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BioPM抗体汇总表2015

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鼠抗人CD20单克隆抗体的制备与鉴定

鼠抗人CD20单克隆抗体的制备与鉴定

万方数据交瘸上清并进行克隆化。

１．２．３ｗｅｓｔｅｍｂｌｏｔ分析用２×ＳＤＳ上样缓冲液悬浮制备的ＨｅＬａ细胞总蛋白，于９５℃加热７Ｉｎｉｎ，置于冰上冷却１０ⅡＩｉｎ。

上样量为５峭，经ｓＤｓ．ＰＡＧＥ分离后，以湿转法电转至硝酸纤维素膜上。

经脱脂奶粉封闭（室温ｌｈ）后，依次滴加杂交瘤细胞上清（４℃过夜。

以１ｍＬ／ＬＴｗｅｅｎ２０’ｒＢＳ洗膜３次）及１：５０００ＨＲＰ山羊抗小鼠ＩｇＧ（室温孵育ｌｈ，以ｌｍＬ／Ｌ’ｒｗｅｅＩｌ２０ｒｒＢＳ洗膜３次）。

用化学发光剂试剂盒（ＥＣＬＰｌ璐）处理５ｍｉｎ后于暗室曝光到Ｘ胶片上，显影、定影。

１．２．４免疫荧光鉴定将ＨｅＩＪａ细胞接种于培养皿中进行细胞爬片，ＰＢＳ洗标本，４∥Ｌ多聚甲醛固定１０ＩＩｌｉｎ；ＰＢｓ洗标本３遍，羊血清封闭液封闭，一抗孵育（细胞培养上清原液）４℃过夜，ＰＢＳ洗标本３遍，滴加ＦＩＴＣ标记的羊抗小鼠二抗（１：２００稀释），ＰＢｓ洗标本３遍，Ｈｏｃｈ船ｔ染核，ＰＢＳ洗标本后用蒸馏水冲洗１遍，８００ｍＬ／Ｌ甘油封片，镜检，拍照。

１．２．５人子宫内膜中抗原的免疫组化染色检测取出石蜡包埋的人子宫内膜组织切片，脱蜡，将切片浸于３００Ⅱ∥ＬＨ：Ｏ：（１：５０）液中，于室温浸泡３０ｎｌｉｎ灭活内源性过氧化物酶，以蒸馏水洗３次。

杂交瘤上清４℃孵育过夜。

ＰＢｓ洗片后加入羊抗小鼠ＩｇＧ．ＨＲＰ，３７℃孵育２０ｒｎｉｎ，ＰＢＳ洗片后进行ＤＡＢ显色，苏木素复染，返蓝后，脱水、透明、封片，镜检，放大４００倍拍照记录结果。

２结果２．１ＣⅡ加目的片段的扩增琼脂糖凝胶电泳显示在２４５ｂｐ处有一条特异的扩增条带，片段大小与预期值一致，说明ＰｃＲ特异性好，扩增成功（图１）。

图ｌＰｃＲ扩增产物的凝胶电泳分析１：ＤＮＡ叫Ｉｒｋ凹（ＤＬ２０００）；２：ＣＤ２０．２．２ＣＤ狮融合蛋白的表达与鉴定经矾Ｂ诱导后，ＣＤ２０－ＧＳＴ和ｃＤ２０－Ｈｉｓ在大肠杆菌ＢＬ２ｌ中大量表达，ＪＩ，，分别为３５０００和２９０００，融合蛋白均为可溶性表达，经过柱纯化后纯度可达９０％以上（图２）。

6840 体外诊断试剂分类子目录汇编

6840 体外诊断试剂分类子目录汇编

6840 体外诊断试剂分类子目录（2013版）序号产品类别产品分类名称预期用途管理类别001 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂志贺菌属多价诊断血清临床上用于志贺菌属分群。

Ⅲ002 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂鲍氏志贺菌诊断血清临床上用于鲍氏志贺菌群分型。

Ⅲ003 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂福氏志贺菌诊断血清临床上用于福氏志贺菌群分型。

Ⅲ004 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂宋内志贺菌诊断血清临床上用于宋内志贺菌诊断。

Ⅲ005 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂志贺志贺菌诊断血清临床上用于志贺志贺菌诊断。

Ⅲ006 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂变形杆菌OX19诊断菌液临床上用于外斐（Weil－Felix）反应。

Ⅲ007 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂变形杆菌OX2诊断菌液临床上用于外斐（Weil－Felix）反应。

Ⅲ008 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂变形杆菌OXK诊断菌液临床上用于外斐（Weil－Felix）反应。

Ⅲ009 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂甲型副伤寒杆菌诊断菌液临床上用于肥达(Widal)反应。

Ⅲ010 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂乙型副伤寒杆菌诊断菌液临床上用于肥达(Widal)反应。

Ⅲ011 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂丙型副伤寒杆菌诊断菌液临床上用于肥达(Widal)反应。

Ⅲ012 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂伤寒杆菌H901诊断菌液临床上用于肥达(Widal)反应。

Ⅲ013 Ⅲ-1 与致病性病原体抗原、抗体以及核酸等检测相关的试剂伤寒杆菌O901诊断菌液临床上用于肥达(Widal)反应。

碧云天(beyotime)生物产品代理目录价格表(2015年)

碧云天(beyotime)生物产品代理目录价格表(2015年)

生物素3' 末端DNA标记试剂盒 生物素随机引物DNA标记试剂盒 D3308 化学发光法生物素标记核酸检测试剂盒 D3308B 封闭液(D3308专用) D3308W 洗涤液(5X，D3308专用)
产品名称
内切酶 产品编号 D6049 D6053 D6093 D6257 D6329 D6330 D6337 D6389 D6390 D6417 D6449 D6481 D6485 D6489 D6497 D6565 D6566 D6581 D6585 D6593 D6597 D6633 D6713 D6721 修饰酶 产品编号 D7012 D7021 D7027 D7035 D7039 D7051 D7062 D7066 D7069 D7073
1000U DNase I 100U RNase H Terminal Deoxynucleotidyl Transferase 500U 100U T4 Polynucleotide Kinase 500U T4 Polynucleotide Kinase 2000U BeyoRT M-MuLV反转录酶 2000U BeyoRT M-MuLV反转录酶(RNase H-) 产品名称 产品包装 2000U
ApaI BamHI BglII DpnI EcoRI EcoRI EcoRV HindIII HindIII KpnI MluI NcoI NdeI NheI NotI PstI PstI PvuII RsaI SacI SalI SmaI XbaI XhoI
产品名称
DNA末端平滑试剂盒 T4 RNA Ligase BeyoAP Alkaline Phosphatase Klenow Fragment Klenow Fragment，ExoT4 DNA Polymerase SP6 RNA Polymerase T3 RNA Polymerase T7 RNA Polymerase DNase I

碧云天 GFP抗体(小鼠单抗)说明书

碧云天 GFP抗体(小鼠单抗)说明书

碧云天生物技术/Beyotime Biotechnology 订货热线：400-168-3301或800-8283301 订货e-mail ：****************** 技术咨询：***************** 网址：碧云天网站 微信公众号GFP 抗体(小鼠单抗)产品编号 产品名称包装 AG281GFP 抗体(小鼠单抗)>40次产品简介：来源 用途 抗体识别位点抗体类型 GFP 分子量MouseWB, IP, IFFull length GFP IgG2a~27kDWB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence. 本GFP 抗体(小鼠单抗)，即mouse monoclonal GFP antibody ，为进口分装，用全长的来源于水母的GFP (green fluorescent protein)作为抗原制备得到的抗GFP 小鼠单克隆抗体。

克隆号为B-2。

本GFP 抗体可以识别GFP以及GFP 的一些突变体例如EGFP (enhanced green fluorescent protein)。

GFP 或其突变体EGFP 等被广泛用于基因表达效率的检测，以及和目的蛋白融合表达用于检测目的蛋白的表达和分布。

本GFP 抗体不仅可以检测GFP 或其适当的突变体，也可以检测和GFP 或其适当的突变体融合表达的蛋白。

配套提供了Western 一抗稀释液，可以用于Western 检测时的一抗稀释。

)：40次。

包装清单：产品编号 产品名称包装 AG281-1 GFP 抗体(小鼠单抗) 40µl AG281-2 Western 一抗稀释液40ml —说明书1份保存条件：GFP 抗体(小鼠单抗)-20ºC 保存，Western 一抗稀释液-20ºC 或4ºC 保存，一年有效。

高表达抗PD-1抗体CHO细胞株的筛选及代谢分析

高表达抗PD-1抗体CHO细胞株的筛选及代谢分析

高表达抗PD-1抗体CHO细胞株的筛选及代谢分析梅文枫;朱一平;尹帮旗;许晓刚;方树彬【摘要】目的筛选高表达抗PD-1抗体的工程细胞株和较优的基础及补料培养基,并进行代谢分析. 方法分别在TPP摇管、摇瓶中通过流加培养的方式,从6株细胞株和5种商业培养基中筛选高表达抗PD-1抗体的GS-CHO细胞株和较优的基础及补料培养基.在流加培养过程中,检测葡萄糖、谷氨酰胺、谷氨酸、乳酸及氨等的浓度变化.同时为分析限制性营养因素,进一步对乳酸、氨及氨基酸代谢进行分析. 结果通过TPP摇管初步筛选,显示122和126两株细胞在HyCellCHO培养基中表现最好,表达量分别为1.945和1.989 g/L;在摇瓶中进一步验证显示122细胞在6株细胞中的最大活细胞密度图最高,达2.21×107 cells/mL,表达量＞2 g/L.代谢分析发现,乳酸代谢切换和谷氨酸及丙氨酸代谢相关.氨基酸分析发现,蛋氨酸、异亮氨酸、亮氨酸等必需氨基酸可能为122细胞株生长和(或)表达限制性成分. 结论筛选出高表达抗PD-1抗体的细胞株和较优培养基,同时代谢分析和氨基酸分析为后续细胞培养工艺开发及个性化培养基优化提供理性指导.【期刊名称】《福建医科大学学报》【年(卷),期】2018(052)005【总页数】8页(P320-327)【关键词】GS-CHO细胞;培养基;细胞计数;代谢【作者】梅文枫;朱一平;尹帮旗;许晓刚;方树彬【作者单位】福建医科大学免疫治疗研究所,福州350122;福建医科大学免疫治疗研究所,福州350122;福建医科大学免疫治疗研究所,福州350122;福建医科大学免疫治疗研究所,福州350122;福建医科大学免疫治疗研究所,福州350122【正文语种】中文【中图分类】R329.24;R341;R977.6程序性死亡蛋白1(programmed death 1，PD-1)信号通路成为肿瘤免疫治疗的热点之一，肿瘤细胞表面的程序性死亡配体1(programmed death ligand 1，PD-L1)通过与PD-1的结合抑制了肿瘤特异性CD8+T细胞的免疫活性，介导了免疫逃逸，抗PD-1/抗PD-L1抗体通过阻断负性免疫调节信号，阻断免疫逃逸而杀伤肿瘤[1]。

流式点阵免疫发光法检测抗染色质抗体在结缔组织病检测中的价值探析

流式点阵免疫发光法检测抗染色质抗体在结缔组织病检测中的价值探析

流式点阵免疫发光法检测抗染色质抗体在结缔组织病检测中的价值探析【摘要】目的：观察分析在结缔组织病中采用流式点阵免疫发光法实际检测效果。

方法：随机选择在我院医治的结缔组织病患者100例，设为观察组，随机选择在我院医治的非风湿病患者100例，设为对照组，使用流式点阵免疫发光法检测抗染色质抗体，经观察对比抗染色质抗体阳性率，得出结论。

