β—七叶皂甙钠对严重烫伤早期大鼠胶质原纤维酸性蛋白及突触变化的影响

应用β-七叶皂甙钠治疗大鼠脊髓损伤的实验研究王金光;郑启新;郭晓东;王运涛【期刊名称】《中国康复医学杂志》【年(卷),期】2005(020)001【摘要】目的:观察应用β-七叶皂甙钠治疗大鼠脊髓损伤后功能恢复、损伤段神经细胞凋亡表达情况.方法:将22只SD大鼠根据损伤及治疗情况分为3组:空白组、损伤组、治疗组,观察药物治疗前后大鼠的神经功能行为的变化.损伤后12天处死动物,损伤段脊髓标本做组织学切片,HE染色;做免疫组化检测凋亡因子(包括bcl-2,bax,faxs);TUNEL标记凋亡细胞.结果:治疗组在用药后,神经功能有所提高,且与损伤组比较,效果显著(P＜0.05).凋亡指标检测:fas、bax、TUNEL显示,损伤组的凋亡阳性细胞明显多于治疗组;bcl-2显示,治疗组的阳性表达明显多于损伤组(P＜0.05).结论:β-七叶皂甙钠能抑制脊髓神经细胞凋亡,对损伤脊髓的神经功能具有保护作用.【总页数】3页(P15-17)【作者】王金光;郑启新;郭晓东;王运涛【作者单位】华中科技大学同济医学院附属协和医院骨科,武汉市,430022;华中科技大学同济医学院附属协和医院骨科,武汉市,430022;华中科技大学同济医学院附属协和医院骨科,武汉市,430022;华中科技大学同济医学院附属协和医院骨科,武汉市,430022【正文语种】中文【中图分类】R493;R651.2【相关文献】1.应用MRI观察β—七叶皂甙钠对大鼠脑出血后脑水肿的治疗应用 [J], 陈旭;郑惠民2.β-七叶皂甙钠对脊髓损伤大鼠保护作用的实验研究 [J], 丁勇;吴玉杰;傅智轶;金文杰;胡小鹏;刘兴振3.脱落乳牙牙髓干细胞治疗大鼠脊髓损伤的实验研究 [J], 刘露;翟启明;张青;刘安琪;刘文佳;金钫4.外置磁场下静脉注射经SPIONs标记的Ngb转染ADSCs靶向治疗大鼠脊髓损伤的实验研究 [J], 胡金金;齐新文;李松军;王德伟;谭伟源5.脊椎治疗方治疗大鼠脊髓损伤的实验研究 [J], 王慧敏;谭明生;杨洪艳;移平;陈博来;邓晋丰因版权原因，仅展示原文概要，查看原文内容请购买。

