静磁场对口腔组织的生物学效应研究

静磁场对成骨细胞增殖及细胞周期的影响
仇丽鸿;汤旭娜;钟鸣;王兆元
【期刊名称】《上海口腔医学》
【年(卷),期】2004(13)5
【摘要】体外培养Wister大鼠颅骨成骨细胞,分别置于0Gs、400Gs、620Gs、830Gs、1080Gs不同磁场强度的静磁场中作用24h、48h和72h。

用噻唑兰(MTT)法检测成骨细胞的增殖情况,流式细胞仪测定不同磁场强度下细胞所处的周期。

结果显示,各组均出现不同程度的促增殖作用,尤以400Gs和620Gs组作用72h时显著;620Gs磁场治疗组S期、G2/M期细胞百分比显著增加。

推测一定磁场强度可促使静止态的G1期细胞活跃,向S期转变,细胞增殖加速。

【总页数】3页(P469-471)
【关键词】成骨细胞;增殖;细胞周期;静止态;治疗组;流式细胞仪;MTT;情况;转变;百分比
【作者】仇丽鸿;汤旭娜;钟鸣;王兆元
【作者单位】中国医科大学口腔医学院口腔内科教研室
【正文语种】中文
【中图分类】R446;R329
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1.磁性附着体模拟静磁场对成骨细胞增殖活性和周期分布及凋亡率的影响 [J], 赵煜;李冰雁;巢永烈;杜莉
2.静磁场作用下溶胶-凝胶生物活性玻璃对成骨细胞增殖性的影响 [J], 吴芳蕾;李蜀光;陈晓峰;王丽艳
3.丹参酮ⅡA对成骨细胞MC3T3-E1增殖、凋亡以及细胞周期分布的影响 [J],
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5.流体剪切应力对成骨细胞增殖效应及细胞周期的影响研究 [J], 任婕
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牙周炎微环境下静态牵张力对牙周膜干细胞中炎性细胞因子分泌的影响蔡东轩1’2，孙唯夫1'3，金作林1'3，刘佳1[摘要]目的探究不同大小静态牵张力（static mechanical strain,SMS)对正常来源的牙周膜十细胞（periodon丨aMigament stem cells obtained {r(mi healthy 丨)門.i()(J(>ntal tissues，HI)I)ISCs)和牙周病组织来源obtained from periodontal tissues o丨periodontitis patients,PPDLSCs)炎性细胞因子表达的影响方法以HPDLSCs PTOLSC 为对象构建细胞体外培养-力学刺激模型，采用Flexcell牵张力加载装置，分别给予静态牵张力（频率为0.1 Hz，力值设定为 6%、8%、10%、12%和14%)刺激12 h，并设立正常对照组。

采用Elisa检测SMS加载后HPDLSCs和PPDLSCs中炎性细胞因子 IL-i p、TNF-a、lL-6和丨L-8分泌水平结果未加力情况下PPDLSCs中IL-i p、TNF-a、lL-6和丨L-8分泌水平均高于HPDLSCs (P<0.05)，加载SMS后PPDLSCs中I L-ip和T N F-a的表达水平略有升高（P<0.05)，而IL-6和IL-8的表达水平则大幅提升，且在一定的范围内与SMS的大小呈正相关t结论牙周炎微环境导致PDLSCs中炎性细胞因子的表达情况增强且不同的炎 性细胞因子对SMS的敏感性有差异，其中IL-6和IL-8表达量明显升高，提示其可能参与了牙周炎微环境下PDLSCs对SMS的反应。

[关键词]牙周炎微环境;静态牵张力；牙周膜干细胞；炎性细胞因子[中图分类号]R781.42 [文献标识码]A[文章编号]1003-9872(2020)12-1079-05[doi]10.13591 /ki.kqyx.2020.12.004The effect of static mechanical strain on the inflaiiiinatory cytokine reactions of IIPDLSCs in periodontitis microenvironment CA1 Dongxuan , SUN Weifu , JIJS Zuolin , LIU Jia. ( Stale Key Laboratory of Military Stomatology & National Clinical Research Center f or Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Xi'an 710045, China)Abstract ：Objective To investigate the effect of different magnitudes of SMS on the inflammatory cytokine reactions of HPDLSCs and PPDLSCs. Methods In vitro culture-tensile stimulation models of HPDLSCs and l)PDLSCs were established using a Flexcell Ten­sion Unit. SMS was applied on HPDLSCs and FPDIJSCs (the load was set for 6%, 8%, 10%, 12%, 14%, with frequency of 0.1 Hz) for 12 hours. At the same time,the nonnal control group was established. The secretions of inflammatory cytokines IL-6, IL-8, IL-ip and TNF-a were examined by Elisa. Results The secretion of IL-6, IL-8, IL-ip and TNF-a were higher in PPDLSCs than in HPDLSCs without SMS (P<0.05). When SMS was loaded, the expressions of IL-ip and TNF-a increased a little in PPDI^Cs (/)<0.05). How­ever, the expression of IL-6 and IL-8 was up-regulated to n considerable high level and had a positive correlation with the magnitude of SMS in a certain range in PPDLSCs. Conclusion The periodontitis microenvironment inc reases the secretion of inflamniatorv cytokines of PDLiiCs. Under SMS, different inflammatory cytokines are up-regiilated by different extents, among which IL-6 and IL-8 increases considerably, which indicates that IL-6 and IL-8 are involved in the reaction of PDLSCs to SMS in periodontitis microenvironment. Key words：periodontitis micToenvironment；static mechanical strain；jjeriodontal ligament stem cells；inflammatory cytokineStomatology,2020,40( 12) ： 1079-1083牙周病是最常见的口腔疾病之一，在我国大约 有90%的人患有牙周疾病，其中能造成深部牙周组 织结构破坏的牙周炎发病率大于40%，有活动性牙 周炎的人群比例也在逐年上升[1]。