MCE-Anti-c-Myc-Magnetic-Beads-Manual-cn

1包装清单产品概述MCE 磁力架是磁珠产品专用配套设备，支持 MCE 全线磁珠类产品，内含强磁磁芯，可实现快速高效的分离。

MCE 磁力架 (200 μL / 2 mL / 15 mL ) 采用独特的三明治槽设计，磁条可抽出，可容纳 200 μL PCR 管，1.5 mL EP 管，2 mL EP 管，15 mL 离心管。

本产品适用于抗体纯化、免疫沉淀 (IP )、免疫共沉淀 (Co-IP )、细胞分选和核酸分离等实验。

2操作说明31. 将装有磁珠悬液的 EP 管/离心管置于磁力架对应的样品孔中，静置数分钟后磁珠被吸附聚集于管壁，溶液恢复澄清。

2. 用移液器或吸管从管底将溶液吸出，或小心倾倒出液体。

3. 抽去磁力条，加入复溶液体，轻缓震荡即可混合均匀，进行下一步操作。

注：磁性分离的时间与磁珠粒径有关，磁珠粒径越小，磁性分离时间越长。

此外，溶液的黏稠程度以及溶液的成分也会对磁性分离时间产生影响。

5注意事项1. 根据实验参数和样品体积不同，可调整磁芯位置或选用不同样式的磁力架。

2. 为减少操作过程中磁珠的损失，请将样品管底端插入磁力架底部的凹槽内。

当磁珠吸附在管壁上后，缓慢倾去上清，或用移液枪吸尽。

3. 由于磁力架有强大的磁场，请远离手机、电脑、手表、起博器、磁铁等易被磁力干扰的物体，尤其是刀具，以免对操作人员造成伤害。

4. 如需同时使用多个磁力架 (≥2 个) ，应分开放置，避免磁场之间产生干扰。

不要把多个磁棒放在一起，以防止夹伤。

5. 请勿与强酸、强碱等腐蚀性溶剂直接接触。

6. 请勿拆卸磁块。

7. 为保护外壳，请勿长时间暴露在阳光和紫外线下。

8. 为保持磁力架磁性，请勿置于高温和强外界磁场环境中。

9. 使用后请及时清洁，妥善放置在干燥环境中。

Magnetic StandMedChemExpress MedChemExpress 400-820-3792 电话: ************ 传真: ************Email: t *********************MCE Hotline: 400-820-3792Contents HY-K0200Magnetic Stand 200 μL-2 mL-15 mL。

