下载文档原格式
pAcGP67B BACULOVIRUS TRANSFER VECTORProduct InformationCatalog Number:554757Contents: 5 µg in 50 µlDescriptionThe acidic glycoprotein gp67 (syn.: gp64) is the most abundant envelope surface glycoprotein of the Autographa californica nuclear polyhedrosis virus (AcNPV baculovirus), and is essential for the entry of baculovirus particles into susceptible insect cells.1Since large amounts of this protein are secreted and anchored to the virus peplomer, its gene contains one of the most effective baculovirus-encoded signal sequences for protein secretion.2 Therefore, we have constructed baculovirus transfer vectors (pAcGP67A, B, C) that contain the gp67 signal sequence in front of a multiple cloning site (5’-BamH I, Sma I/Xma I, Xba I or Nco I, EcoR I, Not I, Eag I, Pst I and Bgl II-3’). A gene of choice can be inserted in one of these cloning sites and the protein of interest will be expressed as a gp67 signal peptide fusion protein under the control of the strong baculovirus polyhedrin promoter. This strategy allows the forced secretion of otherwise non-secreted recombinant proteins which may be easily purified when serum-free insect culture medium, BD BaculoGold™ Max-XP Insect Cell Medium (Cat. No. 551411) is used. The transfer vector(s) should be used in conjunction with BD BaculoGold™ DNA (Cat. No. 554739).Preparation and StorageThe vectors were purified using a silicon bead matrix and dissolved in TE buffer (10 mM Tris-HCl, pH 7.5; 1 mM EDTA).Each vector should be stable for at least 2 years when stored at –20°C.HandlingAmplify the plasmid DNA in E. coli strains (DH5_, HB101 or any other suitable strain) under ampicillin selection. Insert your gene of interest into a suitable restriction site that is in frame with the gp67 signal sequence. Do a co-transfection of the recombinant plasmid and BD BaculoGold™ Linearized Baculovirus DNA (Cat. No. 554739) using a susceptible insect cell line (e.g., Sf9 or Sf21) and identify recombinant viruses expressing your protein.References1.Whitford M., Stewart S., Kuzio J., Faulkner P. 1989. J. Virol. 63:1393-1399.2.Stewart L.M.D, Hirst M., Ferber M.L., Merryweather A.T., Cayley PJ, Possee RD. 1991. Nature. 352:85-88.3.Sambrook, Fritsch, and Maniatis. 1989. Molecular Cloning, 2nd edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.B。
pCoBlast编号 载体名称北京华越洋生物KCVECT105 pCoBlastpCoBlast载体基本信息载体名称: pCoBlast质粒类型: 昆虫表达载体；筛选载体克隆方法: --启动子: copia载体大小: 3907 bp5' 测序引物及序列: --3' 测序引物及序列: --载体标签: 无载体抗性: 氨苄青霉素真核筛选标记: Blasticidin克隆菌株: TOP10；DH5-T1R宿主细胞（系）: 果蝇细胞系S2备注: pCoBlast载体与昆虫表达载体共转染，建立稳表达细胞系。
瞬表达/稳表达: --组成型/诱导型: 组成型病毒/非病毒: 非病毒pCoBlast载体质粒图谱和多克隆位点信息pCoBlast载体简介pCoBlast is a 3907 bp selection vector that can be cotransfected with the expression vector of choice to to create stable cell lines in Drosophila. It contains the Streptomyces griseochromogenes bsd gene under control of the Drosophila copia promoter to confer resistance to blasticidin in S2 cells.引用及参考文献A novel allelic variant of the human TSG-6 gene encoding an amino acid difference in the CUB module. Chromosomal localization, frequency analysis, modeling, and expression.Nentwich Hilke A; Mustafa Zehra; Rugg Marilyn S; Marsden Brian D; Cordell Martin R; Mahoney David J; Jenkins Suzanne C; Dowling Barbara; Fries Erik; Milner Caroline M; Loughlin John; Day Anthony J;J Biol Chem (2002) 277:15354-15416Product usage: The full-length coding sequence of human TSG-6 (A431 allele encoding Gln at amino acid 144), including the signal sequence and stop codon,was excised from the pAcCL29-1 vector by digestion with KpnI/XbaI and cloned into the corresponding sites in the Drosophila Expression SystempCoBlast载体序列TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCT GTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGG CTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGAT GCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATC GGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTA ACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCG ACTCTAGAGGATCCGGTGCGTGGTGGTTTCATGCTTCTGGGAACGGCAAATGGGTTTAGGATTGGGAACC CCTCATCATCTGTTGGAATATACTATTCAACCTACAAAAATAACGTTAAACAACACTACTTTATATTTGA TATGAATGGCCACACCTTTTATGCCATAAAACATATTGTAAGAGAATACCACTCTTTTTATTCCTTCTTT CCTTCTTGTACGTTTTTTGCTGTGAGTAGGTCGTGGTGCTGGTGTTGCAGTTGAAATAACTTAAAATATA AATCATAAAACTCAAACATAAACTTGACTATTTATTTATTTATTAAGAAAGGAAATATAAATTATAAATT ACAACAGGTTCCCTTTATGCGAAGGGATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAG AGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGC GACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGCGCAGAACTCGTGGTGC TGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGG CATCTTGAGCCCCTGCGGACGGTGCCGACAGGTTCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTG AAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGG GCTAACCCTTATACGCAAGGGAGTAGATGCCGACCGAACAAGAGCTGATTTCGAGAACGCCTCAGCCAGC