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Effects of Shenshao Decoction on the inflammatory response in the aorta of a rat atherosclerotic

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• 347 •Chin J Integr Med 2013 May;19(5):347-352Shenshao Decoction (参芍汤) is a traditional herbal medicine composed of Radix Salviae Miltiorrhizae, Astragalus Membranaceus, Radix

Paeoniae Rubra, Radix Angelicae Sinensis, Rhizoma

Chuanxiong, Semen Persicae, Flos Carthami,

Pheretima Aspergillum, Hirudo, and Rhizoma Pinelliae. Shenshao Decoction was developed as an oral liquid a decade ago and has been registered with the State Food and Drug Administration of Hebei province (Z20050957, China). We have previously shown that Shenshao Decoction can effectively prevent and suppress left ventricular remodeling after acute myocardial infarction in rats.(1) We have also demonstrated that Shenshao Decoction has cardioprotective effects after acute ischemia-reperfusion in rats.(2,3) While similar results have also been observed in humans,(4) the underlying mechanisms remain unclear. The inflammatory response is a basic pathological mechanism that stimulates the formation of atherosclerotic plaque. While macrophages are known for their contribution to the inflammatory element of atherosclerosis, the role of the T lymphocyte is relatively understudied. The newly discovered interleukin (IL)-17A-producing T cell subset, known as the Th17 cell, plays a major role in chronic inflammatory and autoimmune diseases, such as rheumatoid arthritis, infl ammatory bowel disease, and multiple sclerosis. Th17 cells have also been shown to contribute to atherogenesis.(5) Recently, the Th17-

derived cytokine, IL-17A, was shown to be involved in the pro-infl ammatory response of vascular smooth muscle cells. The IL-23 and IL-17A axis is emerging as a critical regulatory system that bridges the innate and adaptive arms of the immune system. IL-1β plays a crucial role in early Th17 cell differentiation,(6) and

ORIGINAL ARTICLEEffects of Shenshao Decoction (参芍汤) on the Infl ammatory Response in the Aorta of A Rat Atherosclerotic Model

XUE Zhong-wen (薛忠文)1, SHANG Xiao-ming (尚小明)1, LV Song-hui (吕松桧)3, XU Hong (徐 洪)4, ZHANG Qi (张 琦)2, and WANG Chen (王 晨)2

©The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg 20131. Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei Province (050000), China; 2. Department of Cardiology, the Tangshan Worker's Hospital of Hebei Medical University, Tangshan, Hebei Province (063000), China; 3. Department of Obstetrics, Beijing Fangshan District Maternal and Child Health Hospital, Beijing (102488), China; 4. Medical Research Center, Hebei United University, Tangshan, Hebei Province (063000), ChinaCorrespondence to: Dr. SHANG Xiao-ming, Tel: 86-315-2814801, Fax: 86-315-3722242, E-mail: shangxiaoming0616@163.comDOI: 10.1007/s11655-013-1457-z

ABSTRACT Objective: To investigate the effect of Shenshao Decoction (参芍汤) on the infl ammatory status in the aorta in a rat model of atherosclerosis. Methods: Forty Sprague-Dawley rats were randomly divided into fi ve groups, 8 rats in each group: control untreated group, atherosclerosis group, atherosclerosis with Shenshao Decoction (low dose) group, atherosclerosis with Shenshao Decoction (high dose) group, atherosclerosis with simvastatin group. To stimulate atherosclerosis, the rats were fed vitamin D3 and a high-cholesterol diet. Four weeks later, treatments were maintained for eight weeks. Morphology changes were investigated by hematoxylin and eosin staining. Serum levels of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) were obtained by enzymatic assays with use of an automated biochemical analyzer. The expression of malondialdehyde (MDA), glutathione peroxidase (GSH-PX) were detected by enzyme-linked immunosorbent assay. The expression levels of interleukin (IL)-1β, IL-17A, and IL-23 were detected by immunoblotting. Results: Shenshao Decoction treatment decreased TC, TG, LDL-C and MDA and increased GSH-PX levels (P<0.01). Compared with the control group, IL-1β, IL-17A, and IL-23 were lower in the high and low dose Shenshao Decoction groups and the simvastatin group (P<0.01). Conclusion: Shenshao Decoction could attenuate the progression of aortal atherosclerotic plaques by inhibiting the infl ammatory response in a rat atherosclerotic model.KEYWORDS Shenshao Decoction, atherosclerosis, malondialdehyde, glutathione peroxidase

