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Agilent 7100 Capillary Electrophoresis SystemHighest sensitivity. Highest productivity. Minimal footprint.Features •Most sensitive CE system on the market•Replenishment system allows high throughput and unattended operation •Easy maintenance and automated instrument diagnostics •Plug & play connectivity to all Agilent MS systems •Easy method setup data analysis •Regulatory compliance tools•Comprehensive offering of accessories and consumables •Partner solutions for LIF and electrochemical detectionBenefits •Best in class analytical performance•Broadest selection of detectors in the industry •Legendary Agilent reliability•Reverse-compatible with the Agilent HPCE •Increased uptime•Flexible separation modes including capillary electrochromatography•CE-MS support from the leading vendor with the longest CE-MS experience •Broad application coverage•Complete solution for charged sample analysisCapillary Electrophoresis (CE) offers fast separations with exceptional efficiency and resolution for analytical challenges that often can only be met with difficulty by LC. Used in standalone mode, as the separations component of a CE/MS, or as a complementary,orthogonal technology to LC, the Agilent 7100 CE system brings un-precedented HPLC-like sensitivity to a wide range of analytical challenges.In addition, CE offers the advantage that several separation modes can be run on a single instrument. This makes CE a very versatile technique for a broad range of applications and separation challenges.Application AreasCE is used to separate, identify, quantify and fractionate compounds in:•Environmental analysis •Food safety •Forensics• Pharmaceutical discovery, development, and QA/QC •Life Sciences/chem/ce© Agilent Technologies Inc., 2014-2015 Published April 1, 2015Publication Number 5990-3962ENPressure systemProgrammable with –100 to +100 mbar on inlet Flushing with 1 bar or with high pressure 2–12 bar Vial pressurization with high pressure 2–12 bar on inlet and/or outletInjection modesSelf correcting injection system with injection from inlet or outlet Programmable range: up to 10,000 seconds Pressure: –100 to +100 mbar Electrokinetic: –30 to +30 kV Autosampler/ fraction collector 50-position carouselAll vials are randomly accessible from inlet or outlet end of capillary.Temperature control with external waterbath with vial temperature from 10–40 °C.(Non-condensing conditions, minimum waterbath temperature +1 °C).ReplenishmentSatellite station for refilling of inlet or outlet buffer vials with fresh buffer for automatic, continuous operation. Selectable buffer levelling.Vials100 µL (polypropylene or glas) with resealing snap caps 1 mL (polypropylene) with resealing snap caps 2 mL (glas) with resealing snap capsCapillary cassetteHigh-speed forced-air cooler with Peltier element Temperature range: 10 °C below ambient, up to 60 °C (min. 10 °C cassette temperature)Minimum total capillary length: 31 cm Capillary compatibility 365 µm o.d.DetectorReal time UV-Visible diode-array detector (190–600 nm)Temperature controlled Wavelength resolution: 1 nm Response time: 0.025 to 10 sLight source: prealigned deuterium