结果：对于各项数据的对比来说，观察组的各项数据更加有优势，差异有统计学意义（P＜0.05）。

观察组抗染色质抗体阳性率高于对照组，差异有统计学意义（P＜0.05）。

结论：对于结缔组织病采用流式点阵免疫发光法检查抗染色质抗体，可以实际检出更高的抗染色质抗体阳性率，因此值得推广采纳。

【关键词】结缔组织病；抗染色质抗体；核流式点阵免疫发光法自身抗体对于结缔组织病的鉴别诊断至关重要。

抗染色质抗体是由核中的染色体作为自身抗原产生的抗体，是诊断系统性红斑狼疮的特征性自身抗体[1-2]。

近年来，全自动流式点阵免疫发光技术逐渐应用于临床，该技术采用高通量检测技术，联合检测多种自身抗体，实现定量分析，一次完成多个指标的检测，检测时间大大缩短。

本文即分析在结缔组织病中采用流式点阵免疫发光法检测抗染色质抗体具体效果，主要内容见下文：1 资料与方法1.1一般资料随机选择我院于2021年1月至2021年12月内收治的100例结缔组织病患者，设为观察组，年龄范围：31-73岁，平均年龄：（54.44±20.91）岁；随机选择100例非风湿病患者为研究对象，设为对照组，年龄范围：32-74岁，平均年龄：（54.81±20.55）岁。

使用流式点阵免疫发光法检测抗染色质抗体，对检测结果进行收集和分析。

所有患者文化程度、年龄、身高等基础资料分析后显示，其差异无统计学意义（P＞0.05）。

1.2方法两组实施抗染色质抗体检测，内容如下：使用Bio-Plex2200全自动流式点阵免疫发光分析仪（美国伯乐公司）及配套Bio-Plex ANA Screen 11项检测试剂盒（流式点阵免疫发光法）、校准品及质控品检测抗染色质抗体。

阿尔茨海默病患者Aβ142抗体测定的临床意义

阿尔茨海默病患者Aβ142抗体测定的临床意义

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ｌｅｖｅｌｓ ｉｎ ＡＤ ｐａｔｉｅｎｔｓ ａｒｅ ｓｉｇｎｉｆｉｃａｒＩｔｌｙ ｌｏｗｅｒ ｔｈａｎ ｔｈｏｓｅ ｉｎ ｎｏｎｎａｌ ｐｅｏｐｌｅ． Ｄｅｃｒｅａｓｅｄ ｓｅｌｌｌｍ Ａ８ｌ啦锄ｔｉｂｏｄｙ
５．Ａｐ㈨自身抗体竞争ＥＬＩｓＡ法评价：在人血清 中加Ａｐ。４：蛋白片段包被的聚苯乙烯磁珠，将Ａｐ，ｍ 自身抗体进行吸附分离，然后检测吸附后的血清。 另外，由于马血清不含Ａｐ，啦自身抗体旧Ｊ，采用同一
万方数据
方法检测马血清中ＡＢ抗体。 ６．统计学分析：ＡＤ组及健康对照组血清Ａｐ。ｍ
自身抗体浓度用元±ｓ表示，组间比较采用双样本成 组等方差￡检验，Ｐ＜０．０５为差异有统计学意义。
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ｎｏＨＩｌａｌ ｐｅｏｐｌｅ［（１２．６±３．３）ｎ∥ｌＴｌｌ，Ｐ＜Ｏ．０１］ｏｒ ｉｎ ｂｒａｉｎ ｔｌｌｒｏＩｌｌｂｕｓ ｐａｔｉｅｎｔｓ［（１２．５±３．３）ｎ∥ｍｌ，￡＝
ｌｅｖｅｌｓ ｉｎ ＡＤ ｐａｔｉｅｎｔｓ ｉｎｄｉｃａｔｅ ｔｌｌａｔ ｔｈｅｉｒ ｃｌｅａｒａｎｃｅ ａｂｉｌｉｔｙ ｏｆ ＡＢ ｉｓ ｎｏｔ ｅｎｏｕｇｈ． 【Ｋｅｙ ｗｏｒｄｓ】 Ａｎｔｉｂｏｄｙ，ｍｏｎｏｃｌｏｎａｌ； Ａｌｚｈｅｉｍｅｒ’ｓ ｄｉｓｅａｓｅ； Ｂ—ａｍｙｌｏｉｄ
阿尔茨海默病（ＡＤ）为一种中枢神经系统原发 性退行性病变，患者进行性智力和认知功能降低，其 在老年人中的发病率达２‰。老年斑（ｓＰ）和神经元 纤维缠结（Ｎ丌）是ＡＤ的主要病理改变。Ｂ淀粉样 蛋白（ＡＢ）是ｓＰ的主要成分，并参与ＮＦｒｌｌ的形成。 ＡＢ为含有３９４３个氨基酸的多肽，由其淀粉样前体 蛋白（ＡＰＰ）裂解产生，ＡＤ患者ＳＰ主法标准曲线：用马 血清及含ＡＢ。越蛋白片段的聚苯乙烯磁珠吸附后的 人血清ＡＢ，越自身抗体含量均低于１ ｎ∥１１１ｌ。将兔 抗Ａｐ，ｍ多价抗体作系列稀释至Ｏ．０１、０．２、ｌ、５、２５、 １２５、６２５ ｎ∥ｒｎｌ的测定结果显示，当浓度≥１ ｎｇ／ｒｎｌ 时，其浓度与Ａ值呈半对数线性关系，表明该检测 方法灵敏度符合临床检测要求（图１）。

DNA实验-免疫细胞表面抗原分子CD家族对照表

DNA实验-免疫细胞表面抗原分子CD家族对照表

D N A实验-免疫细胞表面抗原分子C D家族对照表C D1-C D24(总9页) -本页仅作为预览文档封面，使用时请删除本页-免疫细胞表面抗原分子CD家族对照表(CD1-CD247)Exalpha Biologicals produces and sells products for Life Science Research, including monoclonal and polyclonal antibodies, for use in immunohistochemistry, western blot, ELISA and Flow Cytometry. We offer over 800 products and, through collaborations with other scientists as well as our own in-house R&D programs, Exalpha continues to bring leading edge products to market. Our family of CD antibodies combines quality and performance with reasonable prices to make our CD antibodies the best value in the market today. Our family of Proliferation products offer easy all-in-one kits or stand alone antibodies to quantitate proliferating cells. Take a look at our website or call us for additional information.Note: no information is available on CDw145, CD181, CD182, CD185-194, CD196-199, CD211, CD214-216, CD218-220, CD237 BackgroundCD: cluster designation of monoclonal antibodies (clusters of differentiation)Designated at the 1st to 7th Workshops on International Human Leukocyte Differentiation Antigens The last conference was in 2000. The next conference is in 2004.HLDA Workshops are the primary mechanism to characterize leukocyte surface antigenic molecules and epies; erythroid antigens are now includedFor 1st to 6th workshops, antibodies were submitted to the organizing laboratory, coded and sent to participating laboratories for testing against various cell types. For the 7th workshop, a CD designation could be established for a molecule if its gene has been cloned and at least one specific monoclonal antibody had been studied in the WorkshopInterpretation should be based on cellular distribution of staining, proportion of positively stained cells, staining intensity and cutoff levels.CD1Family of non-polymorphic MHC class I-like glycoproteinsAlso member of immunoglobulin superfamilyOn chromosome 1q22-23 (not MHC linked)Has 5 different subsets, all noncovalently associated with 12 kd beta 2 microglobulinFunction: restrict T cell responses to certain antigens; may mediate thymic T cell developmentPositive staining (normal): cortical thymocytes (70%), activated T cells, Langerhans cells, interdigitating dendritic cellsPositive staining (disease): pre T ALL with cortical thymocyte phenotype; Langerhans cell histiocytosisNegative staining: mature peripheral T cellsCD1aPositive staining (normal): Dendritic cells in dermis/epidermis of benign inflammatory skin disordersPositive staining (disease): Langerhans cell histiocytosis (fairly specific), myeloid leukemias, some B cell malignancies; dendritic cells in most peripheral cutaneous T cell lymphomas, AJCP 2001;116:72Negative staining: normal B cells, most cutaneous peripheral B cell lymphomas ( reflects replacement of reactive pattern containing dendritic cells with a neoplastic pattern of B cells)Micro images:Langerhans cell histiocytosisMicro images (AJSP subscribers): pulmonary Langerhans cell histiocytosisMicro images (Hum Path subscribers): Langerhans cell histiocytosisReferences: AJSP 2001;25:630CD1bPositive staining (disease): myeloid leukemias and some B cell malignanciesNegative staining: normal B cellsCD1cPositive staining (normal): subset of normal peripheral B cellsPositive staining (disease): myeloid leukemias and some B cell malignanciesNegative staining: normal B cellsCD1dPositive staining (normal): thymus (low levels), bowelCD1eCD2Aka E rosette receptor, LFA-2 (leukocyte function antigen)Function: binds CD58 / LFA-3 on antigen-presenting cells, and induces costimulatory signals in T cellsAlso regulates T and NK-mediated cytolysis, inhibits apoptosis of activated peripheral T cells, mediates T cell cytokine production, regulates T cell anergyPositive staining (normal): thymocytes (95%), mature peripheral T cells (almost all), NK cells (80-90%), thymic B cells (50%)Micro images:extranodal NK/T cell lymphoma, nasal typeCD2RCD2 epies restricted to activated T cellsPositive staining: activated T cells, NK cellsCD3Aka OKT3Function: complex (5 chains) of integral membrane glycoproteins assembled as a complex; has long cylasmic tail with antigen recognition activation motif; complex is then down regulatedAlso subdivided into delta, epsilon, gamma subtypesCylasmic expression at early T cell differentiation, then membranous expressionMost specific T cell antibodyPositive staining (normal): thymocytes, peripheral T cells, NK cells; also Purkinje cells of cerebellumPositive staining (disease): 80% of T cell lymphomasNegative staining: gamma delta T cell receptors, most B cell lymphomasMicro images:CD3 epsilon-testicular NK/T cell lymphoma (figure 3D)Micro images (AJSP subscribers): achalasia, post-transplant lymphoproliferative disease in liver References: AJSP 2001;25:1413CD4Aka OKT4, T helper/inducerOn chromosome #12pNonpolymorphous glycoproteins belonging to immunoglobulin superfamilyServes as HIV receptor on T cells (as do chemokine receptors CCR5 and CXCR4), macrophages, brain CD4+ T cells are killed by HIVCoreceptor in MHC class II-restricted antigen induced T cell activationBinds to nonpolymorphic region of class I molecules; may increase avidity of cell-cell interactionsPositive staining (normal): thymocytes (80-90%), mature T cells (65%, T helper and CD4/CD8+ thymocytes), macrophages, Langerhans cells, dendritic cells, granulocytesPositive staining (disease):pityriasis lichenoidesMicro images: acute demyelinating disease, extranodal NK/T cell lymphoma, nasal typeCD5Belongs to ancient scavenger receptor familyIs physically and functionally coupled with T cell receptor-zeta-CD3 signal transducer complexCD5+ B cells produce “generalist antibodies” - polyreactive low affinity “natural” antibodies to exogenous antigens (tetanus toxoid, lipopolysaccharide) as well as autoreactive antibodies (ssDNA, thyroglobulin, insulin)Note: sharks only have polyreactive IgMNote: monoreactive IgG is produced by < % of circulating B cells, from positive selection and somaticpoint mutationFirst line of defense against antigens; have a low activation threshold; are the only line of defense for those who cannot produce specific antibodyProduce antibodies using germ line (non mutated) configuration of gene segments, usually IgMProduction elevated in rheumatoid arthritis (27-52% of circulating B cells vs. 20% normal)CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and the development stagePositive staining (normal): B cells of mantle zone of spleen and lymph nodes; B cells in peritoneal and pleural cavities; almost all T cells;In fetus, most B cells in spleen and cord blood are CD5 positivePositive staining (disease): B cell CLL/SLL, mantle cell lymphoma, most T malignancies, thymic carcinomas (70%)Negative staining: spindle cell thymomas, MALT lymphoma, follicular lymphomaMicro images: extranodal NK/T cell lymphoma, nasal type, mantle cell lymphoma (figure 3D)CD6Adhesion molecule mediating the binding of developing thymocytes with thymic epithelial cellsMay be involved in autoimmunity and graft vs. host disease (GVHD)Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent GVHD Positive staining (normal): low levels on immature thymocytes, high levels on mature thymocytesCD7Membrane glycoprotein and Fc receptor for IgMHomologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearrangement, persists until terminal stages of T cell developmentLower expression in memory T cells vs. naive T cellsPositive staining (normal): mature peripheral T cells (85%), post-thymic T cells (majority), NK cells (majority), some myeloid cellsPositive staining (disease): T cell ALL; AML (especially M4/M5), chronic myelogenous leukemia, blasts in transient myeloproliferative disorderNegative expression: B cell ALL, Sezary syndrome, adult T cell leukemia/lymphomaMicro images: extranodal NK/T cell lymphoma, nasal typeCD8Aka OKT8, T cell suppressor/cytotoxic cellsOn chromosome #2MHC class I restricted receptor; binds to nonpolymorphic region of class I molecules; may increase avidity of cell-cell interactionsAssociated with lymphoepithelioma-like carcinoma of lung (AJSP 2002;26:715)Positive staining (normal): T cells (25-35% of mature peripheral T cells, most cytotoxic T cells,CD4/CD8+ thymocytes); NK cells (30%-which are also CD3 negative); cortical thymocytes (70-80%), epidermotrophic lymphocytes in mycosis fungoides (AJSP 2002;26:450)Micro images: lymphoepithelioma-like carcinoma of cervix-figure 3, acute demyelinating diseaseMicro images (Mod Path subscribers): nodal cytotoxic T cell lymphomaReference: Mod Path 2002;15:1131CD9May mediate platelet activation and aggregationAntibodies are used to purge bone marrow prior to peripheral stem cell bone marrow transplantViral co-receptorPositive staining (normal): pre B cells, B cell subset, T cells, macrophages, platelets, eosinophils, basophils, megakaryocytes, endothelial cells, brain, peripheral nerve, vascular smooth muscle, cardiac muscle, epitheliaCD10Aka C ommon A cute L ymphoblastic L eukemia A ntigen (CALLA), neutral endopeptidase , neprilysin, enkephalinaseCell membrane metallopeptidase, characteristic marker of follicular center cells and follicular lymphoma, but also widely distributed in normal tissue and neoplasms; also localized to brush border in small bowel mucosaInactivates bioactive peptidesUses:Acute lymphoblastic leukemia: one of first markers to identify leukemic cells in children (hence its name)Breast: marker of myoepithelial cells, Mod Path 2002;15:397Burkitt lymphoma: confirm diagnosisColonic carcinogenesis: increase in stromal cells from mild to severe dysplasia to invasive carcinoma, Hum Path 2002;33:806-811Endometriosis: helpful in identifying areas of endometriosis if sparse glandular tissue。

