Modular-Batch-Type-Coater_Concept_Sunrain

SSPC-Paint 20 April 1, 2002
Editorial Revisions November 1, 2004
SSPC: The Society for Protective Coatings
PAINT SPECIFICATION NO. 20
Zinc-Rich Coating Type I – Inorganic and Type II – Organic
Paint 30
Weld-Through Inorganic Zinc
Primer
SP 1
Solvent Cleaning
SP 5/NACE No. 1 White Metal Blast Cleaning
PS Guide 12.00 Guide to Zinc-Rich Coating
Systems
* PS 12.01
One-Coat Zinc-Rich Painting
System
3.4 ASTM INTERNATIONAL STANDARDS:1
A 572 Standard Specification for High-Strength Low-Alloy Columbium-Vanadium Structural Steel
Level 1 — equal to or greater than 85% Level 2 — equal to or greater than 77% and less
than 85% Level 3 — equal to or greater than 65% and less
than 77%
1.5 This coating is intended for application by spray for use by itself or as a primer in a multi-coat system.

INSTRUCTIONSTMT Mass Tagging Kits and90060 TMTduplex Isotopic Label Reagent Set, sufficient reagents for 5 duplex isotopic experiments Contents:TMT0 Label Reagent, 5 × 0.8mgTMT6-127 Label Reagent, 5 × 0.8mg90061 TMTsixplex Isobaric Label Reagent Set, sufficient reagents for 1 sixplex isobaric experiment Contents:TMT6-126 Label Reagent, 1 × 0.8mgTMT6-127 Label Reagent, 1 × 0.8mgTMT6-128 Label Reagent, 1 × 0.8mgTMT6-129 Label Reagent, 1 × 0.8mgTMT6-130 Label Reagent, 1 × 0.8mgTMT6-131 Label Reagent, 1 × 0.8mg90062 TMTsixplex Isobaric Label Reagent Set, sufficient reagents for 2 sixplex isobaric experiments Contents:TMT6-126 Label Reagent, 2 × 0.8mgTMT6-127 Label Reagent, 2 × 0.8mgTMT6-128 Label Reagent, 2 × 0.8mgTMT6-129 Label Reagent, 2 × 0.8mgTMT6-130 Label Reagent, 2 × 0.8mgTMT6-131 Label Reagent, 2 × 0.8mg90063TMTduplex Isobaric Mass Tagging Kit, sufficient reagents for 5 duplex isobaric experiments Contents:TMT0 Label Reagent, 5 × 0.8mgTMT2-126 Label Reagent, 5 × 0.8mgTMT2-127 Label Reagent, 5 × 0.8mgDissolution Buffer (1 M triethyl ammonium bicarbonate), 5mLDenaturing Reagent (10% SDS), 1mLReducing Reagent (0.5M TCEP), 1mLIodoacetamide, 12 × 9mgQuenching Reagent (50% hydroxylamine), 1mLPierce™Trypsin Protease, MS Grade, 2 × 20µgTrypsin Storage Solution, 250µLAlbumin, Bovine, 2.5mg90064TMTsixplex Isobaric Mass Tagging Kit, sufficient reagents for 5 sixplex isobaric experiments Contents:TMT0 Label Reagent, 5 × 0.8mgTMT6-126 Label Reagent, 5 × 0.8mgTMT6-127 Label Reagent, 5 × 0.8mgTMT6-128 Label Reagent, 5 × 0.8mgTMT6-129 Label Reagent, 5 × 0.8mgTMT6-130 Label Reagent, 5 × 0.8mgTMT6-131 Label Reagent, 5 × 0.8mgDissolution Buffer (1M triethyl ammonium bicarbonate), 5mLDenaturing Reagent (10% SDS), 1mLReducing Reagent (0.5 M TCEP), 1mLIodoacetamide, 12 × 9mgQuenching Reagent (50% hydroxylamine), 1mLPierce Trypsin Protease, MS Grade, 5 × 20µgTrypsin Storage Solution, 250µLAlbumin, Bovine, 2.5mg90065TMTduplex Isobaric Label Reagent Set, sufficient reagents for 5 duplex isobaric experiments Contents:TMT2-126 Label Reagent, 5 × 0.8mgTMT2-127 Label Reagent, 5 × 0.8mg90066TMTsixplex Label Reagent Set, sufficient reagents for 5 sixplex isobaric experimentsContents:TMT6-126 Label Reagent, 5 × 0.8mgTMT6-127 Label Reagent, 5 × 0.8mgTMT6-128 Label Reagent, 5 × 0.8mgTMT6-129 Label Reagent, 5 × 0.8mgTMT6-130 Label Reagent, 5 × 0.8mgTMT6-131 Label Reagent, 5 × 0.8mg90067TMTzero Label Reagent, 5 × 0.8mg, sufficient reagents for 5 samples90068TMTsixplex Label Reagent Set, sufficient reagents for 12 sixplex isobaric experimentsContents:TMT6-126 Label Reagent, 2 × 5mgTMT6-127 Label Reagent, 2 × 5mgTMT6-128 Label Reagent, 2 × 5mgTMT6-129 Label Reagent, 2 × 5mgTMT6-130 Label Reagent, 2 × 5mgTMT6-131 Label Reagent, 2 × 5mgStorage: Upon receipt store at -20°C. Reagents are shipped with dry ice.Note: These products are for research use only − do not use for diagnostic procedures.ContentsIntroduction (3)Procedure Summary (4)Important Product Information (4)Additional Materials Required (4)Material Preparation (5)Preparing and Labeling Peptides with the TMT Isobaric Mass Tags (5)Troubleshooting (6)Additional Information (6)A.Data Acquisition Methods (6)B.Data Analysis and Quantitation (7)rmation Available from our Website (8)Related Thermo Scientific Products (8)General References (8)IntroductionThe Thermo Scientific™ TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex relative quantitation by mass spectrometry (MS). Each mass-tagging reagent within a set has the same nominal mass (i.e., isobaric) and chemical structure composed of an amine-reactive NHS-ester group, a spacer arm and an MS/MS reporter (Figure 1). The reagent sets can be used to label two or six peptide samples prepared from cells or tissues. For each sample, a unique reporter in the low mass region of the MS/MS spectrum (i.e., 126-127Da for TMT2 and 126-131Da for TMT6 Isobaric Label Reagents) is used to measure relative protein expression levels during peptide fragmentation.The TMTduplex™ Isotopic Label Reagent Set contains TMTzero™ and one of the TMTsixplex™ Reagents (TMT6-127) to be used as “light” and “heavy” tags for MS-level peptide quantitation similar to duplex isotopic metabolic labeling (e.g., SILAC) or isotopic dimethylation labeling. These isotopic pairs can also be used in targeted quantitation strategies, including selective reaction monitoring (SRM, see the Additional Information Section). Advantages of the TMTduplex and TMTsixplex Isobaric Label Reagents include increased sample multiplexing for relative quantitation, increased sample throughput and fewer missing quantitative channels among samples.Figure 1.Chemical structure of the TMTLabel Reagents. A. Functional regions of thereagent structure, including MS/MSfragmentation sites by higher energy collisiondissociation (HCD) and electron transferdissociation (ETD). B. TMTduplex Reagentstructures and isotope positions (*); only HCDdifferentiates between these two reporters.C. TMTsixplex Reagent structures and isotopepositions (*).Procedure SummaryProtein extracts isolated from cells or tissues are reduced, alkylated and digested overnight. Samples are labeled with the TMT Reagents and then mixed before sample fractionation and clean-up. Labeled samples are analyzed by high resolutionOrbitrap LC-MS/MS before data analysis to identify peptides and quantify reporter ion relative abundance (Figure 2).Figure 2. Schematic for using the Thermo Scientific TMTsixplex Isobaric Mass Tagging Reagents.Important Product Information• The TMT Reagents are moisture-sensitive. To avoid moisture condensation onto the product, vial must be equilibrated to room temperature before opening.•Anhydrous acetonitrile is the recommended solvent to dissolve reagents. Stock solutions are stable for one week when stored at -20°C. For long term storage of unused reagent, remove all solvent by drying and store with desiccant at -20°C. Anhydrous ethanol can be used as an alternative solvent to dissolve reagents but is not recommended for stock solution storage.• The TMT Reagents are amine-reactive and modify lysine residues and the peptide N-termini. All amine-containing buffers and additives must be removed before digestion and labeling.• All samples must be digested, labeled and then mixed equally before desalting, fractionation and LC-MS/MS. For optimal results, use 25-100µg of peptide for each labeling reaction.• To avoid contamination of MS samples, always wear gloves when handling samples and gels. Use ultrapure MS-grade reagents. Perform sample preparation in a cleaned work area.• The TMTzero Label Reagent can be used to optimize methods before multiplexed analysis of samples with the TMTduplex or TMTsixplex Reagent Set.Additional Materials Required• Microcentrifuge tubes• Anhydrous acetonitrile (Thermo Scientific™ Acetonitrile HPLC grade, Product No. 51101) • Water, LC-MS Grade (Product No. 51140) • Chilled (-20°C) acetone• Protein assay (e.g., Thermo Scientific™ BCA Protein Assay Kit, Product No. 22235) • 75-300µm capillary C 18 reversed-phase column• High-resolution Orbitrap Mass Spectrometer, ion trap or time-of-flight (TOF) mass spectrometer with online or offline liquid chromatography (LC) system• Data analysis software such as Thermo Scientific™ Proteome Discoverer™ or Mascot™ Software (Matrix Science, Ltd.)