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User GuideMillicell® Ultra-low Attachment Plates96-well, U-bottom, clear polystyreneMC96ULA20Product DescriptionThe Millicell® Ultra-low Attachment (ULA), 96-well,U-bottom, clear plates are used to generate homogeneous spheroid cultures.Each well contains a specialized ULA ultra-hydrophilic polymer coating that enables spontaneous spheroid formation of uniform size and shape, and prevents cells from adhering. Coupled with the U-shaped round bottom, the Millicell® ULA Plates enable the production, culture, and analysis of spheroids and other 3D cell structures. The initial cell aggregate size is highly uniform and can be controlled by adjusting the initial cell seeding. The clear Millicell® ULA Plates offer great visibility of aggregate formation and growth of the 3D cultures over time.Millicell® ULA Plates promote scaffold free,self-assembly of spheroid formation. The Millicell®ULA Plates have high optical clarity making them highly suitable for brightfield imaging and confocal microscopy.Millicell® ULA Plates are gamma irradiated in packaging. Features• Millicell® ULA Plates have well numbers indicated on both top and bottom of each column.• The frosted effect on the plate enhances the ability to read the column and row indication labels.• The plate has an opening on the on the long sideof the bottom of the plate for easier grip.• The Millicell® ULA Plate lid fits the plate with rings designed to frame the top of each well to impede evaporation.• The round U-shaped bottom of the Millicell® ULA Plate provides optimum spheroid growth.• Stable, non-cytotoxic and cell non-adhesionsurface• Easy to handle, compatible with liquid roboticsystem• Round bottom wells have high optical claritymaking them suitable for bright field imagingand confocal microscopy.• Uniform single spheroid formation in each well 3D Spheroid Applications• Fluorescent Image Analysis, cell divisionin spheroids• Fluorescent Image Analysis, live dead cellassay of spheroids• Evaluations of Drug Efficacy, the responseof co-cultured spheroids to drugs• Analysis of Anticancer Drugs, targetingthe interior of spheroid• Evaluation of Drug Efficacy, quantitative evaluation of spheroid size• Evaluation of Drug Efficacy, the responseof spheroids to a drug• Cell Counting, measuring the numberof cells in spheroidsStorageStore Millicell® ULA Plates at room temperature and low humid location away from direct sunlight. Do not use if package is wet or damaged.Chemical Resistance0.1%SpecificationsPlate Type 96-wellPlate Material Clear polystyrene Lid Material Clear polystyrene Well VolumeMax 300 μL <250 μLrecommendedWell Depth10 mm Well Diameter Top/Bottom 7.0/6.1mm Plate Length 127.5 mm Plate Width 85.9 mm Plate Height 14 mm A1 Row Offset 11.45 mm A1 Column Offset14.25 mmWell Center to Well Center Spacing9 mm Flange or Skirt Height 3.5 mm Stack Height12.9 mmWell Bottom Elevation 4 mm Well Bottom Thickness 1.1 mm Distance to Bottom of Plate2.9 mmCompatible with brightfield and fluorescenceimaging systems.Directions for UsePlease read the entire protocol before proceeding.PreparationMillicell® ULA Plates with ULA coating on the well surface, received gamma irradiated in its packaging. To prevent contamination, plates should be opened inside a bio-safety cabinet.Generation of Cell Aggregates from a Single Cell SuspensionMultiple cell lines were shown to form single, centered spheroids in 96-well microplate formats. Brightfield images were taken after incubating samples for 72 hours (10x and 4x magnification).A549HeLa HepG2MCF7Cells grow and divide to formspheroids within 24-72 hoursBrightfield images of spheroidsSeeding Cells in wells1. Culture cells in 2D cell culture flask until they are between 60-80% confluence.2. Aspirate media from the culture flask.3. Add sterile PBS for 3 minutes at room temperature.4. Aspirate liquid from the culture flask.5. Add Trypsin to the culture flask and incubate at 37 °C 5% carbon dioxide cell culture incubator.6. Stop the trypsin reaction by adding equal parts complete cell culture media to the flask.7. Collect liquid suspension and spin down in the centrifuge at 300 x g for 3 minutes.8. Re-suspend cells in 1 mL of cell complete culture media.9. Count cells using Trypan Blue Solution and a Millicell® Disposable Hemocytometer. Mix 90% trypan bluewith 10% cell suspension (e.g., 180 µL trypan blue and 20 µL cell suspension).10. Count live cells on the hemocytometer and calculate cells per milliliter.11. Mix to create a single cell suspension and add desired cell suspension amount to 96-well microplate.Note: Millicell® ULA Plates can hold a maximum of 300 µL volume, but we advise <250 µL volume for best results.12. Depending on the cell type and medium, cells will aggregate to form a single spheroid within24-72 hours (about 3 days).How to Change MediumFor best results perform half media changes to avoid disrupting spheroid formation (if total well volume is 200 µL, then aspirate 100 µL and replace with fresh 100 µL volume).Millicell®Uniform spheroidformation resultsImaging and Staining SpheroidsSpheroids may be generated, cultured, and assayed for fluorescent or luminescent signals in the same Millicell® ULA Plate without the need to transfer them to another plate. The unique U-shaped well geometry makes them suitable for automated imaging of spheroids for high content screening approaches.Images below have been taken on the ImageXpress® Micro Confocal High-Content Imaging Systemwith 10x objective.Nuclear Actin CompositeA549HeLaHepG2MCF7In-well Assay Manipulation for Fixation and Staining Spheroid1. (x3) Remove 100 µL from supernatant and add100 µL wash buffer. Incubate at room temperature for 5 minutes.Note: For best results perform half media changes to avoid disrupting spheroid formation (if total well volume is 200 µL, then aspirate out 100 µL volume and replace with fresh 100 µL volume).2. Remove 100 µL from supernatant. Add in2x fixative (e.g., 8% PFA for a final concentration of 4% PFA) and incubate for 1 hour at 4 °C.Note: The fixative is made up as a 2x stock andadded 1:1 with the residual volume in the wellwith the spheroid.3. (x3) Remove 100 µL from supernatant and add100 µL wash buffer. Incubate at room temperature for 5 minutes.4. Remove 100 µL from supernatant. Add in primaryantibodies and incubate for 1 hour in the dark at room temperature.5. (x3) Remove 100 µL from supernatant and add100 µL wash buffer. Incubate at room temperature for 5 minutes.6. Remove 100 µL from supernatant. Add in secondaryantibodies and incubate for 1 hour in the dark atroom temperature.7. (x3) Remove 100 µL from supernatant and add100 µL wash buffer. Incubate at room temperature for 5 minutes.8. Remove 100 µL from supernatant. Dilute stock DAPIin PBS to 1 µg/µL and add 100 µL to each well.Incubate overnight in the dark at 4 °C.Note: Wrap your plate with paraffin or other sealant to avoid evaporation of liquid.9. (x3) Remove 100 µL from supernatant and add100 µL wash buffer. Incubate at room temperature for 5 minutes.10. Ready to image.TroubleshootingProblem Cause(s)Solution(s)Single cell suspensionaggregating/clumping Sticky/clumpy cells Use a 40 µm cell strainer to remove aggregates/clumps of cellsSpheroids not forming or multiple aggregates formed Pipette touched the bottom orsides of the microwell whenmanually seedingReseed plate avoiding contact with the platebottom or sidesULA coating damaged Replace the platePre-existing aggregates in well Start with a single cell suspensionMore time is requiredCells may take 24-72 hours depending ontype. Optimization is required for eachcell