGTGTA TGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAA TACGGGA TAA TACCGCGCCACA TAGC AGAACTTTAAAAGTGCTCA TCA TTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGA TCTTACCGCTGTTGAGA TCCAGTTCGA TGTAACCCACTCGTGCACCCAACTGA TCTTCAGCA TCTT TTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAA TGCCGCAAAAAAGGGAA TAAGGGCGACACGGAAA TGTTGAA TACTCA TACTCTTCCTTTTTCAA TA TTA TTGAAGCA TTTA TCAGGGTTA TTGTCTCA TGAGCGGA TACA TA TTTGAA TGTA TTTAGAAAAATAAACAAA TAGGGGTTCCGCGCACA TTTCCCCGAAAAGTGCCACCTGACGTC