LY2183240_874902-19-9_DataSheet_MedChemExpress

SHP2和PDL1在胰腺导管腺癌中的表达及临床意义郑佳薇1,黄于芳2,宋丽3,欧阳学农3(1.莆田学院基础医学部，福建莆田351100；2.福建省肿瘤医院肿瘤内科，福建福州350014；3.联勤保障部队第900医院肿瘤科，福建福州350025)摘要：目的探讨Src同源区2-含蛋白酪氨酸磷酸酶2(SHP2)和程序性细胞死亡1配体1(PDL1)在胰腺导管腺癌中 的表达及临床意义。

方法选取2008年1月至2017年12月间联勤保障部队第900医院(原福州总医院)病理科胰腺导管腺癌(Pancreatic ductal adenocarcinoma,PDAC)患者手术切除的79例存档蜡块标本，使用免疫组织化学法检测其石蜡包埋标本中SHP2和PDL1的表达情况。

结果79例PDAC患者中，SHP2表达、PDL1表达、SHP2及PDL1双阳性表达分别为49例(62.03%)、62例(78.48%)和47例(59.49%)。

SHP2表达与PDL1表达之间存在显著的相关性(:=0.542,P=0.000)；SHP2表达与性别、年龄、分化差显著相关(P<0.05)；PDL1表达与性别相关(P<0.05)；SHP2/PDL1双阳性表达与性别、年龄、分化差、手术切缘显著相关(P<0.05)。

结论SHP2和PDL1在PDAC患者中的高表达与其发生发展具有潜在的相关性，有可能成为治疗该疾病的靶点。

关键词：SHP2；PDL1；胰腺导管腺癌中图分类号：R735.9文献标识码：A文章编号:1001-7550(2021)01-0055-04The Expression and Clinical Significance of SHP2and PDL1in Pancreatic Ductal Adenocarcinoma ZHENG Jia-wei et al(School of Basic Medical Science,Putian University,Putian351100,China)Abstract:Objective To explore the expression and clinical significance of Src homologous region2-containing protein tyrosine phosphatase2(SHP2)and programmed cell death1ligand1(PDL1)in pancreatic ductal adenocarcinoma(PDAC) .Methods From January2008to December2017,79archived wax specimens of PDAC surgically removed from the900th Hospital of the Joint Logistics Support Force(formerly Fuzhou General Hospital)Histochemical method was used to detect the expression of SHP2and PDL1in par­affin-embedded specimens.Results Among79PDAC patients,SHP2expression,PDL1expression and SHP2/PDL1co-expression were detected in49cases(62.03%),62cases(78.48%)and47cases(59.49%)patients,respectively^.A significant association be­tween SHP2expression and PDL1expression was observed(r$=0.542,P=0.000).SHP2expression was significantly associated with gender,age,poor differentiation(P<0.05).PDL1expression was associated with gender(P<0.05).SHP2/PDLl co-expression was sig­nificantly associated with gender,age,poor differentiation and surgical margin(P<0.05).Conclusion High SHP2/PDL1expression has a potential correlation with tumor genesis and development in PDAC patients and may be a potential target for therapy in PDAC.Key words:SHP2；PDL1；pancreatic ductal adenocarcinoma胰腺导管腺癌(Pancreatic ductal adenocarcino-ma,PDAC)由于早期症状不明显、易转移和对放疗、化疗等治疗方式的敏感性不高(唯一的根治性方法为手术切除)导致诊断时多发展为晚期，同时，如果患者术后没有配合有效的辅助治疗均导致患者术后5年生存率低于5%［1］。