typeBacterial contamination Check culture for bacteria and use antibioticsin mediaSpheroids formingloose aggregates Cell lines may vary Fibroblast co-culture may be required Additional media supplementation such asmethyl-cellulose may be required Optimization is normally required for each cell typeLosing spheroids during media changes This is an ultra-low attachmentplate, non-adhesion spheroidsmight be inadvertently removedwith media aspiration.Perform half media changes andmicro-pipette gentlySlowly aspirate liquid so not to disturb3D culturesDo not set the pipette tip near the spheroidin the mediaSpheroids are not forming uniform size 3D aggregates Non-uniform starting single cellsuspensionEnsure that you are mixing the starting singlecell suspension before adding it to plate Heterogeneous co-culture cellmixtureMix co-culture cell types well beforedispensing into the plateUnknown starting quantity of cells Count cells in a hemocytometer to add aknown cell number to the plateUnequal media volume Add equivalent volumes of media to each wellThe life science business of Merck operates as MilliporeSigma in the U.S. and Canada.Merck, Millipore, Product Name, and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.© 2023 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.Document Template 00035533 Ver 1.0Product OrderingTo order , go to .DescriptionQuantity Catalogue Number Millicell Ultra-low Attachment Plates,96-well, U-bottom, Clear 20 pkMC96ULA20Trypan Blue Solution 50 mL 93595-50ML Trypsin100 mg T2600000Millicell ® Disposable Hemocytometer50 pkMDH-2N1-50PKNoticeWe provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose.The information in this document is subject to change without notice and should not be construed as acommitment by the manufacturing or selling entity, or an affiliate. We assume no responsibility for any errors that may appear in this document.Technical AssistanceVisit the tech service page on our web site at /TechService .Terms and Conditions of SaleWarranty, use restrictions, and other conditions of sale may be found at /Terms .Contact InformationFor the location of the office nearest you, go to /Offices .。
2安捷伦炼厂气分析仪助您可靠监测和优化催化及其他工艺安捷伦炼厂气分析仪 (RGAs) 基于 Agilent 8890 气相色谱系统。
每套系统均在出厂前经过配置和化学测试，可快速提供您所需的分析结果，同时节省您宝贵的启动时间。
您可以从扩展炼厂气、快速炼厂气、不凝气和烟道气分析的标准配置中进行选择，还可以基于 8890 气相色谱仪或 990 微型气相色谱仪定制炼厂气分析仪，以满足您的特定需求。
准确地分析炼厂气极具挑战性，因为各种气体样品的原料和组成差异很大。
为成功应对挑战，分析仪必须快速将各种炼厂和石化气体样品中的复杂混合物分离开来。
应用新型气相色谱技术，无需打乱应用工作流程Agilent 8890 气相色谱系统Agilent 990 微型气相色谱系统3安捷伦 RGAs 体现了行业标准和严格的质量控制流程每台分析仪都包括：出厂前–系统配置和泄漏检测–仪器校验 –安装合适的色谱柱–使用应用校验混标运行出厂前化学校验交付–运行方法所需的仪器操作手册 –含方法参数和校验数据文件的 DVD ，便于上手–包括消耗品，无需单独订购 –消耗品信息，以便再次订购安装–使用校验样品重复出厂校验，由经过厂家培训的支持工程师现场实施–可选的应用启动帮助智能互联气相色谱仪，与您同舟共济作为一款全新的气相色谱仪器，安捷伦NGAs 可自动监测系统健康状况、为您提醒潜在隐患，并助您随时解决问题。
这样您可以游刃有余地规划工作（包括维护），无需应对意外停机。
此外，该分析仪采用微流路电子气路控制模块 (EPC)。
这种安捷伦独有的设计可防止颗粒、水汽和油等污染物对 EPC 的损害，并提高仪器的可靠性和使用寿命。
最重要的是，您可以随时随地查看您的实验室。
移动访问功能让您可以随时查看设置信息、进行仪器诊断并解决故障、检查泄漏、实现色谱柱反吹功能、暂停和启动样品运行，并管理方法开发进程。
采用 Agilent 8890 快速 RGA 运行校验样品。
Technical BulletinACCUSPIN™ System – Histopaque ® 1077Catalog Numbers A6929, A7054, and A0561Product DescriptionACCUSPIN System-Histopaque -1077products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. The ACCUSPIN tube is specially designed with two chambers separated by a porous high density polyethylene barrier (frit).Separation of lymphocytes and other mononuclear cells from whole blood and bone marrow using density gradientseparation media is based on a published method.1 Histopaque-1077 is suitable for human lymphocyte antigen (HLA) typing 2 and as the initial isolation step prior toenumeration of T, B, and ‘null’ lymphocytes.3 It may also be employed in the preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.4ACCUSPIN System-Histopaque-1077 products consist of radiation sterilized polypropylene tubes fitted with a highdensity polyethylene frit and aseptically filled with Histopaque-1077.Histopaque-1077 is a sterile-filtered solution of polysucrose, 57 g/L, and sodium diatrizoate, 90 g/L.Density: 1.076–1.078 g/mL Endotoxin: 0.3 EU/mL pH: 8.8–9.0ACCUSPIN System-Histopaque-1077Catalog No. A692940 × 3 mLEach tube contains 3 mL ofHistopaque 1077-1 and will separate 3-6 mL of anticoagulated blood Catalog No. A7054 12 × 15 mLCatalog No. A0561100 × 15 mLEach tube contains 15 mL ofHistopaque 1077-1 and will separate 15-30 mL of anticoagulated bloodReagents and Equipment Required but Not ProvidedCentrifuge (swinging bucket rotor)capable of generating 100 to 1,000 g Centrifuge tubes for washing mononuclear cellsIsotonic phosphate buffered saline solution or appropriate cell culture mediumPrecautions and DisclaimerFor R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.Preparation InstructionsSpecimen Collection - Collect blood in preservative-free anticoagulant (EDTA or heparin) or use defibrinated blood. For best results, blood should be processed within 2 hours.On occasion, it may be necessary to dilute the blood sample 3 to 5-fold, depending on absolute cell numbers. A similar volume of prediluted blood may be used or the blood sample may be diluted directly in upper chamber of the ACCUSPIN tube (seeProcedure, step 3). This is appropriate for specimens with hematocrits above normal.Storage/StabilityStore the products at 2–8 C.Histopaque-1077 has an expiration period of 3 years. Reagent label bears expiration date.ProcedureAnticoagulated blood can be added to the top chamber of the tube without risk of mixing with the Histopaque-1077 in the lowerchamber under the frit. On centrifugation the whole blood migrates through the frit to contact with the Histopaque-1077. The elements of greater density displace a volume of Histopaque-1077 above the frit giving a clear separation of the bloodcomponents. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN Tube. Lymphocytes and other mononuclear cells, e.g., monocytes, remain at the plasma/Histopaque-1077 interface. This dense band of mononuclear cells may be collected by pouring off the contents of the upper chamber or by means of a pipette. Erythrocyte contamination is avoided due to the barrier between the chambers.Most extraneous platelets are removed by low speed centrifugation during the washing steps.1. Bring desired number of tubes to roomtemperature. If Histopaque-1077 isabove the frit prior to use, centrifuge at 1,000 g for 30 seconds at room temperature.Note: Failure to bring ACCUSPIN System-Histopaque-1077 to room temperature may cause limited recovery of mononuclear cells. 2. Label tube(s).3. Freely pour the blood sample into theupper chamber of each ACCUSPIN System-Histopaque-1077 tube.a. Use 3–6 mL of whole blood withACCUSPIN System-Histopaque-1077 tubes, Catalog No. A6929. b. Use 15–30 mL of whole blood withACCUSPIN System-Histopaque-1077 tubes, Catalog Nos. A7054 or A0561. Note: Use of volumes of prediluted or whole blood other than those recommended may result in decreased recovery.4. Centrifuge at 1,000 g for 10 minutes atroom temperature or centrifuge at 800 g for 15 minutes at roomtemperature. Centrifugation at lower temperatures, such as 4 C, may result in cell clumping and poor recovery.Note: If platelet contamination is a concern, add the mononuclear cells to a 4-20% sucrose gradient that has been layered over Histopaque-1077.Centrifuge at 1,000 × g for 10 minutes at room temperature. The platelets will pellet at the bottom, while themononuclear cells will migrate to the Histopaque-1077 layer.5. After centrifugation, carefully aspiratethe plasma layer with a Pasteur pipette to within 0.5 cm of the opaque interface containing mononuclear cells. Properly dispose of the plasma layer.Note: Failure to remove the excesssupernatant may result in contamination of the mononuclear band with plasma proteins.6. Carefully transfer the opaque interfacewith a Pasteur pipette into a clean conical centrifuge tube.Note: Removal of Histopaque-1077 with the mononuclear band increasesgranulocyte contamination from residual granulocytes, which may remain at the mononuclear interface.7. Wash the cells by adding 10 mL ofisotonic phosphate buffered saline solution or appropriate cell culture medium and mix by gently drawing in and out of a Pasteur pipette. 