• Optional: C18 spin tips or columns (e.g., Thermo Scientific™ Pierce™ C18 Spin Columns, Product No. 89870 or Pierce™ C18 Tips, Product No. 87784)Material PreparationNote: The 50% hydroxylamine and 10% SDS stock solutions provided with the kit may precipitate during storage. Warm both solutions to room temperature and vortex before use. The amounts listed below are sufficient for preparing and labeling 6 samples.Add 500µL of the Dissolution Buffer (1M TEAB) to 4.5mL of ultrapure water.100mM TEAB (triethylammonium bicarbonate)Lysis Buffer Add 200µL of the Denaturing Reagent (10% SDS) to 1.8mL of 100mM TEAB.200mM TCEP Add 70µL of the Reducing Reagent (0.5M TCEP) to 70µL of ultrapure water. Then add 35µL of the Dissolution Buffer (1M TEAB).5% Hydroxylamine Add 50µL of the Quenching Reagent (50% hydroxylamine) to 450µL of 100mM TEAB. Preparing and Labeling Peptides with the TMT Isobaric Mass TagsNote: BSA can be used as a control sample for method optimization. Dissolve BSA to 1mg/mL using 100mM TEAB. Use 25-100µg of protein per labeling reaction. The Thermo Scientific™ Pierce™ Mass Spec Sample Prep Kit for Cultured Cells can also be used to prepare peptide digests for TMT reagent labeling.A.Preparing Whole Cell Protein Extracts1.Culture cells to harvest at least 100µg of protein per condition. For best results, culture a minimum of 2 × 106 cells.Note: Rinse cells 2-3 times with 1X PBS to remove cell culture media. Pellet cells using low-speed centrifugation(i.e., < 1000 × g) to prevent premature cell lysis.2.Lyse the cells by adding five cell-pellet volumes of Lysis Buffer (i.e., 100μL of Lysis Buffer for a 20μL cell pellet).Note: Lysis buffers such as 8M urea (Product No. 29700) in 50mM TEAB or HEPES buffer, pH 8 may be used as alternative denaturing cell lysis buffers. For urea-based lysis buffer, protein samples must be diluted to < 1M urea before digestion, and the final C18 desalting step (C.6) is not optional. Addition of protease and/or phosphatase inhibitors during lysis is optional and may interfere with MS analysis.Note: Depending on the Lysis Buffer used it may be necessary to reduce sample viscosity by shearing DNA using a microtip sonicator or addition of a nuclease (e.g., Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis, Product No. 88700)3.Centrifuge lysate at 16,000 × g for 10 minutes at 4°C.4.Carefully separate the supernatant and transfer into a new tube.5.Determine the protein concentration of the supernatant using established methods such as the BCA Protein Assay Kit(Product No. 23227).Note: Use samples at ≥ 2mg/mL. Less concentrated samples may be used; however, it might be necessary to use larger volumes of reducing/alkylating reagents.6.Transfer 100µg per condition (two for the TMTduplex or six for the TMTsixplex Label Reagents) into a newmicrocentrifuge tube and adjust to a final volume of 100µL with 100mM TEAB.7.Add 5µL of the 200mM TCEP and incubate sample at 55°C for 1 hour.8.Immediately before use, dissolve one tube of iodoacetamide (9mg) with 132µL of 100mM TEAB to make375mM iodoacetamide. Protect solution from light.9.Add 5µL of the 375mM iodoacetamide to the sample and incubate for 30 minutes protected from light at roomtemperature.10.Add six volumes (~600µL) of pre-chilled (-20°C) acetone and freeze at -20°C. Allow the precipitation to proceed for atleast 4 hours up to overnight.Note: Methanol/chloroform is the recommended solvent for precipitation of proteins derived from tissue extracts.11.Centrifuge the samples at 8000 ×g for 10 minutes at 4°C. Carefully invert the tubes to decant the acetone withoutdisturbing the white pellet. Allow the pellet to dry for 2-3 minutes.B.Protein