免疫学抗体中英文对照表

免疫学抗体中英文对照表

免疫学B细胞膜表面免疫球蛋白 B cell surface membrane immunoglobulin B因子 B factorC反应蛋白 C reactive protein, CRPT3淋巴细胞亚群T3 lymphocyte subpopulationT4淋巴细胞亚群T4 lymphocyte subpopulationT淋巴细胞转化试验T lymphocyte transformation test阿米巴血清学试验ameba serological test白细胞杀菌功能试验leucocyte bactericidal function test 白细胞介素2可溶性受体soluble receptor of interleukin 2白细胞粘附抑制试验leucocyte adherence inhibition test包虫病血清学试验hydatidosis serologica test孢子丝菌病血清学试验sporotrichosis serological test丙型肝炎病毒核酸测定hepatitis C virus nucleic acid test补体1q complement 1q,C1q补体1r complement 1r,C1r补体1s complement 1s,C1s补体1抑制因子complement 1 inhibitor补体2 complement 2,C2补体3 complement 3,C3补体9 complement 9,C9补体受体Complement receptor,CR布氏杆菌凝集试验brucell agglutination test超敏反应，变态反应Allergy,hypersensitivity单纯疱疹病毒血清学试验herpes simplex virus serological test 丁型肝炎病毒核酸测定hepatitis D virus nucleic acid test肥达反应Widal reaction肥大细胞脱颗粒试验mastocyte degranulation test弗氏佐剂Freund's adjuvant副蛋白免疫学检查paraprotein immunoassay干扰素interferon钩端螺旋体病血清学试验leptospirosis serological test黑热病血清学试验kala-azar serological test鲎试验limulus test化支睾吸虫病血清学试验clonorchiosis serological test蛔虫病血清学试验ascariasis serological testpoliovirus serological test脊髓灰质炎病毒血清学试验甲胎蛋白alpha fetoprotein, AFP,α-FP巨噬细胞吞噬功能试验macrophage phagocytic function test聚合酶链反应polymerase chain reacton, PCR军团菌血清学试验legionella serological test抗EB病毒抗体anti-Epstein-Barr virus antibody抗IgA抗体anti-IgA antibody抗Q热抗体anti-Q fever antibody抗SS-A抗体，抗RO抗体anti-SS-A antibody, anti-RO antibody抗SS-B抗体anti-SS-B antibody抗白蛋白IgG抗体anti-albumin IgG antibody抗白蛋白IgM抗体anti-albumin IgM antibody抗斑疹伤寒抗体anti-typhus antibody抗甲型肝炎病毒IgG抗体anti-hepatitis A virus IgG antibody抗甲型肝炎病毒IgM抗体anti-hepatitis A virus IgM antibody抗丙型肝炎病毒抗体anti-hepatitis C virus antibody抗丁型肝炎病毒抗体anti-hepatitis D virus antibody抗乙型肝炎病毒e抗体anti-hepatitis B virus e antibody, HBeAb 又称抗HBe抗乙型肝炎病毒表面抗体anti-hepatitis B virus surface antibody,HBsAb又称抗HBs抗乙型肝炎病毒核心IgG抗体anti-hepatitis B virus core IgG antibody 又称抗HBc-IgG抗乙型肝炎病毒核心IgM抗体anti-hepatitis B virus core IgM antibody 又称抗HBc-IgM抗乙型肝炎病毒核心抗体anti-hepatitis B virus core antibody, HBcAb 又称抗HBc抗补体抗体anti-complement antibody抗单链DNA抗体anti-single-stranded DNA antibody抗风疹病毒IgG抗体anti-rubella virus IgG antibody抗风疹病毒IgM抗体anti-rubella virus IgM antibody抗副流感病毒IgG抗体anti-parainfluenza virus IgG antibody抗副流感病毒IgM抗体anti-parainfluenza virus IgM antibody抗肝细胞膜抗体anti-liver cell membrane antibody抗弓形体IgG抗体anti-toxoplasma IgG antibody抗弓形体IgM抗体anti-toxoplasma IgM antibody抗骨骼肌抗体anti-skeletal muscle antibody抗核抗体anti-nuclear antibody, ANA抗核因子anti-nuclear factor,ANF抗核仁抗体anti-nucleolus antibody抗核提取物抗体anti-nuclear extract antibody抗红细胞抗体anti-erythrocyte antibody抗呼吸道合胞病毒抗体anti-respiratory syncytial virus antibody抗甲状腺球蛋白抗体anti-thyroglobulin antibody抗甲状腺微粒体抗体anti-thyroid-microsome antibody抗结核抗体anti-tuberculosis antibody抗精子抗体anti-sperm antibody抗巨噬细胞抗体anti-macrophage antibody抗巨细胞病毒IgG抗体anti-cytomegalovirus IgG antibody抗巨细胞病毒IgM抗体anti-cytomegalovirus IgM antibody抗狂犬病毒抗体anti-rabies virus antibody抗链球菌溶血素O试验anti-streptolysin O test,ASO test 抗淋巴细胞抗体anti-lymphocyte antibody,ALA抗磷壁酸抗体anti-teichoic acid antibody抗流行性出血热病毒IgG抗体anti-epidemic hemorrhagic fever virus IgG antibody抗流行性出血热病毒IgM抗体anti-epidemic hemorrhagic fever virus IgM antibody抗卵巢抗体anti-ovary antibody抗脑组织抗体anti-brain tissue antibody抗平滑肌抗体anti-smooth muscle antibody抗染色体抗体anti-chromosome antibody抗人类免疫缺陷病毒抗体anti-human immunodeficiency virus antibody抗腮腺管抗体anti-parotid duct antibody抗肾上腺细胞抗体anti-adrenal cell antibody抗肾小球基底膜抗体anti-glomerular basement membrane antibody抗双链DNA抗体anti-double-strand DNA antibody抗胰酶抗体anti-trypsin antibody抗凝血酶Ⅲ抗体anti-trrombine Ⅲ antibody抗生物素蛋白，亲和素avidin抗水痘-带状疱疹病毒抗体anti-varicella-zoster virus antibody抗髓磷脂抗体IgA anti-myelin antibody IgA抗体依赖性细胞介导毒性试验antibody dependent cell-mediated cytotoxin test, ADCC test抗胃壁细胞抗体anti-gastric parietal cell antibody抗戊型肝炎病毒抗体anti-hepatitis E virus antibody抗线粒体抗体anti-mitochondria antibody,AMA抗腺病毒抗体anti-adenovirus antobody抗心肌抗体anti-myocardium antibody抗心磷脂抗体anti-cardiolipin antibody抗血小板相关IgG抗体anti-platelet associated IgG antibody抗胰岛素抗体anti-insulin antibody抗胰岛素受体抗体anti-insulin receptor antibody抗胰岛细胞抗体anti-islet cell antibody抗乙酰胆碱受体抗体anti-acetylcholinergic receptor antibody抗硬皮病抗体anti-scleroderma antibody抗载脂蛋白A1抗体anti-apolipoprotein A1 antibody抗中性粒细胞抗体anti-neutrophil antibody抗中性粒细胞胞质抗体Anti-neutrophil cytoplasmic antibody,ANCA 抗中性粒细胞颗粒抗体anti-neutrophil granules antibody抗着丝点抗体anti-centromere antibody抗组蛋白抗体anti-histone antibody柯萨奇病毒血清学试验Coxsackievirus serological test莱姆病血清学试验Lyme disease serological test类风湿因子rheumatoid factor,RF冷凝集试验cold agglutination test冷球蛋白cryoglobulin立克次体血清学试验rickettsia serological test淋巴因子活化的杀伤细胞lymphokine activated killer cells, LAK cells流行性乙型脑炎病毒血清学试验epidemic encephalitis B virus serological test抗血管内皮细胞抗体anti-endothelial cell antibody,AECA抗血友病因子anti-hemophilic factors抗生物素蛋白(亲和素)-生物素复合物avidin biotin complex,ABC麻疹病毒血清学试验measles virus serological test脉冲场凝胶电泳pulsed-field gel electrophoresis, PFGE酶谱zymogram免疫球蛋白A immunoglobulin A, IgA免疫球蛋白D immunoglobulin D, IgD免疫球蛋白E immunoglobulin E, IgE免疫球蛋白G immunoglobulin G, IgG免疫球蛋白M immunoglobulin M, IgM囊虫病血清学试验cysticercosis serological test尿液轻链试验light chain test in urine农民肺血清学试验farmer's lung serological test疟疾血清学试验malaria serological test球孢子菌病血清学试验coccidioidomycosis serological test曲霉菌病血清学试验aspergillosis serological test人轮状病毒抗原测定human rotavirus antigen test腮腺炎病毒血清学试验mumpus virus serological test沙眼衣原体血清学试验chlamydia trachomatis serological test嗜碱性粒细胞脱颗粒试验basophilic degranulation test嗜异性凝集试验heterophile agglutination test鼠疫血清学试验plague serological test丝虫病血清学试验filariasis serological test土拉菌病血清学试验tularemia serological test外斐试验Weil-Felix reaction外周血α干扰素测定α-interferon determination in peripheralblood戊型肝炎病毒核酸测定hepatitis E virus nucleic acid test限制性片段长度多态性restriction fragment length polymorphism,RFLP新型隐球菌荚膜抗原Cryptococcus neoformans capsular antigen 旋毛虫病血清学试验trichinosis serological test血吸虫病血清学试验schistosomiasis serological test循环免疫复合物测定circulating immunocomplex test恙虫病血清学试验scrub typhus (tsutsugamushi disease)serological test乙型肝炎病毒e抗原hepatitis B virus e antigen, HBeAg乙型肝炎病毒表面抗原hepatitis B virus surface antigen, HBsAg乙型肝炎病毒表面抗原前S2蛋白hepatitis B virus surface antigen pre-s2 protein乙型肝炎病毒核心抗原hepatitis B virus core antigen, HBcAg 支原体肺炎血清学试验mycoplasmal pneumonia serological test 植物血凝素Phytohemagglutinin,PHA自然杀伤细胞功能试验natural killer cell function test自身免疫autoimmunity自体凝集autoagglutination组织胞浆菌血清学试验histoplasma serological test组织中免疫复合物测定immunocomplex test in tissue免疫学补充（邓耀祖）免疫，免疫性immunity免疫学immunology细胞免疫cellular immunity体液免疫humoral immunity抗体antibody,Ab免疫球蛋白immunoglobulin同种型isotype同种异型allotype独特型idiotype重链，H链heavy chain,H chain轻链，L链light chain,L chain可变区variable region高变区hypervariable region单克隆抗体monoclonal antibody,mAb,McAb补体complement细胞因子cytokine干扰素interferon,IFN白细胞介素interleukine, IL集落刺激因子tumor necrosis factor,CSF生长因子growth factor趋化性细胞因子chemokine（遗传）多态性polymorphism连锁不平衡linkage disequilibrumHLA分型HLA typing白细胞分化抗原leukocyte differentiation antigen分化群cluster of differentiation,CD粘附分子adhesion molecule选择素selectin钙粘蛋白cadherin自然杀伤细胞natural killer,NK cell抗原提呈细胞Antigen presenting cell,APC树突状细胞dendritic cell,DCT细胞（抗原）受体T cell receptor辅助性T细胞T help cell，ThB细胞（抗原）受体 B cell receptor,BCR抗原antigen免疫原immunogen半抗原hapton载体carrier抗原决定基Antigenic determinant,Ad表位epitope共同抗原common antigen交叉反应cross-reaction易接近性accessibility胸腺依赖性抗原thymus dependent antigen,TD-Ag 胸腺非依赖性抗原thymus independent antigen,TI-Ag 异种抗原xenogenic antigen同种异体抗原Allogenic antigen自身抗原autoantigen异嗜性抗原heterophilic antigen超抗原superantigen佐剂adjuvant内源性抗原andogenous antigen抑制性T细胞Suppressor T dell,Ts变应原allergen速发型超敏反应Immediate hypersensitivity细胞毒性超敏反应cototoxic hypersensitivity免疫复合物型超敏反应immune-complex hypersensitivity 迟发型超敏反应delayed hypersensitivity自身免疫autoimmunity自身免疫病autoimmune disease免疫缺陷病immunodeficiency disease重症联合免疫缺陷综合征severe combined-immunodeficiency disease,SCID肿瘤抗原tumor antigen(neoantigen)肿瘤特异性抗原tumor specific antigen,TSA 肿瘤相关抗原tumor associated antigen,TAA 胚胎抗原fetal antigen癌胚抗原carcinoembryonic antigen,CEA甲胎蛋白alpha-fetoprotein宿主抗移植物反应host versus graft reaction,HVGR移植物抗宿主反应graft versus host reaction,GVHR组织配型tissue matching凝集agglutination沉淀precipitation免疫扩散immunodiffusion免疫电泳immunoelectrophoresis免疫荧光immunofluorescence酶联免疫吸附试验enzyme linked immunosorbentassay,ELISA生物素-亲和素biotin-avidin放射免疫测定radioimmunoassay,RIA免疫印迹Western blotting免疫治疗immunotherapy主动免疫治疗active immunotherapy被动免疫治疗passive immunotherapy过继免疫治疗adoptive immunotherapy计划免疫planned immunization抗毒素antitoxin抗精子抗体antisperm antibody癌抗原125 cancer antigen 125,CA125癌胚抗原cacinoembryonic antigen,CEA酶免疫测定EIA有需要的相关抗体可以在Fantibody全球抗体搜索引擎进行咨询全球抗体搜索引擎是一个供公共检索的抗体数据库，其抗体信息数据来源于全球范围的研究机构与商业公司。