8. Centrifuge at 250 g for 10 minutes. 9. Aspirate the supernatant and discard. 10. Resuspend cell pellet with 5 mL ofisotonic phosphate buffered saline solution or appropriate cell culture medium and mix by gently drawing in and out of a Pasteur pipette.11. Centrifuge at 250 g for 10 minutes. 12. Repeat steps 9, 10, and 11, discardsupernatant and resuspend cell pellet in 0.5 mL of isotonic phosphate buffered saline solution or appropriate cell culture medium. Erythrocytes and granulocytes should pellet to the bottom of the ACCUSPIN tube. Mononuclear cells should band at the interface between the Histopaque-1077 and the plasma. If observed results vary from expected results, please contact Sigma-Aldrich Technical Service for assistance.References1. Boyum, A., Separation of leukocytesfrom blood and bone marrow. Scand. J. Clin. Lab. Invest ., 21 (Suppl 97), 77 (1968).2. Amos, D.B., and Pool, P., “HLA typing” inManual of Clinical Immunology, Rose, N.R., and Friedman, H., eds., American Society for Microbiology, (Washington, DC: 1976) pp. 797-804.3. Winchester, R.J., and Ross, G., “Methodsfor enumerating lymphocyte populations” in Manual of Clinical Immunology, Rose, N.R., and Friedman, H. eds., American Society for Microbiology, (Washington, DC: 1976) pp. 64-76.4. Thorsby, E., and Bratlie, A., “A rapidmethod for preparation of pure lymphocyte suspensions.”Histocompatibility Testing, Terasaki, P.I., ed., 665-666 (1970).The life science business of Merck operates as MilliporeSigma in the U.S. and Canada.Merck, Sigma-Aldrich, ACCUSPIN, and Histopaque are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.© 2022 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.NoticeWe provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose.The information in this document is subject to change without notice and should not be construed as a commitment by the manufacturing or selling entity, or an affiliate. We assume no responsibility for any errors that may appear in this document.Contact InformationFor the location of the office nearest you, go to /offices .Technical ServiceVisit the tech service page on our web site at /techservice .Standard WarrantyThe applicable warranty for the products listed in this publication may be found at /terms .A0561 Technical Bulletin Rev 06/2022。
专利名称：一种全自动样品前处理仪专利类型：实用新型专利
发明人：孙敦元
申请号：CN202122032417.4
申请日：20210826
公开号：CN215728205U
公开日：
20220201
专利内容由知识产权出版社提供
摘要：本实用新型公开了一种全自动样品前处理仪，包括底板，所述底板的上表面固定连接有进样臂，进样臂的输出端固定连接有旋转横梁，旋转横梁的一端卡接有进样针，所述底板的上表面固定连接有试剂仓，试剂仓的两侧内壁一体成型有多个卡块，且试剂仓的一侧固定连接有进样针清洗池，所述底板的上表面活动连接有样品加热盘，样品加热盘内分别设有多个样品管槽和多个氮吹管槽，所述底板的上表面固定连接有氮吹臂，氮吹臂的输出端固定连接有环形氮吹针，环形氮吹针的下表面设有多个吹气针，吹气针均匀分布，且吹气针与氮吹管槽相对应。
本实用新型代替了人工操作，实现了全自动化，提升了样品处理效率，保障了样本的后期检验结果。
申请人：南京劳拉苏埃尔电子有限公司
地址：210000 江苏省南京市秦淮区光华路1号高新技术产业园C2
国籍：CN
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Page:1/14SAFETY DATA SHEETFOR INDUSTRIAL USE ONLYE828RS /A100 500LB CLOSED HEADSection 1. Product and company identificationGHS product identifier : E828RS /A100 500LB CLOSED HEADMSDS Number : K122FProduct type : Epoxy ResinMaterial uses : Epoxy Resin SystemsManufacturer/Supplier/Importer:Westlake Epoxy Inc.12650 DIRECTORS DR STE 100Stafford, Texas 77477USAContact person:Telephone:For additional health and safety or regulatory information, call Emergency telephone number:For Emergency Medical AssistanceCall Health & Safety Information Services1-866-303-6949For Emergency Transportation InformationNCEC US Domestic +1 866 928 0789 (toll-free, US only)NCEC Americas +1 215 207 0061CANUTEC CA Domestic (613) 996-6666Section 2. Hazards identificationClassification of the substance or mixture : SKIN IRRITATION - Category 2 EYE IRRITATION - Category 2ASKIN SENSITISATION - Category 1GHS label elementsHazard pictograms:Signal word: WarningHazard statements : H315 Causes skin irritation.H319 Causes serious eye irritation.H317 May cause an allergic skin reaction. Precautionary statementsGeneral: Not applicable.Prevention: Wear eye or face protection.Avoid breathing vapor.Wash thoroughly after handling.Response : Take off contaminated clothing and wash it before reuse.Wash contaminated clothing before reuse.IF ON SKIN:Wash with plenty of water.IF IN EYES:Rinse cautiously with water for several minutes. Remove contactlenses, if present and easy to do. Continue rinsing.If eye irritation persists:Get medical advice or attention.Storage: Not applicable.Disposal: Dispose of contents and container in accordance with all local,regional, national and international regulations.: None known.Other hazards which do not resultin classificationSubstance/mixture : MixtureAn alternate description is CAS number 1675-54-3Any concentration shown as a range is to protect confidentiality or is due to batch variation.There are no additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section.Occupational exposure limits, if available, are listed in Section 8.Description of necessary first aid measuresEye contact: Immediately flush eyes with plenty of water, occasionally lifting theupper and lower eyelids. Check for and remove any contact lenses.Continue to rinse for at least 10 minutes. Get medical attention. Inhalation : Remove victim to fresh air and keep at rest in a position comfortablefor breathing. If not breathing, if breathing is irregular or if respiratoryarrest occurs, provide artificial respiration or oxygen by trainedpersonnel. It may be dangerous to the person providing aid to givemouth-to-mouth resuscitation. Get medical attention if adverse healtheffects persist or are severe. If unconscious, place in recovery positionand get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.Skin contact: Wash with plenty of soap and water. Remove contaminated clothingand shoes. Wash contaminated clothing thoroughly with water beforeremoving it, or wear gloves. Continue to rinse for at least 10 minutes.Get medical attention. In the event of any complaints or symptoms,avoid further exposure. Wash clothing before reuse. Clean shoesthoroughly before reuse.Ingestion: Wash out mouth with water. Remove dentures if any. Remove victimto fresh air and keep at rest in a position comfortable for breathing. Ifmaterial has been swallowed and the exposed person is conscious, givesmall quantities of water to drink. Stop if the exposed person feels sickas vomiting may be dangerous. Do not induce vomiting unless directedto do so by medical personnel. If vomiting occurs, the head should bekept low so that vomit does not enter the lungs. Get medical attentionif adverse health effects persist or are severe. Never give anything bymouth to an unconscious person. If unconscious, place in recoveryposition and get medical attention immediately. Maintain an openairway. Loosen tight clothing such as a collar, tie, belt or waistband. Indication of immediate medical attention and special treatment needed, if necessaryNotes to physician: Treat symptomatically. Contact poison treatment specialistimmediately if large quantities have been ingested or inhaled. Specific treatments: No specific treatment.Protection of first aid personnel: No