β-七叶皂甙钠对大鼠脊髓损伤后肢体运动功能的影响白玉树;傅强;朱晓东;倪春鸿;沈洪兴;贺石生;李明;侯铁胜【期刊名称】《中国组织工程研究》【年(卷),期】2005(009)033【摘要】目的:观察β-七叶皂甙钠治疗大鼠急性脊髓损伤后肢体运动功能的变化,探讨β-七叶皂甙钠对大鼠急性脊髓损伤的保护和促进修复作用.方法:实验于2003-12/2004-03在解放军第二军医大学长海医院骨科实验室完成.45只SD大鼠随机分为正常对照组11只,模型对照组12只,10 mg/(kg·d)β-七叶皂甙钠治疗组11只,20 mg/(kg·d)β-七叶皂甙钠治疗组11只.按Allen改良法制备脊髓损伤动物模型,10 mg/(kg·d)和20 mg/(kg·d)β-七叶皂甙钠治疗组分别在术后腹腔注射β-七叶皂甙钠注射液10 mg/(kg·d)和20 mg/(kg·d).模型对照组和正常对照组在术后注射等量生理盐水,共持续10 d.用斜板试验法和BBB评分法观察大鼠脊髓损伤后运动功能的变化.结果:1只10 mg/kg β-七叶皂甙钠治疗组大鼠在术后第2天和1只正常对照组大鼠在术后第1天由于饲养不当死亡,其余43只大鼠均进入结果分析.①各组大鼠斜板试验临界角度:在术后第7,14,21天时10 mg/(kg·d)和20mg/(kg·d)β-七叶皂甙钠治疗组明显高于模型对照组[第7天:(37.88±1.76)°,(38.94±2.15)°,(35.48±1.23)°;第14天:(44.39±1.87)°,(45.96±1.81)°,(39.48±1.23)°;第21天:(44.75±2.11)°,(46.66±1.46)°,(41.42±0.98)°,t=2.27～4.67,P＜0.05～0.01].20 mg/(kg·d)β-七叶皂甙钠治疗组与10 mg/(kg·d)β-七叶皂甙钠治疗组基本一致.②各组大鼠BBB评分结果:在术后第7,14,21天时10 mg/(kg·d)和20 mg/(kg·d)β-七叶皂甙钠治疗组明显高于模型对照组[第7天:(7.20±0.98),(7.51±0.85),(4.46±0.90)分;第14天:(10.43±0.90),(10.98±0.59),(6.54±0.73)分;第21天:(10.78±0.96),(11.67±0.49),(7.90±0.54)分,t=2.54～8.6,P＜0.05～0.01].20 mg/(kg·d)β-七叶皂甙钠治疗组与10 mg/(kg·d)β-七叶皂甙钠治疗组基本一致.结论:β-七叶皂甙钠可以增加大鼠实验性急性脊髓损伤后运动功能的恢复速度及最终恢复程度.【总页数】2页(P90-91)【作者】白玉树;傅强;朱晓东;倪春鸿;沈洪兴;贺石生;李明;侯铁胜【作者单位】解放军第二军医大学长海医院骨科,上海市,200433;解放军第二军医大学长海医院骨科,上海市,200433;解放军第二军医大学长海医院骨科,上海市,200433;解放军第二军医大学长海医院骨科,上海市,200433;解放军第二军医大学长海医院骨科,上海市,200433;解放军第二军医大学长海医院骨科,上海市,200433;解放军第二军医大学长海医院骨科,上海市,200433;解放军第二军医大学长海医院骨科,上海市,200433【正文语种】中文【中图分类】R744【相关文献】1.夹脊电针联合甲强龙对急性脊髓损伤大鼠肢体运动功能及尼氏小体影响的实验研究 [J], 李晓宁;吴磊;梅继林;李诺;单筱淳;梁雪松2.重组人红细胞生成素与β-七叶皂甙钠对大鼠脊髓损伤后功能恢复和神经细胞继发性损伤的影响 [J], 赵亮;曹阳;徐莉3.β-七叶皂甙钠对脊髓损伤大鼠脊髓组织水通道蛋白4与水通道蛋白9表达的影响 [J], 陈勇;赤仁杰4.β-七叶皂甙钠对大鼠脊髓损伤段钙、水含量的影响及其保护作用 [J], 郭开今;陈向阳;张志明;唐天驷;杨惠林;袁峰5.微囊化异种嗅球组织移植联合β-七叶皂甙钠对脊髓损伤大鼠血脊髓屏障功能和血管源性脊髓水肿的影响 [J], 王航辉;张博;朱文侠;李小记;韩振奎;王璐;赵琳;刘伯锋因版权原因，仅展示原文概要，查看原文内容请购买。

CHIN ESE J OU RNAL OF ANA TOM Y Vol.32No.12009解剖学杂志　2009年第32卷第1期大鼠严重烧伤后脑内胶质原纤维酸性蛋白表达变化及意义ΞΞ江西省卫生厅课题(20062028)作者E 2mail :lzx6698@ 收稿日期:200826211;修回日期:2008209228刘曾旭　刘德明　王航辉　王向东　万　斌　周聪发　胡维晟(南昌大学基础医学院解剖学教研室,南昌　330006)摘要　目的:探讨严重烫伤早期脑内胶质原纤维酸性蛋白(GFA P )表达变化与血脑屏障通透性变化的关系。

