一株茄病镰刀菌的代谢产物研究_张梅
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茄科蔬菜苯丙烷类代谢及相关酶基因研究进展发表时间:2020-07-02T06:06:53.381Z 来源:《防护工程》2020年9期作者:田瑞新李骥鸿胡延贝张伶张娟苏海峰[导读] 但一些作物的转基因体系尚未成熟,多数基因功能与遗传机制尚未明确。
山东旺天下农业旅游发展有限公司山东菏泽 274100摘要:茄科蔬菜遗传背景狭窄,通过杂交选育获得高含量有益代谢物和高抗等育种材料,利用分离遗传群体作图挖掘目的基因或QTL 存在一定局限性,诱变育种是创新种质的重要途径。
在茄科蔬菜中已筛选出花青素和绿原酸等苯丙烷类代谢产物的EMS突变体和候选基因,但一些作物的转基因体系尚未成熟,多数基因功能与遗传机制尚未明确。
关键词:茄科蔬菜苯丙烷类代谢;酶基因随着次生代谢产物在医药、食品、轻化工等领域的广泛应用,其物质的种类、代谢途径,以及代谢机理等相关问题亦倍受研究者关注,是植物生理学、植物化学等众多学科的主要研究内容之一。
植物次生代谢物的产生和分布通常有种属、器官组织和生长发育期的特异性。
一、苯丙烷类苯丙烷类或其衍生物广泛分布于约250 000种维管植物中,结构迥异,种类繁多,广泛参与调节生长发育、繁殖和防御等各种植物生理活动。
黄酮类化合物泛指由2个芳香环(A和B)通过中央三碳链相互连接而成的以苯色酮环为基础结构的一系列化合物,目前已发现4500多种异型分子,苯丙烷类化合物生物合成的起始分子为芳香族氨基酸,即苯丙氨酸和酪氨酸。
研究表明,在大多数植物苯丙烷类化合物代谢途径中包含两个基本途径,即莽草酸途径和丙二酸途径。
莽草酸途径主要参与高等植物的苯丙烷类代谢,丙二酸途径则为真菌或细菌的合成途径。
在高等植物体中,通过莽草酸途径可将赤藓糖-4-磷酸(磷酸戊糖途径)与磷酸烯醇式丙酮酸(糖酵解途径)结合经中产物莽草酸(故名为“莽草酸途径”)转化为芳香族氨基酸———苯丙氨酸和酪氨酸。
这两种芳香族氨基酸为苯丙烷类化合物生物合成的起始分子。
J 1SHANXI AGRIC 1UNIV 1(N at ural S cience Edition )学报(自然科学版)2009,29(1)002473收稿日期:2008203205 修回日期:2008206224作者简介:李蕊倩(19672),女(汉),山西万荣人,讲师,硕士,主要从事成人教育管理和植物病理方面的研究。
基金项目:山西省留学基金(2004048)细辛提取物对镰刀菌抑菌作用的初步分析李蕊倩(山西农业大学成人教育学院,山西太谷030801)摘 要:为了研究细辛对镰刀菌的抑制作用及机理,采用生长速率法和孢子萌发法测定了细辛对镰刀菌的抑菌作用,采用电导法研究了细辛对镰刀菌细胞膜的通透性影响。
结果表明:细辛对镰刀菌菌丝生长和孢子萌发有很好的抑制作用,且菌丝比孢子对细辛提取物的敏感性更强。
电导率测定结果表明,细辛提取物处理的镰刀菌的电导率值显著高于对照组,说明了细辛提取物可以破坏镰刀菌的细胞膜选择通透性,导致内容物外渗,细胞膜是细辛提取物的作用靶点之一。
关键词:细辛;镰刀菌;抑菌作用中图分类号:S476 文献标识码:A 文章编号:167128151(2009)0120059203Inhibitory E ffect of As a rum heterot ropoi des Against Fusa rium spp.LI Rui 2qian(College of A dult Education ,S hanx i A g ricultural Universit y ,Tai gu S hanx i 030801,China )Abstract :The inhibitory effect of A sarum heterot ropoi des against Fusari um ,the toxicity on the mycelium and spores and cell membrane permeability of Fusari um hypha were studied in the paper.The results showed that the inhibitory effect of A sarum heterot ropoi des on the Fusari um spp 1is good.And the inhibitory effect of A sarum heterot ropoi des a 2gainst the growth of mycelium is more obvious to the growth of the spore.At the same time ,the conductivity of cell membrane is obviously higher than control in the different time ;the electrical conductivity rates is increased obviously with the medicine concentration enhancement and treat time lengthening.That sugqested that the cell membrane per 2meability were destroyed after treated with A sarum heterot ropoi des and substances in the cell were leaked outside.All these results showed that cell membrane was the one of targets attacked by A sarum heterot ropoi des .K ey w ords :A sarum heterot ropoi des ;Fusari um ;Inhibitory effect 细辛(A sarum heterot ropoi des var m an ds hu 2ricum )为马兜铃科(Aristolochiaceae )草本植物,是一味常用中药,其始载本草为《神农本草经》,具有祛风解表、散寒止痛、温肺化饮、宣通鼻窍的功效[1]。