抗体谱15项

抗体谱15项

抗体谱15项自身免疫性疾病（Autoimmune Disease AID）：免疫系统对自身机体的成分发生免疫反应，造成损害而引起的疾病，可以累积全身过个系统和器官，疾病免疫种类有一百多种，总体发病率在全球占10%左右。

自身抗体（Autoantibody）：抗自身细胞内、细胞表面和细胞外抗原成分的免疫球蛋白。

自身抗体的检测对于自身免疫病的诊断及鉴别、治疗检测和预后评估很重要。

自身抗体谱15项检查主要是用来检查自身免疫性疾病。

如系统性红斑狼疮、干燥综合征、硬皮病、多发性肌炎、原发性胆汁肝硬化、红斑狼疮的活动期以及混合性结缔组织病等。

自身抗体谱15项主要包括抗RNP抗体、抗Sm抗体、抗SSA抗体、抗SSB抗体、抗Ro-52抗体、抗Scl-70抗体、抗Jo-1抗体、抗着丝点抗体、抗核小体抗体、抗总蛋白抗体、抗补体C1q抗体等。

比如系统性红斑狼疮的患者主要有特异性较高的抗体，包括Sm抗体、抗核小体抗体等，而干燥综合征患者检查结果常常会出现SSA抗体、SSB抗体、Ro-52抗体以及60-KD抗体。

所以，不同疾病检查结果可出现不同的抗体。

一．自身免疫性疾病对应抗体谱：1、系统性红斑狼疮：抗U1-nRNP抗体、抗SM抗体、抗SS-A 抗体、抗SS-B抗体、抗PCNA抗体、抗dsDNA抗体、抗核小体抗体、抗核糖体P蛋白抗体、抗线粒体抗体-M2可见于系统性红斑狼疮，抗SS-A抗体、抗Ro-52抗体、抗SS-B抗体也可见于新生儿红斑狼疮，抗组蛋白抗体通常见于药物诱导的红斑狼疮，也可见于非药物诱导的红斑狼疮。

2、硬化症：抗U1-nRNP抗体、抗Ro-52抗体、抗Scl-70抗体、抗PM-Scl抗体、抗着丝点抗体、抗线粒体抗体-M2可见于系统性硬化症，抗SS-A抗体、抗Ro-52抗体、抗着丝点抗体、抗线粒体抗体-M2可见于原发性胆汁性肝硬化症。

3、类风湿性关节炎：抗U1-nRNP抗体、抗组蛋白抗体可见于类风湿性关节炎。

4、干燥综合征：抗SS-A抗体、抗SS-B抗体、抗线粒体抗体-M2可见于干燥综合征。

徐州市无偿献血人群HIV抗体检测结果分析与流调

徐州市无偿献血人群HIV抗体检测结果分析与流调

徐州市无偿献血人群HIV抗体检测结果分析与流调发表时间：2015-10-09T14:00:46.827Z 来源：《医药前沿》2015年第20期供稿作者：祝凯捷孟宪军[导读] 徐州市红十字中心血站近年来随着艾滋病知识广泛宣传普及，特别是对传播途径宣传力度加大。

祝凯捷孟宪军(徐州市红十字中心血站江苏徐州 221006) 【摘要】目的：分析无偿献血者HIV抗体阳性率并进行流调，为严防输血传播HIV提供有效措施。

方法：标本检测采用ELISA法，选择两种不同厂家HIV抗体诊断试剂，有反应性标本送检市CDC艾滋病确认实验室进行确认。

结果：2010～2014年度共检测433204份标本，其中有反应性标本435份，经确认46例均为HIV-1抗体阳性，其阳性率为万分之1.06。

46例病毒感染者男性占44例，40%来至于2次以上自愿的固定的无偿献血者队伍，从学生、职员、工人、农民等职业中均有感染，以性传播为主。

结论：无偿献血者中有HIV感染者，用ELISA法检测存在一定假阳性,需进一步提高诊断试剂性能质量，严格筛查；加强艾滋病感染者流调分析，有针对性的采取干预措施，减少艾滋病输血传播，确保血液安全。

【关键词】无偿献血人群；HIV抗体；流调；ELISA法；WB法【中图分类号】R446 【文献标识码】A 【文章编号】2095-1752（2015）20-0358-03 近年来随着艾滋病知识广泛宣传普及，特别是对传播途径宣传力度加大，人人对艾滋病知识有了较深的认识。

其中通过血液传播如污染针头、输血（指输含有HIV血液）等是传播途径之一，因此每个献血者在献血前要自觉对照献血标准进行自我排除，不符合献血标准的能主动退出，从低危人群中募集血源，现将近五年来无偿献血者HIV抗体检测结果报告如下。

1.材料与方法1.1 标本来源检测标本为我市无偿献血者，2010～2014年度共计433204份，各年度标本数量见表1。

1.2 试剂1.2.1筛查试剂：血站艾滋病筛查实验室选用两种国产试剂，生产厂家分别用A、B表示。

风湿病常见自身抗体

风湿病常见自身抗体
在原发性SS病人中，抗SSA/Ro和抗SSB/La阳性率分别为60% 和40%，它们是诊断干燥综合征的血清学标志。
抗SSB抗体较抗SSA抗体诊断干燥综合征更为特异，敏感性6585%，特异性89%。
抗SSB抗体仅在少数SLE患者中出现，阳性率10%-15%，且大 多为SLE合并SS。
SSA和抗SSB抗体阳性可造成新生儿狼疮及婴儿心脏传导阻滞。 抗SSA和抗SSB抗体常与血管炎、淋巴结肿大、白细胞减少、
偶见于慢性活动性肝炎。 危害：直接参与组织病理损害（特别是皮肤），与光过敏相关，通过胎盘
引起新生儿狼疮综合症（阳性率＞90%），与胎儿传导系统结合，可造成 先天性心脏传导阻滞。 抗SSA两种蛋白（52kD和60kD）均可见于SS、SLE，单独抗Ro-52→SS， 单独抗Ro-60→SLE。
抗SSB抗体
临床上ANA的检测实际上是指总抗核抗体检测。 ANAs分类：抗DNA抗体、抗组蛋白抗体、抗DNA组蛋白复 合物抗体（如抗核小体抗体）、抗非组蛋白抗体（如抗ENA 抗体、抗着丝点抗体）、抗核仁抗体、抗其他细胞成分抗体
ANA+ANA谱
• ANA 1.高滴度ANA阳性提示风湿免疫病可能 2.非结缔组织病也可呈阳性：
抗U1RNP抗体在混合性结缔组织病（MCTD）中几乎均为阳性（阳性 率＞95%），且其滴度很高。
出现高效价抗U1RNP抗体，且无其他特异性抗核抗体，是诊断MCTD 的重要血清学证据。
抗U1RNP抗体阳性的病人，常与肌炎、食管蠕动功能低下、雷诺现象、 关节痛、指（趾）端硬化和肺间质性改变等症状相关，且肾炎发病率低。
MPO-ANCA与原发性血管炎相关性不及PR3-ANCA与WG相 关性那样紧密。但每一个怀疑血管炎或肾小球肾炎患者，只要 原因不明，就应查ANCA。

