Chromeleon色谱工作站 操作指南

第1篇一、引言色谱光谱仪器是一种用于分析样品中成分的精密仪器，广泛应用于化学、生物、医药、环保等领域。

为确保实验结果的准确性和仪器的安全运行，以下为色谱光谱仪器的操作规程。

二、操作前的准备1. 确保仪器电源正常，连接好所有管线。

2. 检查仪器各部件是否完好，如进样器、检测器、柱箱、汽化室等。

3. 根据实验要求，准备好所需的样品、溶剂、试剂等。

4. 熟悉仪器的操作面板，了解各按键、旋钮的功能。

三、开机与预热1. 打开仪器电源开关，预热30分钟至1小时，使仪器达到稳定状态。

2. 根据实验要求，设置柱温、汽化温度、检测温度等参数。

3. 打开载气（如氮气、氢气等）钢瓶总阀，调节减压阀开关，使输出压力在规定范围内。

4. 调节仪器上的载气调压阀，使柱前压达到分析工作所需压力。

5. 打开空气发生器电源，扭开氢气钢瓶阀门，调节氢气减压阀压力。

6. 调节仪器正面右下侧的针形阀，使空气压力和氢气压力符合实验要求。

7. 用点火枪点着FID的火焰，用玻璃片或铁片等冷的物体靠近检测器的盖帽，有水珠凝结表明点火成功。

四、样品分析1. 将样品溶液注入进样器，根据实验要求设置进样量。

2. 启动仪器，待基线稳定后开始分析测试工作。

3. 观察色谱图，分析样品中各成分的保留时间、峰面积等信息。

4. 根据实验要求，调整色谱条件，优化分离效果。

五、关机与维护1. 分析工作结束后，关闭氢气钢瓶总阀和空气发生器电源。

2. 调低各路设定温度，使柱温箱、汽化室、检测器温度下降，待柱箱温度低于70℃时关闭仪器电源。

3. 关闭载气钢瓶总阀。

4. 清理仪器室的进样针、样品等物品。

5. 定期对仪器进行维护保养，如清洗进样器、更换滤头、检查管线等。

六、注意事项1. 操作过程中，严格遵守安全规程，防止发生意外事故。

2. 仪器操作人员应具备一定的化学分析基础，熟悉仪器操作。

3. 注意仪器的温度、压力等参数，确保实验结果准确。

4. 实验结束后，及时整理实验数据，进行结果分析。

U-3000液相色谱使用手册之三Chromeleon 中文版U-3000样品表的建立2012.TMO戴安产品培训中心.★服务热线：800-810-5118, 400-650-5118★客服邮箱：★市场培训：0(电话) 0 (传真)(邮箱)目录说明 (3)一．色谱仪器控制区(Console) (4)二．建立一个仪器方法 (5)1．采集时间和采集通道设置 (5)2．LPG-3400SD泵常规设置 (6)3．泵流速梯度的设定 (6)4．WPS-3000自动进样器的常规设置 (7)5．进样模式 (8)6．TCC-3100柱温箱常规设置 (10)7．UV(DAD-3000RS)紫外二极管阵列检测器通道设定 (12)8．新建立仪器方法的预览与修改 (13)9．保存新建并修改过的仪器方法 (15)三．创建一个处理方法 (16) (18)202021 4．输入待测样品个数225. 输入每个待测样品进样次数 (22)6．设置自动进样器开始位置 (22)7．输入待测样品进样体积 (22)8．建好待测样品表 (23)9．为新建序列指定所用方法和报告 (23)10．保存新建序列表 (23)六、修改样品序列表 (25)1. 修改样品名称 (25)2．修改样品类型 (25)3．修改样品瓶位置 (27)4．修改进样体积 (27)5．选择所用仪器方法 (27)6．选择所用处理方法 (28)7．状态 (28)8．样品重量 (28)9．稀释因子 (28)10．内标量 (28)11．保存经修改后的样品序列表 (28)说明利用液相色谱来分析一批样品，一般需要五个文件来完成。

1、需要一个控制面板（e panel）控制面板是实验人员通过计算机与仪器交流的直接界面。

而且在通讯连接正常时，仪器的所有参数都在面板上反应并被记录。

2、需要一个仪器方法(Instrument Method)规定测定这些样品时所用到的系统的条件：比如泵的流速，压力上下限；自动进样器/手动进样阀的进样持续时间；检测器波长；柱温箱的温度；测每个样品需要的时间等等。

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Chromeleon色谱工作站操作指南戴安中国有限公司技术服务中心2003.2注意：以下操作步骤以ICS-1500，手动进样为例。