马特组镰刀菌21株马特组镰刀菌遗传多样性的ISSR分析*李新凤1张光明1畅引东1王建明1**李蕊倩2李亚立1(1山西农业大学农学院,山西太谷030801;2山西农业大学成人教育学院,山西太谷030801)摘要为明确马特组镰刀菌种间和种内的遗传差异与亲缘关系,本文利用ISSR分子标记技术对21个马特组菌株进行了遗传多样性分析。
利用筛选出的15条引物对3种供试菌株进行扩增,共扩增出239条条带,其中多态性条带230条,多态性位点比例为96.2%,平均每条引物产生条带数为15.3条。
聚类分析和遗传相似系数分析结果表明,21个菌株间的遗传相似系数范围为0.494~0.933,平均为0.640。
在遗传相似系数为0.593时,供试的21株马特组镰刀菌可明显分成2个ISSR类群(IG),IG-Ⅰ包括1~17号菌株,为Fusarium solani和F. solani var. coeruleum;IG-Ⅱ包括18~21号菌株,全部为F.ventricosum;在遗传相似系数为0.933时,供试的21个菌株可被全部区分开。
ISSR分析结果表明,供试的镰刀菌基因组在SSR区域具有丰富的多态性;ISSR类群划分与菌种分类之间存在一定相关性,但与菌株的地理来源没有相关性;而同一类群中,不同菌株之间的遗传相似性与菌株的地理来源存在一定的相关性。
同一地区同种寄主的相同菌种,其菌株间也存在一定的遗传差异。
关键词马特组镰刀菌分子标记ISSR-PCR遗传多样性中图分类号S43ISSR analysis of genetic diversity of 21 Fusarium strains in Section Martiella. LI Xin-feng1, ZHANG Guang-ming1, CHANG Yin-dong1, WANG Jian-ming1, LI Rui-qian2, LI Ya-li1 (1College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China;2College of Adult Education, Shanxi Agricultural University, Taigu 030801, Shanxi, China)Abstract: The genetic diversity of 21 Fusarium strains inSection Martiella were examined by inter-simple sequence repeats (ISSR) to investigate genetic difference and phylogeny relationship within and among species. A total of 239 bands were amplified with 15 selected ISSR primers, 230 (96.2%) of which were polymorphic, with average of 15.3 polymorphic bands per primer. The cluster analysis based on the ISSR data showed that the genetic similarity ranged from 0.494 to 0.933, with the average of 0.640. All the strains tested were clustered into two groups at 0.593 genetic similarity. Strains 1 to 17 were grouped into IG-I, which were Fusarium solani and F. solani var. coeruleum, while the other 4 strains were grouped into IG-II and all of them were F. ventricosum. All the strains could be distinguished at 0.933. The results indicated that the SSR loci in Fusarium genomes were rich in polymorphism. ISSR groups showed a partial correlation with morphological species, and no significant correlation with geographic origin of the strains, while there was some correlation between genetic similarity and geographic origin of strains in the same ISSR groups. Some genetic diversity was observed among the strains of the same species, even they were collected from the same region and their host plants were of the same species.Keywords: Fusarium strains; Section Martiella; molecular marker; ISSR-PCR; genetic diversity.镰刀菌属(Fusarium Link)是一类具有重要经济意义的真菌,该属在自然界中分布广、数量多、变异快,因而其分类较复杂[1]。