方法:SD 大鼠随机分为正常对照组、假烫组、烫伤组,其中烫伤组又分为烫伤后3、6、12、24、48h 等5组,建立30%Ⅲ度烫伤模型。

应用免疫组织化学方法检测脑内GFA P 表达变化,用化学法测量脑组织内伊文蓝的含量及脑百分含水量,并分析它们之间的关系。

结果:烫伤3～6h GFA P 阳性细胞数目明显增多,染色增强,平均积分光密度较假烫组明显升高,烫伤后12～24h 阳性细胞数达高峰,48h 脑组织内阳性细胞数与对照组比较仍明显增多。

严重烫伤后脑百分含水量及脑内伊文蓝含量增高,以6～12h 最为显著。

结论:严重烫伤后GFA P 表达上升可能与血脑屏障通透性增高有关。

关键词　烫伤;血脑屏障;胶质原纤维酸性蛋白;大鼠Change of glial f ibrillary acidic protein expression in rat brainfollowing severe burn and its signif icance ΞLiu Zengxu ,Liu Deming ,Wang Hanghui ,Wang Xiangdong ,Wan Bin ,Zhou Chongfa ,Hu Weisheng(Department of A natom y ,N anchang Universit y ,N anchang 330006,China )Abstract Obj ective :To investigate relations between the changes of glial fibrillary acidic protein (GFAP )and blood 2brain barrier (BBB )permeability following severe burn in the rat brain.Met hods :In this study ,healthy Sprague 2Dawley rats were divided into three group s as follows :normal control ,sham control ,scald group which were subdivided into five post 2scald group s :3,6,12,24and 48h.Changes in BBB permeability were determined via Evan blue content in the brain with a chemical method.Brain edema was detected by calculating water content.Furthermore ,to explore the molecular mechanism of BBB disruption in the brain after severe scalds ,GFA P expression in the brains post burn were detected by immunohisto 2chemistry analysis.Results :Brain water content and Evan blue dye were significantly increased after scald.The expression of brain GFA P and integral optic density (IOD )began to increase at 326h ,reached significant levels between 12and 24h af 2ter thermal injury compared to the sham controls ,and remained at 48h (P <0101).Conclusion :The increase in GFAP ex 2pression may associate with the increased BBB permeability following thermal injury.K ey w ords scald ;blood 2brain barrier ;glial fibrillary acidic protein ;rat 星形胶质细胞(ast rocytes ,AS )终足紧密包绕血管壁,可能在诱导和维持血脑屏障中起关键作用[1],为血脑屏障(blood 2brain barrier ,BBB )维持内环境稳定的第三道屏障,对于维持BBB 的功能具有重要意义。

β-七叶皂甙钠对大鼠脑缺血-再灌注损伤的保护作用杨志勇;万海涛;程贤亮【期刊名称】《中国实用神经疾病杂志》【年(卷),期】2015(000)007【摘要】Objective To investigate the protective effects ofβ‐seven sodium saponin on cerebral ischemia‐reperfusion inju‐ry in rat.Methods 74 healthy Sprague‐Dawley rats were selected and divided into sham operation group ,ischemia reperfusion (IR) group ,the treatment group. The preparation of focal cerebral ischemia reperfusion model was made. After 48 h reperfu‐sion ,the volume of cerebral infarction ,cerebraledema ,detection of neurological function score ,NISSL staining ,TUNEL stai‐ning and caspase‐3 activity were tested.Results After treatment ,the volume of cerebral infarction was significantly decreased (17 ± 8)%. In I/R group ,the brain water content was significantly increased compared to the normal control and sham opera‐tion group ,while the cerebral water content decreased significantly treated with seven sodium saponin. Compared with the sham operation group ,the TUNEL positive cells in the cortex and hippocampus of I/R gr oup increased significantly ,whileβ‐seven sodium saponin treatment could significantly reduce the number of TUNEL positive cells. Caspase‐3 in the cortex and hippocampus activity of treatment group was significantly lower than that in I/R group. Conclusion β‐Seven sodium saponin treatment can effectively reduce the volume ofcerebral infarction ,brain edema ,manifested as nerve function is improved , w hich may be related to treatment can increase the survival number of neurons and inhibit neuronal apoptosis (apoptosis related enzyme activity).%目的：探讨β‐七叶皂甙钠对大鼠脑缺血‐再灌注损伤的保护效果。