action shall be taken involving any personal risk or withoutsuitable training. It may be dangerous to the person providing aid togive mouth-to-mouth resuscitation. Wash contaminated clothingthoroughly with water before removing it, or wear gloves.See toxicological information (Section 11)Extinguishing mediaSuitable extinguishing media: Use an extinguishing agent suitable for the surrounding fire. Unsuitable extinguishing media : None known.Specific hazards arising from the chemical : In a fire or if heated, a pressure increase will occur and the container may burst.Hazardous thermal decomposition products : Decomposition products may include the following materials: carbon dioxidecarbon monoxidehalogenated compoundsSpecial protective actions for fire-fighters : Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.Special protective equipment for fire-fighters : Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.Personal precautions, protective equipment and emergency proceduresFor non-emergency personnel: No action shall be taken involving any personal risk or withoutsuitable training. Evacuate surrounding areas. Keep unnecessary andunprotected personnel from entering. Do not touch or walk throughspilled material. Avoid breathing vapor or mist. Provide adequateventilation. Wear appropriate respirator when ventilation isinadequate. Put on appropriate personal protective equipment.For emergency responders : If specialised clothing is required to deal with the spillage, take note ofany information in Section 8 on suitable and unsuitable materials. Seealso the information in "For non-emergency personnel". Environmental precautions: Avoid dispersal of spilled material and runoff and contact with soil,waterways, drains and sewers. Inform the relevant authorities if theproduct has caused environmental pollution (sewers, waterways, soilor air).Methods and material for containment and cleaning upSmall spill: Stop leak if without risk. Move containers from spill area. Dilute withwater and mop up if water-soluble. Alternatively, or if water-insoluble,absorb with an inert dry material and place in an appropriate wastedisposal container. Dispose of via a licensed waste disposal contractor.Large spill: Stop leak if without risk. Move containers from spill area. Approachrelease from upwind. Prevent entry into sewers, water courses,basements or confined areas. Wash spillages into an effluent treatmentplant or proceed as follows. Contain and collect spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite ordiatomaceous earth and place in container for disposal according tolocal regulations (see section 13 of SDS). Dispose of via a licensedwaste disposal contractor. Contaminated absorbent material may posethe same hazard as the spilled product. Note: see section 1 of SDS foremergency contact information and section 13 of SDS for wastedisposal.Precautions for safe handlingProtective measures: Put on appropriate personal protective equipment (see section 8 ofSDS). Persons with a history of skin sensitization problems should notbe employed in any process in which this product is used. Do not getin eyes or on skin or clothing. Do not ingest. Avoid breathing vapor ormist. Keep in the original container or an approved alternative madefrom a compatible material, kept tightly closed when not in use.Empty containers retain product residue and can be hazardous. Do notreuse container.Advice on general occupational hygiene : Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. Seealso Section 8 for additional information on hygiene measures.Conditions for safe storage, : Store in accordance with local regulations. Store in original containerincluding any incompatibilities protected from direct sunlight in a dry, cool and well-ventilated area,away from incompatible materials (see section 10 of SDS) and foodand drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and keptupright to prevent leakage. Do not store in unlabeled containers. Useappropriate containment to avoid environmental contamination.Control parametersOccupational exposure limitsRecommended monitoring procedures : If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required todetermine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Reference should be made to appropriate monitoring standards. Reference tonational guidance documents for methods for the determination ofhazardous substances will also be required.If this product containsingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Reference should be made toappropriate monitoring standards. Reference to national guidancedocuments for methods for the determination of hazardous substances will also be required.Appropriate engineering controls: No special ventilation requirements. Good general ventilation shouldbe sufficient to control worker exposure to airborne contaminants. Ifthis product contains ingredients with exposure limits, use processenclosures, local exhaust ventilation or other engineering controls tokeep worker exposure below any recommended or statutory limits. Environmental exposure controls : Emissions from ventilation or work process equipment should bechecked to ensure they comply with the requirements of environmentalprotection legislation. In some cases, fume scrubbers, filters orengineering modifications to the process equipment will be necessaryto reduce emissions to acceptable levels.Individual protection measuresHygiene measures : Wash hands, forearms and face thoroughly after handling chemicalproducts, before eating, smoking and using the lavatory and at the endof the working period. Appropriate techniques should be used toremove potentially contaminated clothing. Contaminated workclothing should not be allowed out of the workplace. Washcontaminated clothing before reusing. Ensure that eyewash stationsand safety showers are close to the workstation location.Eye/face protection: Safety eyewear complying with an approved standard should be usedwhen a risk assessment indicates this is necessary to avoid exposure toliquid splashes, mists, gases or dusts. If contact is possible, thefollowing protection should be worn, unless the assessment indicates ahigher degree of protection: chemical splash goggles.Skin protectionHand protection: Chemical-resistant, impervious gloves complying with an approvedstandard should be worn at all times when handling chemical productsif a risk assessment indicates this is necessary. Considering theparameters specified by the glove manufacturer, check during use thatthe gloves are still retaining their protective properties. It should benoted that the time to breakthrough for any glove material may bedifferent for different glove manufacturers. In the case of mixtures,consisting of several substances, the protection time of the glovescannot be accurately estimated.Body protection : Personal protective equipment for the body should be selected basedon the task being performed and the risks involved and should beapproved by a specialist before handling this product.Other skin protection : Appropriate footwear and any additional skin protection measuresshould be selected based on the task being performed and the risksinvolved and should be approved by a specialist before handling thisproduct.Respiratory protection : Based on the hazard and potential for exposure, select a respirator thatmeets the appropriate standard or certification. Respirators must beused according to a respiratory protection program to ensure properfitting, training, and other important aspects of use.AppearancePhysical state : Viscous liquid.Color: Clear.Odor: Not availableOdor