植物根际促生菌的促生机制与应用摘要:植物根际促生菌(Plant Growth Promoting Rhizobacteria,PGPR),因其能直接改变土壤元素形态、分泌生长素及抑制植物病原菌,从而促进植物生长发育,提高作物产量。
本文重点梳理了PGPR在促进植物生长、增强植物抗逆性、抑制植物病原体及实际应用的功能方面进行了介绍,旨在为今后PGPR的研究和应用提供理论参考。
关键词:PGPR;促生机制;应用1 前言在植物根系代谢过程中,周围被土壤覆盖的区域称为根际,关系到植物根系生长和活动的非常重要的区域。
生长或附着在植物根部的菌株可以促进植物的生长发育,抑制有害病原体的生长,这类微生物被称为植物根际促生菌(Plant Growth Promoting Rhizobacteria,PGPR)[1]。
PGPR通过直接或间接的方式对植物生长发育起到促进作用。
通过其分泌生长激素或改变土壤元素形态对植物生长起到直接促生作用,或者通过抑制植物病原体或降解有害物质,间接促进植物生长。
2 PGPR的促生机制2.1直接机制PGPR通过固氮、矿质养分的溶解和植物激素的产生来提高植物对土壤养分的吸收,直接影响植物生长活性,并根据PGPR的种类、性能强度和植物的种类变化而变化。
2.1.1 固氮生物固氮是一个惊人的过程,约占全球固氮量的三分之二,通过微生物与植物的共生或非共生作用发生的。
氮是植物生长发育最重要的元素之一,在生长和各种代谢活动中起着重要作用。
PGPR将空气中的游离氮转化为氨,然后被植物吸收利用。
PGPR未来将成为植物氮元素管理越来越重要的组成部分。
化学氮肥只有约30-50%被植物吸收,剩余吸附在土壤的有机氮变得不可吸收或浸入自然环境中流失,造成土壤污染。
而PGPR的菌肥菌剂具有环境友好型特点,更有利于环境发展和农业可持续发展.2.1.2 磷酸盐增溶磷是仅次于氮的植物生长发育第二重要元素。
土壤中磷常以不溶或难溶性的形式存在(例如磷酸钙和磷酸锌等),导致植物根系很难吸收。
中国农学通报2016,32(6):198-204Chinese Agricultural Science Bulletin一株生防细菌GB58的鉴定与抑菌能力测定卢钰升1,顾文杰1,蒋瑞萍1,孙丽丽1,徐培智1,谭志远2,解开治1,李文英1,李夏1(1广东省农业科学院农业资源与环境研究所,农业部南方植物营养与肥料重点实验室,广东省养分资源循环利用与耕地保育重点实验室,广州510642;2华南农业大学农学院,广州510642)摘要:对从广东水稻土中获得能高效抑制植物病原菌的细菌GB58进行鉴定、抑菌广谱性测定,明确其潜在应用价值。
采用平板对峙法测定GB58抑菌广谱性,并参照细菌系统鉴定方法,通过形态、生理生化、分子生物学手段鉴定菌株。
抗菌广谱性试验结果表明:GB58对多达12种的植物真菌性病害和1种细菌性病害具有较好的拮抗作用。
细菌常规鉴定试验结果显示:GB58为芽孢杆菌属,碳源利用分析表明GB58能够利用46种碳源物质以及耐酸耐盐,通过16S rRNA、gyrA序列进行系统发育分析,确定GB58为解淀粉芽孢杆菌。
GB58是一株抗菌范围广,营养竞争能力强的生防细菌,具备适应华南酸性土壤复杂环境的繁殖和代谢能力。
关键词:生防细菌;鉴定;系统发育;抗菌广谱性中图分类号:S182,S432.4文献标志码:A论文编号:casb15100004Identification and Antimicrobial Broad-spectrum Analysis of a Biocontrol Bacteria Strain GB58Lu Yusheng1,Gu Wenjie1,Jiang Ruiping1,Sun Lili1,Xu Peizhi1,Tan Zhiyuan2,Xie Kaizhi1,Li Wenying1,Li Xia1 (1Institute of Agricultural Resources and Environment,Guangdong Academy of Agricultural Sciences,Key Laboratory of Plant Nutrition and Fertilizer in South Region,Ministry of Agriculture,Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation,Guangzhou510642;2College of Agriculture,South China Agricultural University,Guangzhou510642)Abstract:This article presented the identification and antimicrobial broad-spectrum analysis of the biocontrol bacteria strain GB58obtained in rice soil of Guangdong Province,which could inhibit plant pathogens,and aimed to clarify its potential application value.The antibacterial broad-spectrum of GB58was determined by the plate confrontation method,and the bacterial strain was identified by morphological,physiological, biochemical and molecular biology methods which were the reference for bacteria system identification.The antimicrobial broad-spectrum result showed that GB58had better antagonism