“”为单击鼠标左键；“”为双击鼠标左键；“”为单击鼠标右键。

* 建立控制面板1. 从“开始”处依次选择Programs, Chromeleon和Chromeleon；2. 点击计算机代码，双击与实际情况一致的控制面板；3. 确认各模块的“Connected”图标均为绿色后，设置淋洗液的液位开泵，设定流速；5. 点击兰色圆形图标（Acquisition On/Off）；6. 进入Pump_ECD – ECD_1；7. 设置电导池和柱温箱的温度，选择抑制器类型， 输入淋洗液浓度，点击“确定”开始采集基线。

* 设定运行程序1. 依次点击File和New，选择Program File；2. 点击“下一步”；3. 设置高/低压极限和流速，点击“下一步”；4. 设置手动进样，进样时间为60秒；5. 将Acquisition t ime调整至0～15分钟，点击“下一步”；6. 设置电导池和柱温箱的温度，选择抑制器类型， 输入淋洗液浓度，点击“下一步”；7. 点击“下一步”；8. 选择“Review the program in a new window”，点击“完成”；注意：绿色字体为注释信息，不起作用。

10. 存盘退出（推荐存入独立的“program”子目录中）。

* 建立方法文件1.依次点击File和New，选择Method File；2. 依次点击File和Save as，存盘退出（推荐存入独立的“method”子目录中）。

* 建立样品表1.依次点击File和New，选择Sequence(using Wizard)；2.点击“下一步”（连续四次）；3. 选择正确的程序文件，点击“Open”；4.选择正确的方法文件，点击“Open”；5. 选择正确的报告文件，点击“Open”；6. 存盘退出；5.修改标准点的名称、类型和数量。

Chromeleon色谱工作站操作指南戴安中国有限公司技术服务中心2005.2注意：以下操作步骤以阴离子分析，手动进样为例。

“”为单击鼠标左键；“”为双击鼠标左键；“”为单击鼠标右键。

* 建立控制面板1. 从“开始”处依次选择Programs, Chromeleon和Chromeleon；2. 点击计算机代码，双击与实际情况一致的控制面板；3. 确认各模块的“Connected”图标均为绿色后，设置淋洗液的液位开泵，设定流速、淋洗液浓度；注意：如果操作条件固定（不进行排气泡、更换系统等操作），开/关机分别点击Startup和Shutdown即可。

5. 点击兰色圆形图标（Acquisition On/Off）；6. 进入Pump_ECD – ECD_1；7. 设置电导池和柱温箱的温度，选择抑制器类型， 输入淋洗液浓度，点击“确定”开始采集基线。

* 设定运行程序1. 依次点击File和New，选择Program File；2. 点击“下一步”；3. 设置低/高压极限（200~3000psi）和流速，点击“下一步”；4-1.等浓度淋洗时,直接在“Start”处输入浓度，点击“下一步”；4-2. 梯度淋洗时,选择“Multi-Step Gradient”，点击“下一步”；4-3. 根据分析方法，在“Concentration”处输入起始浓度，点击键盘的“↓”，选择“是（Y）”；。

注意：Curve下必须键入1-9之间的数值（推荐值：5）。

5. 设置手动进样，进样时间为60秒；6. 将Acquisition t ime调整至0～15分钟，点击“下一步”；7. 设置电导池和柱温箱的温度，选择抑制器类型， 输入淋洗液浓度，点击“下一步”；8. 点击“下一步”；9. 选择“Review the program in a new window”，点击“完成”；10. 将引号中的内容修改为中文（例如：请进样）；注意：绿色字体为注释信息，不起作用。

色谱工作站的操作方法一、操作步骤；1、色谱工作站在通用版色谱工作站的基础上，以GB50325-2010及GB/T18883-2002为准则，为方便计算增加的功能：自动套用甲苯的工作曲线计算谱图中所有未知峰的总含量。

可计算单位采样体积中各组份总的含量，并可换算到标准状态下。

可扣除溶剂中各组份的峰面积和室外空气样品中各组份的含量。

2、谱图处理：这步的主要任务是正确地将谱图听峰检测到，并为这些峰确立恰当的基线，以及为重叠的峰确立恰当的分割线。

程序在采集谱图的过程中会对谱图进行实时处理，但也在谱图采集结束后，调节“谱图处理能数”或制定“谱图处理方案”后对谱图进行再处理。

3、定量计算：在第一步中得到了谱图中各个峰的位置和大小就可以在这些信息的基础上有一系列已知浓度的标样计算各气体组份的工作曲线，或反过来有工作曲线计算待测样品中各气体组份的浓度。

4、谱图采集与处理：①在“工具”菜单里执行“选项”命令，在弹出的选项对话框的“操作”页面设置“通道貌岸然同步”一项。

②在“文件“菜单内选择“新建”命令，程序将打开一新窗口，并同时试图寻找一个名为“默认模板”的文件，如果找到这个文件则将其中记载的几张表格内容（如“谱图参数表”、“谱图处理表”、“定量组份表”、“定量方法表”、“分析报告表”）引进到新建窗口的相应相应表格中来，如果没找到程序弹出一对话框没有，故新建窗口中表选择“新建”命令，程序将打开一新窗口，并同时试图寻找一个名为“默认模板”的文件，如果找到这个文件则将其中记载的几张表格内容（如“谱图参数表”、“谱图处理表”、“定量组份表”、“定量方法表”、“分析报告表”）引进到新建窗口的相应表格中来，如果没找到程序弹出一对话框没有，故新建窗口中表格的内容采胜出厂值。