β-七叶皂甙钠对大鼠脑缺血再灌注损伤后血脑屏障功能和胶质原纤维酸性蛋白表达的影响李芸【期刊名称】《四川医学》【年(卷),期】2010(31)10【摘要】目的观察β-七叶皂甙钠对大鼠脑缺血再灌注损伤后血脑屏障功能和GFAP表达的影响.探讨其对脑缺血再灌注损伤的保护作用及其机制.方法 60只SD 大鼠随机分为假手术组对照组β-七叶皂甙钠治疗组各20只.线栓法制作大鼠脑缺血再灌注模型,再灌注48h后干湿重法测定脑含水量,用伊文思蓝测定血脑屏障通透性,免疫组织化学技术检测GFAP的表达,同时电镜观察血脑屏障的变化.结果脑缺血再灌注48h,对照组缺血侧脑含水量明显升高,电镜观察发现血脑屏障破坏严重,GFAP的表达也较假手术组显著增加;而应用β-七叶皂甙钠处理的大鼠其缺血脑含水量明显减少,同时电镜观察到血脑屏障的破坏减轻,而GFAP表达水平也明显低于对照组(P<0.05).结论β-七叶皂甙钠对大鼠脑缺血再灌注损伤后的血脑屏障具有保护作用,其机制可能与抑制GFAP表达,减轻脑水肿有关.【总页数】4页(P1429-1432)【作者】李芸【作者单位】延安大学医学院解剖学教研室,陕西,延安,716000【正文语种】中文【中图分类】R-332【相关文献】1.七叶皂甙钠对局灶性脑缺血再灌注损伤大鼠 Bcl-2 和Caspase-3表达及细胞凋亡的影响 [J], 范学军;郭科;肖波;资晓宏;宋治2.L-硝基-精氨酸甲酯对大鼠脑缺血再灌注损伤后血脑屏障功能和基质金属蛋白酶-9表达的影响 [J], 李小记;王航辉;米志宽;惠雪枫3.水通道蛋白4基因干预对大鼠脑缺血再灌注损伤后血脑屏障形态及功能的影响[J], 石向群;杨金升;张志琳;王运良;包仕尧4.运动预处理对大鼠脑缺血再灌注损伤后血脑屏障通透性的影响 [J], 梁碧莹;朱路文;唐强;叶涛;李宏玉;李保龙;阮野5.大鼠脑缺血再灌注损伤后HMG-COA还原酶抑制剂下调Caveolin-1表达对血脑屏障的影响 [J], 王也;冯新红;毕国荣;武剑因版权原因，仅展示原文概要，查看原文内容请购买。