to twelve plant fungal diseases and one plant bacterial disease.In the meantime,common bacterial identification test showed that GB58was bacillus and the carbon utilization analysis showed that GB58could utilize46kinds of carbon sources and was able to tolerate acid and salt.Moreover,the phylogenetic analysis through16S rRNA and gyrA sequence concluded that GB58was bacillus amyloliquefaciens.All the results suggested that GB58was a biocontrol bacteria strain with wide antibacterial range and high nutrition competitive ability,which had the ability of基金项目:广东省科技计划项目“基于菜园退化土壤的功能性微生物接种剂研发与应用”(2013B020310001);广东省科技计划项目“橄榄有机无机专用肥的研制与应用”(2013B020419009);广东省科技计划项目“广东省新型肥料与高效施肥工程技术研究中心建设”(2014B090904068);广东省科技计划项目“广东省新型肥料科技创新与服务平台建设”;广东省财政厅项目“多功能生物复合型有机营养液肥的研发与应用”;广州市农业局农业科技攻关招标项目“城郊结合处集约化畜禽养殖场固体废弃物无害化与资源化综合利用研究”(GZCQC0902FG06017)。
河南烟草镰刀菌的分子鉴定及致病性分析邱睿;白静科;李成军;李淑君;李小杰;陈玉国;胡亚静;刘东升【摘要】为明确河南省不同烟叶产区危害烟草的镰刀菌种类,以河南省烟叶产区典型镰刀菌根腐病烟株及烟田土壤为供试样品,采用组织分离法和土壤分离法获得纯化菌株 ,根据菌株形态和rDNA-ITS序列分析对病原菌进行鉴定 ,并采用无创接种法测定病原菌对烟草的致病性.结果表明 ,获得的98个菌株在PDA培养基上均产生白色菌丝,大型分生孢子呈镰刀形或马特形,无色、多孢;小型分生孢子呈肾形、椭圆形或卵形,无色、多无分隔.序列比对结果显示,所分离的98株菌分属尖孢镰刀菌(Fusarium oxysporum)、茄病镰刀菌(Fusarium solani)、层出镰刀菌(Fusarium proliferatum)、木贼镰刀菌(Fusarium equiseti)和Fusarium nematophilum.致病性分析结果表明,尖孢镰刀菌、茄病镰刀菌、层出镰刀菌和木贼镰刀菌对烟苗具有致病性;48株致病菌中豫南烟区烟草镰刀菌根腐病致病性病原菌为尖孢镰刀菌、层出镰刀菌和木贼镰刀菌,以尖孢镰刀菌为主;豫中为尖孢镰刀菌;豫东、豫西为茄病镰刀菌和尖孢镰刀菌.因此,尖孢镰刀菌为全省烟区重点防控对象,豫南烟区还需加强层出镰刀菌和木贼镰刀菌的防控,豫东、豫西需防范茄病镰刀菌的发生.%To identify Fusarium species in different tobacco producing areas of Henan province,tobacco plants with typical symptoms of fusarium root rot and fi eld soil were sampled to isolate pathogens by tissue separation method and soil separation method. Pure strains were identified by morphological characteristics and rDNA-ITS sequence analysis, and their pathogenicities were determined by non-invasive inoculation method. Morphological observation showed that on PDA medium, all of the 98 purifi ed stains produced white hyphae, macroconidium was sickle or matt in shape,colorless and multi-cell; microconidium was reniform, ovoid or oval in shape, colorless and most of them were single-cell. Sequence alignment indicated that the 98 isolated strains were Fusarium oxysporum, Fusarium solani, Fusarium proliferatum,Fusarium equiseti and Fusarium nematophilum.Pathogenicity analysis showed that 48 strains were pathogenic to tobacco seedlings,which wereF.oxysporum,F.solani,F.proliferatum and F.equiseti.In Southern Henan,the pathogen of fusarium root rot were F.oxysporum,F.proliferatum andF.equiseti,with F.oxysporum the predominant pathogen.F.oxysporum was the