Digestion1.Resuspend 100µg of acetone-precipitated (or lyophilized) protein pellets with 100µL of 50mM TEAB.Note: An acetone-precipitated pellet might not completely dissolve; however, after proteolysis at 37°C, all the protein (peptides) will be solubilized.2.Immediately before use, add 20µL of the Trypsin Storage Solution to the bottom of the trypsin glass vial and incubate for5 minutes. Store any remaining reagent in single-use volumes at -80°C (e.g., 2.5µg of trypsin per 100µg of protein).3.Add 2.5µL of trypsin (i.e., 2.5µg) per 100µg of protein. Digest the sample overnight at 37°C.C. Peptide Labeling1.Immediately before use, equilibrate the TMT Label Reagents to room temperature. For the 0.8mg vials, add 41µL ofanhydrous acetonitrile to each tube. For the 5mg vials, add 256µL of solvent to each tube. Allow the reagent to dissolve for 5 minutes with occasional vortexing. Briefly centrifuge the tube to gather the solution.Note: Reagents dissolved in anhydrous acetonitrile are stable for one week when stored at -20°C. Anhydrous ethanol can be used as an alternative solvent to dissolve reagents but is not recommended for stock solution storage.2.Optional: Measure protein digest concentration using Thermo Scientific™ Pierce™ Quantitative Fluorescent PeptideAssay (Product No. 23290) or Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay (Product No.23275).3.Carefully add 41µL of the TMT Label Reagent to each 100µL sample (25-100µg protein digest). Alternatively, transferthe reduced and alkylated protein digest to the TMT Reagent vial.4.Note: Labeling more than 100µg of protein digest per reaction requires additional TMT Label Reagent.5.Incubate the reaction for 1 hour at room temperature.6.Add 8µL of 5% hydroxylamine to the sample and incubate for 15 minutes to quench the reaction.bine samples at equal amounts in new microcentrifuge tube and store at -80°C.Note: TMT-labeled peptide concentration can be measured using Thermo Scientific™ Pierce™ QuantitativeColorimetric Peptide Assay. The Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay cannot be used to measure TMT-labeled peptide concentrations.8.Optional: Clean-up samples with C18 spin tips (Product No. 87784) or columns (Product No. 89870)before LC-MSanalysis. Peptide clean up is recommended before LC-MS analysis but is not required. Fractionation of labeled peptides using Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Product No. 84868) isrecommended before LC-MS analysis to increase the number of peptide identifications.TroubleshootingProblem Possible Cause SolutionPoor labeling An amine-based buffer was used Use a non-amine-based bufferIncorrect buffer pH Make sure the buffer pH is ~8.0Too much sample was used Label 25-100µg per sampleProtein precipitation Lack of detergent present Add detergent, such as 0.05% SDS to the preparationpH decreased Make sure the pH is > 7.5Additional InformationA.Data Acquisition MethodsQuantitation of peptides labeled with Thermo Scientific™ Tandem Mass Tag™ Reagents requires a mass spectrometer capable of MS/MS fragmentation, such as an ion trap, quadrupole time of flight, time of flight-time of flight (TOF-TOF) or triple quadrupole instrument. Higher energy collision dissociation (HCD) is recommended for TMT reporter ion fragmentation. Optimal HCD fragmentation energy is instrument-dependent and can be optimized using TMTzero Reagents.Electron transfer dissociation (ETD) may be used as an alternative fragmentation method for peptide identification and quantitation. The choice of MS/MS fragmentation method(s) depends on the instrument capabilities such as collisionally induced dissociation (CID), pulsed-Q dissociation (PQD), higher energy collisional dissociation (HCD), or electron transfer dissociation (ETD). TMT Reagent reporter ions are not visible in ion traps following traditional CID fragmentation.Table 1. Instruments and MS/MS fragmentation options for peptide identification