③在“谱图参数表”“信号通道里选择谱图信号的采集通道。

④选择“操作”菜单中的“谱图采集”命令，这时谱图窗口内开始有谱图走动。

⑤如果要调节谱图在横向和纵向上的缩放，请分别调节“谱图参数表”中“满屏时间”和“满屏量程”两个谱图显示参数，也可分别单击这两个参数旁的“满屏”按钮，使当前已采集到的谱图分别在横向和纵向上满屏。

Chromeleon色谱工作站操作指南2024版在化学分析领域，Chromeleon 色谱工作站是一款功能强大且广泛应用的工具。

它能够帮助我们高效地处理和分析色谱数据，为实验研究和质量控制提供有力支持。

接下来，让我们详细了解一下 Chromeleon色谱工作站 2024 版的操作流程。

一、软件安装与启动首先，确保您已经获得了合法的 Chromeleon 色谱工作站 2024 版安装程序。

插入安装光盘或从官方网站下载安装文件，按照安装向导的提示逐步进行操作。

在安装过程中，您需要选择安装路径、语言等选项。

安装完成后，在桌面上会出现Chromeleon 色谱工作站的快捷方式。

双击该快捷方式，启动软件。

启动后，您会看到软件的主界面，包括菜单栏、工具栏、状态栏等。

二、仪器连接与设置在使用 Chromeleon 色谱工作站之前，需要将色谱仪器与计算机正确连接。

通常，可以通过USB、以太网等接口进行连接。

连接完成后，在软件中进行仪器设置。

点击菜单栏中的“仪器”选项，选择“配置仪器”。

在弹出的对话框中，选择您所使用的色谱仪器型号，并设置相应的通信参数，如端口号、波特率等。

设置完成后，点击“测试连接”按钮，确保软件能够与仪器正常通信。

三、方法创建方法是色谱分析的重要参数，包括色谱柱、流动相、流速、检测波长等。

在 Chromeleon 色谱工作站中，可以方便地创建新的分析方法。

点击菜单栏中的“方法”选项，选择“新建方法”。

在方法编辑界面中，依次设置各个参数。

例如，在“色谱柱”选项中，选择您所使用的色谱柱型号和规格；在“流动相”选项中，设置流动相的组成和比例；在“流速”选项中，输入合适的流速值；在“检测波长”选项中，选择您需要检测的波长。

设置完成后，点击“保存”按钮，将方法保存起来，以便后续使用。

四、样品信息录入在进行色谱分析之前，需要录入样品的相关信息，如样品名称、编号、来源等。

点击菜单栏中的“样品”选项，选择“新建样品”。

Chromeleon色谱工作站操作指南戴安中国有限公司技术服务中心2005.2注意：以下操作步骤以阴离子分析，手动进样为例。

“”为单击鼠标左键；“”为双击鼠标左键；“”为单击鼠标右键。

* 建立控制面板1. 从“开始”处依次选择Programs, Chromeleon和Chromeleon；2. 点击计算机代码，双击与实际情况一致的控制面板；3. 确认各模块的“Connected”图标均为绿色后，设置淋洗液的液位开泵，设定流速、淋洗液浓度；注意：如果操作条件固定（不进行排气泡、更换系统等操作），开/关机分别点击Startup和Shutdown即可。

5. 点击兰色圆形图标（Acquisition On/Off）；6. 进入Pump_ECD – ECD_1；7. 设置电导池和柱温箱的温度，选择抑制器类型， 输入淋洗液浓度，点击“确定”开始采集基线。

* 设定运行程序1. 依次点击File和New，选择Program File；2. 点击“下一步”；3. 设置低/高压极限（200~3000psi）和流速，点击“下一步”；4-1.等浓度淋洗时,直接在“Start”处输入浓度，点击“下一步”；4-2. 梯度淋洗时,选择“Multi-Step Gradient”，点击“下一步”；4-3. 根据分析方法，在“Concentration”处输入起始浓度，点击键盘的“↓”，选择“是（Y）”；。

注意：Curve下必须键入1-9之间的数值（推荐值：5）。

5. 设置手动进样，进样时间为60秒；6. 将Acquisition t ime调整至0～15分钟，点击“下一步”；7. 设置电导池和柱温箱的温度，选择抑制器类型， 输入淋洗液浓度，点击“下一步”；8. 点击“下一步”；9. 选择“Review the program in a new window”，点击“完成”；10. 将引号中的内容修改为中文（例如：请进样）；注意：绿色字体为注释信息，不起作用。