β-七叶皂甙钠对大鼠缺血性脑水肿及水通道蛋白4表达的影响鹿寒冰;李晓宾;董瑞国;李传玲【期刊名称】《中国脑血管病杂志》【年(卷),期】2009(6)6【摘要】目的研究β - 七叶皂甙钠对大鼠脑梗死后脑水肿及水通道蛋白4(AQP4)表达的影响. 方法取SD雄性大鼠150只,体质量240～300 g.随机分为β - 七叶皂甙钠治疗组60只,对照组60只,假手术组30只.各组大鼠又按缺血时间不同(6 h和1、3、5、7 d)分为5个亚组,其中治疗组和对照组每亚组各12只,假手术组每亚组6只.用线栓法制作大鼠大脑中动脉缺血模型,向治疗组大鼠腹腔注射β - 七叶皂甙钠(5 mg·kg-1·d-1),对照组及假手术组在相应时间点注射等量等渗盐水.于造模后6 h和1、3、5、7 d断头取脑.采用干湿重法观察脑水肿的动态变化,通过HE染色观察脑梗死区的病理学变化,应用免疫组化方法检测脑组织AQP4的表达. 结果① 脑梗死后6 h,病变侧大脑半球脑含水量开始增多,至3 d时达高峰,以后逐渐下降,7 d 时仍高于假手术组.治疗组及对照组脑含水量组内不同时间点比较差异均有统计学意义(F=30.704,F=36.703,均P<0.01);各时间点治疗组及对照组梗死侧大脑半球脑含水量均明显高于假手术组(均P<0.01),治疗组均低于对照组(P <0.05～0.01).②脑梗死后6 h,梗死侧AQP4表达水平即增高,3 d时达高峰,以后逐渐下降,7 d时仍高于假手术组;治疗组和对照组各组内各时间点比较差异均有统计学意义(F=20.878,F=28.489,均P<0.01).治疗组及对照组各时间点AQP4的表达均高于假手术组(均P<0.01),治疗组AQP4的表达均明显低于对照组(P<0.05～0.01).AQP4表达水平与脑含水量呈正相关(r=0.972, P<0.001). 结论β - 七叶皂甙钠能明显减轻脑梗死后脑水肿及降低AQP4的表达,β - 七叶皂甙钠可能通过抑制AQP4的表达而减轻脑水肿.【总页数】6页(P298-303)【作者】鹿寒冰;李晓宾;董瑞国;李传玲【作者单位】221009,徐州市中心医院神经内科;221009,徐州市中心医院神经内科;徐州医学院附属医院神经内科;221009,徐州市中心医院神经内科【正文语种】中文【中图分类】R743;R363【相关文献】1.水通道结合膜蛋白-4在大鼠缺血性脑水肿中的表达变化 [J], 彭雪华;孙善全2.β-七叶皂甙钠对脊髓损伤大鼠脊髓组织水通道蛋白4与水通道蛋白9表达的影响 [J], 陈勇;赤仁杰3.亚低温对大鼠缺血性脑水肿及水通道蛋白AQP4表达的影响 [J], 阎蕾;董瑞国;曾因明;耿德勤4.七氟醚与大鼠缺血性脑水肿脑组织内水通道蛋白4表达的影响 [J], 陈文斌;林思永;冯作炫5.β-七叶皂甙钠对脑缺血小鼠脑组织白介素-1β、水通道蛋白-4表达及脑梗死体积和脑水肿的影响 [J], 沙杜鹃;李启明;李瑾;朱震寒;张均因版权原因，仅展示原文概要，查看原文内容请购买。