Hirsch Teil1. What are chemical sensors?- Definition !!!2. Selectivity- Definition- Equilibrium based selectivity: free energy, dielectric constant and distance,- Kinetic based selectivity: steady-state regime3. Recognition Methods- Ion recognition: recognition-electric charge, selectivity-size,transduction-potentiometric, optical methodse.g. PH electrode ----> part 3- Recognition by affinity interactions: reversible, non-covalent bonds-ionic bonds, hydrogen bonds, van der Waals interaction => result in a molecular assiciation complex; also respect to shape and chemical reactivity; indicated by stability constant (very stable)- antibody - antigen interaction => immunochemical reactionantibody: glycoprotein produced by immune system to identify and neutralizepathogen microorganisms.antigen: the part of the pathogen that reactions with the antibody.use specific antibody receptor => identify pathogenuse antigen receptor => identify antibody (the detection of infection byparticular pathogen)- lectin proteins recognize caborhydrates (agglutinins, hemagglutinin)carbohydrate-binding modules link to the catalytic part of glycosidehydrolases => result in degradation of cell wall, storage of polysaccharide- A Molecularly Imprinted Polymer (MIP) is a polymer that has been processed usingthe molecular imprinting technique which leaves cavities in polymer matrix withaffinity to a chosen "template" molecule.In chemistry, molecular imprinting is a technique to create template-shaped cavities in polymer matrices with memory of the template molecules to be used in molecular recognition.-Nucleic acid aptamers are nucleic acid species that have been engineered throughrepeated rounds of in vitro selection to bind to various molecular targets such assmall molecules, proteins, nucleic acids, and even cells, tissues and organisms.Aptamers are useful in biotechnological and therapeutic a pplications as they offer molecular recognition properties that rival that of the commonly used bimolecular antibodies.- Recognition by nucleic acids: hydrogen bonds between two distinct pairs of nucleobases => two complementary nucleic acids form a double strand association complex => called hybridizationnucleic acid sensors: short single strand NA as receptor to recognize a particular NA sequence in the analyte NA => detection of genetic anomalies and pathogen mircoorganism- Recognition by enzyme: dynamic processEnzyme: protein compound that function as catalysts in chemical reaction occurring in living system.- Recognition by cells and tissues: advantages of enzyme incorporated in biological materials => in their natural environmentsee part 3, Wegener - Recognition by gases and vapors: based on sorption at solid material => surface-adsorption, inner-absorption; purely physical phenomenon or chemical reaction.4. Transduction MetohdosChemical transduction: monitoring the change of chemical composition of the sensing element in response to the recognition process. => change in concentration/amount is measured => detect primary product -> secondary product or coreagent -> labeling productLABEL can be a simple molecular species or nanoparticals that can be detected by available physiochemical methods => enzyme, fluorescent dyes, luminescent dyes, electroactive compoundsPhysical transduction: a specific physical property of the sensing element that is affected by its interaction with the analyte is monitored. => mass, reflective index, dielectric properties, electrical resistivity => LABEL-FREE- Thermometric transductionRecognition of the analyte leads to change in temperature => only catalytical processes generate sufficient heat to the measurement => application: combustible gases react with O2 at the surface of a catalyst.- Transduction based on mechanical effectsRecognition leads to change in mass of the sensing element => monitored by mass tranducer based on quartz crystal microbalance (QCM)----------------------------------------------------------------------------------------------------------------- QCM, correct name: Thickness shear modePiezoelectric effect:generation of electrical charges on the surface of a solid by strain, pressure or torsion (mechanical deformation of solid) =>electricity resulting from pressureI nverse piezoelectric effect:application of charges to surfaces of piezoelectricsolid generates mechanical deformation (elongation, contraction, torsion)QCM is based on Inverse piezoelectric effect!# AT cut => 35`15`=> minimum temperature coefficient at 50~70 CIt makes the AT-cut well suited to applications requiring high degree of frequency stability over wide temperature ranges.## Electrodes are applied on both sides, and AC voltage applied.DC cannot flow across the crystal because it consists of an insulator material;however the crystal somewhat behaves as capacitor and allow an AC current to f low along the left-hand loop.AC voltage applied => leads to shear oscillation of crystal => when the voltage frequency matches the intrinsic vibration frequency of the crystal => the vibration amplitude is at maximal => the resonant => resonant frequency (f0) => depend on crystal thickness (e.g. d q= 330 um, f0= 5MHZ), density and elasticity of piezoelectric material### AT-cut resonator: thickness: ~0.2 mm, diameter of the active area: 5~20 mm #### Deposition of a homogenous mass film (a rigid overlay)Sauerbrey equation:Cf indicate sensitivity of QCMcondition of this equation: rigid deposited mass; △m<2% of crystal mass;operated in vacuum or in gaseous atmopphereIn liquid: the liquid breaks the vibration by friction => lessen f0Thickness of the layer must be greater than the wave decay lengththat is of 250 nm of 5 MHz resonator at water. ----> part 2!!!