AACTCGCGCGAGCCTAGCAAGGCAAATGCGAGAGAACGGCCTTACGCTTGGTGGCACAGTTCTCGTCCAC AGTTCGCTAAGCTCGCTCGGCTGGGTCGCGGGAGGGCCGGTCGCAGTGATTCAGGCCCTTCTGGATTGTG TTGGTCCCCAGGGCACGATTGTCATGCCCACGCACTCGGGTGATCTGACTGATCCCGCAGATTGGAGATC GCCGCCCGTGCCTGCCGATTGGGTGCAGATCAGCCTCGAGGCCAGCTAGCTTGAACTTGTTTATTGCAGC TTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCT AGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCGGGTACCGAGCTCGAATTCGTA ATCATGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAA GCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCC CGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT TTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAG CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACAT GTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTC CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCG GTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAA CAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTAC ACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG AAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAA GTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC ACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACG ATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAG ATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTC CATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTT GTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCC AACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGAT CGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACT GTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTA TGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAA AGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGT TCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAG CAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACT CTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGT ATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAApCoBlast其他昆虫表达载体：pMT/BioEase-DEST pVL1392 pVL1393 pXINSECT-DEST39 pXINSECT-DEST38 pBacPAK9 pBacPAK8 pIB/V5-His-TOPO pIEX/Bac-1 pFastBacHT B pFastBacHT A pFastBac1pMT/V5-His-TOPO pMT/BiP/V5-His A pCoHygro pAc5.1/V5-His C pAc5.1-V5-His A pAc5/V5-HisA pAc5-V5-HisB pAc5-V5-HisC pCoBlast pAc5.1/V5-His B pIZ/V5-His pIB/V5-HispIZT/V5-His pMIB/V5-His C pMIB/V5-His A pMT/BiP/V5-His C pMT/BiP/V5-His B pIEx/Bac-4 pIEx/Bac-3 pIEXBac-c-EGFP-3 pIEXBac-c-EGFP-1 pIEXBac-c-EGFP-4 pAc5.1B-EGFP pBlueBacHis2 A pBlueBacHis2 C pBlueBacHis2 B pFastBacHT C pMT-Bip-V5-HisA pFBDM pUCDM pFastBac Dual pMIB/V5-His BpMT/V5-His C pMT/V5-His B pMT/V5-His A pAcGP67ApAc5.1b pAc5.1a pVL1392-XyIE control vector pFastBac-c-His-TEV pFastBac-N-GST-TEV pFastBacI-Gus pFastBacHT-CAT pBADZ-His6Cre。
pXINSECT-DEST38编号 载体名称北京华越洋生物KCVECT101 pXINSECT-‐DEST38pXINSECT-‐DEST38载体基本信息载体名称: pXINSECT-DEST38质粒类型: 昆虫表达载体；Gateway载体克隆方法: Gateway启动子: Actin载体大小: 12419 kb5' 测序引物及序列: --3' 测序引物及序列: --载体标签: 无标签载体抗性: 氨苄青霉素、氯霉素（仅空载体）真核筛选标记: 无克隆菌株: DB3.1宿主细胞（系）: High Five（推荐）等细胞系备注: pXINSECT-DEST38载体与pBmA-neo载体共转染，可以建立稳表达细胞系；应用Gateway技术，可快速高效将目的基因片段（尤其适用于目的基因片段>1kb，用传统的连接酶法效率低的）插入到载体中；瞬表达/稳表达: 瞬表达组成型/诱导型: 组成型病毒/非病毒: 非病毒pXINSECT-‐DEST38载体质粒图谱和多克隆位点信息pXINSECT-‐DEST38载体简介Using t he E xpress I nsect K it o r t he E xpress I nsect V ector S et t o a chieveprotein e xpression in i nsect c ells h as t he f ollowing a dvantages:Non-‐lytic, c ontinuous p rotein e xpressionMinimal d egradation o f y our p rotein o f i nterest a s c ompared t o v iralexpression s ystems Higher protein expression level achieved than other similar non-‐viral insectexpression systemsChoice o f t ransient o r s table p rotein e xpressionAdapted for use with the Gateway Technology or traditional restrictionenzyme-‐mediated c loningpXINSECT-‐DEST38载体特征：pXINSECT-‐DEST38 a nd p XINSECT-‐DEST39 h ave t he f ollowing f eatures:The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression o f t he g ene o f i nterest (Johnson e t a l., 1992)The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin p romoter (Lu e t a l., 1997)A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your geneof i nterest (Lu e t a l., 1996)N-‐terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein o f i nterest u sing p XINSECT-‐DEST39Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest f rom a n e ntry c loneChloramphenicol r esistance g ene l ocated b etween t he t wo a ttR s ites f or c ounterselection The c cdB g ene l ocated b etween t he t wo a ttR s ites f or n egative s electionThe actin polyadenylation signal for efficient transcription termination and polyadenylation o f m RNAAmpicillin r esistance g ene f or s election o f t ransformants i n E. c oliThe p UC o rigin f or h igh c opy r eplication a nd m aintenance o f t he p lasmid i n E. c olipXINSECT-‐DEST38载体序列: ATGTATAATTAATTAAATTCAAATTTTTTATATTTACAATTTAGTTTTTGTTCCGCAAACGTTATAGCGT CGGACAACGGAACCAGACCCTGTAATATTAAAGCTAACAATTTTAACAAATTATTGTGCAATGTAGTGCT