免疫组化抗体表

免疫组化抗体表
胞膜
33
CD30 (=Ki-1 Antigen)
Ber-H2
标记大细胞间变性淋巴瘤，何杰金病R-S细胞，活化的T、B细胞。生殖细胞性肿瘤，如胚胎性癌（+）。
胞膜/胞浆
34
CD31
JC70A
属免疫球蛋白超家族，在所有连续性内皮表达，但不连续的血窦内皮不表达。巨核细胞、血小板、髓细胞、NK细胞和部分T、B细胞也有表达。
胞浆
10
Androgen Receptor（AR）
雄激素受体
AR411
前列腺癌预后治疗指标
胞核
11
BCL-10
151
具有t（1；14）（p22；q32）染色体异位的黏膜相关淋巴瘤中强表达，提示预后差
核/浆
12
Bcl-2
124
用于滤泡型淋巴瘤与反应性增生的滤泡间鉴别。前者肿瘤性滤泡中心及周围细胞均阳性，后者仅滤泡周围细胞阳性。神经内分泌细胞、1/3的孤立性纤维性肿瘤（+）。
胞膜/胞浆
13
BCL-6
PG-B6P
标记正常生发中心B细胞及其来源肿瘤。弥漫性大B细胞淋巴瘤（+）。B-CLL、毛细胞性白血病、套细胞及边缘区细胞淋巴瘤（-）。
胞核
英文名称及缩写
中文名称
克隆号
主要特点及用途
表达部位
14
β-catenin
WNT信号通路的重要调节蛋白。在静止细胞中与E-Cadherin的胞内部分结合，以稳定细胞间粘附；在WNT信号激活的细胞中由胞浆进入胞核成为转录激活因子。其自身发生突变或与之结合的APC蛋白异常时，可出现异常核表达，见于80%的大肠癌、胰腺实性假乳头性肿瘤、深部软组织（韧带样型）纤维瘤病、鼻腔血管纤维瘤。

产品技术手册

产品技术手册

表1 检测TSH抗体配对建议
5401
5403*
捕 5404
获 抗
5405
体 5407
5408
5409*
5401 – + – – – – –
5403* + – + + + + –
5404 – + – – – – –
检测抗体 5405 + + + – – + +
5407 + + + – – – +
* 识别TSHα亚基和β亚基结合处的抗原表位；
"Hormones are signaling molecules
produced by the endocrine glands."
激素是一组不同的信号分子，主要由内分泌腺产生。从 生殖、性别分化、生长发育到代谢调控和细胞平衡的保 持，它们控制着这些基础过程中的方方面面。由于激素 控制着大量不同的细胞及机体过程，因此很多失调与内 分泌功能障碍有关。如某些癌症如甲状腺、睾丸及卵巢 肿瘤是内分泌腺依赖性的，通过内分泌腺产生过多的激 素刺激其自身的生长。
克隆号 5404 5405 5409
产品编号 600020 600021 600023
质保期 (月，2-8°C)
12 36 36 36 24 36 36
亚型
IgG 1
IgG 1
IgG 1
IgG 2a
IgG 1
IgG 1
IgG 1
应用
ELISA ELISA ELISA ELISA ELISA ELISA ELISA