##### QCM in practice => see p.41----------------------------------------------------------------------------------------------------------------- - Resistive and capacitive transductionRecognition leads to changes in the electrical property of this materialResistive transduction: gases interact with MOS => change in electrical resistivity Capactive transduction => dielectric constant- Electrochemical transductionsee part 2, Matysik - Optical transductionOptical transduction can be based on light emission or light absorption, also by physical quantity (reflective index) and light scattering.5. Sensor Configuration and Fabrication- Lateral flow assayA typical test strip consists of the following components:1. Sample pad – an absorbent pad onto which the test sample is applied2. Conjugate pad –this contains antibodies specific to the target analyte;conjugated to coloured particles (e.g. gold nanoparticles)3. Reaction membrane –typically a hydrophobic nitrocellulose or celluloseacetate membrane onto which anti-target analyte antibodies are immobilized in a line across the membrane as a capture zone or test line, and a control zonecontaining antibodies specific for the conjugate antibodies.4. Wicking pad –a further absorbent pad designed to draw the sample acrossthe reaction membrane by capillary action and collect it.Double antibody sandwich assays: the sample migrates from the sample pad through the conjugate pad where any target analyte present will bind to the c onjugate.=> The sample then continues to migrate across the membrane until it reaches the test line where the target or conjugate complex will bind to the immobilized antibodies producing a visible line on the membrane. => The sample then migrates further along the strip until it reaches the control line, where excess conjugate will bind and producea second visible line on the membrane.This control line indicates that the sample has migrated across the membrane as intended. Two clear lines on the membrane is a positive result. A single line in the control zone is a negative result. Double antibody sandwich assays are most suitable for larger analytes, such as bacterial pathogens and viruses, with multiple antigenic sites. 6. Methods and Material in Sensor Preparation- Immobilization at solid surface => integration of a transducer with the receptor Physical adsorption at a solid supportNon covalent immobilization at solid surface => hydrophobic interaction, hydrogen bonding, electrostatic attraction; monolayer; no restrict access; not stable; Langmuir isotherm -> equilibrium interactionSupport material: silica, cellulose acetate, PVCCovalent bonding to the solid supportCovalent conjugation => stable, covalent bond, time consuming, expensiveCommon reactive group: -OH, -NH2, -C=O, -SH- Carboxylic acid with DCC- Glutaraldehyde reacts with the a.a. of lysine in protein => widely used Support: porous material => high specific area, high density of immobilized compounds => hydrogel: immobilized by entrapment/covalent corsslink - Natural polymers: Cellulose, Dextran- Synthetic polymers: Polystyrene- Active polymers: Epoxide (without preliminary activation) -->DNA array !!!- Inactive Polymers: Vicinal hydroxyls actived by CNBr- Inorganic support: Silica, AL2O3, TiO2 => stable at extreme PH- Metal support: noble metals, thiols on golds --> self assembled monolayers!Affinity reaction: avidin-biotin !!!Thin molecular layers: one or several molecular layers in solid support - Self-assembly of amphiphilic compounds: preparation of liposome andmicelles; liposome can be used of entrapment of molecular- Bilipid layer membranes: Langmuir-Blodgett technique- Layer by Layer assembly- Sol-Gel chemistry methods: silica gel => -O-Si-O-- Hydrogels: Xerogel, aerogel- Conducting polymers: Polyacetylene, polyaniline --> gas senor based on CP (----> part 3 !!!); also as entrapment matrix for biological receptors- Mesoporous materials: porous materials with pore (diameter: 2-50 nm,close to protein) => enzyme immobilization by entrapment (crosslinking withglutaraldehyde)- Deposition of polymers onto solid surfaces: dip coating, drop coating, spin coating ----> part 2 !!!Perm-selective memberanes: Nafin ----> Clark oxygen electrode Support-free crosslinkingEntrapment in a polymer networkEncapsulation7. Microfabrication Methodes- Spot Arraying: Contact-based & Noncontact-based; DNA microarray !!!!!Pros & Cons- Thick-film Technology: screen-printing technique (5-50 um thick layer)- Thin-film Technology: Photolithography (2 um)- Softlithography ----> experiment !!!!- Microcontact printing ----> experiment !!!!8. Optical Sensors- Electromagnetic RadiationOptical sensor => interaction of electromagnetic radiation with sensor layer - frequency; wavelength; photon energy (definition)- Structure: integration with wavelength-selection (optical filters) device and light sources (lasers), light detectors (phototransistors)- Optical Waveguides- Optical FibersOptical fibers' structuretotal internal reflection => evanescent wave- Spectrochemical Transduction MethodsSpectrochemical method analysis => light absorption or emission by sample => optical label performs absorption or emission (organic dye or metal complexes) - Light absorption: absorbance => concentration; sensitivity => thickness, absorpyivity, absorptivity => wavelength- Diffuse reflectance spectrometry: refelctance => concentration; suitable forsolid in near IR- Luminescence: Fluorescence spectromerty => fluorophore (label, organic dye or metal complexes, luminescent nanparticle ); steady-statefluorescence measurement, Time-resolved fluormetry; fluorescencequenching; resonance energy transfer (FRET); chemical- andbioluminescence => luminol; electrochemicaluminescence; Ramanspetrometry- Surface Plasmon Resonance Spectroscopy (SPR)。