threshold: Not availablepH: Not availableMelting point/ Freezing point: Not availableBoiling point: 260 °C (500 °F)Flash point : Pensky-Martens Closed Cup: 251 °C (484 °F) (ASTM D 93) Burning time : Not availableBurning rate : Not availableEvaporation rate : Not availableFlammability (solid, gas): Not availableLower and upper explosive (flammable) limits : Lower: Not available Upper: Not availableVapor pressure : 0.03 mbar @ 77 °C (171 °F) Vapor density: Not availableRelative density: 1.17 Solubility: Not available Solubility in water : NegligiblePartition coefficient: n-octanol/water : Not availableNot applicable.Auto-ignition temperature : Not availableDecomposition temperature: Not availableSADT: Not availableViscosity: Dynamic: Not availableKinematic: Not availableOther informationNo additional information.Reactivity: Stable under normal conditions.Chemical stability : The product is stable.Possibility of hazardous reactions: Under normal conditions of storage and use, hazardous reactions willnot occur.Conditions to avoid : No specific data.Incompatible materials : No specific data.Hazardous decomposition products : Under normal conditions of storage and use, hazardous decompositionproducts should not be produced.Other hazards Reacts with considerable heat release with some curing agents.Information on toxicological effectsAcute toxicityIrritation/CorrosionSkin: Not available eyes: Not available Respiratory: Not available SensitizationSkin: Not available Respiratory: Not available MutagenicityCarcinogenicityConclusion/Summary: Not availableReproductive toxicityConclusion/Summary: Not availableTeratogenicityConclusion/Summary: Not availableSpecific target organ toxicity (single exposure)Not availableSpecific target organ toxicity (repeated exposure)Not availableAspiration hazardNot available: Not availableInformation on likely routes ofexposurePotential acute health effectsEye contact : Causes serious eye irritation.Inhalation : No known significant effects or critical hazards.Skin contact: Causes skin irritation. May cause an allergic skin reaction. Ingestion: No known significant effects or critical hazards. Symptoms related to the physical, chemical and toxicological characteristicsEye contact : Adverse symptoms may include the following:pain or irritationwateringrednessInhalation : No specific data.Skin contact : Adverse symptoms may include the following:irritationrednessIngestion: No specific data.Delayed and immediate effects as well as chronic effects from short and long-term exposureShort term exposurePotential immediate effects: Not availablePotential delayed effects : Not availableLong term exposurePotential immediate effects: Not availablePotential delayed effects : Not availablePotential chronic health effectsConclusion/Summary: Not availableGeneral : Once sensitized, a severe allergic reaction may occur whensubsequently exposed to very low levels. Carcinogenicity: No known significant effects or critical hazards. Mutagenicity : No known significant effects or critical hazards. Teratogenicity : No known significant effects or critical hazards. Developmental effects: No known significant effects or critical hazards.Fertility effects: No known significant effects or critical hazards. Numerical measures of toxicityAcute toxicity estimatesToxicityConclusion/Summary: Not availablePersistence/degradabilityConclusion/Summary: Not availableBioaccumulative potentialMobility in soilSoil/water partition coefficient(KOC): Not availableOther adverse effects : No known significant effects or critical hazards.Disposal methods : The generation of waste should be avoided or minimized whereverpossible. Disposal of this product, solutions and any by-productsshould at all times comply with the requirements of environmentalprotection and waste disposal legislation and any regional localauthority requirements. Dispose of surplus and non-recyclableproducts via a licensed waste disposal contractor. Waste should not bedisposed of untreated to the sewer unless fully compliant with therequirements of all authorities with jurisdiction. Waste packagingshould be recycled. Incineration or landfill should only be consideredwhen recycling is not feasible. This material and its container must bedisposed of in a safe way. Care should be taken when handlingemptied containers that have not been cleaned or rinsed out. Emptycontainers or liners may retain some product residues. Avoid dispersalof spilled material and runoff and contact with soil, waterways, drainsand sewers.The data provided in this section is for information only and may not be specific to your package size ormode of transport. You will need to apply the appropriate regulations to properly classify your shipment for transportation.International transport regulationsRegulatory information UN/NAnumberProper shipping name Classes/*PG ReportableQuantity (RQ)CFR Non-regulated TDG Non-regulatedClass 9 IIIIMO/IMDG3082ENVIRONMENTALLYHAZARDOUS SUBSTANCE,LIQUID, N.O.S.(LIQUID EPOXY RESIN)Class 9 IIIIATA (Cargo)3082ENVIRONMENTALLYHAZARDOUS SUBSTANCE,LIQUID, N.O.S.(LIQUID EPOXY RESIN)*PG : Packing groupEnvironmentally hazardous and/or Marine Pollutant :Yes.Special precautions for user: Transport within user’s premises: always transport in closedcontainers that are upright and secure. Ensure that personstransporting the product know what to do in the event of an accidentor spillage.Section 15. Regulatory informationUnited StatesU.S. Federal regulations :United States - TSCA 12(b) - Chemical export notification: Nonerequired.United States - TSCA 5α2 - Final significant new use rules: Not listedUnited States - TSCA 5α2 - Proposed significant new use rules: NotlistedUnited States - TSCA 5(e) - Substances consent order: Not listedSARA 311/312 Classification - SKIN IRRITATION, Category 2SARA 311/312 Classification - EYE IRRITATION, Category 2ASARA 311/312 Classification - SKIN SENSITISATION, Category 1SARA 311/312 Classification - Not applicableCalifornia Prop. 65:WARNING: This product may contain one or more chemicals known to the state of California to cause cancer, birth defects or other reproductive harm.: All components are active or exempted.United States inventory (TSCA8b)International regulationsInternational lists : Canada inventory: All components are listed or exempted.China inventory (IECSC): All components are listed or exempted.Korea inventory (KECI): All components are listed or exempted.New Zealand Inventory (NZIoC): All components are listed or exempted.Philippines inventory (PICCS): All components are listed or exempted.United States inventory (TSCA 8b): All components are active or exempted.Taiwan inventory (TCSI): All components are listed or exempted.representing significant hazards or risks Although HMIS® ratings are not required on MSDSs under 29 CFR 1910.1200, the preparer may choose to provide them. HMIS® ratings are to be used with a fully implemented HMIS® program. HMIS® is a registered mark of the National Paint & Coatings Association (NPCA). HMIS®materials may be purchased exclusively from J. J. Keller (800) 327-6868.The customer is responsible for determining the PPE code for this material. For more information on HMIS®Personal Protective Equipment (PPE) codes, consult the HMIS® Implementation Manual.: Not applicable.Full text of abbreviated HstatementsHistoryDate of printing : 01/30/2023Date of issue/Date of revision: 09/21/2022Date of previous issue : 03/30/2022Version: 21.0Prepared by : Product Safety StewardshipKey to abbreviations : ATE = Acute Toxicity EstimateBCF = Bioconcentration FactorGHS = Globally Harmonized System of Classification and Labelling of ChemicalsIATA = International Air Transport AssociationIBC = Intermediate Bulk ContainerIMDG = International Maritime Dangerous GoodsLogPow = logarithm of the octanol/water partition coefficientMARPOL = International Convention for the Prevention of Pollution From Ships, 1973as modified by the Protocol of 1978. ("Marpol" = marine pollution)RID = The Regulations concerning the International Carriage of Dangerous Goods byRailUN = United NationsReferences : Not availableNotice to readerThe information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text.。