391
*)
游离的β亚基特异性抗体；

实验室检测常用技术

实验室检测常用技术

试管法
结果判定 5%石碳酸的10%盐溶液
注：第11孔为阳性对照，第12孔为阴性对照。 37℃～38℃水浴20min
参照比浊管，按各试管上层液体清亮度判读 5%-1%的红细胞悬液
实时荧光定量PCR技术于1996年由美国Applied Biosytems公司推出，该技术实现了PCR从定性到定量的飞跃。
抗原、阳性血清和阴性血清 凝集试验管（三分管）、试管架、吸管及温箱
试管法
置37℃～40 ℃温箱24h，取出检查并记录结果。
试管法
每次试验均应设阳性血清、阴性血清和抗原对照。
牛、马、鹿、骆驼血清稀释法与上述基本一致，差异 是第一管加稀释液和被检血清。
大规模检疫时也可只用2个稀释度，即牛、马、鹿、 骆驼用1:50和1:100，猪、山羊、绵羊和狗用1:25和1:50 。
ELISA可用于检测抗体，也可用于检测抗原。
实验的方法类型有多种，如双抗体夹心法、间接法、竞争 法和捕获法等，虽然原理不尽相同，但实验特点和影响因 素基本一样。
酶
抗抗 体
酶标记 抗抗体
抗体
1.将抗原包被在固相载体上。 2.如样品中含有抗体，则结合
为抗原抗体复合物。 3.再加入酶标记抗抗体，则结合 为抗原-抗体-酶标记抗抗体复合物。
一定时间，形成肉眼可见的凝集团块，称为凝集试验（agglutination）。 1985年美国的Perkin-Elmer Cetus 公司的发明了具有划时代意义的聚合酶链反应（PCR）。 振荡混匀，于室温（20℃-25 ℃ ）放置40分钟后判定结果（如果环境温度太高，可置4℃环境下）。
试管法
结果判定
凝集试验可用于检测抗原或抗体，最突出的优点是操 作简便。
凝集试验可根据抗原的性质、反应的方式分为直接凝 集试验（简称凝集试验）和间接凝集试验。
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产品货号产品详细名称反应种属PMK001S Flag-Tag Mouse Monoclonal Antibody(2C5)N/A PMK001M Flag-Tag Mouse Monoclonal Antibody(2C5)N/A PMK002S C-Myc-Tag Mouse Monoclonal Antibody(3E8)N/A PMK002M C-Myc-Tag Mouse Monoclonal Antibody(3E8)N/A PMK003S HA-Tag Mouse Monoclonal Antibody(1B10)N/A PMK003M HA-Tag Mouse Monoclonal Antibody(1B10)N/A PMK004S His-Tag Mouse Monoclonal Antibody(4E6)N/A PMK004M His-Tag Mouse Monoclonal Antibody(4E6)N/A PMK005S V5-Tag Mouse Monoclonal Antibody(3C8)N/A PMK005M V5-Tag Mouse Monoclonal Antibody(3C8)N/A PMK006S VSV-G-Tag Mouse Monoclonal Antibody(8D6)N/A PMK006M VSV-G-Tag Mouse Monoclonal Antibody(8D6)N/A PMK007S GST-Tag Mouse Monoclonal Antibody(6D2)N/A PMK007M GST-Tag Mouse Monoclonal Antibody(6D2)N/A PMK008S MBP-Tag Mouse Monoclonal Antibody(7G2)N/A PMK008M MBP-Tag Mouse Monoclonal Antibody(7G2)N/A PMK009S GFP-Tag Mouse Monoclonal Antibody(1G6)N/A PMK009M GFP-Tag Mouse Monoclonal Antibody(1G6)N/A PMK010S mCherry-Tag Mouse Monoclonal Antibody(6B3)N/A PMK010M mCherry-Tag Mouse Monoclonal Antibody(6B3)N/A PMK011S RFP-Tag Mouse Monoclonal Antibody(5G4)N/A PMK011M RFP-Tag Mouse Monoclonal Antibody(5G4)N/A PMK012S T7-Tag Mouse Monoclonal Antibody(1H5)N/A PMK012M T7-Tag Mouse Monoclonal Antibody(1H5)N/A PMK013S E2-Tag Mouse Monoclonal Antibody(4F8)N/A PMK013M E2-Tag Mouse Monoclonal Antibody(4F8)N/A PMK014S HSV-Tag Mouse Monoclonal Antibody(9D7)N/A PMK014M HSV-Tag Mouse Monoclonal Antibody(9D7)N/A PMK015S KT3-Tag Mouse Monoclonal Antibody(3D11)N/A PMK015M KT3-Tag Mouse Monoclonal Antibody(3D11)N/A PMK016S Strep-Tag Mouse Monoclonal Antibody(7A10)N/A PMK016M Strep-Tag Mouse Monoclonal Antibody(7A10)N/A PMK017S S-Tag Mouse Monoclonal Antibody(3B3)N/A PMK017M S-Tag Mouse Monoclonal Antibody(3B3)N/A PMK018S Trx-Tag Mouse Monoclonal Antibody(5H9)N/A PMK018M Trx-Tag Mouse Monoclonal Antibody(5H9)N/A PMK019S GFP-Tag Mouse Monoclonal Antibody(2G6)N/A PMK019M GFP-Tag Mouse Monoclonal Antibody(2G6)N/A PMK020S GST Mouse Monoclonal Antibody （Q9）N/A PMK020M GST Mouse Monoclonal Antibody （Q9）N/A PMK030S GST Mouse Monoclonal Antibody （Q16）N/A PMK030M GST Mouse Monoclonal Antibody （Q16）N/A PMK031S V5-Tag Mouse Monoclonal Antibody(Q31)N/A PMK031M V5-Tag Mouse Monoclonal Antibody(Q31)N/A PMK032S His-Tag Mouse Monoclonal Antibody(4E6)-HRP conjuga N/A PMK032M His-Tag Mouse Monoclonal Antibody(4E6)-HRP conjuga N/A PMK033S C-Myc-Tag Mouse Monoclonal Antibody(5D6)N/A PMK033M C-Myc-Tag Mouse Monoclonal Antibody(5D6)N/A PMK034S Flag-Tag Mouse Monoclonal Antibody(2C5)-HRP conjug N/A PMK034M Flag-Tag Mouse Monoclonal Antibody(2C5)-HRP conjug N/A PMK035S HA-Tag Mouse Monoclonal Antibody(1B10)-HRP conjuga N/APMK036M C-Myc-Tag Mouse Monoclonal Antibody(3E8)-HRP conju N/APMK021S Flag-Tag Rabbit Polyclonal Antibody N/APMK021M Flag-Tag Rabbit Polyclonal Antibody N/APMK022S HA-Tag Rabbit Polyclonal Antibody N/APMK022M HA-Tag Rabbit Polyclonal Antibody N/APMK023S C-Myc-Tag Rabbit Polyclonal Antibody N/APMK023M C-Myc-Tag Rabbit Polyclonal Antibody N/APMK024S His-Tag Rabbit Polyclonal Antibody N/APMK024M His-Tag Rabbit Polyclonal Antibody N/APMK025S GST-Tag Rabbit Polyclonal Antibody N/APMK025M GST-Tag Rabbit Polyclonal Antibody N/APMK026S GFP-Tag Rabbit Polyclonal Antibody N/APMK026M GFP-Tag Rabbit Polyclonal Antibody N/APMK027S mCherry-Tag Rabbit Polyclonal Antibody N/APMK027M mCherry-Tag Rabbit Polyclonal Antibody N/APMK028S RFP-Tag Rabbit Polyclonal Antibody N/APMK028M RFP-Tag Rabbit Polyclonal Antibody N/APMK029S MBP-Tag Rabbit Polyclonal Antibody N/APMK029M MBP-Tag Rabbit Polyclonal Antibody N/APMK037S RFP-Tag Mouse Polyclonal Antibody N/APMK037M RFP-Tag Mouse Polyclonal Antibody N/APMK038S GFP-Tag Mouse Polyclonal Antibody N/APMK038M GFP-Tag Mouse Polyclonal Antibody N/APMK039S mCherry-Tag Mouse Polyclonal Antibody N/APMK039M mCherry-Tag Mouse Polyclonal Antibody N/APMK040S EYFP-Tag Mouse Polyclonal Antibody N/APMK040M EYFP-Tag Mouse Polyclonal Antibody N/AS013M Normal Mouse IgGS014M Normal Rabbit IgGPMK041Sβ-actin Mouse Monoclonal Antibody(5B7)H, R, M, Mk, Dg, Ch PMK041Mβ-actin Mouse Monoclonal Antibody(5B7)H, R, M, Mk, Dg, Ch PMK042S GAPDH Mouse Monoclonal Antibody(2B8)H, R, M, Mk, Dg, C, PMK042M GAPDH Mouse Monoclonal Antibody(2B8)H, R, M, Mk, Dg, C, PMK043Sβ-Tubulin Mouse Monoclonal Antibody(5G3)H, R, M, Mk, Dg, Ch PMK043Mβ-Tubulin Mouse Monoclonal Antibody(5G3)H, R, M, Mk, Dg, Ch PMK044S PCNA Mouse Monoclonal Antibody(12D10)H, R, MPMK044M PCNA Mouse Monoclonal Antibody(12D11)H, R, MPMK045Sβ-actin Mouse Monoclonal Antibody(A02)H, R, M, Mk, Dg, Ch PMK045Mβ-actin Mouse Monoclonal Antibody(A02)H, R, M, Mk, Dg, Ch PMK046S COX IV Mouse Monoclonal Antibody(TDY100A-6C8)H, R, MPMK046M COX IV Mouse Monoclonal Antibody(TDY100A-6C8)H, R, MPMK047S Plant actin Mouse Monoclonal Antibody(Q30)Various Plants PMK047M Plant actin Mouse Monoclonal Antibody(Q30)Various Plants PMK048Sα-tubulin Mouse Monoclonal Antibody（8F11）H, R, MPMK048Mα-tubulin Mouse Monoclonal Antibody（8F11）H, R, MPMK049S Lamin B1 Mouse Monoclonal Antibody(7C11)H, R, MPMK049M Lamin B1 Mouse Monoclonal Antibody(7C11)H, R, MPMK050Sβ I tubulin Mouse Monoclonal Antibody(3F7)H, R, MPMK050Mβ I tubulin Mouse Monoclonal Antibody(3F7)H, R, MPMK051Sβ-actin Rabbit Polyclonal Antibody H、M、R、Rb、Ch 、Mk、Sh、XenopusPMK051Mβ-actin Rabbit Polyclonal Antibody H、M、R、Rb、Ch 、Mk、Sh、XenopusPMK052S GAPDH Rabbit Polyclonal Antibody H、M、R、Rb、Ch 、Mk、Sh、XenopusPMK052M GAPDH Rabbit Polyclonal Antibody H、M、R、Rb、Ch 、Mk、Sh、XenopusPMK053S Plant-actin Rabbit Polyclonal Antibody Arabidopsis Nicotiana tabacum Oryza sativa Hordeum vulgarePMK053M Plant-actin Rabbit Polyclonal Antibody Arabidopsis Nicotiana tabacum Oryza sativa Hordeum vulgarePMK054Sα-tubulin Rabbit Polyclonal Antibody H、M、RPMK054Mα-tubulin Rabbit Polyclonal Antibody H、M、RPMK055S Histone H3 Mouse Monoclonal Antibody(1G1)H、M、R、Yeast PMK055M Histone H3 Mouse Monoclonal Antibody(1G1)H、M、R、Yeast PMK056Sβ-actin Mouse Monoclonal Antibody(5B7)-HRP conjugatedH, R, M, Mk, Dg, Ch PMK056Mβ-actin Mouse Monoclonal Antibody(5B7)-HRP conjugatedH, R, M, Mk, Dg, Ch PMK057S GAPDH Mouse Monoclonal Antibody(2B8)-HRP conjugate H, R, M, Mk, Dg, C, PMK057M GAPDH Mouse Monoclonal Antibody(2B8)-HRP conjugate H, R, M, Mk, Dg, C, PMK058S ECFP-Tag Mouse Polyclonal Antibody N/APMK058M ECFP-Tag Mouse Polyclonal Antibody N/APMK059S Digoxin Mouse Monoclonal Antibody(4F6)N/APMK059M Digoxin Mouse Monoclonal Antibody(4F6)N/APMK060S Plant GST Rabbbit Polyclonal Antibody Nicotianatabacum PMK060M Plant GST Rabbbit Polyclonal Antibody Nicotianatabacum PMK061S Bcl-2 Mouse Monoclonal Antibody（6B5）H,M,RPMK061M Bcl-2 Mouse Monoclonal Antibody（6B5）H,M,RPMK062S HSP70 Mouse Monoclonal Antibody (3G10)H,M,RPMK062M HSP70 Mouse Monoclonal Antibody (3G10)H,M,RPMK063S Galectin-3 Mouse Monoclonal Antibody (6G2)HPMK063M Galectin-3 Mouse Monoclonal Antibody (6G2)HPMK064S EGFR Mouse Monoclonal Antibody (1B1)HPMK064M EGFR Mouse Monoclonal Antibody (1B1)HPMK065S ErbB-2/HER-2 Mouse Monoclonal Antibody (11H9)H,M,RPMK065M ErbB-2/HER-2 Mouse Monoclonal Antibody (11H9)H,M,RPMK066S CD45 Mouse Monoclonal Antibody (12A9)HPMK066M CD45 Mouse Monoclonal Antibody (12A9)HPMK067S AMACR/P504S Mouse Monoclonal Antibody (4A12)H,M,RPMK067M AMACR/P504S Mouse Monoclonal Antibody (4A12)H,M,RPMK068S MeCP2 Rabbit Polyclonal Antibody HPMK068M MeCP2 Rabbit Polyclonal Antibody HPMK069S EFHD1 Rabbit Polyclonal Antibody HPMK069M EFHD1 Rabbit Polyclonal Antibody HPMK070S FH/Fumarase Rabbit Polyclonal Antibody H,M,RPMK070M FH/Fumarase Rabbit Polyclonal Antibody H,M,RPMK071S CD20 Mouse Monoclonal Antibody (2F4)H,M,RPMK072M CD23 Mouse Monoclonal Antibody (1E9)H,M,RPMK073S CD68 Mouse Monoclonal Antibody (6F3)H,M,RPMK073M CD68 Mouse Monoclonal Antibody (6F3)H,M,RPMK074S CK19 Mouse Monoclonal Antibody (11F5)H,M,RPMK074M CK19 Mouse Monoclonal Antibody (11F5)H,M,RPMK075S p53 Mouse Monoclonal Antibody (6C4)HPMK075M p53 Mouse Monoclonal Antibody (6C4)HPMK076S NFkB p65 Mouse Monoclonal Antibody (14H2)H,M,RPMK076M NFkB p65 Mouse Monoclonal Antibody (14H2)H,M,RPMK077S CK7 Mouse Monoclonal Antibody (12D7)H,M,RPMK077M CK7 Mouse Monoclonal Antibody (12D7)H,M,RPMK078S CK8 Mouse Monoclonal Antibody (8G8)H,M,RPMK078M CK8 Mouse Monoclonal Antibody (8G8)H,M,RPMK079S CK16 Mouse Monoclonal Antibody (6F6)H,M,RPMK079M CK16 Mouse Monoclonal Antibody (6F6)H,M,RPMK080S CDX2 Mouse Monoclonal Antibody（14H6）H,M,RPMK080M CDX2 Mouse Monoclonal Antibody（14H6）H,M,RPMK081S Myosin Heavy Chain Mouse Monoclonal Antibody (11C2H,M,R,Fruit Fly,Nem PMK081M Myosin Heavy Chain Mouse Monoclonal Antibody (11C2H,M,R,Fruit Fly,Nem PMK082S GFAP Mouse Monoclonal Antibody (5C8)R，MPMK082M GFAP Mouse Monoclonal Antibody (5C8)R，MPMK083S CD21 Mouse Monoclonal Antibody（2C5）H,M,RPMK083M CD21 Mouse Monoclonal Antibody（2C5）H,M,RPMK084S Carcinoembryonic Antigen/CEA Mouse Monoclonal Anti HPMK084M Carcinoembryonic Antigen/CEA Mouse Monoclonal