2. Experimental section
• Chemicals and reagents.
• Scutellarin,Scutellarein,4-MU,Tris-HCl, • 7-hydroxycoumarin,UDPGA(缓冲盐)， • 重组UGTs亚型〔UGT1A1，UGT1A6， • UGT1A9，UGT2B7〕，HPLC reagents.
• UGTs have been demonstrated to be involved in the
metabolic elimination of many important endogenous substances, such as bilirubin, bile acid and estradiol (Erichsen et al., 2021).
• Incubation and analysis methods for inhibition
aluation.
After 5min pre-incubation at 37℃, the UDPGA was added in the mixture to initiate the reaction. The incubation time was 120min for UGT1A1 and UGT2B7, 30min,or UGT1A6 and UGT1A9, respectively. The reactions were terminated by adding 100 mL acetonitrile with 7-hydroxycoumarin (100mM) as internal standard.The mixture was centrifuged at 20000×g for 10min, HPLC analysis.
