The single-strand binding protein (SSB) attaches to singlestranded DNA, thereby preventing the DNA from forming a duplex
防止单链复性
维持单链刚性状态
避免单链降解
The SSB binds as a monomer, but typically in a cooperative manner in which the binding of one protein molecule makes it much easier for another to bind
• mtDNA的复制在H链的复制起点OH开始合成; • 当置换链通过L链的复制起点OL时，开始合成新的H链; • 新的L链合成完成，子代双链被释放。
Key concepts: Mitochondria use different origin sequences
③Rolling circles（φX174
4. Restart of stalled replication forks :
Initiation of φX174 replication requires the primosome complex to displace SSB from the origin. The primosome consists of six proteins: PriA, PriB, PriC, DnaT, DnaB, and DnaC. A primosome assembles at a unique site, called the
五、旋转酶(gyrase )：
a Type II topoisomerase, acts to overcome the torsional stress imposed upon unwinding by introducing negative supercoils at the expense of ATP hydrolysis.