Anti HPMK085S Aquaporin 4 Mouse Monoclonal Antibody(4H1)H,M,RPMK085M Aquaporin 4 Mouse Monoclonal Antibody(4H1)H,M,RPMK086S Kif 7 Mouse Monoclonal Antibody(3F8)H,M,RPMK086M Kif 7 Mouse Monoclonal Antibody(3F8)H,M,RPMK087S Ki 67 Mouse Monoclonal Antibody(4A8)H,M,RPMK087M Ki 67 Mouse Monoclonal Antibody(4A8)H,M,RPMK088Sβ-Catenin Mouse Monoclonal Antibody(13C6)H,M,RPMK088Mβ-Catenin Mouse Monoclonal Antibody(13C6)H,M,RPMK089S NSE Mouse Monoclonal Antibody(13E2)H,M,RPMK089M NSE Mouse Monoclonal Antibody(13E2)H,M,RPMK090S MAP2 Mouse Monoclonal Antibody(7D4)H,M,RPMK090M MAP2 Mouse Monoclonal Antibody(7D4)H,M,RPMK091S COX1/Cyclooxygenase 1 Rabbit Polyclonal AntibodyHPMK091M COX1/Cyclooxygenase 1 Rabbit Polyclonal AntibodyHPMK092S COX2/Cyclooxygenase 2 Rabbit Polyclonal AntibodyHPMK092M COX2/Cyclooxygenase 2 Rabbit Polyclonal AntibodyHPMK093S CD1 Mouse Monoclonal Antibody(9H6)H,M,RPMK093M CD1 Mouse Monoclonal Antibody(9H6)H,M,RPMK094S CD2 Mouse Monoclonal Antibody(3F3)H,M,RPMK094M CD2 Mouse Monoclonal Antibody(3F3)H,M,RPMK095S CD4 Mouse Monoclonal Antibody(11A1)H,M,RPMK095M CD4 Mouse Monoclonal Antibody(11A1)H,M,RPMK096S CD5 Mouse Monoclonal Antibody(10G8)H,M,RPMK096M CD5 Mouse Monoclonal Antibody(10G8)H,M,RPMK097S CD10 Mouse Monoclonal Antibody(5B8)H,M,RPMK097M CD10 Mouse Monoclonal Antibody(5B8)H,M,RPMK098S FH/Fumarase Mouse Monoclonal Antibody(7F1)H,M,RPMK099M ABCB5 Mouse Monoclonal Antibody(11A2)HPMK100S AFP/alpha 1 Fetoprotein Mouse Monoclonal Antibody(HPMK100M AFP/alpha 1 Fetoprotein Mouse Monoclonal Antibody(HPMK101S CA IX/Carbonic Anhydrase IX Mouse Monoclonal Antib HPMK101M CA IX/Carbonic Anhydrase IX Mouse Monoclonal Antib HPMK102S Caspase 9 Mouse Monoclonal Antibody(PMK3-20)H,M,RPMK102M Caspase 9 Mouse Monoclonal Antibody(PMK3-20)H,M,RPMK103S ERCC1 Mouse Monoclonal Antibody(4F12)HPMK103M ERCC1 Mouse Monoclonal Antibody(4F12)HPMK104S ErbB-2/HER-2 Mouse Monoclonal Antibody (3C9)HPMK104M ErbB-2/HER-2 Mouse Monoclonal Antibody (3C9)HPMK105S Cytokeratin 6 Mouse Monoclonal Antibody(4A1)HPMK105M Cytokeratin 6 Mouse Monoclonal Antibody(4A1)HPMK106S CK17 Mouse Monoclonal Antibody(10A1)HPMK106M CK17 Mouse Monoclonal Antibody(10A1)HPMK107S CD23 Mouse Monoclonal Antibody (10H2)H,M,RPMK107M CD23 Mouse Monoclonal Antibody (10H2)H,M,RPMK108S IDE/Insulin Degrading Enzyme Mouse Monoclonal Anti HPMK108M IDE/Insulin Degrading Enzyme Mouse Monoclonal Anti HPMK109S alpha Lactalbumin Mouse Monoclonal Antibody(9E9)HPMK109M alpha Lactalbumin Mouse Monoclonal Antibody(9E9)HPMK110S Alliinase Rabbit Polyclonal Antibody Allium sativum L PMK110M Alliinase Rabbit Polyclonal Antibody Allium sativum L PMK111S EFHD1 Mouse Monoclonal Antibody(3G2)H,M,RPMK111M EFHD1 Mouse Monoclonal Antibody(3G2)H,M,RPMK112S XRCC4 Mouse Monoclonal Antibody(5C10)HPMK112M XRCC4 Mouse Monoclonal Antibody(5C10)HPMK113S Glucocorticoid receptor Rabbit Polyclonal Antibody H,M,RPMK113M Glucocorticoid receptor Rabbit Polyclonal Antibody H,M,RPMK114S Histone H3 (di methyl K79) Mouse Monoclonal Antibo H,M,RPMK114M Histone H3 (di methyl K79) Mouse Monoclonal Antibo H,M,RPMK115S Histone H3 (tri methyl K36) Mouse Monoclonal Antib H,M,RPMK115M Histone H3 (tri methyl K36) Mouse Monoclonal Antib H,M,RPMK116S Oct1 Mouse Monoclonal Antibody(TDY27-7G1)HPMK116M Oct1 Mouse Monoclonal Antibody(TDY27-7G1)HPMK117S CD16 Mouse Monoclonal Antibody(Q32)HPMK117M CD16 Mouse Monoclonal Antibody(Q32)HPMK118S Histone H3 (tri methyl K79) Mouse Monoclonal Antib H,M,RPMK118M Histone H3 (tri methyl K79) Mouse Monoclonal Antib H,M,RPMK119S Histone H3 (tri methyl K79) Mouse Monoclonal Antib H,M,RPMK119M Histone H3 (tri methyl K79) Mouse Monoclonal Antib H,M,RPMK120S Histone H3 (mono methyl K79) Mouse Monoclonal Anti H,M,RPMK120M Histone H3 (mono methyl K79) Mouse Monoclonal Anti H,M,RPMK121S Histone H3 (tri methyl K79) Mouse Monoclonal Antib H,M,RPMK121M Histone H3 (tri methyl K79) Mouse Monoclonal Antib H,M,RPMK122S Cytokeratin 18 Mouse Monoclonal Antibody(1B3)H,M,RPMK122M Cytokeratin 18 Mouse Monoclonal Antibody(1B3)H,M,RPMK123S Cytokeratin 18 Mouse Monoclonal Antibody(2F7)H,MPMK123M Cytokeratin 18 Mouse Monoclonal Antibody(2F7)H,MPMK124S Cytokeratin 18 Mouse Monoclonal Antibody(8F2)H,M,RPMK124M Cytokeratin 18 Mouse Monoclonal Antibody(8F2)H,M,RPMK125S Histone H3(mono methyl K79) Mouse Monoclonal Antib H,M,RPMK126M Histone H3(mono methyl K79) Mouse Monoclonal Antib H,M,R PMK127S Histone H3(tri methyl K9) Mouse Monoclonal Antibod H,M,R PMK127M Histone H3(tri methyl K9) Mouse Monoclonal Antibod H,M,R PMK128S Histone H3(di methyl K27) Mouse Monoclonal Antibod H,M,R PMK128M Histone H3(di methyl K27) Mouse Monoclonal Antibod H,M,R PMK129S Histone H3(di methyl K27) Mouse Monoclonal Antibod H,M,R PMK129M Histone H3(di methyl K27) Mouse Monoclonal Antibod H,M,R PMK130S Histone H3(mono methyl K79) Mouse Monoclonal Antib H,M,R PMK130M Histone H3(mono methyl K79) Mouse Monoclonal Antib H,M,R PMK131S Histone H3(tri methyl K4) Mouse Monoclonal Antibod H,M,R PMK131M Histone H3(tri methyl K4) Mouse Monoclonal Antibod H,M,R PMK132S CD15 Mouse Monoclonal Antibody(Q89)HPMK132M CD15 Mouse Monoclonal Antibody(Q89)HPMK133S CD41/Integrin α2b Mouse Monoclonal Antibody(Q90)HPMK133M CD41/Integrin α2b Mouse Monoclonal Antibody(Q90)HPMK134S CD25 Mouse Monoclonal Antibody(Q22)HPMK134M CD25 Mouse Monoclonal Antibody(Q22)HPMK135S Histone H3(di methyl K9) Mouse Monoclonal Antibody H,M,R PMK135M Histone H3(di methyl K9) Mouse Monoclonal Antibody H,M,R PMK136S Histone H3(tri methyl K9) Mouse Monoclonal Antibod H,M,R PMK136M Histone H3(tri methyl K9) Mouse Monoclonal Antibod H,M,R PMK137S Histone H3(mono methyl K9) Mouse Monoclonal Antibo H,M,R PMK137M Histone H3(mono methyl K9) Mouse Monoclonal Antibo H,M,R PMK138S NFkB p65 Mouse Monoclonal Antibody (5G6)H,M,R PMK138M NFkB p65 Mouse Monoclonal Antibody (5G6)H,M,R PMK139S Peroxiredoxin 1 Mouse Monoclonal Antibody (8E7)H,M,R PMK139M Peroxiredoxin 1 Mouse Monoclonal Antibody (8E7)H,M,R PMK140S HSV-Tag Mouse Monoclonal Antibody(Q72)N/APMK140M HSV-Tag Mouse Monoclonal Antibody(Q72)N/APMK141S GAPDH Rabbit Polyclonal Antibody（Zebrafish Specif Zebrafish PMK141M GAPDH Rabbit Polyclonal Antibody（Zebrafish Specif ZebrafishZebrafish PMK142Sβ-Tubulin Mouse Monoclonal Antibody（Zebrafish Specific）Zebrafish PMK142Mβ-Tubulin Mouse Monoclonal Antibody（Zebrafish Specific）Zebrafish PMK143Sα-tubulin Mouse Monoclonal Antibody（Zebrafish Specific）Zebrafish PMK143Mα-tubulin Mouse Monoclonal Antibody（Zebrafish Specific）PMK144S Insulin Glargine Mouse Monoclonal Antibody(3F12)N/APMK144M Insulin Glargine Mouse Monoclonal Antibody(3F12)N/APMK145S Histone H3(di methyl K79) Mouse Monoclonal Antibod H,M,R PMK145M Histone H3(di methyl K79) Mouse Monoclonal Antibod H,M,R PMK146S Histone H3(di methyl K36) Mouse Monoclonal Antibod H,M,R PMK146M Histone H3(di methyl K36) Mouse Monoclonal Antibod H,M,R PMK147Sβ I tubulin Mouse Monoclonal Antibody(Zebrafish Specific)ZebrafishZebrafish PMK147Mβ I tubulin Mouse Monoclonal Antibody(Zebrafish Specific) PMK148S Histone H3 Mouse Monoclonal Antibody(Zebrafish Spe Zebrafish PMK148M Histone H3 Mouse Monoclonal Antibody(Zebrafish Spe ZebrafishZebrafish PMK149Sβ-actin Mouse Monoclonal Antibody (Zebrafish Specific)Zebrafish PMK149Mβ-actin Mouse Monoclonal Antibody (Zebrafish Specific) PMK150S Plant actin Mouse Monoclonal Antibody(2E3)Plants PMK150M Plant actin Mouse Monoclonal Antibody(2E3)Plants PMK151S Collagen III Mouse Monoclonal Antibody(Q76)H,M,R PMK151M Collagen III Mouse Monoclonal Antibody(Q76)H,M,R PMK152S GFP Mouse Monoclonal Antibody(Mix-mA™)N/APMK153M mCherry Mouse Monoclonal Antibody(Mix-mA™)N/APMK154S CBP Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK154M CBP Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK155S TAP Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK155M TAP Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK156S GFP Mouse Monoclonal Antibody(Plant Specific)N/APMK156M GFP Mouse Monoclonal Antibody(Plant Specific)N/APMK157S His-Tag Mouse Monoclonal Antibody(M1)N/APMK157M His-Tag Mouse Monoclonal Antibody(M1)N/APMK158S HSP90β Mouse Monoclonal Antibody(M2)H,M,RPMK158M HSP90β Mouse Monoclonal Antibody(M2)H,M,RPMK159S PARP Mouse Monoclonal Antibody(M3)HPMK159M PARP Mouse Monoclonal Antibody(M3)HPMK160S Cleaved PARP Mouse Monoclonal Antibody(M4)HPMK160M Cleaved PARP Mouse Monoclonal Antibody(M4)HPMK161Sβ-actin Mouse Monoclonal Antibody(M5)H, R, M, Mk, Dg PMK161Mβ-actin Mouse Monoclonal Antibody(M5)H, R, M, Mk, Dg PMK162S EGFR Mouse Monoclonal Antibody(M6)HPMK162M EGFR Mouse Monoclonal Antibody(M6)HPMK163Sβ-tubulin Mouse Monoclonal Antibody(M7)H,M,RPMK163Mβ-tubulin Mouse Monoclonal Antibody(M7)H,M,RPMK164S eIF4A1 Mouse Monoclonal Antibody(M8)H,M,RPMK164M eIF4A1 Mouse Monoclonal Antibody(M8)H,M,RPMK165S Fibronectin Mouse Monoclonal Antibody(M9)H,M,RPMK165M Fibronectin Mouse Monoclonal Antibody(M9)H,M,RPMK166Sβ-actin Mouse Monoclonal Antibody(Mix-mA™)H, R, M, Mk, Dg, Ch PMK166Mβ-actin Mouse Monoclonal Antibody(Mix-mA™)H, R, M, Mk, Dg, Ch PMK167Sβ-tubulin Mouse Monoclonal Antibody(Mix-mA™)H, R, M, Mk, Dg, Ch PMK167Mβ-tubulin Mouse Monoclonal Antibody(Mix-mA™)H, R, M, Mk, Dg, Ch PMK168S HA-Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK168M HA-Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK169S His-Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK169M His-Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK170S c-Myc-Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK170M c-Myc-Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK171S V5-Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK171M V5-Tag Mouse Monoclonal Antibody(Mix-mA™)N/APMK172S GST Mouse Monoclonal