and quantitation withThermo Scientific TMT Reagents.Instrument Fragmentation Method Reference(s)Thermo Scientific Orbitrap™ Fusion™ Tribrid™ Mass Spectrometer HCD/SPS-MS3 McAllister, G.C., et al. (2014), Viner,et al. (2013)Thermo Scientific Orbitrap Elite™ Mass Spectrometer HCD/MS3 McAllister, G.C., et al. (2012), Viner,et al. (2012)Thermo Scientific Q Exactive™ MassSpectrometerHCD/MS2 Wühr, et al. (2012)Thermo Scientific Orbitrap Velos Pro™, LTQ-Orbitrap™ XL, or MALDI-Orbitrap™ XL Mass Spectrometer HCD/MS2 Ting, et al. (2011), Wenger, et al(2011), Schirle, et al. (2012), Lee, etal (2011), Xiong, et al. (2011),Strupat, et al. (2008)Thermo Scientific™ Velos Pro™ ion trap Trap HCD/MS2 Biringer, et al. (2011)Thermo Scientific Orbitrap Elite ETD, Velos Pro ETD, LTQ-OrbitrapXL ETD HCD/MS2 orETD/MS2Viner, et al. (2009)Q-TOF CID Van Ulsen, et al. (2009)TOF-TOF CID Dayon, et al. (2008)Triple Quadrupole CID/SRM Stella, et al (2011), Byers, et al.(2009)B.Data Analysis and QuantitationThe masses for peptide modification by the TMT zero, duplex, and sixplex reagents are present in the UNIMOD database () and are listed below. Several software packages directly support the modifications by TMT Reagents and the relative quantitation of reporter ions released from labeled peptides, including Thermo Scientific™ Proteome Discoverer™ 1.1 and above, Matrix Science Mascot™ 2.1 and above, and Proteome Software Scaffold™ Q+. For data acquired using a combination of fragmentation methods (i.e., HCD/MS3 or HCD/ETD), Proteome Discoverer may be necessary to merge spectra for identification and quantitation.Table 2. Modification masses of the Thermo Scientific TMT Label Reagents.Label ReagentReagentReporter IonModificationMass(monoisotopic)ModificationMass(average)HCDMonoisotopicReporter Mass*ETDMonoisotopicReporter Mass**TMT0-126 126 224.152478 224.2994 126.127726 114.127725TMT2-126 126 225.155833 225.2921 126.127726 114.127725TMT2-127 127C 225.155833 225.2921 127.131081 114.127725TMT6-126 126 229.162932 229.2634 126.127726 114.127725TMT6-127 127N 229.162932 229.2634 127.124761 115.124760TMT6-128 128C 229.162932 229.2634 128.134436 116.134433TMT6-129 129N 229.162932 229.2634 129.131471 117.131468TMT6-130 130C 229.162932 229.2634 130.141145 118.141141TMT6-131 131 229.162932 229.2634 131.138180 119.138176 * HCD is a collisional fragmentation method that generates six unique reporter ions from 126 to 131Da.**ETD is a non-ergodic fragmentation method that generates six unique reporter ions from 114 to 119Da.rmation Available from our Website•Tech Tip Protocol #49: Acetone precipitation of proteins•Tech Tip Protocol #19: Remove detergent from protein samplesRelated Thermo Scientific Products90110 TMT10plex™ Isobaric Label Reagent Set, 10 × 0.8mg90113 TMT10plex Isobaric Mass Tag Labeling Kit90406 TMT10plex Isobaric Label Reagent Set, 10 × 5mg90114 1M Triethylammonium bicarbonate (TEAB), 50mL90115 50% Hydroxylamine, 5mL90100 iodoTMTzero™ Label Reagent, 5 × 0.2mg90101 iodoTMTsixplex™ Label Reagent Set, 1 × 0.2mg90103 iodoTMTsixplex Isobaric Mass Tag Labeling Kit90076 Immobilized Anti-TMT Antibody Resin90075 Anti-TMT Antibody, 0.1mL90104 TMT Elution Buffer, 20mL84840 Pierce™ Mass Spec Sample Prep Kit for Cultured Cells23227 BCA Protein Assay Kit23275 Pierce Quantitative Colorimetric Peptide Assay23290 Pierce Quantitative Fluorescent Peptide Assay90057 Pierce Trypsin Protease, MS Grade90051 Lys-C Protease, MS Grade88300 Fe-NTA Phosphopeptide Enrichment Kit88301 Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit84868 Pierce High pH Reversed-Phase Peptide Fractionation Kit88321 Pierce Peptide Retention Time Calibration Mixture, 200µL87784 Pierce C18 Tips, 100µL bed, 96 tips89870 Pierce C18 Spin Columns, 25 columns28904 Trifluoroacetic Acid, Sequanal GradeGeneral ReferencesAltelaar A.F., et al. (2012). Benchmarking stable isotope labeling based quantitative proteomics. J Proteomics Oct 22. pii: S1874-3919(12)00704-X.doi: 