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稳斑汤对动脉粥样硬化小鼠血脂代谢的影响

稳斑汤对动脉粥样硬化小鼠血脂代谢的影响

稳斑汤对动脉粥样硬化小鼠血脂代谢的影响发布时间：2021-12-03T02:05:24.557Z 来源：《教学与研究》2021年20期作者：赵兵[导读] 目的：通过观察稳斑汤对动脉粥样硬化模型小鼠血脂的影响，进而评估该药物对颈动脉粥样硬化的改善作用。

赵兵辽宁中医药大学辽宁沈阳110032摘要：目的：通过观察稳斑汤对动脉粥样硬化模型小鼠血脂的影响，进而评估该药物对颈动脉粥样硬化的改善作用。

方法：将60只ApoE基因敲除小鼠高脂喂养13周建立动脉粥样硬化模型，然后按随机数字表法分成组，分别为模型组，稳斑汤低、中、高剂量组和阿托伐他汀组，另取10只C57BL/6J小鼠设为空白对照组，空白对照组和模型组每日给予适量0.9%生理盐水灌胃，稳斑汤低、中、高剂量组分别给予6.175、12.35、24.7g/(kg.d)灌胃，托伐他汀组小鼠予以阿托伐他汀钙片 25.4mg/(kg.d)灌胃，13周后处死小鼠收集血液，检测各组小鼠血脂含量。

结果：与空白对照组相比，模型组血清TC、TG、LDL-C表达水平均升高（P<0.05），HDL-C表达水平降低 (P<0.05)，与模型组相比，稳斑汤各剂量组和阿托伐他汀血清TC、TG、LDL-C表达水平均降低（P<0.05）,HDL-C表达水平升高（P<0.05）。

结论：稳斑汤可在一定程度上改善动脉粥样硬化小鼠血脂水平，稳定斑块，从而起到对血管的保护作用。

关键词：稳斑汤；动脉粥样硬化；血脂；小鼠Effect of Wenban Decoction on Blood Lipid Metabolism in Mice with Atherosclerosis Zhao BingLiaoning University of Traditional Chinese Medicine 110032Abstract: Objective: To evaluate the effect of the drug on carotid atherosclerosis by observing the effect of Wenban Decoction on blood lipids in atherosclerosis model mice. Methods: 60 ApoE knockout mice were fed high-fat for 13 weeks to establish atherosclerosis models, and then divided into groups according to the random number table method, namely model group, Wenban Decoction low, medium and high dose group and Atuo In the Vastatin group, another 10 C57BL/6J mice were taken as the blank control group. The blank control group and the model group were given an appropriate amount of 0.9% normal saline daily. 12.35, 24.7g/(kg.d) gavage, mice in the torvastatin group were gavaged with atorvastatin calcium tablets 25.4mg/(kg.d), 13 weeks later, the mice were sacrificed to collect blood, and the mice in each group were tested. Rat blood lipid content. Results: Compared with the blank control group, the expression level of serum TC, TG, LDL-C in the model group increased (P<0.05), and the expression level of HDL-C decreased (P<0.05). Compared with the model group, the plaque was stable The expression levels of TC, TG and LDL-C in serum of each dose group of decoction and atorvastatin decreased (P<0.05), and the expression level of HDL-C increased (P<0.05). Conclusion: Wenban Decoction can improve the blood lipid level of atherosclerotic mice to a certain extent, stabilize the plaque, and protect the blood vessels. Keywords: Wenban Decoction; Atherosclerosis; Blood Lipid; Mice；动脉粥样硬化（AS）是冠心病、脑卒中等心脑血管疾病的主要病理学基础，并且呈现逐年上升且年轻化的趋势。

Stanford B型主动脉夹层术前发生全身炎症反应综合征的多因素Logistic回归分析要点

Stanford B型主动脉夹层术前发生全身炎症反应综合征的多因素Logistic回归分析要点

验，两组独立样本单向有序联表资料采用趋势Ｙ２检
验。多因素分析采用逐步Ｌｏｇｉｓｔｉｃ回归。Ｐ＜０．０５认 为差异有统计学意义。 ２结果 ２．１一般情况 本组主动脉夹层术前资料显示，患者术前合并高 血压病史１０８例，占７１．５２％。主动脉夹层出现脏器 灌注不良综合征２３例，累及率为１５．２３％。
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ｓｙｎｄｒｏｍｅ；Ｒｅｇｒｅｓｓｉｏｎ ａｎａｌｙｓｉｓ
主动脉夹层（Ａｏｒｔｉｃ ｄｉｓｓｅｃｔｉｏｎ，ＡＤ）是主动脉内
ＤＯＩ：１０．３７６０／ｃｍａ．ｊ．ｉｓｓｎ．１６７３・４２０３．２０１６．０６．００５
作者单位：８３０００１乌鲁木齐，新疆维吾尔自治区人民医院ｌ临床检 验中心（庞秀慧），麻醉科（何亮、张立），心外科（买买提艾力・艾则 孜、阿依别克・乃比、郭盛、郭永忠、刘筠、张总刚、唐和年）；１０００３７北 京，中国医学科学院阜外医院血管外科中心（杜宇奎、杨秀滨） 通信作者：张总刚，Ｅｍａｉｌ：ｚｈａｎｇｚｏｎｇｇａｎ９６２１９＠１６３．ｔｏｍ
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二陈汤通过调控脾脏铁转运能力改善非酒精性脂肪性肝病小鼠的铁代谢