Antibody(Mix-mA™)N/APMK172M GST Mouse Monoclonal Antibody(Mix-mA™)N/APMK173S Cleaved PARP Mouse Monoclonal Antibody(Mix-mA™)HPMK173M Cleaved PARP Mouse Monoclonal Antibody(Mix-mA™)HPMK174S ECFP Rabbit Polyclonal Antibody N/APMK174M ECFP Rabbit Polyclonal Antibody N/APMK175S EYFP Rabbit Polyclonal Antibody N/APMK175M EYFP Rabbit Polyclonal Antibody N/APMK176S mOrange Rabbit Polyclonal Antibody N/APMK176M mOrange Rabbit Polyclonal Antibody N/APMK177S Rubisco Rabbit Polyclonal Antibody无货PMK177M Rubisco Rabbit Polyclonal Antibody无货PMK178S AmCyan Mouse Polyclonal Antibody N/APMK178M AmCyan Mouse Polyclonal Antibody N/APMK179S BFP Mouse Polyclonal Antibody N/APMK180M mStrawberry Mouse Polyclonal Antibody N/APMK181S mStrawberry Rabbit Polyclonal Antibody N/APMK181M mStrawberry Rabbit Polyclonal Antibody N/APMK182S Rubisco Mouse Polyclonal Antibody Various Plants PMK182M Rubisco Mouse Polyclonal Antibody Various Plants PMK183S mOrange Mouse Polyclonal Antibody N/APMK183M mOrange Mouse Polyclonal Antibody N/APMK184S AmCyan Rabbit Polyclonal Antibody N/APMK184M AmCyan Rabbit Polyclonal Antibody N/APMK185S BFP Rabbit Polyclonal Antibody N/APMK185M BFP Rabbit Polyclonal Antibody N/APMK186S HA-Tag Mouse Monoclonal Antibody(2C1)N/APMK186M HA-Tag Mouse Monoclonal Antibody(2C1)N/APMK187S Plant actin Mouse Monoclonal Antibody(Mix-mA™)Various Plants PMK187M Plant actin Mouse Monoclonal Antibody(Mix-mA™)Various Plants PMK188S Histone H3 Mouse Monoclonal Antibody(Mix-mA™)H,M,RPMK188M Histone H3 Mouse Monoclonal Antibody(Mix-mA™)H,M,RH,M,RPMK189Sα skeletal muscle actin Mouse Monoclonal Antibody(4B11)H,M,RPMK189Mα skeletal muscle actin Mouse Monoclonal Antibody(4B11)PMK190S Histone H3 Mouse Monoclonal Antibody(8F7)H,M,RPMK190M Histone H3 Mouse Monoclonal Antibody(8F7)H,M,RM,R,(H)PMK191Sβ II tubulin Mouse Monoclonal Antibody(Mix-mA™) (Rodent Preferred)M,R,(H)PMK191Mβ II tubulin Mouse Monoclonal Antibody(Mix-mA™) (Rodent Preferred) PMK192S Transferrin Mouse Monoclonal Antibody(7F4)HPMK192M Transferrin Mouse Monoclonal Antibody(7F4)HPMK193S Avi-Tag Mouse Monoclonal Antibody(6E3)N/APMK193M Avi-Tag Mouse Monoclonal Antibody(6E3)N/APMK194S SRT-Tag Mouse Monoclonal Antibody(4H9)N/APMK194M SRT-Tag Mouse Monoclonal Antibody(4H9)N/APMK195S StrepTag II Mouse Monoclonal Antibody(Mix-mA™)N/APMK195M StrepTag II Mouse Monoclonal Antibody(Mix-mA™)N/APMK196S FH Fumarase Mouse Monoclonal Antibody(2B11)H,M,RPMK196M FH Fumarase Mouse Monoclonal Antibody(2B11)H,M,RPMK197S Flotillin-1 Mouse Monoclonal Antibody(6C10)M,R,(H)PMK197M Flotillin-1 Mouse Monoclonal Antibody(6C10)M,R,(H)PMK198S Progesterone Receptor Mouse Monoclonal Antibody(Z1H,M,RPMK198M Progesterone Receptor Mouse Monoclonal Antibody(Z1H,M,RPMK199S Vimentin Mouse Monoclonal Antibody(1A7)H,M,RPMK199M Vimentin Mouse Monoclonal Antibody(1A7)H,M,RPMK200S COX2 Cyclooxygenase 2 Mouse Monoclonal Antibody(3D H,M,RPMK200M COX2 Cyclooxygenase 2 Mouse Monoclonal Antibody(3D H,M,RPMK201S Lactoferrin Mouse Monoclonal Antibody(Q100)HPMK201M Lactoferrin Mouse Monoclonal Antibody(Q100)HPMK202S CD68 Mouse Monoclonal Antibody(12E2)H,M,RPMK202M CD68 Mouse Monoclonal Antibody(12E2)H,M,RPMK203S CREB Rabbit Polyclonal Antibody HPMK203M CREB Rabbit Polyclonal Antibody HPMK204S PRDX1 Rabbit Polyclonal Antibody H,M,RPMK204M PRDX1 Rabbit Polyclonal Antibody H,M,RPMK205S Lamin B1 Rabbit Polyclonal Antibody H,M,RPMK205M Lamin B1 Rabbit Polyclonal Antibody H,M,RPMK206S c-Fos Rabbit Polyclonal Antibody H,M,RPMK207M Vinculin Rabbit Polyclonal Antibody H,M,R PMK208S Histone H4 Rabbit Polyclonal Antibody H,M,R PMK208M Histone H4 Rabbit Polyclonal Antibody H,M,R PMK209S Histone H1 Rabbit Polyclonal Antibody H,M PMK209M Histone H1 Rabbit Polyclonal Antibody H,M PMK210Sα-Smooth Muscle Actin Rabbit Polyclonal Antibody H,M,R PMK210Mα-Smooth Muscle Actin Rabbit Polyclonal Antibody H,M,RH,M,R PMK211Sα-Skeletal Muscle Actin Rabbit Polyclonal AntibodyH,M,R PMK211Mα-Skeletal Muscle Actin Rabbit Polyclonal Antibody PMK212Sβ II Tubulin Rabbit Polyclonal Antibody H,M,R PMK212Mβ II Tubulin Rabbit Polyclonal Antibody H,M,R PMK213Sβ III Tubulin Rabbit Polyclonal Antibody H,M,R PMK213Mβ III Tubulin Rabbit Polyclonal Antibody H,M,R PMK214S Histone H2A.Z Rabbit Polyclonal Antibody M,R PMK214M Histone H2A.Z Rabbit Polyclonal Antibody M,R PMK215S GAP43 Rabbit Polyclonal Antibody H,M,R PMK215M GAP43 Rabbit Polyclonal Antibody H,M,R PMK216S Akt3 Rabbit Polyclonal Antibody H,M,R PMK216M Akt3 Rabbit Polyclonal Antibody H,M,R PMK217S c-Myc Rabbit Polyclonal Antibody H PMK217M c-Myc Rabbit Polyclonal Antibody H PMK218S Bovine Serum Albumin Rabbit Polyclonal Antibody Cow PMK218M Bovine Serum Albumin Rabbit Polyclonal Antibody Cow PMK219S Pan Methyl Lysine Rabbit Polyclonal Antibody N/A PMK219M Pan Methyl Lysine Rabbit Polyclonal Antibody N/A PMK220S Cytokeratin18 Rabbit Polyclonal Antibody H PMK220M Cytokeratin18 Rabbit Polyclonal Antibody H PMK221S Histone H2B Mouse Monoclonal Antibody(Mix-mA™)H,M,R PMK221M Histone H2B Mouse Monoclonal Antibody(Mix-mA™)H,M,R PMK222S mStrawberry Mouse Monoclonal Antibody(Mix-mA™)N/A PMK222M mStrawberry Mouse Monoclonal Antibody(Mix-mA™)N/A PMK223S eNOS Mouse Monoclonal Antibody(Mix-mA™)H,M,R PMK223M eNOS Mouse Monoclonal Antibody(Mix-mA™)H,M,R PMK224SPMK224MPMK225Sβ III tubulin Mouse Monoclonal Antibody(Mix-mA™)H,M,R PMK225Mβ III tubulin Mouse Monoclonal Antibody(Mix-mA™)H,M,R PMK226Sβ Tubulin Rabbit Polyclonal Antibody H,M,R PMK226Mβ Tubulin Rabbit Polyclonal Antibody H,M,R PMK227S Histone H3(Phospho Thr3 ) Rabbit Polyclonal Antibo H,M,R PMK227M Histone H3(Phospho Thr3 ) Rabbit Polyclonal Antibo H,M,R PMK228S Histone H3(Phospho Thr6 ) Rabbit Polyclonal Antibo H,M,R PMK228M Histone H3(Phospho Thr6 ) Rabbit Polyclonal Antibo H,M,R PMK229S Histone H3(Phospho Ser10 ) Rabbit Polyclonal Antib H,M,R PMK229M Histone H3(Phospho Ser10 ) Rabbit Polyclonal Antib H,M,R PMK230S Histone H3(Phospho Thr11 ) Rabbit Polyclonal Antib H,M,R PMK230M Histone H3(Phospho Thr11 ) Rabbit Polyclonal Antib H,M,R PMK231S Histone H3(Phospho Ser28 ) Rabbit Polyclonal Antib H,M,R PMK231M Histone H3(Phospho Ser28 ) Rabbit Polyclonal Antib H,M,R PMK232S Histone H3(Phospho Thr32 ) Rabbit Polyclonal Antib H,M,R PMK232M Histone H3(Phospho Thr32 ) Rabbit Polyclonal Antib H,M,R PMK233S Histone H3(Phospho Tyr41) Rabbit Polyclonal Antibo H,M,RPMK234M Histone H3(Phospho Thr45) Rabbit Polyclonal Antibo H,M,R PMK235S Histone H3(Phospho Thr118) Rabbit Polyclonal Antib H,M,R PMK235M Histone H3(Phospho Thr118) Rabbit Polyclonal Antib H,M,R PMK236S Histone H3 Rabbit Polyclonal Antibody H PMK236M Histone H3 Rabbit Polyclonal Antibody H PMK237S Histone H3(Acetyl Lys4 ) Rabbit Polyclonal Antibod H,M,R PMK237M Histone H3(Acetyl Lys4 ) Rabbit Polyclonal Antibod H,M,R PMK238S Histone H3(Acetyl Lys9 ) Rabbit Polyclonal Antibod H,M,R PMK238M Histone H3(Acetyl Lys9 ) Rabbit Polyclonal Antibod H,M,R PMK239S Histone H3(Acetyl Lys14 ) Rabbit Polyclonal Antibo H,M,R PMK239M Histone H3(Acetyl Lys14 ) Rabbit Polyclonal Antibo H,M,R PMK240S Histone H3(Acetyl Lys18 ) Rabbit Polyclonal Antibo H,M,R PMK240M Histone H3(Acetyl Lys18 ) Rabbit Polyclonal Antibo H,M,R PMK241S Histone H3(Acetyl Lys23 ) Rabbit Polyclonal Antibo H,M,R PMK241M Histone H3(Acetyl Lys23 ) Rabbit Polyclonal Antibo H,M,R PMK242S Histone H3(Acetyl Lys27 ) Rabbit Polyclonal Antibo H,M,R PMK242M Histone H3(Acetyl Lys27 ) Rabbit Polyclonal Antibo H,M,R PMK243S Histone H4(Phospho Ser1) Rabbit Polyclonal Antibod H,M,R PMK243M Histone H4(Phospho Ser1) Rabbit Polyclonal Antibod H,M,R PMK244S Histone H4(Phospho Ser47) Rabbit Polyclonal Antibo H,M,R PMK244M Histone H4(Phospho Ser47) Rabbit Polyclonal Antibo H,M,R PMK245S Histone H4(Phospho Thr80) Rabbit Polyclonal Antibo H,M,R PMK245M Histone H4(Phospho Thr80) Rabbit Polyclonal Antibo H,M,R PMK246S Histone H2B(Phospho Ser32) Rabbit Polyclonal Antib H,M,R PMK246M Histone H2B(Phospho Ser32) Rabbit Polyclonal Antib H,M,R PMK247S Histone H2A(Phospho Ser129) Rabbit Polyclonal Anti H,M,R PMK247M Histone H2A(Phospho Ser129) Rabbit Polyclonal Anti H,M,R PMK248S Histone H4(Di Methyl Lys20) Rabbit Polyclonal Anti H,M,R PMK248M Histone H4(Di Methyl Lys20) Rabbit Polyclonal Anti H,M,R PMK249S Histone H4(Tri Methyl Lys20) Rabbit Polyclonal Ant H,M,R PMK249M Histone H4(Tri Methyl Lys20) Rabbit Polyclonal Ant H,M,R PMK250S Histone H4(Di Methyl Lys59) Rabbit Polyclonal Anti H,M,R PMK250M Histone H4(Di Methyl Lys59) Rabbit Polyclonal Anti H,M,R PMK251S Histone H4(Tri Methyl Lys59) Rabbit Polyclonal Ant H,M,R PMK251M Histone H4(Tri Methyl Lys59) Rabbit Polyclonal Ant H,M,R PMK252S Histone H4(Mono Methyl Lys79) Rabbit Polyclonal An H,M,R PMK252M Histone H4(Mono Methyl Lys79) Rabbit Polyclonal An H,M,R PMK253S Histone H4(Di Methyl Lys79) Rabbit Polyclonal Anti H,M,R PMK253M Histone H4(Di Methyl Lys79) Rabbit Polyclonal Anti H,M,R PMK254S Histone H4(Tri Methyl Lys79) Rabbit Polyclonal Ant H,M,R PMK254M Histone H4(Tri Methyl Lys79) Rabbit Polyclonal Ant H,M,R PMK255S Histone H1(Mono Methyl Lys25) Rabbit Polyclonal An H,M,R PMK255M Histone H1(Mono Methyl Lys25) Rabbit Polyclonal An H,M,R PMK256S Histone H1(Di Methyl Lys25) Rabbit Polyclonal Anti H,M,R PMK256M Histone H1(Di Methyl Lys25) Rabbit Polyclonal Anti H,M,R PMK257S Histone H2B(Mono Methyl Lys5) Rabbit Polyclonal An H,M,R PMK257M Histone H2B(Mono Methyl Lys5) Rabbit Polyclonal An H,M,R PMK258S Histone H2B(Di Methyl Lys5) Rabbit Polyclonal Anti H,M,R PMK258M Histone H2B(Di Methyl Lys5) Rabbit Polyclonal Anti H,M,R PMK259S Histone H2B(Di Methyl Lys43) Rabbit Polyclonal Ant H,M,R PMK259M Histone H2B(Di Methyl Lys43) Rabbit Polyclonal Ant H,M,R PMK260S Histone H2B(Tri Methyl Lys43) Rabbit Polyclonal An H,M,R。