CTCTCTTCGGTTCACTTTACTGATTACAAACATGTGATGCTTAAATCTATTATATTTTTGAATTACTTGA CTAGCGTCTACATCTTTAATCTCGCCAGAAATCCAATAAAACTCTTCGTTTTTCTTAGCTATAGTCAACC GCTCTTCGTTTTTGAAAGACAATACTATAAAATTGTGACCTTTTACATTATCCACATTCTGAGTCAAATA CTGTTCGACAATGTGCATGCTGCCGTCCTCCTTCTTAACCTTTTTTAAATTTTCAGCGTTATTATTACTC GCAATATTGTCATGATATTTATAATTATTAAACAAAAGATTAGCGACACTACTGTATTTGTACGTGAGCG TACTTTTTTTGTTAACAATTAAATTTAAATTGTCCACCACATATTTGTTTGGGGGATTGTCGGGAAACTT TACACTTTCCGAATACTTTAATATTTGACTCACATACGGCGATACAAAAAAATTATTAGATGCAGTCTCA ATTTCATTACTCTCTTTACGACTAAGCATAATAGGCAAAGTAAATAAATTTTTATCTTGATACATTTCGT ACAACTTGCTCAAAAGAAACCCACACTTTCTTTCGCCCAACGATTGTAACAAAGTCACAAATGTGGTTTG CGCGTAATACATATCTAAATTAAAATATGAAGTCAGAGCAGCTTTAAACGTGTGATGCACATCGACAAAG TGGCATTTTTTACAATTTTGTGCAGCCGTCTCGTCGTTGCACACATCTTGAGAATGAGGAATTTCTATGC CGGTTTCTTTAACCAAATTGTACGAGATCATAAATCTAATTTTATCAAAAGTTACCACAAACACGCGATT ATCTACCATGTAATAGTTGTTTGTATATTCGTACACCACATTGCTCACGTACTTGGCAAATATAATTTCA AACGGCTTTACTTCACTTTTTTTAACCACAAACATGTAATAACCAGTTTCGGACATATGGTCGGAGAACC TATTGGAATTGTAGTCGTTGTCGTCGAAACGCATCAAATACGGCGCAAAATCATTAGTAAAATAATGCGT AATTTCTTGAGTTGAAGCAACCGTGCAAATGTTCGTGTTGTGATTAATTGTCTGCTCAAGGGTTGCACAG CTTTGAATTGTGCTTTTCTTGTATTTAGGCTTCAATTTATTCTTGTTAAATTGGCCCACCACACTTTGTG AATCGTCCAAGTATTCGTCCAGCTTCCGTTTAGTTCCAGTTGCCGATGGTTGGTTCACACCAACAGGATG CTCAAAAGATTCCGCATTATAAGCAGAACTGGGCGATGGTTGCTCCGCAACAGGCAGCTCAAAAGATTCC GCATTATAAGCAGAACTAACTGCTTCTCCGAGATTATCAGTGGTCTTGAGCAAACATTCCATTATATCGT TATCATCAGTTAACGAATTGACGCTTGCCAAAAAGTTTGAAGCTGCCTGCAGTCTGCTGTCAGATACTAC CGTGTCGGCTCCATCCGGCGTGGGATTGTTATAATAATTCAAATAGTCGTTGGGCTGTTGTTTATCACAA AACTCTGAATAGCCGTTGTCGAACGACGCTCGGGACGGCGTCGGAGCACTGGTGTACGACGCGTTAAAAT TAATTTGCGTCATAGTCGTTTGGTTGTTCACGATCGTGTCCCGCCAATGTCAACTTGCAACTGAAACAAT ATTCAACATGAACGTCAATTTATACTGCCCTAATGGCGAACACGATAATAATATTTTTTTTATTATGCCC TCTAAAACCAATGCGGTTATCGTTTATTTATTCAAATTAGATACAGAACATCCGCCGACATACAATGTTA ATGCAAAAACTCGTTTGGTGAGCGGATACGAAAACAGTCGGCCGATAAACATTAATCTGAGGTCGATAAC ACCGTCCTTGAACGGAACACGAGGAGCGTACGTGATCAGCTGCATTCGCGCGCCGCGCCTTTATCGAGAT TTATTTACATACAACAAGTACACTGCGCCGTTGGCATTTGTGGTAACGCGCACACAAGCAGAGCTGCAAGTGTGGCACATTTTGTCTGTGCGCAAAACCTTTGAAGCCAAAAGCACAAGGTCCGTTACGGGCATGCTAGC GCACACGGACAACGGACCCGACAAATTCTACGCCAAGGATTTAATGATAATGTCGGGCAACGTGTCGGTG CATTTTATTAATAACTTACAAAATGTCGCGCGCATCACAAAGACATTGATATATTTAAACATTTATGTCC CGAACTGCAAATCGATCGATAAGCTTGATATCGAATTCCTGCAGCCCAATATTACGTTCGTGCCAGAAAT TAATTTCTCCGCGTCGTATTATACGATTTATACGGTACAGCAGCTTGGCCCACAAATAGATCGTTTTATG ATTTTGATGATGGAGGTGCGCTCAAGATGAAACCCATTCAGACGTTATTAGTTGCGTCAAGTATTTGGCA ATTTGCTACGACGCAATTATTGTGGAAGAAGCGTAATTTGTGAACAGCCCATTCGAGGCTAGATTGAAAA AGTATATTGATATTAAATCATATAAATTGTTTATGAGGCCTTCAAACGAATCTTGTAAAGATTATTTATT AAAATTGTTCAACGATTGTATGAGAGGGTCATTTGTTTTTCAAAACTGAACTCGCTTTACGAGTAGAATT CTACTTGTAAAACACAATCAAGAGATGATGTCATTTGTTTTTCAAAACTGAATGATGTCATTTGTTTTTT AAAACTAAACTCGCTTTTACGAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATTTGTTT TTCAAAACTGAACCGGCTTTACGAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATCATT AAACTGATGTCATTTTTATACACGATTGTTAACATGTTTAATAATGACTAATTTGTTTTTCAAATTAAAC TCGCTTTACAAGTAGAATTCTACTTGTAACGCACGATTAAAATTATTATAATCAGGAATGATGTCATTTG TTTTCGTCATAAAATGTTTATACAACGGAATCTTCTTGTAAATTATCCAAATAATATAATTTATCCGATT CTACGTTACATTTAAATTCGTTGTTATCGTACAATTCTTCAGGACACGCCATGTATTGGCCGTTTTTAAC GTGCAACCAACGATTGTATTTGACGCCGTCGTTGGATTGCGTGTTCAGGTTGGCGTACACGTGACTGGGC ACGGCTTCTTTTTTTACCACTATCGCATCTTCGTCGTACGCGGATCTACAACCAATCCCGTTGCCCACAT AAGCGTACGCGTTTAAAACGTGCGATAGGTCTTTGGCCAATTCGCAATCAGCGTCCACTTTAACGTTGTT GCGTAACTCGTTTAAAGCATTAATAATGACGTCATTTTCCGCATGACAACTGGTTAGCTTGAAAAACGGA ACCGAGTAGTGGCATGAATAAAATAAATCTTTGTTGTCTAATATTGGGGGGGAGCTCTTGTGAGTCCTCG CGGGTAGGTACCACCACCCTGCCTATTTCTGCCGTGAAGCAGTAATGCGTTTCGGTTTGAAGAGTGGGGC GGCCGTGGTACTGAGACCTTAGAACTCATATCTGAAGGTGGGTGGCACATTTACGTTGTAGATGTCTATG GGCTCCAGTAACCACTTAACATCAGGTGGGCTGTGAGCTCTTACACCCATCTACGCAATAAAAAATTAAA AATAAATATGTTTGAAGTCCGTAACATAGATTCCGTATTTTTACAGTTGTTTTTCACGTTTTTCATTTCT TCACCGACAATGGAAAATAATCACACACAAATACACTGTATAGTAACAACGAGCAGAGCCGATTTTGGAG TTTCGATAAAGCGAGGCTACCAAGAATGCGGCAGATAAGATTTACGTACATTCAAGAGTCGCTGATAACA ACTTTTACCTCTCAAATTGCCCACAGTGCGATCACAAGAAACATAGACGAACGGATCTGTGCGCAACGAG CCGCTACGATATCATTATCATACAGATTTTTATCTTTTCATCTAGCTTCAGTTAGTGATGCTTTCTGATC TCTTCATAATTATAATTAAAAAGAATAAATTATCTAGTAATATAGTTCTACTACGGTACACGAATTTTGA GATTAATTAACCGGATTTTCTGGGTTATGATTTACATCGGTACAGAATCTAGTGAAAGCACGTCGAGTGA AATTCTATGAAACTTCGGCGGGAGTCGGGGAGAGGTTACAAGCGACCGCGAGGTGCCGCTAACTTAATCA GTTATCAAGGCATCGCCTTATCAAAAGATGCGAGCTGATAGCGTGCGCGTTACCATATATGGTGACAAAA ACTGAGTCAGCCCGCGATTGGTGGAAAAACAAACTGGAGCCGATACTGTGTAAATTGTGATAACGGCTCT TTTATATAGTTTATCCTCACGAGTCGGTTCTCATTTACTAAGGTGTGCTCGAACAGTGCGCATTCGCATC TACGTACTTGTCACTTATTTAATAATACTATGTAAGTTTTAATTTTAAAATTGCGAAAGAAAAAAAAACA TATTTATTTATTTGTAAAATTTGAATTTCGAAGGTTCTCCGTCCCTTTACCTTTAAGTATTACATATGTT TGAGTGTTTTTTTTTTTTAATAATACGCTAATGATAACGTGTTACGTTACATAATTGTTGCATAACTAGT GAAGTGAAATTTTTTATAAAAAAAAACATTTTTCGGAATTTAGTGTACTGCAGATGTTAATAAACACTAC