β-七叶皂甙钠抑制损伤脊髓细胞胶质纤维酸性蛋白的表达丁勇;吴玉杰;傅智轶;金文杰;胡小鹏;刘兴振【期刊名称】《中国组织工程研究》【年(卷),期】2014(000)015【摘要】BACKGROUND:The methylprednisolone pulse therapy in early period of spinal cord injury can attenuate the pathological degree of spinal cord injury, however no breakthrough was found within recent 20 years. <br> OBJECTIVE:To observe the protection effects of sodium aescinate on the nerve cellapoptosis and expression of glial fibrial ary acidic protein (GFAP) in the early spinal cord injured rats. <br> METHODS:Spinal cord injury models were established with the modified Al en’s method in 180 Sprague-Dawley rats, and were randomly divided into three groups, with 60 rats in each group. Immediately after injury, the rats in three groups were intraperitoneal y injected with sodium aescinate (5 mg/kg), methylprednisolone (100 mg/kg) and equal saline, respectively, once per day. At 8 hours, 24 hours, 96 hours and 7 days, 14 days after injury, rats were sacrificed and the injured segments were resected for hematoxylin-eosin staining and immunohistochemical staining, the nerve cellapoptosis and GFAP expression were detected. <br> RESULTS AND CONCLUSION:The apoptotic nerve cells were seen at 8 hours after injury and the number of apoptotic cells reached the peak at 7 days, the edema was attenuated at 14 days without less nerve cellapoptosis in al groups,significantly fewer apoptotic nerve cells can be seen in sodium aescinate and methylprednisolone groups compared with the control group (P<0.05) at each time. The expression of GFAP&nbsp;was increased in the time dependant manner in al groups, the increase was slow in methylprednisolone group but sharp in sodium aescinate group and control group within 96 hours. There was no difference between control group and sodium aescinate group within 24 hours (P>0.05), which was lower than methylprednisolone group (P<0.05);after 96 hours, methylprednisolone group and sodium aescinate group were both significantly lower than control group (P<0.05). Furthermore, the decreasing expression was observed in al groups after 7 days. Sodium ascinate has obvious protection effects on nerve cells in spinal cord injured rats and promotes neurological function through decreasing GFAP expression after injury. The efficacy of sodium ascinate is equal to that of methylprednisolone within 2 hours.%背景：研究证实脊髓损伤后早期应用甲基强的松龙冲击治疗可以减轻脊髓损伤的病理程度，但是近20年未再有突破性进展。

三七总皂苷对烫伤大鼠心肌细胞及红细胞膜ATP酶活性的影响张海港;李晓辉【期刊名称】《中国急救医学》【年(卷),期】2001(021)010【摘要】目的研究三七总皂苷(PNS)对烫伤早期心肌细胞膜(Ca2+-Mg2+)-ATP 酶和(Na+-K+)-ATP酶活性的影响.方法采用30%体表面积Ⅲ度烫伤大鼠模型,用生化反应法分别测定心肌细胞膜与红细胞膜上(Ca2+-Mg2+)-ATP酶和(Na+-K+)-ATP酶活性变化.结果大鼠烫伤后心率加快,心电图T波低平,心肌细胞与红细胞膜(Ca2+-Mg2+)-ATP酶和(Na+-K+)-ATP酶活性均显著下降(P＜0.01),(Ca2+-Mg2+)-ATP酶活性在心肌细胞与红细胞之间呈显著正相关(r=0.951,P＜0.01).100 mg/kg的PNS可使心率及T波恢复、ATP酶活性明显增加(P＜0.01或0.05).结论 PNS显著增加烫伤大鼠心肌细胞膜(Ca2+-Mg2+)-ATP酶和(Na+-K+)-ATP酶活性是其改善心功能的重要机制之一.【总页数】3页(P574-576)【作者】张海港;李晓辉【作者单位】第三军医大学药理教研室;第三军医大学药理教研室【正文语种】中文【中图分类】R966;R644【相关文献】1.不同负荷运动训练对大鼠红细胞膜的影响--Na+-K+ATP酶、Ca2+-ATP酶活性及唾液酸(SA)含量的变化 [J], 赵歌;李现武2.间歇性低氧及运动对大鼠红细胞膜Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性的影响 [J], 王绎;徐鹏霄3.三七总皂苷对烫伤大鼠红细胞及心肌细胞膜（Ca2＋—Mg2＋）—ATPase和（Na＋—K＋）—ATPase活性影响 [J], 张海港;李晓辉4.不同负荷运动训练对大鼠红细胞膜的影响——Na^+-k^+ATP酸及Ca^(2+)-ATP酶活性的变化 [J], 郑兵;赵歌;王震5.间歇性低氧及运动对大鼠红细胞膜Na^+-K^+-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶活性的影响 [J], 王绎;张斌;李邦翅;朱建文;舒心;李海英;赵娟;佟长青因版权原因，仅展示原文概要，查看原文内容请购买。