C-29OMEGA’s LAbOrAtOry-GrAdEbEnchtOp Wind tunnELU P recise Motor Control for Adjusting Flowrates U N IST Calibration Supplied with Each Wind Tunnel U H ighly Accurate to 1% of Reading U L arge 101.6 x 101.6 mm (4 x 4") Test Chamber U H ighly Stable Delta Pressure Sensor and Readout Included with Each Wind Tunnel U Fully Instrumented U O ptional Environmental Monitoring Package of Temp, Humidity, Barometric Pressure and Delta P AvailableWT4401-DThe uniform flowrate is determined by monitoring a highly repeatabledifferential pressure sensor which has been calibrated to each individual wind tunnel as a system. Each wind tunnel is supplied with two restrictive plates for achieving optimum low flowrates. The established differential pressure measurements versus flowrates are listed from 25 to 9000 FPM. Calibration sheets are included, which makescalibrating different flow sensors simple.The differential pressure measurementused to establish known flowrates will be affected by barometric pressure and temperature conditions during testing. Depending on the probe being calibrated, humidity may also be a factor.OMEGA’s deluxe wind tunnel package is an environmental monitoring system that measures barometric pressure, room temperature, humidity and differential pressure. By monitoring room conditions, standard differential pressures can be converted to actual differential pressure readings to ensure accurate flowrates.For testing different probes, a ¹⁄₂ NPT connection is available. An assortment of compression fittings is provided to help mount the probe under calibration.OMEGA’s state-of-the-art wind tunnel is designed to give a highly uniform flow rate over a 152 mm (6") testsection. A powerful 12 amp motor with variable speed from 0 to 10,000 RPM is adjustable to give a particular flowrate by a precise motor control unit. Each wind tunnel is supplied with an NIST traceable certificate.SPECIFICATIONSWind TunnelAccuracy: 1% rdg(2% rdg with restrictive plates)Flow Rates: 25 to 9000 fpm (0.15 to 45 m/s)Noise: 95 dBA maximumSize: 1.5 m L x 431.8 mm H (5' x 17")MotorPower: 120 Vac Current: 12 amps Speed: 10,000 RPMMotor Control BoxPower: 120 VacSpeed Adjustment: 10 turn pot.Internal Adjustment: Minimum, Maximum, acceleration and current limit Size: 140 W x 184 L x 89 mm H (5.53 x 7.25 x 3.5")Weight: Wind tunnel: 22.5 kg (49.5 lb); Standard elec. 3.2 kg (7 lb); Deluxe elec. 7 kg (15.3 lb)Monitoring SystemsStandard Package:PX657 127 mm (5") H 2O differentialpressure sensor and DP41-S-A meter with analog output in benchtop caseWT4401-D includes deluxe electronics package formeasuring temperature, humidity and barometric and differentialpressures.Shown calibrating the FMA-902A-V1, sold separately, for more information visit us online.package have a 10 Vdc analog output.Deluxe Package:Same differential system as standard model, plus HX97-A temperature and humidity sensor, PX02C1-16A5T barometric sensor, PSS-D15B power supply, three DP25-E panel meters, and one DP41B-S panel meter. All equipment is enclosed in a heavy duty, air cooled, benchtop metal case. All 4 meters have 0 to 10 Vdc analog outputs.Wind tunnels come complete with 1⁄8, 3⁄16, 1⁄4 and 3⁄8" brasscompressionfittings withPTFE ferrules, two 1.52 m (5') lengths of silicone tubing, operator’s manual with NIST calibration data and pressure sensor with benchtop meter.The intake air flow passage to this precise wind tunnel is a fiberglass- constructed baffle. The honeycomb baffle design insures constant andevenly distributed air intake. The design minimizes turbulent air flow created by movement in front of the intake. Therear portionof the intakebaffle has a profile design that keeps the stream uniform, while decreasing the area to a 101.6 x 101.6 mm (4 x 4")cross- section.The test chamber is 101.6 x 101.6 x 152 mm(4 x 4 x 6") and is constructedof clear, thick-wall plastic for easy viewing. In back of this test section, the motor pulls the air through the wind tunnel. The motor and intake profilesC-30Cutilize fiberglass construction, which minimizes airflow distortion.A deluxe monitoring package keeps track of ambient conditions as well as the precise differential pressure measurement. The monitoring sensors and instruments are all packaged inside a single powerful housing. Each sensor has an analog output for use with a strip chart recorder or computer data logging system. Humidity, temperature and barometric sensors are enclosed in the benchtop case for monitoring atmospheric conditions which can influence the exact air speed. Visit for the complete listing of all accessories included.For 220 Vac, add suffix “-220V” to model number, no additional charge.Ordering Examples: WT4401-D, deluxe wind tunnel package, SA2C-K Type Kmolded silicon self adhesive thermocouple, and FMA-902A-V1, air velocity transducer. WT4401, standard wind tunnel package with HHF42 hotwire anemometer.WT4401 rear WT4401 front baffle s t a n d a r da n d d e l u x ep a c k a ge s E s t a b l i s h F l o w r a t e sf r o m25 t o 9000 F p M The differential pressure cell isconnected to the wind tunnel via silicone tubing. The entrance and test chambers’ pressures are monitored, which determines true air speed.。
杭州美仪自动化有限公司杭州美仪自动化有限公司U-SUP-PH6.0-MYCN 5第5版酸碱度氧化还原在线分析仪使用说明书前言●感谢您购买本公司产品。
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True to the Music ®True to the Music®User GuideP HA ZE Q ™500 T OOL Phase Alignment Tool & Filter500 Series ModuleRadial Engineering Ltd.1588 Kebet Way, Port CoquitlamBritish Columbia, Canada, V3C 5M5tel: 604-942-1001 • fax: 604-942-1010Radial® P HAZE Q™ 500 User GuideTable of Contents ......................................................PageFeature Set (1)Overview (2)Getting Started (4)Using As Phase Adjustment (6)Using As Equalizer (7)Using The Low-pass Filter (8)Specifi cations (9)Block Diagram (9)Warranty ...........................................................Back coverCongratulations and thank you. You are now the proud owner of the Radial PhaseQ™ 500, one of the most intriguing products we have ever developed. Why intriguing you ask? Well, simply stated, messing around with phase is kind of like bending time. Mother Nature really gets upset when we do these things! And to add even more confusion, we are not merely messing with the phase between two microphones, the PhaseQ can actually be used as a whacky equalizer! So hold onto your hat… this ride will be fun.To get you there as safely as possible, we recommend that you take the time to read this manual. This will give you some insight on how to use the PhaseQ and what is actually going on when you move the dials. If by chance you do not fi nd all of the answers you are looking for, please visit the FAQ page at . This is where we post the latest bits and pieces of information. Then, if you don’t fi nd what your are looking for there send us an *************************************************************************. Fasten your seatbelts, set the time-bend constant to year 3022... and then close your eyes. Listen. Reality just got a whole lot more exciting!True to the Music ®FEATURE SET1. BLEND – Controls the mix between theoriginal (dry) signal and the phase altered(wet) signal.2. PHASE SHI FT – Used to set the phaserelationship between the dry and wetsignals or between two devices whenusing a stereo source.3. I NVERT - 180º polarity reverse shiftsthe phase shift range from 0º to 180º to181º to 360º for control over the completefrequency cycle.4. ON (phase shift) – Used to bypass thephase shift section and compare theoriginal sound with the phase alteredsignal.5. F LTER – Sets the high frequency cutoff range of the low-pass fi lter. Thislets you apply the phase effect to lowerfrequencies.6. SHIFT - Changes the fi lter range from thehigh range (3kHz to 38kHz) to the lowrange (300Hz to 3.8kHz)7. ON (fi lter) – Used to bypass the fi ltersection and compare the processedsignal with the original.8. OMNI PORT - Available when used withthe Radial Workhorse, the Omniport isassigned as a direct dry signal output forre-patching to other devices.