pathogenic fungi in Centrol Henan,and F.solani and F.oxysporum were the pathogenic stains in Eastern and WesternHenan.Therefore,F.oxysporum was the key prevention and control objects in all tobacco planting area of Hennan province,and control ofF.proliferatum and F.proliferatum need to be strengthened in Southern Henan,and strict precautions measure be taken against the occurrence of F.solani.in Eastern and Western Henan.【期刊名称】《中国烟草学报》【年(卷),期】2018(024)002【总页数】6页(P129-134)【关键词】河南省;烟草;镰刀菌;分子鉴定;致病性【作者】邱睿;白静科;李成军;李淑君;李小杰;陈玉国;胡亚静;刘东升【作者单位】河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南省许昌市永昌大道与青梅路交叉口 461000;河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南省许昌市永昌大道与青梅路交叉口 461000;河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南省许昌市永昌大道与青梅路交叉口 461000;河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南省许昌市永昌大道与青梅路交叉口 461000;河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南省许昌市永昌大道与青梅路交叉口 461000;河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南省许昌市永昌大道与青梅路交叉口 461000;河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南省许昌市永昌大道与青梅路交叉口 461000;河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南省许昌市永昌大道与青梅路交叉口 461000【正文语种】中文镰刀菌(Fusarium spp.)是一类世界性分布的真菌,可以侵染多种植物,引起植物的根腐、茎腐、花腐和穗腐等多种病害。
一株烟草花叶病毒生防细菌的鉴定及代谢物活性研究申莉莉;王凤龙;钱玉梅;杨金广;陈德鑫;张爱东;张杨【期刊名称】《中国烟草科学》【年(卷),期】2009(30)6【摘要】从烟田土样中分离到1株对TMV有拮抗作用的细菌By33,通过形态观察、生理生化试验及16S rDNA同源性序列分析,鉴定该菌为解淀粉芽孢杆菌.通过对By33代谢产物对TMV抑制作用测定发现,其代谢物质对温度敏感,80℃处理30 min,丧失大部分活性,遇酸沉淀,碱性条件下稳定,对蛋白酶不敏感.【总页数】4页(P65-68)【作者】申莉莉;王凤龙;钱玉梅;杨金广;陈德鑫;张爱东;张杨【作者单位】农业部烟草类作物质量控制重点开放实验室,中国农业科学院烟草研究所,青岛,266101;农业部烟草类作物质量控制重点开放实验室,中国农业科学院烟草研究所,青岛,266101;农业部烟草类作物质量控制重点开放实验室,中国农业科学院烟草研究所,青岛,266101;农业部烟草类作物质量控制重点开放实验室,中国农业科学院烟草研究所,青岛,266101;农业部烟草类作物质量控制重点开放实验室,中国农业科学院烟草研究所,青岛,266101;山东潍坊烟草有限公司,山东,潍坊,261000;山东中烟工业公司,济南,250013【正文语种】中文【中图分类】S435.72【相关文献】1.一株辣椒枯萎病生防细菌L-1的分离与鉴定 [J], 巩文峰;邢瑜琪;张荣;旺姆;岳海梅;;;;;;2.一株生防细菌LHB02的筛选、鉴定及其发酵条件优化(英文) [J], 徐长安;唐旭;何建林;林凌;刘源森;方卫东;3.一株生防细菌LHB02的筛选、鉴定及其发酵条件优化 [J], 徐长安; 唐旭; 何建林; 林凌; 刘源森; 方卫东4.一株辣椒枯萎病生防细菌L-1的分离与鉴定 [J], 巩文峰[1];邢瑜琪[1];张荣[2];旺姆[1];岳海梅[1]5.一株生防细菌GB58的鉴定与抑菌能力测定 [J], 卢钰升;顾文杰;蒋瑞萍;孙丽丽;徐培智;谭志远;解开治;李文英;李夏因版权原因,仅展示原文概要,查看原文内容请购买。
三七根系分泌物中几种成分对根腐病原菌生长的影响作者:罗丽芬江冰冰邓琳梅梅馨月黄惠川刘屹湘杨敏朱书生来源:《南方农业学报》2020年第12期摘要:【目的】探究三七根系分泌物对根腐病原菌生长的影响,为解析三七根系分泌物介导的植物与土传病原菌互作机制提供理论依据。
【方法】通过根系与病原菌孢子互作的方式研究三七根系对根腐病原菌茄腐镰刀菌F3(Fusarium solani)和恶疫霉菌D-1(Phytophthora cactorum)孢子萌发及芽管生长方向的影响;收集三七根系分泌物并利用气相色谱—飞行时间质谱联用仪(GC-TOF-MS)分析根系分泌物中的成分;通过菌丝生长速率法测定三七根系分泌物中部分糖类、氨基酸类和长链有机酸对三七根腐病原菌生长的影响。
【结果】三七根系能显著促进茄腐镰刀菌和恶疫霉菌孢子萌发且对芽管生长方向具有明显的吸引作用。
三七根系分泌物中主要含有酸类、醇类、糖类、氨基酸类、胺类、嘌呤嘧啶类、酯类、酮类和酚类等9类物质。