TAAATAAGAAATAAGTTTATTGGACGCACATTTCAAAGTGTCCACTCGCATCGATCAATTCGGAAACAGA AATTGGGAACAGTGAATTATGAATCTTATACAGTTTTCTTTAACGTCACTAAATAGATGGACGCAAATAA ATTTGTCGTTTACTTAGTATAATGTATGGAATGAGAATGTAGTTTGAATTGTTTTTTTTCTTTTCTTGCA GACTAATTCAAGAGGTGCGACGAAGAAGTTGCCGCGTTGGTAGTAGACGGTATCGATAAGCTTGATATCG AATTCCTGCAGCCCAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAA CATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA TGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATC ACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTG CTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAA GCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATG GCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAA CTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCA AGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTC TCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCC CCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCA TCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGG CAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTG ATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAG CAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTC CGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAAT CAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACT GGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGA GTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAA AGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATG GCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAA ACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCG ACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAA TATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATCGACCCGGGT CTAGAGCGGCCGCCTCGAGAAGTCTTACGAACTTCCCGACGGTCAGGTCATCACCATCGGAAACGAAAGA TTCCGTTGCCCAGAGGCCCTCTTCCAACCCTCGTTCTTGGGTATGGAAGCCAACGGAATCCACGAAACCA CATACAACTCCATCATGAAGTGCGACGTGGACATCCGTAAGGACTTGTACGCCAACACCGTATTGTCCGG TGGTACCACCATGTACCCTGGAATCGCCGACCGTATGCAAAAGGAAATCACACGTCTCGCCCCATCGACA ATGAAGATTAAGATCATCGCTCCCCCAGAGAGGAAGTACTCCGTATGGATCGGTGGATCGATCCTCGCCT CCCTCTCTACCTTCCAACAGATGTGGATCTCGAAACAGGAGTACGACGAGTCTGGTCCCTCCATTGTACA CAGGAAGTGCTTCTAAGCGTTGAGACTTTAAGTTATGATGCCCTACAGCAGAACCTCAAGAGGGTGGCTC AAATTACGCTTGTGATCTTGTAAATAAATTCAGTATTTAATGTAGGTTGTAAGGTATTGTAATATGCATA TTACGTAAAACGAACGGAATGTTGTTGTTGCCGTTTTTTTTTTGACAAAGATTTTTATTTATTAAAGTTA CTAACCCCAAAACTTTTTAATAAAATAAATTTATATACCGGTATAATAACTGACGTTTTTCACTTGCTGT CCCCGCTCCCGACTAACAGTACGTCGTGTGCACCGAAATTACCGATTTCGTACACCGTTTGAGACAGTTA CGCTAGGAGCACAAATCTCCCAGCTGCATACCGTTGTTTACTGCAGCTGCAGTCTTTAATTGGAATGCGA GTCGTTGACCGCTTAATACGAAACATTCTAAAATTCGCAAAATGCAAAGGAAACTGGTTCTGTACTTTCT ACCTTTCAAAAGATTCACCAAATTAATTTTATGCGGACTCACTAATTCCGTAGAAATCTGTGTAGAGGTA CCCAGGTTACGCTTAGGCATAAGATGACTGTTCGCGTTTTTACAATACATACGAGCAGGTTACACACAAG ATGAACATCCTTTGATGCGTCTGTGTCTTGACCCGTCTGAGATTTGAGTGACTTGTCAACGTCATTGCGT AGTGTCACCGGTCGTCGAGATCCCCGCCGCGGTGGAGCTACGAGCTCCAGCTTTTGTTCCCTTTAGTGAG GGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAT TCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACA TTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCG CTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCA GGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTG GCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTT CCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGT TCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCG CCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTAC CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTT TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTA GATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGT TACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGAC TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCG AGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGT GGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGC CAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTAT GGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCG GTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGG CAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAAC CAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACC GCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGA TCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTAC TTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACA CGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCA TGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAA AGTGCCACCTGGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCT CATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTT GAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAA ACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCC GTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGT GGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTG CGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGC TGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGT GAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATT GCAGGTCGATATTTAAAAAAAATTATAATAATGTTAAAGTTGCTTCATACGTTGAAGTACCTTACACAAC AAATAATGCAGACGTCGAAATGACTGACATAACAAATGCGCCCTTTCGCCCAAAATCTAAAGCAAGGAGA AGATTAGATTTTACCAACATGGCGCCGCAGCCGTGTTGTATTGACGACGGCAATTTTGCAAAACTTTACT GTGATATATTTATTAAATTAAGTTTGCTTTAAAATGAGTTTTTTTACAAATCTTCGCAGAGTCAATAAAT TGTATCCTAATCAGGCCAGTTTTCTTGCTGATAATACGCGTCTTTTAACAAGGCACTCCCGCCGGTTTCA CAAATGTGCTCAACGCGCCCAGTGTACGCAACCTTGGAAACAACAGATATGGGCCGGGCTATCAATTATCTAACAACCGGTTTGTGAGCACTTCAGACATAAACAGAATTACTCGTAACAACGATGTCCCCAACATACGC GGAGTATTTCAGGGCATTTCAGACCCTCAAATAAACTCATTGAGCCAATTGCGGCGCATGGACCAACGTG CCAGACTTTCATTACCACACCAAACAGACGCGATCCAATGCAGTCAGACAAAACTTCCCGGAGACCAACG TGCGCACGCCCGAAGGTGTTCAAAATGCACTGCAGCAAAACCCCCCCGTTTACATAATCACATGAGAACC TTGAAAGTAGCAGGAGTGGGCATACTCTTGGGCCGGCGGCGGTTATCTTTTGTTTACCGCCGCCACATTA GTACAAGATATAATCAACGCCATCAATAGAACCGGCGGAAGTTATTATGTGCAAGGTAGAAACGCCGGAG AAAACGCCGAGGCCTGTTTGTTATTGCAGCGCACTTGTCGTCAAGACCGCAATCTAGCTCAGTCGGATGT TAACATTTGCTCAAGAGACCCCTTGTTGGCTAACGATTCGCCCCTACTAACCAACATGTGCCAAGGATTT AACTATGAAACAGAAAAAACAGTTTGTCGCGGCAGCAATCCGGCCGCTAACCCAACTTCGCCTCAATACG TAGATATTAGCGATCTTCTGCGGGCCAAACAATCATGTGCATCGAACCTTACACGTTTAGTGATTTAATT GGCGACTTGCGTTTACATTGGTTACTGGGAAGAGAAGGTTTAATCGGCAAATCGTCCAACGGTAGTGACA GCATCCGCAACAAAATAATGCCTCATCATTATGATGATAGGCGCGTTCTTGTTTTTAGGTTTAATACTTT ATTTTATCTACAGATACATGACAAAAGGAGGAGGAGGAGGAGGAAGCGGTGGGGCACCAACTCCCATTGT TGTTATTATGCAACACCCCACATCAACAGCGGCCCCTCGTCGATAATAAAAGACAAAAATAATATAAAAT ATATGTATAATTAATTAAATTCAAAAGAT其他昆虫表达载体：pMT/BioEase-DEST pVL1392 pVL1393 pXINSECT-DEST39 pXINSECT-DEST38 pBacPAK9 pBacPAK8 pIB/V5-His-TOPO pIEX/Bac-1 pFastBacHT B pFastBacHT A pFastBac1pMT/V5-His-TOPO pMT/BiP/V5-His A pCoHygro pAc5.1/V5-His C pAc5.1-V5-His A pAc5/V5-HisA pAc5-V5-HisB pAc5-V5-HisC pCoBlast pAc5.1/V5-His B pIZ/V5-His pIB/V5-HispIZT/V5-His pMIB/V5-His C pMIB/V5-His A pMT/BiP/V5-His C pMT/BiP/V5-His B pIEx/Bac-4 pIEx/Bac-3 pIEXBac-c-EGFP-3 pIEXBac-c-EGFP-1 pIEXBac-c-EGFP-4 pAc5.1B-EGFP pBlueBacHis2 A pBlueBacHis2 C pBlueBacHis2 B pFastBacHT C pMT-Bip-V5-HisA pFBDM pUCDM pFastBac Dual pMIB/V5-His BpMT/V5-His C pMT/V5-His B pMT/V5-His A pAcGP67ApAc5.1b pAc5.1a pVL1392-XyIE control vector pFastBac-c-His-TEV pFastBac-N-GST-TEV pFastBacI-Gus pFastBacHT-CAT pBADZ-His6Cre。
pAc5.1a编号 载体名称北京华越洋生物KCVECT126 pAc5.1apAc5.1a载体基本信息载体名称: pAc5.1a质粒类型: 昆虫细胞表达载体高拷贝/低拷贝: --启动子: --克隆方法: 多克隆位点，限制性内切酶载体大小: --5' 测序引物及序列: --3' 测序引物及序列: --载体标签: --载体抗性: --筛选标记: --备注: --稳定性: --组成型: --病毒/非病毒: --pAc5.1a其他昆虫表达载体：pMT/BioEase-DEST pVL1392 pVL1393 pXINSECT-DEST39 pXINSECT-DEST38 pBacPAK9 pBacPAK8 pIB/V5-His-TOPO pIEX/Bac-1 pFastBacHT B pFastBacHT A pFastBac1pMT/V5-His-TOPO pMT/BiP/V5-His A pCoHygro pAc5.1/V5-His C pAc5.1-V5-His A pAc5/V5-HisA pAc5-V5-HisB pAc5-V5-HisC pCoBlast pAc5.1/V5-His B pIZ/V5-His pIB/V5-HispIZT/V5-His pMIB/V5-His C pMIB/V5-His A pMT/BiP/V5-His C pMT/BiP/V5-His B pIEx/Bac-4 pIEx/Bac-3 pIEXBac-c-EGFP-3 pIEXBac-c-EGFP-1 pIEXBac-c-EGFP-4 pAc5.1B-EGFP pBlueBacHis2 A pBlueBacHis2 C pBlueBacHis2 B pFastBacHT C pMT-Bip-V5-HisA pFBDM pUCDM pFastBac Dual pMIB/V5-His B pMT/V5-His C pMT/V5-His B pMT/V5-His A pAcGP67ApAc5.1b pAc5.1a pVL1392-XyIE control vector pFastBac-c-His-TEV pFastBac-N-GST-TEV pFastBacI-Gus pFastBacHT-CAT pBADZ-His6Cre。
pFastBacI-Gus编号 载体名称北京华越洋生物KCVECT127 pFastBacI-‐GuspFastBacI-‐Gus载体基本信息载体名称: pFastBacI-Gus质粒类型: 昆虫细胞表达载体高拷贝/低拷贝: --启动子: --克隆方法: 多克隆位点，限制性内切酶载体大小: 6661bp5' 测序引物及序列: --3' 测序引物及序列: --载体标签: --载体抗性: Ampicillin筛选标记: Gentamicin备注: --稳定性: --组成型: --病毒/非病毒: 杆状病毒pFastBacI-‐Gus载体质粒图谱和多克隆位点信息pFastBacI-‐Gus载体简介pFastBac1-‐Gus is a 6661 bp control vector containing the Arabidopsis thaliana gene for β-‐glucuronidase (Gus) (Kertbundit et al., 1991), and was generated by restriction cloning of t he G us g ene i nto p FastBac1. T he m olecular w eight o fβ-‐glucuronidase i s 68.5 k Da.pFastBacI-‐Gus其他昆虫表达载体：pMT/BioEase-DEST pVL1392 pVL1393 pXINSECT-DEST39 pXINSECT-DEST38 pBacPAK9 pBacPAK8 pIB/V5-His-TOPO pIEX/Bac-1 pFastBacHT B pFastBacHT A pFastBac1pMT/V5-His-TOPO pMT/BiP/V5-His A pCoHygro pAc5.1/V5-His C pAc5.1-V5-His A pAc5/V5-HisA pAc5-V5-HisB pAc5-V5-HisC pCoBlast pAc5.1/V5-His B pIZ/V5-His pIB/V5-HispIZT/V5-His pMIB/V5-His C pMIB/V5-His A pMT/BiP/V5-His C pMT/BiP/V5-His B pIEx/Bac-4 pIEx/Bac-3 pIEXBac-c-EGFP-3 pIEXBac-c-EGFP-1 pIEXBac-c-EGFP-4 pAc5.1B-EGFP pBlueBacHis2 A pBlueBacHis2 C pBlueBacHis2 B pFastBacHT C pMT-Bip-V5-HisA pFBDM pUCDM pFastBac Dual pMIB/V5-His B pMT/V5-His C pMT/V5-His B pMT/V5-His A pAcGP67ApAc5.1b pAc5.1a pVL1392-XyIE control vector pFastBac-c-His-TEV pFastBac-N-GST-TEV pFastBacI-Gus pFastBacHT-CAT pBADZ-His6Cre。
Bac-to-Bac杆状病毒表达系统Introduction：Overview：Bac-to-Bac杆状病毒表达系统提供快速有效的方法产生重组杆状病毒。
此方法基于让已经转入杆状病的质粒（杆粒）的位点特意转座子的表达框的质粒在Ecoli中扩增。
Bac-to-Bac杆状病毒表达系统主要包括：*pFastBac捐献质粒的选择，它要能够产生包含目的位点的表达结构，这个目的基因的产生被杆状病毒特意位点启动子控制。