细胞自噬染色检测试剂盒(MDC 法)产品简介：碧云天生产的细胞自噬染色检测试剂盒(MDC 法)，即Autophagy Staining Assay Kit with MDC ，是一种使用丹酰尸胺，也称单丹磺酰尸胺、丹酰尸胺或丹酰戊二胺(monodansylcadaverine, MDC)作为荧光探针快速便捷地检测细胞自噬的试剂盒。

自噬(autophagy)是一种在进化上高度保守的通过溶酶体吞噬并降解部分自身组分的细胞内分解代谢途径。

自噬与多种生理功能有关，在饥饿等环境条件下，细胞通过自噬降解多余或异常的细胞内组分，为细胞的生存提供能量及原材料，促进生物体的生长发育、细胞分化及对环境变化产生应答。

自噬异常与多种病理过程如肿瘤、神经退行性疾病、代谢疾病、病原体感染等都有密切关系。

由于细胞自噬在生理和病理过程中都有重要作用，自噬已经成为细胞生物学领域的一个研究热点。

MDC 是细胞自噬检测最常用的荧光探针之一。

MDC 可以通过离子捕获(ion trapping)和与膜脂的特异性结合，从而特异性标记自噬体(autophagosome)，也称autophagic vacuole ，因而常用于细胞自噬的检测。

MDC 是一种嗜酸性荧光探针，很多酸性膜性结构也会被MDC 染色，因此MDC 染色时正常的细胞也会有一定的染色背景。

本产品的染色原理决定了本产品只能用于培养的细胞或者组织的细胞自噬荧光染色检测，不能用于冻存的或固定的细胞、组织或者组织切片的染色检测。

使用本产品染色后可以通过荧光显微镜拍照观察，也可以通过荧光酶标仪或流式细胞仪进行荧光检测。

荧光显微镜观察时可以使用紫外区激发光激发，发出绿色荧光。

荧光酶标仪或流式细胞仪推荐的激发波长为335nm (330-360nm 均可)，发射波长为512nm (510-540nm 均可)。

本产品用于细胞自噬染色的效果参考图1。

图1. 细胞自噬染色检测试剂盒(MDC 法)的染色效果图。

碧云天生物技术/Beyotime Biotechnology订货热线：400-168-3301或800-8283301订货e-mail：******************技术咨询：*****************网址：碧云天网站微信公众号生物素标记EMSA探针－β-Catenin/TCF (0.2μM)产品编号产品名称包装GS018B 生物素标记EMSA探针－β-Catenin/TCF (0.2µM) 200µl产品简介：生物素标记EMSA探针－β-Catenin/TCF是用于EMSA(也称gel shift)研究的生物素(Biotin)标记的β-Catenin/TCF consensus oligonucleotide。

这个生物素标记的双链寡核苷酸含有公认的β-Catenin/TCF结合位点，可以用作EMSA研究时的探针。

β-Catenin/TCF consensus oligo的序列如下：5'-CCC TTT GAT CTT ACC-3'3'-GGG AAA CTA GAA TGG-5'本生物素标记EMSA探针已经过纯化，可以直接用于EMSA结合反应。

本生物素标记EMSA探针可以和碧云天的化学发光法EMSA试剂盒(GS009)配套使用。

一个包装的生物素标记探针可以进行约200-400个样品的EMSA检测。

包装清单：产品编号产品名称包装GS018B 生物素标记EMSA探针－β-Catenin/TCF (0.2µM) 200µl—说明书1份保存条件：-20ºC保存，一年有效。