or high brightness lampSignals: up to eight signals simultaneously, full spectral acquisition with Agilent ChemStation Sensitivity: 1 µM 4-hydroxy-aceto-phenon injected at50 mbar • 5 sec, 3 x 50 µm bubble cell capillary, signal/noise >6* (20 mM borate buffer pH 9.3, 25 kV)Baseline noise: <50 µAULinear dynamic range: 1x104(3 x 50 µm bubble cell capillary)** typical value **upgradeableRaw data channelsDetector signals and spectrum, voltage, current, leak current, power, cassette temperature, pressure, lamp voltage and detector temperatureDiagnostic functionsRFID tag for lamp, early maintenance feedback, supported by Agilent Lab Advisor software with integrated diagnosis test suite Safety featuresCurrent leak detection: low current limit Liquid leak sensorSafety sensors at door and cover disabling high voltage Vial sensorEnvironmentTemperature: 5–40 °C Humidity: up to 80 % rel. humidity at 31 °C (non-condensing)System control Operating system:- Windows 7 SP1 (32-bit or 64-bit, Professional or Enterprise)- Windows 8.1 (32-bit or 64-bit, Professional or Enterprise)Time programmable parameters: voltage, current, power, polarity, pressure, inlet and outlet vial, capillary temperature, pre and post-run conditioning with pressure and/or voltage,Replenishment, fraction collection**CE specific softwareMobility report, time corrected areas, pI calibration and bio polymer size calibration.Physical Specifications。
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毛细管电泳的基本原理及应用摘要：毛细管电泳法是以弹性石英毛细管为分离通道，以高压直流电场为驱动力，依据样品中各组分之间淌度和分配行为上的差异而实现分离的电泳分离分析方法。
该技术可分析的成分小至有机离子、大至生物大分子如蛋白质、核酸等。
可用于分析多种体液样本如血清或血浆、尿、脑脊液及唾液等，比HPLC 分析高效、快速、微量。
关键词：毛细管电泳原理分离模式应用1概述毛细管电泳(Caillary Electrophoresis)简称CE，是一类以毛细管为分离通道，以高压直流场为驱动力的新型液相分离分析技术。
CE的历史可以追溯到1967年瑞典Hjerten最先提出在直径为3mm的毛细管中做自由溶液的区带电泳(Capillary Zone Electro-phoresis，CZE)。
但他没有完全克服传统电泳的弊端[1]。
现在所说的毛细管电泳(CE)是由Jorgenson和Lukacs在1981年首先提出，他们使用了75mm的毛细管柱，用荧光检测器对多种组分实现了分离。
1984年Terabe将胶束引入毛细管电泳，开创了毛细管电泳的重要分支: 胶束电动毛细管色谱(MEKC)。
1987年Hjerten等把传统的等电聚焦过程转移到毛细管内进行。
同年，Cohen 发表了毛细管凝胶电泳的工作。
近年来，将液相色谱的固定相引入毛细管电泳中，又发展了电色谱，扩大了电泳的应用范围。
毛细管电泳和高效液相色谱（HPLC）一样，同是液相分离技术，因此在很大程度上HPCE与HPLC可以互为补充，但是无论从效率、速度、样品用量和成本来说，毛细管电泳都显示了一定的优势毛细管电泳（C E）除了比其它色谱分离分析方法具有效率更高、速度更快、样品和试剂耗量更少、应用面同样广泛等优点外，其仪器结构也比高效液相色谱（HPLC）简单。
C E只需高压直流电源、进样装置、毛细管和检测器。
毛细管电泳具有分析速度快、分离效率高、试验成本低、消耗少、操作简便等特点，因此广泛应用于分子生物学、医学、药学、材料学以及与化学有关的化工、环保、食品、饮料等各个领域[2]。
Agilent 7100毛细管电泳系统Our measure is your success.产品|应用|软件|服务最高的灵敏度最高的效率Agilent 7100毛细管电泳系统为您最具挑战性的分离提供更高的灵敏度毛细管电泳（CE）为带电物质，比如生物分子、小分子碱性或酸性药物和离子提供快速分离、出色的效率和分离度，而这些分离使用HPLC常常难以实现。
CE还适合于样品量很有限的分离应用，所需要的缓冲液要比液相色谱或离子色谱少得多。
使用单机模式、作为CE/MS的分离组件、或者作为液相色谱的辅助和正交技术，新的Agilent 7100 CE 系统对于广泛的分析挑战带来了无可匹敌的、与HPLC相当的灵敏度。
该系统具有业内最佳的分析性能，是业内可配置检测器类型最多的毛细管电泳系统，而且与安捷伦6000系列质谱仪完全兼容。
享有盛誉的安捷伦可靠性、熟悉的化学工作站软件和同一供货商解决方案，所有这些都使得Agilent 7100物超所值，成为您实验室下一台（或第一台）CE或CE/MS 系统的首选。
体积更小、噪音更低、更省电的Agilent 7100毛细管电泳系统具有无可匹敌的灵敏度和操作性能，而且与安捷伦上一代毛细管电泳系统100%的兼容如需了解有关Agilent 7100 CE系统的更多信息，请访问/chem/ce:cn23世界上最耐用，最易拥有的CE7100 CE 系统集中了工作效率高、可靠性强和易于使用的特点，采用耐用的内部压力系统和改进的毛细管冷却功能，可使用更大内径的毛细管。
此外，改进的缓冲液更新系统允许更长时间的无人看管操作，新的样品瓶传感器防止了运行前或运行中装载样品瓶时可能发生的冲突。
系统的模块式结构允许快速而简便地接触电极和预穿孔器、电子线路板和管线，以便进行日常维护和服务。
更快速换、自定位的毛细管卡套– 与所有市售毛细管兼容– 可在几分钟内完成安装。
安捷伦的实验室监控与诊断软件使得正常运行时间最大化，在故障发生之前就可确定。