*一个Ecoli宿主，DH10Bac，包含杆状病毒质粒（杆粒）和辅助质粒，在转染pFastBac 表达结构后可以产生重组杆粒。
*一个控制表达的质粒，包括Gus和/或CAT基因，以便在感染细胞后产生重组杆状病毒，表达β-葡萄糖酸酐酶和/或氯霉素乙酰转移酶。
Bac-to-Bac表达系统的优点：使用这个系统产生重组杆状病毒较传统的同源重组有以下优点：*与使用同源重组产生重组杆状病毒所需的4-6周相比，鉴别纯化重组病毒少于两周*减少了从斑点筛选重组病毒DNA所包含亲缘和非重组病毒的几率*可以快速同时进行大量重组，适合表达功能性研究的蛋白选择pFastBac菌体（Vector）：大量的pFastBac菌体都适于进行Bac-to-Bac表达系统。
选择对于你的需要最合适的菌体。
指南用途：指南提供了一个对于Bac-to-Bac表达系统的概述，并对以下提供指导：1、克隆目的基因到pFastBac TM供体质粒的选择2、转化pFastBac TM 结构到最高效的DH10Bac TM产生重组质粒3、转染重组质粒DNA到昆虫细胞产生重组杆状病毒4、扩增滴定（Amplify and titer）杆状病毒株，使用病毒株感染昆虫细胞表达目的重组蛋白重要的：Bac-to-Bac杆状病毒表达系统是用来帮助你产生重组杆状病毒，在昆虫细胞中进行高水平表达目的基因的系统。
虽然他可以帮助你很容易的产生杆状病毒表达你的重组蛋白，但是使用这系统更倾向于有杆状病毒生物学和昆虫表达背景的使用者。
昆虫总抗氧化能力（T-AOC）酶联免疫分析(ELISA)试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定昆虫血清、血浆、组织等样本中总抗氧化能力（T-AOC）的含量。
实验原理：本试剂盒应用双抗体夹心法测定标本中昆虫总抗氧化能力（T-AOC）水平。
用纯化的昆虫总抗氧化能力（T-AOC）捕获抗体包被微孔板，制成固相抗体，往包被的微孔中依次加入昆虫总抗氧化能力（T-AOC），再与HRP标记的检测抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。
TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。
颜色的深浅和样品中的昆虫总抗氧化能力（T-AOC）呈正相关。
用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中昆虫总抗氧化能力（T-AOC）含量。
注：标准品浓度依次为：24、12、6、3、1.5、0 U/mL.样本处理及要求：1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。
仔细收集上清，保存过程中如出现沉淀，应再次离心。
2. 血浆：应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂，混合10-20分钟后，离心20分钟左右（2000-3000转/分）。
仔细收集上清，保存过程中如有沉淀形成，应该再次离心。
3. 尿液：用无菌管收集，离心20分钟左右（2000-3000转/分）。
仔细收集上清，保存过程中如有沉淀形成，应再次离心。
胸腹水、脑脊液参照实行。
4. 细胞培养上清：检测分泌性的成份时，用无菌管收集。
离心20分钟左右（2000-3000转/分）。
仔细收集上清。
检测细胞内的成份时，用PBS（PH7.2-7.4）稀释细胞悬液，细胞浓度达到100万/ml左右。
通过反复冻融，以使细胞破坏并放出细胞内成份。
离心20分钟左右（2000-3000转/分）。
仔细收集上清。
保存过程中如有沉淀形成，应再次离心。
5. 组织标本：切割标本后，称取重量。
融合gp67信号肽的慈菇蛋白酶抑制剂基因在蚕体内表达
季平;吴祥甫
【期刊名称】《生物工程学报》
【年(卷),期】1999(15)3
【摘要】用ＡｃＭＮＰＶ的ｇｐ６７信号肽与慈菇蛋白酶抑制剂基因（ＡＰＩ２）融合，并整合到昆虫病毒表达载体Ｂｍ－ＢａｃＰＡＫ６中，受多角体蛋白基因启动子控制。
慈菇蛋白酶抑制剂在蚕体内成功地得到了高效表达，比较了ｇｐ６７信号肽和慈菇蛋白酶抑制剂信号肽在昆虫表达系统中对表达产物的影响，发现表达产物都能分泌到血淋巴中，表达量很相似，但这两种信号肽在表达过程中都没有被切除，且不同信号肽对表达产物的生物活性有很大影响。
【总页数】5页(P310-314)
【作者】季平;吴祥甫
【作者单位】苏州大学蚕桑学院;中国科学院上海生物化学研究所
【正文语种】中文
【中图分类】Q75
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5.慈菇蛋白酶抑制剂通过花粉管途径对小麦的导入及转基因植株分析 [J], 牟红梅;刘树俊;周文娟;文玉香;张文俊;魏荣瑄
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pAcGP67-A编号 载体名称北京华越洋生物KCVECT153 pAcGP67-‐ApAcGP67-A载体基本信息载体名称: pAcGP67-A , pAcGP67A质粒类型: 昆虫杆状病毒表达细胞，杆状病毒转移载体启动子: Polyhedrin表达水平: 高克隆方法: 多克隆位点，限制性内切酶载体大小: 9761 bp5' 测序引物: polyhedrin Forward: AAATGATAACCATCTCGC3' 测序引物: polyhedrin Reverse: GTCCAAGTTTCCCTG载体标签: N-GP67 signal载体抗性: 氨苄筛选标记: --备注: 载体含有GP67分泌信号肽。
稳定性: 稳表达组成型: 非组成型病毒/非病毒: 杆状病毒pAcGP67-A载体质粒图谱和多克隆位点信息pAcGP67-A载体简介酸性糖蛋白gp67（又名gp64）是AcNPV杆状病毒上最丰富的信封表面糖蛋白，是杆状病毒粒子进入易感染的昆虫细胞的必需蛋白。
由于大量的该蛋白被细胞分泌出来，并且铆钉在病毒膜粒子上。
该病毒基因包含有一个最有效的杆状病毒编码的蛋白分泌信号肽序列。
所以，我们制备了这个杆状病毒转移载体(pAcGP67-A, B, C),该载体含有gp67蛋白分泌信号肽。
在载体上，该信号肽位于多克隆位点上游。
目的基因可以插入到多克隆位点中，同时利用gp67蛋白分泌信号肽在PH启动子作用下进行蛋白表达。
这使得能够将非分泌蛋白通过分泌的方式进行蛋白表达，并且纯化出来。
pAcGP67-A载体序列ORIGIN1 AAGCTTTACT CGTAAAGCGA GTTGAAGGAT CATATTTAGT TGCGTTTATG AGATAAGATT 61 GAAAGCACGT GTAAAATGTT TCCCGCGCGT TGGCACAACT ATTTACAATG CGGCCAAGTT 121 ATAAAAGATT CTAATCTGAT ATGTTTTAAA ACACCTTTGC GGCCCGAGTT GTTTGCGTAC 181 GTGACTAGCG AAGAAGATGT GTGGACCGCA GAACAGATAG TAAAACAAAA CCCTAGTATT 241 GGAGCAATAA TCGATTTAAC CAACACGTCT AAATATTATG ATGGTGTGCA TTTTTTGCGG 301 GCGGGCCTGT TATACAAAAA AATTCAAGTA CCTGGCCAGA CTTTGCCGCC TGAAAGCATA 361 GTTCAAGAAT TTATTGACAC GGTAAAAGAA TTTACAGAAA AGTGTCCCGG CATGTTGGTG 421 GGCGTGCACT GCACACACGG TATTAATCGC ACCGGTTACA TGGTGTGCAG ATATTTAATG 481 CACACCCTGG GTATTGCGCC GCAGGAAGCC ATAGATAGAT TCGAAAAAGC CAGAGGTCAC 541 AAAATTGAAA GACAAAATTA CGTTCAAGAT TTATTAATTT AATTAATATT ATTTGCATTC 601 TTTAACAAAT ACTTTATCCT ATTTTCAAAT TGTTGCGCTT CTTCCAGCGA ACCAAAACTA 661 TGCTTCGCTT GCTCCGTTTA GCTTGTAGCC GATCAGTGGC GTTGTTCCAA TCGACGGTAG 721 GATTAGGCCG GATATTCTCC ACCACAATGT TGGCAACGTT GATGTTACGT TTATGCTTTT 781 GGTTTTCCAC GTACGTCTTT TGGCCGGTAA TAGCCGTAAA CGTAGTGCCG TCGCGCGTCA 841 CGCACAACAC CGGATGTTTG CGCTTGTCCG CGGGGTATTG AACCGCGCGA TCCGACAAAT 901 CCACCACTTT GGCAACTAAA TCGGTGACCT GCGCGTCTTT TTTCTGCATT ATTTCGTCTT 961 TCTTTTGCAT GGTTTCCTGG AAGCCGGTGT ACATGCGGTT TAGATCAGTC ATGACGCGCG 1021 TGACCTGCAA ATCTTTGGCC TCGATCTGCT TGTCCTTGAT GGCAACGATG CGTTCAATAA 1081 ACTCTTGTTT TTTAACAAGT TCCTCGGTTT TTTGCGCCAC CACCGCTTGC AGCGCGTTTG1141 TGTGCTCGGT GAATGTCGCA ATCAGCTTAG TCACCAACTG TTTGCTCTCC TCCTCCCGTT 1201 GTTTGATCGC GGGATCGTAC TTGCCGGTGC AGAGCACTTG AGGAATTACT TCTTCTAAAA 1261 GCCATTCTTG TAATTCTATG GCGTAAGGCA ATTTGGACTT CATAATCAGC TGAATCACGC 1321 CGGATTTAGT AATGAGCACT GTATGCGGCT GCAAATACAG CGGGTCGCCC CTTTTCACGA 1381 CGCTGTTAGA GGTAGGGCCC CCATTTTGGA TGGTCTGCTC AAATAACGAT TTGTATTTAT 1441 TGTCTACATG AACACGTATA GCTTTATCAC AAACTGTATA TTTTAAACTG TTAGCGACGT 1501 CCTTGGCCAC GAACCGGACC TGTTGGTCGC GCTCTAGCAC GTACCGCAGG TTGAACGTAT 1561 CTTCTCCAAA TTTAAATTCT CCAATTTTAA CGCGAGCCAT TTTGATACAC GTGTGTCGAT 1621 TTTGCAACAA CTATTGTTTT TTAACGCAAA CTAAACTTAT TGTGGTAAGC AATAATTAAA 1681 TATGGGGGAA CATGCGCCGC TACAACACTC GTCGTTATGA ACGCAGACGG CGCCGGTCTC 1741 GGCGCAAGCG GCTAAAACGT GTTGCGCGTT