注意事项：避免加热到40ºC以上，温度过高会导致双链DNA探针解聚成单链。

而单链无法用于EMSA研究。

对于基于生物素标记的EMSA检测的详细操作可以参考碧云天的化学发光法EMSA试剂盒(GS009)的使用说明。

本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

mce铁死亡脂质过氧化实验步骤MCE铁死亡脂质过氧化实验步骤：MCE铁死亡脂质过氧化实验是一种用于研究细胞脂质过氧化损伤的常用实验方法。

以下是MCE铁死亡脂质过氧化实验的基本步骤：1. 细胞处理：-准备细胞：选择需要研究的细胞系，将其培养至适当的生长状态。

-细胞处理：将细胞分为试验组和对照组。

试验组接受处理，包括暴露于一定浓度的铁离子(Fe2+)和添加氧化剂，如过氧化氢(H2O2)。

-对照组设定：设置一个对照组，不接受任何处理，以用于对比和分析实验结果。

2. 实验处理：-铁离子处理：将试验组的培养基中添加一定浓度的铁离子(Fe2+)，使其与细胞产生接触。

-氧化剂处理：在铁离子处理后，添加所需浓度的氧化剂，如过氧化氢(H2O2)。

-控制组处理：对照组只添加培养基，不加入铁离子和氧化剂。

3. 孵育条件：-孵育时间：将细胞培养在适宜的孵育温度下，根据实验要求确定孵育时间，通常为数小时至数天。

-适宜条件：提供适当的气体组成、湿度和培养基成分来维持细胞的正常生长。

4. 检测脂质过氧化：- TBARS测定：采用thiobarbituric acid reactive substances (TBARS)法检测脂质过氧化产物。

将细胞产生的上清液和合适的试剂混合反应，形成染色物质，并用光谱仪或分光光度计测定其吸光度，从而定量脂质过氧化水平。

-细胞活性：使用细胞存活检测试剂盒如MTT(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide)或CCK-8 (Cell Counting Kit-8)来测定细胞存活率，以评估细胞的受损程度。

5. 数据分析：-实验重复：进行足够的实验重复，以获得可靠的结果。

-数据统计分析：对实验结果进行统计学分析，如平均值和标准差计算，t检验或方差分析等。

MCE铁死亡脂质过氧化实验通过模拟细胞内的脂质过氧化反应，提供一种可靠的方法来研究细胞脂质损伤的机制和影响因素。

线粒体呼吸链复合体Ⅳ/细胞色素C 氧化酶活性检测试剂盒说明书微量法注意：本产品试剂有所变动，请注意并严格按照该说明书操作。

货号：BC0945规格：100T/96S产品组成：使用前请认真核对试剂体积与瓶内体积是否一致，有疑问请及时联系索莱宝工作人员。

试剂名称规格 保存条件 提取液液体75 mL×2瓶 2-8℃保存 试剂一液体33mL×1瓶 2-8℃保存 试剂二粉剂×2瓶 -20℃保存 试剂三粉剂×2支 2-8℃保存溶液的配制：1、 试剂二：试剂放于试剂瓶内玻璃瓶中。

临用前取1支加入13.5mL 试剂一溶解，用不完的试剂-20℃分装保存2周，避免反复冻融；2、 试剂三：试剂置于试剂瓶内EP 管中；临用前取1支加入2mL 试剂一溶解，用不完的试剂-20℃保存2周，避免反复冻融；3、 工作液的配制：临用前取0.5mL 试剂三加入到溶解好的4.5mL 试剂二中混合备用（约25T ），或者按比例现用现配。

产品说明：线粒体复合体Ⅳ又称细胞色素C 氧化酶，也是线粒体呼吸电子传递链主路和支路的共有成分，负责催化还原型细胞色素C 的氧化，并最终把电子传递给氧生成水。

还原型细胞色素C 在550nm 有特征光吸收，线粒体复合体Ⅳ催化还原型细胞色素C 生成氧化型细胞色素C ，因此550nm 光吸收下降速率能够反映线粒体复合体Ⅳ酶活性。

Reduced Cytochrome C （550nm ） Oxidized Cytochrome C注意：实验之前建议选择2-3个预期差异大的样本做预实验。

如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。

需自备的仪器和用品：可见分光光度计/酶标仪、台式离心机、水浴锅/恒温培养箱、可调式移液器、微量玻璃比色皿/96孔板、研钵/匀浆器/细胞超声破碎仪、冰和蒸馏水。

操作步骤：一、样本处理（可适当调整待测样本量，具体比例可以参考文献）1. 称取约0.1g 组织或收集500万细胞，加入1.0 mL 提取液，用冰浴匀浆器或研钵匀浆。