®True to the Music OVERVIEWThe PhazeQ can be considered as two different devices in one. I ts primary task is to provide added realism by allowing two sources to be brought together in such a way that their fundamental frequencies combine in harmony. Analogue phase control should not be measured… you must listen and decide. The other side of the PhazeQ is destructive creativity. In other words, we invest all kinds of time and energy trying to ensure the audio signals are perfectly phase-aligned and then… the PhazeQ lets you completely ruin this by allowing you to introduce radical sounding EQ curves via phase cancellation. It truly is a case of good and bad in one box.Phase Alignment - The good!When two microphones are placed in a room, depending on where they are positioned with respect to the source, the sound will inevitably arrive at a different time as it travels through the air. When the two microphone signals are mixed together they will inevitably be out of phase. This causes an effect known as comb-fi ltering.TimeClose Mic Distant MicBy altering the phase (or delaying the sound) of the nearest microphone, you can shift the fundamental frequency so that it will more gracefully combine with the sound being captured by the second microphone.What is surprising is that this effect is not merely intended for mics that are broadly spaced, but the effect is just as astounding on microphones that are close together such as an XY confi guration. The effect is even more pronounced when combining a direct signal such as from a direct box with a room microphone. Engineers will often spend hours moving mics to capture the perfect tone. The PhazeQ lets you quickly do it by turning a dial.True to the Music ®PhazeQ as an EQ - The Bad!As soon as you change the EQ setting on your mixer, you are in fact messing with the phase. This is the nature of the beast. Most EQ circuits are designed to minimize phase shift for this very purpose. In fact, EQs like the Radial Q3 Induction Coil EQ are sought after for this reason. They introduce less phase alterations.Signal Split Dry Signal(unaffected)What happens here is that you can cancel or augment certain frequencies to create radicalTrue to the Music ®GETTING STARTEDMaking ConnectionsBefore making any connections, start by turning off your audio system and turning all volume levels down. This helps protect equipment from turn-on transients that could damage loudspeakers and other sensitive equipment. We recommend using a power bar with an on-off switch as this makes it easy to turn on and off the 500 series rack, monitors and so on, using a single switch. Carefully plug the PhazeQ into your 500 series rack avoiding stress on the card edge connector. Screw the module in to ensure it does not accidentally become dislodged.Connections between the PhazeQ and the recording or PA system are made at the rear panel of your 500 series rack. Most 500 series racks are equipped with XLR connectors. When you plug the PhazeQ module into your 500 series rack, it will automatically route the card-slot input and output XLR jacks to the module. With the Radial Workhorse 500 series rack the I/O is augmented with ¼” TRS connectors, D-Subs and the Mix Buss signal to feedthe Workhorse mixer.Start by setting up the PhazeQ panel controls as follows:1. BLEND: set to the 50% position (12 o’clock)2. PHASE SHIFT: set to zero degrees (7 o’clock)3. INVERT: outward position (0° ~ 180°)4. ON: outward position (PhaseQ bypassed)5. FILTER: set to fully clockwise6. RANGE: outward position (300Hz ~ 3.8kHz)7. ON: outward position (fi lter bypassed)True to the Music ®The PhazeQ is a professional line level device designed to be used with the Radial Workhorse or other 500 series rack. This means that it is intended to process signals from +4dB balanced line sources such as the output from a recording system, mixing console or mic preamplifier.PowerPre 500Mic Preamp PhazeQ 500ModuleLine Line MicConnect the source signal to the PhazeQ by plugging into the XLR male input of your 500-series rear panel. If you have a Workhorse, the channel to the left can also supply a signal to the PhazeQ via the FEED connection. Connect the XLR output to the recorder.The PhazeQ can be used as either a phase adjustment tool or as an equalizer. The difference between the two is simple: when phase adjusting, you are basically bringing two different sources into phase while as an EQ, you are taking one source, splitting it in two and then recombining to create an effect.PHASE ADJUSTMENT: This setup shows how one microphone’s signal is phase shifted comparedto the other. The two signals are combined together at the mixing console or recorded to one track.EQ AND TONE SHAPING: With a single microphone the BLEND control is used to mix the originaland phase shifted signals together. This combined with the LPF fi lter can create a variable EQ thatuses phase cancellation to shape the sound.True to the Music ®Using The PhazeQ As A Phase Adjustment ToolWhen recording, two mics are often combined to capture different parts of the instrument. For example, on a guitar you may want to pick up sound from both the hole and the bridge. On a piano, you may have one mic capturing the bass registers while a second mic captures the highs. Spill from the instrument will invariably feed both microphones. This means that some frequencies, when combined will be in phase and therefore reinforce each other and become louder, while some will cancel each other out and be attenuated. Moving the microphones around will change the sound.This happens because each frequency has a different wavelength and the relationship between the many frequencies will cause them to combine or cancel depending on their relative phase. Engineers will often take hours moving mics around the room trying to fi nd the ideal mic positions. The PhazeQ is basically an electronic device that does the same thing: it lets you move the mic around electronically with surgical precision. What it will not do is fi x the relationship between the two sources. This is impossible as each frequency has a different wavelength and they go on forever. With a PhazeQ, you will actually have to use your ears and listen to fi nd the sweet spot that works.A common set up would be to load your 500 series rack with two PowerPre mic preamps and a PhazeQ. You would connect each microphone to a preamp and feed the signal from the preamp that is closest to the source into the PhazeQ. Both outputs would then feed to separate channels on your recording system. Set the BLEND control to wet, the phase adjustment