10.1016/j.jprot.2012.10.009.Bantscheff, M., et al. (2008). Robust and sensitive iTRAQ quantification on an LTQ Orbitrap Mass Spectrometer. Mol Cell Proteomics7:1702-13.Biringer, R.G., et al. (2011). Quantitation of TMT-Labeled Peptides Using Higher-Energy Collisional Dissociation on the Velos Pro Ion Trap Mass Spectrometer. Application note # 520. Byers, H.L. (2009). Candidate verification of iron-regulated Neisseria meningitidis proteins using isotopic versions of tandem mass tags (TMT) and single reaction monitoring, J Prot73(2):231-9.Dayon, L., et al. (2008). Relative quantification of proteins in human cerebrospinal fluids by MS/MS using 6-plex isobaric tags. Anal Chem80(8):2921-31. Dillon, R, et al. (2011). Discovery of a Novel B-Raf Fusion Protein Related to c-Met Drug Resistance. J Proteome Res10(11):5084-94.Erikson, B.K., et al. (2015). Evaluating multiplexed quantitative phosphopeptide analysis on a hybrid quadrupole mass filter/linear ion trap/orbitrap mass spectrometer. Anal Chem87(2):1241-9.Keshishian, H., et al. (2015). Multiplexed, quantitative workflow for sensitive biomarker discovery in plasma yields novel candidates for early myocardial injury. Mol Cell Proteomics. 2015 Feb 27. pii: mcp.M114.046813Lee, M.V., et al. (2011). A dynamic model of proteome changes reveals new roles for transcript alteration in yeast. Mol Syst Biol 7:514.McAllister, G.C., et al. (2014). MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across cancer cell line proteomes. Anal Chem86(14):7150-8.McAllister, G.C., et al. (2012). Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses. Anal Chem 84(17):7469-78.Murphy, J.P., et al. (2014). Combining amine metabolomics and quantitative proteomics of cancer cells using derivatization with isobaric tags. Proteomics 86(7):3585-93.Paulo, J.A., et al. (2014). A comprehensive proteomic and phosphoproteomic analysis of yeast deletion mutants of 14-3-3 orthologs and associated effects of rapamycin. Nature(2-3):474-86.Ross, P.L., et al. (2004). Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol Cell Proteomics 3(12):1154-69.Savitski, M.M., et al. (2014). Tracking cancer drugs in living cells by thermal profiling of the proteome. Science346(6205):1255784Schirle, M., et al. (2012). Kinase inhibitor profiling using chemoproteomics. Methods Mol Biol795:161-77.Schwartz, J. et al. (2008). Relative quantitation of protein digests using tandem mass tags and pulsed-Q dissociation (PQD). Application note # 452.Stella, R., et al. (2011). Relative Quantification of Membrane Proteins in Wild-type and PrP-knockout Cerebellar Granule Neurons. J Proteome Res doi: 10.1021/pr200759m. Strupat K., et al. (2008). Accurate MS and MSn Analysis with the Thermo Scientific MALDI LTQ Orbitrap. Application note # 30150.Ting, L., et al. (2011). MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics. Nature Methods8: 937–940.Van Ulsen, P., et al. (2009). Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach. Proteomics9(7):1771-81.Viner, R.I., et al. (2013). Increasing the multiplexing of protein quantitation from 6- to 10-Plex with reporter ion isotopologues.PN_ASMS_W617_RViner_R1.Viner, R.I., et al. (2012). Relative quantitation of TMT-labeled proteomes – Focus on sensitivity and precision. Application note #566.Viner, R.I., et al. (2009). Quantification of post-translationally modified peptides of bovine α-crystallin using tandem mass tags and electron transfer dissociation. J Proteomics72(5):874-85.Wenger, C.D., et al. (2011). Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging. Nat Methods 8(11):933-5. Xiong, L., et al. (2011). Mass spectrometric studies on epigenetic interaction networks in cell differentiation. J Biol Chem 286(15):13657-68.Zhang, T., et al. (2010). Improving quantitation of TMT-labeled peptides using stepped higher-energy collisional dissociation. Application note # 483 Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying package inserts (“Documentation”). No claim of suitability for use in applications regulated by FDA is made. The warranty provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than Buyer. Any model or sample furnished to Buyer is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.NO OTHER WARRANTIES, EXPRESS OR IMPLIED, ARE GRANTED, INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER’S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER’S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS.Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to humans or animals.Current product instructions are available at . For a faxed copy, call 800-874-3723 or contact your local distributor.© 2016 Thermo Fisher Scientific Inc. All rights reserved. Tandem Mass Tag and TMT are trademarks of Proteome Sciences plc. iTRAQ is a trademark of AB Sciex Pte. Ltd. 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ABSHUMID ACIDITY ANIL-API ANILCRV ANILPT ANIL-R API APICRV APICRVWT API-R APISTD APPMCON APPWCON AROMATIC AROMCRV APOM-V APOM-W ASPHACRV ASPHAL-R ASPHALTE AVAIL AVAILMX BASIC-N2 BAS-N2-R BAS-NCRV BETA BOD BODMX BROMICRV BROMINE C5-VC5-W CARBCRV CARBON CETANCRV CETANE CETANENO CETANE-R CETN4737 CHRATIO CHRATI-R CIDX4737 CIX976-1 CIX976-2 CLOUDCRV CLOUDPT CLOUD-R1 CLOUD-R2 CO2E-AR4 CO2E-SAR CO2E-US COCARBON COCARB-RCODMX COMB-O2 COPPER CP CPCV CPCVMX CPIG CPIGMX CPMX CPMX-M CSATMX CUTS-E CUTS-M CV CVMXD1160CRV D1160CVW D1160LV D1160T D1160TWT D1160WT D2887-10 D2887-30 D2887-5 D2887-50 D2887-70 D2887-90 D2887-95 D2887-C D2887CRV D2887T D2887WT D86-10 D86-30 D86-5D86-50 D86-70 D86-90 D86-95 D86CRK D86CRV D86CRVWT D86-FBP D86-IBP D86LVD86LVCK D86TD86TCKD86TWTD86TWTCK D86WTD86WTCKDG DGMIX DGMX DGPCDH DHMX DHPC DHVL DMX DON-C DRIVEIDX DRYCONC DS DSMX ELECCOND FAPP FAPP2 FLASHCRV FLASHPT FLPT-API FLPT-PM FLPT-R1 FLPT-R2 FLPT-R3 FLPT-R4 FLPT-TAG FREEZCRV FREEZEPT FREEZE-R FTRUEG GAMMA GAMPC GAMUS GAMUSAQ GIG GIGMX GMTRUE GMX GRVCRV GRVCRVWT GXS GXTRUE HH2ODEW-R HCDEWPT HFLMX HHV-0 HHV-15 HHVML-0 HHVML-15 HHVMS-0HIGMX HMX HNRYMX HXS HYDROCRV HYDROGEN IONSM IONSX IRON IRONCRV IRON-R K KINVISC KLL2 KMX KNOCKCRV KNOCKIDX KVISC KVISCCRV KVLKVL2 LFRAC LHV-0 LHV-15 LHVML-0 LHVML-15 LHVMS-0 LHVMS-15 LUMI-NO LUMINO-R LUM-NCRV MABP MABP-API MABP-R MAPP MAPP2 MASSCONC MASSFLAR MASSFLBR MASSFLC MASSFLCL MASSFLF MASSFLH MASSFLHE MASSFLI MASSFLMX MASSFLMX MASSFLO MASSFLOW MASSFLS MASSFRAC MASSFRARMASSFRC MASSFRCL MASSFRF MASSFRH MASSFRHE MASSFRI MASSFRN MASSFRO MASSFRS MASSHMX MASSRHO MASSRHOM MASSSFRA MASSSMX MASSVFRA MERCAPTA MERCCRV METAL METALCRV ML-LOAD MOCNCRV MOC-NO MOLECONC MOLEFLAR MOLEFLBR MOLEFLC MOLEFLCL MOLEFLF MOLEFLH MOLEFLHE MOLEFLI MOLEFLMX MOLEFLN MOLEFLO MOLEFLOW MOLEFLS MOLEFRAC MOLEFRAR MOLEFRBR MOLEFRC MOLEFRCL MOLEFRF MOLEFRH MOLEFRHE MOLEFRI MOLEFRN MOLEFRO MOLEFRS MON-C-R1 MON-C-R2 MON-C-R3 MON-LMU MUMX MVLSTD MVLSTDMX MVVSTD MVVSTDMX MW MWCRV MWCRVWT MWMXN+2A-V N+A-VN2-R NAPHCRV NAPHTHEN NAPHTH-V NAPHTH-W NICKCRV NICKEL NICKEL-R OLEFCRV OLEFIN OLEFIN-V OLEFIN-W OSMOT OXYGCRV OXYGEN PARACRV PARAFFIN PARAFF-V PARAFF-W PBUB PCMX PDEWPHPH25PH20TDEW PHI PHIMX PHIPC PHYDRATE PLPOH POH25 POURCRV POURP-R1 POURP-R2 POURPT PPMXPR PRES PRMX PRPT-APIRAT-VLFR RE REFICRV REFIDX-R REFINDEX REIDVP RELHUMID RHO RHOLSTD RHOMX RHOST-O RHOST-15 RHOSTD-R RIDX-API ROCNCRV ROC-NO RON-C-R1 RON-C-R2 RON-C-R3 RON-L RVPRVP-API RVP-ASTM RVPCRV RVP-P2 RVP-RS SFRAC SG SGAIR SG-R SGSTD SIG SIGMA SIGMAMX SIGMX SMOKCRV SMOKEPT SMOKPT-R SMX SODIUM SOLINDEX SONVEL SONVELMX SSOLFACT SSOLUB STRMCOST SULFCRV SULFUR SULFUR-WSUM-MSFR SUM-VLFR TBTBP-0 TBP-10 TBP-100 TBP-30 TBP-5 TBP-50 TBP-70 TBP-90 TBP-95 TBPCRV TBPCRVWT TBPLV TBPT TBPTWT TBPWT TBUB TCMX TDEW TEMP TFAPP TFAPP2 TFREEZ TFRZMARG TFTRUE TG THRMDIFF THYDRATE TLVCONC TM TOTAL-N2 TOT-NCRV TRMX TRUEFLOW TRUEFRAC TRUEMW TWAPP TWAPP2 TWTRUE UUMX UOPKCRV VVABP VACCRV VACCRVWT VACLV VACTVACWT VANACRV VANADIUM VANA-R VCMX VFRAC VISC VISCCRV VISINDEX VLOCKCRV