Inhibitors, Agonists, Screening LibrariesSafety Data SheetAug.-9-2018Aug.-9-20181. PRODUCT AND COMPANY IDENTIFICATION.1 Product identifier1Product name :Anti-c-Myc Magnetic Beads Catalog No. :HY-K0206 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data pany :MedChemExpress USA Tel :609-228-6898Fax :609-228-5909sales@E-mail :1.4 Emergency telephone number.609-228-6898Emergency Phone #:2. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms :Formula :Molecular Weight :CAS No. : /Anti-c-Myc Magnetic Beads / /4. FIRST AID MEASURES4.1 Description of first aid measurestc a t n o c e y E Remove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.o c n i k S tc a t n Rinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.Revision Date:Print Date:MedChemExpressI n h a l a t i o nImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.I n g e s t i o nWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling, see section 2.2.4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated ma terial according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Store at 4°C 2 yearsShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parameterss t n e n o po Ch t i wma r a p l o r t n o c e c a l p k r os r e t emwThis product contains no substances with occupational exposure limit values.8.2 Exposure controlssl o r t n o c g n i r e e n i g n E Ensure adequate ventilation. Provide accessible safety shower and eye wash station.tn e m p i u q e e v i t c e t o r p l a n o s r e P no i t c e t o r p e y E Safety goggles with side-shields.no i t c e t o r p d n a H Protective gloves.no i t c e t o r p y d o b d n a n i k S Impervious clothing.no i t c e t o r p y r o t a r i p s e R Suitable respirator.n e m n o r i v n E s l o r t n o c e r u s o p x e l a t Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesec n a r a e p p A Brown (Liquid Mixture)ro d O No data available dl o h s e r h t r o d O No data available Hp No data available tn i o p g n i z e e r f /g n i t l e M No data available eg n a r /t n i o p g n i l i o B No data available tn i o p h s a l F No data available et a r n o i t a r o p a v E No data available )s a g ,d i l o s ( y t i l i b a m m a l F No data available st i m i l e v i s o l p x e r o y t i l i b a m m a l f r e w o l /r e p p U No data available er u s s e r p r o p a No data available yt i s n e d r o p a No data available yt i s n e d e v i t a l e R No data available yt i l i b u l o S r e t a W No data available tn e i c i f f e o c n o i t i t r a P No data available er u t a r e p m e t n o i t i n g i -o t u A No data available er u t a r e p m e t n o i t i s o p m o c e D No data available yt i s o c s i No data available se i t r e p o r p e v i s o l p x E No data available s e i t r e p o r p g n i z i d i x O No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsy t i c i x o t e t u c AClassified based on available data. For more details, see section 2.n i k Sn o i t a t i r r i/n o i s o r r o cClassified based on available data. For more details, see section 2.e y e s u o i r e Sa dn o i t a t i r r i/e g amClassified based on available data. For more details, see section 2.y r o t a r i p s e Rn o i t a z i t i s n e s n i k s r oClassified based on available data. For more details, see section 2.m r e Gl l e cy t i c i n e g a t umClassified based on available data. For more details, see section 2.y t i c i n e g o n i c r a CIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.y t i c i x o t e v i t c u d o r p e RClassified based on available data. For more details, see section 2.e l g n i s-y t i c i x o t n a g r o t e g r a t c if i c e p Se r u s o p x eClassified based on available data. For more details, see section 2.d e t a e p e r-y t i c i x o t n a g r o t e g r a t c i f i c e p Se r u s o p x eClassified based on available data. For more details, see section 2.n o i t a r i p s A d r a z a hClassified based on available data. For more details, see section 2.12. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsP r o d u c tDispose substance in accordance with prevailing country, federal, state and local regulations.C o n t a m i n a t e d p a c k a g i n gConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIOND O T(U S)This substance is considered to be non-hazardous for transport.I M D GThis substance is considered to be non-hazardous for transport.I A T AThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONS A R A302C o m p o n e n t s:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.S A R A313C o m p o n e n t s:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.S A R A311/312H a z a r d s:No SARA Hazards.M a s s a c h u s e t t s R i g h t T o K n o w C o m p o n e n t s:No components are subject to the Massachusetts Right to Know Act.P e n n s y l v a n i a R i g h t T o K n o w C o m p o n e n t s:No components are subject to the Pennsylvania Right to Know Act.N e w J e r s e y R i g h t T o K n o w C o m p o n e n t s:No components are subject to the New Jersey Right to Know Act.C a l i f o r n i a P r o p.65C o m p o n e n t s:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@MedChe Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