CAACGCGGCA AACATCGCAA AAGCCAATAG 1801 TACAGTTTTG ATTTGCATAT TAACGGCGAT TTTTTAAATT ATCTTATTTA ATAAATAGTT 1861 ATGACGCCTA CAACTCCCCG CCCGCGTTGA CTCGCTGCAC CTCGAGCAGT TCGTTGACGC 1921 CTTCCTCCGT GTGGCCGAAC ACGTCGAGCG GGTGGTCGAT GACCAGCGGC GTGCCGCACG 1981 CGACGCACAA GTATCTGTAC ACCGAATGAT CGTCGGGCGA AGGCACGTCG GCCTCCAAGT 2041 GGCAATATTG GCAAATTCGA AAATATATAC AGTTGGGTTG TTTGCGCATA TCTATCGTGG 2101 CGTTGGGCAT GTACGTCCGA ACGTTGATTT GCATGCAAGC CGAAATTAAA TCATTGCGAT 2161 TAGTGCGATT AAAACGTTGT ACATCCTCGC TTTTAATCAT GCCGTCGATT AAATCGCGCA 2221 ATCGAGTCAA GTGATCAAAG TGTGGAATAA TGTTTTCTTT GTATTCCCGA GTCAAGCGCA 2281 GCGCGTATTT TAACAAACTA GCCATCTTGT AAGTTAGTTT CATTTAATGC AACTTTATCC 2341 AATAATATAT TATGTATCGC ACGTCAAGAA TTAACAATGC GCCCGTTGTC GCATCTCAAC 2401 ACGACTATGA TAGAGATCAA ATAAAGCGCG AATTAAATAG CTTGCGACGC AACGTGCACG 2461 ATCTGTGCAC GCGTTCCGGC ACGAGCTTTG ATTGTAATAA GTTTTTACGA AGCGATGACA 2521 TGACCCCCGT AGTGACAACG ATCACGCCCA AAAGAACTGC CGACTACAAA ATTACCGAGT 2581 ATGTCGGTGA CGTTAAAACT ATTAAGCCAT CCAATCGACC GTTAGTCGAA TCAGGACCGC 2641 TGGTGCGAGA AGCCGCGAAG TATGGCGAAT GCATCGTATA ACGTGTGGAG TCCGCTCATT 2701 AGAGCGTCAT GTTTAGACAA GAAAGCTACA TATTTAATTG ATCCCGATGA TTTTATTGAT 2761 AAATTGACCC TAACTCCATA CACGGTATTC TACAATGGCG GGGTTTTGGT CAAAATTTCC 2821 GGACTGCGAT TGTACATGCT GTTAACGGCT CCGCCCACTA TTAATGAAAT TAAAAATTCC 2881 AATTTTAAAA AACGCAGCAA GAGAAACATT TGTATGAAAG AATGCGTAGA AGGAAAGAAA 2941 AATGTCGTCG ACATGCTGAA CAACAAGATT AATATGCCTC CGTGTATAAA AAAAATATTG 3001 AACGATTTGA AAGAAAACAA TGTACCGCGC GGCGGTATGT ACAGGAAGAG GTTTATACTA 3061 AACTGTTACA TTGCAAACGT GGTTTCGTGT GCCAAGTGTG AAAACCGATG TTTAATCAAG 3121 GCTCTGACGC ATTTCTACAA CCACGACTCC AAGTGTGTGG GTGAAGTCAT GCATCTTTTA 3181 ATCAAATCCC AAGATGTGTA TAAACCACCA AACTGCCAAA AAATGAAAAC TGTCGACAAG 3241 CTCTGTCCGT TTGCTGGCAA CTGCAAGGGT CTCAATCCTA TTTGTAATTA TTGAATAATA 3301 AAACAATTAT AAATGCTAAA TTTGTTTTTT ATTAACGATA CAAACCAAAC GCAACAAGAA 3361 CATTTGTAGT ATTATCTATA ATTGAAAACG CGTAGTTATA ATCGCTGAGG TAATATTTAA 3421 AATCATTTTC AAATGATTCA CAGTTAATTT GCGACAATAT AATTTTATTT TCACATAAAC 3481 TAGACGCCTT GTCGTCTTCT TCTTCGTATT CCTTCTCTTT TTCATTTTTC TCCTCATAAA 3541 AATTAACATA GTTATTATCG TATCCATATA TGTATCTATC GTATAGAGTA AATTTTTTGT 3601 TGTCATAAAT ATATATGTCT TTTTTAATGG GGTGTATAGT ACCGCTGCGC ATAGTTTTTC 3661 TGTAATTTAC AACAGTGCTA TTTTCTGGTA GTTCTTCGGA GTGTGTTGCT TTAATTATTA 3721 AATTTATATA ATCAATGAAT TTGGGATCGT CGGTTTTGTA CAATATGTTG CCGGCATAGT3781 ACGCAGCTTC TTCTAGTTCA ATTACACCAT TTTTTAGCAG CACCGGATTA ACATAACTTT 3841 CCAAAATGTT GTACGAACCG TTAAACAAAA ACAGTTCACC TCCCTTTTCT ATACTATTGT 3901 CTGCGAGCAG TTGTTTGTTG TTAAAAATAA CAGCCATTGT AATGAGACGC ACAAACTAAT 3961 ATCACAAACT GGAAATGTCT ATCAATATAT AGTTGCTGAT ATCATGGAGA TAATTAAAAT 4021 GATAACCATC TCGCAAATAA ATAAGTATTT TACTGTTTTC GTAACAGTTT TGTAATAAAA 4081 AAACCTATAA ATATtCCGGA TTATTCATAC CGTCCCACCA TCGGGCGcGG ATCTATGCTA 4141 CTAGTAAATC AGTCACACCA AGGCTTCAAT AAGGAACACA CAAGCAAGAT GGTAAGCGCT 4201 ATTGTTTTAT ATGTGCTTTT GGCGGCGGCG GCGCATTCTG CCTTTGCGGC GGATCCCGGG 4261 TACCTTCTAG AATTCCGGAG CGGCCGCTGC AGATCTgatc cTTTCCTGGG ACCCGGCAAG 4321 AACCAAAAAC TCACTCTCTT CAAGGAAATC CGTAATGTTA AACCCGACAC GATGAAGCTT 4381 GTCGTTGGAT GGAAAGGAAA AGAGTTCTAC AGGGAAACTT GGACCCGCTT CATGGAAGAC 4441 AGCTTCCCCA TTGTTAACGA CCAAGAAGTG ATGGATGTTT TCCTTGTTGT CAACATGCGT 4501 CCCACTAGAC CCAACCGTTG TTACAAATTC CTGGCCCAAC ACGCTCTGCG TTGCGACCCC 4561 GACTATGTAC CTCATGACGT GATTAGGATC GTCGAGCCTT CATGGGTGGG CAGCAACAAC 4621 GAGTACCGCA TCAGCCTGGC TAAGAAGGGC GGCGGCTGCC CAATAATGAA CCTTCACTCT 4681 GAGTACACCA ACTCGTTCGA ACAGTTCATC GATCGTGTCA TCTGGGAGAA CTTCTACAAG 4741 CCCATCGTTT ACATCGGTAC CGACTCTGCT GAAGAGGAGG AAATTCTCCT TGAAGTTTCC 4801 CTGGTGTTCA AAGTAAAGGA GTTTGCACCA GACGCACCTC TGTTCACTGG TCCGGCGTAT 4861 TAAAACACGA TACATTGTTA TTAGTACATT TATTAAGCGC TAGATTCTGT GCGTTGTTGA 4921 TTTACAGACA ATTGTTGTAC GTATTTTAAT AATTCATTAA ATTTATAATC TTTAGGGTGG 4981 TATGTTAGAG CGAAAATCAA ATGATTTTCA GCGTCTTTAT ATCTGAATTT AAATATTAAA 5041 TCCTCAATAG ATTTGTAAAA TAGGTTTCGA TTAGTTTCAA ACAAGGGTTG TTTTTCCGAA 5101 CCGATGGCTG GACTATCTAA TGGATTTTCG CTCAACGCCA CAAAACTTGC CAAATCTTGT 5161 AGCAGCAATC TAGCTTTGTC GATATTCGTT TGTGTTTTGT TTTGTAATAA AGGTTCGACG 5221 TCGTTCAAAA TATTATGCGC TTTTGTATTT CTTTCATCAC TGTCGTTAGT GTACAATTGA 5281 CTCGACGTAA ACACGTTAAA TAAAGCTTGG ACATATTTAA CATCGGGCGT GTTAGCTTTA 5341 TTAGGCCGAT TATCGTCGTC GTCCCAACCC TCGTCGTTAG AAGTTGCTTC CGAAGACGAT 5401 TTTGCCATAG CCACACGACG CCTATTAATT GTGTCGGCTA ACACGTCCGC GATCAAATTT 5461 GTAGTTGAGC TTTTTGGAAT TATTTCTGAT TGCGGGCGTT TTTGGGCGGG TTTCAATCTA 5521 ACTGTGCCCG ATTTTAATTC AGACAACACG TTAGAAAGCG ATGGTGCAGG CGGTGGTAAC 5581 ATTTCAGACG GCAAATCTAC TAATGGCGGC GGTGGTGGAG CTGATGATAA ATCTACCATC 5641 GGTGGAGGCG CAGGCGGGGC TGGCGGCGGA GGCGGAGGCG GAGGTGGTGG CGGTGATGCA 5701 GACGGCGGTT TAGGCTCAAA TGTCTCTTTA GGCAACACAG TCGGCACCTC AACTATTGTA 5761 CTGGTTTCGG GCGCCGTTTT TGGTTTGACC GGTCTGAGAC GAGTGCGATT TTTTTCGTTT 5821 CTAATAGCTT CCAACAATTG TTGTCTGTCG TCTAAAGGTG CAGCGGGTTG AGGTTCCGTC 5881 