VLOCKIDX VLSTD VLSTDFR VLSTDMX VMX VOLFLMX VOLFRAC VOLHMX VVSTD VVSTDFR VVSTDMX WAPP WAPP2 WARMICRV WARMIDX WAT WATER WAT-R WAX WOBBE-0 WOBBE-15 WT-LOAD WTRUE WXAPP WXAPP2 WXTRUE XAPP XAPP2 XEVAP XTRUE ZCMX ZMX绝对湿度在给定的液相体积百分比下，石油混合物的酸度，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可用）基于API方法的石油混合物的苯胺点石油混合物的苯胺点曲线，仅在Assay Data Analysis 中指定数据时，才可请求物性组石油混合物的苯胺点，仅在Assay Data Analysis 中指定数据时，才可请求物性值石油混合物的苯胺点，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可用）石油混合物的API比重指数基于液相体积的石油混合物的API比重指数曲线基于重量的石油混合物的API比重指数曲线石油混合物的API比重指数,RefSYS 关联式物性石油混合物的API比重指数(同API)液相混合物中指定组分的表观摩尔浓度，针对指定组分的表观摩尔流率的总和对基于表观摩尔分率的指定液相混合物中指定组分的表观质量浓度，针对指定组分的表观质量流率的总和对基于表观摩尔分率的指定基于重量的石油混合物的芳烃含量，仅在Assay Data Analysis 中指定数据时，才可请求物性组基于重量的石油混合物的芳烃含量曲线，仅在Assay Data Analysis 中指定数据时，才可请求物性组在给定的液相体积百分比下，石油混合物的芳烃含量，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可在给定的重量百分比下，石油混合物的芳烃含量，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可用）基于重量的石油混合物的沥青质含量，仅在Assay Data Analysis 中指定数据时，才可请求物性组在给定的重量百分比下，石油混合物的沥青含量，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可用）基于重量的石油混合物的沥青质含量，仅在Assay Data Analysis 中指定数据时，才可请求物性组纯组分的有效能：H-ToS 其中To=298.15K混合物的有效能：H-ToS 其中To=298.15K基于重量的石油混合物的基本氮含量，仅在Assay Data Analysis 中指定数据时，才可请求物性组在给定的重量百分比下，石油混合物的基本氮含量，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可用基于重量的石油混合物的基本氮含量曲线，仅在Assay Data Analysis 中指定数据时，才可请求物性组混合物中第一个液相占全液相的分数纯组分的生物化学氧需求混合物的生物化学氧需求石油混合物的溴值曲线，仅在Assay Data Analysis 中指定数据时，才可请求物性组石油混合物的溴值，仅在Assay Data Analysis 中指定数据时，才可请求物性组在给定的液相体积百分比下，石油混合物的C5含量，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可用在给定的重量百分比下，石油混合物的C5含量，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时石油混合物的残炭曲线，仅在Assay Data Analysis 中指定数据时，才可请求物性组石油混合物的残炭，仅在Assay Data Analysis 中指定数据时，才可请求物性组石油混合物的十六烷值曲线，仅在Assay Data Analysis 中指定数据时，才可请求物性组石油混合物的十六烷值，仅在Assay Data Analysis 中指定数据时，才可请求物性组石油混合物的十六烷值在给定的重量百分比下，石油混合物的十六烷值，RefSYS 关联式物性式石油混合物的十六烷值（D4737）,RefSYS 关联式物性石油混合物的碳氢比在给定的重量百分比下，石油混合物的碳氢比，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时石油混合物的十六烷指数（D4737）,RefSYS 关联式物性石油混合物的十六烷指数（D976，方法1）,RefSYS 关联式物性石油混合物的十六烷指数（D976，方法2）,RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可用）石油混合物的蚀点曲线，仅在Assay Data Analysis 中指定数据时，才可请求物性值石油混合物的蚀点，仅在Assay Data Analysis 中指定数据时，才可请求物性值石油混合物的蚀点（方法1），RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可用）石油混合物的蚀点（方法2），RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时可用）碳当量-IFPP第四评估报告（2007）碳当量-IFPP第二评估报告（1995）碳当量-US EPA（2009）基于重量的石油混合物的康拉特逊碳含量，仅在Assay Data Analysis 中指定数据时，才可请求物性值在给定的重量百分比下，石油混合物的康拉特逊碳含量，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时纯组分的化学氧需求化合物的化学氧需求完全燃烧给定材料的需氧量在给定的重量百分比下，石油混合物的铜含量，RefSYS 关联式物性（仅当运行Aspen HYSYS Refining 时纯组分的恒压热容纯组分的热容比率（CP/CV）混合物的热容比率（CPMX/CVMX）纯组分的理想气体热容混合物的理想气体热容混合物的恒压热容混合物的恒压质量热容饱和混合物的比热100华氏温度区间内石油馏分及轻端馏分的流率50华氏温度区间内石油馏分及轻端馏分的流率纯组分的恒容热容混合物的恒容体积热容多个压力下，基于液相体积的使用混合物的ASTM D1160蒸馏曲线，当在Qualifer 页上选择 Sysytem pressure 框多个压力下，基于重量的石油混合物的ASTM D1160蒸馏曲线，当在Qualifer 页上选择 Sysytem pressure 框时，多个压力下，石油混合物的ASTM D1160液相体积百分比，当在Qualifer 页上选择 Sysytem pressure 框时多个压力下，石油混合物的ASTM D1160的温度，当在Qualifer 页上选择 Sysytem pressure 框时，Defau多个压力下，石油混合物的ASTM D1160的温度，当在Qualifer 页上选择 Sysytem pressure 框时，Defau多个压力下，石油混合物的ASTM D1160重量百分比，当在Qualifer 页上选择 Sysytem pressure 框时，D在10%的重量百分比下，石油混合物的 ASTM D2887 温度，RefSYS 物性关联式。

通过CFD模拟仿真Compax TM 混合器 中湍流混合漩涡
0605 2701
引导增强工艺技术的先锋
混合 不同流量，密度，粘度及其它特性的易溶体的混合。
006691 2726--0044
分散与接触 分散两个或多个产品用于工艺洗涤、反应、传质。 气液接触增加传质表面积并提高吸收率、反应率、 气化和浓缩率。
泵
0691 2724-03
静态混合器
0606 2722
通过激光诱导荧光测量的苏尔寿 SMX TM中的 层流混合
湍流混合 即使在空管中也存在一些湍流混合。 通过安装静态混合器，实现出色的均 一化效果所要求的距离缩小10倍以上。 每个静态混合器均具有一个或几个混 合单元在流动中产生旋转的涡流。任 何形式的非均相通过混合单元的分割 以及下游涡流都可以实现充分混合。
．．．化工工艺设备
4588 4059
客户的受益 均衡一致的工艺条件提供了均匀的： －浓度 －温度 －停留时间分布
－均一化产品质量 －高收益 －对连续性反应的高选择性 －低能耗 －缩小混合／ 反应器的体积 －减小工艺流程空间 －低维护成本 －开车、操作简单
主要应用 液体稀释 添加剂添加 气液流调控 非互溶液体分散 液体气化 气液接触 气体在液体中充分溶解
0685 2019-01
0606 2702
调控 调控反应器和换热器的逆流工艺旨在 增效。
热交换与反应
可以控制反应的热交换，提高高粘度 物料的热量转化或温和的温度变化。 反应在同一停留时间分布和高驱动力 下的平推流中进行。
0606 2723-1
苏尔寿 SMVTM 和湍流状态用的混合器
传质与反应过程中湍流状态的混合，分散及接触 低粘度的气液混合和均匀化 混合低粘度的添加剂