23620描述ProFound™ c-Myc Tag IP/Co-IP Kit 包含了足够25个与c-Myc –标签蛋白IP或Co-IP的成分，包括10个c-Myc –标签阳性对照的实验试剂盒成分：Immobilized Anti-c-Myc, 62.5 g固定的珠状琼脂加上125g 抗体，以含25%的溶液和0.1%叠氮钠的PBS 的形式提供(总体积为250ul)。

BupH™ Tris Buffered Saline Pack,一个包装，用500 ml超纯水稀释后，溶液成分为25 mM Tris, 0.15 M NaCl, pH 7.2Elution Buffer, 50 ml, pH 2.8Lane Marker Non-Reducing Sample Buffer (5X), 5 ml, 包含0.3 M Tris•HCl, pH 6.8, 5% SDS, 50% 甘油, 电泳指示剂。

Handee™ Spin Columns Accessory Pack, 含提前放置好的玻璃原料，顶盖和底盖的27Collection Tubes and Caps Accessory Pack, 100 有刻度的2 ml 管子和塞盖c-Myc-tagged Positive Control, 500ul，1 mg/ml E. coli 提取的包含c-Myc-标签GST（古胱氨酸S-转移酶）储藏: 收到后，将c-Myc-标签阳性对照放在-80度冰箱，（在-20度可放6个月），将其他所用到的成分放在4度，c-Myc-标签阳性对照用干冰运输。

注意：ProFound™ c-Myc Tag IP/Co-IP Kit是ProFound™ c-Myc Tag IP/Co-IP Application Set (23622) 和c-Myc-tagged Positive Control (23633)的联合体。

介绍ProFound™ c-Myc Tag IP/Co-IP Kit与用蛋白A/G琼脂糖的IP方法相比，传统的提供了一个简单快速的方法来研究c-Myc-标签蛋白。

Anti-c-Myc Affinity Beads目录1. 产品介绍 (1)2. 试剂准备 (1)3. 样品纯化 (1)4. 问题及解决方案 (2)5. 订购信息及相关产品 (3)1. 产品介绍Anti-c-Myc Affinity Beads可以检测原核、真核表达的c-Myc标签蛋白融合蛋白。

c-Myc 多肽是由人类染色体8q24上的c-Myc基因编码，对应于人类P62的410-419（EQKLISEEDL）氨基酸。

Anti-c-Myc Affinity Beads可以识别C端，N端或内部c-Myc 标签蛋白融合蛋白，可应用在Western-blot杂交技术、免疫沉淀和流式细胞计量术中, 用于检测重组蛋白质在靶细胞中的表达。

表1. Anti-c-Myc Affinity Beads产品性能指标性能基质4%琼脂糖微球配体Anti-c-Myc鼠单克隆抗体载量>1mg c-Myc标签蛋白/ml 介质粒径(μm)45-165最大压力0.1 MPa, 1 bar储存缓冲液0.02%叠氮化钠，1XPBS储存温度2°C - 8°C2. 试剂准备2.1缓冲液的准备所用水和缓冲液在使用之前建议用0.22μm或0.45 μm滤膜过滤。

平衡/洗杂液: 50mM Tris, 0.15M NaCl, pH7.4洗脱液: 0.1M Glycine HCl, pH3.0竞争性洗脱液：50mM Tris, 0.15M NaCl, 100µg c-Myc多肽/ml，pH7.4中和液：1M Tris-HCl，pH8.52.2 样品准备上柱之前要确保样品溶液有合适的离子强度和pH值，可以用平衡液对样品或细胞培养液稀释，或者用平衡液透析。

样品在上样前建议离心或用0.22μm或0.45μm滤膜过滤，减少杂质，提高蛋白纯化效率和防止堵塞柱子。

3. 样品纯化3.1 柱层析1) 将Anti-c-Myc Affinity Beads装入合适的层析柱，用5 倍柱体积的平衡液进行平衡，使填料处于与目的蛋白相同的缓冲体系下。