n Some exceptions
u In certain diseases, a single titer of sufficient magnitude can be used as
n Stained microscopic observations
u Gram stain u Acid-fast stain u Fluorescence stain
10
11
12
13
14
15
16
Isolation & Culture: Colony
n Size n Shape n Color n Surface features
u Positive if the titer of the later specimen is above 4X to the first specimen
n Transport, and store correctly
u as soon as possible u Store at -4oC for most bacterial specimens, but keep warm for
SerologicaБайду номын сангаас assays
antibiotics susceptibility
8
Diagnosis Strategy for Bacterial Infection
9
Morphological
n Non-stained microscopic observation
u Dark-field microscopy u Observing the movement of live bacteria
n Major drawbacks