Colorimetric Aldehyde Assay Kit Catalog Number MAK139Storage Temperature –20 °CTECHNICAL BULLETINProduct DescriptionAldehydes are highly reactive molecules, which play a role in many physiological, pathological and commercial processes. They have been implicated asenvironmental pollutants, and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool forbiological and chemical research, the food industry, and environmental pollution surveillance.The Colorimetric Aldehyde Assay Kit provides a simple and direct procedure for measuring aldehydes in a variety of samples. Aldehydes reacts with a dye, resulting in a colorimetric (405 or 550 nm) product proportional to the amount of aldehyde present. The Colorimetric Aldehyde Assay Kit has a lower limit of detection of 1 nanomole of aldehyde in a 100 µL assay volume. This kit has been used for monitoring activities of oxidases that convert an amino group to an aldehyde group.ComponentsThe kit is sufficient for 200 assays in 96 well plates.Aldehyde Detection Reagent2 ea Catalog Number MAK139A Assay Solution10 mL Catalog Number MAK139B Aldehyde Standard1 vl Catalog Number MAK139C Dilution Buffer20 mLCatalog Number MAK139DReagents and Equipment Required but Not Provided.• 96 well flat-bottom plate –It is recommended to useclear plates or white plates with clear bottoms for colorimetric assays.• Spectrophotometric multiwell plate reader Precautions and DisclaimerThis product is for R&D use only, not for drug,household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.Preparation InstructionsBriefly centrifuge vials before opening. Use ultrapure water for the preparation of reagents. To maintain reagent integrity, avoid repeated freeze/thaw cycles.Aldehyde Detection Reagent, Assay Solution, DilutionBuffer –Allow them to come to room temperature before use.Aldehyde Standard –Reconstitute with 1 mL of DilutionBuffer to generate a 10 mM Aldehyde Standard stock solution. Mix well by pipetting, then aliquot and store at –20 °C. Storage/StabilityThe kit is shipped under ambient conditions and storage at –20 °C, protected from light, is recommended.ProcedureAldehyde Standards for Colorimetric Detection Dilute 100 µl of the 10 mM Standard with 900 µl of Assay Buffer to prepare a 1,000 µM standard solution. Further dilute the 1,000 µM standard solution by 10-fold and 3-fold serial dilutions with Dilution Buffer. Add 50 µL of the diluted standard solutions into a 96 well plate, generating 0 (blank), 1, 3.3,10, 33, 100, 333, and 1,000 µM standards.2Sample PreparationAdd 0–50 µL of samples into wells. For unknown samples, it is suggested to test several sample dilutions to ensure the readings are within the linear range of the standard curve. If analyzing enzyme reactions, for example, fructose-1,6-biphosphate aldolase and fructose-1,6-biphosphate, prepare a 50 µL of enzyme reaction and incubate the enzyme reaction at 37 °C for at least one hour.Note: Samples from enzyme reactions may need to be optimized in regards to the components of the reaction (for example, buffer solution). Both BSA (>0.001%) and TWEEN®20 (>0.01%) will interfere with the assay and should be avoided during sample preparation. For most reactions, the Dilution Buffer can also be used for running enzyme reactions.Assay Reaction for One 96 well Plate1.Set up the Master Reaction Mix by adding 5 mL ofAssay Solution to 1 bottle of Aldehyde DetectionReagent. 50 µL of the Master Reaction Mix isrequired for each reaction (well).Note:The Master Reaction Mix is enough for oneplate. The Master Reaction Mix is not stable andbest used within 2 hours.2.Add 50 µL of the Master Reaction Mix to each ofthe standard, blank control, and sample wells. Mix well using a horizontal shaker or by pipetting, andincubate the reaction for 30–60 minutes at roomtemperature. Protect the plate from light during the incubation.3.Measure the absorbance at either 405 or 550 nm.Note: Samples with lower aldehyde concentrations should be read at 405 nm while samples withhigher concentrations should be read at 550 nm.ResultsCalculationsThe background for the assay is the value obtained for the 0 (blank) Aldehyde standard. Correct for the background by subtracting the blank value from all readings. Background values can be significant and must be subtracted from all readings.Use the values obtained from the appropriate Aldehyde standards to plot a standard curve.Note: A new standard curve must be set up each time the assay is run.Using the corrected measurement, the concentration of aldehyde present in the samples may be determined from the standard curve.3TWEEN is a registered trademark of Croda International PLC.LS,MAM 03/14-1©2014 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at and/or on the reverse side of the invoice or packing slip.。

Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:Oct.-01-2018Print Date:Oct.-01-20181. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :ITI214Catalog No. :HY-12501ACAS No. :1642303-38-51.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:NoneFormula:C29H29FN7O5PMolecular Weight:605.56CAS No. :1642303-38-54. 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