其中酸类、醇类、糖类和氨基酸类是根系分泌物中的主要成分,且长链有機酸类(苯甲酸、邻苯二甲酸和月桂酸)对根腐病原菌具有低促高抑效应,糖类(纤维二糖和麦芽三糖)和天冬氨酸可作为唯一碳源和氮源促进病原菌菌丝生长,且纤维二糖和麦芽三糖能促进根腐病原菌孢子萌发,对镰刀菌孢子萌发的芽管具有趋化作用。
【结论】三七根系分泌物对根腐病原菌的生长具有促进作用,且对芽管的生长方向具有吸引作用。
关键词: GC-TOF-MS;趋化;孢子萌发;镰刀菌;根腐病中图分类号: S435.672 文献标志码: A 文章编号:2095-1191(2020)12-2952-10Abstract:【Objective】The effect of Panax notoginseng(Bark)F. H. Chen root exudates on root rot pathogens was studied to provide a theoretical basis for analyzing the mechanism of interaction between plant and soil-borne pathogens mediated by root exudates. 【Method】The effects of root of P. notoginseng on spore germination and germ tube growth direction of Fusarium solani and Phytophthora cactorum were studied by root-spore interaction method. The root exudates were collected and the components of root exudates of P. notoginseng were analyzed by gas chromatography-time-of-flight mass spectrometer(GC-TOF-MS), and then determined the effects of some metabolites(sugars, amino acids and long-chain organic acids) in root exudates on the growth of root rot pathogens by mycelium growth rate method. 【Result】The results showed that the spore germination rate of F. solani and P. cactorum was significantly increased around the root and the germ tubes had chemotaxis activity towards roots of P. notoginseng. Root exudates of P. notoginseng contained nine kinds of substances, including acids, alcohols, sugars, amino acids, amines, purines, esters, ketones and phenols. Among them, acids, alcohols, sugars and amino acids were the main components in root exudates of P. notoginseng. Long-chain organic acids (benzoic acid, pathalic acid, palmitic acid and lauric acid) could promote and inhibite the growth of root rot pathogen according to the condition. Sugars(cellobiose and maltotriose) and aspartic acid could promote the mycelium growth of pathogen as the sole carbon source and notrogen source respectively. Cellobiose and maltotriose also promoted the germination of root rot pathogen spores and had a chemotaxis on the growth of germ tube of Fusarium. 【Conclusion】Root exudates of P. notoginseng can promote the growth of root rot pathogens, and has attraction effect on the growth direction of germ tube.Key words: GC-TOF-MS; chemotaxis; spore germination; Solani; root rotFoundation item: National Natural Science Foundation of China(31772404, 31660605); Young and Middle-aged Academic and Technical Talent Reserve Program in Yunnan(202005AC160045); Yunnan Academician Workstation of Chinese Academy of Engineering (2018IC063)0 引言【研究意义】三七[Panax notoginseng(Burk.) F. H. Chen]为五加科人参属植物,是我国独具特色的大宗药用作物,具有散瘀止血、消肿定痛等功效,对心脑血管系统疾病具有显著疗效(张玉军,2009)。