counter-clockwise, and make sure the low-pass fi lter is off. Bring up the level on the direct (far) mic to make sure it is working. Then, bring up the level one the ‘mic to PhazeQ` channel (near). Set both channels to about the same level, turn on the phaseadjustment tool and rotate the control clock-wise.PowerPre 500Mic PreampPhazeQ 500ModuleClose MicYou will find that most of the audible effect will be from 7 o’clock to 12 o’clock as this is where the bass frequencies are affected. As you move the control all the way clockwise (to 5 o’clock) the audible effect will diminish as it only affects the higher frequencies. This is because the human auditory system (our ears and brain) is most sensitive to phase shift at lower frequencies. Try depressing the 180º switch. This will reverse the polarity of the signal from 0º to 180º to effectively shift the phase between 181º to 360º. The effects can be dramatic, weird and very pleasing. There are no rules… just fun!True to the Music®Another great application is combining the direct feed from a guitar amp with a mic. One of the most diffi cult sounds to capture is an electric guitar. This is because the sound that the guitarist hears comes from a combination of the amp, the distance he is standing away from the source and the room itself. Take a direct feed from the amp using a Radial JDX Reactor. Feed this into the PhazeQ and set up a second mic in front of the amp or in the room. Combine, mix and phase adjust and you will be amazed at how quickly you can take a very basic sound and turn it into a monster. The same works on bass, kick drum, piano… once you try it you will never record without one. It is truly addictive!PowerPre 500 Mic PreampPhazeQ 500Using The PhazeQ As An EqualizerConnect a source or pre-recorded track to the PhazeQ and then send the output to a single channel on your mixer. Start by setting the phase adjustment counter-clock wise, 180º out, and filter off. Set the BLEND control counter- clockwise so that you will only hear the dry sound. Check to make sure you have signal. Turn on the phase, set the BLEND control to12 o’clock, and slowly rotate the phase adjustment tool. You will immediately hear the effect.A pleasing effect is to phase cancel frequencies in the mid range. Because we humans are more sensitive to mids (as this is where we communicate), cutting the mids can make our voices sound big and brash… or give the character of an FM Radio DJ. This can be effective on acoustic instruments, drums and all types of other instruments. The only way to really understand how this works is by trying it. The more you play with it, the more you will begin to realize the scope. It is huge.®True to the MusicUsing The Low-pass FilterFor even more fun, you can bring in the low-pass fi lter. This is basically a fi lter that removes high frequencies from the phase shift circuit. It is set with two ranges so that you will be able to apply a slight, yet well controlled roll off in the upper registers or apply brute force to the mids and low end. In either case, the intent is to focus the phase effect while leaving the highs alone. The controls let you decide where the effect will occur.When using the phase, set it fully clock-wise to 5 o’clock and then slowly dial it in backwards. As you move the control counter-clockwise, you will attenuate the high frequencies which means that the direct (un-phased) signal will no longer be competing with the out-of-phase signal. This can be particularly advantageous with acoustic instruments that are more phase sensitive in the upper registers. Once again there are no rules, only choices.Thank you for taking the time to read throught this manual. The suggested uses and applications outlined are simply an introduction to the capabilities of the PhazeQ 500 module. Enjoy!True to the Music®Radial Engineering Ltd.P HAZE Q 500 ModuleBLOCK DIAGRAM*PHASEQ 500 MODULE SPECIFICATIONS** Subject to change without notice.9CONNECTOR WIRINGGROUNDHOT (+)COLD (-)GROUNDTRS ¼” Phone ConnectorHOT (+)TS ¼” Phone ConnectorXLR ConnectorCircuit Type:Discrete Component Class A Frequency Response: 20Hz~20KHz Dynamic Range: 102dBNoise - Dry: -90dB below0dBu Noise - Wet: -80dB below 0dBu Maximum Output: +17dBuTHD+N - Dry: >0.002% @1kHz (0dB input, 100k load) THD+N - Wet:>0.015% @1kHz (0dB input, 100k load) Intermodulation Distortion: >0.015% @ -12dBu out Input Impedance: 3.4K Ohms Output Impedance: 110 OhmsPhase Shift (wet): Range 1: 12 to 180 degreesRange 2: (inverted) 192 to 360 degrees Low Pass Filter: Range 1: 300Hz ~ 3.8kHzRange 2: 3kHz ~ 38kHzOmniport:Dry Signal, Balanced Output, +15dBu Power Requirement: 50mAShipping Weight: 1.5 lbs. (0.7 kg)Warranty:3 Years, TransferableBYPASSOMNIPORT+XLRINPUT+XLROUTPUTBALANCED TO UNBALANCED CONVERTERPHASE SHIFT ADJUST LOW PASS FILTERBLEND WET/DRYCONTROLUNBALANCED TO BALANCED CONVERTERUNBALANCED TO BALANCED CONVERTERPHASE RANGEFILTER RANGE +-FILTER BYPASS+-+-BYPASS®True to the MusicTHREE YEAR TRANSFERABLE LIMITED WARRANTYRADIAL ENGINEERING LTD. (“Radial”) warrants this product to be free from defects in material and workmanship and will remedy any such defects free of charge according to the terms of this warranty. Radial will repair or replace (at its option) any defective component(s) of this product (excluding fi nish and wear and tear on components under normal use) for a period of three (3) years from the original date of purchase. In the event that a particular product is no longer available, Radial reserves the right to replace the product with a similar product of equal or greater value. In the unlikely event that adefectisuncovered,**************************************************************number (Return Authorization number) before the 3 year warranty period expires. The product must be returned prepaid in the original shipping container (or equivalent) to Radial or to an authorized Radial repair centre and you must assume the risk of loss or damage. A copy of the original invoice showing date of purchase and the dealer name must accompany any request for work to be performed under this limited and transferable warranty. This warranty shall not apply if the product has been damaged due to abuse, misuse, misapplication, accident or as a result of service or modifi cation by any other than an authorized Radial repair centre.THERE ARE NO EXPRESSED WARRANTIES OTHER THAN THOSE ON THE FACE HEREOF AND DESCRIBED ABOVE. NO WARRANTIES WHETHER EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO, ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL EXTEND BEYOND THE RESPECTIVE WARRANTY PERIOD DESCRIBED ABOVE OF THREE YEARS. RADIAL SHALL NOT BE RESPONSIBLE OR LIABLE FOR ANY SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES OR LOSS ARISING FROM THE USE OF THIS PRODUCT. THIS WARRANTY GIVES YOU SPECIFIC LEGAL RIGHTS, AND YOU MAY ALSO HAVE OTHER RIGHTS, WHICH MAY VARY DEPENDING ON WHERE YOU LIVE AND WHERE THE PRODUCT WAS PURCHASED.API, Lunchbox, Protools and Neve are registered trademarks of their respective owners.Reamp, Reamper, Reamping and Workhorse are trademarks of Radial Engineering Ltd.Copyright 2011 Radial Engineering Ltd. All rights reserved. Specifi cations and appearances subject to change without notice.Radial ® PhazeQ ™ 500 Module User Guide Rev1.0 April 2011 - Part #: R870 1248 00Printed in CanadaThis product is intended for professional use only. The user should be familiar and experienced with the 500 series rack and module formatRadial Engineering Ltd.1588 Kebet Way, Port Coquitlam, British Columbia, V3C 5M5 tel:604-942-1001•fax:604-942-1010•email:******************。
