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牙龈卟啉单胞菌菌毛的生物学特性及毒力王琳;张建钢;肖水清【摘要】Fimbriae(Fim), one of the important pathogenic factors of Porphyromonasgingivalis(P.gingivalis), plays an important role in the attachment to host cells . Filamentous protrusions on the surface of the cell increase the attachment t o the cell and salivary protein, extracellular matrix, eukaryotic cells, and simila r other species of bacteria. These protrusions also participate in the formation of biofilms. FimA protein, the main subunit of the fimbriae of P.gingivalis, is n ot only related to the invasion into epithelial cells and connective tissues in pe riodontal tissue but also has a certain destructive effect on the alveolar bone. This paper describes the biological characteristics and virulence of P.gingivali s fimbriae.%菌毛（Fim）是牙龈卟啉单胞菌的重要致病因子之一，对宿主细胞的黏附发挥着重要作用。
Fim是细胞表面的丝状突起，增强了细胞对唾液蛋白、细胞外基质和真核细胞以及同类或其他种属细菌的黏附性并参与生物膜的形成。
•306•口腔医学2019年4月第39卷第4期牙龈吓咻单胞菌呦P基因缺失株和回补株的构建和鉴定何露，王宏媛，张茹，侯本祥，李红［摘要］目的构建并鉴定牙龈吓咻单胞菌(Porphyromonas gingivalis,P.g)W83的丝氨酸蛋白酶(caseinolytic protease,Clp)基因缺失株和回补株，为探索MpP基因在P.g致病过程中的作用和机制奠定基础方法设计基因的引物对,PCR扩增其上下游同源片段，克隆入质粒|)UC18中,插入红霉素抗性基因ermB作为筛选标记，构建重组质粒，电转化入P.g W83构建缺失株；PCR扩增也沪基因片段与重组质粒pUClS-Ac/pP-er/nB连接，电转化入P.g W83缺失株中构建回补株采用PCR和酶切电泳鉴定序列的正确性，利用抗性培养基筛选高表达菌株.结果旳沪基因克隆成功并顺利导入质粒P UC18中,P.g W83的也沪基因缺失株和回补株构建成功，并能在体外稳定传代一结论本研究运用同源重组技术构建了P.g W83的cQP基因缺失株，并建立了以plICIS质粒为载体的也P基因回补方法，为进一步研究c"沪基因在P.g致病过程中的作用打下了基础［关键词］clpP基因；牙龈吓咻单胞菌；缺失重组株；回补重组株［中图分类号］R780.2［文献标识码］A［文章编号］1003-9872(2019)04-0306-05［doi］10.13591/j.c nki.kqyx.2019.04.004Construction and identification of the clpP deletion strains and complemented strains in Porphyromonas gingivalisHE Lu,WANG Hongyuan,ZHANG Ru,HOU Benxiang,LI Hong.(Department of Endodontics,School of Stomatology,Capital Medical University,Beijing100050,China)Abstract:Objective To constmct and identify caseinolytic protease(Clp)gene deletion stiains and complemented strains of Por-phyromonas gingivalis(P.g)W83in order to make a foundation for exploring the role and mechanism of clpP gene in the pathogenesis of P.g W83.Methods The primer pair of clpP gene was designed and its upstream and downstream homologous fragments were amplified by PCR,which were cloned into plasmid pUC18.And the erythromycin resistance gene erm/3from plasmid pMG36e was inserted as a screening marker to construct a recombinant plasmid,which was electrotransfered into P.g W83to get the corresponding clpP deficient recoml)inant strain.The clpP gene fragment was amplified by PCR and ligated with the recombinant plasmid pUC1S-SclpP-errnB and electrotransfered into the recombinant strain of P.g so as to constmct the corresponding complemented recombinant strain.PCR and e-lectrophoresis were used to identify the correctness of the sequence,and the high-expression strain was screened and selected using a resistant medium.Results The clpP gene fragment was successfully cloned and introduced into the plasmid vector pUC18.The clpP gene deletion strain and the complemented strain of P.g W83were successfully constructed and could have a stable passage in vitro. Conclusion In this study,the clpP gene deletion strain of P.g W83is constructed using homologous recombination technology,and the method of using pUC18plasmid as a vector to construct the complemented strains is also established,making a foundation for the fm1her study on the role of clpP gene in the pathogenesis of P.g.Key words:clpP；Porphyromonas gingivalis；deletion strain；complemented strainStomatology,2019,39(4)：306-310牙龈吓咻单胞菌(Porphyromonas gingivalis,P.g)是公认的牙周病和牙髓根尖周病的重要致病菌之-1.它与许多全身性疾病(动脉粥样硬化和类风湿性关节炎等)也密切相关〔I。