GGCATTGGTG GAGCGGGCGG CAATTCAGAC ATCGATGGTG GTGGTGGTGG TGGAGGCGCT 5941 GGAATGTTAG GCACGGGAGA AGGTGGTGGC GGCGGTGCCG CCGGTATAAT TTGTTCTGGT 6001 TTAGTTTGTT CGCGCACGAT TGTGGGCACC GGCGCAGGCG CCGCTGGCTG CACAACGGAA 6061 GGTCGTCTGC TTCGAGGCAG CGCTTGGGGT GGTGGCAATT CAATATTATA ATTGGAATAC 6121 AAATCGTAAA AATCTGCTAT AAGCATTGTA ATTTCGCTAT CGTTTACCGT GCCGATATTT 6181 AACAACCGCT CAATGTAAGC AATTGTATTG TAAAGAGATT GTCTCAAGCT CCGCACGCCG 6241 ATAACAAGCC TTTTCATTTT TACTACAGCA TTGTAGTGGC GAGACACTTC GCTGTCGTCG 6301 ACGTACATGT ATGCTTTGTT GTCAAAAACG TCGTTGGCAA GCTTTAAAAT ATTTAAAAGA 6361 ACATCTCTGT TCAGCACCAC TGTGTTGTCG TAAATGTTGT TTTTGATAAT TTGCGCTTCC6421 GCAGTATCGA CACGTTCAAA AAATTGATGC GCATCAATTT TGTTGTTCCT ATTATTGAAT 6481 AAATAAGATT GTACAGATTC ATATCTACGA TTCGTCATGG CCACCACAAA TGCTACGCTG 6541 CAAACGCTGG TACAATTTTA CGAAAACTGC AAAAACGTCA AAACTCGGTA TAAAATAATC 6601 AACGGGCGCT TTGGCAAAAT ATCTATTTTA TCGCACAAGC CCACTAGCAA ATTGTATTTG 6661 CAGAAAACAA TTTCGGCGCA CAATTTTAAC GCTGACGAAA TAAAAGTTCA CCAGTTAATG 6721 AGCGACCACC CAAATTTTAT AAAAATCTAT TTTAATCACG GTTCCATCAA CAACCAAGTG 6781 ATCGTGATGG ACTACATTGA CTGTCCCGAT TTATTTGAAA CACTACAAAT TAAAGGCGAG 6841 CTTTCGTACC AACTTGTTAG CAATATTATT AGACAGCTGT GTGAAGCGCT CAACGATTTG 6901 CACAAGCACA ATTTCATACA CAACGACATA AAACTCGAAA ATGTCTTATA TTTCGAAGCA 6961 CTTGATCGCG TGTATGTTTG CGATTACGGA TTGTGCAAAC ACGAAAACTC ACTTAGCGTG 7021 CACGACGGCA CGTTGGAGTA TTTTAGTCCG GAAAAAATTC GACACACAAC TATGCACGTT 7081 TCGTTTGACT GGTACGCGGC GTGTTAACAT ACAAGTTGCT AACCGGcggt tcGTAATCAT 7141 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 7201 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 7261 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 7321 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 7381 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 7441 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 7501 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 7561 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 7621 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 7681 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 7741 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 7801 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 7861 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 7921 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 7981 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 8041 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 8101 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 8161 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 8221 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 8281 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 8341 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 8401 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 8461 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 8521 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 8581 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 8641 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 8701 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 8761 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 8821 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 8881 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 8941 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 9001 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT9061 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 9121 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 9181 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 9241 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT 9301 AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC 9361 GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG 9421 TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG 9481 GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG 9541 TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC 9601 GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC 9661 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG 9721 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC C//其他昆虫表达载体：pMT/BioEase-DEST pVL1392 pVL1393 pXINSECT-DEST39 pXINSECT-DEST38 pBacPAK9 pBacPAK8 pIB/V5-His-TOPO pIEX/Bac-1 pFastBacHT B pFastBacHT A pFastBac1pMT/V5-His-TOPO pMT/BiP/V5-His A pCoHygro pAc5.1/V5-His C pAc5.1-V5-His A pAc5/V5-HisA pAc5-V5-HisB pAc5-V5-HisC pCoBlast pAc5.1/V5-His B pIZ/V5-His pIB/V5-HispIZT/V5-His pMIB/V5-His C pMIB/V5-His A pMT/BiP/V5-His C pMT/BiP/V5-His B pIEx/Bac-4 pIEx/Bac-3 pIEXBac-c-EGFP-3 pIEXBac-c-EGFP-1 pIEXBac-c-EGFP-4 pAc5.1B-EGFP pBlueBacHis2 A pBlueBacHis2 C pBlueBacHis2 B pFastBacHT C pMT-Bip-V5-HisA pFBDM pUCDM pFastBac Dual pMIB/V5-His B pMT/V5-His C pMT/V5-His B pMT/V5-His A pAcGP67ApAc5.1b pAc5.1a pVL1392-XyIE control vector pFastBac-c-His-TEV pFastBac-N-GST-TEV pFastBacI-Gus pFastBacHT-CAT pBADZ-His6Cre。