二陈汤通过调控脾脏铁转运能力改善非酒精性脂肪性肝病小鼠的铁代谢

Erchen Decoction improves iron homeostasis in mice with non-alcoholic fatty liver disease by regulating iron transport capacity in the spleenDENG Guanghui 1,2,JIA Hui 1,LI Yunjia 1,LI Junjie 1,WU Chaofeng 1,SHI Hao 1,QIN Mengchen 1,ZHAO Jiamin 1,LIU Chang 1,LIAO Yuxin 1,GAO Lei 1,21School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;2Department of Gastroenterology,Integrated Hospital of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510315,China摘要：目的探讨二陈汤对非酒精性脂肪性肝病铁稳态的影响以及调控脾脏细胞铁离子转运能力的机制。

方法将36只雄性C57BL/6J 小鼠，随机分为对照组、模型组、二陈汤低剂量组（7.5g/kg ）、二陈汤中剂量组（15g/kg ）、二陈汤高剂量组（30g/kg ）、多烯磷脂酰胆碱组（9.12mg/kg ），6只/组。

对照组给予低脂低糖饮食，其余组给予高脂饲料饲养12周。

药物组于第7周开始灌胃给药，其余组灌胃等体积饮用水，3次/周。

采用HPLC-MS 检测二陈汤的活性成份；HE 染色和尼罗红染色评估肝脏脂质累积情况；普鲁士蓝染色观察脾脏铁含量；蛋白印迹法、免疫组织化学法以及免疫荧光染色法检测铁转运蛋白（Fpn1）、转铁蛋白受体（TfR ）、前列腺六跨膜上皮抗原3（Steap3）、血红素加氧酶1（HO-1）、Ter-119、CD163和CD68的表达。

活血潜阳祛痰方对肥胖高血压大鼠心肌氧化应激及炎症的影响

活血潜阳祛痰方对肥胖高血压大鼠心肌氧化应激及炎症的影响

-研#报告-活血潜阳祛痰方对肥胖高血压大鼠心肌氧化 应激及炎症的影响1芦 波谢君 符德玉2陈晓* 赵铭一桂明泰 周训杰 姚 磊 李建华*基金项目：国家自然科学基金青年科学项目(81883062);国家自然科学基金面上项目(81774111);上海 市科学技术委员会科研计划项目(19401970400);上海市进一步加快中医药事业发展三年行动计划 (201年-2022年)项目2通信作者(电子邮箱：fdy65@ )(上海中医药大学附属岳阳中西医结合医院，上海200437)中图分类号:R285.5文献标志码:A 文章编号：1004-745X(2021)03-0411-05doi ：10.3969/j.issn 」004-745X.2021.03.009【摘要】目的观察活血潜阳祛痰方对肥胖高血压(OBH )大鼠心肌氧化应激及炎症的影响。

方法自发性高 血压大鼠(SHR )喂养高脂饮食制备OBH 大鼠，随机分为OBH 组、中药低剂量组、中药高剂量组和j 沙坦组。

以Wistar 大鼠(WKY )和SHR 为对照。

治疗1周后测量体质量、左心室质量，观察心肌病理,检测心脏超声、血 清和心肌中血管紧张素K (AngK )水平、心肌中丙二醛(MDA )、*超氧化物歧化酶(Mn-SOD )、NADPH 氧化酶4(NOX4)、核因子k B(NF-k B)、细胞外信号调节激酶l/2(ERKl/2)和p-ERKl/2的水平，对相关数据统计分析。

结果与WKY 组比较，OBH 组左室质量指数(LVMI )和矫正的左心室质量(LVM-cor )均显著升高(！<0.35),血清和心肌Ann K 水平明显升高(P < 0.05),心肌中NOX4、NF-k B p65蛋白表达和ERK1/2磷酸化比值明显升高(！<0.05)。

中药高剂量组的心脏质量指数、LVM-cor 和血清Ann K 水平较模型组降低(！<0.05)。